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Leyu Wang - One of the best experts on this subject based on the ideXlab platform.

  • a biosynthesized gold nanoparticle from staphylococcus aureus as a functional factor in muscle tissue engineering
    Applied Materials Today, 2021
    Co-Authors: Renjie Qiu, Weirong Xiong, Wenxi Hua, Xiaomin Sun, Malcolm Xing, Leyu Wang
    Abstract:

    Abstract Nano-biosynthesis for gold nanoparticle (AuNP) using bacteria can produce the tailored functional AuNP because of the different bioactive molecules coating on the AuNP derived from different bacteria. However, the biosynthesis for the biocompatible AuNP from the harmful bacteria is still challenging, and the extensive application of the bacteria-derived AuNP in tissue repair is lacking. In this study, without other auxiliary chemical molecules, the gradient centrifugation was used to successfully remove the toxic part of the pristine AuNPs biosynthesized from Staphylococcus aureus (S. aureus). The purified S. aureus-derived AuNPs were proved to be beneficial for the muscle cells’ viability and could even protect the cells against the cardiotoxin damages. Furthermore, the S. aureus-derived AuNPs were assembled into an elastic scaffold to form the AuNPs-incorporated Cardiac Patch. The in vivo study in rat myocardial infarction (MI) models demonstrated that these S. aureus-derived AuNPs could be taken as a functional factor in the Cardiac Patch to promote MI repair, through decreasing the infarct area and improving the Cardiac function of the infarct heart. This study provides a functional S. aureus-derived AuNP with tissue repair potential, which can be extensively applied in muscle tissue engineering.

  • chitosan calcium silicate Cardiac Patch stimulates cardiomyocyte activity and myocardial performance after infarction by synergistic effect of bioactive ions and aligned nanostructure
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Xiaotong Wang, Leyu Wang, Huang-tian Yang, Xiaozhong Qiu, Feng Bao, Jiang Chang
    Abstract:

    Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on Cardiac cell behaviors and Cardiac tissue repair. In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of Cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm. Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite Cardiac Patch scaffolds. These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of Cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures. The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan Cardiac Patch evidently improved Cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats. Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite Cardiac Patch may be a promising scaffold for repair of infarcted myocardium.

  • In situ pPy-modification of chitosan porous membrane from mussel shell as a Cardiac Patch to repair myocardial infarction
    Applied Materials Today, 2019
    Co-Authors: Xiaoping Song, Leyu Wang, Jie Mei, Annada Ananth, Xiaozhong Qiu
    Abstract:

    Abstract Polypyrrole (pPy), a widely-used conductive material, can create an electrophysiological condition for the exogenous cardiomyocytes (CMs) via its conductivity, biocompatibility and available processing method. Our previously developed flexible scaffold from the mussel-derived chitosan shell possessed multiscale, interconnected-porous structure and proper stiffness, which was a promising tissue substitute for wound healing. Here, a 3D pristine hybrid scaffold was fabricated by incorporating polypyrrole into the mussel shell-derived membrane (shell-pPy). The pyrrole monomers were grafted onto to the shell membrane and then in situ polymerized to form conjugated pPy-chitosan shell using FeCl3. The developed pPy-chitosan shell was used to produce an engineered Cardiac Patch (ECP). The shell-pPy ECP maintained subtle spatial structure and was granted well cellular viability, aligned morphology, abundant striation and organized CX43, robust contraction and rapid calcium transients in vitro. When transplanted into the infarcted hearts, the developed shell-pPy ECP could also mimic the mechano-electronic function for cardiomyocytes maturation and integrity. Notably, obvious angiogenesis was triggered, and Cardiac pumping performance was promoted in the infarcted area by the shell-pPy ECP. The study highlighted a functional pPy-modified Cardiac Patch with suitable conductivity, subtle 3D structure, and mechanical property can serve as an ideal ECP to mimic Cardiac niche for myocardial repair.

  • Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure
    ACS applied materials & interfaces, 2018
    Co-Authors: Wang Xiaotong, Leyu Wang, Bao Feng, Huang-tian Yang, Xiaozhong Qiu, Jiang Chang
    Abstract:

    Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on Cardiac cell behaviors and Cardiac tissue repair. In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of Cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm. Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite Cardiac Patch scaffolds. These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of Cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures. The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan Cardiac Patch evidently improved Cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats. Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite Cardiac Patch may be a promising scaffold for repair of infarcted myocardium.

  • mussel inspired conductive cryogel as Cardiac tissue Patch to repair myocardial infarction by migration of conductive nanoparticles
    Advanced Functional Materials, 2016
    Co-Authors: Wenxi Hua, Leyu Wang, Xiaoping Song, Junzi Jiang, Ali Darabi, Chen Song, Wen Zhong
    Abstract:

    The engineered Cardiac Patch (ECP) is a promising strategy to repair infarct myocardium and restore the Cardiac function. An ideal ECP should be able to mimic the primary attributes of native myocardium, which includes a high resilience, good cardiomyocyte adhesion, and synchronous contraction. Here, a mussel-inspired dopamine crosslinker is used to integrate polypyrrole (Ppy) nanoparticles, gelatin-methyacrylate, and poly(ethylene glycol) diacrylate into a cryogel form. The dopamine crosslinker and Ppy nanoparticles are coordinated to obtain optimal mechanical and superelastic properties for the ECP. The dopamine facilitates the uniform distribution of the Ppy nanoparticles, which migrate and fuse from the scaffold to the surface of the cardiomyocytes, revealing a potential mechanism for restoring infarct myocardium. The incorporated Ppy nanoparticles thus significantly enhance the functionalization of the cardiomyocytes, resulting in excellent synchronous contraction by increasing the expression of α-actinin and CX-43. Cardiomyocytes-loaded ECP can improve the Cardiac function in myocardial-infarction (MI) affected rat models. The results show that the fractional shortening and ejection fraction are elevated by about 50% and that the infarct size is reduced by 42.6%. Collectively, this study highlights an effective Cardiac Patch based on mussel-inspired conductive particle adhesion and a superelastic cryogel promising for the restoration of infarcted myocardium.

Tal Dvir - One of the best experts on this subject based on the ideXlab platform.

  • Gold Nanorod-Based Engineered Cardiac Patch for Suture-Free Engraftment by Near IR
    Nano letters, 2018
    Co-Authors: Maayan Malki, Sharon Fleischer, Assaf Shapira, Tal Dvir
    Abstract:

    Although Cardiac Patches hold a promise for repairing the infarcted heart, their integration with the myocardium by sutures may cause further damage to the diseased organ. To address this issue, we developed facile and safe, suture-free technology for the attachment of engineered tissues to organs. Here, nanocomposite scaffolds comprised of albumin electrospun fibers and gold nanorods (AuNRs) were developed. Cardiac cells were seeded within the scaffolds and assembled into a functioning Patch. The engineered tissue was then positioned on the myocardium and irradiated with a near IR laser (808 nm). The AuNRs were able to absorb the light and convert it to thermal energy, which locally changed the molecular structure of the fibrous scaffold, and strongly, but safely, attached it to the wall of the heart. Such hybrid biomaterials can be used in the future to integrate any engineered tissue with any defected organs, while minimizing the risk of additional injury for the patient, caused by the conventional stitching methods.

  • gold nanorod based engineered Cardiac Patch for suture free engraftment by near ir
    Nano Letters, 2018
    Co-Authors: Maayan Malki, Sharon Fleischer, Assaf Shapira, Tal Dvir
    Abstract:

    Although Cardiac Patches hold a promise for repairing the infarcted heart, their integration with the myocardium by sutures may cause further damage to the diseased organ. To address this issue, we developed facile and safe, suture-free technology for the attachment of engineered tissues to organs. Here, nanocomposite scaffolds comprised of albumin electrospun fibers and gold nanorods (AuNRs) were developed. Cardiac cells were seeded within the scaffolds and assembled into a functioning Patch. The engineered tissue was then positioned on the myocardium and irradiated with a near IR laser (808 nm). The AuNRs were able to absorb the light and convert it to thermal energy, which locally changed the molecular structure of the fibrous scaffold, and strongly, but safely, attached it to the wall of the heart. Such hybrid biomaterials can be used in the future to integrate any engineered tissue with any defected organs, while minimizing the risk of additional injury for the patient, caused by the conventional sti...

  • Engineered hybrid Cardiac Patches with multifunctional electronics for online monitoring and regulation of tissue function
    Nature Materials, 2016
    Co-Authors: Ron Feiner, Sharon Fleischer, Idan Gal, Mohammed Malki, Assaf Shapira, Leeya Engel, Yosi Shacham-diamand, Tal Dvir
    Abstract:

    In Cardiac tissue engineering approaches to treat myocardial infarction, Cardiac cells are seeded within three-dimensional porous scaffolds to create functional Cardiac Patches. However, current Cardiac Patches do not allow for online monitoring and reporting of engineered-tissue performance, and do not interfere to deliver signals for Patch activation or to enable its integration with the host. Here, we report an engineered Cardiac Patch that integrates Cardiac cells with flexible, freestanding electronics and a 3D nanocomposite scaffold. The Patch exhibited robust electronic properties, enabling the recording of cellular electrical activities and the on-demand provision of electrical stimulation for synchronizing cell contraction. We also show that electroactive polymers containing biological factors can be deposited on designated electrodes to release drugs in the Patch microenvironment on demand. We expect that the integration of complex electronics within Cardiac Patches will eventually provide therapeutic control and regulation of Cardiac function.

  • Creating Unique Cell Microenvironments for the Engineering of a Functional Cardiac Patch
    Myocardial Tissue Engineering, 2010
    Co-Authors: Tal Dvir, Jonathan Leor, Smadar Cohen
    Abstract:

    Tissue engineering is an approach used to create a functional Cardiac Patch for the purpose of scar support after a myocardial infarct (MI). Cardiac cells, or cells of other sources, are seeded into scaffolds, which provide an artificial biomechanical support until the cells secrete extracellular matrix and regenerate into a functional tissue. In this chapter we describe the creative design of various cell microenvironments, which promote the development of a thick vascularized Cardiac Patch, ready to face the harsh conditions of the infarcted heart. Among these microenvironments are unique bioreactor systems that increase mass transfer through the developing Cardiac tissue at the in vitro engineering stage and the use of various vascularization techniques, including the use of the body as a bioreactor to induce rapid vascularization prior to transplantation on the infarcted heart.

  • prevascularization of Cardiac Patch on the omentum improves its therapeutic outcome
    Proceedings of the National Academy of Sciences of the United States of America, 2009
    Co-Authors: Tal Dvir, Alon Kedem, Emil Ruvinov, Oren Levy, Inbar Freeman, Natalie Landa, Radka Holbova, Micha S Feinberg, Shani Dror, Yoram Etzion
    Abstract:

    The recent progress made in the bioengineering of Cardiac Patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a Cardiac Patch with mature vasculature by heterotopic transplantation onto the omentum. The Patch was constructed by seeding neonatal Cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the Patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized Cardiac Patch showed structural and electrical integration into host myocardium. Moreover, the vascularized Patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized Cardiac Patch into infarct can improve Cardiac function after MI.

Gianluigi Condorelli - One of the best experts on this subject based on the ideXlab platform.

  • electroactive polyurethane siloxane derived from castor oil as a versatile Cardiac Patch part ii hl 1 cytocompatibility and electrical characterizations
    Journal of Biomedical Materials Research Part A, 2016
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    In first part of this experiment, biocompatibility of the newly developed electroactive polyurethane/siloxane films containing aniline tetramer moieties was demonstrated with proliferation and differentiation of C2C12 myoblasts. Here we further assessed the cytocompatibility of the prepared samples with HL1-cell line, the electrophysiological properties and the Patch clamp recording of the seeded cells over the selected electroactive sample. Presence of electroactive aniline tetramer in the structure of polyurethane/siloxane led to the increased expression of Cardiac-specific genes of HL-1 cells involved in muscle contraction and electrical coupling. Our results showed that expression of Cx43, TrpT-2, and SERCA genes was significantly increased in conductive sample compared to tissue culture plate and the corresponding non-conductive analogous. The prepared materials were not only biocompatible in terms of cellular toxicity, but did not alter the intrinsic electrical characteristics of HL-1 cells. Embedding the electroactive moiety into the prepared films improved the properties of these polymeric Cardiac construct through the enhanced transmission of electrical signals between the cells. Based on morphological observation, calcium imaging and electrophysiological recordings, we demonstrated the potential applicability of these materials for Cardiac tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1398-1407, 2016.

  • electroactive polyurethane siloxane derived from castor oil as a versatile Cardiac Patch part i synthesis characterization and myoblast proliferation and differentiation
    Journal of Biomedical Materials Research Part A, 2016
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    Tissue-engineered Cardiac Patch aims at regenerating an infarcted heart by improving Cardiac function and providing mechanical support to the diseased myocardium. In order to take advantages of electroactivity, a new synthetic method was developed for the introduction of an electroactive oligoaniline into the backbone of prepared Patches. For this purpose, a series of electroactive polyurethane/siloxane films containing aniline tetramer (AT) was prepared through sol-gel reaction of trimethoxysilane functional intermediate polyurethane prepolymers made from castor oil and poly(ethylene glycol). Physicochemical, mechanical, and electrical conductivity of samples were evaluated and the recorded results were correlated to their structural characteristics. The optimized films were proved to be biodegradable and have tensile properties suitable for Cardiac Patch application. The embedded AT moieties in the backbone of the prepared samples preserved their electroactivity with the electrical conductivity in the range of 10-4 S/cm. The prepared films were compatible with proliferation of C2C12 and had potential for enhancing myotube formation even without external electrical stimulation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 775-787, 2016.

  • Electroactive polyurethane/siloxane derived from castor oil as a versatile Cardiac Patch, part I: Synthesis, characterization, and myoblast proliferation and differentiation.
    Journal of biomedical materials research. Part A, 2015
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    Tissue-engineered Cardiac Patch aims at regenerating an infarcted heart by improving Cardiac function and providing mechanical support to the diseased myocardium. In order to take advantages of electroactivity, a new synthetic method was developed for the introduction of an electroactive oligoaniline into the backbone of prepared Patches. For this purpose, a series of electroactive polyurethane/siloxane films containing aniline tetramer (AT) was prepared through sol-gel reaction of trimethoxysilane functional intermediate polyurethane prepolymers made from castor oil and poly(ethylene glycol). Physicochemical, mechanical, and electrical conductivity of samples were evaluated and the recorded results were correlated to their structural characteristics. The optimized films were proved to be biodegradable and have tensile properties suitable for Cardiac Patch application. The embedded AT moieties in the backbone of the prepared samples preserved their electroactivity with the electrical conductivity in the range of 10-4 S/cm. The prepared films were compatible with proliferation of C2C12 and had potential for enhancing myotube formation even without external electrical stimulation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 775-787, 2016.

Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.

  • Elastomeric Core/Shell Nanofibrous Cardiac Patch as a Biomimetic Support for Infarcted Porcine Myocardium
    Tissue engineering. Part A, 2015
    Co-Authors: Rajeswari Ravichandran, Jayarama Reddy Venugopal, Shayanti Mukherjee, Subramanian Sundarrajan, Seeram Ramakrishna
    Abstract:

    Heart failure due to Myocardial Infarction (MI) remains the leading cause of death worldwide due to the inability of myocardial tissue to regenerate following infarction. Current therapies could only retard the progression of disease, but fails to bring functional improvement and Cardiac regeneration. The present study analyzes the potentials of Poly(glycerol sebacate)/Fibrinogen (PGS/Fib) core/shell fibers as a structural support and initial entrapment of cells in an in vivo porcine model using echocardiography, histology, and immunohistochemistry. The echocardiography results showed the increased ejection fraction (EF) in PGS/Fib/VEGF/Cells compared with MI controls. The percentage increase in the End Diastolic Volume (EDV) dimension from post MI period to 4 weeks follow-up was the least in PGS/Fib/VEGF/Cells groups compared with MI and cell control group proving that the PGS/Fib/VEGF/Cells group restored the left ventricle (LV) function after MI, evident from the improvement in EF and prevention of LV enlargement. Further, immunohistochemistry results demonstrated that most of the transplanted mesenchymal stem cells (MSCs) within the PGS/Fib/VEGF scaffolds expressed Cardiac marker proteins troponin and actinin and endothelial cell marker protein CD31 indicating differentiation of human bone marrow MSCs into Cardiac cells and endothelial cells. The developed nanofibrous Cardiac Patch PGS/Fib/VEGF/Cells provides both functional and structural integrity to the infarcted myocardium and also serves as a suitable matrix for the entrapment of MSCs in clinical applications for Cardiac tissue engineering.

  • elastomeric core shell nanofibrous Cardiac Patch as a biomimetic support for infarcted porcine myocardium
    Tissue Engineering Part A, 2015
    Co-Authors: Rajeswari Ravichandran, Jayarama Reddy Venugopal, Shayanti Mukherjee, Subramanian Sundarrajan, Seeram Ramakrishna
    Abstract:

    Heart failure due to Myocardial Infarction (MI) remains the leading cause of death worldwide due to the inability of myocardial tissue to regenerate following infarction. Current therapies could only retard the progression of disease, but fails to bring functional improvement and Cardiac regeneration. The present study analyzes the potentials of Poly(glycerol sebacate)/Fibrinogen (PGS/Fib) core/shell fibers as a structural support and initial entrapment of cells in an in vivo porcine model using echocardiography, histology, and immunohistochemistry. The echocardiography results showed the increased ejection fraction (EF) in PGS/Fib/VEGF/Cells compared with MI controls. The percentage increase in the End Diastolic Volume (EDV) dimension from post MI period to 4 weeks follow-up was the least in PGS/Fib/VEGF/Cells groups compared with MI and cell control group proving that the PGS/Fib/VEGF/Cells group restored the left ventricle (LV) function after MI, evident from the improvement in EF and prevention of LV enlargement. Further, immunohistochemistry results demonstrated that most of the transplanted mesenchymal stem cells (MSCs) within the PGS/Fib/VEGF scaffolds expressed Cardiac marker proteins troponin and actinin and endothelial cell marker protein CD31 indicating differentiation of human bone marrow MSCs into Cardiac cells and endothelial cells. The developed nanofibrous Cardiac Patch PGS/Fib/VEGF/Cells provides both functional and structural integrity to the infarcted myocardium and also serves as a suitable matrix for the entrapment of MSCs in clinical applications for Cardiac tissue engineering.

  • biocompatibility evaluation of electrically conductive nanofibrous scaffolds for Cardiac tissue engineering
    Journal of Materials Chemistry B, 2013
    Co-Authors: Molamma P Prabhakaran, Seeram Ramakrishna
    Abstract:

    Myocardial tissue engineering offers a novel technology to improve or regenerate Cardiac functions using a combination of cells, biomaterials and engineering strategies. Inspired by low-resistance pathways for electrical signal propagation in the native heart tissue, electrically conductive nanofibrous scaffolds composed of melanin, poly(L-lactide-co-e-caprolactone) and gelatin were fabricated to provide electrophysiological cues to Cardiac myocytes and mimic the native myocardial environment. Our results show that by increasing the concentration of melanin to 40% within the composite, the fiber diameters reduced to 153 ± 30 nm, modulus decreased to 7.1 ± 0.6 MPa, and conductance increased to 259.51 ± 187.60 μS cm−1. Results of cell proliferation and immunostaining analysis of human Cardiac myocytes demonstrated that the conductive nanofibers containing 10% melanin promote cell interaction with expression of Cardiac-specific proteins compared to other scaffolds. Electrical stimulation through the scaffolds showed enhanced cell proliferation and the expression of connexin-43, signifying the potential of using melanin containing nanofibers as a suitable Cardiac Patch for the regeneration of infarct myocardium.

  • electrospun biocomposite nanofibrous Patch for Cardiac tissue engineering
    Biomedical Materials, 2011
    Co-Authors: Molamma P Prabhakaran, Dan Kai, Laleh Ghasemimobarakeh, Seeram Ramakrishna
    Abstract:

    A bioengineered construct that matches the chemical, mechanical, biological properties and extracellular matrix morphology of native tissue could be suitable as a Cardiac Patch for supporting the heart after myocardial infarction. The potential of utilizing a composite nanofibrous scaffold of poly(dl-lactide-co-glycolide)/gelatin (PLGA/Gel) as a biomimetic Cardiac Patch is studied by culturing a population of cardiomyocyte containing cells on the electrospun scaffolds. The chemical characterization and mechanical properties of the electrospun PLGA and PLGA/Gel nanofibers were studied by Fourier transform infrared spectroscopy, scanning electron microscopy and tensile measurements. The biocompatibility of the scaffolds was also studied and the cardiomyocytes seeded on PLGA/Gel nanofibers were found to express the typical functional Cardiac proteins such as alpha-actinin and troponin I, showing the easy integration of cardiomyocytes on PLGA/Gel scaffolds. Our studies strengthen the application of electrospun PLGA/Gel nanofibers as a bio-mechanical support for injured myocardium and as a potential substrate for induction of endogenous cardiomyocyte proliferation, ultimately reducing the Cardiac dysfunction and improving Cardiac remodeling.

Nafiseh Baheiraei - One of the best experts on this subject based on the ideXlab platform.

  • Development of a Novel Electroactive Cardiac Patch Based on Carbon Nanofibers and Gelatin Encouraging Vascularization.
    Applied biochemistry and biotechnology, 2019
    Co-Authors: Arezou Mehrabi, Nafiseh Baheiraei, Adabi, Zahra Amirkhani
    Abstract:

    Tissue engineering makes it possible to fabricate scaffolds that can help the function of defective tissues or even the most complex organs such as the heart. Carbon nanofibers (CNFs), because of their high mechanical strength and electrical properties, can improve the functional coupling of cardiomyocytes and their electrophysiological properties. In this study, electroactive CNF/gelatin (Gel) nanofibrous Cardiac Patches were prepared by an electrospinning method. Scanning electron microscope (SEM) evaluation of prepared scaffolds showed randomly oriented nanofibers. The electrical conductivity of the CNF/Gel scaffolds was assessed by a four-probe device and was in the semiconducting range (~ 10-5 S/m). The result of an MTT assay confirmed the excellent biocompatibility of electroactive CNF/Gel scaffolds. Also, CNF-containing scaffolds supported cardiomyocyte adhesion and increased expression of the Cardiac genes including TrpT-2, Actn4, and Conx43 compared with the non-conductive counterpart. Our findings also confirmed the angiogenic potential of CNF/Gel scaffolds as compatible and electroactive platforms for Cardiac tissue engineering.

  • Electroactive Cardiac Patch containing reduced graphene oxide with potential antibacterial properties
    Materials science & engineering. C Materials for biological applications, 2019
    Co-Authors: Mohammad Hadi Norahan, Mohadeseh Pourmokhtari, Mohammad Reza Saeb, Bita Bakhshi, Mina Soufi Zomorrod, Nafiseh Baheiraei
    Abstract:

    Graphene based nanomaterials are promising candidates for Cardiac tissue engineering due to the excellent electrical and mechanical properties and the robust surface chemistry. This research was designed to investigate the physicochemical and biological effects of increasing concentration of reduced graphene oxide (rGO) coating on collagen (Col) scaffolds as well as their antibacterial properties. Enhanced GO coating content to 400 μg/ml and its reduction showed improvement of HUVECs viability, however, following reduction of more GO concentration, decreased cell viability was observed. Compared with the Col counterpart, electroactive containing rGO scaffolds upregulated Cardiac gene expression involve in electrical coupling (Cx43), muscle contraction and relaxation (troponin-T) and cytoskeleton alignment (actinin-4) after 7 days even without external electrical stimulation. rGO coating significantly improved mechanical properties and the electroactivity of the Col scaffolds reaching to 1100 ± 31 kPa and 4 × 10-4 ± 1.20 S/m for GO concentration of 800 μg/ml, respectively. Also, the antibacterial properties of Col-rGO-400 scaffolds against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes were confirmed by culture and FESEM observation. Taken together, the results indicated that rGO coating presents promising properties to Col scaffolds providing a desirable micro environment for cardiomyocytes coupling and gene upregulation as well as antibacterial activities for Cardiac Patch application.

  • electroactive polyurethane siloxane derived from castor oil as a versatile Cardiac Patch part ii hl 1 cytocompatibility and electrical characterizations
    Journal of Biomedical Materials Research Part A, 2016
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    In first part of this experiment, biocompatibility of the newly developed electroactive polyurethane/siloxane films containing aniline tetramer moieties was demonstrated with proliferation and differentiation of C2C12 myoblasts. Here we further assessed the cytocompatibility of the prepared samples with HL1-cell line, the electrophysiological properties and the Patch clamp recording of the seeded cells over the selected electroactive sample. Presence of electroactive aniline tetramer in the structure of polyurethane/siloxane led to the increased expression of Cardiac-specific genes of HL-1 cells involved in muscle contraction and electrical coupling. Our results showed that expression of Cx43, TrpT-2, and SERCA genes was significantly increased in conductive sample compared to tissue culture plate and the corresponding non-conductive analogous. The prepared materials were not only biocompatible in terms of cellular toxicity, but did not alter the intrinsic electrical characteristics of HL-1 cells. Embedding the electroactive moiety into the prepared films improved the properties of these polymeric Cardiac construct through the enhanced transmission of electrical signals between the cells. Based on morphological observation, calcium imaging and electrophysiological recordings, we demonstrated the potential applicability of these materials for Cardiac tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1398-1407, 2016.

  • electroactive polyurethane siloxane derived from castor oil as a versatile Cardiac Patch part i synthesis characterization and myoblast proliferation and differentiation
    Journal of Biomedical Materials Research Part A, 2016
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    Tissue-engineered Cardiac Patch aims at regenerating an infarcted heart by improving Cardiac function and providing mechanical support to the diseased myocardium. In order to take advantages of electroactivity, a new synthetic method was developed for the introduction of an electroactive oligoaniline into the backbone of prepared Patches. For this purpose, a series of electroactive polyurethane/siloxane films containing aniline tetramer (AT) was prepared through sol-gel reaction of trimethoxysilane functional intermediate polyurethane prepolymers made from castor oil and poly(ethylene glycol). Physicochemical, mechanical, and electrical conductivity of samples were evaluated and the recorded results were correlated to their structural characteristics. The optimized films were proved to be biodegradable and have tensile properties suitable for Cardiac Patch application. The embedded AT moieties in the backbone of the prepared samples preserved their electroactivity with the electrical conductivity in the range of 10-4 S/cm. The prepared films were compatible with proliferation of C2C12 and had potential for enhancing myotube formation even without external electrical stimulation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 775-787, 2016.

  • Electroactive polyurethane/siloxane derived from castor oil as a versatile Cardiac Patch, part I: Synthesis, characterization, and myoblast proliferation and differentiation.
    Journal of biomedical materials research. Part A, 2015
    Co-Authors: Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
    Abstract:

    Tissue-engineered Cardiac Patch aims at regenerating an infarcted heart by improving Cardiac function and providing mechanical support to the diseased myocardium. In order to take advantages of electroactivity, a new synthetic method was developed for the introduction of an electroactive oligoaniline into the backbone of prepared Patches. For this purpose, a series of electroactive polyurethane/siloxane films containing aniline tetramer (AT) was prepared through sol-gel reaction of trimethoxysilane functional intermediate polyurethane prepolymers made from castor oil and poly(ethylene glycol). Physicochemical, mechanical, and electrical conductivity of samples were evaluated and the recorded results were correlated to their structural characteristics. The optimized films were proved to be biodegradable and have tensile properties suitable for Cardiac Patch application. The embedded AT moieties in the backbone of the prepared samples preserved their electroactivity with the electrical conductivity in the range of 10-4 S/cm. The prepared films were compatible with proliferation of C2C12 and had potential for enhancing myotube formation even without external electrical stimulation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 775-787, 2016.