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François Thiaucourt - One of the best experts on this subject based on the ideXlab platform.

  • seroprevalence of Contagious Bovine Pleuropneumonia cbpp in mali
    Tropical Animal Health and Production, 2015
    Co-Authors: Amadou Sery, François Thiaucourt, Cheick Abou Kounta Sidibe, Ousmane Cisse, M B Diallo, Mamadou Kone, Agnes Waretszkuta, Francois Roger, Mamadou Niang
    Abstract:

    A serological survey to determine the prevalence of Contagious Bovine Pleuropneumonia (CBPP) in Mali was carried out by using the competitive enzyme linked-immunosorbent test (c-ELISA) on 8007 serum samples systematically collected from 199 cattle herds collected throughout the whole country. Results showed a national prevalence of 18.11 % at the individual level and 85.93 % at the herd level. Significant variations in the individual prevalence were observed between regions of the country and ranged from 4.63 % in Tombouctou to 54.88 % in Kidal. At the herd level, although there were variations between regions, a high prevalence was constantly observed ranging from 60 to 100 %, hence confirming the endemic nature of the disease across the country. The CBPP risk varied also between regions and was very low in Tombouctou (odds ratio (OR) = 0.4) but very high in Kidal (OR = 9.8). Similarly, the risk seemed higher in the animals of the over 3-year age group (OR = 1.6) compared to the other age groups. It was also observed that there was a slightly higher risk (OR = 1.3) in the females than in the males. This study confirms the presence of CBPP across the country and should help to elaborate strategies for the effective control of the disease.

  • characterization of anamnestic t cell responses induced by conventional vaccines against Contagious Bovine Pleuropneumonia
    PLOS ONE, 2013
    Co-Authors: Philippe Totte, Amadou Sery, Mamadou Niang, Aboubakar Yaya, Hezron Wesonga, Abel Wade, Jan Naessens, François Thiaucourt
    Abstract:

    A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against Contagious Bovine Pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4+ T lymphocytes. Moreover, CD62L expression indicated that both CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

  • experimental studies on the effect of long acting oxytetracycline treatment in the development of sequestra in Contagious Bovine Pleuropneumonia infected cattle
    Journal of Veterinary Medicine and Animal Health, 2010
    Co-Authors: Mamadou Niang, W Amanfu, Amadou Sery, Mamadou Kone, M Doucoure, Mapathe Ndiaye, François Thiaucourt
    Abstract:

    The effect of long-acting oxytetracycline treatment in the development of sequestra in Contagious Bovine Pleuropneumonia (CBPP)-infected animals was determined. Thirty five clinically healthy zebu cattle, negative for CBPP antibodies (as tested by a competitive ELISA), were infected with Mycoplasma mycoides subsp. mycoidessmall colony variant - field isolate. All the animals were monitored for clinical signs of illness and regularly sampled for serological and bacteriological analysis. Nine animals were treated with long-acting tetracycline upon the first evidence of clinical signs of illness. The 26 remaining animals were left untreated and served as control animals. Post-mortem examinations were performed to assess the presence or absence of CBPP gross lesions. The experimentation lasted for 10 months during which, 13 animals (all untreated) died with acute lung lesions. When the 22 surviving animals (13 untreated and 9 treated) were slaughtered at the end of experimentation, chronic lesions were observed in all of them. Among the 13 untreated animals, 10 had visible lung sequestra and 3 had cicatrical lesions indicative of resolved lung lesions. Conversely, among the 9 treated animals, only 1 had a small lung sequestrum and the remaining 8 had pulmonary adhesions. The results presented demonstrate that, under the experimental conditions used, treatment with oxytetracycline did not result in significant sequestra formation in CBPP-infected animals. Full field validation is required in order to confirm these findings.   Key words: Contagious Bovine Pleuropneumonia, antibiotherapy, oxytetracycline, lung sequestra, experimental transmission, Mali.

  • Seroprevalence of Contagious Bovine Pleuropneumonia in Ethiopian highlands (West Wellega zone, Bodji District)
    Ethiopian Veterinary Journal, 2005
    Co-Authors: Pascal Bonnet, Matthieu Lesnoff, François Thiaucourt, Asseguid Workalemahu, Daniel Kifle
    Abstract:

    Contagious Bovine Pleuropneumonia (CBPP) is a respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides small colony. CBPP has been and is still a major cause for concern for African countries (due to mortality, animal-production losses and cost of monitoring and control). A cross sectional study of the herd and animal seroprevalence of Contagious Bovine Pleuropneumonia (CBPP) using stratified sampling; competitive cELISA test was carried out in a zebu cattle-producing district of Ethiopia, representative of the Ethiopian highlands fringe where CBPP is assumed to re-emerge after the phasing out in 1994 of Rinderpest and CBPP bivalent vaccination program. A total of 2508 zebu cattle (Horro breed) from 300 herds were examined serologically and for characteristic clinical signs of CBPP. The herd seroprevalence was 4.6% in average representing 15 herds. The herd seroprevalence was ranging from 2.0% to 8.5% in 3 strata representing increasing herd size. In conclusion, the clinical signs observed in the survey were not providing a good picture on the disease prevalence. (Resume d'auteur)

  • Time-Delay Dynamics for Contagious Bovine Pleuropneumonia
    Acta Biotheoretica, 2004
    Co-Authors: Thomas Balenghien, Matthieu Lesnoff, François Thiaucourt, Karine Chalvet-monfray, Philippe Sabatier, Dominique Bicout
    Abstract:

    Modelling of Contagious disease usually employs compartmental SEIR-like models where the waiting times in respective compartments are exponentially distributed. In this paper, we are interested in investigating how the distributions of sojourn times in infective compartments affect the dynamics and persistence of the Contagious Bovine Pleuropneumonia, a chronic respiratory disease of cattle. Two kinds of extreme distributions of the sojourn times are considered: a Dirac delta-function and truncated Gaussian function leading to a model with (non-constant) delay and the classical exponential distribution that stands for a model without delay. Expressions of the basic reproductive numbers are derived and dynamical behaviours are discussed for the three models. It is found that the spreading of disease exhibits wave-like oscillations for the time-delay dynamics. In contrast, the disease appears to last longer when the spreading is described by the classical dynamics without delay. Subsequently, the time-delay dynamics turns out to be more appropriate for the description of an experimental epidemic of CBPP.

Robin A J Nicholas - One of the best experts on this subject based on the ideXlab platform.

  • a history of the prevalence and control of Contagious Bovine Pleuropneumonia in china
    Veterinary Journal, 2012
    Co-Authors: Yuan Li, Robin A J Nicholas, Chao Chen, Meijing Zhang, Hui Dong
    Abstract:

    Abstract Contagious Bovine Pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides small colony type, was once the most damaging infectious animal disease in China, second only to rinderpest. Between 1949 and 1989, 178,570 cattle died of CBPP, causing estimated losses of 356 million RMB (1 RMB = approx. £0.094, US$0.15, €0.11 at 27th January 2011). In 1956, in an effort to control the disease, a virulent strain of the causative organism (Ben-1) was attenuated by multiple passages in rabbits. The resultant vaccine achieved a high protection rate in cattle with a duration of immunity of 28 months. Vaccines were also prepared in sheep to increase the antigen yield and then in Tibetan sheep because it caused fewer adverse reactions in yaks and related species. The last CBPP infected animal was identified in 1989 since when no more cases have occurred. In 1992, vaccination of cattle was stopped. In 2008 China submitted an application to the World Organization for Animal Health (OIE) to be declared CBPP-free.

  • A simplified PCR method for genotyping Mycoplasma mycoides subspecies mycoides small colony: the aetiologic agent of Contagious Bovine Pleuropneumonia.
    Veterinary Microbiology, 2012
    Co-Authors: Colin P Churchward, Robin A J Nicholas, Roger D Ayling, Miroslav Hlúšek, Laura Mcauliffe
    Abstract:

    Mycoplasma mycoides subspecies mycoides small colony is the aetiological agent of Contagious Bovine Pleuropneumonia, a cattle disease endemic to areas of sub-Saharan Africa. Twenty isolates from various geographical locations and the type strain were analysed by multi-locus sequence analysis (MLSA). The data generated was then used to develop three PCR primer sets to differentiate these isolates. The PCRs differentiated the isolates into four groups; the type strain (T); isolates of European origin (Eu); isolates from Tanzania (Af1) with a final group consisting of isolates from Namibia and Botswana (Af2). These PCRs offers a rapid and efficient post-identification typing method without the need to sequence and analyse multiple genes.

  • Contagious Bovine Pleuropneumonia--a reminder.
    The Veterinary record, 2009
    Co-Authors: Robin A J Nicholas, Roger D Ayling, Laura Mcauliffe
    Abstract:

    Many readers may consider Contagious Bovine Pleuropneumonia (CBPP) or lung sickness to be an exotic or historical disease, which has long been eradicated from Britain and Europe. Older readers may have seen CBPP during secondments to Africa in the 1980s, when the disease was largely under control.

  • impact of mycoplasma bovis infection on serosurveillance for Contagious Bovine Pleuropneumonia
    Veterinary Record, 2008
    Co-Authors: Rosario Goncalves, Jose Regalla, Roger D Ayling, Robin A J Nicholas
    Abstract:

    Contagious Bovine Pleuropneumonia (cbpp), caused by Mycoplasma mycoides subspecies mycoides , affected several European countries in the late 20th century, including Portugal, where the last European outbreak occurred in 1999. The limited sensitivity and specificity of cbpp serological testing has

  • assessing the in vitro effectiveness of antimicrobials against mycoplasma mycoides subsp mycoides small colony type to reduce Contagious Bovine Pleuropneumonia infection
    Antimicrobial Agents and Chemotherapy, 2005
    Co-Authors: Roger D Ayling, John B March, S Bisgaardfrantzen, Kevin Godinho, Robin A J Nicholas
    Abstract:

    In vitro minimum inhibitory concentrations were determined for 21 antimicrobials against 41 isolates of Mycoplasma mycoides subsp. mycoides small-colony type, the cause of Contagious Bovine Pleuropneumonia. Of the antimicrobials used most widely in Africa, oxytetracycline and tilmicosin were effective, while the isolates were resistant to tylosin. These results provide a baseline for monitoring antimicrobial resistance.

Massimo Scacchia - One of the best experts on this subject based on the ideXlab platform.

  • Contagious Bovine Pleuropneumonia: A Comprehensive Overview.
    Veterinary Pathology, 2020
    Co-Authors: Giovanni Di Teodoro, Flavio Sacchini, Andrea Di Provvido, Giuseppe Marruchella, Anna Rita D'angelo, Gianluca Orsini, Paola Di Giuseppe, Massimo Scacchia
    Abstract:

    Contagious Bovine Pleuropneumonia (CBPP) is a respiratory disease of cattle that is listed as notifiable by the World Organization for Animal Health. It is endemic in sub-Saharan Africa and causes important productivity losses due to the high mortality and morbidity rates. CBPP is caused by Mycoplasma mycoides subsp. mycoides (Mmm) and is characterized by severe fibrinous bronchopneumonia and pleural effusion during the acute to subacute stages and by pulmonary sequestra in chronic cases. Additional lesions can be detected in the kidneys and in the carpal and tarsal joints of calves. Mmm infection occurs through the inhalation of infected aerosol droplets. After the colonization of bronchioles and alveoli, Mmm invades blood and lymphatic vessels and causes vasculitis. Moreover, Mmm can be occasionally demonstrated in blood and in a variety of other tissues. In the lung, Mmm antigen is commonly detected on bronchiolar and alveolar epithelial cells, in lung phagocytic cells, within the wall of blood and lymphatic vessels, inside necrotic areas, and within tertiary lymphoid follicles. Mmm antigen can also be present in the cytoplasm of macrophages within lymph node sinuses, in the germinal center of lymphoid follicles, in glomerular endothelial cells, and in renal tubules. A complete pathological examination is of great value for a rapid presumptive diagnosis, but laboratory investigations are mandatory for definitive diagnosis. The purpose of this review is to describe the main features of CBPP including the causative agent, history, geographic distribution, epidemiology, clinical course, diagnosis, and control. A special focus is placed on gross and microscopic lesions in order to familiarize veterinarians with the pathology and pathogenesis of CBPP.

  • Lung lesion score system in cattle: proposal for Contagious Bovine Pleuropneumonia
    Tropical Animal Health and Production, 2018
    Co-Authors: Andrea Di Provvido, Giovanni Di Teodoro, Geoffrey Muuka, Giuseppe Marruchella, Massimo Scacchia
    Abstract:

    Contagious Bovine Pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides . The peculiar pathological features of CBPP make desirable the assessment of ad hoc score methods to grade the disease in the affected animals. Thus, the present work aims to assess a new lung score system for CBPP. Our results indicate that the present score system strongly correlates with that previously published by Turner and could be effectively used in CBPP-affected animals.

  • Polymorphonuclear cells and reactive oxygen species in Contagious Bovine Pleuropneumonia: New insight from in vitro investigations.
    Veterinary Immunology and Immunopathology, 2018
    Co-Authors: Giovanni Di Teodoro, Flavio Sacchini, Andrea Di Provvido, Giuseppe Marruchella, Francesco Mosca, Pietro Giorgio Tiscar, Massimo Scacchia
    Abstract:

    Abstract Reactive oxygen species (ROS) are suggested to play a role in the pathogenesis of Contagious Bovine Pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides ( Mmm ). The present study investigated the generation of ROS by different strains of Mmm , as well as their effect on the oxidative response of Bovine neutrophils. The production of ROS was indirectly measured using a luminol-based chemiluminescence assay. Our results confirm that Mmm can produce ROS via the metabolism of glycerol, significant differences existing between African and European strains. Mmm was capable of adhering to the external surface of neutrophils. Interestingly, Mmm enhanced the respiratory burst of Bovine neutrophils. This activity was particularly pronounced with the African field strain and in presence of glycerol. Taken together, our data argue in favour of a major role for neutrophils as the main source of ROS in Contagious Bovine Pleuropneumonia.

  • whole genome sequencing of mycoplasma mycoides subsp mycoides italian strain 57 13 the causative agent of Contagious Bovine Pleuropneumonia
    Genome Announcements, 2015
    Co-Authors: Massimiliano Orsini, Ivanka Krasteva, Massimo Scacchia, Andrea Ciammaruconi, Bernardina Gentile, Florigio Lista, Massimo Ancora, Maurilia Marcacci, Alessandro Pini, Flavio Sacchini
    Abstract:

    ABSTRACT Mycoplasma mycoides subsp. mycoides is generally considered one of most pathogenic Mycoplasma species, and it is the etiological agent of Contagious Bovine Pleuropneumonia (CBPP). Here, we present the annotated genome sequence of M. mycoides subsp. mycoides Italian strain 57/13, isolated in 1992 during CBPP outbreaks in Italy.

  • Whole-Genome Sequencing of Mycoplasma mycoides subsp. mycoides Italian Strain 57/13, the Causative Agent of Contagious Bovine Pleuropneumonia.
    Genome Announcements, 2015
    Co-Authors: Massimiliano Orsini, Ivanka Krasteva, Massimo Scacchia, Andrea Ciammaruconi, Bernardina Gentile, Florigio Lista, Massimo Ancora, Maurilia Marcacci, Alessandro Pini, Flavio Sacchini
    Abstract:

    ABSTRACT Mycoplasma mycoides subsp. mycoides is generally considered one of most pathogenic Mycoplasma species, and it is the etiological agent of Contagious Bovine Pleuropneumonia (CBPP). Here, we present the annotated genome sequence of M. mycoides subsp. mycoides Italian strain 57/13, isolated in 1992 during CBPP outbreaks in Italy.

Flavio Sacchini - One of the best experts on this subject based on the ideXlab platform.

  • Contagious Bovine Pleuropneumonia: A Comprehensive Overview.
    Veterinary Pathology, 2020
    Co-Authors: Giovanni Di Teodoro, Flavio Sacchini, Andrea Di Provvido, Giuseppe Marruchella, Anna Rita D'angelo, Gianluca Orsini, Paola Di Giuseppe, Massimo Scacchia
    Abstract:

    Contagious Bovine Pleuropneumonia (CBPP) is a respiratory disease of cattle that is listed as notifiable by the World Organization for Animal Health. It is endemic in sub-Saharan Africa and causes important productivity losses due to the high mortality and morbidity rates. CBPP is caused by Mycoplasma mycoides subsp. mycoides (Mmm) and is characterized by severe fibrinous bronchopneumonia and pleural effusion during the acute to subacute stages and by pulmonary sequestra in chronic cases. Additional lesions can be detected in the kidneys and in the carpal and tarsal joints of calves. Mmm infection occurs through the inhalation of infected aerosol droplets. After the colonization of bronchioles and alveoli, Mmm invades blood and lymphatic vessels and causes vasculitis. Moreover, Mmm can be occasionally demonstrated in blood and in a variety of other tissues. In the lung, Mmm antigen is commonly detected on bronchiolar and alveolar epithelial cells, in lung phagocytic cells, within the wall of blood and lymphatic vessels, inside necrotic areas, and within tertiary lymphoid follicles. Mmm antigen can also be present in the cytoplasm of macrophages within lymph node sinuses, in the germinal center of lymphoid follicles, in glomerular endothelial cells, and in renal tubules. A complete pathological examination is of great value for a rapid presumptive diagnosis, but laboratory investigations are mandatory for definitive diagnosis. The purpose of this review is to describe the main features of CBPP including the causative agent, history, geographic distribution, epidemiology, clinical course, diagnosis, and control. A special focus is placed on gross and microscopic lesions in order to familiarize veterinarians with the pathology and pathogenesis of CBPP.

  • Polymorphonuclear cells and reactive oxygen species in Contagious Bovine Pleuropneumonia: New insight from in vitro investigations.
    Veterinary Immunology and Immunopathology, 2018
    Co-Authors: Giovanni Di Teodoro, Flavio Sacchini, Andrea Di Provvido, Giuseppe Marruchella, Francesco Mosca, Pietro Giorgio Tiscar, Massimo Scacchia
    Abstract:

    Abstract Reactive oxygen species (ROS) are suggested to play a role in the pathogenesis of Contagious Bovine Pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides ( Mmm ). The present study investigated the generation of ROS by different strains of Mmm , as well as their effect on the oxidative response of Bovine neutrophils. The production of ROS was indirectly measured using a luminol-based chemiluminescence assay. Our results confirm that Mmm can produce ROS via the metabolism of glycerol, significant differences existing between African and European strains. Mmm was capable of adhering to the external surface of neutrophils. Interestingly, Mmm enhanced the respiratory burst of Bovine neutrophils. This activity was particularly pronounced with the African field strain and in presence of glycerol. Taken together, our data argue in favour of a major role for neutrophils as the main source of ROS in Contagious Bovine Pleuropneumonia.

  • whole genome sequencing of mycoplasma mycoides subsp mycoides italian strain 57 13 the causative agent of Contagious Bovine Pleuropneumonia
    Genome Announcements, 2015
    Co-Authors: Massimiliano Orsini, Ivanka Krasteva, Massimo Scacchia, Andrea Ciammaruconi, Bernardina Gentile, Florigio Lista, Massimo Ancora, Maurilia Marcacci, Alessandro Pini, Flavio Sacchini
    Abstract:

    ABSTRACT Mycoplasma mycoides subsp. mycoides is generally considered one of most pathogenic Mycoplasma species, and it is the etiological agent of Contagious Bovine Pleuropneumonia (CBPP). Here, we present the annotated genome sequence of M. mycoides subsp. mycoides Italian strain 57/13, isolated in 1992 during CBPP outbreaks in Italy.

  • Whole-Genome Sequencing of Mycoplasma mycoides subsp. mycoides Italian Strain 57/13, the Causative Agent of Contagious Bovine Pleuropneumonia.
    Genome Announcements, 2015
    Co-Authors: Massimiliano Orsini, Ivanka Krasteva, Massimo Scacchia, Andrea Ciammaruconi, Bernardina Gentile, Florigio Lista, Massimo Ancora, Maurilia Marcacci, Alessandro Pini, Flavio Sacchini
    Abstract:

    ABSTRACT Mycoplasma mycoides subsp. mycoides is generally considered one of most pathogenic Mycoplasma species, and it is the etiological agent of Contagious Bovine Pleuropneumonia (CBPP). Here, we present the annotated genome sequence of M. mycoides subsp. mycoides Italian strain 57/13, isolated in 1992 during CBPP outbreaks in Italy.

Roger D Ayling - One of the best experts on this subject based on the ideXlab platform.