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Shen Hui-min - One of the best experts on this subject based on the ideXlab platform.

  • The study on the action mechanism of the extraction from Xanthium sibiricum to Botrytis Cinerea Pers
    Journal of Gansu Agricultural University, 2008
    Co-Authors: Shen Hui-min
    Abstract:

    Taking the strains of Botrytis Cinerea Pers as material,the action mechanism of the extraction from Xanthium sibiricum to Botrytis Cinerea Pers were studied by observation and physio-biochemical methods.The results showed that the extraction from Xanthium sibiricum had obvious inhibition on hypha growth,formation and germination of spores of Botrytis Cinerea Pers.When the concentration was 30 mg/mL,inhibiting rate to hypha growth of Botrytis Cinerea Pers reached 78.1 %,the inhibiting rates to formation and germinate of the spores reached 85.1 % and 77.4 %.The extracts of Xanthium sibiricum could result in leakage of inclusion of mycelium and change of penetrability of cell membrane so as to make conductivity increase.In addition,the content of protein from Botrytis Cinerea declined after a time under treatment.

  • Activity of the extract from Xanthium sibiricum callus on Botrytis Cinerea
    Journal of Gansu Agricultural University, 2008
    Co-Authors: Shen Hui-min
    Abstract:

    By using the methods of growth rate,spore germination and microscopical observation,taking nature and culture Xanthium sibiricum as CK,the activity of the extract from Xanthium sibiricum callus on Botrytis Cinerea was studied.The results showed that the extracts had high antifungal activity to Botrytis Cinerea.Its EC50 of growth inhibition of the hyphae was 20.27 mg/mL.When the concentration was 30 mg/mL,inhibiting rate of spore produce and germination of Botrytis Cinerea were over 70 %,EC50 of spore produce and germination were 9.06 mg/mL and 15.21 mg/mL respectively.The activity was comparable to the nature and culture Xanthium sibiricum extracts.

Michel Sancholle - One of the best experts on this subject based on the ideXlab platform.

Xing Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Propamidine decreas mitochondrial complex III activity of Botrytis Cinerea.
    BMB reports, 2010
    Co-Authors: Weibo Jin, Feng Juntao, Anliang Chen, Xing Zhang
    Abstract:

    Propamidine, an aromatic diamidine compound, is widely used as an antimicrobial agent. To uncover its mechanism on pathogenetic fungi, Botrytis Cinerea as an object was used to investigate effects of propamidine in this paper. The transmission electron microscope results showed that the mitochondrial membranes were collapsed after propamidine treatment, followed that mitochondria were disrupted. Inhibition of whole-cell and mitochondrial respiration by propamidine suggested that Propamidine is most likely an inhibitor of electron transport within Botrytis Cinerea mitochondria. Furthermore, the mitochondrial complex III activity were inhibited by propamidine.

Guoqing Li - One of the best experts on this subject based on the ideXlab platform.

  • Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis Cinerea
    Archives of Virology, 2017
    Co-Authors: Fangmin Hao, Mingde Wu, Ziliang Zhou, Guoqing Li
    Abstract:

    The complete sequence of a novel endornavirus (Botrytis Cinerea endornavirus 1, BcEV1) from the phytopathogenic fungus Botrytis Cinerea strain HBtom-372 was determined. The BcEV1 coding strand is 11,557 nucleotides long, possessing an open reading frame (ORF) that codes for a polyprotein of 3,787 amino acid residues and lacks a site-specific nick. The polyprotein contains viral methyltransferase (MTR) domain, a cysteine-rich region (CRR), two putative viral helicase (DEXDc-like and Hel-1) domains, and an RNA-dependent RNA polymerase_2 (RdRp_2) domain. In phylogenetic analysis, BcEV1 clustered with several fungal endornaviruses, forming an independent clade, and it was detected in 4.2 % of B. Cinerea strains collected from central China.

Seels B - One of the best experts on this subject based on the ideXlab platform.

  • Different products for biological control of Botrytis Cinerea examined on wounded stem tissue of tomato plants.
    Communications in agricultural and applied biological sciences, 2004
    Co-Authors: Gielen S, Aerts R, Seels B
    Abstract:

    For the moment the agents that are used against Botrytis Cinerea, in glasshouses were tomatoes are cultivated, are from chemical origin. For reducing the use of chemical agents in the future it is important to search for effective biological control agents against the fight of Botrytis cinerae. The following biological products Vital pasta, Vital gel and Elot-Vis were examined in there possibility to control Botrytis Cinerea. Elot Vis was tested out in experiments that were carried out in climate chambers were leafs of 3 week old tomato plants were artificially infected with Botrytis Cinerea spores. Also the biological products of Vital were first investigated in experiments that were carried out in climate chambers. In stead off leafs of tomato plants it were stem wounds of tomato plants who were treated with the pasta or the gel that was spread over the wounded surface after this has been inoculated with a suspension of conidia of Botrytis cinerae. The results of these first tests that were executed in the climate chambers were the circumstances for Botrytis Cinerea were ideal seemed promising. In the next step these products were tested out on large scale in glasshouses. For each plant 5 wounds were created by removing the leafs, these wounds were or first treated with the Biological product and thereafter artificially infected with Botrytis Cinerea spores to check out if these products can be used as a preventive agent or the wounds were first inoculated with a suspension of Botrytis Cinerea spores and thereafter treated with the product. For the product Elot-Vis a few plants were totally sprayed with an Elot-Vis suspension before leafs were removed and the wounds were inoculated with conidia of Botrytis Cinerea to check out if this product was able to activated the induced systemic resistance pathway. The experiments that were executed in glasshouses showed different percentages of succeeded Botrytis Cinerea infections. This is probable due to the different weather conditions during both days that the experiments were executed. For the wounds that were treated with pasta it was difficult to distinguish wounds were Botrytis Cinerea succeeded to infect the plant, because these wounds frequently didn't show any sign of infection on the surface but when the wounds with the pasta were cut open it was possible to see Botrytis Cinerea infections inside the stem.

  • Biocontrol agents of Botrytis Cinerea tested in climate chambers by making artificial infection on tomato leafs.
    Communications in agricultural and applied biological sciences, 2004
    Co-Authors: Gielen S, Aerts R, Seels B
    Abstract:

    To reduce the use of chemical agents, that are causing damage to the environment, in the fight against Botrytis Cinerea, different BCA's were tested for their possibility to control Botrytis Cinerea in a biological way. In order to investigate the effectiveness of the different micro organisms and Elot-Vis, experiments were carried out in climate chambers with 5 weeks old tomato plants. Leafs on the plant were inoculated with drops of a suspension that contained spores of Botrytis Cinerea. The possible antagonists that were tested in these experiments were Trichoderma harzianum (Trichodex), T. asperellum (Biofungus), T. hamatum (T382), Bacillus subtilis (Serenade and Phytovit) and Pseudomonas aeruginosa (7NSK2 and KMPCH). For all these different micro organisms the direct and the indirect influence on Botrytis Cinerea was investigated. In tests where the direct influence of the antagonists was examined, the spores of the moulds or the bacteria were suspended together with spores of Botrytis Cinerea and subsequently drops of this suspension were pippeted on the leafs. After a while by ideal circumstances for Botrytis Cinerea the infections on the inoculated leafs were counted. For the indirect influence of the antagonists, also leafs of 5 weeks old tomato plants were inoculated with a suspension of Botrytis Cinerea spores. The roots of the tomato plants that were used for testing the indirect influence were treated during there growth with a suspension of the antagonist to see if induced systemic resistance pathway (ISR) was activated. For testing the effectiveness of Elot-Vis, tomato plants were sprayed a few times with a solution of this product during their growth. Results of the climate chamber test of the plants that were treated with Elot-Vis, showed a reduction of Botrytis Cinerea infections on the inoculated leafs. Biological control agents seem to be not always very effective against Botrytis Cinerea. The biological control agents that are containing micro organisms are also depending on the circumstances of the environment for an optimal development. These conditions are not always that optimal to compete with Botrytis Cinerea or other micro organisms that are present on the plant.

  • Development of a selective medium for the determination of the spore concentrations of Botrytis Cinerea in the air.
    Communications in agricultural and applied biological sciences, 2003
    Co-Authors: Gielen S, Aerts R, Seels B
    Abstract:

    Gray mold, caused by Botrytis Cinerea, is an important disease that causes world-wide extensive damage to a wide range of economically important crops. When it is necessary to determine the spore concentration of Botrytis Cinerea in a certain area, it is important to develop a method that can capture the spores of Botrytis Cinerea and that can identify them. For the identification and enumeration of the plant pathogen Botrytis Cinerea in the environment the easiest method available for the moment is the use of a selective medium. Several selective media for the isolation of Botrytis spp. have been developed by other research groups. All these media contain fungicides that are usually non-toxic towards Botrytis species and tannic acid, which is oxidized to produce a brown pigment that visualises the growth of Botrytis Cinerea on the selective media. It seemed that different isolates of Botrytis Cinerea that are found in nature have different sensitivities towards the different fungicide concentrations that are used in the selective media. Making the "optimal" selective media for Botrytis Cinerea, we have to take in consideration that so many as possible Botrytis Cinerea isolates must be able to germinate and grow on this selective medium and that the contamination of other micro-organisms on the selective medium must be minimized. Before the final composition of our selective medium for Botrytis Cinerea, different combinations of fungicide concentrations were tried out of the following three fungicides: Rubigan, maneb and PCNB (pentachloronitrobenzene). All these selective media with different fungicides concentrations were tested out for spore germination and mycelium growth of Botrytis Cinerea. Because it was obvious that the percentage Botrytis Cinerea that germinated on PDA (Potato Dextrose Agar) was higher than on the selective medium a few experiments were executed in which the percentage of spore germination on PDA was compared with the percentage of spore germination on selective media. From the results of these experiments a correction factor was calculated that will be used when spore concentrations have to be determined for air detections that are carried out in glasshouses.