The Experts below are selected from a list of 909 Experts worldwide ranked by ideXlab platform
Cristina Giatti Marques De Souza - One of the best experts on this subject based on the ideXlab platform.
-
biodegradation of human keratin by protease from the basidiomycete Pleurotus pulmonarius
International Biodeterioration & Biodegradation, 2018Co-Authors: Rosane Marina Peralta, Tatiane Brugnari, Fabiola Dorneles Inacio, Alessandro F Martins, Alex Graca Contato, Cristina Giatti Marques De SouzaAbstract:Abstract Many microorganisms have been studied for their ability to produce keratinolytic enzymes, among which are basidiomycetes fungi. This paper describes, for the first time, the keratinolytic activity of a protease from Pleurotus pulmonarius, a white rot wood fungus, both in vivo and in vitro, using hair as substrate. The fungus was grown in solid medium containing hair and the production of a protease of 16 kDa with keratinolytic activity was confirmed by solid-state culture. In vitro, tests showed that the protease was able to act on different keratinous substrates. Regarding SEM-EDS, morphological changes were seen in the treated samples with P. pulmonarius in relation to the control, whereas in the FTIR, these alterations were visualized in the spectral regions of the 1044 cm−1 and 1200-400 cm−1, indicating sulfitolysis, which occurs due to the keratinolytic activity of the protease from the fungus P. pulmonaris and consequent degradation of the capillary fibers.
-
removal of bisphenol a by laccases from Pleurotus ostreatus and Pleurotus pulmonarius and evaluation of ecotoxicity of degradation products
Chemical Engineering Journal, 2017Co-Authors: Emanuelle Neiverth De Freitas, Gisele Adriana Bubna, Tatiane Brugnari, Camila Gabriel Kato, Mariene Marques Nolli, Thalita Grando Rauen, Regina De Fatima Peralta Muniz Moreira, Rosely A Peralta, Adelar Bracht, Cristina Giatti Marques De SouzaAbstract:Abstract Bisphenol A (BPA) is an endocrine disruptor compound, that is continuously released into the environment and is barely degraded in wastewater treatment plants. In this work, crude laccases from Pleurotus ostreatus and Pleurotus pulmonarius were used for the first time to degrade BPA in aqueous solution. The residual BPA was quantified by HPLC and the BPA metabolites produced by action of the laccases were identified by GC–MS. BPA at 100 mg/L and 200 mg/L (0.88 mmol/L) were 100% and 85% removed, respectively, in a 1 h reaction by both fungal laccases at a concentration of 8 U/L. Thirteen aromatic and aliphatic BPA metabolites were identified, including p-isopropenylphenol, methylpent-3-oic acid, ethyl-3-ethoxy propanoate, and 4-ethyl-2-methoxyphenol. The acute BPA toxicity decreased from 85% to less than 5% using the P. ostreatus laccase. On the other hand, P. pulmonarius laccase, did not caused reduction in toxicity, possibly because at least one BPA metabolite was as toxic as the parent compound itself. The results of this study suggest a feasible method for the complete removal of BPA from polluted environments using crude laccase from P. ostreatus.
-
production of enzymes and biotransformation of orange waste by oyster mushroom Pleurotus pulmonarius fr quel
Ai Magazine, 2015Co-Authors: Fabiola Dorneles Inacio, Rosane Marina Peralta, Roselene Oliveira Ferreira, Caroline Aparecida Vaz De Araujo, Cristina Giatti Marques De SouzaAbstract:The wood-decay fungi are able to bioconvert a wide variety of lignocellulosic residues due to the secretion of extracellular enzymes. The use of agricultural wastes as substrate for mushroom cultivation or enzymes production can help to solve environmental problems caused by inadequate discharge in the nature. The production of hydrolytic and oxidative enzymes by Pleurotus pulmonarius developed in solid state system using orange waste as substrate was evaluated in this work. Among the hydrolytic enzymes, pectinase was the main enzyme produced by the fungus, presenting the highest enzymatic activity of 9.4 U/mL after 35 days of cultivation. Considering the oxidative enzymes, laccase was the main enzyme produced with maximal activity of 12.2 U/mL obtained after 20 days of cultivation. Low enzyme levels of manganese peroxidase, β-glucosidase and β-xy-losidase were detected with activity peaks at the end of the cultivation. The enzymatic levels of amylase, carboxymethyl cellulase and xylanase were similar and less than 1.5 U/mL. No aryl-alcohol oxidase activity was detected. NDF, ADF and cellulose values increased during 45 days of cultivation. There was no lignin degradation during the study period and the fungus culture in orange solid waste caused protein enrichment in the substrate. Our results demonstrate that P. pulmonarius was an efficient producer of two important industrial enzymes, pectinase and laccase in a cheap solid state system using orange waste as substrate.
-
copper improves the production of laccase by the white rot fungus Pleurotus pulmonarius in solid state fermentation
Brazilian Archives of Biology and Technology, 2006Co-Authors: Giovana Kirst Tychanowicz, Cristina Giatti Marques De Souza, Daniela Farani De Souza, Marina Kimiko Kadowaki, Rosane Marina PeraltaAbstract:Pleurotus pulmonarius (Fr) Quelet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
-
co production of ligninolytic enzymes by Pleurotus pulmonarius on wheat bran solid state cultures
Journal of Basic Microbiology, 2006Co-Authors: Daniela Farani De Souza, Cristina Giatti Marques De Souza, Giovana Kirst Tychanowicz, Rosane Marina PeraltaAbstract:In this paper, the production of biomass and ligninolytic enzymes by the white-rot fungus Pleurotus pulmonarius (Fr.) Quelet cultured on wheat bran at high initial moisture was evaluated. When the initial moisture was lower than 86%, the fungal hyphae penetrated into and bound tightly to the solid-substrate particles. When the moisture was equal or higher than 86%, the growth was characterized by formation of a large mycelial mass above the substrate (surface growth). In this case, the mycelial mass could be easily separated from the residual solid substrate and quantified by gravimetric analysis. The fungus produced 0.32 g of dry mycelial mass per g of dry substrate after 15 days of cultivation. Analysis of the residual substrate showed that growth was mainly due to the consumption of soluble proteins and carbohydrates. The condition of high initial moisture strongly promoted the expression of laccase (up to 24,000 U per g of substrate or 78,000 U per g of dry fungal biomass), while the production of manganese peroxidase was negatively affected. In fact, manganese peroxidase was maximally produced when the initial moisture was 75% (2,000 U per g of substrate). The production of other enzymes, such as polysaccharidases and proteases, was not significantly affected by the initial moisture. (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Rosane Marina Peralta - One of the best experts on this subject based on the ideXlab platform.
-
biodegradation of human keratin by protease from the basidiomycete Pleurotus pulmonarius
International Biodeterioration & Biodegradation, 2018Co-Authors: Rosane Marina Peralta, Tatiane Brugnari, Fabiola Dorneles Inacio, Alessandro F Martins, Alex Graca Contato, Cristina Giatti Marques De SouzaAbstract:Abstract Many microorganisms have been studied for their ability to produce keratinolytic enzymes, among which are basidiomycetes fungi. This paper describes, for the first time, the keratinolytic activity of a protease from Pleurotus pulmonarius, a white rot wood fungus, both in vivo and in vitro, using hair as substrate. The fungus was grown in solid medium containing hair and the production of a protease of 16 kDa with keratinolytic activity was confirmed by solid-state culture. In vitro, tests showed that the protease was able to act on different keratinous substrates. Regarding SEM-EDS, morphological changes were seen in the treated samples with P. pulmonarius in relation to the control, whereas in the FTIR, these alterations were visualized in the spectral regions of the 1044 cm−1 and 1200-400 cm−1, indicating sulfitolysis, which occurs due to the keratinolytic activity of the protease from the fungus P. pulmonaris and consequent degradation of the capillary fibers.
-
Spent mushroom substrate of Pleurotus pulmonarius: a source of easily hydrolyzable lignocellulose
Folia Microbiologica, 2016Co-Authors: Rúbia Carvalho Gomes Corrêa, Camila Gabriel Kato, Adelar Bracht, Bruna Polacchini Silva, Rafael Castoldi, Anacharis Babeto Sá-nakanishi, Rosely Aparecida Peralta, Cristina G. M. Souza, Rosane Marina PeraltaAbstract:Pleurotus pulmonarius was cultivated on a corncob-based substrate for producing of mushrooms and for assessing the transformation of the lignocellulosics during the development of fungal biomass. Associated events, such as the release of relevant enzymes and the H_2O_2 generation, were also monitored. The peaks of laccase and catalase activities occurred at the 5th day and that of Mn peroxidase at the 30th day, simultaneously with a high activity of superoxide dismutase. Increase in the endocellulase and xylanase activities was observed after 10 days, with maximal activities achieved during the 20–30-day period. Maximal values of H_2O_2 were found after 10 days of cultivation. Electron microscopy and Fourier transform infrared (FTIR) spectroscopy showed strong alterations in the lignocellulosic fibers. The uncultivated and the cultivated substrates at different times were hydrolyzed with commercial cellulase and β-glucosidase. The highest values of reducing sugars (110.5 ± 5.6 μmol/mL), being 65 % glucose, were obtained using the 20-day cultivated substrate. After the fruiting stage (first flush), enzymatic hydrolysis of the spent mushroom substrate (SMS) yielded 53.0 ± 2.8 and 77.5 ± 4.0 μmol/mL of glucose and total reducing sugars, respectively. Although the release of reducing sugars of the P. pulmonarius SMS was lower than that obtained after 20 days of cultivation, it was still 50 % higher than that obtained using the uncultured substrate. This observation, combined with the fact that SMS constitutes a residue generated as a by-product of the depletion of an agro-industrial residue, allows to conclude that this material offers an interesting economic perspective for the obtainment of cellulosic ethanol.
-
production of enzymes and biotransformation of orange waste by oyster mushroom Pleurotus pulmonarius fr quel
Ai Magazine, 2015Co-Authors: Fabiola Dorneles Inacio, Rosane Marina Peralta, Roselene Oliveira Ferreira, Caroline Aparecida Vaz De Araujo, Cristina Giatti Marques De SouzaAbstract:The wood-decay fungi are able to bioconvert a wide variety of lignocellulosic residues due to the secretion of extracellular enzymes. The use of agricultural wastes as substrate for mushroom cultivation or enzymes production can help to solve environmental problems caused by inadequate discharge in the nature. The production of hydrolytic and oxidative enzymes by Pleurotus pulmonarius developed in solid state system using orange waste as substrate was evaluated in this work. Among the hydrolytic enzymes, pectinase was the main enzyme produced by the fungus, presenting the highest enzymatic activity of 9.4 U/mL after 35 days of cultivation. Considering the oxidative enzymes, laccase was the main enzyme produced with maximal activity of 12.2 U/mL obtained after 20 days of cultivation. Low enzyme levels of manganese peroxidase, β-glucosidase and β-xy-losidase were detected with activity peaks at the end of the cultivation. The enzymatic levels of amylase, carboxymethyl cellulase and xylanase were similar and less than 1.5 U/mL. No aryl-alcohol oxidase activity was detected. NDF, ADF and cellulose values increased during 45 days of cultivation. There was no lignin degradation during the study period and the fungus culture in orange solid waste caused protein enrichment in the substrate. Our results demonstrate that P. pulmonarius was an efficient producer of two important industrial enzymes, pectinase and laccase in a cheap solid state system using orange waste as substrate.
-
Production of laccase and manganese peroxidase by Pleurotus pulmonarius in solid-state cultures and application in dye decolorization.
Folia Microbiologica, 2013Co-Authors: Gisele Cristina Dos Santos Bazanella, Adelar Bracht, Daniela Farani De Souza, Rafael Castoldi, Roselene Ferreira Oliveira, Rosane Marina PeraltaAbstract:The production of ligninolytic enzymes (laccase and Mn-dependent peroxidase) by the white-rot fungus Pleurotus pulmonarius (FR.) Quelet was studied in solid-state cultures using agricultural and food wastes as substrate. The highest activities of laccase were found in wheat bran (2,860 ± 250 U/L), pineapple peel (2,450 ± 230 U/L), and orange bagasse (2,100 ± 270 U/L) cultures, all of them at an initial moisture level of 85 %. The highest activities of Mn peroxidase were obtained in pineapple peel cultures (2,200 ± 205 U/L) at an initial moisture level of 75 %. In general, the condition of high initial moisture level (80–90 %) was the best condition for laccase activity, while the best condition for Mn peroxidase activity was cultivation at low initial moisture (50–70 %). Cultures containing high Mn peroxidase activities were more efficient in the decolorization of the industrial dyes remazol brilliant blue R (RBBR), Congo red, methylene blue, and ethyl violet than those containing high laccase activity. Also, crude enzymatic extracts with high Mn peroxidase activity were more efficient in the in vitro decolorization of methylene blue, ethyl violet, and Congo red. The dye RBBR was efficiently decolorized by both crude extracts, rich in Mn peroxidase activity or rich in laccase activity.
-
copper improves the production of laccase by the white rot fungus Pleurotus pulmonarius in solid state fermentation
Brazilian Archives of Biology and Technology, 2006Co-Authors: Giovana Kirst Tychanowicz, Cristina Giatti Marques De Souza, Daniela Farani De Souza, Marina Kimiko Kadowaki, Rosane Marina PeraltaAbstract:Pleurotus pulmonarius (Fr) Quelet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Subhash L. Bodhankar - One of the best experts on this subject based on the ideXlab platform.
-
interaction of aqueous extract of Pleurotus pulmonarius fr quel champ with glyburide in alloxan induced diabetic mice
Evidence-based Complementary and Alternative Medicine, 2008Co-Authors: Sachin L. Badole, Naimesh M Patel, Prasad A. Thakurdesai, Subhash L. BodhankarAbstract:Mushrooms are low calorie food with very little fat and are highly suitable for obese persons. With no starch and very low sugars, they are the ‘delight of the diabetics’. Combination of herbal drugs (or isolated phytochemicals) is found to be beneficial in certain diseases when given along with conventional drugs. The aim of the present study was to evaluate the effects of aqueous extract of Pleurotus pulmonarius (Lentinaceae) (called as PP-aqu) and its interaction with glyburide in alloxan induced diabetic mice. The diabetic mice treated were with PP-aqu (500 mg/kg, p.o.) alone or combination with glyburide (10 mg/kg, p.o.) for 28 days. Blood samples were collected by orbital sinus puncture using heparinized capillary glass tubes and were analyzed for serum glucose on 0, 7th, 14th, 21st and 28th days. Body weights and mortality were noted during the study period. In oral glucose tolerance test (OGTT), glucose (2.5 g/kg, p.o.) was administered with either vehicle, PP-aqu alone or in combination with glyburide and serum glucose level analyzed at 0, 30, 60 and 120 min after drug administration. Administration of PP-aqu (500 mg/kg) and its combination with glyburide (10 mg/kg) significantly (P < 0.001) decreased serum glucose level in diabetic mice. In OGTT, glyburide or PP-aqu treatment alone or their combination produced significant (P < 0.001) increase in glucose threshold. Thus we suggest that P. pulmonarius showed potent and synergistic antihyperglycemic effect in combination with glyburide.
-
hypoglycemic activity of aqueous extract of Pleurotus pulmonarius in alloxan induced diabetic mice
Pharmaceutical Biology, 2006Co-Authors: Sachin L. Badole, Naimesh M Patel, Prasad A. Thakurdesai, Shweta Shah, Subhash L. BodhankarAbstract:AbstractMushrooms have been valued throughout the world as both food and medicine for thousands of years. In the current study, we report the hypoglycemic effects of aqueous extract of one variety of mushroom, Pleurotus pulmonarius. (Fr.) Quel.-Champ (Lentinaceae), in alloxan-induced diabetic mice. Pleurotus pulmonarius. extract was administrated orally at doses of 250, 500, and 1000 mg/kg to separate groups of mice (normal and alloxan-treated mice), and serum glucose and body weight were measured. In the separate group of mice, an oral glucose tolerance test (OGTT) was carried out. Acute oral toxicity data showed no mortality in the normal mice up to 5000 mg/kg. Oral administration of extracts reduced the serum glucose level in alloxan-treated diabetic mice in all the doses tested after acute and chronic (28 days) administration. The extract also showed increased glucose tolerance in both normal and diabetic mice. These results suggest that the extract possesses hypoglycemic activity.
-
study of interaction of aqueous extract of Pleurotus pulmonarius fr quel champ with rosiglitazone in alloxan induced diabetic mice
2006Co-Authors: Sachin L. Badole, Subhash L. Bodhankar, Prasad A. ThakurdesaiAbstract:Summary Mushrooms are low calorie food with very little fat, no starch and very low sugars, and so are the “delight of the diabetics”. Recently, we have reported significant hypoglycemic activity of aqueous extract of Pleurotus pulmonarius (Family: Lentinaceae) (PP-aqu) on serum glucose levels and oral glucose tolerance test (OGTT) in alloxan induced diabetic mice at and more than 500 mg/kg, p.o. Herbal agents when given in combination with prescription medication may favorably alter pharmacodynamic activity of prescription medications. Hence, we have evaluated the antihyperglycemic effect of combination of PP-aqu (500 mg/kg, p.o.) with rosiglitazone (6 mg/kg, p.o.) on serum glucose levels and oral glucose tolerance test (OGTT) in alloxan induced diabetic mice. Our results from acute and sub-acute studies suggest that combination treatment of PP-aqu with rosiglitazone has synergistic antihyperglycemic effect than either drug alone. Concurrent drug regimen of PP-aqu and rosiglitazone may be considered for effective diabetes control.
Fabiola Dorneles Inacio - One of the best experts on this subject based on the ideXlab platform.
-
comparison between the aqueous extracts of mycelium and basidioma of the edible mushroom Pleurotus pulmonarius chemical composition and antioxidant analysis
Journal of Food Measurement and Characterization, 2020Co-Authors: Tatiane Brugnari, Fabiola Dorneles Inacio, Caroline Aparecida Vaz De Araujo, Alex Graca Contato, Giselle Maria Maciel, Charles Windson Isidoro Haminiuk, Adelar BrachtAbstract:The aqueous extracts of mycelium (MAE) and basidioma (BAE) of the mushroom Pleurotus pulmonarius (Fr.) Quel. were compared for their chemical composition, phenolic compounds identified using HPLC and antioxidant activities. The MAE contained 66.1 μg of gallic acid, 27.0 μg of ferulic acid and 217 μg of caffeic acid/g dry weight (dw). In addition, 626 μg of gallic acid, 347 μg of caffeic acid, 53.0 μg of vanillic acid and 25.4 μg of ρ-coumaric acid were found in BAE/g dw. The levels of soluble proteins, soluble carbohydrates and free amino acids were higher in the BAE. The MAE of P. pulmonarius were efficient in chelating ferrous ions (EC50 of 1.81 mg dw/mL). The EC50 for DPPH and ABTS radicals were 0.51 and 0.03 mg dw/mL, respectively, showing high antioxidant capacity. The results of this study showed that mycelium and basidioma of P. pulmonarius have the potential to be used as sources of antioxidant compounds and nutrients.
-
biodegradation of human keratin by protease from the basidiomycete Pleurotus pulmonarius
International Biodeterioration & Biodegradation, 2018Co-Authors: Rosane Marina Peralta, Tatiane Brugnari, Fabiola Dorneles Inacio, Alessandro F Martins, Alex Graca Contato, Cristina Giatti Marques De SouzaAbstract:Abstract Many microorganisms have been studied for their ability to produce keratinolytic enzymes, among which are basidiomycetes fungi. This paper describes, for the first time, the keratinolytic activity of a protease from Pleurotus pulmonarius, a white rot wood fungus, both in vivo and in vitro, using hair as substrate. The fungus was grown in solid medium containing hair and the production of a protease of 16 kDa with keratinolytic activity was confirmed by solid-state culture. In vitro, tests showed that the protease was able to act on different keratinous substrates. Regarding SEM-EDS, morphological changes were seen in the treated samples with P. pulmonarius in relation to the control, whereas in the FTIR, these alterations were visualized in the spectral regions of the 1044 cm−1 and 1200-400 cm−1, indicating sulfitolysis, which occurs due to the keratinolytic activity of the protease from the fungus P. pulmonaris and consequent degradation of the capillary fibers.
-
production of enzymes and biotransformation of orange waste by oyster mushroom Pleurotus pulmonarius fr quel
Ai Magazine, 2015Co-Authors: Fabiola Dorneles Inacio, Rosane Marina Peralta, Roselene Oliveira Ferreira, Caroline Aparecida Vaz De Araujo, Cristina Giatti Marques De SouzaAbstract:The wood-decay fungi are able to bioconvert a wide variety of lignocellulosic residues due to the secretion of extracellular enzymes. The use of agricultural wastes as substrate for mushroom cultivation or enzymes production can help to solve environmental problems caused by inadequate discharge in the nature. The production of hydrolytic and oxidative enzymes by Pleurotus pulmonarius developed in solid state system using orange waste as substrate was evaluated in this work. Among the hydrolytic enzymes, pectinase was the main enzyme produced by the fungus, presenting the highest enzymatic activity of 9.4 U/mL after 35 days of cultivation. Considering the oxidative enzymes, laccase was the main enzyme produced with maximal activity of 12.2 U/mL obtained after 20 days of cultivation. Low enzyme levels of manganese peroxidase, β-glucosidase and β-xy-losidase were detected with activity peaks at the end of the cultivation. The enzymatic levels of amylase, carboxymethyl cellulase and xylanase were similar and less than 1.5 U/mL. No aryl-alcohol oxidase activity was detected. NDF, ADF and cellulose values increased during 45 days of cultivation. There was no lignin degradation during the study period and the fungus culture in orange solid waste caused protein enrichment in the substrate. Our results demonstrate that P. pulmonarius was an efficient producer of two important industrial enzymes, pectinase and laccase in a cheap solid state system using orange waste as substrate.
Segula Masaphy - One of the best experts on this subject based on the ideXlab platform.
-
manganese enhanced biotransformation of atrazine by the white rot fungus Pleurotus pulmonarius and its correlation with oxidation activity
Applied and Environmental Microbiology, 1996Co-Authors: Segula Masaphy, Y Henis, Dan LevanonAbstract:Manganese enhanced atrazine transformation by the fungus Pleurotus pulmonarius when added to a liquid culture medium at concentrations of up to 300 microM. Both N-dealkylated and propylhydroxylated metabolites accumulated in the culture medium, with the former accumulating to a greater extent than did the latter. Lipid peroxidation, oxygenase and peroxidase activities, and the cytochrome P-450 concentration increased. In addition, an increase in the spectral interactions between atrazine and components in the cell extract was observed. Antioxidants, mainly nordihydroguaiaretic acid, which inhibits lipoxygenase, peroxidase, and P-450 activities, and piperonyl butoxide, which inhibits P-450 activity, inhibited atrazine transformation by the mycelium. It is suggested that the stimulation of oxidative activity by Mn might be responsible for increasing the biotransformation of atrazine and for nonspecific transformations of other xenobiotic compounds.
-
degradation of atrazine by the lignocellulolytic fungus Pleurotus pulmonarius during solid state fermentation
Bioresource Technology, 1996Co-Authors: Segula Masaphy, Dan Levanon, Y HenisAbstract:The biotransformation of atrazine added to a mixture of cotton and wheat straw (CWS) and inoculated with the white-rot fungus, Pleurotus pulmonarius, was studied, as a proposed system for bioremediation. The concentration of methanol-extractable atrazine was reduced, due to both biological transformation and physical-chemical adsorption to the straw. Only 32% of the total radioactivity added as 14C-ring-labeled atrazine to pasteurized CWS inoculated with Pleurotus was extracted two weeks after fungal colonization, and less than 70% from non-inoculated CWS. The reduction in extractable radioactivity increased with time of incubation. No mineralization of the triazine ring was found during six weeks of incubation, but transformation to two groups of atrazine metabolites, chlorinated and dechlorinated, occurred, as a result of the activity of the fungus inoculated and natural bacterial population. Unextractable radioactivity was recovered after digesting the colonized substrate with H2SO4, indicating adsorption of the herbicide and its metabolites to the straw. The results suggest that this process can be used to detoxify atrazine by both adsorption and biodegradation.
-
microsomal and cytosolic cytochrome p450 mediated benzo a pyrene hydroxylation in Pleurotus pulmonarius
Biotechnology Letters, 1995Co-Authors: Segula Masaphy, Dan Levanon, Y Henis, K Venkateswarlu, Steven L KellyAbstract:Microsomal and soluble fractions of Pleurotus pulmonarius exhibited a reduced carbon monoxide difference spectrum with P450 maxima at 448nm and 450–452nm respectively. Substrate induced Type I spectra were observed on addition of benzo(a)pyrene to both fractions. Benzo(a)pyrene hydroxylation was measured using the aryl hydrocarbon hydroxylase assay and was observed to be P450 dependent as indicated by carbon monoxide inhibition together with the substrate binding characteristics. The activity of the fractions were observed to give Km of 200mM and 660mM and Vmax of 1.25 nmol/min/nmol P450 and 0.57 nmol/min/nmol P450 for the microsomal and cytosolic fractions respectively.
-
effect of medium composition on 1 octen 3 ol formation in submerged cultures of Pleurotus pulmonarius
Applied Microbiology and Biotechnology, 1994Co-Authors: Paula Belinky, Yitzhak Hadar, Segula Masaphy, Dan Levanon, Carlos G DosoretzAbstract:The effect of nitrogen and fatty-acid-rich substrates on the production of 1-octen-3-ol by the edible fungus Pleurotus pulmonarius, during growth in both shaken flask and fermentor cultures, and in-vitro, in post-harvested mycelium, was studied. Addition of soybean flour and soybean oil to the growth medium enhanced 1-octen-3-ol production about sevenfold and doubled the fungal biomass, as compared to that obtained from P. pulmonarius cultured on a defined synthetic medium. A clear relationship between the production of 1-octen-3-ol and lipoxygenase activity was found during the growth of mushroom pellets. The highest in-vitro generation of 1-octen-3-ol was obtained upon addition of exogenous linoleic acid and pure O2 to pellets grown with soybean fluor and soybean oil. This generation was even higher than that of fruiting bodies exposed to the same conditions. These results suggest that lipoxygenase activity and, subsequently, 1-octen-3-ol biosynthesis in P. pulmonarius are enhanced by the presence of substrates containing fatty acids in the growth medium.
-
isolation and characterization of a novel atrazine metabolite produced by the fungus Pleurotus pulmonarius 2 chloro 4 ethylamino 6 1 hydroxyisopropyl amino 1 3 5 triazine
Applied and Environmental Microbiology, 1993Co-Authors: Segula Masaphy, Dan Levanon, Jacob Vaya, Y HenisAbstract:The white rot fungus Pleurotus pulmonarius exhibited metabolism of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) in liquid culture, producing the dealkylated metabolites desethylatrazine, desisopropylatrazine, and desethyl-desisopropylatrazine. A fourth, unknown metabolite was also produced. It was isolated and was identified as 2-chloro-4-ethylamino-6-(1-hydroxyisopropyl)amino-1,3,5-triazine by gas chromatography-mass spectrometry, Fourier transformed infrared spectroscopy, and 1H nuclear magnetic resonance analysis. The structure of this metabolite was confirmed by chemical synthesis of the compound and comparison with the fungally produced metabolite.