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Mustafa Karatepe - One of the best experts on this subject based on the ideXlab platform.
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The Effect of the Serum Amino Acid Levels Thiosemicarbazone Derivatives and its Metal Complexes on Rats
Society of TURAZ AKADEMI, 2013Co-Authors: Mustafa Karatepe, Dilara KamanAbstract:Advers biological activities of Thiosemicarbazone (TSC) and Schiff base (SB) Derivatives have been widely studied in rats and in other animal species using different doses, times and routes of administration. To date, no attempt has been made to study alterations occurring in the amino acid profile in the effects of the Thiosemicarbazone Derivative and its metal complexes on the rats. At this study, the rats were injected subcutaneously with a new Thiosemicarbazone and its LH-Zn and LH-Cu complexes (25 mg/kg body weight) and then sacrified after 15th days. The aim of this study was to determine the effect of new compounds on the serum amino acid levels in rats. In the comparision done among groups, it was observed that serum amino acid concentrations were statistically changed (p < 0.05). [Med-Science 2013; 2(2.000): 591-600
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The Effect of the Serum Amino Acid Levels Thiosemicarbazone Derivatives and its Metal Complexes on Rats
Medicine Science | International Medical Journal, 2013Co-Authors: Mustafa Karatepe, Dilara KamanAbstract:Advers biological activities of Thiosemicarbazone (TSC) and Schiff base (SB) Derivatives have been widely studied in rats and in other animal species using different doses, times and routes of administration. To date, no attempt has been made to study alterations occurring in the amino acid profile in the effects of the Thiosemicarbazone Derivative and its metal complexes on the rats. At this study, the rats were injected subcutaneously with a new Thiosemicarbazone and its LH-Zn and LH-Cu complexes (25 mg/kg body weight) and then sacrified after 15th days. The aim of this study was to determine the effect of new compounds on the serum amino acid levels in rats. In the comparision done among groups, it was observed that serum amino acid concentrations were statistically changed (p < 0.05).
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antioxidant pro oxidant effect of the Thiosemicarbazone Derivative schiff base 4 1 phenylmethylcyclobutane 3 yl 2 2 hydroxybenzylidenehydrazino thiazole and its metal complexes on rats
Cell Biochemistry and Function, 2006Co-Authors: Mustafa Karatepe, Fikret KaratasAbstract:Adverse biological activities of Thiosemicarbazone (TSC) and Schiff base (SB) Derivatives have been widely studied in rats and in other animal species using different doses, times and routes of administration. However, there are few studies describing changes in some biochemical parameters in vivo which are indicative of oxidative stress in biological systems and of morphological changes of tissues. In this study, the rats were injected subcutaneously with a new Thiosemicarbazone thiazole ring containing a Schiff base (LH) and its Cu(L)2 and Zn(L)2 complexes (25 mg kg−1 body weight) and then sacrificed after 1, 2, 4, 8, 16, 32 and 64 days. The aim of this study was to determine the effect of the new compounds on the serum antioxidant vitamins (A, E, C), selenium (Se), malondialdehyde (MDA) levels, erythrocyte GSH-Px enzyme activity and morphological changes in the liver, kidney and adrenal gland tissues. It was observed that erythrocyte GSH-Px activity, serum MDA and vitamins A, E concentrations were statistically changed (p < 0.02), but serum levels of selenium, and vitamin C were not changed. In conclusion, the parameters measured show that Cu(L)2 caused considerable oxidative stress and Zn(L)2 behaved as an antioxidant. No oxidative stress in LH was observed compared to the control group. Copyright © 2005 John Wiley & Sons, Ltd.
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effects on rat testes of the Thiosemicarbazone Derivative schiff base 4 1 phenylmethylcyclobutane 3 yl 2 2 hydroxybenzylidenehydrazino thiazole and its cadmium ii complex
Cell Biochemistry and Function, 2005Co-Authors: Hakan Oner, Mustafa Karatepe, Fikret Karatas, Jale Oner, Ibrahim Yilmaz, Alaaddin CukurovaliAbstract:The aim of this study was to investigate structural and biochemical changes in testes of rats treated with the Thiosemicarbazone Derivative thiazole ring Schiff base, (4-(1-phenyl-methylcyclobutane-3-yl)-2-(2-hydroxybenzylidene-hydrazino) thiazole (L), and its Cd(II) complex (CdL2). The animals were divided into three groups. Group I was designated as control. The rats in groups II and III were injected subcutaneously with L or CdL2 respectively at 150-mg kg−1 doses at 3-day intervals for 15 days. At the end of the study, blood samples were collected for biochemical analysis, and testes were removed for histological examinations. Serum levels of vitamin A, E and MDA of the L-injected group were similar to the control group. While CdL2 treatment decreased serum vitamin A and E levels, it increased the MDA level compared to other groups. Histologically, the testes structures of L-treated animals were similar to the control. Spermatogenic cells in seminiferous tubules of CdL2-treated animals displayed necrosis. Nuclei of spermatogonia and primary spermatocytes were pyknotic and heterochromatic. Homogenous pink particles were present in place of the spermatids. The interstitial areas were oedematous and intertubular vessels were plugged. In conclusion, the present results indicate that L does not cause biochemical and morphological alterations, but its Cd(II) complex has degenerative effects in normal rat testes. Copyright © 2004 John Wiley & Sons, Ltd.
Fikret Karatas - One of the best experts on this subject based on the ideXlab platform.
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antioxidant pro oxidant effect of the Thiosemicarbazone Derivative schiff base 4 1 phenylmethylcyclobutane 3 yl 2 2 hydroxybenzylidenehydrazino thiazole and its metal complexes on rats
Cell Biochemistry and Function, 2006Co-Authors: Mustafa Karatepe, Fikret KaratasAbstract:Adverse biological activities of Thiosemicarbazone (TSC) and Schiff base (SB) Derivatives have been widely studied in rats and in other animal species using different doses, times and routes of administration. However, there are few studies describing changes in some biochemical parameters in vivo which are indicative of oxidative stress in biological systems and of morphological changes of tissues. In this study, the rats were injected subcutaneously with a new Thiosemicarbazone thiazole ring containing a Schiff base (LH) and its Cu(L)2 and Zn(L)2 complexes (25 mg kg−1 body weight) and then sacrificed after 1, 2, 4, 8, 16, 32 and 64 days. The aim of this study was to determine the effect of the new compounds on the serum antioxidant vitamins (A, E, C), selenium (Se), malondialdehyde (MDA) levels, erythrocyte GSH-Px enzyme activity and morphological changes in the liver, kidney and adrenal gland tissues. It was observed that erythrocyte GSH-Px activity, serum MDA and vitamins A, E concentrations were statistically changed (p < 0.02), but serum levels of selenium, and vitamin C were not changed. In conclusion, the parameters measured show that Cu(L)2 caused considerable oxidative stress and Zn(L)2 behaved as an antioxidant. No oxidative stress in LH was observed compared to the control group. Copyright © 2005 John Wiley & Sons, Ltd.
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effects on rat testes of the Thiosemicarbazone Derivative schiff base 4 1 phenylmethylcyclobutane 3 yl 2 2 hydroxybenzylidenehydrazino thiazole and its cadmium ii complex
Cell Biochemistry and Function, 2005Co-Authors: Hakan Oner, Mustafa Karatepe, Fikret Karatas, Jale Oner, Ibrahim Yilmaz, Alaaddin CukurovaliAbstract:The aim of this study was to investigate structural and biochemical changes in testes of rats treated with the Thiosemicarbazone Derivative thiazole ring Schiff base, (4-(1-phenyl-methylcyclobutane-3-yl)-2-(2-hydroxybenzylidene-hydrazino) thiazole (L), and its Cd(II) complex (CdL2). The animals were divided into three groups. Group I was designated as control. The rats in groups II and III were injected subcutaneously with L or CdL2 respectively at 150-mg kg−1 doses at 3-day intervals for 15 days. At the end of the study, blood samples were collected for biochemical analysis, and testes were removed for histological examinations. Serum levels of vitamin A, E and MDA of the L-injected group were similar to the control group. While CdL2 treatment decreased serum vitamin A and E levels, it increased the MDA level compared to other groups. Histologically, the testes structures of L-treated animals were similar to the control. Spermatogenic cells in seminiferous tubules of CdL2-treated animals displayed necrosis. Nuclei of spermatogonia and primary spermatocytes were pyknotic and heterochromatic. Homogenous pink particles were present in place of the spermatids. The interstitial areas were oedematous and intertubular vessels were plugged. In conclusion, the present results indicate that L does not cause biochemical and morphological alterations, but its Cd(II) complex has degenerative effects in normal rat testes. Copyright © 2004 John Wiley & Sons, Ltd.
Ravi Vasanthapuram - One of the best experts on this subject based on the ideXlab platform.
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Butterfly structure of N-Methylisatin-β-Thiosemicarbazone Derivative SCH 16
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and -6
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:E standard antiviral agent Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in JEV (yellow bars) and WNV (green bars) plaques obtained in PS cells with the SCH 16 (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in Den-2 plaques (orange bars) obtained in BHK 21 cells with the Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Note that there was no reduction of in Den-2 plaques was obtained with the SCH 16 in BHK 21 cells. The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and -1
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:E standard antiviral agent Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in JEV (yellow bars) and WNV (green bars) plaques obtained in PS cells with the SCH 16 (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in Den-2 plaques (orange bars) obtained in BHK 21 cells with the Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Note that there was no reduction of in Den-2 plaques was obtained with the SCH 16 in BHK 21 cells. The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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Lane 1-uninfected cell control, lanes 2 and 4 – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at 4 and 10 hours post infection respectively
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:Lanes 3 and 5 – in translation product of RNA obtained from JEV infected PS cells treated with SCH 16 for 4 hours and 10 hours respectively. Note that SCH 16 treatment of JEV infected cells did not show any in vitro translation product at 4 hours post treatment (Lane 3) whilst at 10 hours (Lane 5) a 50 Kda translation product was obtained. Lane M represents molecular weight markers.Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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n methylisatin beta Thiosemicarbazone Derivative sch 16 is an inhibitor of japanese encephalitis virus infection in vitro and in vivo
Virology Journal, 2008Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, Dharmarajan Sriram, Ravi VasanthapuramAbstract:During the early and mid part of 20th century, several reports described the therapeutic effects of N-methylisatin-β-Thiosemicarbazone (MIBT) against pox viruses, Maloney leukemia viruses and recently against HIV. However, their ability to inhibit flavivirus replication has not been investigated. Hence the present study was designed to evaluate the antiviral activity of 14 MIBT Derivatives against Flaviviruses that are prevalent in India such as Japanese Encephalitis Virus (JEV), Dengue-2 (Den-2) and West Nile viruses (WNV). Amongst the fourteen Mannich bases of MIBT Derivatives tested one compound – SCH 16 was able to completely inhibit in vitro Japanese encephalitis virus (JEV) and West Nile virus (WNV) replication. However no antiviral activity of SCH 16 was noted against Den-2 virus replication. This compound was able to inhibit 50% of the plaques (IC50) produced by JEV and WNV at a concentration of 16 μgm/ml (0.000025 μM) and 4 μgm/ml (0.000006 μM) respectively. Furthermore, SCH 16 at a concentration of 500 mg/kg body weight administered by oral route twice daily was able to completely (100%) prevent mortality in mice challenged with 50LD50 JEV by the peripheral route. Our experiments to understand the mechanism of action suggest that SCH 16 inhibited JEV replication at the level of early protein translation. Only one of the 14 isatin Derivatives -SCH 16 exhibited antiviral action on JEV and WNV virus infection in vitro. SCH 16 was also found to completely inhibit JEV replication in vivo in a mouse model challenged peripherally with 50LD50 of the virus. These results warrant further research and development on SCH 16 as a possible therapeutic agent.
Ali A Al-tamimi - One of the best experts on this subject based on the ideXlab platform.
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Antioxidant, antimicrobial, and theoretical studies of the Thiosemicarbazone Derivative Schiff base 2-(2-imino-1-methylimidazolidin-4-ylidene)hydrazinecarbothioamide (IMHC)
Organic and Medicinal Chemistry Letters, 2012Co-Authors: Ahmed A Al-amiery, Yasmien K Al-majedy, Heba H Ibrahim, Ali A Al-tamimiAbstract:Background Adverse antimicrobial activities of Thiosemicarbazone (TSC) and Schiff base Derivatives have widely been studied by using different kinds of microbes, in addition different methods were used to assay the antioxidant activities using DPPH, peroxids, or ntrosyl methods. However, there are no studies describing the synthesis of TSC derived from creatinine. Results In this study, 2-(2-imino-1-methylimidazolidin-4-ylidene)hydrazinecarbothioamide (IMHC) was synthesized by the reaction of creatinine with thiosemicarbazide. The novel molecule was characterized by FT-IR, UV-VIS, and NMR spectra in addition of the elemental analysis. The free radical scavenging ability of the IMHC was determined by it interaction with the stable-free radical 2,2"-diphenyl-1-picrylhydrazyl (or nitric oxide or hydrogen peroxide) and showed encouraging antioxidant activities. Density functional theory calculations of the IMHC performed using molecular structures with optimized geometries. Molecular orbital calculations provide a detailed description of the orbitals, including spatial characteristics, nodal patterns, and the contributions of individual atoms. Highest occupied molecular orbital-lowest unoccupied molecular orbital energies and structures are shown. Conclusions IMHC shows considerable antibacterial and antifungal activities. The free radical scavenging activity of synthesized compound was screened for in vitro antioxidant activity.
Liba Sebastian - One of the best experts on this subject based on the ideXlab platform.
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combination of n methylisatin β Thiosemicarbazone Derivative sch16 with ribavirin and mycophenolic acid potentiates the antiviral activity of sch16 against japanese encephalitis virus in vitro
Letters in Applied Microbiology, 2012Co-Authors: Liba Sebastian, Anita Desai, Dharmarajan Sriram, Perumal Yogeeswari, Shampur Narayan Madhusudana, V RaviAbstract:Aim: To investigate the drug to drug interaction of N-methylisatin-β-Thiosemicarbazone (MIBT) Derivative (SCH16) with ribavirin, mycophenolic acid and pentoxifylline against Japanese encephalitis virus in vitro. Our earlier studies have reported significant antiviral activity of these compounds against Japanese encephalitis virus in vitro and in vivo. Methods and Results: An in vitro drug to drug combination analysis was carried out to investigate whether or not the direct antiviral effect shown by the individual MIBT Derivative could be effectively increased when lower concentrations of two compounds in combination were used. The results of this study showed that the combination of MIBT Derivative (SCH16) with ribavirin or mycophenolic acid significantly enhanced the antiviral activity of SCH16 against JEV in vitro. In contrast, the combination of SCH16 and pentoxifylline resulted in antagonism. Conclusion: The antiviral activity showed by SCH16 was enhanced in the presence of ribavirin and mycophenolic Acid. Significance and Impact of the Study: Studying the synergistic/additive interaction of the compounds in combination would help in lowering the effective concentration so as to overcome the concern of toxicity.
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Butterfly structure of N-Methylisatin-β-Thiosemicarbazone Derivative SCH 16
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and -6
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:E standard antiviral agent Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in JEV (yellow bars) and WNV (green bars) plaques obtained in PS cells with the SCH 16 (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in Den-2 plaques (orange bars) obtained in BHK 21 cells with the Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Note that there was no reduction of in Den-2 plaques was obtained with the SCH 16 in BHK 21 cells. The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and -1
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:E standard antiviral agent Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in JEV (yellow bars) and WNV (green bars) plaques obtained in PS cells with the SCH 16 (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Represents the dose dependent reduction in Den-2 plaques (orange bars) obtained in BHK 21 cells with the Ribavirin (represented as bars). The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. : Note that there was no reduction of in Den-2 plaques was obtained with the SCH 16 in BHK 21 cells. The X axis represents the various concentrations of the compound, Y' axis represents the percent reduction in plaques. The viability of cells is represented as line graph superimposed on the bar diagram on the Y axis. Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.
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Lane 1-uninfected cell control, lanes 2 and 4 – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at 4 and 10 hours post infection respectively
2011Co-Authors: Liba Sebastian, Anita Desai, Madhusudana N Shampur, Yogeeswari Perumal, D Sriram, Ravi VasanthapuramAbstract:Lanes 3 and 5 – in translation product of RNA obtained from JEV infected PS cells treated with SCH 16 for 4 hours and 10 hours respectively. Note that SCH 16 treatment of JEV infected cells did not show any in vitro translation product at 4 hours post treatment (Lane 3) whilst at 10 hours (Lane 5) a 50 Kda translation product was obtained. Lane M represents molecular weight markers.Copyright information:Taken from "N-methylisatin-beta-Thiosemicarbazone Derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection and "http://www.virologyj.com/content/5/1/64Virology Journal 2008;5():64-64.Published online 22 May 2008PMCID:PMC2408923.