The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Kazuhiko Ishihara - One of the best experts on this subject based on the ideXlab platform.
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Cytocompatible and multifunctional polymeric nanoparticles for transportation of bioactive molecules into and within Cells
Science and Technology of Advanced Materials, 2016Co-Authors: Kazuhiko Ishihara, Yoshihiro Tsukamoto, Weixin Chen, Yihua Liu, Yuuki InoueAbstract:© 2016 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis. Multifunctional polymeric nanoparticles are materials with great potential for a wide range of biomedical applications. For progression in this area of research, unfavorable interactions of these nanoparticles with proteins and Cells must be avoided in biological environments, for example, through treatment of the nanoparticle surfaces. Construction of an Artificial Cell membrane structure based on polymers bearing the zwitterionic phosphorylcholine group can prevent biological reactions at the surface effectively. In addition, certain bioactive molecules can be immobilized on the surface of the polymer to generate enough affinity to capture target biomolecules. Furthermore, entrapment of inorganic nanoparticles inside polymeric matrices enhances the nanoparticle functionality significantly. This review summarizes the preparation and characterization of cytocompatible and multifunctional polymeric nanoparticles; it analyzes the efficiency of their fluorescence function, the nature of the Artificial Cell membrane structure, and their performance as in-Cell devices; and finally, it evaluates both their chemical reactivity and effects in Cells.
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integrated functional nanocolloids covered with Artificial Cell membranes for biomedical applications
Nano Today, 2011Co-Authors: Ryosuke Matsuno, Kazuhiko IshiharaAbstract:Summary The functionality of nanocolloids used in biomedical applications are subject to strong interference arising from significant interactions with biological components such as proteins and Cells. Among the known examples of surface treatment of nanocolloids, the construction of an Artificial Cell membrane structure based on phospholipid polymers has proven effective in preventing the occurrence of biological reactions at the surface. Furthermore, certain bioactive molecules can be immobilized on the surface of the phospholipid polymer platform to generate bioaffinity for other biomolecules. This review describes preparation and characterization of integrated functional nanocolloids covered by Artificial Cell membrane structures and their performance in biomedical applications.
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Integrated functional nanocolloids covered with Artificial Cell membranes for biomedical applications
Nano Today, 2011Co-Authors: Ryuichi Matsuno, Kazuhiko IshiharaAbstract:The functionality of nanocolloids used in biomedical applications are subject to strong interference arising from significant interactions with biological components such as proteins and Cells. Among the known examples of surface treatment of nanocolloids, the construction of an Artificial Cell membrane structure based on phospholipid polymers has proven effective in preventing the occurrence of biological reactions at the surface. Furthermore, certain bioactive molecules can be immobilized on the surface of the phospholipid polymer platform to generate bioaffinity for other biomolecules. This review describes preparation and characterization of integrated functional nanocolloids covered by Artificial Cell membrane structures and their performance in biomedical applications. © 2010 Elsevier Ltd.
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antibody immobilization to phospholipid polymer layer on gold substrate of quartz crystal microbalance immunosensor
Colloids and Surfaces B: Biointerfaces, 2007Co-Authors: Shigeru Kurosawa, Jongwon Park, Madoka Takai, Kazuhiko IshiharaAbstract:Abstract To modify gold electrode for immunosensor to construct an Artificial Cell membrane structure, water-soluble amphiphilic phospholipid polymer, poly[2-methacryloyloxyehtyl phosphorylcholine- co - n -butyl methacrylate- co - p -nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate (PMBN)] was applied. The polymer had active ester groups for immobilization of biomolecules and it was converted partially to thiol groups for binding to gold substrates. The partially thiolated PMBN was adsorbed on a gold electrode of quartz crystal microbalance (QCM). Surface characterization of adsorbed PMBN layers was thoroughly investigated with reflectance anisotropy spectroscopy, ellipsometry spectroscopy, dynamic contact angle and X-ray photoelectron spectroscopy measurements. Among several PMBN, having different degree of thiolation, it was concluded that 21.5% thiolated PMBN layer had the most well-ordered phosphorylcholine groups in its outer surface. The proteins adsorption test revealed that the phosphorylcholine group on the outer side of PMBN layers, which was substituted their active ester groups by glycine, showed suppress the non-specific adsorption of proteins, such as bovine serum albumin and γ-globulin. Also, through antigen–antibody binding evaluation, the anti-C-reactive protein antibody immobilized on the PMBN surface worked well and it was confirmed that denaturation of the antibody on the PMBN layers was hardly occurred in spite of 60 days storage at 4 °C. The antibody conjugated phospholipid polymer layer with well-ordered phosphorylcholine group could be outstanding functional membrane for biomedical diagnostic devices without non-specific binding and reduction of immunologic activity of immobilized antibody.
Thomas Ming Swi Chang - One of the best experts on this subject based on the ideXlab platform.
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Artificial Cell evolves into nanomedicine biotherapeutics blood substitutes drug delivery enzyme gene therapy cancer therapy Cell stem Cell therapy nanoparticles liposomes bioencapsulation replicating synthetic Cells Cell encapsulation scaffold bioso
Artificial Cells Nanomedicine and Biotechnology, 2019Co-Authors: Thomas Ming Swi ChangAbstract:AbstractIt is only in the last 20 years that many of the original ideas on Artificial Cells are being increasingly applied and extended by researchers around the world. Artificial Cell has now evolved into nanomedicine, biotherapeutics, blood substitutes, drug delivery, enzyme/gene therapy, cancer therapy, Cell/stem Cell therapy, nanoparticles, liposomes, bioencapsulation, replicating synthetic Cells, Cell encapsulation/scaffold, biosorbent/immunosorbent haemoperfusion/plasmapheresis, regenerative medicine, encapsulated microbe, nanobiotechnology, nanotechnology and other areas. More futuristic research includes nanorobot, nanocomputer, multimodal locomotion delivery robot and others. This review starts with a general overview followed by specific examples in more details.
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present status of modified hemoglobin as blood substitutes and oral therapy for end stage renal failure using Artificial Cells containing genetically engineered Cells
Annals of the New York Academy of Sciences, 2006Co-Authors: Thomas Ming Swi ChangAbstract:: Artificial Cell or bioencapsulation has been developed for use in bioArtificial organs, drug delivery, blood substitutes, and other areas. Recent rapid advances in modified hemoglobin blood substitutes have resulted in advance stages of Phase III clinical trials. Another area of use is in oral therapy, using Artificial Cells microencapsulated with genetically engineered Cells for use in end stage renal failure and other conditions.
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Artificial Cells for Blood Substitutes, Enzyme Therapy, Cell Therapy and Drug Delivery
Applications of Cell Immobilisation Biotechnology, 2005Co-Authors: Thomas Ming Swi ChangAbstract:Artificial Cells are being actively investigated for medical and biotechnological applications. The earliest routine clinical use of Artificial Cells is in the form of coated activated charcoal for haemoperfusion. Implantation of encapsulated Cells are being studied for the treatment of diabetes, liver failure and the use of encapsulated genetically engineered Cells for gene therapy. Blood substitutes based on modified haemoglobin are already in Phase III clinical trials in patients with as much as 20 units infused into each patient during trauma surgery. Artificial Cells containing enzymes are being developed for clinical trial in hereditary enzyme deficiency diseases and other diseases. Artificial Cell is also being investigated for drug delivery and for other uses in biotechnology, chemical engineering and medicine.
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Artificial Cells for Cell and Organ Replacements
Artificial Organs, 2004Co-Authors: Thomas Ming Swi ChangAbstract:: The Artificial Cell is a Canadian invention (Chang, Science, 1964). This principle is being actively investigated for use in Cell and organ replacements. The earliest routine clinical use of Artificial Cells is in the form of coated activated charcoal for hemoperfusion for use in the removal of drugs, and toxins and waste in uremia and liver failure. Encapsulated Cells are being studied for the treatment of diabetes, liver failure, and kidney failure, and the use of encapsulated genetically-engineered Cells is being investigated for gene therapy. Blood substitutes based on modified hemoglobin are already in Phase III clinical trials in patients, with as much as 20 units being infused into each patient during trauma surgery. Artificial Cells containing enzymes are being developed for clinical trial in hereditary enzyme deficiency diseases and other diseases. The Artificial Cell is also being investigated for drug delivery and for other uses in biotechnology, chemical engineering, and medicine.
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Artificial Cells for replacement of metabolic organ functions.
Artificial Cells Blood Substitutes and Biotechnology, 2003Co-Authors: Thomas Ming Swi ChangAbstract:Artificial Cells are being actively investigated for use in the replacement of Cell and organ functions, especially related to metabolic functions. The earliest routine clinical use of Artificial Cells is in the form of coated activated charcoal for hemoperfusion. Implantation of encapsulated Cells are being studied for the treatment of diabetes, liver failure, kidney failure and the use of encapsulated genetically engineered Cells for gene therapy. Blood substitutes based on modified hemoglobin are already in Phase III clinical trials in patients with as much as 20 units infused into each patient during trauma surgery. Artificial Cells containing enzymes are being developed for clinical trial in hereditary enzyme deficiency diseases and other diseases. Artificial Cell is also being investigated for drug delivery and for other uses in biotechnology, chemical engineering and medicine.
Ryuichi Matsuno - One of the best experts on this subject based on the ideXlab platform.
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Integrated functional nanocolloids covered with Artificial Cell membranes for biomedical applications
Nano Today, 2011Co-Authors: Ryuichi Matsuno, Kazuhiko IshiharaAbstract:The functionality of nanocolloids used in biomedical applications are subject to strong interference arising from significant interactions with biological components such as proteins and Cells. Among the known examples of surface treatment of nanocolloids, the construction of an Artificial Cell membrane structure based on phospholipid polymers has proven effective in preventing the occurrence of biological reactions at the surface. Furthermore, certain bioactive molecules can be immobilized on the surface of the phospholipid polymer platform to generate bioaffinity for other biomolecules. This review describes preparation and characterization of integrated functional nanocolloids covered by Artificial Cell membrane structures and their performance in biomedical applications. © 2010 Elsevier Ltd.
Ryosuke Matsuno - One of the best experts on this subject based on the ideXlab platform.
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integrated functional nanocolloids covered with Artificial Cell membranes for biomedical applications
Nano Today, 2011Co-Authors: Ryosuke Matsuno, Kazuhiko IshiharaAbstract:Summary The functionality of nanocolloids used in biomedical applications are subject to strong interference arising from significant interactions with biological components such as proteins and Cells. Among the known examples of surface treatment of nanocolloids, the construction of an Artificial Cell membrane structure based on phospholipid polymers has proven effective in preventing the occurrence of biological reactions at the surface. Furthermore, certain bioactive molecules can be immobilized on the surface of the phospholipid polymer platform to generate bioaffinity for other biomolecules. This review describes preparation and characterization of integrated functional nanocolloids covered by Artificial Cell membrane structures and their performance in biomedical applications.
Kai Ostermann - One of the best experts on this subject based on the ideXlab platform.
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Artificial Cell Cell communication as an emerging tool in synthetic biology applications
Journal of Biological Engineering, 2015Co-Authors: Stefan Hennig, Gerhard Rodel, Kai OstermannAbstract:Cell-Cell communication is a widespread phenomenon in nature, ranging from bacterial quorum sensing and fungal pheromone communication to Cellular crosstalk in multiCellular eukaryotes. These communication modes offer the possibility to control the behavior of an entire community by modifying the performance of individual Cells in specific ways. Synthetic biology, i.e., the implementation of Artificial functions within biological systems, is a promising approach towards the engineering of sophisticated, autonomous devices based on specifically functionalized Cells. With the growing complexity of the functions performed by such systems, both the risk of circuit crosstalk and the metabolic burden resulting from the expression of numerous foreign genes are increasing. Therefore, systems based on a single type of Cells are no longer feasible. Synthetic biology approaches with multiple subpopulations of specifically functionalized Cells, wired by Artificial Cell-Cell communication systems, provide an attractive and powerful alternative. Here we review recent applications of synthetic Cell-Cell communication systems with a specific focus on recent advances with fungal hosts.
