Potexvirus

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Armelle Marais - One of the best experts on this subject based on the ideXlab platform.

  • Two novel Alphaflexiviridae members revealed by deep sequencing of the Vanilla (Orchidaceae) virome
    Archives of Virology, 2017
    Co-Authors: Michel Grisoni, Anne Saison, Sandy Contreras, Stephan Theil, Armelle Marais, C Julian, Pierre-yves Teycheney, C Faure, Denis Filloux, Philippe Roumagnac
    Abstract:

    The genomes of two novel viruses were assembled from 454 pyrosequencing data obtained from vanilla leaves from La Réunion. Based on genome organization and homologies, one agent was unambiguously classified as a member of the genus Potexvirus and named vanilla virus X (VVX). The second one, vanilla latent virus (VLV), is phylogenetically close to three unclassified members of the family Alphaflexiviridae with similarity to allexiviruses, and despite the presence of an additional 8-kDa open reading frame, we propose to include VLV as a new member of the genus Allexivirus. Both VVX and VLV were mechanically transmitted to vanilla plants, resulting in asymptomatic infections.

  • Molecular characterization of yam virus X, a new Potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct Potexviruses infecting yams.
    Archives of Virology, 2014
    Co-Authors: Isabelle Acina Mambole, Denis Filloux, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Chantal Faure, David Lange, Laurence Svanella-dumas, Claude Pavis, Armelle Marais
    Abstract:

    The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infects members of the complex D. cayenensis rotundata. This study provides evidence for the existence of two additional Potexviruses, one of which infects D. nummularia in Vanuatu and the other, D. bulbifera and D. rotundata in Haiti and D. trifida and D. rotundata in Guadeloupe. This work also shows that existing Potexvirus-specific degenerate primers targeting the ORF1-encoded polymerase domain are well suited for the identification of the three Potexviruses reported here.

  • Characterization and diagnostic of Yam virus X (YVX) and Yam necrosis virus (YNV), two novel viruses infecting yams in Guadeloupe
    2013
    Co-Authors: Isabelle Nina Acina Manbole, Armelle Marais, Philippe Roumagnac, Claudie Pavis, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Dominique Lange, Chantal Faure, Denis Filloux
    Abstract:

    Several viral species infecting cultivated yams (Dioscorea spp.) are known. They include viruses belonging to the families Alphaflexiviridae (genus Potexvirus), Betaflexiviridae (genus Carlavirus), Caulimoviridae (genus Badnavirus), Cucumoviridae (genus Cucumovirus) and Potyviridae (genera Macluravirus and Potyvirus). However, it is widely acknowledged that yet uncharacterized viral species are present in yam germplasm collections worldwide and could be propagated through the distribution of infected germplasm. Therefore, viruses are currently the major constraint for much needed exchanges and distribution of yam germplasm. In order to promote the safe exchange of yam germplasm conserved in the Guadeloupe Biological Resources Center of Tropical Plants (CRB-PT), searches for new virus species in yams were undertaken. In silico analyses of ESTs of Dioscorea alata were performed and unveiled the existence of sequences corresponding to several known genera of yam viruses, such as Badnavirus and Macluravirus, and also to families of unknown yam-associated viruses, including Geminiviridae and Secoviridae. RT-PCR were performed on crude extracts of symptomatic yams (D. alata, D. trifida) following direct binding of viral particles and using degenerate primers. Amplification products were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and with viruses of the family Secoviridae. The 3' ends of the corresponding viral genomes were successfully amplified by 3' RACE, cloned and sequenced. Phylogenetic analyses confirmed the existence in yams of one new viral species within the genus Potexvirus (tentatively named Yam virus X, YVX) and one within the family Secoviridae (tentatively named Yam necrosis virus, YNV). The experimental host range of both viruses was explored through mechanical inoculation on various herbaceous plants. Molecular diagnostic was developed for both YVX and YNV using direct binding reverse transcription PCR (DB-RT-PCR) and used to perform a prevalence study of both viruses in the Guadeloupe CRB-PT yam germplasm collection. (Texte integral)

  • Caractérisation moléculaire du Yam virus X (YVX) et du Yam necrosis virus (YNV), deux nouveaux virus des genres Potexvirus et Sadwavirus infectant les ignames en Guadeloupe, et mise au point d’outils de diagnostic
    2012
    Co-Authors: Isabelle Acina-mambole, Armelle Marais, Philippe Roumagnac, Denis Filloux, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Chantal Faure, David Lange, Thierry Candresse
    Abstract:

    Les ignames (Dioscorea spp.) sont des ressources alimentaires économiquement et socialement importantes en Afrique, dans la Caraïbe, en Amérique du Sud et dans les îles du Pacifique. Les espèces cultivées hébergent de nombreux virus des genres Potexvirus, carlavirus, badnavirus, cucumovirus, macluravirus et potyvirus, qui constituent la principale contrainte pour la conservation et la diffusion de ressources génétiques. Deux nouveaux virus appartenant aux genres Potexvirus et sadwavirus, baptisés respectivement Yam virus X (YVX) et Yam necrosis virus (YNV), ont été mis en évidence par analyses in silico d’EST d’ignames. La partie 3’ du génome de chacun de ces virus a été amplifiée par 3’RACE à partir de plantes infectées et présentant des symptômes de nécrose foliaire récoltées en Guadeloupe, clonée puis séquencée. Les séquences obtenues ont permis d’établir les relations phylogéniques de ces espèces virales au sein des genres Potexvirus et sadwavirus. Des amorces spécifiques de chacun de ces deux virus ont été dessinées dans la région codant l’ARN polymérase ARN dépendante (RdRp) et utilisées pour la mise au point de tests de détection moléculaire par direct binding reverse transcription PCR (DB-RT-PCR) dans des broyats de feuilles. Ces outils complètent l’arsenal disponible pour le diagnostic des virus des ignames et permettent d’entreprendre des études de prévalence du YVX et du YNV en Guadeloupe.

Denis Filloux - One of the best experts on this subject based on the ideXlab platform.

  • Two novel Alphaflexiviridae members revealed by deep sequencing of the Vanilla (Orchidaceae) virome
    Archives of Virology, 2017
    Co-Authors: Michel Grisoni, Anne Saison, Sandy Contreras, Stephan Theil, Armelle Marais, C Julian, Pierre-yves Teycheney, C Faure, Denis Filloux, Philippe Roumagnac
    Abstract:

    The genomes of two novel viruses were assembled from 454 pyrosequencing data obtained from vanilla leaves from La Réunion. Based on genome organization and homologies, one agent was unambiguously classified as a member of the genus Potexvirus and named vanilla virus X (VVX). The second one, vanilla latent virus (VLV), is phylogenetically close to three unclassified members of the family Alphaflexiviridae with similarity to allexiviruses, and despite the presence of an additional 8-kDa open reading frame, we propose to include VLV as a new member of the genus Allexivirus. Both VVX and VLV were mechanically transmitted to vanilla plants, resulting in asymptomatic infections.

  • Molecular characterization of yam virus X, a new Potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct Potexviruses infecting yams.
    Archives of Virology, 2014
    Co-Authors: Isabelle Acina Mambole, Denis Filloux, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Chantal Faure, David Lange, Laurence Svanella-dumas, Claude Pavis, Armelle Marais
    Abstract:

    The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infects members of the complex D. cayenensis rotundata. This study provides evidence for the existence of two additional Potexviruses, one of which infects D. nummularia in Vanuatu and the other, D. bulbifera and D. rotundata in Haiti and D. trifida and D. rotundata in Guadeloupe. This work also shows that existing Potexvirus-specific degenerate primers targeting the ORF1-encoded polymerase domain are well suited for the identification of the three Potexviruses reported here.

  • Characterization and diagnostic of Yam virus X (YVX) and Yam necrosis virus (YNV), two novel viruses infecting yams in Guadeloupe
    2013
    Co-Authors: Isabelle Nina Acina Manbole, Armelle Marais, Philippe Roumagnac, Claudie Pavis, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Dominique Lange, Chantal Faure, Denis Filloux
    Abstract:

    Several viral species infecting cultivated yams (Dioscorea spp.) are known. They include viruses belonging to the families Alphaflexiviridae (genus Potexvirus), Betaflexiviridae (genus Carlavirus), Caulimoviridae (genus Badnavirus), Cucumoviridae (genus Cucumovirus) and Potyviridae (genera Macluravirus and Potyvirus). However, it is widely acknowledged that yet uncharacterized viral species are present in yam germplasm collections worldwide and could be propagated through the distribution of infected germplasm. Therefore, viruses are currently the major constraint for much needed exchanges and distribution of yam germplasm. In order to promote the safe exchange of yam germplasm conserved in the Guadeloupe Biological Resources Center of Tropical Plants (CRB-PT), searches for new virus species in yams were undertaken. In silico analyses of ESTs of Dioscorea alata were performed and unveiled the existence of sequences corresponding to several known genera of yam viruses, such as Badnavirus and Macluravirus, and also to families of unknown yam-associated viruses, including Geminiviridae and Secoviridae. RT-PCR were performed on crude extracts of symptomatic yams (D. alata, D. trifida) following direct binding of viral particles and using degenerate primers. Amplification products were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and with viruses of the family Secoviridae. The 3' ends of the corresponding viral genomes were successfully amplified by 3' RACE, cloned and sequenced. Phylogenetic analyses confirmed the existence in yams of one new viral species within the genus Potexvirus (tentatively named Yam virus X, YVX) and one within the family Secoviridae (tentatively named Yam necrosis virus, YNV). The experimental host range of both viruses was explored through mechanical inoculation on various herbaceous plants. Molecular diagnostic was developed for both YVX and YNV using direct binding reverse transcription PCR (DB-RT-PCR) and used to perform a prevalence study of both viruses in the Guadeloupe CRB-PT yam germplasm collection. (Texte integral)

  • Caractérisation moléculaire du Yam virus X (YVX) et du Yam necrosis virus (YNV), deux nouveaux virus des genres Potexvirus et Sadwavirus infectant les ignames en Guadeloupe, et mise au point d’outils de diagnostic
    2012
    Co-Authors: Isabelle Acina-mambole, Armelle Marais, Philippe Roumagnac, Denis Filloux, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Chantal Faure, David Lange, Thierry Candresse
    Abstract:

    Les ignames (Dioscorea spp.) sont des ressources alimentaires économiquement et socialement importantes en Afrique, dans la Caraïbe, en Amérique du Sud et dans les îles du Pacifique. Les espèces cultivées hébergent de nombreux virus des genres Potexvirus, carlavirus, badnavirus, cucumovirus, macluravirus et potyvirus, qui constituent la principale contrainte pour la conservation et la diffusion de ressources génétiques. Deux nouveaux virus appartenant aux genres Potexvirus et sadwavirus, baptisés respectivement Yam virus X (YVX) et Yam necrosis virus (YNV), ont été mis en évidence par analyses in silico d’EST d’ignames. La partie 3’ du génome de chacun de ces virus a été amplifiée par 3’RACE à partir de plantes infectées et présentant des symptômes de nécrose foliaire récoltées en Guadeloupe, clonée puis séquencée. Les séquences obtenues ont permis d’établir les relations phylogéniques de ces espèces virales au sein des genres Potexvirus et sadwavirus. Des amorces spécifiques de chacun de ces deux virus ont été dessinées dans la région codant l’ARN polymérase ARN dépendante (RdRp) et utilisées pour la mise au point de tests de détection moléculaire par direct binding reverse transcription PCR (DB-RT-PCR) dans des broyats de feuilles. Ces outils complètent l’arsenal disponible pour le diagnostic des virus des ignames et permettent d’entreprendre des études de prévalence du YVX et du YNV en Guadeloupe.

  • Molecular characterization of a sadwavirus and a Potexvirus infecting yams (Dioscorea spp) in Guadeloupe
    2011
    Co-Authors: Fabiola Anzala, Philippe Roumagnac, Denis Filloux, Rose-marie Gomez, Thierry Candresse, Claude Pavis, Pierre-yves Teycheney
    Abstract:

    Yams (Dioscorea sp.) are important food commodities in tropical and subtropical areas of Africa, the Caribbean, South America and the Pacific, where they play a key role in food security. Being vegetatively propagated crops, yams cannot be sanitized via seed production and therefore accumulate viruses over long periods of time. Potexvirus, Carlavirus, Badnavirus, Cucumovirus, Macluravirus and Potyvirus species have been characterized in yams. In addition ESTs (Expressed Sequence Tags) of Dioscorea alata include sequences from families of viruses yet unknown to infect yams, such as Geminiviridae and Secoviridae, showing that the diversity of viral species infecting this crop remains underestimated. This situation has prompted a search for yet uncharacterized viruses infecting yams in Guadeloupe (French West Indies). RT-PCR was performed on crude extracts of symptomatic yams (D. alata, D. trifida), following direct binding of viral particles and using Potexvirus degenerate primers [1]. Several samples gave amplification products, which were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and Sadwaviruses. The 3’ ends of the corresponding viral genomes have been amplified by 3’ RACE, cloned and sequenced. Phylogenetic analyses confirm the existence in yams of a new viral species within the genus Potexvirus, tentatively named Yam virus X (YVX), and of a new viral species within the genus Sadwavirus, tentatively named Yam necrosis virus (YNV).

Philippe Roumagnac - One of the best experts on this subject based on the ideXlab platform.

  • Two novel Alphaflexiviridae members revealed by deep sequencing of the Vanilla (Orchidaceae) virome
    Archives of Virology, 2017
    Co-Authors: Michel Grisoni, Anne Saison, Sandy Contreras, Stephan Theil, Armelle Marais, C Julian, Pierre-yves Teycheney, C Faure, Denis Filloux, Philippe Roumagnac
    Abstract:

    The genomes of two novel viruses were assembled from 454 pyrosequencing data obtained from vanilla leaves from La Réunion. Based on genome organization and homologies, one agent was unambiguously classified as a member of the genus Potexvirus and named vanilla virus X (VVX). The second one, vanilla latent virus (VLV), is phylogenetically close to three unclassified members of the family Alphaflexiviridae with similarity to allexiviruses, and despite the presence of an additional 8-kDa open reading frame, we propose to include VLV as a new member of the genus Allexivirus. Both VVX and VLV were mechanically transmitted to vanilla plants, resulting in asymptomatic infections.

  • Characterization and diagnostic of Yam virus X (YVX) and Yam necrosis virus (YNV), two novel viruses infecting yams in Guadeloupe
    2013
    Co-Authors: Isabelle Nina Acina Manbole, Armelle Marais, Philippe Roumagnac, Claudie Pavis, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Dominique Lange, Chantal Faure, Denis Filloux
    Abstract:

    Several viral species infecting cultivated yams (Dioscorea spp.) are known. They include viruses belonging to the families Alphaflexiviridae (genus Potexvirus), Betaflexiviridae (genus Carlavirus), Caulimoviridae (genus Badnavirus), Cucumoviridae (genus Cucumovirus) and Potyviridae (genera Macluravirus and Potyvirus). However, it is widely acknowledged that yet uncharacterized viral species are present in yam germplasm collections worldwide and could be propagated through the distribution of infected germplasm. Therefore, viruses are currently the major constraint for much needed exchanges and distribution of yam germplasm. In order to promote the safe exchange of yam germplasm conserved in the Guadeloupe Biological Resources Center of Tropical Plants (CRB-PT), searches for new virus species in yams were undertaken. In silico analyses of ESTs of Dioscorea alata were performed and unveiled the existence of sequences corresponding to several known genera of yam viruses, such as Badnavirus and Macluravirus, and also to families of unknown yam-associated viruses, including Geminiviridae and Secoviridae. RT-PCR were performed on crude extracts of symptomatic yams (D. alata, D. trifida) following direct binding of viral particles and using degenerate primers. Amplification products were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and with viruses of the family Secoviridae. The 3' ends of the corresponding viral genomes were successfully amplified by 3' RACE, cloned and sequenced. Phylogenetic analyses confirmed the existence in yams of one new viral species within the genus Potexvirus (tentatively named Yam virus X, YVX) and one within the family Secoviridae (tentatively named Yam necrosis virus, YNV). The experimental host range of both viruses was explored through mechanical inoculation on various herbaceous plants. Molecular diagnostic was developed for both YVX and YNV using direct binding reverse transcription PCR (DB-RT-PCR) and used to perform a prevalence study of both viruses in the Guadeloupe CRB-PT yam germplasm collection. (Texte integral)

  • Caractérisation moléculaire du Yam virus X (YVX) et du Yam necrosis virus (YNV), deux nouveaux virus des genres Potexvirus et Sadwavirus infectant les ignames en Guadeloupe, et mise au point d’outils de diagnostic
    2012
    Co-Authors: Isabelle Acina-mambole, Armelle Marais, Philippe Roumagnac, Denis Filloux, Rose-marie Gomez, Lydiane Bonheur, Fabiola Anzala, Chantal Faure, David Lange, Thierry Candresse
    Abstract:

    Les ignames (Dioscorea spp.) sont des ressources alimentaires économiquement et socialement importantes en Afrique, dans la Caraïbe, en Amérique du Sud et dans les îles du Pacifique. Les espèces cultivées hébergent de nombreux virus des genres Potexvirus, carlavirus, badnavirus, cucumovirus, macluravirus et potyvirus, qui constituent la principale contrainte pour la conservation et la diffusion de ressources génétiques. Deux nouveaux virus appartenant aux genres Potexvirus et sadwavirus, baptisés respectivement Yam virus X (YVX) et Yam necrosis virus (YNV), ont été mis en évidence par analyses in silico d’EST d’ignames. La partie 3’ du génome de chacun de ces virus a été amplifiée par 3’RACE à partir de plantes infectées et présentant des symptômes de nécrose foliaire récoltées en Guadeloupe, clonée puis séquencée. Les séquences obtenues ont permis d’établir les relations phylogéniques de ces espèces virales au sein des genres Potexvirus et sadwavirus. Des amorces spécifiques de chacun de ces deux virus ont été dessinées dans la région codant l’ARN polymérase ARN dépendante (RdRp) et utilisées pour la mise au point de tests de détection moléculaire par direct binding reverse transcription PCR (DB-RT-PCR) dans des broyats de feuilles. Ces outils complètent l’arsenal disponible pour le diagnostic des virus des ignames et permettent d’entreprendre des études de prévalence du YVX et du YNV en Guadeloupe.

  • Molecular characterization of a sadwavirus and a Potexvirus infecting yams (Dioscorea spp) in Guadeloupe
    2011
    Co-Authors: Fabiola Anzala, Philippe Roumagnac, Denis Filloux, Rose-marie Gomez, Thierry Candresse, Claude Pavis, Pierre-yves Teycheney
    Abstract:

    Yams (Dioscorea sp.) are important food commodities in tropical and subtropical areas of Africa, the Caribbean, South America and the Pacific, where they play a key role in food security. Being vegetatively propagated crops, yams cannot be sanitized via seed production and therefore accumulate viruses over long periods of time. Potexvirus, Carlavirus, Badnavirus, Cucumovirus, Macluravirus and Potyvirus species have been characterized in yams. In addition ESTs (Expressed Sequence Tags) of Dioscorea alata include sequences from families of viruses yet unknown to infect yams, such as Geminiviridae and Secoviridae, showing that the diversity of viral species infecting this crop remains underestimated. This situation has prompted a search for yet uncharacterized viruses infecting yams in Guadeloupe (French West Indies). RT-PCR was performed on crude extracts of symptomatic yams (D. alata, D. trifida), following direct binding of viral particles and using Potexvirus degenerate primers [1]. Several samples gave amplification products, which were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and Sadwaviruses. The 3’ ends of the corresponding viral genomes have been amplified by 3’ RACE, cloned and sequenced. Phylogenetic analyses confirm the existence in yams of a new viral species within the genus Potexvirus, tentatively named Yam virus X (YVX), and of a new viral species within the genus Sadwavirus, tentatively named Yam necrosis virus (YNV).

  • Molecular characterization of Secoviridae and Potexvirus infecting yams (Dioscorea spp)
    2011
    Co-Authors: Fabiola Anzala, Philippe Roumagnac, Denis Filloux, Claudie Pavis, Rose-marie Gomez, Pierre-yves Teycheney
    Abstract:

    The prevalence of virus species is generally high among vegetatively?propagated crops, because they cannot be sanitized via seed production and therefore accumulate viruses over long periods of time. In yams (Dioscorea spp), viruses belonging to the families Alphaflexiviridae (genus Potexvirus), Betaflexiviridae (genus carlavirus), Caulimoviridae (genus badnavirus), Cucumoviridae (genus cucumovirus) and Potyviridae (genera macluravirus and potyvirus) have been characterized so far. However, it is likely that the diversity of viral species infecting this crop remains underestimated. To test this hypothesis, in silico analyses of ESTs of Dioscorea alata were performed and unveiled the existence of sequences corresponding to several known genera of yam viruses, such as badnavirus and macluravirus, and also to families of unknown yam?associated viruses, including Geminiviridae and Secoviridae. This result has prompted a search for yet uncharacterized viruses infecting yams. RT?PCR performed on crude extracts of symptomatic yams (D. alata, D. trifida) following direct binding of viral particles and using available degenerate primers raised distinct amplification products, which were cloned and sequenced. Some of them displayed significant levels of homologies with Potexviruses and with viruses of the family Secoviridae. The 3' end of their genomes was successfully amplified by 3' RACE, cloned and sequenced. Sequence analyses and phylogenetic analyses confirmed the existence in yams of new viral species within the genus Potexvirus and the family Secoviridae. These results highlight the need to improve the current taxonomy of yam Potexviruses and suggest to further explore the diversity of viruses of the family Secoviridae in yams. (Texte integral)

Na-sheng Lin - One of the best experts on this subject based on the ideXlab platform.

  • molecular biology of bamboo mosaic virus a type member of the Potexvirus genus
    2020
    Co-Authors: Yau-heiu Hsu, Ching Hsiu Tsai, Na-sheng Lin
    Abstract:

    The flexible filamentous plant viruses are responsible for more than half of all agricultural loss worldwide. Potexvirus is one of the two most important flexible filamentous plant viruses. Bamboo mosaic virus (BaMV), a single-stranded positive-sense RNA virus, is a member of the Potexvirus genus of Alphaflexiviridae. It can infect at least 12 species of bamboo, causing a huge economic impact on the bamboo industry in Taiwan. The study of BaMV did not start extensively until the completion of the full-length sequencing of genomic RNA of BaMV and generation of the BaMV infectious cDNA clone in the early 1990s. Since then, BaMV has been extensively studied at the molecular, cellular and ecological level, covering both basic and applied researches, by a group of researchers in Taiwan. In this eBook, the content comprises 6 reviews and 4 articles. Seven of them are involved in the infection of BaMV covering viral RNA replication, viral RNA trafficking, and the host factors. Two of them are related to the vector transmission and the ecology of BaMV. The last one is the application of using BaMV as a viral vector to produce vaccines in plants.

  • Transmission of Bamboo mosaic virus in Bamboos Mediated by Insects in the Order Diptera
    Frontiers Media S.A., 2017
    Co-Authors: Kuo-chen Chang, Na-sheng Lin, Ling-teng Chang, Ying-wen Huang, Yi-chin Lai, Chin-wei Lee, Jia-teh Liao, Yau-heiu Hsu
    Abstract:

    Bamboo mosaic virus (BaMV), a member of the genus Potexvirus, is the major threat to bamboo cultivation. Similar to most Potexviruses, the transmission of BaMV by insect vectors has not been documented previously. However, field observations of BaMV disease incidences suggested that insect vectors might be involved. In this study, we aimed to investigate the possibility of insect-mediated transmission of BaMV among bamboo clumps, in order to provide further insights into the infection cycles of BaMV for the development of effective disease management measures. From the major insects collected from infected bamboo plantations, BaMV genomic RNAs were detected inside the bodies of two dipteran insects, Gastrozona fasciventris and Atherigona orientalis, but not in thrips (Scirtothrips dorsalis). Artificial feeding assays using green fluorescent protein-tagged BaMV virions revealed that BaMV could enter the digestive systems and survive in the regurgitant and excretion of the dipterans. BaMV RNA could be retained in the dipterans for up to 4 weeks. Insect-mediated transmission assays indicated that both dipterans could transmit BaMV to bamboo seedlings through artificially created wounds with low infection efficiency (14 – 41%), suggesting that the dipterans may mediate the transmission in a mechanical-like manner. These results demonstrated that dipterans with sponge-like mouthparts may also serve as vectors for at least one Potexvirus, BaMV, among bamboo plants. The finding suggested that dipteran insect control should be integrated into the disease management measures against BaMV infections

  • Generation of Subgenomic RNA Directed by a Satellite RNA Associated with Bamboo Mosaic Potexvirus: Analyses of Potexvirus Subgenomic RNA Promoter
    Journal of virology, 2000
    Co-Authors: Yun-shien Lee, Yau-heiu Hsu, Na-sheng Lin
    Abstract:

    Satellite RNA of bamboo mosaic Potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic Potexvirus (BaMV) for replication and encapsidation. A full-length cDNA clone of satBaMV was used to examine the sequences required for the synthesis of Potexvirus subgenomic RNAs (sgRNAs). Subgenomic promoter-like sequences (SGPs), 107 nucleotides (nt) upstream from the capsid protein (CP) gene of BaMV-V, were inserted upstream of the start codon of the P20 gene of satBaMV. Insertion of SGPs gave rise to the synthesis of sgRNA of satBaMV in protoplasts of Nicotiana benthamiana and leaves of Chenopodium quinoa when coinoculated with BaMV-V genomic RNA. Moreover, both the satBaMV cassette and its sgRNA were encapsidated. From analysis of the SGPs by deletion mutation, we concluded that an SGP contains one core promoterlike sequence (nt -30 through +16), two upstream enhancers (nt -59 through -31 and -91 through -60), and one downstream enhancer (nt +17 through +52), when the transcription initiation site is taken as +1. Site-directed mutagenesis and compensatory mutation to disrupt and restore potential base pairing in the core promoter-like sequence suggest that the stem-loop structure is important for the function of SGP in vivo. Likewise, the insertion of a putative SGP of the BaMV open reading frame 2 gene or a heterologous SGP of potato virus X resulted in generation of an sgRNA. The satBaMV cassette should be a useful tool to gain insight into sequences required for the synthesis of Potexvirus sgRNAs.

  • A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in Potexviruses.
    Virus genes, 1999
    Co-Authors: Ting-yu Yeh, Biing-yuan Lin, Ya-chun Chang, Yau-heiu Hsu, Na-sheng Lin
    Abstract:

    A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic Potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5′ terminus and 148 nts of the 3′ terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the Potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5′ and 3′ junction borders of reported Potexvirus D RNAs reveal a 65.2–84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of Potexvirus D RNAs.

  • Satellite RNA associated with bamboo mosaic Potexvirus shares similarity with satellites associated with sobemoviruses
    Archives of virology, 1995
    Co-Authors: J. S. Liu, Na-sheng Lin
    Abstract:

    A putative nonstructural protein encoded by a satellite RNA associated with bamboo mosaic Potexvirus shares 46% identity with the capsid protein of satellite virus of panicum mosaic sobemovirus. The sequence similarity among satellite plant viruses which have no apparent relationship implies a common origin.

Yau-heiu Hsu - One of the best experts on this subject based on the ideXlab platform.

  • molecular biology of bamboo mosaic virus a type member of the Potexvirus genus
    2020
    Co-Authors: Yau-heiu Hsu, Ching Hsiu Tsai, Na-sheng Lin
    Abstract:

    The flexible filamentous plant viruses are responsible for more than half of all agricultural loss worldwide. Potexvirus is one of the two most important flexible filamentous plant viruses. Bamboo mosaic virus (BaMV), a single-stranded positive-sense RNA virus, is a member of the Potexvirus genus of Alphaflexiviridae. It can infect at least 12 species of bamboo, causing a huge economic impact on the bamboo industry in Taiwan. The study of BaMV did not start extensively until the completion of the full-length sequencing of genomic RNA of BaMV and generation of the BaMV infectious cDNA clone in the early 1990s. Since then, BaMV has been extensively studied at the molecular, cellular and ecological level, covering both basic and applied researches, by a group of researchers in Taiwan. In this eBook, the content comprises 6 reviews and 4 articles. Seven of them are involved in the infection of BaMV covering viral RNA replication, viral RNA trafficking, and the host factors. Two of them are related to the vector transmission and the ecology of BaMV. The last one is the application of using BaMV as a viral vector to produce vaccines in plants.

  • Transmission of Bamboo mosaic virus in Bamboos Mediated by Insects in the Order Diptera
    Frontiers Media S.A., 2017
    Co-Authors: Kuo-chen Chang, Na-sheng Lin, Ling-teng Chang, Ying-wen Huang, Yi-chin Lai, Chin-wei Lee, Jia-teh Liao, Yau-heiu Hsu
    Abstract:

    Bamboo mosaic virus (BaMV), a member of the genus Potexvirus, is the major threat to bamboo cultivation. Similar to most Potexviruses, the transmission of BaMV by insect vectors has not been documented previously. However, field observations of BaMV disease incidences suggested that insect vectors might be involved. In this study, we aimed to investigate the possibility of insect-mediated transmission of BaMV among bamboo clumps, in order to provide further insights into the infection cycles of BaMV for the development of effective disease management measures. From the major insects collected from infected bamboo plantations, BaMV genomic RNAs were detected inside the bodies of two dipteran insects, Gastrozona fasciventris and Atherigona orientalis, but not in thrips (Scirtothrips dorsalis). Artificial feeding assays using green fluorescent protein-tagged BaMV virions revealed that BaMV could enter the digestive systems and survive in the regurgitant and excretion of the dipterans. BaMV RNA could be retained in the dipterans for up to 4 weeks. Insect-mediated transmission assays indicated that both dipterans could transmit BaMV to bamboo seedlings through artificially created wounds with low infection efficiency (14 – 41%), suggesting that the dipterans may mediate the transmission in a mechanical-like manner. These results demonstrated that dipterans with sponge-like mouthparts may also serve as vectors for at least one Potexvirus, BaMV, among bamboo plants. The finding suggested that dipteran insect control should be integrated into the disease management measures against BaMV infections

  • Generation of Subgenomic RNA Directed by a Satellite RNA Associated with Bamboo Mosaic Potexvirus: Analyses of Potexvirus Subgenomic RNA Promoter
    Journal of virology, 2000
    Co-Authors: Yun-shien Lee, Yau-heiu Hsu, Na-sheng Lin
    Abstract:

    Satellite RNA of bamboo mosaic Potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic Potexvirus (BaMV) for replication and encapsidation. A full-length cDNA clone of satBaMV was used to examine the sequences required for the synthesis of Potexvirus subgenomic RNAs (sgRNAs). Subgenomic promoter-like sequences (SGPs), 107 nucleotides (nt) upstream from the capsid protein (CP) gene of BaMV-V, were inserted upstream of the start codon of the P20 gene of satBaMV. Insertion of SGPs gave rise to the synthesis of sgRNA of satBaMV in protoplasts of Nicotiana benthamiana and leaves of Chenopodium quinoa when coinoculated with BaMV-V genomic RNA. Moreover, both the satBaMV cassette and its sgRNA were encapsidated. From analysis of the SGPs by deletion mutation, we concluded that an SGP contains one core promoterlike sequence (nt -30 through +16), two upstream enhancers (nt -59 through -31 and -91 through -60), and one downstream enhancer (nt +17 through +52), when the transcription initiation site is taken as +1. Site-directed mutagenesis and compensatory mutation to disrupt and restore potential base pairing in the core promoter-like sequence suggest that the stem-loop structure is important for the function of SGP in vivo. Likewise, the insertion of a putative SGP of the BaMV open reading frame 2 gene or a heterologous SGP of potato virus X resulted in generation of an sgRNA. The satBaMV cassette should be a useful tool to gain insight into sequences required for the synthesis of Potexvirus sgRNAs.

  • A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in Potexviruses.
    Virus genes, 1999
    Co-Authors: Ting-yu Yeh, Biing-yuan Lin, Ya-chun Chang, Yau-heiu Hsu, Na-sheng Lin
    Abstract:

    A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic Potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5′ terminus and 148 nts of the 3′ terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the Potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5′ and 3′ junction borders of reported Potexvirus D RNAs reveal a 65.2–84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of Potexvirus D RNAs.

  • Nucleotide sequence of the genomic RNA of bamboo mosaic Potexvirus
    Journal of General Virology, 1994
    Co-Authors: Na-sheng Lin, Biing-yuan Lin, Teh-yuan Chow, Yau-heiu Hsu
    Abstract:

    The complete nucleotide sequence of the genomic RNA of bamboo mosaic virus (BaMV) was determined by sequencing a set of overlapping cDNA clones and by direct sequencing of the viral RNA. The RNA genome of BaMV is 6366 nucleotides long [excluding 3′ poly(A) tail] and contains six open reading frames (ORFs 1 to 6) coding for polypeptides with M r values of 155K, 28K, 13K, 6K, 25K and 14K, respectively. The genome organization and sizes of the encoded proteins are very similar to those of other Potexviruses which have been sequenced except that ORF 6 lies completely within ORF 1. The first five putative proteins of the BaMV genome show identities ranging between 44 to 59%, 26 to 49%, 30 to 53%, 15 to 35% and 20 to 30%, respectively, to the corresponding ORFs of other members of the Potexvirus group. However the putative product ORF 6 shows no significant similarity to those of other Potexvirus ORF products.

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