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W.l.a. Hetterscheid - One of the best experts on this subject based on the ideXlab platform.

  • Amorphophallus adamsensis(Araceae), a new species from Ilocos Norte, Philippines
    Blumea - Biodiversity Evolution and Biogeography of Plants, 2013
    Co-Authors: L.m. Magtoto, D.g. Mones, K.a. Ballada, C.m. Austria, Romeo M. Dizon, Wilfredo V. Alangui, A.a. Reginaldo, W.m. Galvan, K.t. Dizon, W.l.a. Hetterscheid
    Abstract:

    There are ten known species of Amorphophallus in the Philippines, and an eleventh is herein described and named as Amorphophallus adamsensis. It resembles A. dactylifer and A. rostratus. Diagnostic characters of this new species are provided.

  • A review of the White-flowered Amorphophallus (Araceae: Thomsoniae) Species in Sarawak
    The Gardens' Bulletin Singapore, 2010
    Co-Authors: O.c. Boyle, I.b. Ipor, W.l.a. Hetterscheid
    Abstract:

    A review of the white-flowered Amorphophallus species in Sarawak is presented. A total of five species are recognized, four of which belong to the Eburneus Group and are restricted to limestone, and moreover, locally endemic: A. eburneus Bogner (Padawan and Tebedu areas), A. brachyphyllus Hett. (Bau), A. juliae sp. nov. (Merirai) and A. niahensis sp. nov. (Niah). A fifth species, Amorphophallus infundibuliformis Hett., A.Dearden & A.Vogel, of doubtful affinity, is widespread and locally abundant on a variety of substrates excluding limestone. A key to the white-flowered species in Sarawak is presented and all species are illustrated.

  • Notes on the genus Amorphophallus (Araceae) – 13. Evolution of pollen ornamentation and ultrastructure in Amorphophallus and Pseudodracontium
    Grana, 2005
    Co-Authors: R.c.h.j. Van Ham, W.l.a. Hetterscheid, G. Grob, Wim Star, B.j. Van Heuven
    Abstract:

    A strict consensus tree based on chloroplast and nuclear sequences (rbcL, matK, trnL, FLint2) from 46 Amorphophallus species, two Pseudodracontium species and six outgroups is used to develop a hypothesis for the evolution of ornamentation and ectexine ultrastructure in the pollen of Amorphophallus. There are four main clades: an exclusively African, largely psilate clade (‘African clade’), an Asian, largely psilate clade (‘Asian psilate clade’) and an Asian, largely striate clade consisting of a mainly continental SE Asian clade (‘continental SE Asian striate clade’) and one centred in Malesia (‘Malesian striate clade’). Ultrastructure provides a valuable contribution towards understanding pollen ornamentation in Amorphophallus. Pollen with a thin psilate ectexine without dark granules might be plesiomorphic in Amorphophallus. Then the diverse striate type would be derived. Within both striate clades, reversals to the psilate type occur. Striate pollen with psilate caps, which is nested in the continenta...

  • Notes on the genus Amorphophallus (Araceae) — 8
    Review of Palaeobotany and Palynology, 1998
    Co-Authors: R.w.j.m. Van Der Ham, W.l.a. Hetterscheid, B.j. Van Heuven
    Abstract:

    Abstract The pollen of Amorphophallus and Pseudodracontium has been studied with light microscopy and scanning and transmission electron microscopy. Amorphophallus pollen is always inaperturate, has a wide size range, and shows highly diverse exine surfaces. Eight main surface types have been distinguished, most of which are not clear-cut and show considerable infratypic variation. The exine is unusual and consists of a distinct, acetolysis-resistant endexine and a usually nonresistant ectexine. The latter is ultrastructurally diverse by the presence/absence of variously sized, shaped, and distributed dark granules of unknown composition. Comparison with a preliminary subdivision based on macromorphological data indicates that the pollen surface type often supports infrageneric groupings. However, groups considered to be monophyletic sometimes have highly diverse pollen grains. The genus Pseudodracontium, although macromorphologically very near to Amorphophallus, has a clearly distinct pollen surface type.

  • notes on the genus Amorphophallus araceae 8
    Review of Palaeobotany and Palynology, 1998
    Co-Authors: R.w.j.m. Van Der Ham, W.l.a. Hetterscheid, B.j. Van Heuven
    Abstract:

    Abstract The pollen of Amorphophallus and Pseudodracontium has been studied with light microscopy and scanning and transmission electron microscopy. Amorphophallus pollen is always inaperturate, has a wide size range, and shows highly diverse exine surfaces. Eight main surface types have been distinguished, most of which are not clear-cut and show considerable infratypic variation. The exine is unusual and consists of a distinct, acetolysis-resistant endexine and a usually nonresistant ectexine. The latter is ultrastructurally diverse by the presence/absence of variously sized, shaped, and distributed dark granules of unknown composition. Comparison with a preliminary subdivision based on macromorphological data indicates that the pollen surface type often supports infrageneric groupings. However, groups considered to be monophyletic sometimes have highly diverse pollen grains. The genus Pseudodracontium, although macromorphologically very near to Amorphophallus, has a clearly distinct pollen surface type.

Shrirang R. Yadav - One of the best experts on this subject based on the ideXlab platform.

  • Comparative karyological analysis of Indian Amorphophallus (Araceae)
    Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2019
    Co-Authors: Avinash Ramchandra Gholave, Manoj M. Lekhak, Shrirang R. Yadav
    Abstract:

    Comparative karyotypes of 22 accessions (14 species and six varieties) of Indian Amorphophallus (Araceae) are provided to deduce species interrelationships. Chromosome numbers for A. hirsutus, A. n...

  • Reconstruction of molecular phylogeny of closely related Amorphophallus species of India using plastid DNA marker and fingerprinting approaches.
    Physiology and molecular biology of plants : an international journal of functional plant biology, 2016
    Co-Authors: Avinash R. Gholave, Shrirang R. Yadav, Kiran D. Pawar, Vishwas A. Bapat, Jyoti P. Jadhav
    Abstract:

    Plastid DNA markers sequencing and DNA fingerprinting approaches were used and compared for resolving molecular phylogeny of closely related, previously unexplored Amorphophallus species of India. The utility of individual plastid markers namely rbcL, matK, trnH–psbA, trnLC–trnLD, their combined dataset and two fingerprinting techniques viz. RAPD and ISSR were tested for their efficacy to resolves Amorphophallus species into three sections specific clades namely Rhaphiophallus, Conophallus and Amorphophallus. In the present study, sequences of these four plastid DNA regions as well as RAPD and ISSR profiles of 16 Amorphophallus species together with six varieties of two species were generated and analyzed. Maximum likelihood and Bayesian Inference based construction of phylogenetic trees indicated that among the four plastid DNA regions tested individually and their combined dataset, rbcL was found best suited for resolving closely related Amorphophallus species into section specific clades. When analyzed individually, rbcL exhibited better discrimination ability than matK, trnH–psbA, trnLC–trnLD and combination of all four tested plastid markers. Among two fingerprinting techniques used, the resolution of Amorphophallus species using RAPD was better than ISSR and combination of RAPD +ISSR and in congruence with resolution based on rbcL.

  • Cytotaxonomy of some species of Amorphophallus sect. Rhaphiophallus (Schott) Engl. ( Araceae ) of the Indian Subcontinent
    The Nucleus, 2011
    Co-Authors: Manoj M. Lekhak, Shrirang R. Yadav
    Abstract:

    Karyotypes of six species of Amorphophallus sect. Rhaphiophallus were studied and compared. Although all the species have a chromosome number x = 13, they are morphologically very distinct. They can be further differentiated by quantitative parameters of their karyotypes. A triploid number of 2n = 3x = 39 for Amorphophallus longiconnectivus and A. margaritifer, and 2n = 2x = 26, a diploid number for Amorphophallus bhandarensis is reported for the first time. Previously published data on chromosome numbers of A. hohenackeri, A. konkanensis and A. sylvaticus are confirmed. These data add to the information about karyosystematics of this section and could facilitate elucidating the inter-specific relationships.

  • Notes on the genus Amorphophallus (Araceae) — 5. Amorphophallus konkanensis, a new species from India, and taxonomic reflections on Amorphophallus section Rhaphiophallus
    Blumea, 1994
    Co-Authors: W.l.a. Hetterscheid, Shrirang R. Yadav, K. S. Patil
    Abstract:

    A new species of Amorphophallus sect. Rhaphiophallus (Schott) Engl. from SW India is described. The monophyly of and character evolution in sect. Rhaphiophallus is discussed.

Abdulrahman A. Alatar - One of the best experts on this subject based on the ideXlab platform.

  • Revision of Amorphophallus Blume ex Decne. Sect. Amorphophallus (Araceae) in India
    Bangladesh Journal of Plant Taxonomy, 2014
    Co-Authors: V. Abdul Jaleel, M. Sivadasan, Ahmed H. Alfarhan, Jacob Thomas, Abdulrahman A. Alatar
    Abstract:

    Amorphophallus Blume ex Decne. sect. Amorphophallus in India is revised. It is the smallest of the three sections in India with five species, viz. A. hirsutus Teysm. & Binn. , A. kachinensis Engl. & Gehrm., A. longistylus Kurz , A. napalensis (Wall.) Bogner & Mayo and A. paeoniifolius (Dennst.) Nicolson. Amorphophallus paeoniifolius is the widely distributed species in India with two varieties, viz. A. paeoniifolius var. paeoniifolius , and var. campanulatus (Decne.) Sivad. Amorphophallus longistylus is the only species of the section endemic to India. DOI: http://dx.doi.org/10.3329/bjpt.v21i2.21349 Bangladesh J. Plant Taxon. 21 (2): 105-120, 2014 (December)

  • revision of Amorphophallus blume ex decne sect rhaphiophallus schott engl araceae in india
    Bangladesh Journal of Plant Taxonomy, 2011
    Co-Authors: Abdul V Jaleel, M. Sivadasan, Ahmed H. Alfarhan, Jacob Thomas, Abdulrahman A. Alatar
    Abstract:

    The genus Amorphophallus Blume ex Decne. (Araceae), with more than 200 species, is one of the most taxonomically difficult genera of the family, and in fact is one of the moderately understood of all Asian aroid genera. The 11 sections recognised by Engler have been currently reduced to 10, and out of the 10 sections three, viz. Candarum Engl., Conophallus (Schott) Engl. and Rhaphiophallus (Schott) Engl. are represented in India. Rhaphiophallus as recognized now including sect. Synantherias comprises eight species: Amorphophallus bonaccordensis Sivad. & N. Mohanan, A. hohenackeri (Schott) Engl. & Gehrm., A. konkanensis Hett., S. R.Yadav & K. S. Patil, A. longiconnectivus Bogner, A. margaritifer (Roxb.) Kunth, A. mysorensis E. Barnes & C. E. C. Fisch., A. smithsonianus Sivad., and A. sylvaticus (Roxb.) Kunth. Herein A. bhandarensis S. R.Yadav, Kahalkar & Bhuskute is recognized at the new rank of variety, as A. mysorensis var . bhandarensis (S. R. Yadav, Kahalkar & Bhuskute ) Sivad. & Jaleel. The name Amorphophallus sylvaticus (Roxb.) Kunth is lectotypified here. All except A. sylvaticus (also occurring in Sri Lanka) are endemic to India. Keywords: Araceae; Amorphophallus; Rhaphiophallus; India; Endemics. DOI: 10.3329/bjpt.v18i1.7835 Bangladesh J. Plant Taxon. 18 (1): 1-26, 2011 (June)

Chaozhu Yang - One of the best experts on this subject based on the ideXlab platform.

  • Comparative analysis of complete chloroplast genome sequences of four major Amorphophallus species
    Nature Publishing Group, 2019
    Co-Authors: Erxi Liu, Chaozhu Yang, Ying Diao, Nunung Harijati, Jiangdong Liu, Surong Jin, Lingling Zhao
    Abstract:

    Abstract Amorphophallus (Araceae) contains more than 170 species that are mainly distributed in Asia and Africa. Because the bulbs of Amorphophallus are rich in glucomannan, they have been widely used in food, medicine, the chemical industry and so on. To better understand the evolutionary relationships and mutation patterns in the chloroplast genome of Amorphophallus, the complete chloroplast genomes of four species were sequenced. The chloroplast genome sequences of A. albus, A. bulbifer, A. konjac and A. muelleri ranged from 162,853 bp to 167,424 bp. The A. albus chloroplast (cp) genome contains 113 genes, including 79 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The A. bulbifer cp genome contains 111 genes, including 78 protein-coding genes, 29 tRNA genes and 4 rRNA genes. A. muelleri contains 111 and 113 genes, comprising 78 and 80 protein-coding genes, respectively, 29 tRNA genes and 4 rRNA genes. The IR (inverted repeat) region/LSC (long single copy) region and IR/SSC (short single copy) region borders of the four Amorphophallus cp genomes were compared. In addition to some genes being deleted, variations in the copy numbers and intron numbers existed in some genes in the four cp genomes. One hundred thirty-four to 164 SSRs (simple sequence repeats) were detected in the four cp genomes. In addition, the highest mononucleotide SSRs were composed of A and T repeat units, and the majority of dinucleotides were composed of AT and TA. SNPs (single nucleotide polymorphisms) and indels (insertion-deletions) were calculated from coding genes and noncoding genes, respectively. These divergences comprising SSRs, SNPs and indel markers will be useful in testing the maternal inheritance of the chloroplast genome, identifying species differentiation and even in breeding programs. Furthermore, the regression of ndhK was detected from four Amorphophallus cp genomes in our study. Complete cp genome sequences of four Amorphophallus species and other plants were used to perform phylogenetic analyses. The results showed that Amorphophallus was clustered in Araceae, and Amorphophallus was divided into two clades; A. albus and A. konjac were clustered in one clade, and A. bulbifer and A. muelleri were clustered in another clade. Phylogenetic analysis among the Amorphophallus genus was conducted based on matK and rbcL. The phylogenetic trees showed that the relationships among the Amorphophallus species were consistent with their geographical locations. The complete chloroplast genome sequence information for the four Amorphophallus species will be helpful for elucidating Amorphophallus phylogenetic relationships

  • Gene cloning of a neutral ceramidase from the sphingolipid metabolic pathway based on transcriptome analysis of Amorphophallus muelleri
    PloS one, 2018
    Co-Authors: Lin Zhong, Erxi Liu, Chaozhu Yang, Ying Diao, Nunung Harijati, Jiangdong Liu, Surong Jin
    Abstract:

    Amorphophallus is a perennial herbaceous plant species mainly distributed in the tropics or subtropics of Asia and Africa. It has been used as a traditional medicine for a long time and now is utilized for the pharmaceutical, chemical and agriculture industries as a valued economic crop. Recently, Amorphophallus has attracted tremendous interest because of its high ceramide content. However, the breeding and genome studies are severely limited by the arduous whole genome sequencing of Amorphophallus. In this study, the transcriptome data of A. muelleri was obtained by utilizing the high-throughput Illumina sequencing platform. Based on this information, the majority of the significant genes involved in the proposed sphingolipid metabolic pathway were identified. Then, the full-length neutral ceramidase cDNA was obtained with the help of its candidate transcripts, which were acquired from the transcriptome data. Furthermore, we demonstrated that this neutral ceramidase was a real ceramidase by eukaryotic expression in the yeast double knockout mutant Δypc1 Δydc1, which lacks the ceramidases-dihydroCDase (YDC1p), phytoCDase (YPC1p). In addition, the biochemical characterization of purified A. muelleri ceramidase (AmCDase) exhibited classical Michaelis-Menten kinetics with an optimal activity ranging from pH 6.5 to 8.0. Based on our knowledge, this study is the first to report the related information of the neutral ceramidase in Amorphophallus. All datasets can provide significant information for related studies, such as gene expression, genetic improvement and application on breeding in Amorphophallus.

  • development of microsatellite markers by transcriptome sequencing in two species of Amorphophallus araceae
    BMC Genomics, 2013
    Co-Authors: Xingfei Zheng, Ying Diao, Cheng Pan, Yongning You, Chaozhu Yang
    Abstract:

    Amorphophallus is a genus of perennial plants widely distributed in the tropics or subtropics of West Africa and South Asia. Its corms contain a high level of water-soluble glucomannan; therefore, it has long been used as a medicinal herb and food source. Genetic studies of Amorphophallus have been hindered by a lack of genetic markers. A large number of molecular markers are required for genetic diversity study and improving disease resistance in Amorphophallus. Here, we report large scale of transcriptome sequencing of two species: Amorphophallus konjac and Amorphophallus bulbifer using deep sequencing technology, and microsatellite (SSR) markers were identified based on these transcriptome sequences. cDNAs of A. konjac and A. bulbifer were sequenced using Illumina HiSeq™ 2000 sequencing technology. A total of 135,822 non-redundant unigenes were assembled from about 9.66 gigabases, and 19,596 SSRs were identified in 16,027 non-redundant unigenes. Di-nucleotide SSRs were the most abundant motif (61.6%), followed by tri- (30.3%), tetra- (5.6%), penta- (1.5%), and hexa-nucleotides (1%) repeats. The top di- and tri-nucleotide repeat motifs included AG/CT (45.2%) and AGG/CCT (7.1%), respectively. A total of 10,754 primer pairs were designed for marker development. Of these, 320 primers were synthesized and used for validation of amplification and assessment of polymorphisms in 25 individual plants. The total of 275 primer pairs yielded PCR amplification products, of which 205 were polymorphic. The number of alleles ranged from 2 to 14 and the polymorphism information content valued ranged from 0.10 to 0.90. Genetic diversity analysis was done using 177 highly polymorphic SSR markers. A phenogram based on Jaccard’s similarity coefficients was constructed, which showed a distinct cluster of 25 Amorphophallus individuals. A total of 10,754 SSR markers have been identified in Amorphophallus using transcriptome sequencing. One hundred and seventy-seven polymorphic markers were successfully validated in 25 individuals. The large number of genetic markers developed in the present study should contribute greatly to research into genetic diversity and germplasm characterization in Amorphophallus.

Ying Diao - One of the best experts on this subject based on the ideXlab platform.

  • Screening of the Candidate DNA Barcodes for Three Important Amorphophallus Species Identification
    Agronomy, 2020
    Co-Authors: Chufeng Zhao, Erxi Liu, Nunung Harijati, Surong Jin, Xuan She, Teng Cheng, Ying Diao
    Abstract:

    Amorphophallus is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. Amorphophallus has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to Amorphophallus and closely related species. This study used six barcodes, namely ITS2, matK, rcbL, nad1, trnH-psbA, and trnL-trnF, to evaluate their identification ability for three important Amorphophallus species, including A. konjac, A. albus, and A. muelleri. We recommend that trnH-psbA can be applied to the Amorphophallus trade, quickly identify the purity of A. konjac and A. albus and distinguish A. muelleri from its related species for A. konjac and A. albus genetic improvement.

  • Comparative analysis of complete chloroplast genome sequences of four major Amorphophallus species
    Nature Publishing Group, 2019
    Co-Authors: Erxi Liu, Chaozhu Yang, Ying Diao, Nunung Harijati, Jiangdong Liu, Surong Jin, Lingling Zhao
    Abstract:

    Abstract Amorphophallus (Araceae) contains more than 170 species that are mainly distributed in Asia and Africa. Because the bulbs of Amorphophallus are rich in glucomannan, they have been widely used in food, medicine, the chemical industry and so on. To better understand the evolutionary relationships and mutation patterns in the chloroplast genome of Amorphophallus, the complete chloroplast genomes of four species were sequenced. The chloroplast genome sequences of A. albus, A. bulbifer, A. konjac and A. muelleri ranged from 162,853 bp to 167,424 bp. The A. albus chloroplast (cp) genome contains 113 genes, including 79 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The A. bulbifer cp genome contains 111 genes, including 78 protein-coding genes, 29 tRNA genes and 4 rRNA genes. A. muelleri contains 111 and 113 genes, comprising 78 and 80 protein-coding genes, respectively, 29 tRNA genes and 4 rRNA genes. The IR (inverted repeat) region/LSC (long single copy) region and IR/SSC (short single copy) region borders of the four Amorphophallus cp genomes were compared. In addition to some genes being deleted, variations in the copy numbers and intron numbers existed in some genes in the four cp genomes. One hundred thirty-four to 164 SSRs (simple sequence repeats) were detected in the four cp genomes. In addition, the highest mononucleotide SSRs were composed of A and T repeat units, and the majority of dinucleotides were composed of AT and TA. SNPs (single nucleotide polymorphisms) and indels (insertion-deletions) were calculated from coding genes and noncoding genes, respectively. These divergences comprising SSRs, SNPs and indel markers will be useful in testing the maternal inheritance of the chloroplast genome, identifying species differentiation and even in breeding programs. Furthermore, the regression of ndhK was detected from four Amorphophallus cp genomes in our study. Complete cp genome sequences of four Amorphophallus species and other plants were used to perform phylogenetic analyses. The results showed that Amorphophallus was clustered in Araceae, and Amorphophallus was divided into two clades; A. albus and A. konjac were clustered in one clade, and A. bulbifer and A. muelleri were clustered in another clade. Phylogenetic analysis among the Amorphophallus genus was conducted based on matK and rbcL. The phylogenetic trees showed that the relationships among the Amorphophallus species were consistent with their geographical locations. The complete chloroplast genome sequence information for the four Amorphophallus species will be helpful for elucidating Amorphophallus phylogenetic relationships

  • Gene cloning of a neutral ceramidase from the sphingolipid metabolic pathway based on transcriptome analysis of Amorphophallus muelleri
    PloS one, 2018
    Co-Authors: Lin Zhong, Erxi Liu, Chaozhu Yang, Ying Diao, Nunung Harijati, Jiangdong Liu, Surong Jin
    Abstract:

    Amorphophallus is a perennial herbaceous plant species mainly distributed in the tropics or subtropics of Asia and Africa. It has been used as a traditional medicine for a long time and now is utilized for the pharmaceutical, chemical and agriculture industries as a valued economic crop. Recently, Amorphophallus has attracted tremendous interest because of its high ceramide content. However, the breeding and genome studies are severely limited by the arduous whole genome sequencing of Amorphophallus. In this study, the transcriptome data of A. muelleri was obtained by utilizing the high-throughput Illumina sequencing platform. Based on this information, the majority of the significant genes involved in the proposed sphingolipid metabolic pathway were identified. Then, the full-length neutral ceramidase cDNA was obtained with the help of its candidate transcripts, which were acquired from the transcriptome data. Furthermore, we demonstrated that this neutral ceramidase was a real ceramidase by eukaryotic expression in the yeast double knockout mutant Δypc1 Δydc1, which lacks the ceramidases-dihydroCDase (YDC1p), phytoCDase (YPC1p). In addition, the biochemical characterization of purified A. muelleri ceramidase (AmCDase) exhibited classical Michaelis-Menten kinetics with an optimal activity ranging from pH 6.5 to 8.0. Based on our knowledge, this study is the first to report the related information of the neutral ceramidase in Amorphophallus. All datasets can provide significant information for related studies, such as gene expression, genetic improvement and application on breeding in Amorphophallus.

  • development of microsatellite markers by transcriptome sequencing in two species of Amorphophallus araceae
    BMC Genomics, 2013
    Co-Authors: Xingfei Zheng, Ying Diao, Cheng Pan, Yongning You, Chaozhu Yang
    Abstract:

    Amorphophallus is a genus of perennial plants widely distributed in the tropics or subtropics of West Africa and South Asia. Its corms contain a high level of water-soluble glucomannan; therefore, it has long been used as a medicinal herb and food source. Genetic studies of Amorphophallus have been hindered by a lack of genetic markers. A large number of molecular markers are required for genetic diversity study and improving disease resistance in Amorphophallus. Here, we report large scale of transcriptome sequencing of two species: Amorphophallus konjac and Amorphophallus bulbifer using deep sequencing technology, and microsatellite (SSR) markers were identified based on these transcriptome sequences. cDNAs of A. konjac and A. bulbifer were sequenced using Illumina HiSeq™ 2000 sequencing technology. A total of 135,822 non-redundant unigenes were assembled from about 9.66 gigabases, and 19,596 SSRs were identified in 16,027 non-redundant unigenes. Di-nucleotide SSRs were the most abundant motif (61.6%), followed by tri- (30.3%), tetra- (5.6%), penta- (1.5%), and hexa-nucleotides (1%) repeats. The top di- and tri-nucleotide repeat motifs included AG/CT (45.2%) and AGG/CCT (7.1%), respectively. A total of 10,754 primer pairs were designed for marker development. Of these, 320 primers were synthesized and used for validation of amplification and assessment of polymorphisms in 25 individual plants. The total of 275 primer pairs yielded PCR amplification products, of which 205 were polymorphic. The number of alleles ranged from 2 to 14 and the polymorphism information content valued ranged from 0.10 to 0.90. Genetic diversity analysis was done using 177 highly polymorphic SSR markers. A phenogram based on Jaccard’s similarity coefficients was constructed, which showed a distinct cluster of 25 Amorphophallus individuals. A total of 10,754 SSR markers have been identified in Amorphophallus using transcriptome sequencing. One hundred and seventy-seven polymorphic markers were successfully validated in 25 individuals. The large number of genetic markers developed in the present study should contribute greatly to research into genetic diversity and germplasm characterization in Amorphophallus.