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Yoichi Matsuda - One of the best experts on this subject based on the ideXlab platform.
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Slot blot hybridization of four repetitive sequences to genomic DNA of 17 species.
2019Co-Authors: Yoshinobu Uno, Chizuko Nishida, Satoshi Ishishita, Ayano Hata, Yoichi MatsudaAbstract:The fragments used for this experiment are as follows: APL-HaeIII-04 (A), AFA-HinfI-S03 (B), AFA-HinfI-L04 (C), and CCY-ApaI-05 (D). Genomic DNA used for this experiment was obtained from the following avian species of 10 orders: (1) Struthioniformes, SCA (S. camelus) and DNO (D. novaehollandiae); (2) Tinamiformes, EEL (E. elegans); (3) Galliformes, NME (N. meleagris), CJA (C. japonica), and GGA (G. gallus); (4) Anseriformes, AFA (A. fabalis), CCY (C. cygnus), and APL (A. platyrhynchos); (5) Gruiformes, GLE (G. leucogeranus); (6) Pelecaniformes, PMI (P. minor); (7) Strigiformes, BBL (B. blakistoni); (8) Accipitriformes, PHA (P. haliaetus) and NNI (N. nipalensis orientalis); (9) Psittaciformes, PAT (P. aterrimus) and AAU (A. autumnalis); and (10) Passeriformes, HRU (H. rustica). The locations of genomic DNA on membranes used for slot blot hybridization are represented at the bottom of the figures.
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Comparison of the Amino Acid Sequences of TENP among Four Avian Species.
2016Co-Authors: Keiji Kinoshita, Masaoki Tsudzuki, Takeshi Shimogiri, Hisham R. Ibrahim, Yoshizane Maeda, Yoichi MatsudaAbstract:Chicken (Gallus gallus) (AB219158), duck (Anasplatyrhynchos, Anserformes) (XP005011070), zebra finch (Taeniopygia guttata,Passeriformes) (XP012425675) and emu (Dromaius novaehollandiae, Struthioniformes) (AB556937). Asterisks indicate the amino acids that are common in all of the species. The positions of the amino acid substitutions detected in the chicken TENP are painted in gray.
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Chromosome size-correlated and chromosome size-uncorrelated homogenization of centromeric repetitive sequences in New World quails
Chromosome Research, 2014Co-Authors: Satoshi Ishishita, Yuri Tsuruta, Chizuko Nishida, Darren K Griffin, Masaoki Tsudzuki, Atsushi Nakamura, Yoichi MatsudaAbstract:Many families of centromeric repetitive DNA sequences isolated from Struthioniformes, Galliformes, Falconiformes, and Passeriformes are localized primarily to microchromosomes. However, it is unclear whether chromosome size-correlated homogenization is a common characteristic of centromeric repetitive sequences in Aves. New World and Old World quails have the typical avian karyotype comprising chromosomes of two distinct sizes, and C-positive heterochromatin is distributed in centromeric regions of most autosomes and the whole W chromosome. We isolated six types of centromeric repetitive sequences from three New World quail species ( Colinus virginianus , CVI; Callipepla californica , CCA; and Callipepla squamata , CSQ; Odontophoridae) and one Old World quail species ( Alectoris chukar , ACH; Phasianidae), and characterized the sequences by nucleotide sequencing, chromosome in situ hybridization, and filter hybridization. The 385-bp CVI- Msp I, 591-bp CCA- BamH I, 582-bp CSQ- BamH I, and 366-bp ACH- Sau 3AI fragments exhibited tandem arrays of the monomer unit, and the 224-bp CVI- Hae III and 135-bp CCA- Hae III fragments were composed of minisatellite-like and microsatellite-like repeats, respectively. ACH- Sau 3AI was a homolog of the chicken nuclear membrane repeat sequence, whose homologs are common in Phasianidae. CVI- Msp I, CCA- BamH I, and CSQ- BamH I showed high homology and were specific to the Odontophoridae. CVI- Msp I was localized to microchromosomes, whereas CVI- Hae III, CCA- BamH I, and CSQ- BamH I were mapped to almost all chromosomes. CCA- Hae III was localized to five pairs of macrochromosomes and most microchromosomes. ACH- Sau 3AI was distributed in three pairs of macrochromosomes and all microchromosomes. Centromeric repetitive sequences may be homogenized in chromosome size-correlated and -uncorrelated manners in New World quails, although there may be a mechanism that causes homogenization of centromeric repetitive sequences primarily between microchromosomes, which is commonly observed in phasianid birds.
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The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds
Chromosome Research, 2007Co-Authors: Chizuko Nishida-umehara, Yoichi Matsuda, Junko Ishijima, Yayoi Tsuda, Junko Ando, Atushi Fujiwara, Darren K GriffinAbstract:Palaeognathous birds (Struthioniformes and Tinamiformes) have morphologically conserved karyotypes and less differentiated ZW sex chromosomes. To delineate interspecific chromosome orthologies in palaeognathous birds we conducted comparative chromosome painting with chicken ( Gallus gallus , GGA) chromosome 1–9 and Z chromosome paints (GGA1–9 and GGAZ) for emu, double-wattled cassowary, ostrich, greater rhea, lesser rhea and elegant crested tinamou. All six species showed the same painting patterns: each probe was hybridized to a single pair of chromosomes with the exception that the GGA4 was hybridized to the fourth largest chromosome and a single pair of microchromosomes. The GGAZ was also hybridized to the entire region of the W chromosome, indicating that extensive homology remains between the Z and W chromosomes on the molecular level. Comparative FISH mapping of four Z- and/or W-linked markers, the ACO1/IREBP , ZOV3 and CHD1 genes and the EE0.6 sequence, revealed the presence of a small deletion in the proximal region of the long arm of the W chromosome in greater rhea and lesser rhea. These results suggest that the karyotypes and sex chromosomes of palaeognathous birds are highly conserved not only morphologically, but also at the molecular level; moreover, palaeognathous birds appear to retain the ancestral lineage of avian karyotypes.
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Comparison of the Z and W sex chromosomal architectures in elegant crested tinamou (Eudromia elegans) and ostrich (Struthio camelus) and the process of sex chromosome differentiation in palaeognathous birds
Chromosoma, 2007Co-Authors: Yayoi Tsuda, Chizuko Nishida-umehara, Kazuhiko Yamada, Junko Ishijima, Yoichi MatsudaAbstract:To clarify the process of avian sex chromosome differentiation in palaeognathous birds, we performed molecular and cytogenetic characterization of W chromosome-specific repetitive DNA sequences for elegant crested tinamou ( Eudromia elegans , Tinamiformes) and constructed comparative cytogenetic maps of the Z and W chromosomes with nine chicken Z-linked gene homologues for E. elegans and ostrich ( Struthio camelus , Struthioniformes). A novel family of W-specific repetitive sequences isolated from E. elegans was found to be composed of guanine- and cytosine-rich 293-bp elements that were tandemly arrayed in the genome as satellite DNA. No nucleotide sequence homologies were found for the Struthioniformes and neognathous birds. The comparative cytogenetic maps of the Z and W chromosomes of E. elegans and S. camelus revealed that there are partial deletions in the proximal regions of the W chromosomes in the two species, and the W chromosome is more differentiated in E. elegans than in S. camelus . These results suggest that a deletion firstly occurred in the proximal region close to the centromere of the acrocentric proto-W chromosome and advanced toward the distal region. In E. elegans , the W-specific repeated sequence elements were amplified site-specifically after deletion of a large part of the W chromosome occurred.
Francisco Carlos Rodrigues De ,oliveira - One of the best experts on this subject based on the ideXlab platform.
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hematological and morphometric differences of blood cells from rheas rhea americana Struthioniformes rheidae on two conservation farms
Brazilian Journal of Biology, 2017Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveiraAbstract:Atualmente exames de sangue sao ferramentas indispensaveis na medicina aviaria. Este estudo teve como objetivo descrever e comparar valores hematologicos e morfometricos de eritrocitos, leucocitos e trombocitos de emas, Rhea americana. Para isso, foram selecionadas 58 emas de ambos os sexos de dois criatorios, um em Cachoeiro do Itapemirim, Espirito Santo (ES) e o outro em Sao Carlos, Sao Paulo (SP). Amostras de sangue foram coletadas e realizadas a hematimetria, Hto e dosagem de Hb que foram utilizados nos calculos dos indices hematimetricos. Tambem foram efetuadas as contagens total e diferencial de leucocitos, alem da dosagem de proteinas total e do fibrinogenio sericos. Os resultados referentes as duas propriedades analisadas foram comparados pelo Teste t. Ocorreram diferencas nos parâmetros da serie vermelha entre as aves dos dois criatorios devido a um processo de anemia macrocitica hipocromica observado nas emas do ES. Os valores para a serie vermelha e indices hematimetricos como: hematimetria, 2,81±0,15 x106/μl; Hto, 44,20±2,86%; Hb, 12,12±0,74 g/dL; VCM, 15,75±0,89 fL; HCM, 43,18±1,82 pg; CHCM, 27,44±0,80, alem dos valores da serie branca como: leucometria total, 12.072±4.116/μL, heterofilo, 64,10±9,90%, eosinofilo, 2,05±2,06%, monocito, 6,40±2,99%, linfocito, 26,93±9,62%, basofilo, 0,52±1,27% foram sugeridos como os de referencia para as emas criadas no Brasil. Diferencas estatisticas foram observadas no comprimento de eritrocitos, o qual estava maior nas emas de SP do que aquelas do ES. Em relacao a largura, os heterofilos, linfocitos e monocitos das aves do ES foram estatisticamente maior do que aquelas das aves de SP. Concluiu-se que emas podem apresentar alteracoes morfometricas de celulas sanguineas e diferencas nos elementos do sangue de acordo com o tipo de manejo a que sao submetidos.
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hematological and morphometric differences of blood cells from rheas rhea americana Struthioniformes rheidae on two conservation farms
Brazilian Journal of Biology, 2017Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveiraAbstract:Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espirito Santo (ES), and the other in Sao Carlos, Sao Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.
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Diferenças hematológicas e morfométricas de células sanguineas de emas, Rhea americana (Struthioniformes: Rheidae) em dois criatórios conservacionistas
'FapUNIFESP (SciELO)', 2017Co-Authors: Gallo, Samira Salim Mello, Ederli, Nicole Brand, Francisco Carlos Rodrigues De ,oliveiraAbstract:Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espírito Santo (ES), and the other in São Carlos, São Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.Atualmente exames de sangue são ferramentas indispensáveis na medicina aviária. Este estudo teve como objetivo descrever e comparar valores hematológicos e morfométricos de eritrócitos, leucócitos e trombócitos de emas, Rhea americana. Para isso, foram selecionadas 58 emas de ambos os sexos de dois criatórios, um em Cachoeiro do Itapemirim, Espírito Santo (ES) e o outro em São Carlos, São Paulo (SP). Amostras de sangue foram coletadas e realizadas a hematimetria, Hto e dosagem de Hb que foram utilizados nos cálculos dos índices hematimétricos. Também foram efetuadas as contagens total e diferencial de leucócitos, além da dosagem de proteínas total e do fibrinogênio séricos. Os resultados referentes às duas propriedades analisadas foram comparados pelo Teste t. Ocorreram diferenças nos parâmetros da série vermelha entre as aves dos dois criatórios devido a um processo de anemia macrocítica hipocrômica observado nas emas do ES. Os valores para a série vermelha e índices hematimétricos como: hematimetria, 2,81±0,15 x106/μl; Hto, 44,20±2,86%; Hb, 12,12±0,74 g/dL; VCM, 15,75±0,89 fL; HCM, 43,18±1,82 pg; CHCM, 27,44±0,80, além dos valores da série branca como: leucometria total, 12.072±4.116/μL, heterofilo, 64,10±9,90%, eosinófilo, 2,05±2,06%, monócito, 6,40±2,99%, linfócito, 26,93±9,62%, basófilo, 0,52±1,27% foram sugeridos como os de referência para as emas criadas no Brasil. Diferenças estatísticas foram observadas no comprimento de eritrócitos, o qual estava maior nas emas de SP do que aquelas do ES. Em relação à largura, os heterófilos, linfócitos e monócitos das aves do ES foram estatisticamente maior do que aquelas das aves de SP. Concluiu-se que emas podem apresentar alterações morfométricas de células sanguíneas e diferenças nos elementos do sangue de acordo com o tipo de manejo a que são submetidos
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Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds
Brazilian journal of biology = Revista brasleira de biologia, 2015Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Murilo De Oliveira Bôa-morte, Francisco Carlos Rodrigues De ,oliveiraAbstract:Exames de sangue sao uma ferramenta indispensavel na medicina de aves. Este estudo teve como objetivo descrever valores e aspectos da hematologia de emas, Rhea americana, bem como analisar a morfologia e morfometria de todas as celulas sanguineas. Cinquenta e oito emas adultas de ambos os sexos de dois criatorios, um em Cachoeiro do Itapemirim, ES e o outro em Sao Carlos, SP foram selecionadas. Amostras de sangue foram coletadas e CTH, Ht e niveis de Hb foram mensurados e usados para o calculo dos indices hematimetricos. A contagem total e diferencial de leucocitos, assim como, PPT e fibrinogenio foram determinados. Eritrocitos, leucocitos e trombocitos foram identificados e caracterizados morfologicamente. Os valores para serie vermelha e indices hematimetricos foram: CTH (2,81±0,15×106/μL), Ht (44,20±2,86%), Hb (12,12±0,74 g/dL), VCM (15,75±0,89 fL), HCM (43,18±1,82 pg), CHCM (27,44±0,80 g/dL); os valores da serie branca foram: CTL (12,072±4116/μL), heterofilos (64,10±9,90%), eosinofilos (2,05±2,06%), monocitos (6,40±2,99%), linfocitos (26,93±9,62%), basofilos (0,52±1,27%). Pode-se concluir que, na media, celulas sanguineas de emas sao maiores que as de outras aves, porem, nao e possivel diferenciar estas celulas em esfregacos somente pelo seu tamanho. Alem disso, leucocitos de emas possuem diferentes componentes e coloracao como acontece em outras especies de aves, no entanto, nao ha nenhum componente ou aspectos tintoriais exclusivo para a especie.
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Características hematológicas, morfológicas e morfométricas de células sanguíneas de emas, Rhea americana (Struthioniformes: Rheidae): um padrão para aves brasileiras
'FapUNIFESP (SciELO)', 2015Co-Authors: Gallo, Samira Salim Mello, Ederli, Nicole Brand, BÔa-morte, Murilo De Oliveira, Francisco Carlos Rodrigues De ,oliveiraAbstract:Blood exams are an indispensable tool in bird medicine. This study aimed at describing values and aspects of rheas' hematology, Rhea americana, as well as analyzing the morphology and morphometry of all blood cells. Fifty eight adult rheas of both sexes from two farms, one in Cachoeiro de Itapemirim, Espírito Santo State and the other in São Carlos, São Paulo State, were selected. Blood samples were taken and RBC count, PCV and Hb levels measured and used in hematimetric indexes calculations. The total and differentiated leukocyte counts, as well as the TPP and fibrinogen were determined. Erythrocytes, leukocytes and thrombocytes were identified and characterized morphologically. The values for the red series and hematimetric indexes were: RBC (2.81±0.15×106/μL), PCV (44.20±2.86%), Hb (12.12±0.74 g/dL), MCV (15.75±0.89 fL), MCH (43.18±1.82 pg), MCHC (27.44±0.80 g/dL); the values of white series were: WBC (12.072±4116/μL), heterophils (64.10±9.90%), eosinophils (2.05±2.06%), monocytes (6.40±2.99%), lymphocytes (26.93±9.62%), basophils (0.52±1.27%). One may conclude that on average, rheas' blood cells are larger than those of other birds, but these cells in smears cannot be differentiated only by their size. Besides rheas' leukocytes have different components and coloring as in other bird species, however, there are no components or staining aspects unique to the species.Exames de sangue são uma ferramenta indispensável na medicina de aves. Este estudo teve como objetivo descrever valores e aspectos da hematologia de emas, Rhea americana, bem como analisar a morfologia e morfometria de todas as células sanguíneas. Cinquenta e oito emas adultas de ambos os sexos de dois criatórios, um em Cachoeiro do Itapemirim, ES e o outro em São Carlos, SP foram selecionadas. Amostras de sangue foram coletadas e CTH, Ht e níveis de Hb foram mensurados e usados para o cálculo dos índices hematimétricos. A contagem total e diferencial de leucócitos, assim como, PPT e fibrinogênio foram determinados. Eritrócitos, leucócitos e trombócitos foram identificados e caracterizados morfologicamente. Os valores para série vermelha e índices hematimétricos foram: CTH (2,81±0,15×106/μL), Ht (44,20±2,86%), Hb (12,12±0,74 g/dL), VCM (15,75±0,89 fL), HCM (43,18±1,82 pg), CHCM (27,44±0,80 g/dL); os valores da série branca foram: CTL (12,072±4116/μL), heterófilos (64,10±9,90%), eosinófilos (2,05±2,06%), monócitos (6,40±2,99%), linfócitos (26,93±9,62%), basófilos (0,52±1,27%). Pode-se concluir que, na média, células sanguíneas de emas são maiores que as de outras aves, porém, não é possível diferenciar estas células em esfregaços somente pelo seu tamanho. Além disso, leucócitos de emas possuem diferentes componentes e coloração como acontece em outras espécies de aves, no entanto, não há nenhum componente ou aspectos tintoriais exclusivo para a espécie
Nicole Brand Ederli - One of the best experts on this subject based on the ideXlab platform.
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hematological and morphometric differences of blood cells from rheas rhea americana Struthioniformes rheidae on two conservation farms
Brazilian Journal of Biology, 2017Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveiraAbstract:Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espirito Santo (ES), and the other in Sao Carlos, Sao Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.
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hematological and morphometric differences of blood cells from rheas rhea americana Struthioniformes rheidae on two conservation farms
Brazilian Journal of Biology, 2017Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveiraAbstract:Atualmente exames de sangue sao ferramentas indispensaveis na medicina aviaria. Este estudo teve como objetivo descrever e comparar valores hematologicos e morfometricos de eritrocitos, leucocitos e trombocitos de emas, Rhea americana. Para isso, foram selecionadas 58 emas de ambos os sexos de dois criatorios, um em Cachoeiro do Itapemirim, Espirito Santo (ES) e o outro em Sao Carlos, Sao Paulo (SP). Amostras de sangue foram coletadas e realizadas a hematimetria, Hto e dosagem de Hb que foram utilizados nos calculos dos indices hematimetricos. Tambem foram efetuadas as contagens total e diferencial de leucocitos, alem da dosagem de proteinas total e do fibrinogenio sericos. Os resultados referentes as duas propriedades analisadas foram comparados pelo Teste t. Ocorreram diferencas nos parâmetros da serie vermelha entre as aves dos dois criatorios devido a um processo de anemia macrocitica hipocromica observado nas emas do ES. Os valores para a serie vermelha e indices hematimetricos como: hematimetria, 2,81±0,15 x106/μl; Hto, 44,20±2,86%; Hb, 12,12±0,74 g/dL; VCM, 15,75±0,89 fL; HCM, 43,18±1,82 pg; CHCM, 27,44±0,80, alem dos valores da serie branca como: leucometria total, 12.072±4.116/μL, heterofilo, 64,10±9,90%, eosinofilo, 2,05±2,06%, monocito, 6,40±2,99%, linfocito, 26,93±9,62%, basofilo, 0,52±1,27% foram sugeridos como os de referencia para as emas criadas no Brasil. Diferencas estatisticas foram observadas no comprimento de eritrocitos, o qual estava maior nas emas de SP do que aquelas do ES. Em relacao a largura, os heterofilos, linfocitos e monocitos das aves do ES foram estatisticamente maior do que aquelas das aves de SP. Concluiu-se que emas podem apresentar alteracoes morfometricas de celulas sanguineas e diferencas nos elementos do sangue de acordo com o tipo de manejo a que sao submetidos.
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Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds
Brazilian journal of biology = Revista brasleira de biologia, 2015Co-Authors: Samira Salim Mello Gallo, Nicole Brand Ederli, Murilo De Oliveira Bôa-morte, Francisco Carlos Rodrigues De ,oliveiraAbstract:Exames de sangue sao uma ferramenta indispensavel na medicina de aves. Este estudo teve como objetivo descrever valores e aspectos da hematologia de emas, Rhea americana, bem como analisar a morfologia e morfometria de todas as celulas sanguineas. Cinquenta e oito emas adultas de ambos os sexos de dois criatorios, um em Cachoeiro do Itapemirim, ES e o outro em Sao Carlos, SP foram selecionadas. Amostras de sangue foram coletadas e CTH, Ht e niveis de Hb foram mensurados e usados para o calculo dos indices hematimetricos. A contagem total e diferencial de leucocitos, assim como, PPT e fibrinogenio foram determinados. Eritrocitos, leucocitos e trombocitos foram identificados e caracterizados morfologicamente. Os valores para serie vermelha e indices hematimetricos foram: CTH (2,81±0,15×106/μL), Ht (44,20±2,86%), Hb (12,12±0,74 g/dL), VCM (15,75±0,89 fL), HCM (43,18±1,82 pg), CHCM (27,44±0,80 g/dL); os valores da serie branca foram: CTL (12,072±4116/μL), heterofilos (64,10±9,90%), eosinofilos (2,05±2,06%), monocitos (6,40±2,99%), linfocitos (26,93±9,62%), basofilos (0,52±1,27%). Pode-se concluir que, na media, celulas sanguineas de emas sao maiores que as de outras aves, porem, nao e possivel diferenciar estas celulas em esfregacos somente pelo seu tamanho. Alem disso, leucocitos de emas possuem diferentes componentes e coloracao como acontece em outras especies de aves, no entanto, nao ha nenhum componente ou aspectos tintoriais exclusivo para a especie.
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further study of codiostomum struthionis horst 1885 railliet and henry 1911 nematoda strongylidae parasite of ostriches struthio camelus linnaeus 1758 aves Struthioniformes
Veterinary Parasitology, 2008Co-Authors: Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveira, Maria De Lurdes De Azevedo RodriguesAbstract:Abstract Codiostomum struthionis is a nematode parasite of the ostrich caecum. Little is known about its pathology, being considered by many authors as a non-pathogenic parasite. Infections by C. struthionis are sometimes overlooked because its eggs are indistinguishable from another ostrich nematode, Libyostrongylus spp. Fecal cultures and infective larvae identification are necessary for proper identification. The aim of this study is to provide improved morphological characterization of adults and infective larvae of C. struthionis. Ten caeca of adult ostriches were collected and washed in 0.09% saline solution. Male and female nematodes were collected and quantified separately. Nematodes were fixed in A.F.A. for optical microscopy or fixed in Karnovsky solution for scanning electron microscopy. To obtain infective larvae, fecal samples were collected at sites of high concentration of parasites in the caeca and fecal cultured. The resultant larvae were identified and measured with light microscope at 400×. Nine of the 10 slaughtered ostriches were parasitized by C. struthionis. All nematodes were found in the distal third of the caeca. A total of 566 parasites were recovered (234 males and 332 females). All the cultured larvae had characteristics of C. struthionis (rounded cephalic region with a flat extremity, an acute larvae tail termination and a long and filamentous sheath tail). All the adult parasites were characterized as C. struthionis. Through the analysis of the infective larvae it was determined that the morphology of the larvae tail was the best trait to use in the distinction of this species (live bird diagnosis).
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mixed infection by libyostrongylus douglassii and l dentatus nematoda trichostrongylidae in struthio camelus ratites Struthioniformes from brazil with further morphological characterization of adults
Veterinary Parasitology, 2008Co-Authors: Nicole Brand Ederli, Sergio Fernandes Bonadiman, Antonio Henrique Almeida De Moraes Neto, Renato Augusto Damatta, Clovis De Paula SantosAbstract:The genus Libyostrongylus includes three species, L. douglassii, L. dentatus and L. magnus that occur as parasites in the proventriculus of Struthio camelus. We confirmed a mixed infection by L. douglassii and L. dentatus in farmed ostriches from the southeast of Brazil for the first time, and provided new information on some morphological characters that differentiate these species. Adult nematodes collected from the proventriculus of ostriches were observed by light and scanning electron microscopy. Morphologic characterization and morphometric analysis of the nematodes enabled the distinction of both species and corroborated results of prior studies. Specimens of L. dentatus have a buccal capsule with a prominent esophageal tooth. Furthermore, males and females of L. dentatus were larger (4954 and 9347 microm) than those of L. douglassii (3411 and 4229 microm), but measurements for most characters in both species were smaller then those previously reported. Besides, the cephalic structure based on scanning electron microscopy differs, and L. dentatus has thick lips with round papillae, whereas L. douglassii has fine lips with lengthened papillae. The confirmation of both species in South America strongly suggests that the mixed infection may be common in farmed ostriches.
Maria De Lurdes De Azevedo Rodrigues - One of the best experts on this subject based on the ideXlab platform.
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further study of codiostomum struthionis horst 1885 railliet and henry 1911 nematoda strongylidae parasite of ostriches struthio camelus linnaeus 1758 aves Struthioniformes
Veterinary Parasitology, 2008Co-Authors: Nicole Brand Ederli, Francisco Carlos Rodrigues De ,oliveira, Maria De Lurdes De Azevedo RodriguesAbstract:Abstract Codiostomum struthionis is a nematode parasite of the ostrich caecum. Little is known about its pathology, being considered by many authors as a non-pathogenic parasite. Infections by C. struthionis are sometimes overlooked because its eggs are indistinguishable from another ostrich nematode, Libyostrongylus spp. Fecal cultures and infective larvae identification are necessary for proper identification. The aim of this study is to provide improved morphological characterization of adults and infective larvae of C. struthionis. Ten caeca of adult ostriches were collected and washed in 0.09% saline solution. Male and female nematodes were collected and quantified separately. Nematodes were fixed in A.F.A. for optical microscopy or fixed in Karnovsky solution for scanning electron microscopy. To obtain infective larvae, fecal samples were collected at sites of high concentration of parasites in the caeca and fecal cultured. The resultant larvae were identified and measured with light microscope at 400×. Nine of the 10 slaughtered ostriches were parasitized by C. struthionis. All nematodes were found in the distal third of the caeca. A total of 566 parasites were recovered (234 males and 332 females). All the cultured larvae had characteristics of C. struthionis (rounded cephalic region with a flat extremity, an acute larvae tail termination and a long and filamentous sheath tail). All the adult parasites were characterized as C. struthionis. Through the analysis of the infective larvae it was determined that the morphology of the larvae tail was the best trait to use in the distinction of this species (live bird diagnosis).
Martin Kváč - One of the best experts on this subject based on the ideXlab platform.
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more than a rabbit s tale encephalitozoon spp in wild mammals and birds
International journal for parasitology. Parasites and wildlife, 2016Co-Authors: Barbara Hinney, Bohumil Sak, Anja Joachim, Martin KváčAbstract:Within the microsporidian genus Encephalitozoon, three species, Encephalitozoon cuniculi, Encephalitozoon hellem and Encephalitozoon intestinalis have been described. Several orders of the Class Aves (Passeriformes, Psittaciformes, Apodiformes, Ciconiiformis, Gruiformes, Columbiformes, Suliformes, Podicipediformes, Anseriformes, Struthioniformes, Falconiformes) and of the Class Mammalia (Rodentia, Lagomorpha, Primates, Artyodactyla, Soricomorpha, Chiroptera, Carnivora) can become infected. Especially E. cuniculi has a very broad host range while E. hellem is mainly distributed amongst birds. E. intestinalis has so far been detected only sporadically in wild animals. Although genotyping allows the identification of strains with a certain host preference, recent studies have demonstrated that they have no strict host specificity. Accordingly, humans can become infected with any of the four strains of E. cuniculi as well as with E. hellem or E. intestinalis, the latter being the most common. Especially, but not exclusively, immunocompromised people are at risk. Environmental contamination with as well as direct transmission of Encephalitozoon is therefore highly relevant for public health. Moreover, endangered species might be threatened by the spread of pathogens into their habitats. In captivity, clinically overt and often fatal disease seems to occur frequently. In conclusion, Encephalitozoon appears to be common in wild warm-blooded animals and these hosts may present important reservoirs for environmental contamination and maintenance of the pathogens. Similar to domestic animals, asymptomatic infections seem to occur frequently but in captive wild animals severe disease has also been reported. Detailed investigations into the epidemiology and clinical relevance of these microsporidia will permit a full appraisal of their role as pathogens.
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More than a rabbit's tale – Encephalitozoon spp. in wild mammals and birds
International journal for parasitology. Parasites and wildlife, 2016Co-Authors: Barbara Hinney, Bohumil Sak, Anja Joachim, Martin KváčAbstract:Within the microsporidian genus Encephalitozoon, three species, Encephalitozoon cuniculi, Encephalitozoon hellem and Encephalitozoon intestinalis have been described. Several orders of the Class Aves (Passeriformes, Psittaciformes, Apodiformes, Ciconiiformis, Gruiformes, Columbiformes, Suliformes, Podicipediformes, Anseriformes, Struthioniformes, Falconiformes) and of the Class Mammalia (Rodentia, Lagomorpha, Primates, Artyodactyla, Soricomorpha, Chiroptera, Carnivora) can become infected. Especially E. cuniculi has a very broad host range while E. hellem is mainly distributed amongst birds. E. intestinalis has so far been detected only sporadically in wild animals. Although genotyping allows the identification of strains with a certain host preference, recent studies have demonstrated that they have no strict host specificity. Accordingly, humans can become infected with any of the four strains of E. cuniculi as well as with E. hellem or E. intestinalis, the latter being the most common. Especially, but not exclusively, immunocompromised people are at risk. Environmental contamination with as well as direct transmission of Encephalitozoon is therefore highly relevant for public health. Moreover, endangered species might be threatened by the spread of pathogens into their habitats. In captivity, clinically overt and often fatal disease seems to occur frequently. In conclusion, Encephalitozoon appears to be common in wild warm-blooded animals and these hosts may present important reservoirs for environmental contamination and maintenance of the pathogens. Similar to domestic animals, asymptomatic infections seem to occur frequently but in captive wild animals severe disease has also been reported. Detailed investigations into the epidemiology and clinical relevance of these microsporidia will permit a full appraisal of their role as pathogens.
