The Experts below are selected from a list of 66615 Experts worldwide ranked by ideXlab platform
Gerald Thouand - One of the best experts on this subject based on the ideXlab platform.
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design of a toxicity biosensor based on aliivibrio fischeri entrapped in a disposable card
Environmental Science and Pollution Research, 2016Co-Authors: Sulivan Jouanneau, Mariejose Durandthouand, Gerald ThouandAbstract:The degradation of the marine environment is a subject of concern for the European authorities primarily because of its contamination by hydrocarbons. The traditional methods (ISO 11348 standard) of general toxicity assessment are unsuitable in a context of in situ monitoring, such as seaports or bathing zones. Consequently, to address this issue, bacterial Biosensors appear to be pertinent tools. This article presents the design of an innovative bioluminescent biosensor dedicated to in situ toxicity monitoring. This biosensor is based on the entrapment of the wild marine bioluminescent bacterial strain Aliivibrio fischeri ATCC® 49387™ in an agarose matrix within a disposable card. A pre-study was needed to select the most biological parameters. In particular, the regenerating medium’s composition and the hydrogel concentration needed for the bacterial entrapment (mechanical resistance) were optimized. Based on these data, the ability of the bacterial reporter to assess the sample toxicity was demonstrated using naphthalene as a chemical model. The biosensor’s results show a lower sensitivity to naphthalene (EC50 = 95 mg/L) compared with the results obtained using the reference method (EC50 = 43 mg/L). With this architecture, the biosensor is an interesting compromise among low maintenance, ease of use, appropriate sensitivity, relatively low cost and the ability to control online toxicity.
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design of a toxicity biosensor based on aliivibrio fischeri entrapped in a disposable card
Environmental Science and Pollution Research, 2016Co-Authors: Sulivan Jouanneau, Mariejose Durandthouand, Gerald ThouandAbstract:The degradation of the marine environment is a subject of concern for the European authorities primarily because of its contamination by hydrocarbons. The traditional methods (ISO 11348 standard) of general toxicity assessment are unsuitable in a context of in situ monitoring, such as seaports or bathing zones. Consequently, to address this issue, bacterial Biosensors appear to be pertinent tools. This article presents the design of an innovative bioluminescent biosensor dedicated to in situ toxicity monitoring. This biosensor is based on the entrapment of the wild marine bioluminescent bacterial strain Aliivibrio fischeri ATCC® 49387™ in an agarose matrix within a disposable card. A pre-study was needed to select the most biological parameters. In particular, the regenerating medium’s composition and the hydrogel concentration needed for the bacterial entrapment (mechanical resistance) were optimized. Based on these data, the ability of the bacterial reporter to assess the sample toxicity was demonstrated using naphthalene as a chemical model. The biosensor’s results show a lower sensitivity to naphthalene (EC50 = 95 mg/L) compared with the results obtained using the reference method (EC50 = 43 mg/L). With this architecture, the biosensor is an interesting compromise among low maintenance, ease of use, appropriate sensitivity, relatively low cost and the ability to control online toxicity.
Xiaowei Guo - One of the best experts on this subject based on the ideXlab platform.
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surface plasmon resonance based biosensor technique a review
Journal of Biophotonics, 2012Co-Authors: Xiaowei GuoAbstract:Optical Surface plasmon resonance (SPR) Biosensors represent the most advanced and developed optical label-free biosensor technology. Optical SPR Biosensors are a powerful detection and analysis tool that has vast applications in environmental protection, biotechnology, medical diagnostics, drug screening, food safety and security. This article reviews the recent development of SPR biosensor techniques, including bulk SPR and localized SPR (LSPR) Biosensors, for detecting interactions between an analyte of interest in solution and a biomolecular recognition. The concepts of bulk and localized SPs and the working principles of both sensing techniques are introduced. Major sensing advances on biorecognition elements, measurement formats, and sensing platforms are presented. Finally, the discussions on both biosensor techniques as well as comparison of both SPR sensing techniques are made. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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Surface plasmon resonance based biosensor technique: A review
Journal of Biophotonics, 2012Co-Authors: Xiaowei GuoAbstract:Optical Surface plasmon resonance (SPR) Biosensors represent the most advanced and developed optical label-free biosensor technology. Optical SPR Biosensors are a powerful detection and analysis tool that has vast applications in environmental protection, biotechnology, medical diagnostics, drug screening, food safety and security. This article reviews the recent development of SPR biosensor techniques, including bulk SPR and localized SPR (LSPR) Biosensors, for detecting interactions between an analyte of interest in solution and a biomolecular recognition. The concepts of bulk and localized SPs and the working principles of both sensing techniques are introduced. Major sensing advances on biorecognition elements, measurement formats, and sensing platforms are presented. Finally, the discussions on both biosensor techniques as well as comparison of both SPR sensing techniques are made.
Sulivan Jouanneau - One of the best experts on this subject based on the ideXlab platform.
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design of a toxicity biosensor based on aliivibrio fischeri entrapped in a disposable card
Environmental Science and Pollution Research, 2016Co-Authors: Sulivan Jouanneau, Mariejose Durandthouand, Gerald ThouandAbstract:The degradation of the marine environment is a subject of concern for the European authorities primarily because of its contamination by hydrocarbons. The traditional methods (ISO 11348 standard) of general toxicity assessment are unsuitable in a context of in situ monitoring, such as seaports or bathing zones. Consequently, to address this issue, bacterial Biosensors appear to be pertinent tools. This article presents the design of an innovative bioluminescent biosensor dedicated to in situ toxicity monitoring. This biosensor is based on the entrapment of the wild marine bioluminescent bacterial strain Aliivibrio fischeri ATCC® 49387™ in an agarose matrix within a disposable card. A pre-study was needed to select the most biological parameters. In particular, the regenerating medium’s composition and the hydrogel concentration needed for the bacterial entrapment (mechanical resistance) were optimized. Based on these data, the ability of the bacterial reporter to assess the sample toxicity was demonstrated using naphthalene as a chemical model. The biosensor’s results show a lower sensitivity to naphthalene (EC50 = 95 mg/L) compared with the results obtained using the reference method (EC50 = 43 mg/L). With this architecture, the biosensor is an interesting compromise among low maintenance, ease of use, appropriate sensitivity, relatively low cost and the ability to control online toxicity.
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design of a toxicity biosensor based on aliivibrio fischeri entrapped in a disposable card
Environmental Science and Pollution Research, 2016Co-Authors: Sulivan Jouanneau, Mariejose Durandthouand, Gerald ThouandAbstract:The degradation of the marine environment is a subject of concern for the European authorities primarily because of its contamination by hydrocarbons. The traditional methods (ISO 11348 standard) of general toxicity assessment are unsuitable in a context of in situ monitoring, such as seaports or bathing zones. Consequently, to address this issue, bacterial Biosensors appear to be pertinent tools. This article presents the design of an innovative bioluminescent biosensor dedicated to in situ toxicity monitoring. This biosensor is based on the entrapment of the wild marine bioluminescent bacterial strain Aliivibrio fischeri ATCC® 49387™ in an agarose matrix within a disposable card. A pre-study was needed to select the most biological parameters. In particular, the regenerating medium’s composition and the hydrogel concentration needed for the bacterial entrapment (mechanical resistance) were optimized. Based on these data, the ability of the bacterial reporter to assess the sample toxicity was demonstrated using naphthalene as a chemical model. The biosensor’s results show a lower sensitivity to naphthalene (EC50 = 95 mg/L) compared with the results obtained using the reference method (EC50 = 43 mg/L). With this architecture, the biosensor is an interesting compromise among low maintenance, ease of use, appropriate sensitivity, relatively low cost and the ability to control online toxicity.
Sergei V. Dzyadevych - One of the best experts on this subject based on the ideXlab platform.
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conductometric enzyme Biosensors based on natural zeolite clinoptilolite for urea determination
Materials Science and Engineering: C, 2011Co-Authors: O Y Saiapina, V M Pyeshkova, O O Soldatki, V G Melnik, Akata Kurc, Alai Walcarius, Sergei V. Dzyadevych, Nicole JaffrezicrenaulAbstract:Abstract Highly sensitive conductometric urea Biosensors were developed by exploiting the successful combination of ammonium-sieving and ion exchange properties of clinoptilolite, with a unique biorecognition capacity of urease. To optimize the performance of urea Biosensors based on clinoptilolite, the dependences of their analytical signals on pH, buffer capacity and ionic strength of phosphate buffer solution (PBS) were studied. Optimum pH for urea Biosensors was found within the range of pH 6.0–7.0. The dependences of Biosensors responses on buffer capacity and ionic strength of PBS were of the same profile as those obtained for the urea biosensor which was not modified with clinoptilolite. Analytical characteristics of urea Biosensors based on clinoptilolite were evaluated by determination of the sensitivity, linear and dynamic ranges, detection limit, the apparent Michaelis–Menten constant and the response time. The optimum features in terms of sensitivity, dynamic range, and detection limit (20.36 μS/mM, 0–64 mM, and 10 −6 M, respectively) were found for the urea biosensor, based on a primary layer of clinoptilolite followed by a secondary layer of urease and clinoptilolite in a single bioselective membrane. The apparent Michaelis–Menten constants K m for the developed urea Biosensors based on clinoptilolite varied from 2.73 to 5.67 mM. These values differed significantly from the value of K m for the urea biosensor which was not modified with zeolite (1.89 mM). All types of zeolite-modified Biosensors showed high operational and storage stability.
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application of amperometric Biosensors for analysis of ethanol glucose and lactate in wine
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Tatiana B Goriushkina, A P Soldatkin, Sergei V. DzyadevychAbstract:This article presents the application of amperometric Biosensors based on platinum printed electrodes SensLab and immobilized enzymes, alcohol oxidase, glucose oxidase, and lactate oxidase, for wine analysis. Created devices demonstrate linear response to ethanol, glucose, and lactate within the concentration range 0.3−20 mM, 0.04−2.5 mM, and 0.008−1 mM, respectively. No decrease in ethanol and glucose biosensor activity is revealed during 2 months after fabrication, and the operational stability of the lactate biosensor is sufficient only during 4 days. Developed Biosensors showed high selectivity to the substrate and are successfully applied to the analysis of such complex mixtures as wine and must. Good correlation of the results of analysis of different wines and must obtained by amperometric Biosensors with immobilized oxidases and traditional methods is shown. Created Biosensors can be used as a basis of a commercial device for express analysis of ethanol, glucose, and lactate in wine and must durin...
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development and optimisation of Biosensors based on ph sensitive field effect transistors and cholinesterases for sensitive detection of solanaceous glycoalkaloids
Biosensors and Bioelectronics, 2003Co-Authors: Valentyna N Arkhypova, Sergei V. Dzyadevych, A P Soldatkin, A V Elskaya, C Martelet, Nicole JaffrezicrenaultAbstract:Abstract Highly sensitive Biosensors based on pH-sensitive field effect transistors and cholinesterases for detection of solanaceous glycoalkaloids have been developed, characterised and optimised. The main analytical characteristics of the Biosensors developed have been studied under different conditions and an optimal experimental protocol for glycoalkaloids determination in model solution has been proposed. Using such a biosensor and an enzyme reversible inhibition effect, the total potato glycoalkaloids content can be determined within the range of 0.2–100 μM depending on the type of alkaloid, with lowest detection limits of 0.2 μM for α-chaconine, 0.5 μM for α-solanine and 1 μM for solanidine. The dynamic ranges for the compounds examined show that such Biosensors are suitable for a quantitative detection of glycoalkaloids in real potato samples. High reproducibility, operational and storage stability of the biosensor developed have been shown.
Michiyuki Matsuda - One of the best experts on this subject based on the ideXlab platform.
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booster a red shifted genetically encoded forster resonance energy transfer fret biosensor compatible with cyan fluorescent protein yellow fluorescent protein based fret Biosensors and blue light responsive optogenetic tools
ACS Sensors, 2020Co-Authors: Tetsuya Watabe, Kenta Terai, Kenta Sumiyama, Michiyuki MatsudaAbstract:Genetically encoded Forster resonance energy transfer (FRET)-based Biosensors have been developed for the visualization of signaling molecule activities. Currently, most of them are comprised of cyan and yellow fluorescent proteins (CFP and YFP), precluding the use of multiple FRET Biosensors within a single cell. Moreover, the FRET Biosensors based on CFP and YFP are incompatible with the optogenetic tools that operate at blue light. To overcome these problems, here, we have developed FRET Biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Forster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET Biosensors, we developed a FRET biosensor backbone named "Booster". The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV, a previously developed FRET biosensor comprising CFP and YFP. For the proof of concept, we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET Biosensors based on CFP and YFP. Second, we showed monitoring of PKA activation by Beggiatoa photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP. Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA. Collectively, the results demonstrate the effectiveness and versatility of Booster Biosensors as an imaging tool in vitro and in vivo.
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stable expression of fret Biosensors a new light in cancer research
Cancer Science, 2012Co-Authors: Kazuhiro Aoki, Naoki Komatsu, Eishu Hirata, Yuji Kamioka, Michiyuki MatsudaAbstract:The constituents of the oncogene signal transduction pathway are promising targets for anticancer drugs. Despite the wealth of available knowledge regarding their molecular properties, the spatiotemporal regulation of the signaling molecules remains elusive. Biosensors based on the principle of FRET have been developed to visualize the activities of the signaling molecules in living cells. However, difficulties in the development of sensitive FRET Biosensors have prevented their widespread use in cancer research. The lack of cell lines constitutively expressing a FRET biosensor has also limited their use. In this review, we will introduce the principle of FRET-based Biosensors, describe an optimized backbone of the FRET Biosensors, techniques to express FRET Biosensors stably in the cells, and discuss the future perspectives of FRET Biosensors in cancer research. (Cancer Sci 2012; 103: 614–619)