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Jongki Hong - One of the best experts on this subject based on the ideXlab platform.
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gc ms combined with chemometrics methods for quality control of Schizonepeta tenuifolia briq determination of essential oils
Microchemical Journal, 2011Co-Authors: Mihee Chun, Eunkyung Kim, Kiyoung Moon, Jee H Jung, Jongki HongAbstract:Abstract A simple and rapid gas chromatography/mass spectrometry (GC/MS) analysis method was developed for the determination of essential oils in the crude extract of Schizonepeta tenuifolia Briq (Sch.t.Briq). Five major volatiles (menthone, pulegone, 2-hydroxy-2-isopropenyl-5-methylcylohexanone, cis-pulegone oxide, and schizonal) were extracted and isolated from Sch.t.Briq as marker compounds for use in the quality control of herbal medicines. Various extraction techniques, such as solvent immersion, mechanical shaking, and sonication, were evaluated, and the greatest efficiency was observed with sonication extraction using petroleum ether. The dynamic range of the GC/MS method depended on the specific analyte; acceptable quantification was obtained between 10 and 1000 μg/mL for menthone and pulegone, and between 2.5 and 75.0 μg/mL for 2-hydroxy-2-isopropenyl-5-methylcylohexanone, cis-pulegone oxide, and schizonal. The method was deemed satisfactory by inter- and intra-day validation and exhibited both high accuracy and precision, with a relative standard deviation
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quality control of Schizonepeta tenuifolia briq by solid phase microextraction gas chromatography mass spectrometry and principal component analysis
Microchemical Journal, 2010Co-Authors: Mihee Chun, Kangro Lee, Eunkyung Kim, Jee H Jung, Jongki HongAbstract:article i nfo The chemical composition of Schizonepeta tenuifolia Briq. (Sch.t.Briq.) is mainly composed of several volatile substances that affect multiple pharmacological targets and provide clinical efficacy. In this work, a headspace/solid-phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) method was developed to evaluate the profiles of volatile compounds in Sch.t.Briq. The optimization of SPME conditions was carried out using four kinds of fiber, extraction time and temperature, desorption temperature and time, and sample amount. The GC/MS analysis allowed the tentative identification of 21 compounds, with similarities higher than 85%, in accordance with the NIST/Wiley mass spectral library. Major components such as (+)-menthone (14.32%), (−)-pulegone (47.73%), 2-hydroxy-2-isopropenyl-5- methylcyclohexane (5.97%), cis-pulegone oxide (4.12%), and schizonal (5.36%) were identified by comparison of retention time and mass spectral data of standards isolated from Sch.t.Briq. The contents of these compounds were about above 78% against total amounts of volatile compounds extracted from Sch.t. Briq. Based on optimized SPME method, 19 different Sch.t.Briq. samples collected from markets in Korea and China were analyzed to obtain the profiling data of volatile compounds. In addition, principal component analysis (PCA) was performed on the profiling data in order to classify the samples collected from the different regions. PCA could possibly visualize the grouping tendencies of the studied varieties of herbal samples, as well as the identification of the volatiles responsible for discriminating the groups.
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phytochemical constituents of Schizonepeta tenuifolia briquet
Natural product sciences, 2008Co-Authors: Ilkyun Lee, Minah Kim, Seung Young Lee, Jongki Hong, Jeihyun Lee, Kangro LeeAbstract:Column chromatographic separation of the MeOH extract from the aerial parts of Schizonepeta tenuifolia Briquet led to the isolation of twelve terpenes (1 - 11 and 17), four phenolics (13 - 16) and a hexenyl glucoside (12). Their structures were determined by spectroscopic means to be (-)-pulegone (1), piperitenone (2), p-cymene-3,8-diol (3), schizonepetoside A (4), schizonepetoside C (5), (+)-spatulenol (6), ursolic acid (7), ,,,-trihydroxyolean-12en-28oic acid (8), ,-epidioxyergosta-6,22-diol--ol (9), stigmast-4-en-3-one (10), (11), (Z)-3-hexenyl-1-O--D-glucopyranoside (12), rosmarinic acid (13), apigenin-7-O--D-glucopyranoside (14), luteolin-7-O--D-glucuronopyranoside (15), hesperidin (16) and trans-phytol (17). Compounds 2, 3, 8, 9 and 12 were for the first time isolated from S. tenuifolia Briq.
Xi Bai - One of the best experts on this subject based on the ideXlab platform.
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spectrum effect relationship between gc qtof ms fingerprint and antioxidant anti inflammatory activities of Schizonepeta tenuifolia essential oil
Biomedical Chromatography, 2021Co-Authors: Xi Bai, Haji Akber Aisa, Liu Liu, Junping Zhang, Li Chen, Maitinuer MaiwulanjiangAbstract:Schizonepeta tenuifolia (Benth.) Briq (S. tenuifolia), a traditional Chinese medicine, is an annual herbaceous plant that is widely distributed in China, Japan, and Korea. The essential oil (EO) of S. tenuifolia has antioxidant and anti-inflammatory effects. However, the components contributing to its antioxidant and anti-inflammatory activities remain unclear. This study was aimed at investigating the spectrum-effect relationship between gas chromatography-mass spectrometry (GC-MS) fingerprint and the antioxidant and anti-inflammatory effects of S. tenuifolia EO. Herein, the fingerprints of EO from ten batches of S. tenuifolia from various sources were established using GC-MS, and the antioxidant and anti-inflammatory bioactivities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) inhibitory assays, respectively. Finally, 13 common peaks were identified from ten batches of S. tenuifolia, by searching against the standard mass spectra in NIST 14 and comparing the literature retention index. The different sources of S. tenuifolia EO exhibit mild antioxidant activities and significant anti-inflammatory effects. Specifically, menthone (peak 3), isomenthone (peak 4), pulegone (peak 7), piperitone (peak 8), and β-caryophyllene (peak 11) might be the dominant constituents responsible for the antioxidant and anti-inflammatory activities of S. tenuifolia EO. This method may provide a time-saving, convenient way to screen the potential effective components of S. tenuifolia EO.
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detection of antibacterial and antioxidant compounds in the essential oil of Schizonepeta annua pall schischk using high performance thin layer chromatography direct bioautography and gas chromatography quadrupole time of flight mass spectrometry
Jpc-journal of Planar Chromatography-modern Tlc, 2019Co-Authors: Xi Bai, Haji Akber Aisa, Maitinuer MaiwulanjiangAbstract:Schizonepeta annua (Pall.) Schischk. is an endemic annual plant from the Lamiaceae family and it has been employed to cure tra-cheitis in traditional herbal medicine. Its essential oil exhibited a strong antimicrobial and antioxidative effect. Next, high-performance thin-layer chromatography—bioautography was applied for investigation of the bioactive compounds of S. annua, and gas chromatography—quadrupole time-of-flight mass spectrometry was used to perform subsequent targeted identification of compounds. Three active components were characterized, and two of them were tentatively identified as thymol and carvacrol. S. annua has the potential to be a good alternative for synthetic disinfectants and antioxidants.
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qualitative analysis of Schizonepeta annua pall schischk essential oil by gas chromatography quadrupole time of flight mass spectrometry
European Journal of Mass Spectrometry, 2018Co-Authors: Xi Bai, Jumai Maitusong, Bakri Mahinur, Haji Akber Aisa, Maitinuer MaiwulanjiangAbstract:In this study, a method for the qualitative analysis of small molecular compounds in Schizonepeta annua (Pall.) Schischk essential oil was established based on gas chromatography-quadrupole time-of-flight mass spectrometry. In addition to an automated search of the NIST library, the identification of oxygenated monoterpenes, phenolic esters, and phenolic compounds was achieved by two additional strategies. One strategy involved comparing the relative errors of accurate masses measured for ions in the experimental spectra with those calculated for fragments identified from the NIST database of candidate matches. The second strategy involved combination of the product ion scans and positive chemical ionisation spectra for structural elucidation. Overall, 95.45% of the total essential oil volatile chemical content of Schizonepeta annua (Pall.) Schischk was identified, with phenolic monoterpenes dominating.
Kyungsik Song - One of the best experts on this subject based on the ideXlab platform.
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beneficial effects of β sitosterol on glucose and lipid metabolism in l6 myotube cells are mediated by amp activated protein kinase
Biochemical and Biophysical Research Communications, 2008Co-Authors: Seunglark Hwang, Hebok Song, Hyunhee Jung, Dongkyu Choi, Kyungsik SongAbstract:Abstract AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. β-Sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, β-sitosterol significantly increased phosphorylation of the AMPKα subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, β-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, β-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that β-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of β-sitosterol on glucose and lipid metabolism.
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beneficial effects of beta sitosterol on glucose and lipid metabolism in l6 myotube cells are mediated by amp activated protein kinase
Biochemical and Biophysical Research Communications, 2008Co-Authors: Seunglark Hwang, Hebok Song, Kyungsik Song, Hyunhee Jung, Dongkyu Choi, Hana Kim, Jieun Kim, Jongmoon Hur, Jaiyoul Lee, Taelin HuhAbstract:AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. Beta-sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, beta-sitosterol significantly increased phosphorylation of the AMPKalpha subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, beta-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, beta-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that beta-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of beta-sitosterol on glucose and lipid metabolism.
Maitinuer Maiwulanjiang - One of the best experts on this subject based on the ideXlab platform.
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spectrum effect relationship between gc qtof ms fingerprint and antioxidant anti inflammatory activities of Schizonepeta tenuifolia essential oil
Biomedical Chromatography, 2021Co-Authors: Xi Bai, Haji Akber Aisa, Liu Liu, Junping Zhang, Li Chen, Maitinuer MaiwulanjiangAbstract:Schizonepeta tenuifolia (Benth.) Briq (S. tenuifolia), a traditional Chinese medicine, is an annual herbaceous plant that is widely distributed in China, Japan, and Korea. The essential oil (EO) of S. tenuifolia has antioxidant and anti-inflammatory effects. However, the components contributing to its antioxidant and anti-inflammatory activities remain unclear. This study was aimed at investigating the spectrum-effect relationship between gas chromatography-mass spectrometry (GC-MS) fingerprint and the antioxidant and anti-inflammatory effects of S. tenuifolia EO. Herein, the fingerprints of EO from ten batches of S. tenuifolia from various sources were established using GC-MS, and the antioxidant and anti-inflammatory bioactivities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) inhibitory assays, respectively. Finally, 13 common peaks were identified from ten batches of S. tenuifolia, by searching against the standard mass spectra in NIST 14 and comparing the literature retention index. The different sources of S. tenuifolia EO exhibit mild antioxidant activities and significant anti-inflammatory effects. Specifically, menthone (peak 3), isomenthone (peak 4), pulegone (peak 7), piperitone (peak 8), and β-caryophyllene (peak 11) might be the dominant constituents responsible for the antioxidant and anti-inflammatory activities of S. tenuifolia EO. This method may provide a time-saving, convenient way to screen the potential effective components of S. tenuifolia EO.
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detection of antibacterial and antioxidant compounds in the essential oil of Schizonepeta annua pall schischk using high performance thin layer chromatography direct bioautography and gas chromatography quadrupole time of flight mass spectrometry
Jpc-journal of Planar Chromatography-modern Tlc, 2019Co-Authors: Xi Bai, Haji Akber Aisa, Maitinuer MaiwulanjiangAbstract:Schizonepeta annua (Pall.) Schischk. is an endemic annual plant from the Lamiaceae family and it has been employed to cure tra-cheitis in traditional herbal medicine. Its essential oil exhibited a strong antimicrobial and antioxidative effect. Next, high-performance thin-layer chromatography—bioautography was applied for investigation of the bioactive compounds of S. annua, and gas chromatography—quadrupole time-of-flight mass spectrometry was used to perform subsequent targeted identification of compounds. Three active components were characterized, and two of them were tentatively identified as thymol and carvacrol. S. annua has the potential to be a good alternative for synthetic disinfectants and antioxidants.
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qualitative analysis of Schizonepeta annua pall schischk essential oil by gas chromatography quadrupole time of flight mass spectrometry
European Journal of Mass Spectrometry, 2018Co-Authors: Xi Bai, Jumai Maitusong, Bakri Mahinur, Haji Akber Aisa, Maitinuer MaiwulanjiangAbstract:In this study, a method for the qualitative analysis of small molecular compounds in Schizonepeta annua (Pall.) Schischk essential oil was established based on gas chromatography-quadrupole time-of-flight mass spectrometry. In addition to an automated search of the NIST library, the identification of oxygenated monoterpenes, phenolic esters, and phenolic compounds was achieved by two additional strategies. One strategy involved comparing the relative errors of accurate masses measured for ions in the experimental spectra with those calculated for fragments identified from the NIST database of candidate matches. The second strategy involved combination of the product ion scans and positive chemical ionisation spectra for structural elucidation. Overall, 95.45% of the total essential oil volatile chemical content of Schizonepeta annua (Pall.) Schischk was identified, with phenolic monoterpenes dominating.
Seunglark Hwang - One of the best experts on this subject based on the ideXlab platform.
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beneficial effects of β sitosterol on glucose and lipid metabolism in l6 myotube cells are mediated by amp activated protein kinase
Biochemical and Biophysical Research Communications, 2008Co-Authors: Seunglark Hwang, Hebok Song, Hyunhee Jung, Dongkyu Choi, Kyungsik SongAbstract:Abstract AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. β-Sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, β-sitosterol significantly increased phosphorylation of the AMPKα subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, β-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, β-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that β-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of β-sitosterol on glucose and lipid metabolism.
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beneficial effects of beta sitosterol on glucose and lipid metabolism in l6 myotube cells are mediated by amp activated protein kinase
Biochemical and Biophysical Research Communications, 2008Co-Authors: Seunglark Hwang, Hebok Song, Kyungsik Song, Hyunhee Jung, Dongkyu Choi, Hana Kim, Jieun Kim, Jongmoon Hur, Jaiyoul Lee, Taelin HuhAbstract:AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. Beta-sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, beta-sitosterol significantly increased phosphorylation of the AMPKalpha subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, beta-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, beta-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that beta-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of beta-sitosterol on glucose and lipid metabolism.
