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Abdolreza Mohamadnia - One of the best experts on this subject based on the ideXlab platform.
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Multi-Gene Expression in Anthracosis of the Lungs as One of the Risk Factors for Non-Small Cell Lung Cancer
Asian Pacific journal of cancer prevention : APJCP, 2017Co-Authors: Hamidreza Jamaati, Arda Kiani, Atefeh Abedini, Naghmeh Bahrami, Payam Tabarsi, Adnan Khosravi, Rahim Ahmadi, Somayeh Sharifynia, Abdolreza MohamadniaAbstract:Background: Anthracosis of the lung occurs due to the deposition of carbon and silica in the mucosa and submucosa, manifested as black lesions. The association of Anthracosis with lung cancer has remained to be clearly elucidated The current study aimed to assess the P16, CDH1 and LUNX genes expression level to evaluate the association of anthracotic lesions in the lungs with the occurrence of non-small cell lung cancer. Methods: Forty biopsy samples were taken from the center and 40 from the margins of black anthracotic lesions in the lungs; RNA was extracted from the samples and cDNA was synthesized. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of P16, CDH1 and LUNX genes. All steps were performed in triplicate. Results: A significant reduction in P16 gene expression was noted at the center compared to the margins of the lesions (P
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multi gene expression in Anthracosis of the lungs as one of the risk factors for non small cell lung cancer
Asian Pacific Journal of Cancer Prevention, 2017Co-Authors: Hamidreza Jamaati, Arda Kiani, Atefeh Abedini, Naghmeh Bahrami, Payam Tabarsi, Adnan Khosravi, Rahim Ahmadi, Somayeh Sharifynia, Abdolreza MohamadniaAbstract:Background: Anthracosis of the lung occurs due to the deposition of carbon and silica in the mucosa and submucosa, manifested as black lesions. The association of Anthracosis with lung cancer has remained to be clearly elucidated The current study aimed to assess the P16, CDH1 and LUNX genes expression level to evaluate the association of anthracotic lesions in the lungs with the occurrence of non-small cell lung cancer. Methods: Forty biopsy samples were taken from the center and 40 from the margins of black anthracotic lesions in the lungs; RNA was extracted from the samples and cDNA was synthesized. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of P16, CDH1 and LUNX genes. All steps were performed in triplicate. Results: A significant reduction in P16 gene expression was noted at the center compared to the margins of the lesions (P<0.001). expression level of CDH1 at the center of lesions was significantly lower than margins (P<0.001). However, LUNX gene had significantly higher expressionlevel at the center compared to margins (P<0.001). Conclusion: Decreased expression of P16 and CDH1 and increased expression of LUNX tumor genes were noted at the center of anthracotic lesions. Significant increase in expression of LUNX gene in NSCLC indicates an association between Anthracosis and NSCLC, according to which, anthracotic patients may carry a high risk for NSCLC.
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Surveying the Expression of CDH1 and EGFR Genes in Patients with Anthracosis and Its Relationship with Lung Carcinoma
Asian Pacific Journal of Cancer Care, 2017Co-Authors: Zahra Izadian, Naghmeh Bahrami, Tahereh Naji, Abdolreza MohamadniaAbstract:Introduction: Anthracosis is known as the black lung disease and studies have shown relationships between the disease and lung carcinoma. In addition, the expression of CDH1 and EGFR genes can be used as the prognostic of Anthracosis. The present study surveys the expression of CDH1 and EGFR genes in Anthracosis patients and its relationship with non-small cells lung carcinoma. Methodology: Thirty Anthracosis patients diagnosed by a specialist participated in the study. The subjects were asked to sign an informed letter of consent and then, to examine the expression of CDH1 and EGFR genes through real-time PCR, tissue samples were collected. Afterward, the relationship of expression of the biomarkers with tumor staging and cancer progress was examined. Results: Mean age of the patients was 48±10.5 years. As the reference gene, 18sRNA was adopted and comparison of mean CTs at the center and edges of lesions showed no significant difference. As to CDH1 gene at the center of lesions, 12 cases out of 30 were positive, which indicates 40% sensitivity; while at the edges, 20 cases out 30 were positive. Comparison of the positive rates of CDH1 gene at the center and edges of the lesions indicated a significant difference (P-value
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surveying the expression of cdh1 and egfr genes in patients with Anthracosis and its relationship with lung carcinoma
Asian Pacific Journal of Cancer Care, 2017Co-Authors: Zahra Izadian, Naghmeh Bahrami, Tahereh Naji, Abdolreza MohamadniaAbstract:Introduction: Anthracosis is known as the black lung disease and studies have shown relationships between the disease and lung carcinoma. In addition, the expression of CDH1 and EGFR genes can be used as the prognostic of Anthracosis. The present study surveys the expression of CDH1 and EGFR genes in Anthracosis patients and its relationship with non-small cells lung carcinoma. Methodology: Thirty Anthracosis patients diagnosed by a specialist participated in the study. The subjects were asked to sign an informed letter of consent and then, to examine the expression of CDH1 and EGFR genes through real-time PCR, tissue samples were collected. Afterward, the relationship of expression of the biomarkers with tumor staging and cancer progress was examined. Results: Mean age of the patients was 48±10.5 years. As the reference gene, 18sRNA was adopted and comparison of mean CTs at the center and edges of lesions showed no significant difference. As to CDH1 gene at the center of lesions, 12 cases out of 30 were positive, which indicates 40% sensitivity; while at the edges, 20 cases out 30 were positive. Comparison of the positive rates of CDH1 gene at the center and edges of the lesions indicated a significant difference (P-value<0.001). Moreover and with regard to EGFR gene at the center of lesions, 19 cases out 30 were positive (sensitivity = 63.3%) and five cases out of 30 were positive at the edges of lesions (control). There was also a significant difference between the positive cases of EGFR gene at the center and edges of the lesions (P-value<0.001). Clearly, the positive cases of CDH1 at the edges were higher than that at the center, while in the case of EGFR gene, positive cases at the center were higher than that at the edges. It is notable that to obtain results with higher accuracy entails increasing the sensitivity level. Following similar studies and to have higher accuracy, the experiments were carried out with three iterations. Conclusion: In general, the results indicated a sort of relationship between Anthracosis and lung carcinoma. To prove such relationship on a more solid ground, more studies with larger number of subjects are needed. Keywords: Anthracosis- lung carcinoma- gene expression- CDH1 Biomarker- EGFR biomarker
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relationship with lung carcinoma in the patients with Anthracosis by detection of expression of p16 and muc1 genes
Asian Pacific Journal of Cancer Care, 2017Co-Authors: Mona Ghashghaee, Naghmeh Bahrami, Tahereh Naji, Abdolreza MohamadniaAbstract:Introduction and objective: Anthracosis is a pneumoconiosis caused by coal dust. The term “pneumoconiosis” refers to an occupational lung disease caused by long-term inhalation of dust. To achieve more efficient diagnosis and treatment methods, changes in the expression of MUC1 and P16 genes in the patients with Anthracosis and their relationship with none small cells lung carcinoma were examined. Methodology: Thirty Anthracosis samples (center of the lesion) and thirty healthy samples (edges of the lesion) were collected from Anthracosis patients diagnosed by a specialist. The patients signed a written letter of consent beforehand. The expression of MUC1 and P19 biomarkers was measured through Real-Time PCR. The relationship of the expression of biomarkers with tumor staging and lung carcinoma stage was examined. Results: The two groups in the study were compared using t-test. P16 marker was positive at the center and edges of lesions in seven (out of 30) and 18 (out of 30) cases respectively. There was a significant difference between positive P16 markers at the center and edges of the lesions (p-value<0.001). MUC1 marker was positive at the center of lesions in 21 cases out of 30 (sensitivity = 70%) and it was positive at the edges of lesions in seven cases out of 30. In terms lesions (p-value<0.001). Conclusion: The results indicated a relationship between Anthracosis and lung carcinoma. Clearly, the positive cases of P16 genes at the edges of lesions were higher than that at the center; while the positive cases of MCU1 gene at the center of lesions were higher than that at the edges. Further studies with larger sample groups are needed to provide more evidence. Keywords: Anthracosis disease- lung carcinoma- MUI1 Biomarker- P16 Biomarker
Arda Kiani - One of the best experts on this subject based on the ideXlab platform.
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the utility of elastography during ebus tbna in a population with a high prevalence of Anthracosis
Clinical Respiratory Journal, 2020Co-Authors: Atefeh Abedini, Fatemeh Razavi, Mehrdad Farahani, Marzieh Hashemi, Habib Emami, Forouzan Mohammadi, Arda KianiAbstract:BACKGROUND Ultrasound elastography, is a pioneer sonographic modality that is conducted during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in order to increase the accuracy of sampling location. The current study aims to evaluate the usefulness of elastography during EBUS-TBNA in a population with a high prevalence of Anthracosis. METHODS This prospective single-blinded study was performed on 69 lymph nodes (LNs) of patients with mediastinal lymphadenopathy undergoing EBUS-TBNA and EBUS-elastography from October 2017 to July 2018. The stiffness level of the tissue was translated into a color to demonstrate the hardness of tissue. Blue and total areas of each section were measured to calculate the hardness of each LN. RESULTS Sixty-nine LNs were evaluated by elastography. Twenty percent of LNs were malignant. There was a statistical difference between malignant and non-malignant nodes based on color dominancy (P = 0.032). However, with the exclusion of Anthracosis nodes from the analysis, the difference was more significant (P < 0.001). Moreover, when the blue dominancy was used as the predictor of malignancy or Anthracosis, the results showed a significant correlation (P < 001). CONCLUSION The usefulness of elastography in selecting the hardest area of tissue that is appropriate for diagnosing diseases has been proven previously. Since in countries with a high prevalence of Anthracosis, blue color achieved using elastography predicts either malignancy or Anthracosis so, cases with blue dominancy of LNs in elastography and the white color in the EBUS-TBNA indicate Anthracosis-caused calcification should be reconsidered.
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The utility of elastography during EBUS‐TBNA in a population with a high prevalence of Anthracosis
The clinical respiratory journal, 2020Co-Authors: Atefeh Abedini, Fatemeh Razavi, Mehrdad Farahani, Marzieh Hashemi, Habib Emami, Forouzan Mohammadi, Arda KianiAbstract:BACKGROUND Ultrasound elastography, is a pioneer sonographic modality that is conducted during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in order to increase the accuracy of sampling location. The current study aims to evaluate the usefulness of elastography during EBUS-TBNA in a population with a high prevalence of Anthracosis. METHODS This prospective single-blinded study was performed on 69 lymph nodes (LNs) of patients with mediastinal lymphadenopathy undergoing EBUS-TBNA and EBUS-elastography from October 2017 to July 2018. The stiffness level of the tissue was translated into a color to demonstrate the hardness of tissue. Blue and total areas of each section were measured to calculate the hardness of each LN. RESULTS Sixty-nine LNs were evaluated by elastography. Twenty percent of LNs were malignant. There was a statistical difference between malignant and non-malignant nodes based on color dominancy (P = 0.032). However, with the exclusion of Anthracosis nodes from the analysis, the difference was more significant (P
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Diagnostic triad of pulmonary anthracofibrosis in spiral CT scan - a retrospective study.
Polish journal of radiology, 2019Co-Authors: Shahram Kahkouee, Arda Kiani, Shaghayegh S Khabbaz, Elham Keshavarz, Ghazal Hajinasrollah, Amine Ghaffari AnvarAbstract:Anthracosis, a form of pneumoconiosis commonly caused by air pollution and other environmental factors, is a new entity in respiratory disorders. Bronchoscopy and transbronchial lung biopsy (TBLB) are the gold standard of diagnosis. Herein, we evaluated the results of bronchoscopy and chest computed tomography (CT) scans of 187 anthracotic patients. Between April 2016 and April 2017, 187 cases (99 males, mean age 65 ± 10.2 years) who underwent flexible bronchoscopy and TBLB for various indications were considered for this study. CT examinations of these patients were reported as "blind to bronchoscopy results" by two experienced board-certified radiologists. According to the results of bronchoscopy and TBLB, 100 patients were diagnosed as anthracotic. CT scans confirmed 71 of these cases as Anthracosis. Sensitivity, specificity, PPV, NPV, and accuracy of hyperdense non-calcified mediastinal and hilar lymph nodes, known as "brilliant lymph nodes", compared to bronchoscopy were 55%, 92%, 89%, 64%, and 72%, respectively (CI: 95%). Also, there was a positive correlation between Anthracosis and brilliant lymph nodes (p-value = 0). Analysis of hyper-attenuated lung with bronchoscopy estimated sensitivity, specificity, PPV, NPV, and accuracy gave 41%, 94%, 89%, 58%, and 65%, respectively (CI: 95%). The specificity of brilliant lymph nodes, hyper-attenuated lung, and multi-segmental atelectasis as a diagnostic "triad of Anthracosis" was 100%. Based on our analyses, the triad of brilliant lymph nodes, hyper-attenuated lung, and multi-segmental atelectasis was identified as a reliable set of imaging findings for the diagnosis of Anthracosis.
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Determination of CD4, CD8, and IL-8 levels in serum and bronchoalveolar lavage fluid of Anthracosis patients
Biomedical and Biotechnology Research Journal (BBRJ), 2018Co-Authors: Arda Kiani, Mehdi Ramazanpour, Fatemeh Razavi, Hamidreza Jamaati, Esmaeil Mortaz, Atefeh AbediniAbstract:Background: Anthracosis is a chronic pulmonary disease with black pigmentation of the bronchial mucosa, caused by carbon accumulation in the lungs, and in some cases, associated with pulmonary tuberculosis (TB). The purpose of this study is to evaluate the serum and bronchoalveolar lavage (BAL) fluid levels of the CD4/CD8 ratio and interleukin 8 (IL-8), as the potential diagnosis and prognosis biomarkers for Anthracosis. Methods: Of 60 patients, referred to the Masih Daneshvari Hospital, 30 Anthracosis patients, confirmed by bronchoscopy, were included as the case group. BAL sample and blood samples were collected from all individuals and sent to the immunology laboratory. Patients with no Anthracosis on bronchoscopy, and suspected to have TB, were included in the control group. In addition, BAL samples were used for BK-polymerase chain reaction. Results: In this case–control study, 30 Anthracosis patients and 30 controls were investigated. There were no significant differences in IL-8 of patients with Anthracosis, compared to others. There were significant differences of the CD4/CD8 ratio in BAL fluid, between Anthracosis cases and the control group (1.01 ± 0.77 vs. 2.41 ± 3.50; P = 0.04), and significant differences were seen in serum levels of two groups (P = 0.02). The rate of the confirmed pulmonary TB which was 88.9% in patients with Anthracosis was significantly higher than the control group. Conclusion: These outcomes suggest that changes in the CD4/C8 levels in serum and BAL fluid may have a leading role in the diagnosis or prognosis of Anthracosis. In addition, due to the strong association of Anthracosis and pulmonary TB, TB should be considered in patients with Anthracosis, which in turn can eventuate to the early distinguish and cure of the patients.
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Multi-Gene Expression in Anthracosis of the Lungs as One of the Risk Factors for Non-Small Cell Lung Cancer
Asian Pacific journal of cancer prevention : APJCP, 2017Co-Authors: Hamidreza Jamaati, Arda Kiani, Atefeh Abedini, Naghmeh Bahrami, Payam Tabarsi, Adnan Khosravi, Rahim Ahmadi, Somayeh Sharifynia, Abdolreza MohamadniaAbstract:Background: Anthracosis of the lung occurs due to the deposition of carbon and silica in the mucosa and submucosa, manifested as black lesions. The association of Anthracosis with lung cancer has remained to be clearly elucidated The current study aimed to assess the P16, CDH1 and LUNX genes expression level to evaluate the association of anthracotic lesions in the lungs with the occurrence of non-small cell lung cancer. Methods: Forty biopsy samples were taken from the center and 40 from the margins of black anthracotic lesions in the lungs; RNA was extracted from the samples and cDNA was synthesized. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of P16, CDH1 and LUNX genes. All steps were performed in triplicate. Results: A significant reduction in P16 gene expression was noted at the center compared to the margins of the lesions (P
Atefeh Abedini - One of the best experts on this subject based on the ideXlab platform.
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the utility of elastography during ebus tbna in a population with a high prevalence of Anthracosis
Clinical Respiratory Journal, 2020Co-Authors: Atefeh Abedini, Fatemeh Razavi, Mehrdad Farahani, Marzieh Hashemi, Habib Emami, Forouzan Mohammadi, Arda KianiAbstract:BACKGROUND Ultrasound elastography, is a pioneer sonographic modality that is conducted during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in order to increase the accuracy of sampling location. The current study aims to evaluate the usefulness of elastography during EBUS-TBNA in a population with a high prevalence of Anthracosis. METHODS This prospective single-blinded study was performed on 69 lymph nodes (LNs) of patients with mediastinal lymphadenopathy undergoing EBUS-TBNA and EBUS-elastography from October 2017 to July 2018. The stiffness level of the tissue was translated into a color to demonstrate the hardness of tissue. Blue and total areas of each section were measured to calculate the hardness of each LN. RESULTS Sixty-nine LNs were evaluated by elastography. Twenty percent of LNs were malignant. There was a statistical difference between malignant and non-malignant nodes based on color dominancy (P = 0.032). However, with the exclusion of Anthracosis nodes from the analysis, the difference was more significant (P < 0.001). Moreover, when the blue dominancy was used as the predictor of malignancy or Anthracosis, the results showed a significant correlation (P < 001). CONCLUSION The usefulness of elastography in selecting the hardest area of tissue that is appropriate for diagnosing diseases has been proven previously. Since in countries with a high prevalence of Anthracosis, blue color achieved using elastography predicts either malignancy or Anthracosis so, cases with blue dominancy of LNs in elastography and the white color in the EBUS-TBNA indicate Anthracosis-caused calcification should be reconsidered.
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The utility of elastography during EBUS‐TBNA in a population with a high prevalence of Anthracosis
The clinical respiratory journal, 2020Co-Authors: Atefeh Abedini, Fatemeh Razavi, Mehrdad Farahani, Marzieh Hashemi, Habib Emami, Forouzan Mohammadi, Arda KianiAbstract:BACKGROUND Ultrasound elastography, is a pioneer sonographic modality that is conducted during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in order to increase the accuracy of sampling location. The current study aims to evaluate the usefulness of elastography during EBUS-TBNA in a population with a high prevalence of Anthracosis. METHODS This prospective single-blinded study was performed on 69 lymph nodes (LNs) of patients with mediastinal lymphadenopathy undergoing EBUS-TBNA and EBUS-elastography from October 2017 to July 2018. The stiffness level of the tissue was translated into a color to demonstrate the hardness of tissue. Blue and total areas of each section were measured to calculate the hardness of each LN. RESULTS Sixty-nine LNs were evaluated by elastography. Twenty percent of LNs were malignant. There was a statistical difference between malignant and non-malignant nodes based on color dominancy (P = 0.032). However, with the exclusion of Anthracosis nodes from the analysis, the difference was more significant (P
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differential diagnosis of tuberculosis sarcoidosis and Anthracosis by cd8 cd3 and cd4 levels by flow cytometry
School of Medicine Students' Journal, 2019Co-Authors: Nazanin Akbarifazli, Atefeh Abedini, Esmail MortazAbstract:Background: Determination of a desirable diagnostic tests is an issue of importance especially to differentiate between tuberculosis) TB(, sarcoidosis, and Anthracosis. The purpose of the present study was to determine the differential diagnosis of tuberculosis, sarcoidosis and Anthracosis with CD8, CD3, and CD4 by flow-cytometry. Materials and Methods: In this descriptive cross-sectional comparative survey, 40 consecutive patients attending to Masih Daneshvari Hospital in Tehran were enrolled and CD4/CD8 ratio, CD8, CD3, and CD4 were determined by flow-cytometry and compared across patients with three diseases including tuberculosis, sarcoidosis, and Anthracosis.Results: The results demonstrated that CD4 was significantly higher in Anthracosis cases (P<0.007) and the CD8 was significantly higher in patients with TB (P<0.008).Conclusion: It was attained ultimately that CD4 and CD8 levels could be a desirable diagnostic markers for Anthracosis and TB, respectively.
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Differential diagnosis of tuberculosis, sarcoidosis, and Anthracosis by CD8, CD3, and CD4 levels by flow-cytometry
2019Co-Authors: Nazanin Akbarifazli, Atefeh Abedini, Esmail MortazAbstract:Background: Determination of a desirable diagnostic tests is an issue of importance especially to differentiate between tuberculosis) TB(, sarcoidosis, and Anthracosis. The purpose of the present study was to determine the differential diagnosis of tuberculosis, sarcoidosis and Anthracosis with CD8, CD3, and CD4 by flow-cytometry. Materials and Methods: In this descriptive cross-sectional comparative survey, 40 consecutive patients attending to Masih Daneshvari Hospital in Tehran were enrolled and CD4/CD8 ratio, CD8, CD3, and CD4 were determined by flow-cytometry and compared across patients with three diseases including tuberculosis, sarcoidosis, and Anthracosis.Results: The results demonstrated that CD4 was significantly higher in Anthracosis cases (P
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Determination of CD4, CD8, and IL-8 levels in serum and bronchoalveolar lavage fluid of Anthracosis patients
Biomedical and Biotechnology Research Journal (BBRJ), 2018Co-Authors: Arda Kiani, Mehdi Ramazanpour, Fatemeh Razavi, Hamidreza Jamaati, Esmaeil Mortaz, Atefeh AbediniAbstract:Background: Anthracosis is a chronic pulmonary disease with black pigmentation of the bronchial mucosa, caused by carbon accumulation in the lungs, and in some cases, associated with pulmonary tuberculosis (TB). The purpose of this study is to evaluate the serum and bronchoalveolar lavage (BAL) fluid levels of the CD4/CD8 ratio and interleukin 8 (IL-8), as the potential diagnosis and prognosis biomarkers for Anthracosis. Methods: Of 60 patients, referred to the Masih Daneshvari Hospital, 30 Anthracosis patients, confirmed by bronchoscopy, were included as the case group. BAL sample and blood samples were collected from all individuals and sent to the immunology laboratory. Patients with no Anthracosis on bronchoscopy, and suspected to have TB, were included in the control group. In addition, BAL samples were used for BK-polymerase chain reaction. Results: In this case–control study, 30 Anthracosis patients and 30 controls were investigated. There were no significant differences in IL-8 of patients with Anthracosis, compared to others. There were significant differences of the CD4/CD8 ratio in BAL fluid, between Anthracosis cases and the control group (1.01 ± 0.77 vs. 2.41 ± 3.50; P = 0.04), and significant differences were seen in serum levels of two groups (P = 0.02). The rate of the confirmed pulmonary TB which was 88.9% in patients with Anthracosis was significantly higher than the control group. Conclusion: These outcomes suggest that changes in the CD4/C8 levels in serum and BAL fluid may have a leading role in the diagnosis or prognosis of Anthracosis. In addition, due to the strong association of Anthracosis and pulmonary TB, TB should be considered in patients with Anthracosis, which in turn can eventuate to the early distinguish and cure of the patients.
Karen L. Reckamp - One of the best experts on this subject based on the ideXlab platform.
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myeloid clusters are associated with a pro metastatic environment and poor prognosis in smoking related early stage non small cell lung cancer
PLOS ONE, 2013Co-Authors: Wang Zhang, Chunmei Yang, Sachin Allahabadi, Shaira Bhanji, Robert A. Figlin, Hua Yu, Karen L. ReckampAbstract:Background: This study aimed to understand the role of myeloid cell clusters in uninvolved regional lymph nodes from early stage non-small cell lung cancer patients. Methods: Uninvolved regional lymph node sections from 67 patients with stage I–III resected non-small cell lung cancer were immunostained to detect myeloid clusters, STAT3 activity and occult metastasis. Anthracosis intensity, myeloid cluster infiltration associated with Anthracosis and pSTAT3 level were scored and correlated with patient survival. Multivariate Cox regression analysis was performed with prognostic variables. Human macrophages were used for in vitro nicotine treatment. Results: \(CD68^+\) myeloid clusters associated with Anthracosis and with an immunosuppressive and metastasis-promoting phenotype and elevated overall STAT3 activity were observed in uninvolved lymph nodes. In patients with a smoking history, myeloid cluster score significantly correlated with Anthracosis intensity and pSTAT3 level (P
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Positive correlations between myeloid clusters and STAT3 activity in patients with a smoking history.
2013Co-Authors: Wang Zhang, Sumanta K. Pal, Xueli Liu, Chunmei Yang, Sachin Allahabadi, Shaira Bhanji, Robert A. Figlin, Karen L. ReckampAbstract:(A) In patients with a smoking history, but not in those without a history of smoking, myeloid cluster infiltration associated with Anthracosis correlates with Anthracosis intensity and overall STAT3 activity; Anthracosis correlates with STAT3 activity in myeloid clusters associated with Anthracosis. The correlation was determined with nonparametric Spearman's rank correlation test. Shown are means ± SEM, ** P
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Myeloid clusters and pSTAT3 predicts survival in NSCLC patients with a smoking history.
2013Co-Authors: Wang Zhang, Sumanta K. Pal, Xueli Liu, Chunmei Yang, Sachin Allahabadi, Shaira Bhanji, Robert A. Figlin, Karen L. ReckampAbstract:Kaplan-Meier curves showing the survival of patients grouped by: (A) myeloid cluster infiltration associated with Anthracosis, (B) overall pSTAT3 level, (C) the combination of nodal stage and myeloid clusters associated with Anthracosis, (D) the combination of myeloid clusters associated with Anthracosis and overall pSTAT3 level (n = 49).
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Myeloid clusters in uninvolved LNs colocalize with occult metastasis and cFn.
2013Co-Authors: Wang Zhang, Sumanta K. Pal, Xueli Liu, Chunmei Yang, Sachin Allahabadi, Shaira Bhanji, Robert A. Figlin, Karen L. ReckampAbstract:(A) IHC staining showing occult metastasis in “uninvolved” LNs (top two panels) and metastatic tumor cells in an involved LN as the positive control (lower left panel) (Scale bar, 50 µm). The bar graph shows the correlation between myeloid cluster infiltration associated with Anthracosis and the presence of occult metastasis (OM). The P value was determined by nonparametric Spearman's rank correlation test. (B) Confocal images showing the colocalization of pan-CK+ NSCLC cells (arrows) and CD68+ myeloid clusters associated with Anthracosis. (Scale bar, 50 µm) (C) Confocal images showing the colocalization of myeloid clusters associated with Anthracosis (arrows) and cFn. (Scale bar, 50 µm)
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Tumor-promoting phenotype of the myeloid clusters.
2013Co-Authors: Wang Zhang, Sumanta K. Pal, Xueli Liu, Chunmei Yang, Sachin Allahabadi, Shaira Bhanji, Robert A. Figlin, Karen L. ReckampAbstract:IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with Anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with Anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P
Carmen Diva Saldiva De André - One of the best experts on this subject based on the ideXlab platform.
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Levels of Polonium-210 in brain and pulmonary tissues: Preliminary study in autopsies conducted in the city of Sao Paulo, Brazil.
Scientific reports, 2020Co-Authors: Nathalia Villa Dos Santos, Lisie Tocci Justo, Carmen Diva Saldiva De André, Carolina L.z. Vieira, Paulo Hilário Nascimento Saldiva, Barbara Paci Mazzilli, Mitiko Saiki, Catia H.r. Saueia, Marcelo Bessa Nisti, Petros KoutrakisAbstract:The accumulation of detectable amounts of radon progeny in human tissues may be a risk factor for development and progression of chronic diseases. In this preliminary study, we analyzed the levels of alpha-emitting radon progeny Polonium-210 (210Po) in the olfactory epithelium, olfactory bulb, frontal lobe, and lung tissues in cadavers from the city of Sao Paulo, SP, Brazil. We also assessed the association between 210Po levels and exposure parameters for urban air pollution using linear regression models adjusted for age, sex, smoke, time living in Sao Paulo, daily commuting, socioeconomic index, and Anthracosis (traffic-related black carbon accumulation in the pleural region and in lymph). Our findings show that the concentration of 210Po was associated with Anthracosis in lungs of non-smokers (coefficient = 6.0; standard error = 2.9; p = 0.04). Individuals with lower socioeconomic status also had significantly higher 210Po levels in lungs (coefficient = −1.19; standard error = 0.58; p = 0.042). The olfactory bulb had higher 210Po levels than either olfactory epithelium (p = 0.071), frontal lobe (p
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Pleural Anthracosis as an indicator of lifetime exposure to urban air pollution: An autopsy-based study in Sao Paulo
Environmental research, 2019Co-Authors: Ana Paula Cremasco Takano, Lisie Tocci Justo, Nathalia Villa Dos Santos, Mônica V. Marquezini, Paulo Afonso De André, Francisco Marcelo Monteiro Da Rocha, Carlos Augusto Pasqualucci, Ligia Vizeu Barrozo, Julio M. Singer, Carmen Diva Saldiva De AndréAbstract:Abstract Many studies have been conducted to evaluate the association between air pollution and adverse health effects using a wide variety of methods to assess exposure. However, the assessment of individual long-term exposure to ambient air pollution is a challenging task and has not been evaluated in a large autopsy study. Our goal was to investigate whether exposure to urban air pollution is associated to the degree of lung Anthracosis, considering modifying factors such as personal habits, mobility patterns and occupational activities. We conducted a study in Sao Paulo, Brazil from February 2017 to June 2018, combining epidemiological, spatial analysis and autopsy-based approaches. Information about residential address, socio-demographic details, occupation, smoking status, time of residence in the city and time spent commuting was collected via questionnaires applied to the next-of-kin. Images of the pleura surface from upper and lower lobes were used to quantify Anthracosis in the lungs. We used multiple regression models to assess the association between the amount of carbon deposits in human lungs, measured by the fraction of pleural Anthracosis (FA), and potential explanatory variables. We analyzed 413 cases and our data showed that for each additional hour spent in daily commuting, the ratio FA/(1-FA) is multiplied by 1.05 (95% confidence interval: [1.02; 1.08]). The estimated coefficient for daily hours spent in traffic was not considerably affected by the inclusion of socio-demographic variables and smoking habits. We estimate a tobacco equivalent dose of 5 cigarettes per day in a city where annual PM2.5 concentration oscillates around 25 μg/m3. Pleural Anthracosis is a potential index of lifetime exposure to traffic-derived air pollution.