The Experts below are selected from a list of 29061 Experts worldwide ranked by ideXlab platform
Tony Jun Huang - One of the best experts on this subject based on the ideXlab platform.
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three dimensional numerical simulation and experimental investigation of boundary driven streaming in Surface Acoustic Wave microfluidics
Lab on a Chip, 2018Co-Authors: Chuyi Chen, Francesco Costanzo, Nitesh Nama, Steven Peiran Zhang, Zhangming Mao, Pohsun Huang, Yun Jing, Xiasheng Guo, Tony Jun HuangAbstract:Acoustic streaming has been widely used in microfluidics to manipulate various micro−/nano-objects. In this work, Acoustic streaming activated by interdigital transducers (IDT) immersed in highly viscous oil is studied numerically and experimentally. In particular, we developed a modeling strategy termed the “slip velocity method” that enables a 3D simulation of Surface Acoustic Wave microfluidics in a large domain (4 × 4 × 2 mm3) and at a high frequency (23.9 MHz). The experimental and numerical results both show that on top of the oil, all the Acoustic streamlines converge at two horizontal stagnation points above the two symmetric sides of the IDT. At these two stagnation points, water droplets floating on the oil can be trapped. Based on these characteristics of the Acoustic streaming field, we designed a Surface Acoustic Wave microfluidic device with an integrated IDT array fabricated on a 128° YX LiNbO3 substrate to perform programmable, contactless droplet manipulation. By activating IDTs accordingly, the water droplets on the oil can be moved to the corresponding traps. With its excellent capability for manipulating droplets in a highly programmable, controllable manner, our Surface Acoustic Wave microfluidic devices are valuable for on-chip contactless sample handling and chemical reactions.
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standing Surface Acoustic Wave ssaw based cell washing
Lab on a Chip, 2015Co-Authors: Sixing Li, Craig E Cameron, Nitesh Nama, Xiaoyun Ding, Peng Li, Yuchao Chen, Lin Wang, Tony Jun HuangAbstract:Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing Surface Acoustic Wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the Acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing Surface Acoustic Wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.
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standing Surface Acoustic Wave based cell coculture
Analytical Chemistry, 2014Co-Authors: Sixing Li, Craig E Cameron, Xiaoyun Ding, Peng Li, Yuchao Chen, Tony Jun HuangAbstract:Precise reconstruction of heterotypic cell–cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing Surface Acoustic Wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell–cell interactions.
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standing Surface Acoustic Wave ssaw based microfluidic cytometer
Lab on a Chip, 2014Co-Authors: Yuchao Chen, Lin Wang, Pohsun Huang, Ahmad Ahsan Nawaz, Yanhui Zhao, Phillip J Mccoy, Stewart J Levine, Tony Jun HuangAbstract:The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing Surface Acoustic Wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of ~1000 events s−1 when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power Surface Acoustic Waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.
Camelia Bala - One of the best experts on this subject based on the ideXlab platform.
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Enhanced sensitive love Wave Surface Acoustic Wave sensor designed for immunoassay formats
Sensors (Switzerland), 2015Co-Authors: Mihaela Puiu, Cristian Viespe, Lucian Rotariu, Simona Brajnicov, Ana-maria Gurban, Camelia BalaAbstract:We report a Love Wave Surface Acoustic Wave (LW-SAW) immunosensor designed for the detection of high molecular weight targets in liquid samples, amenable also for low molecular targets in Surface competition assays. We implemented a label-free interaction protocol similar to other Surface plasmon resonance bioassays having the advantage of requiring reduced time analysis. The fabricated LW-SAW sensor supports the detection of the target in the nanomolar range, and can be ultimately incorporated in portable devices, suitable for point-of-care testing (POCT) applications.
A.j. Walton - One of the best experts on this subject based on the ideXlab platform.
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Surface Acoustic Wave nebulization on nanocrystalline zno film
Applied Physics Letters, 2012Co-Authors: Yong Qing Fu, Yifan Li, Frank Placido, A.j. WaltonAbstract:Surface Acoustic Wave(SAW) nebulization/atomization has been realised on thin ZnOfilm based SAWdevices. The Surface Acoustic Wave nebulization (SAWN) process has been observed to produce significant mist generation and ejected satellitedroplets. By modifying the geometry of the interdigitated transducers to reduce the Wavelengths from 400 μm to 120 μm, higher frequency SAWN has been achieved by increasing radio frequency driving frequencies from 11.8 MHz to 37.2 MHz, respectively. Compared with the commonly used LiNbO3 SAWN devices,ZnOfilmdevices exhibit better thermal dissipation, and to date, they have shown no susceptibility to substrate failure during fabrication or operation. They also have the added advantage of the technology being suitable for direct integration with microsystems and integrated circuit microelectronics.
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integrated microfluidics system using Surface Acoustic Wave and electrowetting on dielectrics technology
Biomicrofluidics, 2012Co-Authors: Stuart Brodie, M Alghane, A.j. WaltonAbstract:This paper presents integrated microfluidic lab-on-a-chip technology combining Surface Acoustic Wave (SAW) and electro-wetting on dielectric (EWOD). This combination has been designed to provide enhanced microfluidic functionality and the integrated devices have been fabricated using a single mask lithographic process. The integrated technology uses EWOD to guide and precisely position microdroplets which can then be actuated by SAW devices for particle concentration, Acoustic streaming, mixing and ejection, as well as for sensing using a shear-horizontal Wave SAW device. A SAW induced force has also been employed to enhance the EWOD droplet splitting function.
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streaming phenomena in microdroplets induced by rayleigh Surface Acoustic Wave
Journal of Applied Physics, 2011Co-Authors: M Alghane, Yifan Li, Yong Qing Fu, Baixin Chen, M P Y Desmulliez, A.j. WaltonAbstract:This paper reports the numerical simulation and experimental characterization of three-dimensional Acoustic streaming behavior of a liquiddroplet subjected to a Rayleigh Surface Acoustic Wave. The streaming behavior of the droplet was studied as a function of radio-frequency (RF) power, aperture of the interdigitated transducer, and size of the liquiddroplet. The hydrodynamic flow field within the droplet was determined by solving the laminar incompressible Navier–Stoke’s equations. The numerical and experimental results are shown to be in good agreement over the range of parameters examined. The ratios of the position of butterfly central line (axis of rotation) to radius of the droplet are demonstrated to be fairly constant for moderate droplet volumes and to vary by less than 12% at large droplet volumes. Besides that, an increase in the RF power and a decrease in the droplet size result in an increased Surface Acoustic Wave(SAW) streaming velocity. The numerical results also suggest that a maximum streaming velocity is achieved when the SAW width is approximately half of the droplet radius.
Yuchao Chen - One of the best experts on this subject based on the ideXlab platform.
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standing Surface Acoustic Wave ssaw based cell washing
Lab on a Chip, 2015Co-Authors: Sixing Li, Craig E Cameron, Nitesh Nama, Xiaoyun Ding, Peng Li, Yuchao Chen, Lin Wang, Tony Jun HuangAbstract:Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing Surface Acoustic Wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the Acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing Surface Acoustic Wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.
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standing Surface Acoustic Wave based cell coculture
Analytical Chemistry, 2014Co-Authors: Sixing Li, Craig E Cameron, Xiaoyun Ding, Peng Li, Yuchao Chen, Tony Jun HuangAbstract:Precise reconstruction of heterotypic cell–cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing Surface Acoustic Wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell–cell interactions.
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standing Surface Acoustic Wave ssaw based microfluidic cytometer
Lab on a Chip, 2014Co-Authors: Yuchao Chen, Lin Wang, Pohsun Huang, Ahmad Ahsan Nawaz, Yanhui Zhao, Phillip J Mccoy, Stewart J Levine, Tony Jun HuangAbstract:The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing Surface Acoustic Wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of ~1000 events s−1 when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power Surface Acoustic Waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.
Wojtek Wlodarski - One of the best experts on this subject based on the ideXlab platform.
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graphene like nano sheets for Surface Acoustic Wave gas sensor applications
Chemical Physics Letters, 2009Co-Authors: R. Arsat, P. G. Spizziri, Scott Gilje, Richard Barry Kaner, Michael Breedon, Kourosh Kalantarzadeh, Mahnaz Shafiei, Wojtek WlodarskiAbstract:Abstract The gas sensing properties of graphene-like nano-sheets deposited on 36° YX lithium tantalate (LiTaO 3 ) Surface Acoustic Wave (SAW) transducers are reported. The thin graphene-like nano-sheets were produced via the reduction of graphite oxide which was deposited on SAW interdigitated transducers (IDTs). Their sensing performance was assessed towards hydrogen (H 2 ) and carbon monoxide (CO) in a synthetic air carrier gas at room temperature (25 °C) and 40 °C. Raman and X-ray photoelectron spectroscopy (XPS) revealed that the deposited graphite oxide (GO) was not completely reduced creating small, graphitic nanocrystals ∼2.7 nm in size.
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Graphene-like nano-sheets for Surface Acoustic Wave gas sensor applications
Chemical Physics Letters, 2009Co-Authors: R. Arsat, P. G. Spizziri, Scott Gilje, Mojtaba Shafiei, Richard Barry Kaner, Michael Breedon, Kourosh Kalantar-zadeh, Wojtek WlodarskiAbstract:The gas sensing properties of graphene-like nano-sheets deposited on 36° YX lithium tantalate (LiTaO3) Surface Acoustic Wave (SAW) transducers are reported. The thin graphene-like nano-sheets were produced via the reduction of graphite oxide which was deposited on SAW interdigitated transducers (IDTs). Their sensing performance was assessed towards hydrogen (H2) and carbon monoxide (CO) in a synthetic air carrier gas at room temperature (25 °C) and 40 °C. Raman and X-ray photoelectron spectroscopy (XPS) revealed that the deposited graphite oxide (GO) was not completely reduced creating small, graphitic nanocrystals ∼2.7 nm in size. © 2008 Elsevier B.V.
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novel love mode Surface Acoustic Wave based immunosensors
Sensors and Actuators B-chemical, 2003Co-Authors: Kourosh Kalantarzadeh, Wojtek Wlodarski, Yuen Y Chen, K GalatsisAbstract:Love mode Surface Acoustic Wave (SAW) immunosensors were developed. The Love mode guiding layers in these sensors are ZnO and SiO2 thin films. It is shown that mass sensitivity of the devices with ZnO layer are larger than that of with SiO2 guiding layers. A system comprising dual delay line, based on ZnO/90° rotated ST-cut quartz crystal devices, was set up for conducting the immunosensing experiments. In these experiments adsorption of rat immunoglobulin G onto the active area of the sensor was monitored. Upon exposure to solutions containing IgG, the operational frequency of the system changed due to the interaction of IgG and a gold Surface on the active area of the sensor. Mass sensitivities were measured and frequency responses to various IgG concentrations are presented.
