The Experts below are selected from a list of 1308 Experts worldwide ranked by ideXlab platform
Alexander Plyusnin - One of the best experts on this subject based on the ideXlab platform.
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genetic analyses of Seoul hantaVirus genome recovered from rats rattus norvegicus in the netherlands unveils diverse routes of spread into europe
Journal of Medical Virology, 2019Co-Authors: Jiaxin Ling, Alexander Plyusnin, Angelina Plyusnina, Jenny Vernercarlsson, Per Eriksson, Mare Lohmus, Josef D Jarhult, Frank Van De Goot, Åke LundkvistAbstract:Seoul Virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban ...
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cross species transmission in the speciation of the currently known murinae associated hantaViruses
Journal of Virology, 2012Co-Authors: Xiandan Lin, Xiaohe Zhang, Alexander Plyusnin, Yi Chen, Wen Wang, Wenping Guo, Jianguang Xing, Shengze Chen, Yongzhen ZhangAbstract:To gain more insight into the phylogeny of Dabieshan Virus (DBSV), carried by Niviventer confucianus and other Murinae-associated hantaViruses, genome sequences of novel variants of DBSV were recovered from Niviventer rats trapped in the mountainous areas of Wenzhou, China. Genetic analyses show that all known genetic variants of DBSV, including the ones identified in this study, are distinct from other Murinae-associated hantaViruses. DBSV variants show geographic clustering and high intraspecies diversity. The data suggest that DBSV is a distinct species in the genus HantaVirus. Interestingly, DBSV shows the highest sequence identity to Hantaan Virus (HTNV), with a >7% difference in the sequences of the N, GPC, and L proteins, while N. confucianus is more closely related to Rattus norvegicus (the host of Seoul Virus [SEOV]) than to Apodemus agrarius (the host of HTNV and Saaremaa Virus [SAAV]). Further genetic analyses of all known Murinae-associated hantaViruses (both established and tentative species) show that many of them, including DBSV, may have originated from host switching. The estimation of evolutionary rates and divergence time supports the role of cross-species transmission in the evolution of Murinae-associated hantaViruses. The detection of positive selection suggests that genetic drift may contribute to the speciation of Murinae-associated hantaViruses and that adaptation has a role as well.
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genetic characterization of Seoul hantaVirus originated from norway rats rattus norvegicus captured in belgium
Journal of Medical Virology, 2012Co-Authors: Angelina Plyusnina, Paul Heyman, Kristof Baert, Jan Stuyck, C Cochez, Alexander PlyusninAbstract:HantaViruses (genus HantaVirus, family Bunyaviridae) cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantaVirus (cardio)pulmonary syndrome (HCPS) in the Americas. So far, in Europe, four pathogenic hantaViruses have been found, often in co-circulation: Puumala Virus (PUUV), Dobrava Virus (DOBV), Saaremaa Virus (SAAV), and Seoul Virus (SEOV). Of those, only PUUV was found in Belgium. Recently, in our search for hantaViruses in the Flanders region of Belgium we collected genetic and serological evidence for the presence of SEOV Virus in local brown rats. In this article, the results of (phylo)genetic analysis of wild-type SEOV strain from the Flanders are presented. The analysis based on the complete S segment sequence and also partial M- and L-segment sequences revealed that the Belgian SEOV strain was related most closely to strains from France, Indonesia, Japan, Singapore, Cambodia (those associated with the species Rattus norvegicus) and Vietnam. Such a clustering was in perfect agreement with the results of direct sequence comparison and suggested the same evolutionary history for all three genome segments of the Belgian SEOV strain (i.e., no reassortment of genome segments). So far, SEOV has been found in two European countries, France and Belgium, and there is every reason to believe that the area of the Virus distribution in Europe is not restricted to those countries. J. Med. Virol. 84: 1298–1303, 2012. © 2012 Wiley Periodicals, Inc.
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hantaVirus infections in humans and animals china
Emerging Infectious Diseases, 2010Co-Authors: Yongzhen Zhang, Yang Zou, Alexander PlyusninAbstract:Hemorrhagic fever with renal syndrome (HFRS) is a serious public health problem in the People’s Republic of China. Although 7 sero/genotypes of hantaViruses have been found in rodents, only Hantaan Virus (carried by Apodemus agrarius mice) and Seoul Virus (carried by Rattus norvegicus rats) reportedly cause disease in humans. During 1950–2007, a total of 1,557,622 cases of HFRS in humans and 46,427 deaths (3%) were reported in China. HFRS has been reported in 29 of 31 provinces in China. After implementation of comprehensive preventive measures, including vaccination, in the past decade in China, incidence of HFRS has dramatically decreased; only 11,248 HFRS cases were reported in 2007. Mortality rates also declined from the highest level of 14.2% in 1969 to ≈1% during 1995–2007. However, the numbers of HFRS cases and deaths in China remain the highest in the world.
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co circulation of three pathogenic hantaViruses puumala dobrava and saaremaa in hungary
Journal of Medical Virology, 2009Co-Authors: Angelina Plyusnina, Antti Vaheri, Åke Lundkvist, Emoke Ferenczi, Gabor R Racz, Kirill Nemirov, Alexander PlyusninAbstract:HantaViruses (Bunyaviridae) cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantaVirus (cardio)pulmonary syndrome (HCPS) in the Americas. HFRS is caused by Hantaan Virus (HTNV), Seoul Virus (SEOV), Dobrava Virus (DOBV), Saaremaa Virus (SAAV), and Puumala Virus (PUUV). Of those, only HTNV is not present in Europe. In recent years, hantaViruses, described in other parts of Europe, were also detected at various locations in Hungary. To study the genetic properties of Hungarian hantaViruses in detail, sequences of the viral S and M segments were recovered from bank voles (Myodes glareolus), yellow-necked mice (Apodemus flavicollis), and striped field mice (Apodemus agrarius) trapped in the Transdanubian region. As expected, the sequences recovered belonged, respectively, to PUUV (two strains), DOBV (one strain), and SAAV (one strain). On phylogenetic trees two new Hungarian PUUV strains located within the well- supported Alpe-Adrian (ALAD) genetic lineage that included also Austrian, Slovenian, and Croatian strains. Analysis of the Hungarian SAAV and DOBV genetic variants showed host-specific clustering and also geographical clustering within each of these hantaVirus species. Hungarian SAAV and DOBV strains were related most closely to strains from Slovenia (Prekmurje region). This study confirms that multiple hantaViruses can co-circulate in the same locality and can be maintained side-by-side in different rodent species. J. Med. Virol. 81:2045–2052, 2009. © 2009 Wiley-Liss, Inc.
Sabra L Klein - One of the best experts on this subject based on the ideXlab platform.
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A Case Study of Two Rodent-Borne Viruses: Not Always the Same Old Suspects
Frontiers Media S.A., 2019Co-Authors: James E. Childs, Sabra L Klein, Gregory E GlassAbstract:Two Old World rodents, house mice (Mus musculus) and Norway rats (Rattus norvegicus), were introduced into and established populations on every continent, save Antarctica. With their travels, they concomitantly introduced several zoonotic agents capable of causing human diseases. Two Viruses—Lymphocytic choriomeningitis Virus (LCMV; genus ArenaVirus with mice) and Seoul Virus (SEOV; genus HantaVirus with rats)—can cause chronic infections within their respective rodent hosts, resulting in persistent or life-long sporadic shedding of Virus through secreta and excreta. Although the prevalence of infection within their wild rodent hosts can exceed 25% among mice infected with LCMV and 50% among rats infected with SEOV, acute human disease resulting from direct transmission from wild rodents is rarely reported even though both species live in close coexistence with humans. The usual “classic” zoonotic cycle of transmission from wild rodent reservoirs to humans now includes multiple unusual/unexpected routes. The largest described outbreaks of human disease caused by these Viruses are linked to pet rodents. A novel reservoir host, the golden hamster, has supplanted house mice as the major source of LCMV infection, and SEOV outbreaks are linked to fancy rats kept as pets. Following LCMV, and to a lesser extent SEOV, outbreaks or infections associated with lab animals and/or cultured tissues derived from mice and hamsters have led to hundreds of cases of LCMV among laboratory workers, and SEOV has been detected among cell-cultured tissues. Additionally, LCMV is now a recognized source of severe congenital disease and is the unexpected source of severe and often fatal disease among solid organ recipients. Although the extensive usual and unusual routes of LCMV infection are exceptional there are many parallels with SEOV emergence
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Seoul Virus-Infected Rat Lung Endothelial Cells and Alveolar Macrophages Differ in Their Ability To Support Virus Replication and
2016Co-Authors: Induce Regulatory, Cell T Phenotypes, Sabra L KleinAbstract:HantaViruses cause a persistent infection in reservoir hosts that is attributed to the upregulation of regulatory responses and downregulation of proinflammatory responses. To determine whether rat alveolar macrophages (AMs) and lungmicrovascular endothelial cells (LMVECs) support Seoul Virus (SEOV) replication and contribute to the induction of an environment that po-larizes CD4 T cell differentiation toward a regulatory T (Treg) cell phenotype, cultured primary rat AMs and LMVECs were mock infected or infected with SEOV and analyzed for viral replication, cytokine and chemokine responses, and expression of cell surface markers that are related to T cell activation. Allogeneic CD4 T cells were cocultured with SEOV-infected or mock-infected AMs or LMVECs and analyzed for helper T cell (i.e., Treg, Th17, Th1, and Th2) marker expression and Treg cell fre-quency. SEOV RNA and infectious particles in culture media were detected in both cell types, but at higher levels in LMVECs than in AMs postinfection. Expression of Ifn, Ccl5, and Cxcl10 and surface major histocompatibility complex class II (MHC-II) andMHC-I was not altered by SEOV infection in either cell type. SEOV infection significantly increased TgfmRNA in AMs and the amount of programmed cell death 1 ligand 1 (PD-L1) in LMVECs. SEOV-infected LMVECs, but not AMs, induced a signifi-cant increase in Foxp3 expression and Treg cell frequency in allogeneic CD4 T cells, which was Virus replication and cell contact dependent. These data suggest that in addition to supporting viral replication, AMs and LMVECs play distinct roles in hantavi-rus persistence by creating a regulatory environment through increased Tgf, PD-L1, and Treg cell activity. HantaViruses are negative-strand RNA Viruses (family Bunya-viridae) that are maintained in the environment by causin
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2001 Sex differences in immune responses and viral shedding following Seoul Virus infection in Norway rats
2015Co-Authors: Sabra L Klein, Brian H Bird, E. Glass, The Harry W. FeinstoneAbstract:Abstract. In the field, male rodents are more frequently infected with hantaViruses than females. This study examined whether patterns of immune responses against hantaVirus differed between the sexes. Male and female Long Evans rats (Rattus norvegicus) were inoculated with Seoul Virus, and antibody and cytokine responses, as well as Virus shedding were assessed. Males were more likely to shed Virus in saliva, to shed Virus through multiple routes (saliva, urine, and feces), and to have viral RNA in the spleen than females. Anti-Seoul Virus IgG responses were higher in males than females. In both sexes, splenic IFN and IL-4 production increased following infection. After infection, males had higher Th1 immune responses (i.e., IgG2a, IFN, and IL-2) than females; in contrast, Th2 immune responses (i.e., IgG1, IL-4, and IL-10) were similar between the sexes. These data suggest that immune responses to Seoul Virus differ between the sexes
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Seoul Virus infected rat lung endothelial cells and alveolar macrophages differ in their ability to support Virus replication and induce regulatory t cell phenotypes
Journal of Virology, 2012Co-Authors: Sabra L KleinAbstract:ABSTRACT HantaViruses cause a persistent infection in reservoir hosts that is attributed to the upregulation of regulatory responses and downregulation of proinflammatory responses. To determine whether rat alveolar macrophages (AMs) and lung microvascular endothelial cells (LMVECs) support Seoul Virus (SEOV) replication and contribute to the induction of an environment that polarizes CD4 + T cell differentiation toward a regulatory T (Treg) cell phenotype, cultured primary rat AMs and LMVECs were mock infected or infected with SEOV and analyzed for viral replication, cytokine and chemokine responses, and expression of cell surface markers that are related to T cell activation. Allogeneic CD4 + T cells were cocultured with SEOV-infected or mock-infected AMs or LMVECs and analyzed for helper T cell (i.e., Treg, Th17, Th1, and Th2) marker expression and Treg cell frequency. SEOV RNA and infectious particles in culture media were detected in both cell types, but at higher levels in LMVECs than in AMs postinfection. Expression of Ifn β, Ccl5 , and Cxcl10 and surface major histocompatibility complex class II (MHC-II) and MHC-I was not altered by SEOV infection in either cell type. SEOV infection significantly increased Tgf β mRNA in AMs and the amount of programmed cell death 1 ligand 1 (PD-L1) in LMVECs. SEOV-infected LMVECs, but not AMs, induced a significant increase in Foxp3 expression and Treg cell frequency in allogeneic CD4 + T cells, which was Virus replication and cell contact dependent. These data suggest that in addition to supporting viral replication, AMs and LMVECs play distinct roles in hantaVirus persistence by creating a regulatory environment through increased Tgf β, PD-L1, and Treg cell activity.
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elevated testosterone and reduced 5 hiaa concentrations are associated with wounding and hantaVirus infection in male norway rats
Hormones and Behavior, 2007Co-Authors: Judith D Easterbrook, Gregory E Glass, Jenifer B Kaplan, Mikhail V Pletnikov, Sabra L KleinAbstract:Among rodents that carry hantaViruses, males are more likely to engage in aggression and to be infected than females. One mode of hantaVirus transmission is via the passage of Virus in saliva during wounding. The extent to which hantaViruses cause physiological changes in their rodent host that increase aggression and, therefore, Virus transmission has not been fully documented. To assess whether steroid hormones and neurotransmitters contribute to the correlation between aggression and Seoul Virus infection, Norway rats were trapped in Baltimore, Maryland and wounding, infection status, steroid hormones, and concentrations of neurotransmitters, including norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenol acetic acid (DOPAC), serotonin (5-HT), and 5-hydroxyindole-3-acetic acid (5-HIAA) in select brain regions were examined. Older males and males with high-grade wounds were more likely to have anti-Seoul Virus IgG and viral RNA in organs than either juveniles or adult males with less severe wounds. Wounded males had higher circulating testosterone, lower hypothalamic 5-HIAA, and lower NE in the amygdala than males with no wounds. Infected males had higher concentrations of testosterone, corticosterone, NE in the hypothalamus, and DOPAC in the amygdala than uninfected males, regardless of wounding status. In the present study, wounded males that were infected with Seoul Virus had elevated testosterone and reduced 5-HIAA concentrations, suggesting that these neuroendocrine mechanisms may contribute to aggression and the likelihood of transmission of hantaVirus in natural populations of male Norway rats.
Jean-marc Reynes - One of the best experts on this subject based on the ideXlab platform.
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Wild Rats, Laboratory Rats, Pet Rats: Global Seoul HantaVirus Disease Revisited
Viruses, 2019Co-Authors: Jan Clement, Jean-marc Reynes, James Leduc, Graham Lloyd, Lorraine Mcelhinney, Marc Van Ranst, Ho-wang LeeAbstract:Recent reports from Europe and the USA described Seoul orthohantaVirus infection in pet rats and their breeders/owners, suggesting the potential emergence of a "new" public health problem. Wild and laboratory rat-induced Seoul infections have, however, been described since the early eighties, due to the omnipresence of the rodent reservoir, the brown rat Rattus norvegicus. Recent studies showed no fundamental differences between the pathogenicity and phylogeny of pet rat-induced Seoul orthohantaViruses and their formerly described wild or laboratory rat counterparts. The paucity of diagnosed Seoul Virus-induced disease in the West is in striking contrast to the thousands of cases recorded since the 1980s in the Far East, particularly in China. This review of four continents (Asia, Europe, America, and Africa) puts this "emerging infection" into a historical perspective, concluding there is an urgent need for greater medical awareness of Seoul Virus-induced human pathology in many parts of the world Given the mostly milder and atypical clinical presentation, sometimes even with preserved normal kidney function, the importance of simple but repeated urine examination is stressed, since initial but transient proteinuria and microhematuria are rarely lacking.
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Seoul Virus Infection in Humans, France, 2014–2016
Emerging Infectious Diseases, 2017Co-Authors: Jean-marc Reynes, Damien Carli, Jean-baptiste Bour, Samir Boudjeltia, Anny Dewilde, Guillaume Gerbier, Timothée Nussbaumer, Véronique Jacomo, Marie-pierre Rapt, Pierre RollinAbstract:We report detection of Seoul Virus in 3 patients in France over a 2-year period. These patients accounted for 3 of the 4 Seoul Virus infections among 434 hantaVirus infections (1.7%) reported during this time. More attention should be given to this Virus in Europe where surveillance has been focused mostly on Puumala and Dobrava-Belgrade hantaViruses.
Marit Kramski - One of the best experts on this subject based on the ideXlab platform.
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Detection and typing of human pathogenic hantaViruses by real-time reverse transcription-PCR and pyrosequencing
2015Co-Authors: Marit Kramski, Boris Klempa, Helga Meisel, Georg Pauli, Detlev H. Krüger, Andreas NitscheAbstract:Background: Because the clinical course of human in-fections with hantaViruses can vary from subclinical to fatal, rapid and reliable detection of hantaViruses is essential. To date, the diagnosis of hantaVirus infection is based mainly on serologic assays, and the detection of hantaviral RNA by the commonly used reverse tran-scription (RT)-PCR is difficult because of high sequence diversity of hantaViruses and low viral loads in clinical specimens. Methods:We developed 5 real-time RT-PCR assays, 3 of which are specific for the individual European hantavi-ruses Dobrava, Puumala, or Tula Virus. Two additional assays detect the Asian species Hantaan Virus together with Seoul Virus and the American species Andes Virus together with Sin Nombre Virus. Pyrosequencing wa
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detection and typing of human pathogenic hantaViruses by real time reverse transcription pcr and pyrosequencing
Clinical Chemistry, 2007Co-Authors: Boris Klempa, Detlev H. Krüger, Helga Meisel, Georg Pauli, Marit Kramski, Andreas NitscheAbstract:Background: Because the clinical course of human infections with hantaViruses can vary from subclinical to fatal, rapid and reliable detection of hantaViruses is essential. To date, the diagnosis of hantaVirus infection is based mainly on serologic assays, and the detection of hantaviral RNA by the commonly used reverse transcription (RT)-PCR is difficult because of high sequence diversity of hantaViruses and low viral loads in clinical specimens. Methods: We developed 5 real-time RT-PCR assays, 3 of which are specific for the individual European hantaViruses Dobrava, Puumala, or Tula Virus. Two additional assays detect the Asian species Hantaan Virus together with Seoul Virus and the American species Andes Virus together with Sin Nombre Virus. Pyrosequencing was established to provide characteristic sequence information of the amplified hantaVirus for confirmation of the RT-PCR results or for a more detailed Virus typing. Results: The real-time RT-PCR assays were specific for the respective hantaVirus species and optimized to run on 2 different platforms, the LightCycler and the ABI 7900/7500. Each assay showed a detection limit of 10 copies of a plasmid containing the RT-PCR target region, and pyrosequencing was possible with 10 to 100 copies per reaction. With this assay, viral genome could be detected in 16 of 552 (2.5%) specimens of suspected hantaVirus infections of humans and mice. Conclusions: The new assays detect, differentiate, and quantify hantaViruses in clinical specimens from humans and from their natural hosts and may be useful for in vitro studies of hantaViruses.
Ho-wang Lee - One of the best experts on this subject based on the ideXlab platform.
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Wild Rats, Laboratory Rats, Pet Rats: Global Seoul HantaVirus Disease Revisited
Viruses, 2019Co-Authors: Jan Clement, Jean-marc Reynes, James Leduc, Graham Lloyd, Lorraine Mcelhinney, Marc Van Ranst, Ho-wang LeeAbstract:Recent reports from Europe and the USA described Seoul orthohantaVirus infection in pet rats and their breeders/owners, suggesting the potential emergence of a "new" public health problem. Wild and laboratory rat-induced Seoul infections have, however, been described since the early eighties, due to the omnipresence of the rodent reservoir, the brown rat Rattus norvegicus. Recent studies showed no fundamental differences between the pathogenicity and phylogeny of pet rat-induced Seoul orthohantaViruses and their formerly described wild or laboratory rat counterparts. The paucity of diagnosed Seoul Virus-induced disease in the West is in striking contrast to the thousands of cases recorded since the 1980s in the Far East, particularly in China. This review of four continents (Asia, Europe, America, and Africa) puts this "emerging infection" into a historical perspective, concluding there is an urgent need for greater medical awareness of Seoul Virus-induced human pathology in many parts of the world Given the mostly milder and atypical clinical presentation, sometimes even with preserved normal kidney function, the importance of simple but repeated urine examination is stressed, since initial but transient proteinuria and microhematuria are rarely lacking.
