The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Caterina Mammina - One of the best experts on this subject based on the ideXlab platform.
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Salmonella bongori provides insights into the evolution of the Salmonellae
PLOS Pathogens, 2011Co-Authors: Maria Fookes, Caterina Mammina, Gunnar N Schroeder, Gemma C Langridge, Carlos J Blondel, Thomas R Connor, Helena M B Sethsmith, Georgios S Vernikos, Keith S Robinson, Mandy SandersAbstract:The genus Salmonella contains two species, S. bongori and S. enterica. Compared to the well-studied S. enterica there is a marked lack of information regarding the genetic makeup and diversity of S. bongori. S. bongori has been found predominantly associated with cold-blooded animals, but it can infect humans. To define the phylogeny of this species, and compare it to S. enterica, we have sequenced 28 isolates representing most of the known diversity of S. bongori. This cross-species analysis allowed us to confidently differentiate ancestral functions from those acquired following speciation, which include both metabolic and virulence-associated capacities. We show that, although S. bongori inherited a basic set of Salmonella common virulence functions, it has subsequently elaborated on this in a different direction to S. enterica. It is an established feature of S. enterica evolution that the acquisition of the type III secretion systems (T3SS-1 and T3SS-2) has been followed by the sequential acquisition of genes encoding secreted targets, termed effectors proteins. We show that this is also true of S. bongori, which has acquired an array of novel effector proteins (sboA-L). All but two of these effectors have no significant S. enterica homologues and instead are highly similar to those found in enteropathogenic Escherichia coli (EPEC). Remarkably, SboH is found to be a chimeric effector protein, encoded by a fusion of the T3SS-1 effector gene sopA and a gene highly similar to the EPEC effector nleH from enteropathogenic E. coli. We demonstrate that representatives of these new effectors are translocated and that SboH, similarly to NleH, blocks intrinsic apoptotic pathways while being targeted to the mitochondria by the SopA part of the fusion. This work suggests that S. bongori has inherited the ancestral Salmonella virulence gene set, but has adapted by incorporating virulence determinants that resemble those employed by EPEC.
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Salmonella bongori 48 z35 in migratory birds italy
Emerging Infectious Diseases, 2009Co-Authors: Maria Foti, Antonio Daidone, Aurora Aleo, Alessia Pizzimenti, C Giacopello, Caterina MamminaAbstract:Suggested citation for this article: Foti M, Daidone A, Aleo A, Pizzimenti A, Giacopello C, Mammina C. Salmonella bongori 48:z35:– in migratory birds, Italy. Emerg Infect Dis [serial on the Internet]. 2009 Mar [date cited]. Available from http://www.cdc.gov/EID/content/15/3/502.htm
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persistent endemicity of Salmonella bongori 48 z 35 in southern italy molecular characterization of human animal and environmental isolates
Journal of Clinical Microbiology, 2002Co-Authors: Giovanni M Giammanco, Caterina Mammina, S Pignato, Francine Grimont, Patrick A D Grimont, Antonino Nastasi, G GiammancoAbstract:From 1984 to 1999, we collected 31 isolates of the rare serovar Salmonella bongori 48:z35:− in southern Italy. Twenty-four of the isolates were from cases of acute enteritis in humans. Pulsed-field gel electrophoresis analysis showed that all but one of our isolates were at least 80% similar. Our findings suggest that genetically related S. bongori 48:z35:− strains are endemically circulating in southern Italy.
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epidemia di Salmonella bongori 48 z35 enterite in sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z35:- e stata isolata per la prima volta, nel 1966, in Chad da una lucertola ed e stata classificata come ceppo biochimicamente atipico del sottogenere I di Kauffmann (1). In seguito, sono stati identificati altri ceppi, con formule antigeniche diverse ma con proprieta biochimiche simili, che sono stati assegnati al 'gruppo bongoro' del genere Salmonella (2). Piu recentemente, e stato appurato, sulla base di caratteristiche genetiche e biochimiche, che S. bongori costituisce una specie diversa di Salmonella (3).
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brote de gastroenteritis por Salmonella bongori 48 z35 en sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:La Salmonella bongori 48:z35:- se aislo por primera vez en 1966 en una lagartija de Chad y se clasifico como una cepa bioquimicamente atipica del subgenero I de Kauffmann (1). Posteriormente, se identificaron otras cepas con distintas formulas antigenicas, pero con propiedades bioquimicas similares, y se asignaron al ‘grupo bongor’ del genero Salmonella (2). Ultimamente, por sus caracteristicas geneticas y bioquimicas, se ha definido a la S. bongori como una especie de Salmonella distinta (3).
L Salsa - One of the best experts on this subject based on the ideXlab platform.
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epidemia di Salmonella bongori 48 z35 enterite in sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z35:- e stata isolata per la prima volta, nel 1966, in Chad da una lucertola ed e stata classificata come ceppo biochimicamente atipico del sottogenere I di Kauffmann (1). In seguito, sono stati identificati altri ceppi, con formule antigeniche diverse ma con proprieta biochimiche simili, che sono stati assegnati al 'gruppo bongoro' del genere Salmonella (2). Piu recentemente, e stato appurato, sulla base di caratteristiche genetiche e biochimiche, che S. bongori costituisce una specie diversa di Salmonella (3).
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brote de gastroenteritis por Salmonella bongori 48 z35 en sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:La Salmonella bongori 48:z35:- se aislo por primera vez en 1966 en una lagartija de Chad y se clasifico como una cepa bioquimicamente atipica del subgenero I de Kauffmann (1). Posteriormente, se identificaron otras cepas con distintas formulas antigenicas, pero con propiedades bioquimicas similares, y se asignaron al ‘grupo bongor’ del genero Salmonella (2). Ultimamente, por sus caracteristicas geneticas y bioquimicas, se ha definido a la S. bongori como una especie de Salmonella distinta (3).
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outbreak of Salmonella enteritis bongori 48 z35 in sicily
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z 35 :- was first isolated from a lizard in Chad in 1966 and was classified as a biochemically atypical strain of the subgenus I of Kauffmann. Successively, some additional strains with different antigenic formulas but similar bioche
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epidemie de Salmonella enteritis bongori 48 z35 en sicile
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z35:- a ete isolee pour la premiere fois en 1966 chez un lezard au Tchad et a alors ete classee comme une souche atypique sur le plan biochimique appartenant au sous-genre I de Kauffmann (1). Par la suite, d’autres souches partageant les memes proprietes biochimiques, mais se differenciant par leurs formules antigeniques ont ete identifiees et attribuees au "groupe bongor" du genre Salmonella (2). Plus recemment, sur la base de ses caracteristiques genetiques et biochimiques, S. bongori a ete definie comme une espece de salmonelle distincte (3).
A Nastasi - One of the best experts on this subject based on the ideXlab platform.
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epidemia di Salmonella bongori 48 z35 enterite in sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z35:- e stata isolata per la prima volta, nel 1966, in Chad da una lucertola ed e stata classificata come ceppo biochimicamente atipico del sottogenere I di Kauffmann (1). In seguito, sono stati identificati altri ceppi, con formule antigeniche diverse ma con proprieta biochimiche simili, che sono stati assegnati al 'gruppo bongoro' del genere Salmonella (2). Piu recentemente, e stato appurato, sulla base di caratteristiche genetiche e biochimiche, che S. bongori costituisce una specie diversa di Salmonella (3).
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brote de gastroenteritis por Salmonella bongori 48 z35 en sicilia
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:La Salmonella bongori 48:z35:- se aislo por primera vez en 1966 en una lagartija de Chad y se clasifico como una cepa bioquimicamente atipica del subgenero I de Kauffmann (1). Posteriormente, se identificaron otras cepas con distintas formulas antigenicas, pero con propiedades bioquimicas similares, y se asignaron al ‘grupo bongor’ del genero Salmonella (2). Ultimamente, por sus caracteristicas geneticas y bioquimicas, se ha definido a la S. bongori como una especie de Salmonella distinta (3).
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outbreak of Salmonella enteritis bongori 48 z35 in sicily
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z 35 :- was first isolated from a lizard in Chad in 1966 and was classified as a biochemically atypical strain of the subgenus I of Kauffmann. Successively, some additional strains with different antigenic formulas but similar bioche
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epidemie de Salmonella enteritis bongori 48 z35 en sicile
Eurosurveillance, 1999Co-Authors: A Nastasi, Caterina Mammina, L SalsaAbstract:Salmonella bongori 48:z35:- a ete isolee pour la premiere fois en 1966 chez un lezard au Tchad et a alors ete classee comme une souche atypique sur le plan biochimique appartenant au sous-genre I de Kauffmann (1). Par la suite, d’autres souches partageant les memes proprietes biochimiques, mais se differenciant par leurs formules antigeniques ont ete identifiees et attribuees au "groupe bongor" du genre Salmonella (2). Plus recemment, sur la base de ses caracteristiques genetiques et biochimiques, S. bongori a ete definie comme une espece de salmonelle distincte (3).
Jorg Vogel - One of the best experts on this subject based on the ideXlab platform.
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a small non coding rna of the invasion gene island spi 1 represses outer membrane protein synthesis from the Salmonella core genome
Molecular Microbiology, 2007Co-Authors: Verena Pfeiffer, Alexandra Sittka, Raju Tomer, Karsten Tedin, Volker Brinkmann, Jorg VogelAbstract:Summary The Salmonella pathogenicity island (SPI-1) encodes ~35 proteins involved in assembly of a type III secre- tion system (T3SS) which endows Salmonella with the ability to invade eukaryotic cells. We have discovered a novel SPI-1 gene, invR, which expresses an abun- dant small non-coding RNA (sRNA). The invR gene, which we identified in a global search for new Salmo- nella sRNA genes, is activated by the major SPI-1 transcription factor, HilD, under conditions that favour host cell invasion. The RNA chaperone, Hfq, is essential for the in vivo stability of the ~80 nt InvR RNA. Hfq binds InvR with high affinity in vitro, and InvR co-immunoprecipitates with FLAG epitope- tagged Hfq in Salmonella extracts. Surprisingly, deletion/overexpression of invR revealed no pheno- type in SPI-1 regulation. In contrast, we find that InvR represses the synthesis of the abundant OmpD porin encoded by the Salmonella core genome. As invR is conserved in the early branching Salmonella bongori, we speculate that porin repression by InvR may have aided successful establishment of the SPI-1 T3SS after horizontal acquisition in the Salmonella lineage. This study identifies the first regulatory RNA of an enterobacterial pathogenicity island, and new roles for Hfq and HilD in SPI-1 gene expression.
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a small non coding rna of the invasion gene island spi 1 represses outer membrane protein synthesis from the Salmonella core genome
Molecular Microbiology, 2007Co-Authors: Verena Pfeiffer, Alexandra Sittka, Raju Tomer, Karsten Tedin, Volker Brinkmann, Jorg VogelAbstract:The Salmonella pathogenicity island (SPI-1) encodes approximately 35 proteins involved in assembly of a type III secretion system (T3SS) which endows Salmonella with the ability to invade eukaryotic cells. We have discovered a novel SPI-1 gene, invR, which expresses an abundant small non-coding RNA (sRNA). The invR gene, which we identified in a global search for new Salmonella sRNA genes, is activated by the major SPI-1 transcription factor, HilD, under conditions that favour host cell invasion. The RNA chaperone, Hfq, is essential for the in vivo stability of the approximately 80 nt InvR RNA. Hfq binds InvR with high affinity in vitro, and InvR co-immunoprecipitates with FLAG epitope-tagged Hfq in Salmonella extracts. Surprisingly, deletion/overexpression of invR revealed no phenotype in SPI-1 regulation. In contrast, we find that InvR represses the synthesis of the abundant OmpD porin encoded by the Salmonella core genome. As invR is conserved in the early branching Salmonella bongori, we speculate that porin repression by InvR may have aided successful establishment of the SPI-1 T3SS after horizontal acquisition in the Salmonella lineage. This study identifies the first regulatory RNA of an enterobacterial pathogenicity island, and new roles for Hfq and HilD in SPI-1 gene expression.
Perumal Pachiappan - One of the best experts on this subject based on the ideXlab platform.
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evaluation of Salmonella bongori derived biosurfactants and its extracellular protein separation by sds page using petridishes a simply modified approach
International Journal of Biological Macromolecules, 2019Co-Authors: Balasubramani Govindasamy, Lorenzo Pecoraro, Balasubramanian Velramar, Silambarasan Tamilselvan, Ayyasamy Pudukadu Munusamy, Ragavendran Chinnasamy, Perumal PachiappanAbstract:Abstract Presently, through the preliminary screening assays, the Salmonella bongori BH11 was found to be an effective biosurfactants (BSFs) producer. The secreted BSFs were extracted using methanol: chloroform and characterized through FTIR, TLC, HPLC and GCMS analyses. Further, the extracellular protein was extracted (TCA/acetone method), estimated (Lowry's method) and separated (standard and modified SDS-PAGE). Through the obtained characteristic FTIR peaks (1107.09 cm−1), its content was presumed to be glycolipids and as rhamnose/rhamnolipids through the TLC-Rf value. GCMS revealed 6 compounds, in which Toluene (32%) and 5-(2-Thienyl) pentanoic acid (23%) are the major ones. The crude BSFs exhibited preponderant antibacterial effects on Staphylococcus aureus and Serratia marcescens. Also, it inhibited the biofilm formation of S. aureus, Pseudomonas aeruginosa, P. fluorescens and S. marcescens. Considerably, 76% mortality of IV instar larvae of Culex quinquefasciatus was recorded from BSFs, when compared to SDS. The presently followed protein separation technique using two petridishes might attract the attention of the researchers, as it would emerge as a standard procedure in future. This is the first report on the screening of BSFs from Salmonella bongori that showed antagonistic property, larvicidal potentials and the presently followed modified SDS-PAGE protein separation technique is a simple, reliable and cost effective one.
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comparative extraction of Salmonella bongori derived metabolites and their toxicity on bacterial pathogens mosquito larvae zebrafish embryo and brine shrimp a modified approach
Ecotoxicology and Environmental Safety, 2019Co-Authors: Balasubramani Govindasamy, Deepak Paramasivam, Aiswarya Dilipkumar, Kamaraj Chinnaperumal, Arul Dhayalan, Jothimani Kannupaiyan, Santhanam Perumal, Perumal PachiappanAbstract:Abstract The present study pertains to two different (standard and adapted) extraction-procedures to extract bacterial extracellular metabolites from the cell-free supernatant (CFS) of S. bongori. Metabolites were extracted with the different polarity solvents using lyophilized-CFS mediated procedure, which revealed more number of compounds than standard procedure. The crude-extracts (CEs) were characterized using FTIR, HPLC and GC-MS analyses. The commonly presented compounds in standard (ME, EA & HE) and lyophilization-mediated extracts (LME, LEA & LHE) were identified through Heat-map analysis. Antibacterial assay: all CEs showed considerable activity on tested MTCC-strains, in which, LME and LEA were found preponderant. Larvicidal bioassay: LME resulted maximum mortality than other CEs on Culex-larvae. Zebrafish embryo-toxicity assay: except HE, all CEs exhibited toxicity at 100 ppm after 96 hpf. Brine shrimp-toxicity assay: ME, LME, EA and LEA have shown significant mortality after 24 h. With these observations, the adapted-extraction-procedure could form significance in the drug development process.
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Multipurpose efficacy of the lyophilized cell-free supernatant of Salmonella bongori isolated from the freshwater fish, Devario aequipinnatus: toxicity against microbial pathogens and mosquito vectors
Environmental Science and Pollution Research, 2018Co-Authors: Balasubramani Govindasamy, Deepak Paramasivam, Aiswarya Dilipkumar, Karthik Raja Ramalingam, Kamaraj Chinnaperumal, Perumal PachiappanAbstract:Presently, the discovery of effective drugs and pesticides from eco-friendly biological sources is an important challenge in the field of life sciences. The present research was aimed for standardizing an innovative approach in the evaluation of the biological potentiality of the metabolites of fish-associated bacteria. We have identified 17 skin-associated bacteria from the freshwater fish, giant danio, Devario aquipinnatus. They were screened through biofilm forming and extracellular enzyme producing ability. The results of preliminary antibacterial evaluation of the bacterial supernatants underlined the importance of three potential strains (BH8, BH10 and BH11) for further applied research. Hence, such strains were subsequently subjected to a novel extraction procedure to overcome the difficulties found in polar solvents mixed with the supernatant. The lyophilized cell-free supernatant (LCFS) of 3 isolates were individually extracted by using methanol. During the testing of LCFS’s methanolic extract (LCFS-ME) of 3 isolates, only the extract of BH11-strain exhibited potent inhibitory activity against the pathogenic bacteria and fungi. Furthermore, the larvicidal and mosquitocidal assays on the filariasis vector, Culex quinquefasciatus also showed its potent toxicity on both the adults and developmental instars of mosquito . Through molecular and phylogenetic analyses, the BH11 strain was identified as Salmonella bongori (KR350635). The present finding emphasized that the S. bongori could be an important novel source of effective antimicrobials and mosquitocidal agents.
