The Experts below are selected from a list of 85962 Experts worldwide ranked by ideXlab platform

Stephen A. Boppart - One of the best experts on this subject based on the ideXlab platform.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging.
    Biomedical optics express, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Andrew J. Bower, P. Scott Carney, Stephen A. Boppart
    Abstract:

    Three-dimensional high-resolution Imaging methods are important for Cellular-level research. Optical coherence microscopy (OCM) is a low-coherence-based interferometry technology for Cellular Imaging with both high axial and lateral resolution. Using a high-numerical-aperture objective, OCM normally has a shallow depth of field and requires scanning the focus through the entire region of interest to perform volumetric Imaging. With a higher-numerical-aperture objective, the image quality of OCM is affected by and more sensitive to aberrations. Interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) are computed Imaging techniques that overcome the depth-of-field limitation and the effect of optical aberrations in optical coherence tomography (OCT), respectively. In this work we combine OCM with ISAM and CAO to achieve high-speed volumetric Cellular Imaging. Experimental Imaging results of ex vivo human breast tissue, ex vivo mouse brain tissue, in vitro fibroblast cells in 3D scaffolds, and in vivo human skin demonstrate the significant potential of this technique for high-speed volumetric Cellular Imaging.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging
    Biomedical Optics 2014, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Paul Scott Carney, Stephen A. Boppart
    Abstract:

    We demonstrate the combination of interferometric synthetic aperture microscopy (ISAM), computational adaptive optics (CAO) and coherence tomography microscopy (OCM) provides a high-speed volumetric Cellular Imaging.

Shuming Nie - One of the best experts on this subject based on the ideXlab platform.

  • Engineering Luminescent Quantum Dots for In Vivo Molecular and Cellular Imaging
    Annals of Biomedical Engineering, 2006
    Co-Authors: Andrew M. Smith, Gang Ruan, Matthew N. Rhyner, Shuming Nie
    Abstract:

    Semiconductor quantum dots are luminescent nanoparticles that are under intensive development for use as a new class of optical Imaging contrast agents. Their novel properties such as optical tunability, improved photostability, and multicolor light emission have opened new opportunities for Imaging living cells and in vivo animal models at unprecedented sensitivity and spatial resolution. Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, bioconjugated quantum dot probes are well suited for Imaging single-molecule dynamics in living cells, for monitoring protein–protein interactions within specific intraCellular locations, and for detecting diseased sites and organs in deep tissue. In this article, we describe the engineering principles for preparing high-quality quantum dots and for conjugating the dots to biomolecular ligands. We also discuss recent advances in using quantum dots for in vivo molecular and Cellular Imaging.

  • Chemical analysis and Cellular Imaging with quantum dots
    The Analyst, 2004
    Co-Authors: Andrew M. Smith, Shuming Nie
    Abstract:

    Quantum dots are tiny light-emitting particles on the nanometer scale. They are emerging as a new class of biological labels with properties and applications that are not available with traditional organic dyes and fluorescent proteins. Their novel properties such as improved brightness, resistance against photobleaching, and multicolor light emission, have opened new possibilities for ultrasensitive chemical analysis and Cellular Imaging. In this Research Highlight article, we discuss the unique optical properties of semiconductor quantum dots, surface chemistry and bioconjugation, current applications in bioanalytical chemistry and cell biology, and future research directions.

  • Quantum dot nanocrystals for in vivo molecular and Cellular Imaging
    Photochemistry and photobiology, 2004
    Co-Authors: Andrew M. Smith, Xiaohu Gao, Shuming Nie
    Abstract:

    Semiconductor quantum dots (QD) are nanometer-sized crystals with unique photochemical and photophysical properties that are not available from either isolated molecules or bulk solids. In comparison with organic dyes and fluorescent proteins, QD are emerging as a new class of fluorescent labels with improved brightness, resistance against photobleaching and multicolor fluorescence emission. These properties could improve the sensitivity of biological detection and Imaging by at least 10- to 100-fold. Further development in high-quality near-infrared-emitting QD should allow ultrasensitive and multicolor Imaging of molecular targets in deep tissue and living animals. Here, we discuss recent developments in QD synthesis and bioconjugation, applications in molecular and Cellular Imaging as well as promising directions for future research.

  • Quantum Dots as Fluorescent Labels for Molecular and Cellular Imaging
    Reviews in Fluorescence 2006, 1
    Co-Authors: Gang Ruan, Andrew M. Smith, Amit Agrawal, Xiaohu Gao, Shuming Nie
    Abstract:

    Quantum dots (QDs) are light-emitting particles on the nanometer scale, with novel optical and electronic properties that are not available from either isolated molecules or bulk solids. They are under intense development for a broad range of biological applications, including single molecule biophysics, biomolecular profiling, optical barcoding, molecular and Cellular Imaging. In comparison with organic dyes and fluorescent proteins, semiconductor QDs offers several unique advantages such as sizeand composition-tunable emission from visible to infrared wavelengths, large absorption coefficients across a wide spectral range, and very high levels of brightness and photostabilityV Due to their broad excitation profiles and narrow/symmetric emission spectra, high-quality QDs are also well suited for combinatorial optical encoding, in which multiple colors and intensities are combined to encode thousands of genes, proteins, or small-molecule compounds^'^. Despite their relatively large sizes (2-8 nm diameter), recent research has shown that bioconjugated QD probes behave like fluorescent proteins (4-6 nm), and do not suffer from serious binding kinetic or steric-hindrance problems^"^ . In this "mesoscopic" size range, QDs also have more surface areas and functionalities that can be used for linking to multiple diagnostic (e.g., radioisotopic or magnetic) and therapeutic (e.g., anticancer) agents. Furthermore, polymer-encapsulated QDs have been found to be essentially nontoxic to cells and animal models. This review will focus on the development and applications of QDs for molecular and Cellular Imaging. We

Yuan Zhi Liu - One of the best experts on this subject based on the ideXlab platform.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging.
    Biomedical optics express, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Andrew J. Bower, P. Scott Carney, Stephen A. Boppart
    Abstract:

    Three-dimensional high-resolution Imaging methods are important for Cellular-level research. Optical coherence microscopy (OCM) is a low-coherence-based interferometry technology for Cellular Imaging with both high axial and lateral resolution. Using a high-numerical-aperture objective, OCM normally has a shallow depth of field and requires scanning the focus through the entire region of interest to perform volumetric Imaging. With a higher-numerical-aperture objective, the image quality of OCM is affected by and more sensitive to aberrations. Interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) are computed Imaging techniques that overcome the depth-of-field limitation and the effect of optical aberrations in optical coherence tomography (OCT), respectively. In this work we combine OCM with ISAM and CAO to achieve high-speed volumetric Cellular Imaging. Experimental Imaging results of ex vivo human breast tissue, ex vivo mouse brain tissue, in vitro fibroblast cells in 3D scaffolds, and in vivo human skin demonstrate the significant potential of this technique for high-speed volumetric Cellular Imaging.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging
    Biomedical Optics 2014, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Paul Scott Carney, Stephen A. Boppart
    Abstract:

    We demonstrate the combination of interferometric synthetic aperture microscopy (ISAM), computational adaptive optics (CAO) and coherence tomography microscopy (OCM) provides a high-speed volumetric Cellular Imaging.

Andrew M. Smith - One of the best experts on this subject based on the ideXlab platform.

  • bioconjugated quantum dots for in vivo molecular and Cellular Imaging
    Advanced Drug Delivery Reviews, 2008
    Co-Authors: Andrew M. Smith, Hongwei Duan, Aaron M Mohs
    Abstract:

    Semiconductor quantum dots (QDs) are tiny light-emitting particles on the nanometer scale, and are emerging as a new class of fluorescent labels for biology and medicine. In comparison with organic dyes and fluorescent proteins, they have unique optical and electronic properties, with size-tunable light emission, superior signal brightness, resistance to photobleaching, and broad absorption spectra for simultaneous excitation of multiple fluorescence colors. QDs also provide a versatile nanoscale scaffold for designing multifunctional nanoparticles with both Imaging and therapeutic functions. When linked with targeting ligands such as antibodies, peptides or small molecules, QDs can be used to target tumor biomarkers as well as tumor vasculatures with high affinity and specificity. Here we discuss the synthesis and development of state-of-the-art QD probes and their use for molecular and Cellular Imaging. We also examine key issues for in vivo Imaging and therapy, such as nanoparticle biodistribution, pharmacokinetics, and toxicology.

  • Engineering Luminescent Quantum Dots for In Vivo Molecular and Cellular Imaging
    Annals of Biomedical Engineering, 2006
    Co-Authors: Andrew M. Smith, Gang Ruan, Matthew N. Rhyner, Shuming Nie
    Abstract:

    Semiconductor quantum dots are luminescent nanoparticles that are under intensive development for use as a new class of optical Imaging contrast agents. Their novel properties such as optical tunability, improved photostability, and multicolor light emission have opened new opportunities for Imaging living cells and in vivo animal models at unprecedented sensitivity and spatial resolution. Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, bioconjugated quantum dot probes are well suited for Imaging single-molecule dynamics in living cells, for monitoring protein–protein interactions within specific intraCellular locations, and for detecting diseased sites and organs in deep tissue. In this article, we describe the engineering principles for preparing high-quality quantum dots and for conjugating the dots to biomolecular ligands. We also discuss recent advances in using quantum dots for in vivo molecular and Cellular Imaging.

  • Chemical analysis and Cellular Imaging with quantum dots
    The Analyst, 2004
    Co-Authors: Andrew M. Smith, Shuming Nie
    Abstract:

    Quantum dots are tiny light-emitting particles on the nanometer scale. They are emerging as a new class of biological labels with properties and applications that are not available with traditional organic dyes and fluorescent proteins. Their novel properties such as improved brightness, resistance against photobleaching, and multicolor light emission, have opened new possibilities for ultrasensitive chemical analysis and Cellular Imaging. In this Research Highlight article, we discuss the unique optical properties of semiconductor quantum dots, surface chemistry and bioconjugation, current applications in bioanalytical chemistry and cell biology, and future research directions.

  • Quantum dot nanocrystals for in vivo molecular and Cellular Imaging
    Photochemistry and photobiology, 2004
    Co-Authors: Andrew M. Smith, Xiaohu Gao, Shuming Nie
    Abstract:

    Semiconductor quantum dots (QD) are nanometer-sized crystals with unique photochemical and photophysical properties that are not available from either isolated molecules or bulk solids. In comparison with organic dyes and fluorescent proteins, QD are emerging as a new class of fluorescent labels with improved brightness, resistance against photobleaching and multicolor fluorescence emission. These properties could improve the sensitivity of biological detection and Imaging by at least 10- to 100-fold. Further development in high-quality near-infrared-emitting QD should allow ultrasensitive and multicolor Imaging of molecular targets in deep tissue and living animals. Here, we discuss recent developments in QD synthesis and bioconjugation, applications in molecular and Cellular Imaging as well as promising directions for future research.

  • Quantum Dots as Fluorescent Labels for Molecular and Cellular Imaging
    Reviews in Fluorescence 2006, 1
    Co-Authors: Gang Ruan, Andrew M. Smith, Amit Agrawal, Xiaohu Gao, Shuming Nie
    Abstract:

    Quantum dots (QDs) are light-emitting particles on the nanometer scale, with novel optical and electronic properties that are not available from either isolated molecules or bulk solids. They are under intense development for a broad range of biological applications, including single molecule biophysics, biomolecular profiling, optical barcoding, molecular and Cellular Imaging. In comparison with organic dyes and fluorescent proteins, semiconductor QDs offers several unique advantages such as sizeand composition-tunable emission from visible to infrared wavelengths, large absorption coefficients across a wide spectral range, and very high levels of brightness and photostabilityV Due to their broad excitation profiles and narrow/symmetric emission spectra, high-quality QDs are also well suited for combinatorial optical encoding, in which multiple colors and intensities are combined to encode thousands of genes, proteins, or small-molecule compounds^'^. Despite their relatively large sizes (2-8 nm diameter), recent research has shown that bioconjugated QD probes behave like fluorescent proteins (4-6 nm), and do not suffer from serious binding kinetic or steric-hindrance problems^"^ . In this "mesoscopic" size range, QDs also have more surface areas and functionalities that can be used for linking to multiple diagnostic (e.g., radioisotopic or magnetic) and therapeutic (e.g., anticancer) agents. Furthermore, polymer-encapsulated QDs have been found to be essentially nontoxic to cells and animal models. This review will focus on the development and applications of QDs for molecular and Cellular Imaging. We

Nathan D. Shemonski - One of the best experts on this subject based on the ideXlab platform.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging.
    Biomedical optics express, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Andrew J. Bower, P. Scott Carney, Stephen A. Boppart
    Abstract:

    Three-dimensional high-resolution Imaging methods are important for Cellular-level research. Optical coherence microscopy (OCM) is a low-coherence-based interferometry technology for Cellular Imaging with both high axial and lateral resolution. Using a high-numerical-aperture objective, OCM normally has a shallow depth of field and requires scanning the focus through the entire region of interest to perform volumetric Imaging. With a higher-numerical-aperture objective, the image quality of OCM is affected by and more sensitive to aberrations. Interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) are computed Imaging techniques that overcome the depth-of-field limitation and the effect of optical aberrations in optical coherence tomography (OCT), respectively. In this work we combine OCM with ISAM and CAO to achieve high-speed volumetric Cellular Imaging. Experimental Imaging results of ex vivo human breast tissue, ex vivo mouse brain tissue, in vitro fibroblast cells in 3D scaffolds, and in vivo human skin demonstrate the significant potential of this technique for high-speed volumetric Cellular Imaging.

  • Computed optical interferometric tomography for high-speed volumetric Cellular Imaging
    Biomedical Optics 2014, 2014
    Co-Authors: Yuan Zhi Liu, Nathan D. Shemonski, Steven G. Adie, Adeel Ahmad, Paul Scott Carney, Stephen A. Boppart
    Abstract:

    We demonstrate the combination of interferometric synthetic aperture microscopy (ISAM), computational adaptive optics (CAO) and coherence tomography microscopy (OCM) provides a high-speed volumetric Cellular Imaging.