The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
Michiaki Masuda - One of the best experts on this subject based on the ideXlab platform.
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molecular characterization of prototheca strains isolated in china revealed the first cases of Protothecosis associated with prototheca zopfii genotype 1
Medical Mycology, 2018Co-Authors: Noriyuki Hirose, Qiangqiang Zhang, Kazuko Nishimura, Yuichi Kato, Ruoyu Li, Michiaki MasudaAbstract:: In this study, six strains of Prototheca isolated in China from human patients diagnosed as Protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human Protothecosis associated with P. zopfii genotype 1. The remaining three strains were shown to be P. zopfii genotype 2. Interestingly, one strain isolated from the cerebrospinal fluid of the human patient appeared to have both of the genotype 1- and 2-specific alleles in the small subunit (SSU) rDNA although it was classified by MALDI-TOF MS as genotype 2. For genotyping of certain strains of P. zopfii, it may be necessary to comprehensively evaluate the diversity in the SSU rDNA sequences and the MALDI-TOF MS results.
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case report of cutaneous Protothecosis caused by prototheca wickerhamii designated as genotype 2 and current status of human Protothecosis in japan
Journal of Dermatology, 2018Co-Authors: Maki Inoue, Azusa Miyashita, Hiromitsu Noguchi, Noriyuki Hirose, Kazuko Nishimura, Michiaki MasudaAbstract:: An 85-year-old Japanese woman presented with infiltrative erythema and ulceration on the extensor surface of her right forearm. Direct microscopic examination demonstrated spherical and morula-like sporangia, while histopathology revealed numerous microorganisms with a mulberry-like appearance in the dermis. Staining of the microorganisms also showed mulberry-like sporangia that resembled the spokes of a wheel. The isolated yeast-like microorganism had been identified as Prototheca wickerhamii genotype 2 in another independent study on the basis of its morphological, biochemical and genetic analysis. This case of Protothecosis was recorded in Kyushu, Japan, and oral treatment with itraconazole 200 mg/day for 2 months was effective. Herein, we also summarize and analyze 39 cases of human Protothecosis reported in Japan since the first record in 1983.
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disseminated Protothecosis manifesting with multiple rapidly progressing skin ulcers successful treatment with amphotericin b
European Journal of Dermatology, 2015Co-Authors: Kazuko Nishimura, Maho Murata, Konosuke Nagae, Misa Nakanonakamura, Ruriko Nishida, Kenjiro Takei, Masakazu Takahara, Hiroshi Uchi, Michiaki MasudaAbstract:Protothecosis is a rare infection caused by Prototheca species, which are achlorophyllous algae found ubiquitously in nature [1]. Herein, we report a case of disseminated P. wickerhamii infection in a patient with systemic lupus erythematosus (SLE). The disease presented as multiple, rapidly-progressing skin ulcers and was successfully treated with systemic antifungal agents.A 65-year-old Japanese woman was referred to our hospital with a two-week history of extensive ulcerative lesions on the right [...]
Maho Murata - One of the best experts on this subject based on the ideXlab platform.
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disseminated Protothecosis manifesting with multiple rapidly progressing skin ulcers successful treatment with amphotericin b
European Journal of Dermatology, 2015Co-Authors: Kazuko Nishimura, Maho Murata, Konosuke Nagae, Misa Nakanonakamura, Ruriko Nishida, Kenjiro Takei, Masakazu Takahara, Hiroshi Uchi, Michiaki MasudaAbstract:Protothecosis is a rare infection caused by Prototheca species, which are achlorophyllous algae found ubiquitously in nature [1]. Herein, we report a case of disseminated P. wickerhamii infection in a patient with systemic lupus erythematosus (SLE). The disease presented as multiple, rapidly-progressing skin ulcers and was successfully treated with systemic antifungal agents.A 65-year-old Japanese woman was referred to our hospital with a two-week history of extensive ulcerative lesions on the right [...]
S Wu - One of the best experts on this subject based on the ideXlab platform.
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Protothecosis successfully treated with amikacin combined with tetracyclines
Mycoses, 2004Co-Authors: Jingjun Zhao, Guixia Lv, Yongnian Shen, S WuAbstract:Summary We report a case of Protothecosis in an 18-year-old female student caused by Prototheca zopfii successfully treated with amikacin combined with tetracyclines. Zusammenfassung Es wird uber eine Protothecose, verursacht durch Prototheca zopfii, bei einer 18-jahrigen Studentin berichtet, die erfolgreich mit Amikacin in Kombination mit Tetracyclinen behandelt wurde.
Shelly L Vaden - One of the best experts on this subject based on the ideXlab platform.
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urinary tract manifestations of Protothecosis in dogs
Journal of Veterinary Internal Medicine, 2005Co-Authors: Barrak M Pressler, Jody L Gookin, Jane E Sykes, Alice M Wolf, Shelly L VadenAbstract:: Records of 13 dogs with systemic infection with Prototheca sp. from 3 veterinary teaching hospitals were reviewed. Acute renal failure secondary to disseminated infection with Prototheca zopfii was diagnosed in 2 dogs. In 1 dog, acute renal failure developed during administration of immunosuppressive drugs for treatment of anterior uveitis. During diagnostic evaluation of this dog, Prototheca sp. organisms were noted in urine sediment and renal biopsy specimens. In the 2nd dog, acute renal failure was diagnosed after treatment for bacterial cystitis. After diagnosis of Protothecosis, organisms were successfully isolated by aerobic urine culture. Both dogs with acute renal failure did not respond to conventional medical therapy. In total, Prototheca sp. was noted in urine sediment in 4 of 8 dogs and successfully cultured from urine in 5 of 7 dogs. Four of 5 dogs had organisms noted in the kidneys on histopathologic examination. In all dogs, the species identified was P zopfii. Sensitivity testing of 3 isolates revealed wide differences in in vitro drug resistance. Examination and culture of urine is recommended as a practical method for diagnosis of systemic infection with Prototheca sp.
D W W Newling - One of the best experts on this subject based on the ideXlab platform.
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Protothecosis of the urinary tract
The Journal of Urology, 2002Co-Authors: B P J Van Bezooijen, D W W NewlingAbstract:A 62-year-old woman was seen with recurrent urinary tract infections, frequency and hematuria. Cystoscopy showed signs of inflammation, including edematous, hyperemic urothelium and turbid urine with much debris. An excretory urogram, ultrasound and blood analysis showed no abnormalities. Urine cultures yielded Prototheca zopfii sensitive to itraconazole, flucytosine and amphotericin B. The patient was treated with itraconazole orally for 3 months and initially the symptoms disappeared but frequency and dysuria recurred. Urine cultures again yielded P. zopfii. The patient was subsequently treated with bladder instillation of 50 mg./l. amphotericin B 6 times in 3 weeks. As symptoms persisted she was admitted to the hospital and treated with continuous flushing of the bladder with 50 mg./l. amphotericin B, and 200 mg. itraconazole twice a day and 250 mg. terbinafine once a day orally for 7 days. After cessation of this therapy symptoms subsided and repeat cultures showed no sign of infection. DISCUSSION
