The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Guangming Zeng - One of the best experts on this subject based on the ideXlab platform.
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biotransformation of cadmium sulfamethazine combined pollutant in aqueous environments phanerochaete Chrysosporium bring cautious optimism
Chemical Engineering Journal, 2018Co-Authors: Xueying Guo, Guangming Zeng, Danlian Huang, Min Cheng, Zhiwei Peng, Shuang Zhou, Xiaomin Gong, Rui Deng, Hao Luo, Xuelei YanAbstract:Abstract Microorganism biotransformation of sulfamethazine (SMT) in aqueous environments is a major concern, especially considering their exposure to coexisting SMT and heavy metals. Phanerochaete Chrysosporium (P. Chrysosporium) is a more concerned Cadmium (Cd) and SMT hyper accumulation specie. This study, referring to metabolic mechanisms and application, was performed to investigate the single and combined effects of Cd-SMT, including toxicity, resistance, as well as the accumulation and biotransformation by P. Chrysosporium. The results revealed that Cd-SMT co-contamination caused increasing active oxygen accumulation, and the number of antioxidant enzyme and non-enzymatic antioxidants were higher than that under the stress of their individual pollution. It was found that P. Chrysosporium accumulated high levels of Cd with the increment of 6.98–23.96% induced by Cd-SMT co-contamination compared to under the stress of Cd individual pollution. What's more, the addition of Cd reduced the toxicity of SMT to P. Chrysosporium. The decrease of malonaldehyde and the increase of protein also proved that P. Chrysosporium held enormous potential to fit in the co-contaminated environment, and to remediate the co-contaminated water especially in the long-term treatment. These results undoubtedly contribute to the development of fungi-based technologies and the applications of P. Chrysosporium in realistic environment rather than laboratory simulation environment.
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the combination of fenton process and phanerochaete Chrysosporium for the removal of bisphenol a in river sediments mechanism related to extracellular enzyme organic acid and iron
Chemical Engineering Journal, 2018Co-Authors: Chanjuan Hu, Guangming Zeng, Danlian Huang, Min Cheng, Xiaomin Gong, Rongzhong Wang, Zhengxun HuAbstract:Abstract In this study, Fenton process combined with bioremediation technology, as a novel treatment technology, was applied for the removal of bisphenol A (BPA) from river sediments. The removal rate of BPA reached 58.23% after 24 days of combined treatment, which was higher than those in the treatment with Phanerochaete Chrysosporium (P. Chrysosporium) alone (21.59%) or the Fenton treatment alone (14.48%). The degradation mechanism of BPA in treatment process was investigated. According to the analyses of pH, iron, enzyme activities and organic acids, it was found that there was a synergetic effect between Fenton process and P. Chrysosporium treatment. The organic acids generated by P. Chrysosporium created a better acid environment for Fenton reaction, and the ferrous iron introduced by Fenton reagents might stimulate the development of P. Chrysosporium. In addition, β-cyclodextrin (β-CD) is a good chelating agent together with excellent bioavailability, so we investigated the influence of β-CD on the combination treatment. Results showed that β-CD was able to promote the combination treatment. We also obtained that the optimal dosage of FeSO4 (270 mmol/L), ratio of Fe2+ to H2O2 and dosage of β-CD (13 mmol/L) were 0.5 mL, 1:14 (mol/mol), 1 mL, respectively. The combined treatment under mild reaction conditions provides a new way for the removal of BPA from polluted river sediments.
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lead induced oxidative stress and antioxidant response provide insight into the tolerance of phanerochaete Chrysosporium to lead exposure
Chemosphere, 2017Co-Authors: Chao Huang, Guangming Zeng, Danlian Huang, Cui Lai, Min Cheng, Jia Wan, Jiachao Zhang, Chen Zhang, Rongzhong WangAbstract:Abstract The present work investigated the effect of lead (Pb) on the growth, metal accumulation, oxidative stress, and antioxidant response in Phanerochaete Chrysosporium , which is a well-known hyperaccumulating species for heavy metal with appreciable bioaccumulation capacity. Results revealed that P. Chrysosporium exhibited a good ability in Pb accumulation and tolerance over a concentration range of 50–100 mg L −1 Pb. The removal rate of Pb decreased with the increasing levels of Pb and reached a maximum of 91.3% at 50 mg L −1 . Both extracellular adsorption and intracellular bioaccumulation contributed to the removal of Pb, with the maximum of 123.8 mg g −1 and 162.5 mg g −1 dry weight, respectively. Pb may exert its toxicity to P. Chrysosporium by impairing oxidative metabolism, as evidenced by the enhanced accumulation of hydrogen peroxide (H 2 O 2 ) and lipid peroxidation product malonaldehyde (MDA). P. Chrysosporium evolved an antioxidant system by elevating the activity of superoxide dismutase (SOD) and the level of reduced glutathione (GSH) in response to Pb stress, whereas decreasing the activities of catalase (CAT) and peroxidase (POD). Moreover, Pearson correlation analysis demonstrated a good correlation between oxidative stress biomarkers and enzymatic antioxidants. The preset work suggested that P. Chrysosporium exhibited an outstanding accumulation of Pb and tolerance of Pb-induced oxidative stress by the effective antioxidant defense mechanism.
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comprehensive evaluation of the cytotoxicity of cdse zns quantum dots in phanerochaete Chrysosporium by cellular uptake and oxidative stress
Environmental science. Nano, 2017Co-Authors: Jia Wan, Guangming Zeng, Min Cheng, Anwei Chen, Guiqiu Chen, Zhenzhen Huang, Chengyun Zhou, Weiping Xiong, Cui LaiAbstract:The growing potential of quantum dots (QDs) in biological and biomedical applications has raised considerable concern due to their toxicological impact. Consequently, it is urgent to elucidate the underlying toxicity mechanism of QDs. In this work, we comprehensively investigated the cellular uptake of four CdSe/ZnS QDs (COOH CdSe/ZnS 525, COOH CdSe/ZnS 625, NH2 CdSe/ZnS 525, and NH2 CdSe/ZnS 625) and induced physiological responses in Phanerochaete Chrysosporium (P. Chrysosporium) through inductively coupled plasma optical emission spectroscopy, confocal laser scanning microscopy, and the determination of malondialdehyde content, superoxide level, superoxide dismutase activity, catalase activity and glutathione level. The results showed that the four CdSe/ZnS QDs accumulated largely in the hyphae and caused oxidative stress to P. Chrysosporium in the tested concentration range (10–80 nM). Furthermore, the cellular uptake and cytotoxicity were related to the physicochemical properties of the QDs, such as particle size and surface charges. Negatively charged CdSe/ZnS QDs with small size could be more easily ingested by P. Chrysosporium than large ones; thus small size CdSe/ZnS QDs were more cytotoxic to P. Chrysosporium. On the other hand, small negatively charged CdSe/ZnS QDs resulted in greater cytotoxicity than large negatively charged CdSe/ZnS QDs. The obtained results offer valuable information for revealing the toxicity mechanism of QDs in living cells.
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Toxicity and bioaccumulation of heavy metals in Phanerochaete Chrysosporium
Transactions of Nonferrous Metals Society of China, 2016Co-Authors: Meihua Zhao, Guangming Zeng, Danlian Huang, Chaosheng Zhang, Min ChengAbstract:Abstract The responses of the growth and metabolism activity of Phanerochaete Chrysosporium ( P. Chrysosporium ) to cadmium (Cd), lead (Pb) and their combined pollution stress, were investigated in plate and liquid culture conditions. The diameter of colony, biomass of P. Chrysosporium , ligninolytic enzyme activities and bioaccumulation quantity of heavy metals were detected. The results indicated that Cd was more toxic than Pb to P. Chrysosporium and the toxicity of Cd and Pb to P. Chrysosporium was further strengthened under Cd+Pb combined pollution in different culture conditions. Heavy metals Cd and Pb had indirect influence on the production of ligninolytic enzymes by directly affecting the fungal growth and metabolic activity, and by another way in liquid culture. In addition, the results provided an evidence of the accumulation of Cd and Pb on the mycelia of P. Chrysosporium .
Danlian Huang - One of the best experts on this subject based on the ideXlab platform.
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biotransformation of cadmium sulfamethazine combined pollutant in aqueous environments phanerochaete Chrysosporium bring cautious optimism
Chemical Engineering Journal, 2018Co-Authors: Xueying Guo, Guangming Zeng, Danlian Huang, Min Cheng, Zhiwei Peng, Shuang Zhou, Xiaomin Gong, Rui Deng, Hao Luo, Xuelei YanAbstract:Abstract Microorganism biotransformation of sulfamethazine (SMT) in aqueous environments is a major concern, especially considering their exposure to coexisting SMT and heavy metals. Phanerochaete Chrysosporium (P. Chrysosporium) is a more concerned Cadmium (Cd) and SMT hyper accumulation specie. This study, referring to metabolic mechanisms and application, was performed to investigate the single and combined effects of Cd-SMT, including toxicity, resistance, as well as the accumulation and biotransformation by P. Chrysosporium. The results revealed that Cd-SMT co-contamination caused increasing active oxygen accumulation, and the number of antioxidant enzyme and non-enzymatic antioxidants were higher than that under the stress of their individual pollution. It was found that P. Chrysosporium accumulated high levels of Cd with the increment of 6.98–23.96% induced by Cd-SMT co-contamination compared to under the stress of Cd individual pollution. What's more, the addition of Cd reduced the toxicity of SMT to P. Chrysosporium. The decrease of malonaldehyde and the increase of protein also proved that P. Chrysosporium held enormous potential to fit in the co-contaminated environment, and to remediate the co-contaminated water especially in the long-term treatment. These results undoubtedly contribute to the development of fungi-based technologies and the applications of P. Chrysosporium in realistic environment rather than laboratory simulation environment.
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the combination of fenton process and phanerochaete Chrysosporium for the removal of bisphenol a in river sediments mechanism related to extracellular enzyme organic acid and iron
Chemical Engineering Journal, 2018Co-Authors: Chanjuan Hu, Guangming Zeng, Danlian Huang, Min Cheng, Xiaomin Gong, Rongzhong Wang, Zhengxun HuAbstract:Abstract In this study, Fenton process combined with bioremediation technology, as a novel treatment technology, was applied for the removal of bisphenol A (BPA) from river sediments. The removal rate of BPA reached 58.23% after 24 days of combined treatment, which was higher than those in the treatment with Phanerochaete Chrysosporium (P. Chrysosporium) alone (21.59%) or the Fenton treatment alone (14.48%). The degradation mechanism of BPA in treatment process was investigated. According to the analyses of pH, iron, enzyme activities and organic acids, it was found that there was a synergetic effect between Fenton process and P. Chrysosporium treatment. The organic acids generated by P. Chrysosporium created a better acid environment for Fenton reaction, and the ferrous iron introduced by Fenton reagents might stimulate the development of P. Chrysosporium. In addition, β-cyclodextrin (β-CD) is a good chelating agent together with excellent bioavailability, so we investigated the influence of β-CD on the combination treatment. Results showed that β-CD was able to promote the combination treatment. We also obtained that the optimal dosage of FeSO4 (270 mmol/L), ratio of Fe2+ to H2O2 and dosage of β-CD (13 mmol/L) were 0.5 mL, 1:14 (mol/mol), 1 mL, respectively. The combined treatment under mild reaction conditions provides a new way for the removal of BPA from polluted river sediments.
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lead induced oxidative stress and antioxidant response provide insight into the tolerance of phanerochaete Chrysosporium to lead exposure
Chemosphere, 2017Co-Authors: Chao Huang, Guangming Zeng, Danlian Huang, Cui Lai, Min Cheng, Jia Wan, Jiachao Zhang, Chen Zhang, Rongzhong WangAbstract:Abstract The present work investigated the effect of lead (Pb) on the growth, metal accumulation, oxidative stress, and antioxidant response in Phanerochaete Chrysosporium , which is a well-known hyperaccumulating species for heavy metal with appreciable bioaccumulation capacity. Results revealed that P. Chrysosporium exhibited a good ability in Pb accumulation and tolerance over a concentration range of 50–100 mg L −1 Pb. The removal rate of Pb decreased with the increasing levels of Pb and reached a maximum of 91.3% at 50 mg L −1 . Both extracellular adsorption and intracellular bioaccumulation contributed to the removal of Pb, with the maximum of 123.8 mg g −1 and 162.5 mg g −1 dry weight, respectively. Pb may exert its toxicity to P. Chrysosporium by impairing oxidative metabolism, as evidenced by the enhanced accumulation of hydrogen peroxide (H 2 O 2 ) and lipid peroxidation product malonaldehyde (MDA). P. Chrysosporium evolved an antioxidant system by elevating the activity of superoxide dismutase (SOD) and the level of reduced glutathione (GSH) in response to Pb stress, whereas decreasing the activities of catalase (CAT) and peroxidase (POD). Moreover, Pearson correlation analysis demonstrated a good correlation between oxidative stress biomarkers and enzymatic antioxidants. The preset work suggested that P. Chrysosporium exhibited an outstanding accumulation of Pb and tolerance of Pb-induced oxidative stress by the effective antioxidant defense mechanism.
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Toxicity and bioaccumulation of heavy metals in Phanerochaete Chrysosporium
Transactions of Nonferrous Metals Society of China, 2016Co-Authors: Meihua Zhao, Guangming Zeng, Danlian Huang, Chaosheng Zhang, Min ChengAbstract:Abstract The responses of the growth and metabolism activity of Phanerochaete Chrysosporium ( P. Chrysosporium ) to cadmium (Cd), lead (Pb) and their combined pollution stress, were investigated in plate and liquid culture conditions. The diameter of colony, biomass of P. Chrysosporium , ligninolytic enzyme activities and bioaccumulation quantity of heavy metals were detected. The results indicated that Cd was more toxic than Pb to P. Chrysosporium and the toxicity of Cd and Pb to P. Chrysosporium was further strengthened under Cd+Pb combined pollution in different culture conditions. Heavy metals Cd and Pb had indirect influence on the production of ligninolytic enzymes by directly affecting the fungal growth and metabolic activity, and by another way in liquid culture. In addition, the results provided an evidence of the accumulation of Cd and Pb on the mycelia of P. Chrysosporium .
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The Oxidative Stress of Phanerochaete Chrysosporium Against Lead Toxicity
Applied biochemistry and biotechnology, 2014Co-Authors: Jia Wan, Guangming Zeng, Danlian Huang, Cui Lai, Chao Huang, Zhen Wei, Mingyong LaiAbstract:Among the technologies for heavy metal remediation, bioremediation technology has gained extensive attention because of its low processing costs and high efficiency. The white-rot fungus Phanerochaete Chrysosporium (P. Chrysosporium) which has a good tolerance to heavy metals has been widely used in the heavy metal bioremediation. In order to figure out the molecular mechanisms involved in the oxidative stress of P. Chrysosporium against metal toxicity, we examined the effect of Pb2+ on the levels of reactive oxygen species and the production of malondialdehyde. Results showed that P. Chrysosporium could adjust Pb-stressed condition by regulating the unique oxidation-antioxidation process in cells and kept a balance between oxidation and antioxidation when it was threatened by a different dose of Pb2+. Investigations into the oxidative stress of P. Chrysosporium to lead could not only provide a better understanding of the relationship between lead and oxidative stress in P. Chrysosporium, but also offer important informations on the development of fungal-based remediation technologies to reduce the toxic effects of lead.
Hongli Yuan - One of the best experts on this subject based on the ideXlab platform.
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Comparative characterization of extracellular enzymes secreted by Phanerochaete Chrysosporium during solid-state and submerged fermentation.
International journal of biological macromolecules, 2020Co-Authors: Jiawen Liu, Jinshui Yang, Ruonan Wang, Liang Liu, Yu Zhang, Huifang Bao, Jin Myong Jang, En Tao Wang, Hongli YuanAbstract:Influence of water content on the expression of lignocellulolytic enzymes by Phanerochaete Chrysosporium remains unclear. This work compares the enzyme production profiles of P. Chrysosporium during solid-state and submerged fermentation. There were 110 and 64 extracellular carbohydrate-active enzymes identified in solid-state and submerged fermentation respectively, among which 57 enzymes were common to both of the secretomes. P. Chrysosporium secreted more cellulases (especially lytic polysaccharide monooxygenase) and hemicellulases during solid-state fermentation while the proportion of enzyme containing carbohydrate-binding module was higher for submerged fermentation. Although its activities were weaker, the enzyme cocktail from submerged fermentation was surprisingly more effective in hydrolysis at low substrate loading. This advantage of enzymes from submerged fermentation was mainly attributed to carbohydrate-binding module because more xylanases bound with substrate at the beginning of hydrolysis. These results reveal the influence of fermentation conditions on enzyme produced by P. Chrysosporium for the first time and show the importance of carbohydrate-binding module in the hydrolysis process of lignocellulose.
Lin Tang - One of the best experts on this subject based on the ideXlab platform.
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influence of phanerochaete Chrysosporium on microbial communities and lignocellulose degradation during solid state fermentation of rice straw
Process Biochemistry, 2009Co-Authors: Guangming Zeng, Danlian Huang, Yaoning Chen, Lin TangAbstract:Abstract To investigate the changes of microbial communities and influence of Phanerochaete Chrysosporium during solid-state fermentation (SSF) of rice straw, phospholipid fatty acids (PLFA) and lignocellulose components were measured with periodical sampling. The results showed that the lignocellulose degrading ratios in SSF which was inoculated by P. Chrysosporium and soil microorganisms were higher than those degraded by culturing a single species. The total amount of PLFAs, as an indicator of microbial biomass, reached the peak on day 6. Principal component analysis (PCA) of the PLFA compositions revealed that P. Chrysosporium was well responsible for the succession of microbial community and showed that fungi were the predominant species at the end of the process. The correlation analysis between lignocellulose degrading ratio and PLFA profile in P. Chrysosporium suggested that P. Chrysosporium promoted lignin degrading as the main fungi with gram-positive bacteria.
Sheng-tao Yang - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of carbon nanotubes to white rot fungus Phanerochaete Chrysosporium.
Ecotoxicology and environmental safety, 2018Co-Authors: Zhu Ming, Shicheng Feng, Ailimire Yilihamu, Shengnan Yang, Hua Yang, Yitong Bai, Sheng-tao YangAbstract:Carbon nanotubes (CNTs) are widely used in diverse areas with increasing annual production, thus the environmental impact of CNTs needs thorough investigation. In this study, we evaluated the effect of pristine multi-walled CNTs (p-MWCNTs) and oxidized multi-walled CNTs (o-MWCNTs) on white rot fungus Phanerochaete Chrysosporium, which is the decomposer in carbon cycle and also has many applications in environmental remediation. Both p-MWCNTs and o-MWCNTs had no influence on the dry weight increase of P. Chrysosporium and the pH value of culture system. The fibrous structure of P. Chrysosporium was disturbed by p-MWCNTs seriously, while o-MWCNTs had litter influence. The ultrastructural changes were more evident for P. Chrysosporium exposed to p-MWCNTs and only p-MWCNTs could penetrate into the cell plasma. The chemical composition of P. Chrysosporium was nearly unchanged according to the infrared spectra. The laccase activity was suppressed by p-MWCNTs, while o-MWCNTs showed stimulating effect. The decoloration of reactive brilliant red X-3B was not affected by both CNT samples. However, serious inhibition of wood degradation was observed in the p-MWCNTs exposed groups, suggesting the potential threat of CNTs to the decomposition of carbon cycle. The implication to the environmental risks and safe applications of carbon nanomaterials is discussed.
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Toxicity of Pristine and Chemically Functionalized Fullerenes to White Rot Fungus Phanerochaete Chrysosporium
MDPI AG, 2018Co-Authors: Zhu Ming, Shicheng Feng, Ailimire Yilihamu, Shengnan Yang, Sheng-tao YangAbstract:Fullerenes are widely produced and applied carbon nanomaterials that require a thorough investigation into their environmental hazards and risks. In this study, we compared the toxicity of pristine fullerene (C60) and carboxylated fullerene (C60-COOH) to white rot fungus Phanerochaete Chrysosporium. The influence of fullerene on the weight increase, fibrous structure, ultrastructure, enzyme activity, and decomposition capability of P. Chrysosporium was investigated to reflect the potential toxicity of fullerene. C60 did not change the fresh and dry weights of P. Chrysosporium but C60-COOH inhibited the weight gain at high concentrations. Both C60 and C60-COOH destroyed the fibrous structure of the mycelia. The ultrastructure of P. Chrysosporium was changed by C60-COOH. Pristine C60 did not affect the enzyme activity of the P. Chrysosporium culture system while C60-COOH completely blocked the enzyme activity. Consequently, in the liquid culture, P. Chrysosporium lost the decomposition activity at high C60-COOH concentrations. The decreased capability in degrading wood was observed for P. Chrysosporium exposed to C60-COOH. Our results collectively indicate that chemical functionalization enhanced the toxicity of fullerene to white rot fungi and induced the loss of decomposition activity. The environmental risks of fullerene and its disturbance to the carbon cycle are discussed
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toxicity of graphene oxide to white rot fungus phanerochaete Chrysosporium
Chemosphere, 2016Co-Authors: Zhu Ming, Hongliang Li, Hua Yang, Baowei Yu, Ruihan Wu, Sheng-tao YangAbstract:Abstract With the wide production and applications of graphene and its derivatives, their toxicity to the environment has received much attention nowadays. In this study, we investigated the toxicity of graphene oxide (GO) to white rot fungus (Phanerochaete Chrysosporium). GO was prepared by modified Hummers method and well characterized before use. P. Chrysosporium was exposed to GO at the concentrations of 0–4 mg/mL for 7 d. The fresh and dry weights, pH values of culture media, structures, ultrastructures, IR spectra and activities of the decomposition of pollutants were measured to reveal the hazards of GO to P. Chrysosporium. Our results indicated that low concentrations of GO stimulated the growth of P. Chrysosporium. The exposure to GO induced more acidic pH values of the culture media after 7 d. GO induced the disruption of the fiber structure of P. Chrysosporium, while at 4 mg/mL some very long and thick fibers were formed. Such changes were reflected in the scanning electron microscopy investigations, where the disruption of fibers was observed. In the ultrastructural investigations, the shape of P. Chrysosporium cells changed and more vesicles were found upon the exposure to GO. The infrared spectroscopy analyses suggested that the chemical compositions of mycelia were not changed qualitatively. Beyond the toxicity, GO did not alter the activities of P. Chrysosporium at low concentrations, but led to the complete loss of activity at high concentrations. The implication to the ecological safety of graphene is discussed.