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Weidong Zhang - One of the best experts on this subject based on the ideXlab platform.
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integrative metabolic and microbial profiling on patients with spleen Yang Deficiency syndrome
Scientific Reports, 2018Co-Authors: Wu Ye, Weidong Zhang, Xianpeng Zu, Houkai Li, Yiping LiAbstract:Gut microbiota is recognized as an indispensable “metabolic organ” that plays crucial roles in maintaining human health or initiating diseases. Spleen-Yang-Deficiency syndrome (SYDS) is a common syndrome of Traditional Chinese Medicine (TCM) clinic. It is a complex phenotype reflecting the overall changes of metabolism which are mainly caused by digestive disorders. However, little is known about the changes of gut microbiota and metabolism in patients with SYDS, as well as the crosstalk between gut microbiota and host metabolism. In the current study, an integrative metabolic and microbial profiling was performed on plasma, urine and feces from recruited SYDS and healthy individuals by using a LC-QTOFMS-based metabolomic and 16 s rRNA sequencing approaches. Our results showed a potentially significant contribution of gut dysbiosis to the metabolic disorders in SYDS. By integrating the differential gut bacteria with the metabolites, the results revealed some active bacterium of norank_f_CFT112H7, f_lachnospiraceae and bacteroides were closely involved in host mucosal integrity, bile acid metabolism and polysaccharides decomposition. Therefore, our results indicated the probable involvement of gut microbiota in mediating the metabolic changes, which warrants a further investigation on the role of gut microbiota in modulating the pathogenesis of SYDS.
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plasma metabolomics coupled with metaboanalyst and ingenuity pathway analysis characterizes linoleic acid metabolism disorder in patients with spleen Yang Deficiency syndrome
European Journal of Integrative Medicine, 2018Co-Authors: Tao Zhang, Weidong Zhang, Xianpeng Zu, Lirui QiaoAbstract:Abstract Introduction Spleen-Yang-Deficiency syndrome (SYDS) is a hot spot pattern in gastroenterology in traditional Chinese medicine (TCM) clinic and has received increasing attention from clinicians because it is closely related to diseases of diarrhoea, irritable bowel syndrome, myasthenia and gastric cancer, etc. Although the symptom-based diagnostic criteria for SYDS have been suggested, the real causes are still unclear. Methods In this current study, metabolic profile comparisons of plasma for individuals with SYDS were compared with healthy volunteers by using UPLC-QTOFMS technique. Results The results showed that the metabolic profiles for those with SYDS were completely different from those of healthy controls and 15 ions were successfully identified as potential biomarkers. PC (38:4), linoleic acid, 9,10-epoxyoctadecenoic acid and gamma-linolenic acid in linoleic acid metabolism indicated a significant energy metabolism dysfunction in SYDS patients. In addition, other potential biomarkers indicated the patients may also suffer from inflammation, mind disorder and steroid hormone disorders. Conclusions These identified potential biomarkers and proposed related metabolic process show potential for the better understanding of the mechanism of TCM SYDS from a global angle.
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Metabolic profiling study of Yang Deficiency syndrome in hepatocellular carcinoma by h1 NMR and pattern recognition.
Evidence-based Complementary and Alternative Medicine, 2012Co-Authors: Xue-qiang Huang, Qunwei Chen, Genjin Yang, Qing-bo Lang, Juan Du, Weidong Zhang, Changquan LingAbstract:This study proposes a 1H NMR-based metabonomic approach to explore the biochemical characteristics of Yang Deficiency syndrome in hepatocellular carcinoma (HCC) based on serum metabolic profiling. Serum samples from 21 cases of Yang Deficiency syndrome HCC patients (YDS-HCC) and 21 cases of non-Yang Deficiency syndrome HCC patients (NYDS-HCC) were analyzed using 1H NMR spectroscopy and partial least squares discriminant analysis (PLS-DA) was applied to visualize the variation patterns in metabolic profiling of sera from different groups. The differential metabolites were identified and the biochemical characteristics were analyzed. We found that the intensities of six metabolites (LDL/VLDL, isoleucine, lactate, lipids, choline, and glucose/sugars) in serum of Yang Deficiency syndrome patients were lower than those of non-Yang Deficiency syndrome patients. It implies that multiple metabolisms, mainly including lipid, amino acid, and energy metabolisms, are unbalanced or weakened in Yang Deficiency syndrome patients with HCC. The decreased intensities of metabolites including LDL/VLDL, isoleucine, lactate, lipids, choline, and glucose/sugars in serum may be the distinctive metabolic variations of Yang Deficiency syndrome patients with HCC. And these metabolites may be potential biomarkers for diagnosis of Yang Deficiency syndrome in HCC.
Famei Li - One of the best experts on this subject based on the ideXlab platform.
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an integrated plasma and urinary metabonomic study using uhplc ms intervention effects of epimedium koreanum on kidney Yang Deficiency syndrome rats
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Danxue Huang, Jie Yang, Xiumei Lu, Ying Deng, Zhili Xiong, Famei LiAbstract:Abstract A metabonomic strategy based on UHPLC–MS with principal component analysis was developed to investigate the intervention effects of Epimedium koreanum on metabolism characters of ‘Kidney-Yang Deficiency syndrome’ rats. The rats were injected intraperitoneally hydrocortisone once daily for 15 days to simulate ‘Kidney-Yang Deficiency syndrome’ and then administered orally E. koreanum extract once daily for the following 15 days. Plasma and urine samples before hydrocortisone injection, on day 15 of hydrocortisone injection and on days 3, 6, 9, 12, 15 exposed to E. koreanum extract were collected. Significant metabolic disorders were observed in ‘Kidney-Yang Deficiency syndrome’ rats and sixteen potential biomarkers were identified. The disturbed plasma levels of phenylalanine, tryptophan, cholic acid, lysophosphatidylcholines and urinary levels of phenylalanine, hippurate, phenylacetylglycine, N2-succinyl- l -ornithine, creatinine, α-ketoglutarate, citrate, phenol sulfate, indoxyl sulfate, cresol sulfate in model rats were gradually restored to normal after administration of E. koreanum extract, which indicated that E. koreanum had time-dependent recovering effects via regulating oxidant–antioxidant balance, amino acid metabolism, lipid metabolism, energy metabolism, and gut microflora. This work highlights that metabonomics is a promising tool for studying the essence of Chinese medicine's syndrome theory and the action mechanism of traditional Chinese medicine, and provides scientific and reasonable information on safety and efficacy of traditional Chinese medicine.
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metabonomic study on kidney Yang Deficiency syndrome and intervention effects of rhizoma drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry
Talanta, 2011Co-Authors: Xiumei Lu, Zhili Xiong, Jingjing Li, Shuning Zheng, Famei LiAbstract:Abstract This paper was designed to study metabonomic characters of the ‘Kidney-Yang Deficiency syndrome’ induced by high dose of hydrocortisone and the therapeutic effects of Rhizoma Drynariae, classic traditional Chinese medicine (TCM) in treating the syndrome. A urinary metabonomics method based on ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS) was developed. The significant difference in metabolic profiling was observed from model group (hydrocortisone-induced group) compared with the pre-dose group (rats before hydrocortisone inducing) by using the principal components analysis (PCA). The time-dependent regression tendency in Rhizoma Drynariae treatment group (hydrocortisone-induced rats followed by being administered with Rhizoma Drynariae ethanol extracts) from day 3 to 15 was obtained, indicating the time-dependent recovery effect of Rhizoma Drynariae on ‘Kidney-Yang Deficiency syndrome’ rats. Some significantly changed metabolites like phenylalanine, phenylacetylglycine, N 2 -succinyl- l -ornithine, l -proline, creatinine, hippurate and citrate have been identified. These biochemical changes are related to the disturbance in energy metabolism, amino acid metabolism and gut microflora, which are helpful to further understand the ‘Kidney-Yang Deficiency syndrome’ and the therapeutic mechanism of Rhizoma Drynariae. The work shows that the metabonomics method is a valuable tool for studying the essence of Chinese medicine's syndrome theory and therapeutic effect mechanism of TCM.
Qi Wang - One of the best experts on this subject based on the ideXlab platform.
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composition of intestinal microflora associated with Yang Deficiency
Journal of Traditional Chinese Medical Sciences, 2017Co-Authors: Tengjie Huang, Qi Wang, Qizhai Li, Junheng Wang, Fei Yang, Lingru Li, Ji Wang, Yingshuai LiAbstract:Abstract Objective In this study, we investigated the composition of the intestinal microflora associated with Yang-Deficiency and the function-specific members of this microflora. Method Subjects with normal and Yang-deficient constitutions were recruited in Beijing, with 30 subjects in each group. Illumina high-throughput sequencing was used to sequence the DNA of their fecal bacteria in the 16S rRNA V3–V4 region. The species abundance and distribution of the intestinal microflora in each specimen were determined with a statistical analysis of these sequences. We identified an underlying taxonomic trend with nonparametric PCoA and other statistical techniques. Results (1) Subjects with Yang-Deficiency displayed more uneven abundances of the taxa in their intestinal microflora than the subjects with normal Yang; (2) several differences were observed in the proportions of certain bacterial groups between the Yang-deficient and control groups; and (3) the following bacteria differed significantly between the two groups: (i) Brevundimonas, Leuconostoc, Turicibacter, Defluviitaleaceae incertae sedis, Rothia, and Butyricimonas were slightly higher in the Yang-deficient subjects, whereas (ii) Megasphaera, Ruminococcus, and Lachnospira were higher in the normal subjects than in the Yang-deficient subjects. Conclusions The composition of the intestinal microflora is altered in Yang-deficient subjects.
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expression profiling of transcriptome and its associated disease risk in Yang Deficiency constitution of healthy subjects
Evidence-based Complementary and Alternative Medicine, 2016Co-Authors: Ruoxi Yu, Yin Yang, Yingshuai Li, Siqi Li, Qi WangAbstract:Objectives. Differences among healthy subjects and associated disease risks are of substantial interest in clinical medicine. According to the theory of “constitution-disease correlation” in traditional Chinese medicine, we try to find out if there is any connection between intolerance of cold in Yang Deficiency constitution and molecular evidence and if there is any gene expression basis in specific disorders. Methods. Peripheral blood mononuclear cells were collected from Chinese Han individuals with Yang Deficiency constitution ( ) and balanced constitution ( ) (aged 18–28) and global gene expression profiles were determined between them using the Affymetrix HG-U133 Plus 2.0 array. Results. The results showed that when the fold change was ≥1.2 and q ≤ 0.05, 909 genes were upregulated in the Yang Deficiency constitution, while 1189 genes were downregulated. According to our research differential genes found in Yang Deficiency constitution were usually related to lower immunity, metabolic disorders, and cancer tendency. Conclusion. Gene expression disturbance exists in Yang Deficiency constitution, which corresponds to the concept of constitution and gene classification. It also suggests people with Yang Deficiency constitution are susceptible to autoimmune diseases, enteritis, arthritis, metabolism disorders, and cancer, which provides molecular evidence for the theory of “constitution-disease correlation.”
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Changes of endocrine and immune function in subjects of Yang Deficiency constitution
Journal of Chinese Integrative Medicine, 2008Co-Authors: Qi Wang, Jing Dong, Hong-dong Wu, Cheng-yu Wu, Guo-ming Pang, Qi-wei Deng, Jian-xiong ZhaoAbstract:OBJECTIVE: To investigate the changes of endocrine, cyclic nucleotide and immune systems in subjects of Yang Deficiency constitution, and to explore the relationship among characteristics and causes of Yang Deficiency constitution, the physiological and biochemical parameters. METHODS: Based on the diagnostic criteria for the clinical epidemiological investigation, sixty subjects of Yang Deficiency constitution and fifty of normal constitution were selected. Eight milliliters venous blood were taken from overnight fasted subjects at 8:00-9:00. The sera were obtained by centrifugation of the blood at the speed of 3000 r/min for 5 minutes, and they were stored at -70 degrees C until use. The serum levels of corticosterone, cortisol, adrenocorticotrophic hormone (ACTH), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), free triiodothyronine (FT3), free thyroxine (FT4), thyrotropic-stimulating hormone (TSH), interleukin-1beta and interleukin-2 were measured by enzyme-linked immunosorbent assay; the cAMP/cGMP ratio was also computed; and the differences of the above indexes were compared between the two types of subjects. RESULTS: The serum levels of corticosterone, interleukin-1beta, TSH and cAMP/cGMP ratio of Yang Deficiency constitution significantly increased as compared with those of normal constitution, and the serum levels of cortisol, ACTH, cGMP and FT4 of Yang Deficiency constitution significantly decreased in comparison with those of normal constitution. CONCLUSION: Subjects of Yang Deficiency constitution may be not only related to hypothalamic-pituitary-adrenal axis and hypothalamic-pituitary-thyroid axis, but also related to the functional disorders of cyclic nucleotide and immune systems.
Yueguo Gu - One of the best experts on this subject based on the ideXlab platform.
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end to end syndrome differentiation of yin Deficiency and Yang Deficiency in traditional chinese medicine
Computer Methods and Programs in Biomedicine, 2019Co-Authors: Qinan Hu, Tong Yu, Jinghua Li, Qi Yu, Yueguo GuAbstract:Abstract Background and Objective.Yin and Yang, two concepts adapted from classical Chinese philosophy, play a diagnostic role in Traditional Chinese Medicine (TCM). The Yin and Yang in harmonious balance indicate health, whereas imbalances to either side indicate unhealthiness, which may result in diseases. Yin-Yang disharmony is considered to be the cause of pathological changes. Syndrome differentiation of yin-Yang is crucial to clinical diagnosis. It lays a foundation for subsequent medical judgments, including therapeutic methods, and formula, among many others. However, because of the complexities of the mechanisms and manifestations of disease, it is difficult to exactly point out which one, yin or Yang, is disharmonious. There has been inadequate research conducted on syndrome differentiation of yin and Yang from a computational perspective. In this study, we present a computational method, viz. an end-to-end syndrome differentiation of yin Deficiency and Yang Deficiency. Methods.Unlike most previous studies on syndrome differentiation, which use structured datasets, this study takes unstructured texts in medical records as its inputs. It models syndrome differentiation as a task of text classification. This study experiments on two state-of-the-art end-to-end algorithms for text classification, i.e. a classic convolutional neural network (CNN) and fastText. These two systems take the n-grams of several types of tokens as their inputs, including characters, terms, and words. Results.When evaluated on a data set with 7326 modern medical records in TCM, it is observed that CNN and fastText generally give rise to comparable performances. The best accuracy rate of 92.55% comes from the system taking inputs as raw as n-grams of characters. It implies that one can build at least a moderate system for the differentiation of yin Deficiency and Yang Deficiency even if he has no glossary or tokenizer at hand. Conclusions.This study has demonstrated the feasibility of using end-to-end text classification algorithms to differentiate yin Deficiency and Yang Deficiency on unstructured medical records.
Yichang Su - One of the best experts on this subject based on the ideXlab platform.
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Yang Deficiency body constitution acts as a predictor of diabetic retinopathy in patients with type 2 diabetes taichung diabetic body constitution study
Evidence-based Complementary and Alternative Medicine, 2015Co-Authors: Tsai Chung Li, Chiai Tsai, Yachi Wu, Yichang SuAbstract:Objective. Diabetic retinopathy (DR), the most common microvascular complication of diabetes mellitus (DM), can cause severe visual impairment and blindness. To prevent the development of DR, identifying the associated risk factors for patient classification is critical. We conducted a cross-sectional study to determine whether body constitution (BC) is an independent predictor of DR. Method. 673 type 2 DM (T2DM) patients were recruited from a medical center, all received DR examination and body constitution questionnaire to assess BC. Other risk factors for DR were also recorded, including life style, history of diabetes, and blood pressure, etc. Multiple logistic regression analysis was conducted to calculate the odds ratios (ORs) for DR. Results. The prevalence of DR was significantly lower in Yang Deficiency patients compared with non-Yang Deficiency patients (24.69% versus 38.18% P = 0.02). After adjusting for other risk factors, we observed that patients exhibiting Yang Deficiency BC were less likely to present with DR (OR = 0.531; 95% confidence interval = 0.312–0.903, P = 0.018). Conclusion. In addition to traditional risk factors, Yang Deficiency BC might be an independent predictor of DR among T2DM patients and the results can be used as evidence for traditional Chinese medicine patient classification.
