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Valéry Zeitoun – One of the best experts on this subject based on the ideXlab platform.

  • Dating, stratigraphy and taphonomy of the Pleistocene site of Ban Fa Suai II (Northern Thailand): Contributions to the study of paleobiodiversity in Southeast Asia
    Annales de Paléontologie, 2019
    Co-Authors: Valéry Zeitoun, Jeroen Thompson, Winayalai Chinnawut, Régis Debruyne, Stéphane Frère, Lenoble Arnaud, Corentin Bochaton, Kevin Burdette, Jean-baptiste Mallye, Pierre-olivier Antoine

    Abstract:

    Since the initial description of the complex Ailuropoda-Stegodon as a faunal association with biochronological significance for the Southeast Asian area, few sites have provided paleontological data allowing for an extensive documentation of past fauna. Biodiversity and paleo-environmental reconstructions of Pleistocene fauna are still generally based on bone assemblages whose taphonomy and dating are not or badly documented. However, in order to be useful in a paleo-eclogical perspective, the dating of collected assemblages should be associated with periods of times corresponding to climatic episodes. In this study, we provide a detailed stratigraphic, taphonomical, paleontological and ESR dating studies concerning the site of Ban Fa Suai II discovered near the cave of the Monk in northern Thailand. Our results demonstrate the changes in the taxonomic composition of the fauna over time and argue against the use of the currently available regional dataset for paleo-ecological reonstructions.

  • A sustainable review of the Middle Pleistocene benchmark sites including the Ailuropoda–Stegodon faunal complex: The Proboscidean point of view
    Quaternary International, 2016
    Co-Authors: Valéry Zeitoun, Winayalai Chinnawut, Régis Debruyne, Stéphane Frère, Prasit Auetrakulvit

    Abstract:

    The different ecologies, times of extinction and ‘last stand’ of both Elephas and Stegodon in Southeast Asia is covering almost two million years. For Middle Pleistocene, both taxa belong to the complex Ailuropoda-Stegodon. This regional complex is considered to have a chronological significance and further palaeoecological, palaeobiogeographical or biochronological studies are using this assemblage as a benchmark. Nevertheless, such studies do not provide sufficient information regarding site formation and duration to be consistent enough to do so at an appropriate resolution (MIS timescale). Focussing attention on the occurrence of Proboscideans, a critical review of the robustness of the geological, taphonomical and chronological data of Middle Pleistocene faunal assemblages suggests to undertake a deep reappraisal of this “biochronological benchmark”. Recomandations were provided in the 1980s not to use mixtures of faunal assemblages and progress in geochronology for two decades are available. Nevertheless, reviewing Proboscideans suggests the necessity of a severe revision and leads to be very sceptical on the use of the Ailuropoda-Stegodon complex as an ecological marker. Extended to other taxa such a review casts serious doubts on former and current paleoecological modellings and studies.

  • A sustainable review of the Middle Pleistocene benchmark sites including the Ailuropoda-Stegodon faunal complex: The Proboscidean point of view
    Quaternary International, 2016
    Co-Authors: Valéry Zeitoun, Winayalai Chinnawut, Régis Debruyne, Stéphane Frère, Prasit Auetrakulvit

    Abstract:

    Abstract The different ecologies, times of extinction and ‘last stand’ of both Elephas and Stegodon in South-east Asia cover almost two million years. In the Middle Pleistocene, both taxa belong to the complex Ailuropoda–Stegodon. This regional complex is considered to have a chronological significance, and further palaeoecological, palaeobiogeographical or biochronological studies use this assemblage as a benchmark. Nevertheless, such studies do not provide sufficient information regarding site formation and duration to be consistent enough to do so at an appropriate resolution (MIS timescale). Focussing attention on the occurrence of Proboscideans, a critical review of the robustness of the geological, taphonomical and chronological data of Middle Pleistocene faunal assemblages suggests the necessity to undertake a deep reappraisal of this “biochronological benchmark”. Recommendations were provided in the 1980s not to use mixtures of faunal assemblages, and progress in geochronology for two decades is available. Nevertheless, a review of Proboscideans suggests the necessity of a severe revision, leading to scepticism concerning the use of the Ailuropoda–Stegodon complex as an ecological marker. Extended to other taxa, this review casts serious doubts on former and current paleoecological modellings and studies.

Xueshi Huang – One of the best experts on this subject based on the ideXlab platform.

  • cytotoxic fusicoccane type diterpenoids from streptomyces violascens isolated from Ailuropoda melanoleuca feces
    Journal of Natural Products, 2017
    Co-Authors: Dan Zheng, Yi Jiang, Xiu Chen, Xiaodan Qu, Jialiang Zhong, Xiaoxu Bi, Chenglin Jiang, Xueshi Huang

    Abstract:

    Six new fusicoccane-type diterpenoids (2–7) were isolated from the fermentation broth of Streptomyces violascens, which was isolated from Ailuropoda melanoleuca (giant panda) feces. The structures of these new compounds were elucidated by a detailed spectroscopic data and X-ray crystallographic analysis. Compounds 5–7 demonstrated cytotoxicity against five human cancer cell lines, with IC50 values ranging from 3.5 ± 0.7 to 14.1 ± 0.8 μM. Cell adhesion, migration, and invasion assays showed that 6 inhibited the migration and invasion of human hepatocellular carcinoma SMMC7721 cells in a dose-dependent manner. Through further investigation, it was revealed that 6 inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), in addition to down-regulating the expressions of MMP-2 and MMP-9 at both the protein and mRNA levels to influence the migration and invasion of cancer cells.

  • Cytotoxic Fusicoccane-Type Diterpenoids from Streptomyces
    violascens Isolated from Ailuropoda
    melanoleuca Feces
    , 2017
    Co-Authors: Dan Zheng, Yi Jiang, Xiu Chen, Jialiang Zhong, Chenglin Jiang, Li Han, Jiang Liu, Xueshi Huang

    Abstract:

    Six new fusicoccane-type diterpenoids
    (2–7) were isolated from the fermentation
    broth of Streptomyces
    violascens, which was isolated from Ailuropoda melanoleuca (giant panda) feces. The structures of these new compounds were
    elucidated by a detailed spectroscopic data and X-ray crystallographic
    analysis. Compounds 5–7 demonstrated
    cytotoxicity against five human cancer cell lines, with IC50 values ranging from 3.5 ± 0.7 to 14.1 ± 0.8 μM.
    Cell adhesion, migration, and invasion assays showed that 6 inhibited the migration and invasion of human hepatocellular carcinoma
    SMMC7721 cells in a dose-dependent manner. Through further investigation,
    it was revealed that 6 inhibited the enzymatic activity
    of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9
    (MMP-9), in addition to down-regulating the expressions of MMP-2 and
    MMP-9 at both the protein and mRNA levels to influence the migration
    and invasion of cancer cells

  • structure elucidation of four prenylindole derivatives from streptomyces sp isolated from Ailuropoda melanoleuca feces
    Magnetic Resonance in Chemistry, 2013
    Co-Authors: Dan Zheng, Yi Jiang, Xiu Chen, Yiqing Li, Xueshi Huang

    Abstract:

    Four new prenylindole derivatives, (R)-6-(2,3-dihydroxy-3-methylbutyl)indole (1), (R)-6-(2,3-dihydroxy-3-methylbutyl)indolin-2-one (2), and an unseparated mixture of (Z)-6-(4-hydroxy-3-methylbut-2-en-1-yl)indolin-2-one (3a) and (E)-6-(4-hydroxy-3-methylbut-2-en-1-yl)indolin-2-one (3b) with a ratio of 3 : 2, were isolated from the culture broth of a streptomycete isolated from Ailuropoda melanoleuca feces. Their structures were elucidated on the basis of 1D and 2D NMR spectroscopic techniques. The absolute configuration of 1 was determined by Mosher’s method. Copyright © 2013 John Wiley & Sons, Ltd.

Chi-hsiang Huang – One of the best experts on this subject based on the ideXlab platform.

  • Transesophageal Echocardiography Examination in Ailuropoda melanoleuca.
    Asian Journal of Anesthesiology, 2019
    Co-Authors: Yi-chia Wang, Man-ling Wang, Pao-jung Wang, Chi-hsiang Huang

    Abstract:

    Objective: Cardiac function analysis for Ailuropoda melanoleuca, also called giant panda, with transthoracic echocardiography has several limitations, such as limited probe position area and poor echo window. We want to determine the feasibility of transesophageal echocardiography (TEE) for cardiac examination in Ailuropoda melanoleuca. Methods: Eight-year-old male Ailuropoda melanoleuca was anesthetized by intramuscular ketamine and followed by inhalation isoflurane with spontaneous respiration. TEE probe was inserted through mouth. Comprehensive examinations were done by two-dimensional (2D), Doppler method, 3D zoom, and 3D full volume mode for their cardiac evaluation. Results: The structure of heart was similar to human. We found mild mitral regurgitation. Right ventricle and pulmonary artery were free of any organic lesions. We did comprehensive multiplane examination by American Society of Echocardiography/Society of Cardiovascular Anesthesia guideline, but transgastric (TG) and deep TG view were not approachable. 3D reconstruction offered high quality image and quantitative calculations. Complete examinations took less than 30 min. There was no significant hemodynamic change during probe insertion, and no oropharyngeal trauma certified by dentist. Conclusion: TEE is a reasonable semi-invasive tool for cardiac function evaluation. Though some anatomical limitations prohibited comprehensive 2D views, improvements such as 3D images help to give detailed cardiac examination.

  • Transesophageal Echocardiography Examination in Ailuropoda melanoleuca.
    Asian journal of anesthesiology, 2019
    Co-Authors: Yi-chia Wang, Man-ling Wang, Pao-jung Wang, Jun-cheng Guo, Yen-hsu Lai, Wei-zen Sun, Chi-hsiang Huang

    Abstract:

    Objective Cardiac function analysis for Ailuropoda melanoleuca, also called giant panda, with transthoracic echocardiography has several limitations, such as limited probe position area and poor echo window. We want to determine the feasibility of transesophageal echocardiography (TEE) for cardiac examination in Ailuropoda melanoleuca. Methods Eight-year-old male Ailuropoda melanoleuca was anesthetized by intramuscular ketamine and followed by inhalation isofl urane with spontaneous respiration. TEE probe was inserted through mouth. Comprehensive examinations were done by two-dimensional (2D), Doppler method, 3D zoom and 3D full volume mode for their cardiac evaluation. Results The structure of heart was similar to human. We found mild mitral regurgitation. Right ventricle and pulmonary artery were free of any organic lesions. We did comprehensive multiplane examination by American Society of Echocardiography/Society of Cardiovascular Anesthesia guideline, but transgastric (TG) and deep TG view were not approachable. 3D reconstruction offered high quality image and quantitative calculations. Complete examinations took less than 30 minutes. There was no significant hemodynamic change during probe insertion, and no oropharyngeal trauma certifi ed by dentist. Conclusions TEE is a reasonable semi-invasive tool for cardiac function evaluation. Though some anatomical limitations prohibited comprehensive 2D views, improvements such as 3D images help to give detailed cardiac examination.