The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Jos Malda - One of the best experts on this subject based on the ideXlab platform.
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multitechnology Biofabrication a new approach for the manufacturing of functional tissue structures
Trends in Biotechnology, 2020Co-Authors: Gordon G. Wallace, Miguel Castilho, Mylene De Ruijter, Stephen Beirne, Claire C Villette, Keita Ito, Jos MaldaAbstract:Most available 3D Biofabrication technologies rely on single-component deposition methods, such as inkjet, extrusion, or light-assisted printing. It is unlikely that any of these technologies used individually would be able to replicate the complexity and functionality of living tissues. Recently, new Biofabrication approaches have emerged that integrate multiple manufacturing technologies into a single Biofabrication platform. This has led to fabricated structures with improved functionality. In this review, we provide a comprehensive overview of recent advances in the integration of different manufacturing technologies with the aim to fabricate more functional tissue structures. We provide our vision on the future of additive manufacturing (AM) technology, digital design, and the use of artificial intelligence (AI) in the field of Biofabrication.
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a definition of bioinks and their distinction from biomaterial inks
Biofabrication, 2018Co-Authors: Jürgen Groll, Tomasz Jungst, Vladimir Mironov, Jos Malda, Jason A Burdick, Brian Derby, Michael Gelinsky, Sarah C Heilshorn, K Nakayama, Aleksandr OvsianikovAbstract:Biofabrication aims to fabricate biologically functional products through bioprinting or bioassembly (Groll et al 2016 Biofabrication 8 013001). In Biofabrication processes, cells are positioned at defined coordinates in three-dimensional space using automated and computer controlled techniques (Moroni et al 2018 Trends Biotechnol. 36 384-402), usually with the aid of biomaterials that are either (i) directly processed with the cells as suspensions/dispersions, (ii) deposited simultaneously in a separate printing process, or (iii) used as a transient support material. Materials that are suited for Biofabrication are often referred to as bioinks and have become an important area of research within the field. In view of this special issue on bioinks, we aim herein to briefly summarize the historic evolution of this term within the field of Biofabrication. Furthermore, we propose a simple but general definition of bioinks, and clarify its distinction from biomaterial inks.
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Bio-resin for high resolution lithography-based Biofabrication of complex cell-laden constructs
Biofabrication, 2018Co-Authors: Khoon S. Lim, Miguel D. Castilho, Cesar R. Alcala-orozco, Kim M.a. Van Dorenmalen, Gary J. Hooper, Riccardo Levato, Ferry Petrus Wilhelmus Melchels, Debby Gawlitta, Pedro F. Costa, Jos MaldaAbstract:Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bio-resins and their application in lithography-based Biofabrication, limiting the advancement of this promising technology. In this study, we developed a new bio-resin based on methacrylated poly(vinyl alcohol) (PVA-MA), gelatin-methacryloyl (Gel-MA) and a transition metal-based visible light photoinitiator. The utilization of a visible light photo-initiating system displaying high molar absorptivity allowed the bioprinting of constructs with high resolution features, in the range of 25–50 μm. Biofunctionalization of the resin with 1 wt% Gel-MA allowed long term survival (>90%) of encapsulated cells up to 21 d, and enabled attachment and spreading of endothelial cells seeded on the printed hydrogels. Cell-laden hydrogel constructs of high resolution with complex and ordered architecture were successfully bioprinted, where the encapsulated cells remained viable, homogenously distributed and functional. Bone and cartilage tissue synthesis was confirmed by encapsulated stem cells, underlining the potential of these DLP-bioprinted hydrogels for tissue engineering and Biofabrication. Overall, the PVA-MA/Gel-MA bio-resin is a promising material for Biofabrication and provides important cues for the further development of lithography-based bioprinting of complex, free-form living tissue analogues.
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Biofabrication a guide to technology and terminology
Trends in Biotechnology, 2017Co-Authors: Lorenzo Moroni, Vladimir Mironov, Jos Malda, Thomas Boland, Jason A Burdick, Brian Derby, Gabor Forgacs, Carmelo De Maria, Juergen Groll, Carlos MotaAbstract:Biofabrication holds the potential to generate constructs that more closely recapitulate the complexity and heterogeneity of tissues and organs than do currently available regenerative medicine therapies. Such constructs can be applied for tissue regeneration or as in vitro 3D models. Biofabrication is maturing and growing, and scientists with different backgrounds are joining this field, underscoring the need for unity regarding the use of terminology. We therefore believe that there is a compelling need to clarify the relationship between the different concepts, technologies, and descriptions of Biofabrication that are often used interchangeably or inconsistently in the current literature. Our objective is to provide a guide to the terminology for different technologies in the field which may serve as a reference for the Biofabrication community.
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converging Biofabrication and organoid technologies the next frontier in hepatic and intestinal tissue engineering
Biofabrication, 2017Co-Authors: Kerstin Schneeberger, Pedro F. Costa, Bart Spee, Norman Sachs, Hans Clevers, Jos MaldaAbstract:Adult tissue stem cells can form self-organizing 3D organoids in vitro. Organoids resemble small units of their organ of origin and have great potential for tissue engineering, as well as models of disease. However, current culture technology limits the size, architecture and complexity of organoids. Here, we review the establishment of intestinal and hepatic organoids and discuss how the convergence of organoids and Biofabrication technologies can help overcome current limitations, and thereby further advance the translational application of organoids in tissue engineering and regenerative medicine.
Gregory F. Payne - One of the best experts on this subject based on the ideXlab platform.
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Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
Biomaterials, 2012Co-Authors: Xiaolong Luo, Gregory F. Payne, Gary W. Rubloff, Chenyu Tsao, Yi Cheng, Jordan Betz, William E. BentleyAbstract:Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we present “Biofabrication” in microfluidics as one approach that enables in-situ organization of living cells in microenvironments with spatiotemporal control and programmability. We construct bacterial biofilm mimics that offer detailed understanding and subsequent control of population-based quorum sensing (QS) behaviors in a manner decoupled from cell number. Our approach reveals signaling patterns among bacterial cells within a single biofilm as well as behaviors that are coordinated between two communicating biofilms. We envision versatile use of this Biofabrication strategy for cell–cell interaction studies and small molecule drug discovery.
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Biofabrication to build the biology device interface
Biofabrication, 2010Co-Authors: Yi Liu, Gregory F. Payne, James N. Culver, Gary W. Rubloff, William E. Bentley, Reza Ghodssi, Eunkyoung KimAbstract:The last century witnessed spectacular advances in both microelectronics and biotechnology yet there was little synergy between the two. A challenge to their integration is that biological and electronic systems are constructed using divergent fabrication paradigms. Biology fabricates bottom-up with labile components, while microelectronic devices are fabricated top-down using methods that are 'bio-incompatible'. Biofabrication--the use of biological materials and mechanisms for construction--offers the opportunity to span these fabrication paradigms by providing convergent approaches for building the bio-device interface. Integral to Biofabrication are stimuli-responsive materials (e.g. film-forming polysaccharides) that allow directed assembly under near physiological conditions in response to device-imposed signals. Biomolecular engineering, through recombinant technology, allows biological components to be endowed with information for assembly (e.g. encoded in a protein's amino acid sequence). Finally, self-assembly and enzymatic assembly provide the mechanisms for construction over a hierarchy of length scales. Here, we review recent advances in the use of Biofabrication to build the bio-device interface. We anticipate that the Biofabrication toolbox will expand over the next decade as more researchers enlist the unique construction capabilities of biology. Further, we look forward to observing the application of this toolbox to create devices that can better diagnose disease, detect pathogens and discover drugs. Finally, we expect that Biofabrication will enable the effective interfacing of biology with electronics to create implantable devices for personalized and regenerative medicine.
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in situ generation of ph gradients in microfluidic devices for Biofabrication of freestanding semi permeable chitosan membranes
Lab on a Chip, 2010Co-Authors: Xiaolong Luo, Gregory F. Payne, William E. Bentley, Jordan Betz, Dean Berlin, Gary W. RubloffAbstract:We report the in situ generation of pH gradients in microfluidic devices for Biofabrication of freestanding, semi-permeable chitosan membranes. The pH-stimuli-responsive polysaccharide chitosan was enlisted to form a freestanding hydrophilic membrane structure in microfluidic networks where pH gradients are generated at the converging interface between a slightly acidic chitosan solution and a slightly basic buffer solution. A simple and effective pumping strategy was devised to realize a stable flow interface thereby generating a stable, well-controlled and localized pH gradient. Chitosan molecules were deprotonated at the flow interface, causing gelation and solidification of a freestanding chitosan membrane from a nucleation point at the junction of two converging flow streams to an anchoring point where the two flow streams diverge to two output channels. The fabricated chitosan membranes were about 30–60 µm thick and uniform throughout the flow interface inside the microchannels. A T-shaped membrane formed by sequentially fabricating orthogonal membranes demonstrates flexibility of the assembly process. The membranes are permeable to aqueous solutions and are removed by mildly acidic solutions. Permeability tests suggested that the membrane pore size was a few nanometres, i.e., the size range of antibodies. Building on the widely reported use of chitosan as a soft interconnect for biological components and microfabricated devices and the broad applications of membrane functionalities in microsystems, we believe that the facile, rapid Biofabrication of freestanding chitosan membranes can be applied to many biochemical, bioanalytical, biosensing applications and cellular studies.
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Biofabrication with chitosan
Biomacromolecules, 2005Co-Authors: Hyunmin Yi, Li Qun Wu, James N. Culver, Gary W. Rubloff, William E. Bentley, Reza Ghodssi, Gregory F. PayneAbstract:The traditional motivation for integrating biological components into microfabricated devices has been to create biosensors that meld the molecular recognition capabilities of biology with the signal processing capabilities of electronic devices. However, a different motivation is emerging; biological components are being explored to radically change how fabrication is achieved at the micro- and nanoscales. Here we review Biofabrication, the use of biological materials for fabrication, and focus on three specific Biofabrication approaches: directed assembly, where localized external stimuli are employed to guide assembly; enzymatic assembly, where selective biocatalysts are enlisted to build macromolecular structure; and self-assembly, where information internal to the biological material guides its own assembly. Also reviewed are recent results with the aminopolysaccharide chitosan, a material that offers a combination of properties uniquely suited for Biofabrication. In particular, chitosan can be directed to assemble in response to locally applied electrical signals, and the chitosan backbone provides sites that can be employed for the assembly of proteins, nucleic acids, and virus particles.
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Biofabrication using biological materials and biocatalysts to construct nanostructured assemblies
Trends in Biotechnology, 2004Co-Authors: Gregory F. PayneAbstract:Emerging opportunities are placing greater demands on device fabrication: next-generation microelectronics will need minimum features of less than 100 nm, high-throughput drug screening will require facile methods to incorporate sensitive biological components into microelectromechanical systems (MEMS), and implantable devices will need to be built from biocompatible materials. Increasingly, these emerging demands are being addressed by combining traditional microfabrication methods with 'Biofabrication': namely, the use of biologically derived materials and biocatalysts. Recent fabrication techniques are using biological construction materials as process aids or structural components, and enzymes are being considered for their potential to fabricate devices with high selectivity under mild conditions. If incompatibilities between biology and microfabrication can be eliminated, then Biofabrication will be poised to emerge as the standard for nanoscale construction.
Jürgen Groll - One of the best experts on this subject based on the ideXlab platform.
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a definition of bioinks and their distinction from biomaterial inks
Biofabrication, 2018Co-Authors: Jürgen Groll, Tomasz Jungst, Vladimir Mironov, Jos Malda, Jason A Burdick, Brian Derby, Michael Gelinsky, Sarah C Heilshorn, K Nakayama, Aleksandr OvsianikovAbstract:Biofabrication aims to fabricate biologically functional products through bioprinting or bioassembly (Groll et al 2016 Biofabrication 8 013001). In Biofabrication processes, cells are positioned at defined coordinates in three-dimensional space using automated and computer controlled techniques (Moroni et al 2018 Trends Biotechnol. 36 384-402), usually with the aid of biomaterials that are either (i) directly processed with the cells as suspensions/dispersions, (ii) deposited simultaneously in a separate printing process, or (iii) used as a transient support material. Materials that are suited for Biofabrication are often referred to as bioinks and have become an important area of research within the field. In view of this special issue on bioinks, we aim herein to briefly summarize the historic evolution of this term within the field of Biofabrication. Furthermore, we propose a simple but general definition of bioinks, and clarify its distinction from biomaterial inks.
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Biofabrication new approaches for tissue regeneration
Handchirurgie Mikrochirurgie Plastische Chirurgie, 2018Co-Authors: Raymund E Horch, Jürgen Groll, Aldo R Boccaccini, Annika Weigand, Harald Wajant, Andreas ArkudasAbstract:Hintergrund Das Aufkommen von Tissue Engineering (TE) in den fruhen 1990er Jahren wurde durch den zunehmenden Bedarf an funktionellem Gewebe und Organersatz gefordert. Das klassische TE basiert dabei auf der Kombination von Tragermatrizen, Zellen und Wachstumsfaktoren, um verlorenes oder beschadigtes Gewebe und Organe wieder herzustellen. Trotz beachtlicher Ergebnisse in vitro und in experimentellen Ansatzen hat der Mangel an fruher Vaskularisierung eine Umsetzung in die tagliche klinische Praxis bisher behindert Ein neues Forschungsfeld mit dem Namen „Biofabrikation“,. das die neuesten 3D-Drucktechnologien nutzt, zielt darauf ab, verschiedene Zellen, Biomaterialien und Molekule hierarchisch und raumlich in eine Matrix zu integrieren, um eine gerichtete Reifung von kunstlichem Gewebe zu gewahrleisten. Material und Methoden Eine Literaturrecherche der relevanten Publikationen zum Thema Biofabrikation und Bioprinting wurde mit der PubMed-Datenbank durchgefuhrt. Relevante Papiere wurden ausgewahlt und mit einer sekundaren Analyse von spezifischen Zitaten uber die Bioprinting-Techniken bewertet. Ergebnisse Es wurden 180 relevante Publikationen mit den oben genannten Schlusselwortern identifiziert und ausgewertet. Grundprinzipien in dem Entwicklungsfeld der Biodrucktechnologie konnten herausgefiltert werden. Die Schlusselelemente umfassen die Hochdurchsatzanordnung von Zellen und die Herstellung von komplexen und funktionellen, hierarchisch organisierten Gewebekonstrukten. Es wurden funf relevante technologische Prinzipien fur das Bioprinting identifiziert, wie Stereolithographie, Extrusionsbasiertes Drucken, laserunterstutztes Drucken, Inkjet-basiertes Drucken und Nano-Bioprinting. Die verschiedenen technischen Methoden des 3D-Drucks wurden mit verschiedenen positiven, aber auch negativen Auswirkungen auf Zellen und Proteine wahrend des Druckprozesses assoziiert. Die Forschungsanstrengungen in diesem Bereich zielen offensichtlich auf die Entwicklung von optimierten so genannten Biotinten und verbesserten Drucktechnologien ab. Schlusfolgerung Diese Ubersicht beschreibt die Entwicklung der klassischen Methoden des TE in der Regenerativen Medizin in das sich rapide entwickelnde Gebiet der Biofabrikation durch Bioprinting. Die Vorteile des 3D-Bioprintings gegenuber herkommlichen Tissue Engineering-Techniken basieren auf der Anordnung von Zellen, Biomaterialien und Biomolekulen in einer raumlich kontrollierten Weise zur Reproduktion von nativen Gewebemakro-, Mikro- und Nanoarchitekturen, die nicht nur dazu genutzt werden konnen, funktionelle Ersatzgewebe oder Organe zu produzieren, sondern auch als neue Modelle fur die Grundlagenforschung dienen. Die Nachahmung der stromalen Mikroumgebung von Tumorzellen zur Untersuchung der Tumorbildung und -progression, der Metastasierung, Angiogenese und Modulation der damit verbundenen assoziierten Prozesse ist eine dieser Anwendungen in der aktuellen Forschung. Zu diesem Zweck wird eine enge Zusammenarbeit von Fachleuten aus den Bereichen Ingenieurswesen, Biomaterialwissenschaft, Zellbiologie und rekonstruktive Mikrochirurgie notwendig sein, um zukunftige Strategien zu entwickeln, die die derzeitigen Einschrankungen des artifiziellen Gewebe-Ersatzes uberwinden konnen.
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thiol ene clickable poly glycidol hydrogels for Biofabrication
Annals of Biomedical Engineering, 2017Co-Authors: Simone Stichler, Tomasz Jungst, Torsten Blunk, Martha Schamel, Ilona Zilkowski, Matthias Kuhlmann, Thomas Bock, Jorg Tesmar, Jürgen GrollAbstract:In this study we introduce linear poly(glycidol) (PG), a structural analog of poly(ethylene glycol) bearing side chains at each repeating unit, as polymer basis for bioink development. We prepare allyl- and thiol-functional linear PG that can rapidly be polymerized to a three-dimensionally cross-linked hydrogel network via UV mediated thiol-ene click reaction. Influence of polymer concentration and UV irradiation on mechanical properties and swelling behavior was examined. Thiol-functional PG was synthesized in two structural variations, one containing ester groups that are susceptible to hydrolytic cleavage, and the other one ester-free and stable against hydrolysis. This allowed the preparation of degradable and non-degradable hydrogels. Cytocompatibility of the hydrogel was demonstrated by encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Rheological properties of the hydrogels were adjusted for dispense plotting by addition of high molecular weight hyaluronic acid. The optimized formulation enabled highly reproducible plotting of constructs composed of 20 layers with an overall height of 3.90 mm.
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Biofabrication reappraising the definition of an evolving field
Biofabrication, 2016Co-Authors: Jürgen Groll, Vladimir Mironov, Thomas Boland, Torsten Blunk, Jason A Burdick, Dongwoo Cho, Paul D Dalton, Brian Derby, Gabor Forgacs, Lorenzo MoroniAbstract:Biofabrication is an evolving research field that has recently received significant attention. In particular, the adoption of Biofabrication concepts within the field of Tissue Engineering and Regenerative Medicine has grown tremendously, and has been accompanied by a growing inconsistency in terminology. This article aims at clarifying the position of Biofabrication as a research field with a special focus on its relation to and application for Tissue Engineering and Regenerative Medicine. Within this context, we propose a refined working definition of Biofabrication, including Bioprinting and Bioassembly as complementary strategies within Biofabrication.
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Biofabrication of Cell-Loaded 3D Spider Silk Constructs**
Angewandte Chemie, 2015Co-Authors: Kristin Schacht, Tomasz Jungst, Matthias Schweinlin, Andrea Ewald, Jürgen Groll, Thomas ScheibelAbstract:Biofabrication is an emerging and rapidly expanding field of research in which additive manufacturing techniques in combination with cell printing are exploited to generate hierarchical tissue-like structures. Materials that combine printability with cytocompatibility, so called bioinks, are currently the biggest bottleneck. Since recombinant spider silk proteins are non-immunogenic, cytocompatible, and exhibit physical crosslinking, their potential as a new bioink system was evaluated. Cell-loaded spider silk constructs can be printed by robotic dispensing without the need for crosslinking additives or thickeners for mechanical stabilization. Cells are able to adhere and proliferate with good viability over at least one week in such spider silk scaffolds. Introduction of a cell-binding motif to the spider silk protein further enables fine-tuned control over cell–material interactions. Spider silk hydrogels are thus a highly attractive novel bioink for Biofabrication.
Vladimir Mironov - One of the best experts on this subject based on the ideXlab platform.
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a definition of bioinks and their distinction from biomaterial inks
Biofabrication, 2018Co-Authors: Jürgen Groll, Tomasz Jungst, Vladimir Mironov, Jos Malda, Jason A Burdick, Brian Derby, Michael Gelinsky, Sarah C Heilshorn, K Nakayama, Aleksandr OvsianikovAbstract:Biofabrication aims to fabricate biologically functional products through bioprinting or bioassembly (Groll et al 2016 Biofabrication 8 013001). In Biofabrication processes, cells are positioned at defined coordinates in three-dimensional space using automated and computer controlled techniques (Moroni et al 2018 Trends Biotechnol. 36 384-402), usually with the aid of biomaterials that are either (i) directly processed with the cells as suspensions/dispersions, (ii) deposited simultaneously in a separate printing process, or (iii) used as a transient support material. Materials that are suited for Biofabrication are often referred to as bioinks and have become an important area of research within the field. In view of this special issue on bioinks, we aim herein to briefly summarize the historic evolution of this term within the field of Biofabrication. Furthermore, we propose a simple but general definition of bioinks, and clarify its distinction from biomaterial inks.
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from nano to macro enabling nanotechnologies for human organ Biofabrication electrospun nanofibers and hybrid technique
International Journal of Advances in Medical Biotechnology - IJAMB, 2018Co-Authors: Rodrigo A Rezende, Vladimir Mironov, Marcos A Sabino, Janaina Dernowsek, Fabio De Albuquerque Vilalba, Jorge Vicente Lopes SilvaAbstract:This review proposes to present how materials at nanolevel scale can contribute to the development of three-dimensional (3D) structures, human tissues, and organs which have macrolevel organization. Specific nanomaterials such as nanofibers and nanoparticles are presented and discussed in their application for biofabricating 3D human tissues and organs. The concept of self-assembling magnetic tissue spheroids as an intermediate mesolevel structure between nano and macrolevel organization and building blocks for Biofabrication in dual scale level of complex 3D human tissues and organs is detached. The challenges and perspectives of employing nanomaterials and nanotechnological strategies in the Biofabrication were also traced.
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Biofabrication a guide to technology and terminology
Trends in Biotechnology, 2017Co-Authors: Lorenzo Moroni, Vladimir Mironov, Jos Malda, Thomas Boland, Jason A Burdick, Brian Derby, Gabor Forgacs, Carmelo De Maria, Juergen Groll, Carlos MotaAbstract:Biofabrication holds the potential to generate constructs that more closely recapitulate the complexity and heterogeneity of tissues and organs than do currently available regenerative medicine therapies. Such constructs can be applied for tissue regeneration or as in vitro 3D models. Biofabrication is maturing and growing, and scientists with different backgrounds are joining this field, underscoring the need for unity regarding the use of terminology. We therefore believe that there is a compelling need to clarify the relationship between the different concepts, technologies, and descriptions of Biofabrication that are often used interchangeably or inconsistently in the current literature. Our objective is to provide a guide to the terminology for different technologies in the field which may serve as a reference for the Biofabrication community.
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Biofabrication reappraising the definition of an evolving field
Biofabrication, 2016Co-Authors: Jürgen Groll, Vladimir Mironov, Thomas Boland, Torsten Blunk, Jason A Burdick, Dongwoo Cho, Paul D Dalton, Brian Derby, Gabor Forgacs, Lorenzo MoroniAbstract:Biofabrication is an evolving research field that has recently received significant attention. In particular, the adoption of Biofabrication concepts within the field of Tissue Engineering and Regenerative Medicine has grown tremendously, and has been accompanied by a growing inconsistency in terminology. This article aims at clarifying the position of Biofabrication as a research field with a special focus on its relation to and application for Tissue Engineering and Regenerative Medicine. Within this context, we propose a refined working definition of Biofabrication, including Bioprinting and Bioassembly as complementary strategies within Biofabrication.
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organ printing from bioprinter to organ Biofabrication line
Current Opinion in Biotechnology, 2011Co-Authors: Vladimir Mironov, Vladimir Kasyanov, R. MarkwaldAbstract:Organ printing, or the layer by layer additive robotic Biofabrication of functional three-dimensional tissue and organ constructs using self-assembling tissue spheroid building blocks, is a rapidly emerging technology that promises to transform tissue engineering into a commercially successful biomedical industry. It is increasingly obvious that similar well-established industries implement automated robotic systems on the path to commercial translation and economic success. The use of robotic bioprinters alone however is not sufficient for the development of large industrial scale organ Biofabrication. The design and development of a fully integrated organ Biofabrication line is imperative for the commercial translation of organ printing technology. This paper presents recent progress and challenges in the development of the essential components of an organ Biofabrication line.
Ferry Petrus Wilhelmus Melchels - One of the best experts on this subject based on the ideXlab platform.
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Bio-resin for high resolution lithography-based Biofabrication of complex cell-laden constructs
Biofabrication, 2018Co-Authors: Khoon S. Lim, Miguel D. Castilho, Cesar R. Alcala-orozco, Kim M.a. Van Dorenmalen, Gary J. Hooper, Riccardo Levato, Ferry Petrus Wilhelmus Melchels, Debby Gawlitta, Pedro F. Costa, Jos MaldaAbstract:Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bio-resins and their application in lithography-based Biofabrication, limiting the advancement of this promising technology. In this study, we developed a new bio-resin based on methacrylated poly(vinyl alcohol) (PVA-MA), gelatin-methacryloyl (Gel-MA) and a transition metal-based visible light photoinitiator. The utilization of a visible light photo-initiating system displaying high molar absorptivity allowed the bioprinting of constructs with high resolution features, in the range of 25–50 μm. Biofunctionalization of the resin with 1 wt% Gel-MA allowed long term survival (>90%) of encapsulated cells up to 21 d, and enabled attachment and spreading of endothelial cells seeded on the printed hydrogels. Cell-laden hydrogel constructs of high resolution with complex and ordered architecture were successfully bioprinted, where the encapsulated cells remained viable, homogenously distributed and functional. Bone and cartilage tissue synthesis was confirmed by encapsulated stem cells, underlining the potential of these DLP-bioprinted hydrogels for tissue engineering and Biofabrication. Overall, the PVA-MA/Gel-MA bio-resin is a promising material for Biofabrication and provides important cues for the further development of lithography-based bioprinting of complex, free-form living tissue analogues.
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gelatin methacryloyl hydrogels towards Biofabrication based tissue repair
Trends in Biotechnology, 2016Co-Authors: Barbara J Klotz, Ferry Petrus Wilhelmus Melchels, Debby Gawlitta, Jos Malda, Antoine J W P RosenbergAbstract:Research over the past decade on the cell-biomaterial interface has shifted to the third dimension. Besides mimicking the native extracellular environment by 3D cell culture, hydrogels offer the possibility to generate well-defined 3D biofabricated tissue analogs. In this context, gelatin-methacryloyl (gelMA) hydrogels have recently gained increased attention. This interest is sparked by the combination of the inherent bioactivity of gelatin and the physicochemical tailorability of photo-crosslinkable hydrogels. GelMA is a versatile matrix that can be used to engineer tissue analogs ranging from vasculature to cartilage and bone. Convergence of biological and Biofabrication approaches is necessary to progress from merely proving cell functionality or construct shape fidelity towards regenerating tissues. GelMA has a critical pioneering role in this process and could be used to accelerate the development of clinically relevant applications.
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auricular reconstruction using Biofabrication based tissue engineering strategies
Biofabrication, 2015Co-Authors: Iris A Otto, Ferry Petrus Wilhelmus Melchels, Xin Zhao, Mark A Randolph, Moshe Kon, Corstiaan C Breugem, Jos MaldaAbstract:Auricular malformations, which impose a significant social and psychological burden, are currently treated using ear prostheses, synthetic implants or autologous implants derived from rib cartilage. Advances in the field of regenerative medicine and Biofabrication provide the possibility to engineer functional cartilage with intricate architectures and complex shapes using patient-derived or donor cells. However, the development of a successful auricular cartilage implant still faces a number of challenges. These challenges include the generation of a functional biochemical matrix, the fabrication of a customized anatomical shape, and maintenance of that shape. Biofabrication technologies may have the potential to overcome these challenges due to their ability to reproducibly deposit multiple materials in complex geometries in a highly controllable manner. This topical review summarizes this potential of Biofabrication technologies for the generation of implants for auricular reconstruction. In particular, it aims to discuss how Biofabrication technologies, although still in pre-clinical phase, could overcome the challenges of generating and maintaining the desired auricular shapes. Finally, remaining bottlenecks and future directions are discussed.
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Biofabrication of multi material anatomically shaped tissue constructs
Biofabrication, 2013Co-Authors: Jetze Visser, Ferry Petrus Wilhelmus Melchels, Wouter J.a. Dhert, Jos Malda, Benjamin Peters, Thijs J Burger, Jelle BoomstraAbstract:Additive manufacturing in the field of regenerative medicine aims to fabricate organized tissue-equivalents. However, the control over shape and composition of biofabricated constructs is still a challenge and needs to be improved. The current research aims to improve shape, by converging a number of biocompatible, quality construction materials into a single three-dimensional fiber deposition process. To demonstrate this, several models of complex anatomically shaped constructs were fabricated by combined deposition of poly(vinyl alcohol), poly(e-caprolactone), gelatin methacrylamide/gellan gum and alginate hydrogel. Sacrificial components were co-deposited as temporary support for overhang geometries and were removed after fabrication by immersion in aqueous solutions. Embedding of chondrocytes in the gelatin methacrylamide/gellan component demonstrated that the fabrication and the sacrificing procedure did not affect cell viability. Further, it was shown that anatomically shaped constructs can be successfully fabricated, yielding advanced porous thermoplastic polymer scaffolds, layered porous hydrogel constructs, as well as reinforced cell-laden hydrogel structures. In conclusion, anatomically shaped tissue constructs of clinically relevant sizes can be generated when employing multiple building and sacrificial materials in a single Biofabrication session. The current techniques offer improved control over both internal and external construct architecture underscoring its potential to generate customized implants for human tissue regeneration.
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gelatin methacrylamide hydrogels as potential biomaterials for fabrication of tissue engineered cartilage constructs
Macromolecular Bioscience, 2013Co-Authors: Wouter Schuurman, Ferry Petrus Wilhelmus Melchels, Wouter J.a. Dhert, Peter A Levett, Michiel W Pot, Paul Rene Van Weeren, Dietmar W Hutmacher, Travis J KleinAbstract:Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 °C, which is incompatible with most Biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in Biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.