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Ramesh Chander Kuhad - One of the best experts on this subject based on the ideXlab platform.
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biochemical characterization and molecular evidence of a laccase from the bird s nest fungus Cyathus bulleri
Fungal Genetics and Biology, 2005Co-Authors: Kavita Vasdev, Shikha Dhawan, Rajeev Kumar Kapoor, Ramesh Chander KuhadAbstract:Abstract Cyathus bulleri, a bird’s nest fungus, known to decolorize polymeric dye Poly R-478, was found to produce 8 U ml−1 of laccase in malt extract broth. Laccase activity appeared as a single band on non-denaturing gel. Laccase was purified to homogeneity by anion exchange chromatography and gel filtration. The enzyme was a monomer with an apparent molecular mass of 60 kD, pI of 3.7 and was stable in the pH range of 2–6 with an optimum pH of 5.2. The optimal reaction temperature was 45 °C and the enzyme lost its activity above 70 °C. Enzyme could oxidize a broad range of various phenolic substrates. Km values for ABTS, 2,6-dimethoxyphenol, guaiacol, and ferulic acid were found to be 48.6, 56, 22, and 14 mM while Kcat values were 204, 180, 95.6, and 5.2, respectively. It was completely inhibited by KCN, NaN3, β-mercaptoethanol, HgCl2, and SDS, while EDTA had no effect on enzyme activity. The N-terminal amino acid sequence of C. bulleri laccase showed close homology to N-terminal sequences of laccase from other white-rot fungi. A 150 bp gene sequence encoding copper-binding domains I and II was most similar to the sequence encoding a laccase from Pycnoporus cinnabarinus with 74.8% level of similarity.
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Effect of antibiotics on growth and laccase production from Cyathus bulleri and Pycnoporus cinnabarinus
Bioresource technology, 2005Co-Authors: Shikha Dhawan, Rup Lal, Manjit Hanspal, Ramesh Chander KuhadAbstract:The effect of nine different antibiotics (chloramphenicol, ampicillin trihydrate, kanamycin A monosulfate, neomycin sulfate, erythromycin, thiostrepton, tetracycline, apramycin sulfate and streptomycin sulfate) on growth and laccase production from Cyathus bulleri and Pycnoporus cinnabarinus has been investigated. All the antibiotics tested at a concentration of 200 mg/l affected the fungal growth, release of protein and laccase production to different extent. Inhibition in fungal growth was found to be positively correlated with increase in laccase production. Interestingly, apramycin sulfate inhibited biomass production (14.9-26.2%), nevertheless, it stimulated maximum laccase production (18.2 U/ml) in both the fungi. Increasing concentrations of apramycin sulfate enhanced laccase production from P. cinnabarinus but not from C. bulleri.
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ethidium bromide stimulated hyper laccase production from bird s nest fungus Cyathus bulleri
Letters in Applied Microbiology, 2003Co-Authors: Shikha Dhawan, Rup Lal, Ramesh Chander KuhadAbstract:S . D H A W A N , R . L A L A N D R . C . K U H A D . 2003. Aims: Effect of ethidium bromide, a DNA intercalating agent, on laccase production from Cyathus bulleri was studied. Methods and Results: The bird’s nest fungus, Cyathus bulleri was grown on 2% (w ⁄ v) malt extract agar (MEA) supplemented with 1AE5 l gm l )1 of the phenanthridine dye ethidium bromide (EtBr) for 7 d and when grown subsequently in malt extract broth (MEB), produced a 4AE2-fold increase in laccase production as compared to the untreated fungus. The fungal cultures following a single EtBr treatment, when regrown on MEA devoid of EtBr, produced a sixfold increase in laccase in MEB. However, on subsequent culturing on MEA in the absence of EtBr, only a 2AE5-fold increase in laccase production could be maintained. In another attempt, the initial EtBr-treated cultures, when subjected to a second EtBr treatment (1AE5 l gm l )1 ) on MEA for 7 d, produced a 1AE4-fold increase in laccase production in MEB. Conclusions: The white-rot fungus Cyathus bulleri, when treated with EtBr at a concentration of 1AE5 l gm l )1 and regrown on MEA devoid of EtBr, produced a sixfold increase in laccase production in MEB. Significance and Impact of the Study: The variable form of C. bulleri capable of hyper laccase production can improve the economic feasibility of environmentally benign processes involving use of fungal laccases in cosmetics (including hair dyes), food and beverages, clinical diagnostics, pulp and paper industry, industrial effluent treatment, animal biotechnology and biotransformations.
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effect of amino acids and vitamins on laccase production by the bird s nest fungus Cyathus bulleri
Bioresource Technology, 2002Co-Authors: Shikha Dhawan, Ramesh Chander KuhadAbstract:Various amino acids, their analogues and vitamins have shown stimulatory as well as inhibitory effects on laccase production by Cyathus bulleri. DL-methionine, DL-tryptophan, glycine and DL-valine stimulated laccase production, while L-cysteine monohydrochloride completely inhibited the enzyme production. Among vitamins tested biotin, riboflavin and pyridoxine hydrochloride were found to induce laccase production.
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decolorization of triphenylmethane dyes by the bird s nest fungus Cyathus bulleri
Current Microbiology, 1995Co-Authors: Kavita Vasdev, Ramesh Chander Kuhad, R K SaxenaAbstract:Decolorization of three triphenylmethane dyes by three bird's nest fungi—Cyathus bulleri, C. stercoreus, and C. striatus—was studied. Cyathus bulleri was found to be the most efficient in decolorization as demonstrated by the disappearance of the dyes from cultures, monitored by decreases in absorbance. Growth of the Cyathus spp. was not affected by the presence of dyes in the cultures. Decolorization of dyes was also observed with extracellular culture filtrates, indicating exocellular activity. Laccase activity, tested in replicate cultures, was found to be maximum during the decolorization period. Nitrogen in the medium had no effect on decolorization.
Jinming Gao - One of the best experts on this subject based on the ideXlab platform.
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terpenoids with neurotrophic and anti neuroinflammatory activities from the cultures of the fungus Cyathus stercoreus
Natural Product Research, 2020Co-Authors: Xia Yin, Rongwei Kou, Weiwei Wang, Zean Bao, Jinming GaoAbstract:Three new cyathane diterpenoids named stercorins A–C (1–3), including one with unusual 4,9-seco-carbon skeleton, together with two new drimane sesquiterpenoids named stercorins D (4) and E (5) were...
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cyathane diterpenes from cultures of the bird s nest fungus Cyathus hookeri and their neurotrophic and anti neuroinflammatory activities
Journal of Natural Products, 2019Co-Authors: Dan Tang, Marc Stadler, Weiwei Wang, Bo Liu, Zhi Yang, Lingli Liu, Jinming GaoAbstract:Six new cyathane diterpenoids, cyahookerins A–F (1–6), as well as nine known analogues (7–15), were isolated from the liquid culture of the basidiomycete Cyathus hookeri. Their structures were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, HRESIMS, and ECD), and the absolute configurations of compounds 1 and 4 were determined by single-crystal X-ray crystallography. Compounds 1 and 2 represent the first unusual cyathane acetals featuring a dioxolane ring. Compounds 1–6 displayed differential nerve growth factor-induced neurite outgrowth-promoting activity in PC-12 cells at concentrations of 10 μM. In addition, cyahookerin B (2), cyathin E (9), cyathin B2 (12), and cyathin Q (13) showed significant nitric oxide production inhibition in Lipopolysaccharide (LPS)-activated BV-2 microglial cells with IC50 values of 12.0, 6.9, 10.9, and 9.1 μM, respectively. Similar binding modes of the four compounds were indicated by molecular-docking studies, and structure–activity relationships ...
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new cyathane diterpenoids with neurotrophic and anti neuroinflammatory activity from the bird s nest fungus Cyathus africanus
Fitoterapia, 2019Co-Authors: Xia Yin, Jing Wei, Marc Stadler, Rongwei Kou, Weiwei Wang, Yuqi Gao, Jinming GaoAbstract:Abstract Eleven new cyathane diterpenoids, designated cyafricanins A–K (1−11), were isolated from the culture broth of the baisidiomycete Cyathus africanus (Nidulariaceae, Bird's nest fungi). Their structures were elucidated by comprehensive analysis of their NMR and HRESIMS data. Cyafricanins A (1) was found to possess an unusual 3,4-seco‑carbon skeleton. All compounds were evaluated for their neurotrophic activity in PC-12 cells and anti-neuroinflammatory activity in BV2 microglia cells. All of the diterpenoids showed nerve growth factor induced neurite outgrowth-promoting activity at concentration of 20 μM. Among them, cyafricanin B (2) and cyafricanin G (7) exhibited promising neurotrophic activity, and cyafricanin A (1) showed strong inhibitory effects on both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, molecular docking studies revealed that cyafricanin A (1) showed strong interactions with the iNOs protein in the active cavity.
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cyathane diterpenoids and drimane sesquiterpenoids with neurotrophic activity from cultures of the fungus Cyathus africanus
The Journal of Antibiotics, 2019Co-Authors: Rongwei Kou, Xia Yin, Xitao Yan, Qiang Zhang, Chenyu Cao, Jinming GaoAbstract:Five terpenoids, including two new cyathane diterpenoids neocyathin S (1) and neocyathin T (2), together with three drimane sesquiterpenoids, one known 3β,6β-dihydroxycinnamolide (3), two new ones 3β,6α-dihydroxycinnamolide (4) and 2-keto-3β,6β-dihydroxycinnamolide (5), were isolated from the cultures of the basidiomycete Cyathus africanus. Their structures were established based on extensive spectroscopic methods including 2D NMR (HSQC, 1H‒1H-COSY, HMBC, ROESY) and HRESIMS experiments. The absolute configurations of two pairs of epimers, 1 and 2 as well as 3 and 4, were determined by ECD quantum chemical calculation. All the five compounds enhanced nerve growth factor (NGF)-mediated neurite outgrowth using rat pheochromocytoma (PC12) cells at concentration 10 µM.
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molecular diversity and potential anti neuroinflammatory activities of cyathane diterpenoids from the basidiomycete Cyathus africanus
Scientific Reports, 2017Co-Authors: Jing Wei, Qiang Zhang, Yuanyuan Cheng, Wanhui Guo, Dacheng Wang, Jianhui Rong, Jinming GaoAbstract:Ten new polyoxygenated cyathane diterpenoids, named neocyathins A–J (1–10), together with four known diterpenes (11–14), were isolated from the liquid culture of the medicinal basidiomycete fungus Cyathus africanus. The structures and configurations of these new compounds were elucidated through comprehensive spectroscopic analyses including 1D NMR, 2D NMR (HSQC, HMBC, NOESY) and HRESIMS, and electronic circular dichroism (ECD) data. Neuroinflammation is implicated in the pathogenesis of various neurodegenerative diseases, such as Alzheimers’ disease (AD). All isolated compounds were evaluated for the potential anti-neuroinflammatory activities in BV2 microglia cells. Several compounds showed differential effects on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-stimulated and Aβ1–42-treated mouse microglia cell line BV-2. Molecular docking revealed that bioactive compounds (e.g., 11) could interact with iNOS protein other than COX-2 protein. Collectively, our results suggested that this class of cyathane diterpenoids might serve as important lead compounds for drug discovery against neuroinflammation in AD.
Karl-erik Eriksson - One of the best experts on this subject based on the ideXlab platform.
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production of ligninolytic enzymes and synthetic lignin mineralization by the bird s nest fungus Cyathus stercoreus
Applied Microbiology and Biotechnology, 1999Co-Authors: Anand Sethuraman, Danny E. Akin, Karl-erik ErikssonAbstract:Production of ligninolytic enzymes and degradation of 14C-ring labeled synthetic lignin by the white-rot fungus Cyathus stercoreus ATCC 36910 were determined under a variety of conditions. The highest mineralization rate for 14C dehydrogenative polymerizates (DHP; 38% 14CO2 after 30 days) occurred with 1 mM ammonium tartrate as nitrogen source and 1% glucose as additional carbon source, but levels of extracellular laccase and manganese peroxidase (MnP) were low. In contrast, 10 mM ammonium tartrate with 1% glucose gave low mineralization rates (10% 14CO2 after 30 days) but higher levels of laccase and manganese peroxidase. Lignin peroxidase was not produced by C. stercoreus under any of the studied conditions. Mn(II) at 11 ppm gave a higher rate of 14C DHP mineralization than 0.3 or 40 ppm, but the highest manganese peroxidase level was obtained with Mn(II) at 40 ppm. Cultivation in aerated static flasks gave rise to higher levels of both laccase and manganese peroxidase compared to the levels in shake cultures. 3,4-Dimethoxycinnamic acid at 500 μM concentration was the most effective inducer of laccase of those tested. The purified laccase was a monomeric glycoprotein having an apparent molecular mass of 70 kDa, as determined by calibrated gel filtration chromatography. The pH optimum and isoelectric point of the purified laccase were 4.8 and 3.5, respectively. The N-terminal amino acid sequence of C. stercoreus laccase showed close homology to the N-terminal sequences determined from other basidiomycete laccases. Information on C. stercoreus, whose habitat and physiological requirements for lignin degradation differ from many other white-rot fungi, expands the possibilities for industrial application of biological systems for lignin degradation and removal in biopulping and biobleaching processes.
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Effect of aromatic compounds on growth and ligninolytic enzyme production of two white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus
Canadian Journal of Microbiology, 1998Co-Authors: Anand Sethuraman, Danny E. Akin, Jason G Eisele, Karl-erik ErikssonAbstract:Seven benzoic acid, ten cinnamic acid, and five benzaldehyde derivatives were tested for their effects on hyphal growth and production of laccase and manganese peroxidase by Ceriporiopsis subvermispora FP 90031-sp and Cyathus stercoreus ATCC 36910. Derivatives tested included phenolic compounds and their corresponding unsubstituted and O-methylated derivatives. Benzaldehyde derivatives were more toxic to both fungi than the corresponding benzoic and cinnamic acid derivatives. Hyphal growth was generally increased at a low concentration of 1 mM, while higher concentrations of 5-10 mM mostly resulted in less or no growth. Hyphal growth and enzyme production response were compound specific. However, generally monomethoxylated compounds were more toxic than compounds with an additional methoxyl group. Cyathus stercoreus was more sensitive than Ceriporiopsis subvermispora to most of the compounds tested and thus showed poorer growth. Cyathus stercoreus produced higher concentrations of manganese peroxidase tha...
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Biological delignification of plant components by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus
Animal Feed Science and Technology, 1996Co-Authors: Danny E. Akin, Anand Sethuraman, W.h. Morrison, Luanne L. Rigsby, Gary R. Gamble, Karl-erik ErikssonAbstract:Lignocelluloses from diverse plant types were treated with the white rot fungi Ceriporiopsis subvermispora (strains CZ-3-8497 and FP-90031-sp) and Cyathus stercoreus. Sources of lignocellulose included: the warm-season grasses sorghum (leaf blades, sheaths, and stems), pearl millet, napiergrass, and maize (stems); the cool-season grass wheat (leaf blades, sheaths, and stems); the legumes alfalfa (stems) and lespedeza (leaflets and stems). Fungus-treated residues were compared with untreated, control samples and with plants treated with a non-delignifying isolate of Trichoderma. Residues were evaluated for improved biodegradability by ruminal microorganisms and modifications in cell wall chemistry by nuclear magnetic resonance, gas chromatography, and ultraviolet absorption microspectrophotometry. Specific plant—fungus interactions were identified that resulted in selective removal of lignin and improved biodegradability by white rot fungi but not the Trichoderma sp. All white rot fungi removed ester-linked p-coumaric and ferulic acids from grass stems, and this phenomenon appeared to account for the significant reduction in aromatic components and improved biodegradability of fungus-treated grass lignocellulose. Cell walls in alfalfa stems were more resistant to biological delignification than those in grasses, with only C. stercoreus removing significant amounts of aromatics and improving biodegradability. All white rot fungi improved the biodegradability of tannin-rich lespedeza samples.
Saroj Mishra - One of the best experts on this subject based on the ideXlab platform.
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An insight into transcriptome of Cyathus bulleri for lignocellulase expression on wheat bran
Archives of Microbiology, 2021Co-Authors: Arpita Vats, Saroj MishraAbstract:To identify enzymes that can be effectively used for hydrolysis of lignocellulosic biomass, an attractive carbon source in biorefineries, transcriptome analysis was carried out of wheat bran grown fungus, Cyathus bulleri . A comprehensive set of transcripts, encoding carbohydrate active enzymes, were identified. These belonged to 55, 32, 12, 11 and 7 different families of the enzyme classes of Glycoside Hydrolases (GHs), Glycosyl Transferases (GTs), Auxiliary Activities (AAs), Carbohydrate Esterases (CEs) and Polysaccharide Lyases (PLs) respectively . Higher levels of transcripts were obtained for proteins encoding cellulose and hemicellulose degrading activities (of the GH class) with the highest diversity found in the transcripts encoding the hemicellulases. Several transcripts encoding pectin degrading activity were also identified indicating close association of the pectin with the cellulose/hemicellulose in the cell wall of this fungus. Transcripts encoding ligninases were categorized into Cu radical oxidase, Glucose-Methanol-Choline oxidoreductase (with 37 different transcripts in the AA3 sub-family), Laccase and Manganese peroxidases. Temporal gene expression profile for laccase isoforms was studied to understand their role in lignin degradation. To our knowledge, this is the first analysis of the transcriptome of a member belonging to the family Nidulariaceae.
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laccase isoform diversity on basal medium in Cyathus bulleri and role in decolorization detoxification of textile dyes and effluent
World Journal of Microbiology & Biotechnology, 2020Co-Authors: A Vats, Saroj MishraAbstract:Laccases (EC 1.10.3.2) are multi-copper oxidases that can degrade several xenobiotics, including textile dyes. Present study investigated the nature of laccase isoforms induced by 2,6-dimethylaniline in Cyathus bulleri cultivated on basal salt medium. Two isoforms, LacI and LacII were identified and purified by a combination of ultrafiltration and ion-exchange chromatography. The MS spectrum of the two proteins displayed a number of non-identical and identical molecular peaks (m/z), and, the latter were mapped to protein originating from the previously reported Laccase (Lcc) 1 gene. The LacI isoform exhibited higher catalytic efficiency (Kcat/Km) towards 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, guaiacol and pyrogallol and was tolerant to high levels of chloride ions and resistant to EDTA. Higher decolorization of several dyes such as Direct Scarlet B (67%), Reactive Brilliant blue-R (96%), Direct Orange 34 (50%) and Reactive Red198 (95%) by the LacI isoform makes it a good candidate for degradation of synthetic dyes. The decolorization of Direct Orange 34 by laccases is being reported for the first time. Many of the properties exhibited by this isoform make it a good candidate for large scale production and applications for use in the dyeing industry.
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investigation of the role of central metal ion of Cyathus bulleri laccase 1 using guanidinium chloride induced denaturation
International Journal of Biological Macromolecules, 2019Co-Authors: Sumbul Afreen, Danish Idrees, Radhika Khera, Mohd Amir, Imtaiyaz Hassan, Saroj MishraAbstract:The structure and folding/unfolding kinetics of Cyathus bulleri laccase 1 (Lcc1), expressed in Pichia pastoris, were analyzed by spectroscopic methods to understand the role of central metal ion. Far UV CD structure analysis revealed major β-sheet and minor α helical segments present in the Lcc1. A significant change in the spectrum of Lcc1, indicative of unfolding of secondary structures, was observed with increasing concentrations of guanidinium chloride (GdnHCl) during Trp fluorescence, absorption and CD measurements. A similar trend was also noticed for enzyme activity with respect to GdnHCl concentrations. To establish the role of copper ion in the catalytic activity of laccase, a complete removal of copper was carried out and addition of copper was found to restore the structure and activity during the refolding process. The apo form was also reconstituted by addition of zinc ion which restored nearly 84% of enzyme activity, indicating non-essential role of copper in maintaining conformation and activity. Structural studies and inductively coupled plasma mass spectrometry data supported these observations.
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identification and evaluation of bioremediation potential of laccase isoforms produced by Cyathus bulleri on wheat bran
Journal of Hazardous Materials, 2018Co-Authors: Arpita Vats, Saroj MishraAbstract:Abstract Multiplicity in laccases among lignin degrading fungal species is of interest as it confers the ability to degrade several types of lignocellulosics. The combination of laccases produced on such substrates could be beneficial for treatment of complex aromatics, including dyes. In this study, we report on production of high units (679.6 U g−1 substrate) of laccase on solid wheat bran (WB) by Cyathus bulleri. Laccase, purified from the culture filtrates of WB grown fungus, was effective for oxidation of veratryl alcohol, Reactive blue 21 and textile effluent without assistance of externally added mediators. De novo sequencing of the ‘purified’ laccase lead to identification of several peptides that originated from different laccase genes. Transcriptome analysis of the fungus, cultivated on WB, confirmed presence of 8 isozymes, that were re-amplified and sequenced from the cDNA prepared from WB grown fungus. The 8 isozymes were grouped into 3 classes, based on their sequence relationship with other basidiomycete laccases. The isoforms produced on WB decolorized (by ∼57%) and degraded textile effluent far more effectively, compared to laccase obtained from Basal salt cultivated fungus. The decolorization and degradation was also accompanied by more than 95% reduction in phytotoxicity.
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decolorization of complex dyes and textile effluent by extracellular enzymes of Cyathus bulleri cultivated on agro residues domestic wastes and proposed pathway of degradation of kiton blue a and reactive orange 16
Environmental Science and Pollution Research, 2017Co-Authors: Arpita Vats, Saroj MishraAbstract:In this study, the white-rot fungus Cyathus bulleri was cultivated on low-cost agro-residues, namely wheat bran (WB), wheat straw (WS), and domestic waste orange peel (OP) for production of ligninolytic enzymes. Of the three substrates, WB and OP served as good materials for the production of laccase with no requirement of additional carbon or nitrogen source. Specific laccase activity of 94.4 U mg−1 extracellular protein and 21.01 U mg−1 protein was obtained on WB and OP, respectively. Maximum decolorization rate of 13.6 μmol h−1 U−1 laccase for reactive black 5 and 22.68 μmol h−1 U−1 laccase for reactive orange 16 (RO) was obtained with the WB culture filtrate, and 11.7 μmol h−1 U−1 laccase for reactive violet 5 was observed with OP culture filtrate. Importantly, Kiton blue A (KB), reported not to be amenable to enzymatic degradation, was degraded by culture filtrate borne activities. Products of degradation of KB and RO were identified by mass spectrometry, and a pathway of degradation proposed. WB-grown culture filtrate decolorized and detoxified real and simulated textile effluents by about 40%. The study highlights the use of inexpensive materials for the production of enzymes effective on dyes and effluents.
Hongwei Liu - One of the best experts on this subject based on the ideXlab platform.
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three new cyathane diterpenoids from the medicinal fungus Cyathus africanus
Journal of Asian Natural Products Research, 2015Co-Authors: Junjie Han, Yuhui Chen, Lei Zhang, Li Bao, Feng Zhao, Weiku Zhang, Hongwei LiuAbstract:Three new cyathane diterpenoids, cyathin W (1), cyathin V (2), and cyathin T (3), were isolated from the solid culture of Cyathus africanus. The structures and configurations of these new compounds were elucidated on the basis of comprehensive spectroscopic analysis including 1D NMR, 2D NMR (HSQC, HMBC, NOESY), and HR-ESI-MS experiments. Compounds 1 and 3 showed moderate inhibition against nitric oxide production in lipopolysaccaride-activated macrophages with IC50 value of 80.07 and 88.87 μM, respectively. In cytotoxicity assay, compound 1 showed weak cytotoxicity against K562 cell line with IC50 value of 12.1 μM.
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production of bioactive cyathane diterpenes by a bird s nest fungus Cyathus gansuensis growing on cooked rice
Food Chemistry, 2014Co-Authors: Botao Wang, Junjie Han, Yuhui Chen, Hongwei LiuAbstract:Cyathane diterpenes are important bioactive substances produced by some edible and medicinal fungi. Seven new cyathane type diterpenes, named as cyathins J-P (1-7), together with two known diterpenes (8 and 9), were isolated from the solid culture of the bird's nest fungus Cyathus gansuensis growing on cooked rice. The structures of the new secondary metabolites were elucidated by NMR experiments. Bioactivity screening indicated that compounds 1, 2, 4, and 8 showed moderate inhibitory activity against NO production in lipopolysaccharide-activated macrophages with an IC50 value of 42, 78, 80, and 16 μM, respectively. The fungus C. gansuensis is a promising source of bioactive secondary metabolites and has application potential in preparing healthy food.
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isolation and identification of a new anti inflammatory cyathane diterpenoid from the medicinal fungus Cyathus hookeri berk
Fitoterapia, 2013Co-Authors: Sha Yan, Junjie Han, Yuhui Chen, Hongwei LiuAbstract:Abstract A new cyathane diterpene, named as cyathin I ( 1 ), as well as two known cyathane diterpenes (12R)-11a,14a-epoxy-13a,14b,15-trihydroxycyath-3-ene ( 2 ) and erinacine I ( 3 ), were isolated from the liquid culture of Cyathus hookeri Berk. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compounds 1 – 3 showed inhibition against nitric oxide production in macrophages with an IC 50 value of 15.5, 52.3, and 16.8 μM, respectively.
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anti inflammatory and cytotoxic cyathane diterpenoids from the medicinal fungus Cyathus africanus
Fitoterapia, 2013Co-Authors: Junjie Han, Yuhui Chen, Li Bao, Feng Zhao, Xiaoli Yang, Dailin Liu, Hongwei LiuAbstract:Abstract Five novel cyathane diterpenes, cyathins D–H (1–5), as well as three known diterpenes, neosarcodonin O (6), cyathatriol (7),and 11-O-acetylcyathatriol (8), were isolated from the solid culture of Cyathus africanus. The structures of the new compounds were elucidated by spectroscopic methods. The absolute configurations of compounds 2 and 8 were determined by single-crystal X-ray crystallographic analysis, whereas the absolute configuration of C-14 in 1 was determined via the circular dichroism data of the [Rh2(OCOCF3)4] complex. Compounds 3, 5, 6, 8, and 9 showed potent inhibition against nitric oxide production in lipopolysaccaride-activated macrophages with an IC50 value of 2.57, 1.45, 12.0, 10.73, and 9.45 μM, respectively. Compounds 6 and 8 showed strong cytotoxicity against Hela and K562 cell lines with the IC50 value less than 10 μM.
