The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Michael Zuykov - One of the best experts on this subject based on the ideXlab platform.
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Pre-exposure to Cu2+ and CuO NPs leads to infection of caged blue mussels, Mytilus edulis L., by pathogenic microalga: Pilot study in the Lower St. Lawrence Estuary (Québec, Canada).
Marine pollution bulletin, 2021Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Galina Kolyuchkina, Graeme Spiers, Michael SchindlerAbstract:As evidenced from literature, exposure to non-lethal concentrations of dissolved copper (Cu2+) and copper nanoparticles (CuO NPs) promotes blue mussels susceptibility to various bacterial infections. We study whether pre-exposure (3.5 h) with CuSO4 (100 μg Cu L-1) and CuO NPs (1000 μg Cu L-1) will result in infection of M. edulis L. with pathogenic microalga Coccomyxa sp. under field conditions. In May - September 2019, cages were installed in the site Metis-sur-Mer, St. Lawrence Estuary (QC, Canada) where the native mussel population is known to be infected with the pathogen. Untreated and pre-exposed mussels were grown for up to 130 days. Only the mussels pre-exposed to copper were infected by Coccomyxa. This finding allows proposing that occurrences of Coccomyxa-infected mussels worldwide might have an association with water pollution with xenobiotics. Pre-exposure of caged mussels to copper, as a protocol monitoring for other infectious agents, can be recommended to test.
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First report of signs of infection by Coccomyxa-like algae in wild blue mussels, Mytilus spp., in the Gulf of Maine (USA, Maine)
Journal of fish diseases, 2020Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Graeme Spiers, Bassem Allam, Michael SchindlerAbstract:In August 2019, visual inspection of intertidal zones of the Gulf of Maine (ME, USA) revealed young and adult wild blue mussels, Mytilus spp., in Alley Bay (Jonesport area) with the distinctive L-shaped shell deformity (LSSD) and green spots (GS) in the mantle and adductor muscle. LSSD is a characteristic sign of current or previous mussel infection by photosynthetic unicellular alga from the group Coccomyxa, while GS are algal colonies. Based on these findings, this study represents the first report of infection signs by pathogenic Coccomyxa-like algae in mussels from the coastal waters of the Northeastern United States, providing a base for future large scale monitoring of the alga in the region.
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Shell deformity as a marker for retrospective detection of a pathogenic unicellular alga, Coccomyxa sp., in mytilid mussels: A first case study and research agenda.
Journal of invertebrate pathology, 2019Co-Authors: Michael Zuykov, Michel Gosselin, Julia Anderson, Philippe Archambault, Graeme Spiers, G. A. Kolyuchkina, Christopher W. Mckindsey, Michael SchindlerAbstract:Abstract An L-shaped shell deformity (LSSD) on the posterior shell edge is known exclusively in wild mytilid mussels infected with photosynthetic Coccomyxa-like algae. LSSD forms due to the appearance of extra shell material; it only occurs if the mussel is heavily infected with the alga. Traditionally, observation of high amount of the green spots (algal colonies) on a large area of host soft tissues (most of the mantle and in adductor muscle) has been used to indicate a high infection rate. We examined 300 Mytilus spp. (100 small, 20–30 mm; 200 large, 40–60 mm) with a high degree of LSSD (parameter “d” > 5 mm) from the Lower St. Lawrence Estuary (Quebec, Canada). Green spots were absent in two large mussels, and were only present along the mantle posterior edge in 14 large mussels; other individuals had high infection levels. Our observations suggest that some individuals could be in a state of remission, or, even more optimistically - mussels may be able to resist the pathogen. LSSD is the stable through-time marker for detection of mytilid mussels that are or were infected with Coccomyxa algae, and, thus, may provide information for the study of mussel immunity and control of alga distribution/migration in coastal waters worldwide.
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does photosynthesis provoke formation of shell deformity in wild mytilid mussels infested with green microalgae Coccomyxa a conceptual model and research agenda
Journal of Experimental Marine Biology and Ecology, 2018Co-Authors: Michael Zuykov, Julia Anderson, Émilien PelletierAbstract:Abstract This report proposes a conceptual model for the formation of L-shaped shell deformity in wild mytilid mussels Mytilus spp. highly infested by unicellular photosynthetic microalgae Coccomyxa sp. where a key role plays the influence of the alga's photosynthesis on the parameters of carbonate system at the site of calcification. A number of research questions are posed, to be investigated through a combination of experimental methods.
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Mytilus trossulus and hybrid (M. edulis-M. trossulus) - New hosts organisms for pathogenic microalgae Coccomyxa sp. from the Estuary and northwestern Gulf of St. Lawrence, Canada.
Journal of invertebrate pathology, 2018Co-Authors: Michael Zuykov, Julia Anderson, Philippe Archambault, Émilien PelletierAbstract:Abstract During summer 2014–2017, wild mytilid mussels, highly infested with the pathogenic Coccomyxa-like microalgae, were collected along the Estuary and northwestern part of Gulf of St. Lawrence (Quebec, Canada). Molecular identification showed that algae can be assigned to a single taxon, Coccomyxa sp. (KJ372210), whereas hosts are represented by Mytilus edulis, M. trossulus and hybrid between these two species. This is the first record of M. trossulus and hybrid among hosts of this pathogenic alga. Our results are indicative of a possible widespread distribution of Coccomyxa sp. in the Lower St. Lawrence Estuary and along coastal waters of Canadian Maritime provinces.
Michel Gosselin - One of the best experts on this subject based on the ideXlab platform.
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Pre-exposure to Cu2+ and CuO NPs leads to infection of caged blue mussels, Mytilus edulis L., by pathogenic microalga: Pilot study in the Lower St. Lawrence Estuary (Québec, Canada).
Marine pollution bulletin, 2021Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Galina Kolyuchkina, Graeme Spiers, Michael SchindlerAbstract:As evidenced from literature, exposure to non-lethal concentrations of dissolved copper (Cu2+) and copper nanoparticles (CuO NPs) promotes blue mussels susceptibility to various bacterial infections. We study whether pre-exposure (3.5 h) with CuSO4 (100 μg Cu L-1) and CuO NPs (1000 μg Cu L-1) will result in infection of M. edulis L. with pathogenic microalga Coccomyxa sp. under field conditions. In May - September 2019, cages were installed in the site Metis-sur-Mer, St. Lawrence Estuary (QC, Canada) where the native mussel population is known to be infected with the pathogen. Untreated and pre-exposed mussels were grown for up to 130 days. Only the mussels pre-exposed to copper were infected by Coccomyxa. This finding allows proposing that occurrences of Coccomyxa-infected mussels worldwide might have an association with water pollution with xenobiotics. Pre-exposure of caged mussels to copper, as a protocol monitoring for other infectious agents, can be recommended to test.
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First report of signs of infection by Coccomyxa-like algae in wild blue mussels, Mytilus spp., in the Gulf of Maine (USA, Maine)
Journal of fish diseases, 2020Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Graeme Spiers, Bassem Allam, Michael SchindlerAbstract:In August 2019, visual inspection of intertidal zones of the Gulf of Maine (ME, USA) revealed young and adult wild blue mussels, Mytilus spp., in Alley Bay (Jonesport area) with the distinctive L-shaped shell deformity (LSSD) and green spots (GS) in the mantle and adductor muscle. LSSD is a characteristic sign of current or previous mussel infection by photosynthetic unicellular alga from the group Coccomyxa, while GS are algal colonies. Based on these findings, this study represents the first report of infection signs by pathogenic Coccomyxa-like algae in mussels from the coastal waters of the Northeastern United States, providing a base for future large scale monitoring of the alga in the region.
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Shell deformity as a marker for retrospective detection of a pathogenic unicellular alga, Coccomyxa sp., in mytilid mussels: A first case study and research agenda.
Journal of invertebrate pathology, 2019Co-Authors: Michael Zuykov, Michel Gosselin, Julia Anderson, Philippe Archambault, Graeme Spiers, G. A. Kolyuchkina, Christopher W. Mckindsey, Michael SchindlerAbstract:Abstract An L-shaped shell deformity (LSSD) on the posterior shell edge is known exclusively in wild mytilid mussels infected with photosynthetic Coccomyxa-like algae. LSSD forms due to the appearance of extra shell material; it only occurs if the mussel is heavily infected with the alga. Traditionally, observation of high amount of the green spots (algal colonies) on a large area of host soft tissues (most of the mantle and in adductor muscle) has been used to indicate a high infection rate. We examined 300 Mytilus spp. (100 small, 20–30 mm; 200 large, 40–60 mm) with a high degree of LSSD (parameter “d” > 5 mm) from the Lower St. Lawrence Estuary (Quebec, Canada). Green spots were absent in two large mussels, and were only present along the mantle posterior edge in 14 large mussels; other individuals had high infection levels. Our observations suggest that some individuals could be in a state of remission, or, even more optimistically - mussels may be able to resist the pathogen. LSSD is the stable through-time marker for detection of mytilid mussels that are or were infected with Coccomyxa algae, and, thus, may provide information for the study of mussel immunity and control of alga distribution/migration in coastal waters worldwide.
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Free-living stage of the unicellular algae Coccomyxa sp. parasite of the blue mussel (Mytilus edulis): Low-light adaptation, capacity for growth at a very wide salinity range and tolerance to low pH.
Journal of invertebrate pathology, 2015Co-Authors: Claude Belzile, Michel GosselinAbstract:Abstract Coccomyxa parasitica and similar, unidentified Coccomyxa species infect the soft tissues of many bivalve species, giving them a conspicuous green coloration and leading to mantle and shell deformities. Very little information exists regarding the ecophysiology of parasitic Coccomyxa sp. and this limits our ability to understand how it can achieve its unusual life history. In the present study, the growth of Coccomyxa sp. Metis-sur-mer (KJ372210) in liquid culture was investigated. Coccomyxa sp. maximum growth rate was 0.75 day−1 (equivalent to a doubling time of 22 h), growth saturated at ∼100 μmol quanta m−2 s−1 and was still ∼20% of maximum at 13 μmol quanta m−2 s−1, the lowest photosynthetically available radiation (PAR) intensity tested. Coccomyxa sp. showed a very wide tolerance to salinity, with growth rate practically invariable over the salinity range 15–35. Even in natural spring water enriched with f/2 media, the growth rate was nearly half of maximum. Unlike the closely related acid-tolerant Coccomyxa sp. CPCC 508, Coccomyxa sp. Metis-sur-mer could not grow in acidic waters. Considering the ability of Coccomyxa sp. to achieve a high growth rate at low irradiance and its relative insensitivity to the prevailing salinity, it is somewhat surprising it has not yet infected bivalves at a larger scale worldwide.
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First record of the green microalgae Coccomyxa sp. in blue mussel Mytilus edulis (L.) from the Lower St. Lawrence Estuary (Québec, Canada).
Journal of invertebrate pathology, 2014Co-Authors: Michael Zuykov, Claude Belzile, Michel Gosselin, Nicolas Lemaire, Émilien PelletierAbstract:Abstract During autumn 2012 and spring 2013, blue mussels Mytilus edulis (L.) with strongly deformed (L-shaped) posterior shell margins and green spots in soft tissue (microalgae) were collected from intertidal zone along the south shore of the Lower St. Lawrence Estuary near Rimouski (Quebec, Canada). Identification of algal cells infesting mussels as Coccomyxa sp. was confirmed by rRNA sequencing and HPLC pigment analysis. Flow cytometric analysis revealed the presence of algal cells in the hemolymph and extrapallial fluid in mussels with deformed and non-deformed shells; concentrations of algal cells were ranged from about 200 mL −1 in mussels with actually non-deformed shells to concentrations reaching up to 3.8 × 10 7 mL −1 in mussels with heavily deformed ones. Chemical analyses of soft tissues led us to conclude that butyltin compounds and trace metals cannot be considered among factors responsible for the shell deformity observed. Using scanning electron microscopy, the biogenic nature of the erosion on the external shell surface and aragonitic lenses of prisms in the curvature zone of deformed shells (in sections) were recorded. The sequence of the green algae from M. edulis of the Lower St. Lawrence Estuary was closely related to Coccomyxa sp. infecting M. edulis from the Flensburg Fjord (North Sea) and Modiolus modiolus (L.) from the Vityaz Bay (Sea of Japan).
Michael Schindler - One of the best experts on this subject based on the ideXlab platform.
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Pre-exposure to Cu2+ and CuO NPs leads to infection of caged blue mussels, Mytilus edulis L., by pathogenic microalga: Pilot study in the Lower St. Lawrence Estuary (Québec, Canada).
Marine pollution bulletin, 2021Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Galina Kolyuchkina, Graeme Spiers, Michael SchindlerAbstract:As evidenced from literature, exposure to non-lethal concentrations of dissolved copper (Cu2+) and copper nanoparticles (CuO NPs) promotes blue mussels susceptibility to various bacterial infections. We study whether pre-exposure (3.5 h) with CuSO4 (100 μg Cu L-1) and CuO NPs (1000 μg Cu L-1) will result in infection of M. edulis L. with pathogenic microalga Coccomyxa sp. under field conditions. In May - September 2019, cages were installed in the site Metis-sur-Mer, St. Lawrence Estuary (QC, Canada) where the native mussel population is known to be infected with the pathogen. Untreated and pre-exposed mussels were grown for up to 130 days. Only the mussels pre-exposed to copper were infected by Coccomyxa. This finding allows proposing that occurrences of Coccomyxa-infected mussels worldwide might have an association with water pollution with xenobiotics. Pre-exposure of caged mussels to copper, as a protocol monitoring for other infectious agents, can be recommended to test.
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First report of signs of infection by Coccomyxa-like algae in wild blue mussels, Mytilus spp., in the Gulf of Maine (USA, Maine)
Journal of fish diseases, 2020Co-Authors: Michael Zuykov, Michel Gosselin, Philippe Archambault, Graeme Spiers, Bassem Allam, Michael SchindlerAbstract:In August 2019, visual inspection of intertidal zones of the Gulf of Maine (ME, USA) revealed young and adult wild blue mussels, Mytilus spp., in Alley Bay (Jonesport area) with the distinctive L-shaped shell deformity (LSSD) and green spots (GS) in the mantle and adductor muscle. LSSD is a characteristic sign of current or previous mussel infection by photosynthetic unicellular alga from the group Coccomyxa, while GS are algal colonies. Based on these findings, this study represents the first report of infection signs by pathogenic Coccomyxa-like algae in mussels from the coastal waters of the Northeastern United States, providing a base for future large scale monitoring of the alga in the region.
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Shell deformity as a marker for retrospective detection of a pathogenic unicellular alga, Coccomyxa sp., in mytilid mussels: A first case study and research agenda.
Journal of invertebrate pathology, 2019Co-Authors: Michael Zuykov, Michel Gosselin, Julia Anderson, Philippe Archambault, Graeme Spiers, G. A. Kolyuchkina, Christopher W. Mckindsey, Michael SchindlerAbstract:Abstract An L-shaped shell deformity (LSSD) on the posterior shell edge is known exclusively in wild mytilid mussels infected with photosynthetic Coccomyxa-like algae. LSSD forms due to the appearance of extra shell material; it only occurs if the mussel is heavily infected with the alga. Traditionally, observation of high amount of the green spots (algal colonies) on a large area of host soft tissues (most of the mantle and in adductor muscle) has been used to indicate a high infection rate. We examined 300 Mytilus spp. (100 small, 20–30 mm; 200 large, 40–60 mm) with a high degree of LSSD (parameter “d” > 5 mm) from the Lower St. Lawrence Estuary (Quebec, Canada). Green spots were absent in two large mussels, and were only present along the mantle posterior edge in 14 large mussels; other individuals had high infection levels. Our observations suggest that some individuals could be in a state of remission, or, even more optimistically - mussels may be able to resist the pathogen. LSSD is the stable through-time marker for detection of mytilid mussels that are or were infected with Coccomyxa algae, and, thus, may provide information for the study of mussel immunity and control of alga distribution/migration in coastal waters worldwide.
Émilien Pelletier - One of the best experts on this subject based on the ideXlab platform.
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does photosynthesis provoke formation of shell deformity in wild mytilid mussels infested with green microalgae Coccomyxa a conceptual model and research agenda
Journal of Experimental Marine Biology and Ecology, 2018Co-Authors: Michael Zuykov, Julia Anderson, Émilien PelletierAbstract:Abstract This report proposes a conceptual model for the formation of L-shaped shell deformity in wild mytilid mussels Mytilus spp. highly infested by unicellular photosynthetic microalgae Coccomyxa sp. where a key role plays the influence of the alga's photosynthesis on the parameters of carbonate system at the site of calcification. A number of research questions are posed, to be investigated through a combination of experimental methods.
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Mytilus trossulus and hybrid (M. edulis-M. trossulus) - New hosts organisms for pathogenic microalgae Coccomyxa sp. from the Estuary and northwestern Gulf of St. Lawrence, Canada.
Journal of invertebrate pathology, 2018Co-Authors: Michael Zuykov, Julia Anderson, Philippe Archambault, Émilien PelletierAbstract:Abstract During summer 2014–2017, wild mytilid mussels, highly infested with the pathogenic Coccomyxa-like microalgae, were collected along the Estuary and northwestern part of Gulf of St. Lawrence (Quebec, Canada). Molecular identification showed that algae can be assigned to a single taxon, Coccomyxa sp. (KJ372210), whereas hosts are represented by Mytilus edulis, M. trossulus and hybrid between these two species. This is the first record of M. trossulus and hybrid among hosts of this pathogenic alga. Our results are indicative of a possible widespread distribution of Coccomyxa sp. in the Lower St. Lawrence Estuary and along coastal waters of Canadian Maritime provinces.
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Does photosynthesis provoke formation of shell deformity in wild mytilid mussels infested with green microalgae Coccomyxa? – A conceptual model and research agenda
Journal of Experimental Marine Biology and Ecology, 2018Co-Authors: Michael Zuykov, Julia Anderson, Émilien PelletierAbstract:Abstract This report proposes a conceptual model for the formation of L-shaped shell deformity in wild mytilid mussels Mytilus spp. highly infested by unicellular photosynthetic microalgae Coccomyxa sp. where a key role plays the influence of the alga's photosynthesis on the parameters of carbonate system at the site of calcification. A number of research questions are posed, to be investigated through a combination of experimental methods.
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First record of the green microalgae Coccomyxa sp. in blue mussel Mytilus edulis (L.) from the Lower St. Lawrence Estuary (Québec, Canada).
Journal of invertebrate pathology, 2014Co-Authors: Michael Zuykov, Claude Belzile, Michel Gosselin, Nicolas Lemaire, Émilien PelletierAbstract:Abstract During autumn 2012 and spring 2013, blue mussels Mytilus edulis (L.) with strongly deformed (L-shaped) posterior shell margins and green spots in soft tissue (microalgae) were collected from intertidal zone along the south shore of the Lower St. Lawrence Estuary near Rimouski (Quebec, Canada). Identification of algal cells infesting mussels as Coccomyxa sp. was confirmed by rRNA sequencing and HPLC pigment analysis. Flow cytometric analysis revealed the presence of algal cells in the hemolymph and extrapallial fluid in mussels with deformed and non-deformed shells; concentrations of algal cells were ranged from about 200 mL −1 in mussels with actually non-deformed shells to concentrations reaching up to 3.8 × 10 7 mL −1 in mussels with heavily deformed ones. Chemical analyses of soft tissues led us to conclude that butyltin compounds and trace metals cannot be considered among factors responsible for the shell deformity observed. Using scanning electron microscopy, the biogenic nature of the erosion on the external shell surface and aragonitic lenses of prisms in the curvature zone of deformed shells (in sections) were recorded. The sequence of the green algae from M. edulis of the Lower St. Lawrence Estuary was closely related to Coccomyxa sp. infecting M. edulis from the Flensburg Fjord (North Sea) and Modiolus modiolus (L.) from the Vityaz Bay (Sea of Japan).
Kristin Palmqvist - One of the best experts on this subject based on the ideXlab platform.
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Symbiosis constraints : Strong mycobiont control limits nutrient response in lichens
Ecology and evolution, 2017Co-Authors: Kristin Palmqvist, Oskar Franklin, Torgny NäsholmAbstract:Symbioses such as lichens are potentially threatened by drastic environmental changes. We used the lichen Peltigera aphthosaa symbiosis between a fungus (mycobiont), a green alga (Coccomyxa sp.), a ...
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Photosynthetic carbon acquisition in the lichen photobionts Coccomyxa and Trebouxia (Chlorophyta)
Physiologia Plantarum, 1997Co-Authors: Kristin Palmqvist, Asunción De Los Ríos, Carmen Ascaso, Göran SamuelssonAbstract:Processes involved in photosynthetic CO2 acquisition were characterised for the isolated lichen photobiont Trebouxia erici (Chlorophyta, Trebouxiophyceae) and compared with Coccomyxa (Chlorophyta), ...
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Characterisation of inorganic carbon fluxes, carbonic anhydrase(s) and ribulose-1,5-biphosphate carboxylase-oxygenase in the green unicellular alga Coccomyxa
Planta, 1995Co-Authors: Kristin Palmqvist, Dieter Sültemeyer, Pierre Andre Claude Baldet, T. John Andrews, Murray R BadgerAbstract:Processes involved in the uptake and fixation of dissolved inorganic carbon (DIC) were characterised for Coccomyxa, the green algal primary photobiont of the lichen Peltigera aphthosa and compared with the freeliving alga Chlamydomonas reinhardtii Dangeard (WT cc 125+). A mass-spectrometer disequilibrium technique was used to quantify fluxes of both HCO inf3 sup− and CO2 in the two algae, while activities of carbonic anhydrases (CAs) were examined in intact cells by measuring 18O exchange from doubly labelled CO2 (13C18O18O) to water and by using CA inhibitors. The CO2-fixation kinetics of intact Coccomyxa cells were also compared with the carboxylation efficiency of its isolated and purified primary carboxylating enzyme, ribulose-1,5-bisphosphate carboxylaseoxygenase (Rubisco). The two algae were found to be significantly different in their modes of acquiring CO2 for photosynthesis. Chlamydomonas was able to actively transport both HCO inf3 sup− and CO2 from the external medium, while Coccomyxa clearly favoured CO2 as a substrate. Both algae were found to possess external as well as internal CAs, but the relative amounts of these as well as their overall significance for the functioning of photosynthesis differed. In Coccomyxa, the internal CA activity was significantly higher than in Chlamydomonas and also predominated over the external activity. In Chlamydomonas, both transport and fixation of DIC were severely inhibited by ethoxyzolamide, an inhibitor of external and internal CAs as well as the DIC-transporting system, while this inhibitor only caused a minor inhibition of photosynthesis in Coccomyxa. These results thus give strong support for earlier indirect observations of the absence of a CO2concentrating mechanism in Coccomyxa. In addition, Coccomyxa was found to possess a Rubisco with a higher carboxylation efficiency than Chlamydomonas, having a Km (CO2) of 12 +3 μM CO2 and a CO2/O2 specificity factor (Sc/o) of 83 +2, and it may hence be concluded that the absence of the CO2-concentrating mechanism is positively correlated with a more efficient Rubisco in this alga.
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Purification and characterisation of an intracellular carbonic anhydrase from the unicellular green alga Coccomyxa
Planta, 1995Co-Authors: Thomas Hiltonen, Kristin Palmqvist, Jan Karlsson, Adrian K. Clarke, Göran SamuelssonAbstract:An intracellular carbonic anhydrase (CA; EC 4.2.1.1) was purified and characterised from the unicellular green alga Coccomyxa sp. Initial studies showed that cultured Coccomyxa cells contain an intracellular CA activity around 100 times higher than that measured in high-CO_2-grown cells of Chlamydomonas reinhardtii CW 92. Purification of a protein extract containing the CA activity was carried out using ammonium-sulphate precipitation followed by anion-exchange chromatography. Proteins were then separated by native (non-dissociating) polyacrylamide gel electrophoresis, with each individual protein band excised and assayed for CA activity. Measurements revealed CA activity associated with two discrete protein bands with similar molecular masses of 80 +5 kDa. Dissociation by denaturing polyacrylamide gel electrophoresis showed that both proteins contained a single polypeptide of 26 kDa, suggesting that each 80-kDa native protein was a homogeneous trimer. Isoelectric focusing of the 80-kDa proteins also produced a single protein band at a pH of 6.5. Inhibition studies on the purified CA extract showed that 50% inhibition of CA activity was obtained using 1 μM azetazolamide. Polyclonal antibodies against the 26-kDa CA were produced and shown to have a high specific binding to a single polypeptide in soluble protein extracts from Coccomyxa cells. The same antiserum, however, failed to cross-react with soluble proteins isolated from two different species of green algae, Chlamydomonas reinhardtii and Chlorella vulgaris. Correspondingly, antisera directed against pea chloroplastic CA, extracellular CA from C. reinhardtii and human CAII, showed no cross-hybridisation to the 26-kDa polypeptide in Coccomyxa. The 26-kDa protein was confirmed as being a CA by N-terminal sequencing of two internal polypeptide fragments and alignment of these sequences with that of previously identified CA proteins from several different species.
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Photobiont-related differences in carbon acquisition among green-algal lichens
Planta, 1994Co-Authors: Kristin Palmqvist, Göran Samuelsson, Murray R BadgerAbstract:The photosynthetic properties of a range of lichens (eight species) containing green algal primary photobionts of either the genus Coccomyxa, Dictyochloropsis or Trebouxia were examined with the ai ...