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Corinne Clave - One of the best experts on this subject based on the ideXlab platform.
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Combinations of Spok genes create multiple meiotic drivers in Podospora
eLife, 2019Co-Authors: Aaron A Vogan, Corinne Clave, Virginie Coustou, Alfons J M Debets, Eric Bastiaans, Jesper Svedberg, Helene Yvanne, S Lorena Ament-velásquez, Alexandra Granger-farbos, Sven SaupeAbstract:Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the Spok gene family underlies the Psks. The combination of Spok genes at different chromosomal locations defines the spore killer types and creates a killing hierarchy within a population. We identify two novel Spok homologs located within a large (74-167 kbp) region (the Spok block) that resides in different chromosomal locations in different strains. We confirm that the SPOK protein performs both killing and resistance functions and show that these activities are dependent on distinct domains, a predicted nuclease and kinase domain. Genomic and phylogenetic analyses across ascomycetes suggest that the Spok genes disperse through cross-species transfer, and evolve by duplication and diversification within lineages.
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combinations of spok genes create multiple meiotic drivers in Podospora
bioRxiv, 2019Co-Authors: Aaron A Vogan, Corinne Clave, Alexandra Grangerfarbos, Virginie Coustou, Lorena S Amentvelasquez, Alfons J M Debets, Eric Bastiaans, Jesper Svedberg, Helene Yvanne, Sven J. SaupeAbstract:Meiotic drive is the preferential transmission of a particular allele at a given locus during sexual reproduction. The phenomenon is observed as spore killing in a variety of fungal lineages, including Podospora. In natural populations of Podospora anserina, seven spore killers (Psks) have been identified through classical genetic analyses. Here we show that the Spok gene family underlie the Psk spore killers. The combination of the various Spok genes at different chromosomal locations defines the spore killers and creates a killing hierarchy within the same population. We identify two novel Spok homologs that are located within a complex region (the Spok block) that reside in different chromosomal locations in given natural strains. We confirm that the individual SPOK proteins perform both the killing and resistance functions and show that these activities are dependent on distinct domains, a nuclease and a kinase domain respectively. Genomic data and phylogenetic analysis across ascomycetes suggest that the Spok genes disperse via cross-species transfer, and evolve by duplication and diversification within several lineages.
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The Transcriptional Response to Nonself in the Fungus Podospora anserina
G3 (Bethesda Md.), 2013Co-Authors: Frédérique Bidard, Corinne Clave, Sven J. SaupeAbstract:In fungi, heterokaryon incompatibility is a nonself recognition process occurring when filaments of different isolates of the same species fuse. Compatibility is controlled by so-called het loci and fusion of strains of unlike het genotype triggers a complex incompatibility reaction that leads to the death of the fusion cell. Herein, we analyze the transcriptional changes during the incompatibility reaction in Podospora anserina. The incompatibility response was found to be associated with a massive transcriptional reprogramming: 2231 genes were up-regulated by a factor 2 or more during incompatibility. In turn, 2441 genes were down-regulated. HET, NACHT, and HeLo domains previously found to be involved in the control of heterokaryon incompatibility were enriched in the up-regulated gene set. In addition, incompatibility was characterized by an up-regulation of proteolytic and other hydrolytic activities, of secondary metabolism clusters and toxins and effector-like proteins. The up-regulated set was found to be enriched for proteins lacking orthologs in other species and chromosomal distribution of the up-regulated genes was uneven with up-regulated genes residing preferentially in genomic islands and on chromosomes IV and V. There was a significant overlap between regulated genes during incompatibility in P. anserina and Neurospora crassa, indicating similarities in the incompatibility responses in these two species. Globally, this study illustrates that the expression changes occurring during cell fusion incompatibility in P. anserina are in several aspects reminiscent of those described in host-pathogen or symbiotic interactions in other fungal species.
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Cell death by incompatibility in the fungus Podospora
Seminars in cancer biology, 2006Co-Authors: Bérangère Pinan-lucarré, Mathieu Paoletti, Corinne ClaveAbstract:Filamentous fungi are naturally able of somatic fusions. When cells of unlike genotype at specific het loci fuse, non-self recognition operates in the fusion cell and a cell death reaction termed cell death by incompatibility is triggered. In Podospora anserina cell death by incompatibility is characterized by a dramatic vacuolar enlargement, induction of autophagy and cell lysis. Autophagy contributes neither to vacuolar morphological changes nor to cell death but rather protects cells against death. Autophagy could be involved in selective elimination of pro-death signals. Vacuole collapse and cytoplasm acidification might be the cause of cell death by incompatibility.
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accelerated cell death in Podospora autophagy mutants
Eukaryotic Cell, 2005Co-Authors: Berangere Pinanlucarre, Axelle Balguerie, Corinne ClaveAbstract:Although autophagy is characteristic of type II programmed cell death (PCD), its role in cell death is currently debated. Both cell death-promoting and prosurvival roles of autophagy have been reported depending on the organism and the cell type. In filamentous fungi, a cell death reaction known as an incompatibility reaction occurs when cells of unlike genotype fuse. Cell death by incompatibility is characterized by a dramatic vacuolar enlargement and cell lysis. In Podospora anserina, autophagy is induced early during this cell death reaction. Cell death by incompatibility in Podospora is a model of type II PCD used here to assess the role of autophagy in this type of cell death. We have inactivated PaATG1, the Podospora ortholog of the Saccharomyces cerevisiae ATG1 gene involved in the early steps of autophagy in yeast. The ΔPaATG1 mutant displays developmental defects characteristic of abrogated autophagy in Podospora. Using the green fluorescent protein-PaATG8 autophagosome marker, we show that autophagy is abolished in this mutant. Neither cell death by incompatibility nor vacuolization are suppressed in ΔPaATG1 and ΔPaATG8 autophagy mutants, indicating that a vacuolar cell death reaction without autophagy occurs in Podospora. Our results thus provide a novel example of a type II PCD reaction in which autophagy is not the cause of cell death. In addition, we found that cell death is accelerated in ΔPaATG null mutants, suggesting that autophagy has a protective role in this type II PCD reaction.
Fabienne Malagnac - One of the best experts on this subject based on the ideXlab platform.
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size variation of the nonrecombining region on the mating type chromosomes in the fungal Podospora anserina species complex
Molecular Biology and Evolution, 2021Co-Authors: Fanny E Hartmann, Valérie Gautier, Pierre Grognet, Hanna Johannesson, Aaron A Vogan, Sandra Lorena Amentvelasquez, Stephanie Le Prieur, Myriam Berramdane, Alodie Snirc, Fabienne MalagnacAbstract:Sex chromosomes often carry large non-recombining regions that can extend progressively over time, generating evolutionary strata of sequence divergence. However, some sex chromosomes display an incomplete suppression of recombination. Large genomic regions without recombination and evolutionary strata have also been documented around fungal mating-type loci, but have been studied in only a few fungal systems. In the model fungus Podospora anserina (Ascomycota, Sordariomycetes), the reference S strain lacks recombination across a 0.8 Mb region around the mating-type locus. The lack of recombination in this region ensures that nuclei of opposite mating types are packaged into a single ascospore (pseudo-homothallic lifecycle). We found evidence for a lack of recombination around the mating-type locus in the genomes of 10 P. anserina strains and six closely related pseudo-homothallic Podospora species. Importantly, the size of the non-recombining region differed between strains and species, as indicated by the heterozygosity levels around the mating-type locus and experimental selfing. The non-recombining region is probably labile and polymorphic, differing in size and precise location within and between species, resulting in occasional, but infrequent, recombination at a given base pair. This view is also supported by the low divergence between mating types, and the lack of strong linkage disequilibrium, chromosomal rearrangements, trans-specific polymorphism and genomic degeneration. We found a pattern suggestive of evolutionary strata in P. pseudocomata. The observed heterozygosity levels indicate low but non-null outcrossing rates in nature in these pseudo-homothallic fungi. This study adds to our understanding of mating-type chromosome evolution and its relationship to mating systems.
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the taxonomy of the model filamentous fungus Podospora anserina
MycoKeys, 2020Co-Authors: Lorena S Amentvelasquez, Fabienne Malagnac, Sven J. Saupe, Pierre Grognet, Robert Debuchy, Hanna Johannesson, Tatiana Giraud, Alfons J M Debets, Eric Bastiaans, Leonardo PerazareyesAbstract:The filamentous fungus Podospora anserina has been used as a model organism for more than 100 years and has proved to be an invaluable resource in numerous areas of research. Throughout this period, P. anserina has been embroiled in a number of taxonomic controversies regarding the proper name under which it should be called. The most recent taxonomic treatment proposed to change the name of this important species to Triangularia anserina. The results of past name changes of this species indicate that the broader research community is unlikely to accept this change, which will lead to nomenclatural instability and confusion in literature. Here, we review the phylogeny of the species closely related to P. anserina and provide evidence that currently available marker information is insufficient to resolve the relationships amongst many of the lineages. We argue that it is not only premature to propose a new name for P. anserina based on current data, but also that every effort should be made to retain P. anserina as the current name to ensure stability and to minimise confusion in scientific literature. Therefore, we synonymise Triangularia with Podospora and suggest that either the type species of Podospora be moved to P. anserina from P. fimiseda or that all species within the Podosporaceae be placed in the genus Podospora.
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Rab-GDI Complex Dissociation Factor Expressed through Translational Frameshifting in Filamentous Ascomycetes.
PLoS ONE, 2013Co-Authors: Fabienne Malagnac, Céline Fabret, Magali Prigent, Jean-pierre Rousset, Olivier Namy, Philippe SilarAbstract:In the model fungus Podospora anserina, the PaYIP3 gene encoding the orthologue of the Saccharomyces cerevisiae YIP3 Rab-GDI complex dissociation factor expresses two polypeptides, one of which, the long form, is produced through a programmed translation frameshift. Inactivation of PaYIP3 results in slightly delayed growth associated with modification in repartition of fruiting body on the thallus, along with reduced ascospore production on wood. Long and short forms of PaYIP3 are expressed in the mycelium, while only the short form appears expressed in the maturing fruiting body (perithecium). The frameshift has been conserved over the evolution of the Pezizomycotina, lasting for over 400 million years, suggesting that it has an important role in the wild.
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the genome sequence of the model ascomycete fungus Podospora anserina
Genome Biology, 2008Co-Authors: Eric Espagne, Fabienne Malagnac, Olivier Lespinet, Corinne Da Silva, Olivier Jaillon, Betina M Porcel, Arnaud Couloux, Jeanmarc Aury, Beatrice Segurens, Julie PoulainAbstract:Background: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development. Results: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/ splicing machinery generates numerous non-conventional tran scripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved
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Genetic control of an epigenetic cell degeneration syndrome in Podospora anserina
Fungal genetics and biology : FG & B, 2005Co-Authors: Vicki Haedens, Fabienne Malagnac, Philippe SilarAbstract:Filamentous fungi frequently present degenerative processes, whose molecular basis is very often unknown. Here, we present three mutant screens that result in the identification of 29 genes that directly or indirectly control Crippled Growth (CG), an epigenetic cell degeneration of the filamentous ascomycete Podospora anserina. Two of these genes were previously shown to encode a MAP kinase kinase kinase and an NADPH oxidase involved in a signal transduction cascade that participates in stationary phase differentiations, fruiting body development and defence against fungal competitors. The numerous genes identified can be incorporated in a model in which CG results from the sustained activation of the MAP kinase cascade. Our data also emphasize the complex regulatory network underlying three interconnected processes in P. anserina: sexual reproduction, defence against competitors, and cell degeneration.
Philippe Silar - One of the best experts on this subject based on the ideXlab platform.
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Appressorium: The Breakthrough in Dikarya.
Journal of fungi (Basel Switzerland), 2019Co-Authors: Alexander Demoor, Philippe Silar, Sylvain BrunAbstract:Phytopathogenic and mycorrhizal fungi often penetrate living hosts by using appressoria and related structures. The differentiation of similar structures in saprotrophic fungi to penetrate dead plant biomass has seldom been investigated and has been reported only in the model fungus Podospora anserina. Here, we report on the ability of many saprotrophs from a large range of taxa to produce appressoria on cellophane. Most Ascomycota and Basidiomycota were able to form appressoria. In contrast, none of the three investigated Mucoromycotina was able to differentiate such structures. The ability of filamentous fungi to differentiate appressoria no longer belongs solely to pathogenic or mutualistic fungi, and this raises the question of the evolutionary origin of the appressorium in Eumycetes.
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A gene graveyard in the genome of the fungus Podospora comata
Molecular Genetics and Genomics, 2019Co-Authors: Philippe Silar, Valérie Gautier, Pierre Grognet, Arnaud Couloux, Jean-marc Dauget, Michelle Chablat, Sylvie Hermann-le Denmat, Patrick Wincker, Robert DebuchyAbstract:Mechanisms involved in fine adaptation of fungi to their environment include differential gene regulation associated with single nucleotide polymorphisms and indels (including transposons), horizontal gene transfer, gene copy amplification, as well as pseudogenization and gene loss. The two Podospora genome sequences examined here emphasize the role of pseudogenization and gene loss, which have rarely been documented in fungi. Podospora comata is a species closely related to Podospora anserina, a fungus used as model in several laboratories. Comparison of the genome of P. comata with that of P. anserina, whose genome is available for over 10 years, should yield interesting data related to the modalities of genome evolution between these two closely related fungal species that thrive in the same types of biotopes, i.e., herbivore dung. Here, we present the genome sequence of the mat+isolate of the P. comata reference strain T. Comparison with the genome of the mat+isolate of P. anserina strain S confirms that P. anserina and P. comata are likely two different species that rarely interbreed in nature. Despite having a 94-99% of nucleotide identity in the syntenic regions of their genomes, the two species differ by nearly 10% of their gene contents. Comparison of the species-specific gene sets uncovered genes that could be responsible for the known physiological differences between the two species. Finally, we identified 428 and 811 pseudogenes (3.8 and 7.2% of the genes) in P. anserina and P. comata, respectively. Presence of high numbers of pseudogenes supports the notion that difference in gene contents is due to gene loss rather than horizontal gene transfers. We propose that the high frequency of pseudogenization leading to gene loss in P. anserina and P. comata accompanies specialization of these two fungi. Gene loss may be more prevalent during the evolution of other fungi than usually thought.
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Plant biomass degrading ability of the coprophilic ascomycete fungus Podospora anserina.
Biotechnology advances, 2016Co-Authors: Marie Couturier, Philippe Silar, Sylvain Brun, Narumon Tangthirasunun, Valérie Gautier, Xie Ning, Chloé Bennati-granier, Jean-guy BerrinAbstract:The degradation of plant biomass is a major challenge towards the production of bio-based compounds and materials. As key lignocellulolytic enzyme producers, filamentous fungi represent a promising reservoir to tackle this challenge. Among them, the coprophilous ascomycete Podospora anserina has been used as a model organism to study various biological mechanisms because its genetics are well understood and controlled. In 2008, the sequencing of its genome revealed a great diversity of enzymes targeting plant carbohydrates and lignin. Since then, a large array of lignocellulose-acting enzymes has been characterized and genetic analyses have enabled the understanding of P. anserina metabolism and development on plant biomass. Overall, these research efforts shed light on P. anserina strategy to unlock recalcitrant lignocellulose deconstruction.
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Inactivation of cellobiose dehydrogenases modifies the cellulose degradation mechanism of Podospora anserina
Applied and Environmental Microbiology, 2016Co-Authors: Narumon Tangthirasunun, Philippe Silar, David Navarro, Sona Garajova, Didier Chevret, Laetitia Chan Ho Tong, Valérie Gautier, Kevin D. Hyde, Jean-guy BerrinAbstract:Conversion of biomass into high-value products, including biofuels, is of great interest to developing sustainable biorefineries. Fungi are an inexhaustible source of enzymes to degrade plant biomass. Cellobiose dehydrogenases (CDHs) play an important role in the breakdown through synergistic action with fungal lytic polysaccharide monooxygenases (LPMOs). The three CDH genes of the model fungus Podospora anserina were inactivated, resulting in single and multiple CDH mutants. We detected almost no difference in growth and fertility of the mutants on various lignocellulose sources, except on crystalline cellulose, on which a 2-fold decrease in fertility of the mutants lacking P. anserina CDH1 (PaCDH1) and PaCDH2 was observed. A striking difference between wild-type and mutant secretomes was observed. The secretome of the mutant lacking all CDHs contained five beta-glucosidases, whereas the wild type had only one. P. anserina seems to compensate for the lack of CDH with secretion of beta-glucosidases. The addition of P. anserina LPMO to either the wild-type or mutant secretome resulted in improvement of cellulose degradation in both cases, suggesting that other redox partners present in the mutant secretome provided electrons to LPMOs. Overall, the data showed that oxidative degradation of cellulosic biomass relies on different types of mechanisms in fungi.IMPORTANCE:Plant biomass degradation by fungi is a complex process involving dozens of enzymes. The roles of each enzyme or enzyme class are not fully understood, and utilization of a model amenable to genetic analysis should increase the comprehension of how fungi cope with highly recalcitrant biomass. Here, we report that the cellobiose dehydrogenases of the model fungus Podospora anserina enable it to consume crystalline cellulose yet seem to play a minor role on actual substrates, such as wood shavings or miscanthus. Analysis of secreted proteins suggests that Podospora anserina compensates for the lack of cellobiose dehydrogenase by increasing beta-glucosidase expression and using an alternate electron donor for LPMO.
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Podospora anserina: From Laboratory to Biotechnology
Soil Biology, 2013Co-Authors: Philippe SilarAbstract:Although not as popular as its relative Neurospora crassa or its more distant cousin Aspergillus nidulans, Podospora anserina has much to offer as a laboratory model. Indeed, this non-pathogenic species, easy to grow and reproduce sexually, has been used for nearly 100 years to study various phenomena of general importance in biology such as sexual reproduction, cell differentiation and death, prions and prion-like infectious factors, protein translation, mitochondrial physiology or ageing. With the availability of the complete genome sequence, new technologies are being developed, which, combined with the fast and efficient genetic analysis possible with P. anserina, make studies with this organism even more effective. Moreover, the genome sequence has enabled access to hitherto unknown genes involved in the adaptation of P. anserina to its biotope and with potential application in biomass degradation for the biofuel industries, in bioremediation of polluted soils and in production of secondary metabolites with interesting activities. Without doubt additional important discoveries are to be made by studying this organism and will permit an even broader usage of P. anserina in biotechnology.
Annie Sainsard-chanet - One of the best experts on this subject based on the ideXlab platform.
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Two nuclear life-cycle-regulated genes encode interchangeable subunits c of mitochondrial ATP synthase in Podospora anserina.
Molecular Biology and Evolution, 2011Co-Authors: Michelle Déquard-chablat, Annie Sainsard-chanet, Carole H. Sellem, Sylvie Hermann-le Denmat, Pawel Golik, Frédérique Bidard, Alexandre Martos, Maïlis Bietenhader, Jean-paul Di Rago, Véronique ContamineAbstract:An F(1)F(O) ATP synthase in the inner mitochondrial membrane catalyzes the late steps of ATP production via the process of oxidative phosphorylation. A small protein subunit (subunit c or ATP9) of this enzyme shows a substantial genetic diversity, and its gene can be found in both the mitochondrion and/or nucleus. In a representative set of 26 species of Fungi for which the genomes have been entirely sequenced we found five Atp9 gene repartitions. The phylogenetic distribution of nuclear and mitochondrial Atp9 genes suggests that their evolution has included two independent transfers to the nucleus followed by several independent episodes of the loss of the mitochondrial and/or nuclear gene. Interestingly, we found that in Podospora anserina, subunit c is exclusively produced from two nuclear genes (PaAtp9-5 and PaAtp9-7), which display different expression profiles through the life cycle of the fungus. The PaAtp9-5 gene is specifically and strongly expressed in germinating ascospores whereas PaAtp9-7 is mostly transcribed during sexual reproduction. Consistent with these observations, deletion of PaAtp9-5 is lethal whereas PaAtp9-7 deletion strongly impairs ascospore production. The P. anserina PaAtp9-5 and PaAtp9-7 genes are therefore non-redundant. By swapping the 5' and 3' flanking regions between genes we demonstrated, however, that the PaAtp9 coding sequences are functionally interchangeable. These findings show that after transfer to the nucleus, the subunit c gene in Podospora became a key target for the modulation of cellular energy metabolism according to the requirements of the life cycle.
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gene deletion and allelic replacement in the filamentous fungus Podospora anserina
Current Genetics, 2008Co-Authors: Riyad El-khoury, Marc F.p.m. Maas, Carole H. Sellem, Evelyne Coppin, Antoine Boivin, Robert Debuchy, Annie Sainsard-chanetAbstract:Gene replacement via homologous recombination is a fundamental tool for the analysis of gene function. However, this event is rare in organisms like the filamentous fungus Podospora anserina. We show here that deletion of the PaKu70 gene is an efficient strategy for improving gene manipulation in this organism. By using the ΔPaKu70 strain, it is now possible (1) to produce deletion mutants with an efficiency of 100%, (2) to achieve allelic exchange by introducing a mutated allele associated with a selection cassette at the locus, (3) to introduce a mutation in a gene without co-insertion of a selectable marker and without any modification of the target locus.
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Integration of a pAL2-1 homologous mitochondrial plasmid associated with life span extension in Podospora anserina.
Fungal Genetics and Biology, 2007Co-Authors: M. F. P. M. Maas, Carole H. Sellem, R. F. Hoekstra, A. J. M. Debets, Annie Sainsard-chanetAbstract:We isolated and characterized a novel spontaneous longevity mutant of Podospora anserina strain Wa32 carrying one of the pAL2-1 homologous mitochondrial plasmids. This mutant is at least ten fold longer-lived than the wild type, and is hence a formal suppressor of both the regular and the 'plasmid-based' senescence process. We show that the longevity trait is maternally inherited and coincides with the presence of a copy of the plasmid integrated in the 5' UTR of the mitochondrial Complex I genes nd2 and nd3. This mutation is associated with complex alterations in the respiratory chain, including a dispensable induction of the alternative oxidase. It is also associated with a stabilization of the mitochondrial chromosome and a reduction of the overall cellular level of reactive oxygen species.
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mitochondrial metabolism and aging in the filamentous fungus Podospora anserina
Biochimica et Biophysica Acta, 2006Co-Authors: Séverine Lorin, Eric Dufour, Annie Sainsard-chanetAbstract:The filamentous fungus Podospora anserina has a limited lifespan. In this organism, aging is systematically associated to mitochondrial DNA instability. We recently provided evidence that the respiratory function is a key determinant of its lifespan. Loss of function of the cytochrome pathway leads to the compensatory induction of an alternative oxidase, to a decreased production of reactive oxygen species and to a striking increase in lifespan. These changes are associated to the stabilization of the mitochondrial DNA. Here we review and discuss the links between these different parameters and their implication in the control of lifespan. Since we demonstrated the central role of mitochondrial metabolism in aging, the same relationship has been evidenced in several model systems from yeast to mice, confirming the usefulness of simple organisms as P. anserina for studying lifespan regulation.
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A causal link between respiration and senescence in Podospora anserina
Proceedings of the National Academy of Sciences of the United States of America, 2000Co-Authors: Eric Dufour, Joceline Boulay, Vincent Rincheval, Annie Sainsard-chanetAbstract:Senescence, a progressive degenerative process leading to age-related increase in mortality, is found in most eukaryotes. However, the molecular events underlying aging remain largely unknown. Understanding how longevity is regulated is a fundamental problem. Here we demonstrate that the respiratory function is a key factor that contributes to shortening lifespan of the filamentous fungus Podospora anserina. In this organism, senescence is systematically associated with mitochondrial DNA instabilities. We show that inactivation of the nuclear COX5 gene encoding subunit V of the cytochrome c oxidase complex leads to the exclusive use of the alternative respiratory pathway and to a decrease in production of reactive oxygen species. This inactivation results in a striking increase of longevity associated with stabilization of the mitochondrial chromosome. Moreover, accumulation of several senescence-specific mitochondrial DNA molecules is prevented in this nuclear mutant. These findings provide direct evidence of a causal link between mitochondrial metabolism and longevity in Podospora anserina.
Heinz D Osiewacz - One of the best experts on this subject based on the ideXlab platform.
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correction a genome wide longitudinal transcriptome analysis of the aging model Podospora anserina
PLOS ONE, 2013Co-Authors: Oliver Philipp, Andrea Hamann, Jorg Servos, Alexandra Werner, Ina Koch, Heinz D OsiewaczAbstract:The species name "Podospora anserina" is spelled incorrectly in the article title. The correct title is: "A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserina." The correct Citation is: Philipp O, Hamann A, Servos J, Werner A, Koch I, et al. (2013) A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserina. PLoS ONE 8(12): e83109. doi:10.1371/journal.pone.0083109
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human clpp reverts the longevity phenotype of a fungal clpp deletion strain
Nature Communications, 2013Co-Authors: Fabian Fischer, Andrea Hamann, Andrea Weil, Heinz D OsiewaczAbstract:Mitochondrial maintenance crucially depends on the quality control of proteins by various chaperones, proteases and repair enzymes. While most of the involved components have been studied in some detail, little is known on the biological role of the CLPXP protease complex located in the mitochondrial matrix. Here we show that deletion of PaClpP, encoding the CLP protease proteolytic subunit CLPP, leads to an unexpected healthy phenotype and increased lifespan of the fungal ageing model organism Podospora anserina. This phenotype can be reverted by expression of human ClpP in the fungal deletion background, demonstrating functional conservation of human and fungal CLPP. Our results show that the biological role of eukaryotic CLP proteases can be studied in an experimentally accessible model organism.
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assessing organismal aging in the filamentous fungus Podospora anserina
Methods of Molecular Biology, 2013Co-Authors: Heinz D Osiewacz, Andrea Hamann, Sandra ZintelAbstract:: Podospora anserina is an extensively studied model organism to unravel the mechanism of organismal aging. This filamentous fungus is short-lived and accessible to experimentation. Aging and lifespan are controlled by genetic and environmental traits and, in this model, have a strong mitochondrial etiology. Here, we describe methods and protocols to manipulate and study the aging process in P. anserina at different levels including biochemistry, cell biology, genetics, and physiology.
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Mitochondrial quality control in aging and lifespan control of the fungal aging model Podospora anserina
Biochemical Society transactions, 2011Co-Authors: Heinz D OsiewaczAbstract:Aging of biological systems is a fundamental process controlled by a complex network of molecular pathways. In the filamentous fungus Podospora anserina, a model in which organismal aging can conveniently be analysed, mitochondria play a central role. A wide range of relevant pathways were identified that contribute to the maintenance of a population of functional mitochondria. These pathways act in a hierarchical manner, but all the pathways are limited in capacity. At the end of the life cycle, when the various surveillance pathways are overwhelmed and damage has passed certain thresholds, programmed cell death brings the life of individual P. anserina to an end.
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the s adenosylmethionine dependent o methyltransferase pamth1 a longevity assurance factor protecting Podospora anserina against oxidative stress
Aging (Albany NY), 2009Co-Authors: Birgit Kunstmann, Heinz D OsiewaczAbstract:PaMTH1 is an O-methyltransferase catalysing the methylation of vicinal hydroxyl groups of polyphenols. The protein accumulates during ageing of Podospora anserina in both the cytosol and in the mitochondrial matrix. The construction and characterisation of a PaMth1 deletion strain provided additional evidence about the function of the protein in the protection against metal induced oxidative stress. Deletion of PaMth1 was found to lead to a decreased resistance against exogenous oxidative stress and to a shortened lifespan suggesting a role of PaMTH1 as a longevity assurance factor in a new molecular pathway involved in lifespan control.
