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A D Barth - One of the best experts on this subject based on the ideXlab platform.
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the seasonal fertility of north american bison bison bison bulls
Animal Reproduction Science, 2007Co-Authors: L Helbig, Murray R Woodbury, J C Haigh, J Collins, A D BarthAbstract:Abstract The objective of this study was to determine the presence and magnitude of seasonal fluctuations in Semen quality and other reproductive indices in bison bulls. Testicles from 288 commercially slaughtered bison bulls were collected monthly over a 1-year period. Carcass and testicle weight were determined and measurements of seminiferous tubule lumen, diameter, and epithelial thickness were made. Sperm cell morphology and defects were described and quantified using epididymal Semen from each testicle. Twenty-one Plains ( Bison bison bison ) and Wood bison ( Bison bison athabascae ) breeding bulls, averaging 6.0 years of age (range 2.5–8.0), from three farms were selected for Semen Collection and evaluation on the basis of producer co-operation. Semen was collected by electro-ejaculation on four seasonal occasions during a 12-month period. Ejaculate quality was judged on the basis of volume, density, gross and individual motility, morphology, live/dead ratio, and concentration. Sperm cell morphologies were evaluated microscopically and classified according to criteria used for bovine Semen. Fecal testosterone was measured at each Semen Collection using a commercial competitive binding radioimmunoassay. There was an increase in carcass weights over the study period and testis weights were moderately correlated ( r = 0.44) with carcass weights. However, mean testes weights were heavier ( P P P P
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comparison of electroejaculation and transrectal massage for Semen Collection in range and yearling feedlot beef bulls
Animal Reproduction Science, 2005Co-Authors: Colin W Palmer, L Soderquist, Andres A Arteaga, Leonardo F C Brito, Y Persson, A D BarthAbstract:Abstract Two experiments were conducted to compare electroejaculation (EE) and transrectal massage (RM) of the ampullary region for Semen Collection from beef bulls, and to determine the effect of Semen Collection method on Semen traits. In experiment 1, Semen was collected either by EE or RM randomly assigned on an alternate basis in 137 range beef bulls unaccustomed to being handled. The maximum time allowed for RM was 4 min and if no Semen was obtained, EE was used. In experiment 2, Semen was collected from 39 yearling feedlot beef bulls that were accustomed to being handled, by RM followed immediately by EE. The maximum time allowed for Semen Collection by both methods was 4 min. In both experiments, sperm concentration, percent of progressively motile sperm, percent of sperm staining alive, and sperm morphology were determined. In experiment 1, RM resulted in fewer ( P P P P P P P
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use of internal artificial vaginas for breeding soundness evaluation in range bulls an alternative for electroejaculation allowing observation of sex drive and mating ability
Animal Reproduction Science, 2004Co-Authors: A D Barth, Andres A Arteaga, Leonardo F C Brito, Colin W PalmerAbstract:Abstract The objective of this study was to test the efficacy of an inexpensive, reusable internal artificial vagina (IAV) developed for breeding soundness evaluation of range beef bulls. In addition, sexual behavior during Semen Collection by IAV was compared to behavior during pasture breeding. Breeding soundness exams (BSEs) were conducted on 165 bulls in two consecutive years (96 in Year 1 and 69 in Year 2). In Year 1, BSEs were done twice in all bulls, once by a conventional protocol using electroejaculation (EEJ), followed by the IAV method, one week later. In Year 2, all BSEs were done on one day; 69 bulls by the IAV method followed by EEJ in 21 bulls that failed to serve the IAV. For Semen Collection using an IAV, mount cows were restrained in breeding crates and an IAV was inserted into the vagina just beyond the depth of the vestibular sphincter. After each copulation, the IAV was replaced for the next bull to be tested. Semen Collection by IAV was successful for all bulls that mounted and penetrated cows during the testing period (54.3 and 69.6% of the bulls served the cows with IAVs in Year 1 and 2, respectively). Semen was collected successfully by EEJ from all bulls in both years. Differences were observed between Semen Collection methods in Semen volume and percentage of sperm staining alive; however, the differences were opposite in Year 1 and 2 and, therefore, were probably due to natural variations in time and within bull rather than the method of Semen Collection. Semen Collection by IAV allowed the detection of problems that prevented copulation in 8 bulls (4.8%) that were determined to be satisfactory potential breeders when Semen was collected by EEJ. In Year 1, breeding observations were made at pasture for 15 bulls that served, and 15 bulls that did not serve cows with an IAV. Bulls that did not serve the IAV during the test period had fewer mounts, attempts to mount, and completed services at pasture than bulls that had served the IAV, indicating that such bulls are likely to be less efficient breeders. In conclusion, IAVs might be used as an alternative to EEJ for breeding soundness evaluation in about 50–70% of range bulls. Semen Collection by IAV also allows observation of sex drive and mating ability thus improves the ability to select sound breeding bulls.
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use of oxytocin and cloprostenol to facilitate Semen Collection by electroejaculation or transrectal massage in bulls
Animal Reproduction Science, 2004Co-Authors: C W Palmer, Cheryl L Waldner, Leonardo F C Brito, S D Amundson, A D BarthAbstract:Abstract Two experiments were conducted with 24 bulls in which Semen Collection was attempted by transrectal massage (RM) and electroejaculation (EE). In experiment 1, bulls received the following treatments on successive Semen Collection days: saline 10 min prior to electroejaculation (control); saline 10 min prior to 2 min of transrectal massage followed by electroejaculation; cloprostenol (CLO) 10 min prior to 2 min of transrectal massage followed by electroejaculation; oxytocin (OXY) 10 min prior to 2 min of transrectal massage followed by electroejaculation. Transrectal massage consisted of general, back and forth motion over the ampullae, prostate and urethra with a flattened hand. In experiment 2, bulls received saline (control), oxytocin, or cloprostenol 10 min before attempting Semen Collection by transrectal massage. Massage was applied specifically to the ampullae for a maximum of 5 min or until a Semen sample was obtained. Electroejaculation was attempted in all bulls following transrectal massage. In experiment 1, Semen was obtained in Oxytocin treatment increased the time to penile protrusion during electroejaculation in experiment 1 and during massage in experiment 2. In experiment 1, oxytocin decreased the time to Semen emission and tended to decrease the number of electroejaculation stimuli to Semen emission. Cloprostenol treatment, in experiment 1, resulted in an increased number of electroejaculation stimuli to penile protrusion, but did not affect the number of stimuli required for Semen emission. Massage of the ampullae prior to electroejaculation reduced both the time to Semen emission and the number of electroejaculation stimuli required for Semen emission. Transrectal massage of the ampullae was very effective in this experiment for producing Semen emission, but quantity of Semen samples was less than for electroejaculation. The usefulness of transrectal massage for Semen Collection in breeding soundness evaluations needs to be investigated further under field conditions.
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Use of internal artificial vaginas for breeding soundness evaluation in range bulls: an alternative for electroejaculation allowing observation of sex drive and mating ability.
Animal reproduction science, 2004Co-Authors: A D Barth, Andres A Arteaga, Leonardo F C Brito, Colin W PalmerAbstract:The objective of this study was to test the efficacy of an inexpensive, reusable internal artificial vagina (IAV) developed for breeding soundness evaluation of range beef bulls. In addition, sexual behavior during Semen Collection by IAV was compared to behavior during pasture breeding. Breeding soundness exams (BSEs) were conducted on 165 bulls in two consecutive years (96 in Year 1 and 69 in Year 2). In Year 1, BSEs were done twice in all bulls, once by a conventional protocol using electroejaculation (EEJ), followed by the IAV method, one week later. In Year 2, all BSEs were done on one day; 69 bulls by the IAV method followed by EEJ in 21 bulls that failed to serve the IAV. For Semen Collection using an IAV, mount cows were restrained in breeding crates and an IAV was inserted into the vagina just beyond the depth of the vestibular sphincter. After each copulation, the IAV was replaced for the next bull to be tested. Semen Collection by IAV was successful for all bulls that mounted and penetrated cows during the testing period (54.3 and 69.6% of the bulls served the cows with IAVs in Year 1 and 2, respectively). Semen was collected successfully by EEJ from all bulls in both years. Differences were observed between Semen Collection methods in Semen volume and percentage of sperm staining alive; however, the differences were opposite in Year 1 and 2 and, therefore, were probably due to natural variations in time and within bull rather than the method of Semen Collection. Semen Collection by IAV allowed the detection of problems that prevented copulation in 8 bulls (4.8%) that were determined to be satisfactory potential breeders when Semen was collected by EEJ. In Year 1, breeding observations were made at pasture for 15 bulls that served, and 15 bulls that did not serve cows with an IAV. Bulls that did not serve the IAV during the test period had fewer mounts, attempts to mount, and completed services at pasture than bulls that had served the IAV, indicating that such bulls are likely to be less efficient breeders. In conclusion, IAVs might be used as an alternative to EEJ for breeding soundness evaluation in about 50-70% of range bulls. Semen Collection by IAV also allows observation of sex drive and mating ability thus improves the ability to select sound breeding bulls.
K Kamau - One of the best experts on this subject based on the ideXlab platform.
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Semen Collection and seminal characteristics of the australian saltwater crocodile crocodylus porosus
Aquaculture, 2014Co-Authors: S D Johnston, J Lever, R Mcleod, E Qualischefski, Motoharu Oishi, C Omanga, M Leitner, R Price, L Barker, K KamauAbstract:The development of a reliable non-invasive method of crocodile Semen Collection is fundamental for assessing male fertility and for implementing the benefits of artificial insemination for reproductive and genetic management of farmed populations and the captive breeding of endangered species. Following an initial description of male reproductive anatomy in the saltwater crocodile (Crocodylus porosus), this study developed a successful, reliable method of Semen Collection using cloacal digital manipulation of the terminal segment of the ductus deferens. Semen was recovered from 30 of 31 Collection attempts from a total of 24 sedated males ranging from 197cm to 400cm in body length. Seminal characteristics (including abnormal sperm morphology) and bacteriology were documented for the first time. Mean (±SEM) seminal volume, pH, osmolality, sperm concentration, percentage of motile sperm and the percentage of sperm with an intact membrane measured 0.91±0.16mL, 7.3±0.1, 335.5±9.0mOsmkg-1, 2.29±0.26×109, 50.7±4.2%, and 79.9±3.6%, respectively. Sperm abnormalities included macro and microcephalic nuclei, teratoid spermatozoa, loose heads and a range of abnormal flagella. Most Semen samples contained spermatozoa with what are presumed to be cytoplasmic droplets but the confirmation and then significance of this phenomenon as a sperm maturational pathology require further validation. In an attempt to develop targeted antibiotics for use in Semen diluents, microflora of the penile shaft, sulcus and Semen of a subset of crocodiles was analysed for culture and sensitivity. While a diverse range of bacteria were identified, the majority were sensitive to gentamicin. This study represents the first step towards assessment of breeding soundness examination and the use of artificial insemination technology in the saltwater crocodile industry.
Dondin Sajuthi - One of the best experts on this subject based on the ideXlab platform.
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The use of transrectal massage combined with artificial vagina as Semen Collection in javan banteng (Bos javanicus) bull
E3S Web of Conferences, 2020Co-Authors: Vincentia Trisna Yoelinda, Raden Iis Arifiantini, Muhammad Agil, Dedi R. Setiadi, Yohana Tri Hastuti, Jansen Manansang, Dondin SajuthiAbstract:Semen Collection in wild animals may be challenging for some methods such as electro ejaculator may cause pain and distress to the animals. Transrectal massage has been widely used in domestic and wild animals Semen Collection. Even though an artificial vagina may provide a condition that mimics the female reproductive tract and known to provide better results in some species, the application may face obstacles in the field. This study was conducted to evaluate the possibility of modified Semen Collection in banteng bull by combining the transrectal massage method followed by the use of an artificial vagina. The bull was habituated and trained for Semen Collection in chute under veterinarian supervision in Taman Safari Indonesia, Cisarua, Bogor, West Java. Semen samples from one Javan banteng bull were collected once a week. Banteng bull showed stud and penile protrusion during Semen Collection. Watery Semen with volume ranged from 12 ml were successfully collected, with sperm progressive motility ranged from 25%-40%. Our study also showed sperm abnormalities comprising 18.11% abnormal sperm heads, 28.82% abnormal sperm tails and 0.94% teratoid form. The results demonstrated the possibility of this modified method as an alternative procedure for Semen Collection in banteng bull which may also be useful for other wild animals in field conditions
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The use of transrectal massage combined with artificial vagina as Semen Collection in javan banteng (
'EDP Sciences', 2020Co-Authors: Vincentia Trisna Yoelinda, Raden Iis Arifiantini, Muhammad Agil, Dedi R. Setiadi, Yohana Tri Hastuti, Jansen Manansang, Dondin SajuthiAbstract:Semen Collection in wild animals may be challenging for some methods such as electro ejaculator may cause pain and distress to the animals. Transrectal massage has been widely used in domestic and wild animals Semen Collection. Even though an artificial vagina may provide a condition that mimics the female reproductive tract and known to provide better results in some species, the application may face obstacles in the field. This study was conducted to evaluate the possibility of modified Semen Collection in banteng bull by combining the transrectal massage method followed by the use of an artificial vagina. The bull was habituated and trained for Semen Collection in chute under veterinarian supervision in Taman Safari Indonesia, Cisarua, Bogor, West Java. Semen samples from one Javan banteng bull were collected once a week. Banteng bull showed stud and penile protrusion during Semen Collection. Watery Semen with volume ranged from 12 ml were successfully collected, with sperm progressive motility ranged from 25%-40%. Our study also showed sperm abnormalities comprising 18.11% abnormal sperm heads, 28.82% abnormal sperm tails and 0.94% teratoid form. The results demonstrated the possibility of this modified method as an alternative procedure for Semen Collection in banteng bull which may also be useful for other wild animals in field condition
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FA-5 Semen Characteristics of Banteng (Bos javanicus) Collected by Electroejaculation Method
2018Co-Authors: Vincentia Trisna Yoelinda, Muhammad Agil, Dedi R. Setiadi, Yohana Tri Hastuti, Jansen Manansang, Iis Afriantini, Tuty Laswardi Yusuf, Dondin SajuthiAbstract:Banteng (Bos javanicus) is listed as endangered species in IUCN Redlist. Only few thousand wild banteng survive and their numbers are decreasing as a result of hunting and the loss of habitat due to deforestation and conversion to agricultural land. One of the conservation measures is to preserve this species in ex-situ breeding center. The breeding centre with the largest number of individuals is Ragunan Zoo in Jakarta, with 21 animals. Despite those numbers of individuals available, the sex ratio was far from ideal at 16 males and five females. While in Taman Safari Prigen, there were with 16 individuals and a sex ratio of five males and 11 females [1] Since number of males are usually less than females, and also to avoid inbreeding in order to keep high heterozygosity population, therefore, assisted reproductive technology such as artificial insemination is considered to be necessary.For artificial insemination, Semen Collection sufficient quantitiy and quality of Semen from males are needed. Thus, best Semen Collection method should be considered. Though transrectal massage procedure does not require the male to be anesthetized prior to Semen Collection, Semen collected by this technique contains urine contamination. Besides, volume of Semen sample obtained by transrectal massage is lesser compared to electroejaculation. Electroejaculation is also suitable for wild animal because in this particular Semen Collection procedure, males are not required to be trained.The information about Semen characteristics of banteng bulls collected by electroejaculation is less known. To date, study done by Johnston et al. (2002) is the only available data. That study only provided data from one Semen sample from one 4-year-old banteng bull located at Western Plains Zoo [2]. Moreover such volume parameter and consistency was not reported from that study. Thus, this study is aimed to provide more reliable data from larger number of Semen samples collected by electroejaculation and to add information to previous data that are availabe.
Aziz Abdul Malek - One of the best experts on this subject based on the ideXlab platform.
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Digital massage for Semen Collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
Aquaculture, 2018Co-Authors: Wan-nor Fitri, H. Wahid, Putra Tengku Rinalfi, Y. Rosnina, Dana Raj, Yawah Donny, Latip Qayyum, Aziz Abdul MalekAbstract:Abstract Study in Semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the Semen Collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of Semen Collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for Semen Collection. The Collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 × 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen Collection by digital manipulation of the penis was adequate in obtaining Semen and the Semen characteristics of the estuarine crocodile are described.
S D Johnston - One of the best experts on this subject based on the ideXlab platform.
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Semen Collection and seminal characteristics of the australian saltwater crocodile crocodylus porosus
Aquaculture, 2014Co-Authors: S D Johnston, J Lever, R Mcleod, E Qualischefski, Motoharu Oishi, C Omanga, M Leitner, R Price, L Barker, K KamauAbstract:The development of a reliable non-invasive method of crocodile Semen Collection is fundamental for assessing male fertility and for implementing the benefits of artificial insemination for reproductive and genetic management of farmed populations and the captive breeding of endangered species. Following an initial description of male reproductive anatomy in the saltwater crocodile (Crocodylus porosus), this study developed a successful, reliable method of Semen Collection using cloacal digital manipulation of the terminal segment of the ductus deferens. Semen was recovered from 30 of 31 Collection attempts from a total of 24 sedated males ranging from 197cm to 400cm in body length. Seminal characteristics (including abnormal sperm morphology) and bacteriology were documented for the first time. Mean (±SEM) seminal volume, pH, osmolality, sperm concentration, percentage of motile sperm and the percentage of sperm with an intact membrane measured 0.91±0.16mL, 7.3±0.1, 335.5±9.0mOsmkg-1, 2.29±0.26×109, 50.7±4.2%, and 79.9±3.6%, respectively. Sperm abnormalities included macro and microcephalic nuclei, teratoid spermatozoa, loose heads and a range of abnormal flagella. Most Semen samples contained spermatozoa with what are presumed to be cytoplasmic droplets but the confirmation and then significance of this phenomenon as a sperm maturational pathology require further validation. In an attempt to develop targeted antibiotics for use in Semen diluents, microflora of the penile shaft, sulcus and Semen of a subset of crocodiles was analysed for culture and sensitivity. While a diverse range of bacteria were identified, the majority were sensitive to gentamicin. This study represents the first step towards assessment of breeding soundness examination and the use of artificial insemination technology in the saltwater crocodile industry.
