The Experts below are selected from a list of 84 Experts worldwide ranked by ideXlab platform
Kirk Overmyer - One of the best experts on this subject based on the ideXlab platform.
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A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence data
IMA Fungus, 2021Co-Authors: Kai Wang, Timo Sipila, Kirk OvermyerAbstract:Protomyces is an understudied genus of yeast-like fungi currently defined as phytopathogens of only Umbelliferae and Compositae . Species relationships and boundaries remain controversial and molecular data are lacking. Of the 82 named Protomyces, we found few recent studies and six available cultures. We previously isolated Protomyces strains from wild Arabidopsis thaliana, a member of Brassicaceae , a family distant from accepted Protomyces hosts. We previously sequenced the genomes of all available Protomyces species, and P. arabidopsidicola sp. nov. strain C29, from Arabidopsis . Phylogenomics suggests this new species occupied a unique position in the genus. Genomic, morphological, and physiological characteristics distinguished P. arabidopsidicola sp. nov. from other Protomyces . Nuclear gene phylogenetic marker analysis suggests actin1 gene DNA sequences could be used with nuclear ribosomal DNA internal transcribed spacer sequences for rapid identification of Protomyces species. Previous studies demonstrated P. arabidopsidicola sp. nov. could persist on the Arabidopsis phyllosphere and Protomyces sequences were discovered on Arabidopsis at multiple sites in different countries. We conclude that the strain C29 represents a novel Protomyces species and propose the name of P. arabidopsidicola sp . nov. Consequently, we propose that Protomyces is not strictly associated only with the previously recognized host plants.
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a novel arabidopsis phyllosphere resident Protomyces sp and a re examination of genus Protomyces based on genome sequence data
bioRxiv, 2019Co-Authors: Kai Wang, Timo Sipila, Kirk OvermyerAbstract:ABSTRACT Protomyces is a genus of yeast-like fungi that is currently defined as plant pathogens of only the Umbelliferae (Apiaceae) and Compositae (Asteraceae) family plants. Many Protomyces species have been proposed; however, there is a lack of molecular data and available specimens for Protomyces spp., just over ten species are officially accepted and only six species are preserved and available in public culture collections for examination. Phylogenetic relationships and species boundaries within this genus remain largely controversial. Recently, we isolated Protomyces strains from wild Arabidopsis thaliana (Arabidopsis), a Brassicaceae family plant only distantly related to the accepted Protomyces hosts. We have previously sequenced the genomes of all the currently public available Protomyces species, together with a strain (SC29) we isolated from the Arabidopsis phyllosphere. Genome-wide phylogenetic analysis suggests that SC29 occupies a unique phylogenetic position in the genus Protomyces. The SC29 genome has low average nucleotide identity values in comparison with other species genomes. As physiological evidence, SC29 has morphological characteristics and carbon assimilation patterns that distinguish it from the other six Protomyces species. Analysis with several nuclear gene phylogenetic markers further confirms SC29 as a novel Protomyces species and suggests the act1 gene DNA sequences can be used together with ITS sequences for the rapid identification of Protomyces species. In our previous study, SC29 could persist on the Arabidopsis phyllosphere in both short term laboratory and overwinter outdoor garden experiments and Protomyces spp. (or OTUs) were found in the Arabidopsis phyllosphere at multiple sites in both Finland and Germany. We conclude that SC29 is a novel Protomyces species isolated from Arabidopsis and propose the name of Protomyces arabidopsidicola sp. nov. Additionally, the genus Protomyces may not be strictly associated with only Compositae or Umbelliferae family host plants, as evidenced by SC29 and Protomyces strains previously isolated from plants in other families. The merging of two Protomyces species found to have high genomic similarity (P. inouyei and P. lactucaedebilis) is also proposed.
Ghazala Parveen - One of the best experts on this subject based on the ideXlab platform.
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Supplementing biocontrol agents with botanicals improved growth and yield of coriander (Coriandrum sativum L.) infected with Protomyces macrosporus Unger
Elsevier, 2018Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:This investigation was carried out to examine the effect of some biocontrol agents (Trichoderma viride, T. harzianum, Azotobacter chroococcum, Pseudomonas putida) and botanicals (Melia azedarach, Eucalyptus globulus, Calotropis procera) both alone and in combination on growth, yield and stem gall disease of coriander (Coriandrum sativum L.) caused by Protomyces macrosporus Unger in a pot experiment. Plants inoculated with P. macrosporus caused significant reduction in growth and yield attributes as compared to untreated control. Stem gall intensity was recorded 59% in plants treated with pathogen alone. The results revealed that, application of biocontrol agents / botanicals both alone and in combination caused significant reduction in stem gall intensity (20.33–84.82%) as compared to control. Among the tested biocontrol agents, T. viride was found superior in enhancing the growth and yield as well as reduction in stem gall intensity (44.37%). Application of biocontrol agents in soil supplemented with botanicals was found more beneficial in enhancing plant growth and yield attributes as well as reducing stem gall intensity (52.54–84.82%). Among various combinations tested T. viride + Melia azedarach and T. viride + E. globulus caused greater increase in plant growth and yield characters both in pathogen inoculated as well as uninoculated plants. Keywords: Biocontrol agents, Botanicals, Protomyces macrosporus, Stem gall, Coriande
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Supplementing biocontrol agents with botanicals improved growth and yield of coriander (Coriandrum sativum L.) infected with Protomyces macrosporus Unger
Current Plant Biology, 2018Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:Abstract This investigation was carried out to examine the effect of some biocontrol agents ( Trichoderma viride , T. harzianum , Azotobacter chroococcum , Pseudomonas putida ) and botanicals ( Melia azedarach , Eucalyptus globulus , Calotropis procera ) both alone and in combination on growth, yield and stem gall disease of coriander ( Coriandrum sativum L.) caused by Protomyces macrosporus Unger in a pot experiment. Plants inoculated with P. macrosporus caused significant reduction in growth and yield attributes as compared to untreated control. Stem gall intensity was recorded 59% in plants treated with pathogen alone. The results revealed that, application of biocontrol agents / botanicals both alone and in combination caused significant reduction in stem gall intensity (20.33–84.82%) as compared to control. Among the tested biocontrol agents, T. viride was found superior in enhancing the growth and yield as well as reduction in stem gall intensity (44.37%). Application of biocontrol agents in soil supplemented with botanicals was found more beneficial in enhancing plant growth and yield attributes as well as reducing stem gall intensity (52.54–84.82%). Among various combinations tested T. viride + Melia azedarach and T. viride + E. globulus caused greater increase in plant growth and yield characters both in pathogen inoculated as well as uninoculated plants.
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Screening of Some Recently Developed Coriander Varieties against Stem Gall Disease Caused by Protomyces macrosporus
Journal of Plant Pathology & Microbiology, 2016Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:Twenty-seven varieties of coriander were evaluated for their resistance against stem gall disease caused by Protomyces macrosporus. Out of 27 varieties screened only four varieties viz., UD-125, UD-317, UD-749 and Rlr- 480 having less than 10% stem gall intensity were graded as resistant. The mean yield of healthy seeds was found between 1.33 to 1.97 g/plant and of diseased seeds was between 0.13 to 0.68 g/plant. Average seed yield loss (%) ranged between 6.04 to 27.87%. Minimum yield loss was recorded in UD-125 and maximum in American green.
Kai Wang - One of the best experts on this subject based on the ideXlab platform.
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A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence data
IMA Fungus, 2021Co-Authors: Kai Wang, Timo Sipila, Kirk OvermyerAbstract:Protomyces is an understudied genus of yeast-like fungi currently defined as phytopathogens of only Umbelliferae and Compositae . Species relationships and boundaries remain controversial and molecular data are lacking. Of the 82 named Protomyces, we found few recent studies and six available cultures. We previously isolated Protomyces strains from wild Arabidopsis thaliana, a member of Brassicaceae , a family distant from accepted Protomyces hosts. We previously sequenced the genomes of all available Protomyces species, and P. arabidopsidicola sp. nov. strain C29, from Arabidopsis . Phylogenomics suggests this new species occupied a unique position in the genus. Genomic, morphological, and physiological characteristics distinguished P. arabidopsidicola sp. nov. from other Protomyces . Nuclear gene phylogenetic marker analysis suggests actin1 gene DNA sequences could be used with nuclear ribosomal DNA internal transcribed spacer sequences for rapid identification of Protomyces species. Previous studies demonstrated P. arabidopsidicola sp. nov. could persist on the Arabidopsis phyllosphere and Protomyces sequences were discovered on Arabidopsis at multiple sites in different countries. We conclude that the strain C29 represents a novel Protomyces species and propose the name of P. arabidopsidicola sp . nov. Consequently, we propose that Protomyces is not strictly associated only with the previously recognized host plants.
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a novel arabidopsis phyllosphere resident Protomyces sp and a re examination of genus Protomyces based on genome sequence data
bioRxiv, 2019Co-Authors: Kai Wang, Timo Sipila, Kirk OvermyerAbstract:ABSTRACT Protomyces is a genus of yeast-like fungi that is currently defined as plant pathogens of only the Umbelliferae (Apiaceae) and Compositae (Asteraceae) family plants. Many Protomyces species have been proposed; however, there is a lack of molecular data and available specimens for Protomyces spp., just over ten species are officially accepted and only six species are preserved and available in public culture collections for examination. Phylogenetic relationships and species boundaries within this genus remain largely controversial. Recently, we isolated Protomyces strains from wild Arabidopsis thaliana (Arabidopsis), a Brassicaceae family plant only distantly related to the accepted Protomyces hosts. We have previously sequenced the genomes of all the currently public available Protomyces species, together with a strain (SC29) we isolated from the Arabidopsis phyllosphere. Genome-wide phylogenetic analysis suggests that SC29 occupies a unique phylogenetic position in the genus Protomyces. The SC29 genome has low average nucleotide identity values in comparison with other species genomes. As physiological evidence, SC29 has morphological characteristics and carbon assimilation patterns that distinguish it from the other six Protomyces species. Analysis with several nuclear gene phylogenetic markers further confirms SC29 as a novel Protomyces species and suggests the act1 gene DNA sequences can be used together with ITS sequences for the rapid identification of Protomyces species. In our previous study, SC29 could persist on the Arabidopsis phyllosphere in both short term laboratory and overwinter outdoor garden experiments and Protomyces spp. (or OTUs) were found in the Arabidopsis phyllosphere at multiple sites in both Finland and Germany. We conclude that SC29 is a novel Protomyces species isolated from Arabidopsis and propose the name of Protomyces arabidopsidicola sp. nov. Additionally, the genus Protomyces may not be strictly associated with only Compositae or Umbelliferae family host plants, as evidenced by SC29 and Protomyces strains previously isolated from plants in other families. The merging of two Protomyces species found to have high genomic similarity (P. inouyei and P. lactucaedebilis) is also proposed.
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a novel phyllosphere resident Protomyces species that interacts with the arabidopsis immune system
bioRxiv, 2019Co-Authors: Kai Wang, Timo Sipila, Sitaram Rajaraman, Omid Safronov, Pia Laine, Agate Auzane, Alfredo Mari, Petri Auvinen, Lars Paulin, Eric KemenAbstract:ABSTRACT We describe the genome contents of six Protomyces species that are pathogenic within the typical host range of the genus and a novel Protomyces strain (SC29) that was previously isolated from the phylloplane of wild Arabidopsis thaliana (Arabidopsis), an atypical host. Genome-wide phylogenetic analysis defined SC29 as a distinct Protomyces species. Analysis of gene family expansions, gene retention, and gene loss patterns among the Protomyces suggests that SC29 has recently undergone a host jump and change in lifestyle. Genomic changes in SC29 were consistent with a phylloplane lifestyle. SC29 did not cause disease on Arabidopsis, but could persist in its phylloplane, while the closely related P. inouyei does not. SC29 treated Arabidopsis exhibited enhanced immunity against Botrytis cinerea infection, associated with activation of MAPK3/6, camalexin, and SA-signalling pathways. We conclude that SC29 is a novel Protomyces species adapted to life in the Arabidopsis phylloplane.
Junta Sugiyama - One of the best experts on this subject based on the ideXlab platform.
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multiple origins of fungal group i introns located in the same position of nuclear ssu rrna gene
Journal of Molecular Evolution, 1998Co-Authors: Hiromi Nishida, Yoshito Tajiri, Junta SugiyamaAbstract:The archiascomycetous fungus Protomyces pachydermus has two group I introns within the nuclear small subunit (nSSU) rRNA gene. One of these introns has an internal open reading frame (ORF) that encodes a predicted protein of 228 amino acid residues. On the other hand, Protomyces macrosporus has two group I introns that insert at the same positions as P. pachydermus, which have no ORF. Each alignment was constructed with Protomyces group I introns located in the same position and other introns retrieved by the BLAST Search. Each phylogenetic tree based on the alignment shows that Protomyces introns are monophyletic but the relationships among fungal introns do not reflect on the fungal phylogeny. Therefore, it is suggested that two different horizontal transfers of group I introns occurred at the early stage of Protomyces species diversification.
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evolutionary relationships of members of the genera taphrina Protomyces schizosaccharomyces and related taxa within the archiascomycetes integrated analysis of genotypic and phenotypic characters
Mycoscience, 1997Co-Authors: Wellyzar Sjamsuridza, Hiromi Nishida, Yoshito Tajiri, Ta Bich Thuan, Hiroko Kawasaki, Aiko Hirata, Akira Yokota, Junta SugiyamaAbstract:To study the phylogeny and evolution of archiascomycetes, we determined the full sequence of the nuclear 18S rRNA gene from 14Taphrina species and 2Protomyces species, and the partial sequence ofSchizosaccharomyces japonicus var.japonicus. The sequences were phylogenetically analyzed by the neighbor-joining, maximum parsimony, and maximum-likelihood methods. We also looked at their principal phenotypic characters and genotypic character. Relationships within the Ascomycota are concordant with the previously published phylogenies inferred from 18S rDNA sequence divergence and divide the archi-, hemi-and euascomycetes into distinct major lineages. All the trees show that, within the archiascomycete lineage, 11 of the 14Taphrina species and the 2Protomyces species are monophyletic. A core groups ofTaphrina andProtomyces is always monophyletic. The evidence from molecular and phenotypic characters such as cell wall sugar composition, ubiquinone, cell wall ultrastructure, and mode of conidium ontogeny, strongly suggests that ‘T’. californica CBS 374.39, ‘T’. maculans CBS 427.69 and ‘T’. farlowii CBS 376.39 should be excluded from the archiascomycete lineage. ‘Taphrina’ farlowii CBS 376.39 groups withCandida albicans in the Saccharomycetales, whereas ‘T’. californica CBS 374.39 and ‘T’. maculans CBS 427.69 have a basidiomycete affinity and group with Tremellalean members in the hymenomycete lineage.Schizosaccharomyces is monophyletic. The strictly anamorphic yeastSaitoella complicata groups with the apothecial ascomyceteNeolecta vitellina rather than theTaphrina/Protomyces branch.
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the higher fungus Protomyces inouyei has two group i introns in the 18s rrna gene
Journal of Molecular Evolution, 1993Co-Authors: Hiromi Nishida, Paul A Blanz, Junta SugiyamaAbstract:The nucleotide sequence of the small-subunit rRNA (18S rRNA) coding gene in the higher fungus Protomyces inouyei contains two group I introns. This is the first report of two group I introns in the 18S rRNA coding region. Based on the comparison of the two introns of Protomyces inouyei with those of the green alga Ankistrodesmus stipitatus, and the other two higher fungi Pneumocystis carinii and Ustilago maydis, the Protomyces introns are group I introns containing the highly conserved sequence elements P, Q, R, and S. Intron A of Protomyces inouyei is located in the same position as in Pneumocystis carinii while intron B shares the location with that in Ustilago maydis. The phylogenetic relationships strongly support horizontal transfer of these group I introns.
Manzoor R. Khan - One of the best experts on this subject based on the ideXlab platform.
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Supplementing biocontrol agents with botanicals improved growth and yield of coriander (Coriandrum sativum L.) infected with Protomyces macrosporus Unger
Elsevier, 2018Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:This investigation was carried out to examine the effect of some biocontrol agents (Trichoderma viride, T. harzianum, Azotobacter chroococcum, Pseudomonas putida) and botanicals (Melia azedarach, Eucalyptus globulus, Calotropis procera) both alone and in combination on growth, yield and stem gall disease of coriander (Coriandrum sativum L.) caused by Protomyces macrosporus Unger in a pot experiment. Plants inoculated with P. macrosporus caused significant reduction in growth and yield attributes as compared to untreated control. Stem gall intensity was recorded 59% in plants treated with pathogen alone. The results revealed that, application of biocontrol agents / botanicals both alone and in combination caused significant reduction in stem gall intensity (20.33–84.82%) as compared to control. Among the tested biocontrol agents, T. viride was found superior in enhancing the growth and yield as well as reduction in stem gall intensity (44.37%). Application of biocontrol agents in soil supplemented with botanicals was found more beneficial in enhancing plant growth and yield attributes as well as reducing stem gall intensity (52.54–84.82%). Among various combinations tested T. viride + Melia azedarach and T. viride + E. globulus caused greater increase in plant growth and yield characters both in pathogen inoculated as well as uninoculated plants. Keywords: Biocontrol agents, Botanicals, Protomyces macrosporus, Stem gall, Coriande
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Supplementing biocontrol agents with botanicals improved growth and yield of coriander (Coriandrum sativum L.) infected with Protomyces macrosporus Unger
Current Plant Biology, 2018Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:Abstract This investigation was carried out to examine the effect of some biocontrol agents ( Trichoderma viride , T. harzianum , Azotobacter chroococcum , Pseudomonas putida ) and botanicals ( Melia azedarach , Eucalyptus globulus , Calotropis procera ) both alone and in combination on growth, yield and stem gall disease of coriander ( Coriandrum sativum L.) caused by Protomyces macrosporus Unger in a pot experiment. Plants inoculated with P. macrosporus caused significant reduction in growth and yield attributes as compared to untreated control. Stem gall intensity was recorded 59% in plants treated with pathogen alone. The results revealed that, application of biocontrol agents / botanicals both alone and in combination caused significant reduction in stem gall intensity (20.33–84.82%) as compared to control. Among the tested biocontrol agents, T. viride was found superior in enhancing the growth and yield as well as reduction in stem gall intensity (44.37%). Application of biocontrol agents in soil supplemented with botanicals was found more beneficial in enhancing plant growth and yield attributes as well as reducing stem gall intensity (52.54–84.82%). Among various combinations tested T. viride + Melia azedarach and T. viride + E. globulus caused greater increase in plant growth and yield characters both in pathogen inoculated as well as uninoculated plants.
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Screening of Some Recently Developed Coriander Varieties against Stem Gall Disease Caused by Protomyces macrosporus
Journal of Plant Pathology & Microbiology, 2016Co-Authors: Manzoor R. Khan, Ghazala ParveenAbstract:Twenty-seven varieties of coriander were evaluated for their resistance against stem gall disease caused by Protomyces macrosporus. Out of 27 varieties screened only four varieties viz., UD-125, UD-317, UD-749 and Rlr- 480 having less than 10% stem gall intensity were graded as resistant. The mean yield of healthy seeds was found between 1.33 to 1.97 g/plant and of diseased seeds was between 0.13 to 0.68 g/plant. Average seed yield loss (%) ranged between 6.04 to 27.87%. Minimum yield loss was recorded in UD-125 and maximum in American green.
