The Experts below are selected from a list of 1742874 Experts worldwide ranked by ideXlab platform
Patricia Zambryski - One of the best experts on this subject based on the ideXlab platform.
-
Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.
Plant signaling & behavior, 2012Co-Authors: Tessa M. Burch-smith, Ya Cui, Patricia ZambryskiAbstract:Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated Proteins. Previously, overexpression of Arabidopsis C1RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of C1RGPs in Nicotiana benthamiana. Silenced plants show wild-type growth and development. Intercellular transport in silenced plants was probed using fluorescently labeled TMV and its movement Protein, P30. P30 shows increased cell-to-cell movement and TMV exhibited accelerated systemic spread compared with control plants. These results support the hypothesis that C1RGPs act to regulate intercellular transport via plasmodesmata.
-
Tobacco mosaic virus movement Protein associates with the cytoskeleton in tobacco cells.
The Plant cell, 1995Co-Authors: B G Mclean, J Zupan, Patricia ZambryskiAbstract:Tobacco mosaic virus movement Protein P30 complexes with genomic viral RNA for transport through plasmodesmata, the plant intercellular connections. Although most research with P30 focuses on its targeting to and gating of plasmodesmata, the mechanisms of P30 intracellular movement to plasmodesmata have not been defined. To examine P30 intracellular localization, we used tobacco protoplasts, which lack plasmodesmata, for transfection with plasmids carrying P30 coding sequences under a constitutive promoter and for infection with tobacco mosaic virus particles. In both systems, P30 appears as filaments that colocalize primarily with microtubules. To a lesser extent, P30 filaments colocalize with actin filaments, and in vitro experiments suggested that P30 can bind directly to actin and tubulin. This association of P30 with cytoskeletal elements may play a critical role in intracellular transport of the P30-viral RNA complex through the cytoplasm to and possibly through plasmodesmata.
Rajaneesh Anupam - One of the best experts on this subject based on the ideXlab platform.
-
Intrinsically Disordered Human T Lymphotropic Virus Type 1 P30 Protein: Experimental and Computational Evidence
AIDS research and human retroviruses, 2019Co-Authors: Priyanka Namdev, Denzelle Lee Lyngdoh, Hamid Y. Dar, Shivendra K. Chaurasiya, Rupesh K. Srivastava, Timir Tripathi, Rajaneesh AnupamAbstract:Abstract Human T lymphotropic virus type 1 (HTLV-1) causes adult T cell leukemia and lymphoma and other neuroinflammatory diseases. The pX region of HTLV-1 genome encodes an accessory Protein P30 t...
-
human t lymphotropic virus type 1 P30 interacts with regγ and modulates atm ataxia telangiectasia mutated to promote cell survival
Journal of Biological Chemistry, 2011Co-Authors: Rajaneesh Anupam, Antara Datta, Matthew Kesic, Kari B Greenchurch, Nikolozi Shkriabai, Mamuka Kvaratskhelia, Michael Dale LairmoreAbstract:Human T-lymphotropic virus type 1 (HTLV-1) is a causative agent of adult T cell leukemia/lymphoma and a variety of inflammatory disorders. HTLV-1 encodes a nuclear localizing Protein, P30, that selectively alters viral and cellular gene expression, activates G2-M cell cycle checkpoints, and is essential for viral spread. Here, we used immunoprecipitation and affinity pulldown of ectopically expressed P30 coupled with mass spectrometry to identify cellular binding partners of P30. Our data indicate that P30 specifically binds to cellular ATM (ataxia telangiectasia mutated) and REGγ (a nuclear 20 S proteasome activator). Under conditions of genotoxic stress, P30 expression was associated with reduced levels of ATM and increased cell survival. Knockdown or overexpression of REGγ paralleled P30 expression, suggesting an unexpected enhancement of P30 expression in the presence of REGγ. Finally, size exclusion chromatography revealed the presence of P30 in a high molecular mass complex along with ATM and REGγ. On the basis of our findings, we propose that HTLV-1 P30 interacts with ATM and REGγ to increase viral spread by facilitating cell survival.
Covadonga Alonso - One of the best experts on this subject based on the ideXlab platform.
-
African swine fever virus Protein P30 interaction with heterogeneous nuclear ribonucleoProtein K (hnRNP-K) during infection
FEBS Letters, 2008Co-Authors: Bruno Hernáez, José M. Escribano, Covadonga AlonsoAbstract:Heterogeneous nuclear ribonucleoProtein K (hnRNP-K) was identified as interacting cellular Protein with the abundant immediate early Protein P30 from African swine fever virus (ASFV) in a macrophage cDNA library screening. The interacting regions of hnRNP-K with P30 were established within residues 35–197, which represent KH1 and KH2 domains responsible for RNA binding. Colocalization of hnRNP-K and P30 was observed mainly in the nucleus, but not in the cytoplasm of infected cells and infection modified hnRNP-K subcellular distribution and decreased the incorporation of 5-fluorouridine into nascent RNA. Since similar effects were observed in cells transiently expressing P30, this interaction provides new insights into P30 function and could represent a possible additional mechanism by which ASFV downregulates host cell mRNA translation.
-
Serodiagnosis of African swine fever using the recombinant Protein P30 expressed in insect larvae.
Journal of Virological Methods, 2000Co-Authors: Maria G. Barderas, Andrés Wigdorovitz, F Merelo, Francisco J. Beitia, Covadonga Alonso, M.v. Borca, J.m. EscribanoAbstract:African swine fever (ASF) has a substantial economic impact in many African developing countries and its eradication is based only on an efficient diagnosis program because of the absence of an available vaccine. Previous data suggested the convenience of using the highly antigenic virus Protein P30 as ELISA antigen for serological diagnosis of this disease. A simple and efficient method is described for producing the recombinant Protein P30 from ASF virus in Trichoplusia ni larvae (cabbage looper) in order to facilitate the large-scale production of this recombinant Protein in the absence of fermentation procedures. A baculovirus encoding the virus Protein P30 was used to infect insect larvae, showing that recombinant Protein production had a sharp optimal peak with a time of occurrence dependent on the initial virus dose inoculated to the larvae. Crude lysates of infected larvae were used without further purification as coating antigen in ELISA to analyse a limited number of sera from natural or experimentally ASF virus infected pigs. Remarkably, the recombinant Protein obtained from a single infected larva was sufficient for serological diagnosis of at least 3750 serum samples. Recombinant P30 obtained by this procedure was also used in a confirmatory immunoblotting, reacting with all positive sera tested previously by ELISA. In conclusion, production of the recombinant ASF virus Protein P30 in larvae should be applicable to large-scale production of diagnostic reagents for this disease in developing countries, eliminating the need for specialised facilities for tissue culture.
-
Neutralizing antibodies to different Proteins of African swine fever virus inhibit both virus attachment and internalization.
Journal of virology, 1996Co-Authors: Paulino Gómez-puertas, Covadonga Alonso, Fernando Ramiro-ibáñez, Fernando Rodriguez, J.m. Oviedo, F. Ruiz-gonzalvo, J.m. EscribanoAbstract:African swine fever virus induces in convalescent pigs antibodies that neutralized the virus before and after binding to susceptible cells, inhibiting both virus attachment and internalization. A further analysis of the neutralization mechanisms mediated by the different viral Proteins showed that antibodies to Proteins p72 and p54 are involved in the inhibition of a first step of the replication cycle related to virus attachment, while antibodies to Protein P30 are implicated in the inhibition of virus internalization.
-
Application of a monoclonal antibody recognizing Protein P30 to detect African swine fever virus-infected cells in peripheral blood.
Journal of virological methods, 1995Co-Authors: Fernando Ramiro-ibáñez, J.m. Escribano, Covadonga AlonsoAbstract:Monoclonal antibody (MAb) 174F11.8 recognizes an epitope of the African swine fever (ASF) virus-induced Protein, P30, a Protein expressed on the plasma membrane of infected cells. This MAb has been used to analyze infected cell populations in peripheral blood of experimentally inoculated pigs with a virulent or attenuated ASF virus. Flow cytometric analysis of peripheral blood at different days postinfection using this MAb, showed expression of P30 mainly in the monocyte/macrophage cell lineage. Additionally, a small percentage of granulocytes also expressed P30 during infection. This methodology allowed the quantification of fluctuations in the pool of infected monocyte/macrophage cells in the inoculated pigs, maximum percentages ranging between 6 and 31%. Significant differences in the percentages of cell populations expressing P30 were not found between virulent or attenuated virus infection. However, a 2- to 4-day delay in the maximum percentage of cells expressing P30 was observed during infection with the attenuated virus when compared to virulent virus infection. Percentages of infected cells detected by the expression of P30 and viral titres obtained in peripheral blood showed positive correlation. Consequently, MAb 174F11.8 constitutes a marker to follow evolution of ASF virus infection, allowing quantification of percentages of infected cells in peripheral blood.
J.m. Escribano - One of the best experts on this subject based on the ideXlab platform.
-
Serodiagnosis of African swine fever using the recombinant Protein P30 expressed in insect larvae.
Journal of Virological Methods, 2000Co-Authors: Maria G. Barderas, Andrés Wigdorovitz, F Merelo, Francisco J. Beitia, Covadonga Alonso, M.v. Borca, J.m. EscribanoAbstract:African swine fever (ASF) has a substantial economic impact in many African developing countries and its eradication is based only on an efficient diagnosis program because of the absence of an available vaccine. Previous data suggested the convenience of using the highly antigenic virus Protein P30 as ELISA antigen for serological diagnosis of this disease. A simple and efficient method is described for producing the recombinant Protein P30 from ASF virus in Trichoplusia ni larvae (cabbage looper) in order to facilitate the large-scale production of this recombinant Protein in the absence of fermentation procedures. A baculovirus encoding the virus Protein P30 was used to infect insect larvae, showing that recombinant Protein production had a sharp optimal peak with a time of occurrence dependent on the initial virus dose inoculated to the larvae. Crude lysates of infected larvae were used without further purification as coating antigen in ELISA to analyse a limited number of sera from natural or experimentally ASF virus infected pigs. Remarkably, the recombinant Protein obtained from a single infected larva was sufficient for serological diagnosis of at least 3750 serum samples. Recombinant P30 obtained by this procedure was also used in a confirmatory immunoblotting, reacting with all positive sera tested previously by ELISA. In conclusion, production of the recombinant ASF virus Protein P30 in larvae should be applicable to large-scale production of diagnostic reagents for this disease in developing countries, eliminating the need for specialised facilities for tissue culture.
-
Neutralizing antibodies to different Proteins of African swine fever virus inhibit both virus attachment and internalization.
Journal of virology, 1996Co-Authors: Paulino Gómez-puertas, Covadonga Alonso, Fernando Ramiro-ibáñez, Fernando Rodriguez, J.m. Oviedo, F. Ruiz-gonzalvo, J.m. EscribanoAbstract:African swine fever virus induces in convalescent pigs antibodies that neutralized the virus before and after binding to susceptible cells, inhibiting both virus attachment and internalization. A further analysis of the neutralization mechanisms mediated by the different viral Proteins showed that antibodies to Proteins p72 and p54 are involved in the inhibition of a first step of the replication cycle related to virus attachment, while antibodies to Protein P30 are implicated in the inhibition of virus internalization.
-
Application of a monoclonal antibody recognizing Protein P30 to detect African swine fever virus-infected cells in peripheral blood.
Journal of virological methods, 1995Co-Authors: Fernando Ramiro-ibáñez, J.m. Escribano, Covadonga AlonsoAbstract:Monoclonal antibody (MAb) 174F11.8 recognizes an epitope of the African swine fever (ASF) virus-induced Protein, P30, a Protein expressed on the plasma membrane of infected cells. This MAb has been used to analyze infected cell populations in peripheral blood of experimentally inoculated pigs with a virulent or attenuated ASF virus. Flow cytometric analysis of peripheral blood at different days postinfection using this MAb, showed expression of P30 mainly in the monocyte/macrophage cell lineage. Additionally, a small percentage of granulocytes also expressed P30 during infection. This methodology allowed the quantification of fluctuations in the pool of infected monocyte/macrophage cells in the inoculated pigs, maximum percentages ranging between 6 and 31%. Significant differences in the percentages of cell populations expressing P30 were not found between virulent or attenuated virus infection. However, a 2- to 4-day delay in the maximum percentage of cells expressing P30 was observed during infection with the attenuated virus when compared to virulent virus infection. Percentages of infected cells detected by the expression of P30 and viral titres obtained in peripheral blood showed positive correlation. Consequently, MAb 174F11.8 constitutes a marker to follow evolution of ASF virus infection, allowing quantification of percentages of infected cells in peripheral blood.
B G Mclean - One of the best experts on this subject based on the ideXlab platform.
-
Tobacco mosaic virus movement Protein associates with the cytoskeleton in tobacco cells.
The Plant cell, 1995Co-Authors: B G Mclean, J Zupan, Patricia ZambryskiAbstract:Tobacco mosaic virus movement Protein P30 complexes with genomic viral RNA for transport through plasmodesmata, the plant intercellular connections. Although most research with P30 focuses on its targeting to and gating of plasmodesmata, the mechanisms of P30 intracellular movement to plasmodesmata have not been defined. To examine P30 intracellular localization, we used tobacco protoplasts, which lack plasmodesmata, for transfection with plasmids carrying P30 coding sequences under a constitutive promoter and for infection with tobacco mosaic virus particles. In both systems, P30 appears as filaments that colocalize primarily with microtubules. To a lesser extent, P30 filaments colocalize with actin filaments, and in vitro experiments suggested that P30 can bind directly to actin and tubulin. This association of P30 with cytoskeletal elements may play a critical role in intracellular transport of the P30-viral RNA complex through the cytoplasm to and possibly through plasmodesmata.
