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Marília Afonso Rabelo Buzalaf - One of the best experts on this subject based on the ideXlab platform.
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effects of ph and fluoride concentration of Dentifrices on fluoride levels in saliva biofilm and biofilm fluid in vivo
Clinical Oral Investigations, 2016Co-Authors: Karina Yuri Kondo, Alberto Carlos Botazzo Delbem, Marília Afonso Rabelo Buzalaf, M M Manarelli, Juliano Pelim PessanAbstract:Acidic Dentifrices have been shown to be more effective than neutral ones against dental caries using in vitro, in situ, and clinical protocols. Given the scarcity of studies assessing intraoral fluoride (F) retention after using such formulations, the present study evaluated the influence of pH and F concentration of Dentifrices on F uptake by saliva, biofilm, and biofilm fluid. Volunteers (n = 22) were randomly assigned to Dentifrices containing 0 (placebo), 550 (LFD, low-fluoride dentifrice), and 1100 ppm F (CD, conventional dentifrice) at pH 4.5 and 7.0 and brushed their teeth 3 times/day following a double-blind, crossover protocol. Saliva and biofilm samples were collected after 7 days of using the Dentifrices, 1 and approximately 12 h after last brushing. F and calcium (Ca) analyses were performed with the inverted electrode after buffering with TISAB III and the Arsenazo III method, respectively. Data were analyzed by 3-way ANOVA, Tukey’s HSD test, and Pearson’s correlation coefficient (p < 0.05). F concentrations in biofilm fluid and whole biofilm 1 h after brushing with acidic F-toothpastes were higher than those related to neutral counterparts, although the differences were small and not significant; no increases were observed in salivary F concentrations influenced by dentifrice pH. Moreover, no definite trend was observed for Ca concentrations in these compartments. Dentifrice pH had some influence on F uptake by the biofilm fluid, having lesser or no impact on F uptake by the biofilm and saliva, respectively. Toothbrushing with acidic toothpastes leads to slight increases in F concentrations in the biofilm fluid when compared to neutral formulations, which may contribute to the higher anticaries effect of acidic formulations.
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intraoral fluoride levels after use of conventional and high fluoride Dentifrices
Clinical Oral Investigations, 2015Co-Authors: Juliano Pelim Pessan, Larissa Tercilia Grizzo, Juliana Mendonca Da Conceicao, Melinda Szekely, Zita Fazakas, Marília Afonso Rabelo BuzalafAbstract:This study aimed to evaluate saliva and plaque as indicators of intraoral fluoride (F) levels after the use of conventional and high-fluoride Dentifrices. Subjects were randomly assigned to brush their teeth with conventional (1000 ppm F), high-fluoride (5000 ppm F), and placebo Dentifrices (fluoride free) for 10 days, following a double-blind, crossover protocol. Saliva and plaque samples were collected on the morning of the 5th and 10th days, respectively at 1 and 12 h after brushing, and analyzed with an ion-selective electrode after HMDS-facilitated diffusion. Data were analyzed by two-way repeated measures ANOVA, Tukey’s test and Spearman’s correlation coefficient (p < 0.05). Plaque and salivary F levels were significantly increased after the use of conventional and high-fluoride Dentifrices when compared to values obtained for placebo, except plaque 12 h after the use of conventional dentifrice. A positive and significant correlation was found between fluoride concentrations in plaque and saliva for both times of sample collection. Both indicators assessed were able to detect significant differences among treatments and between times after brushing. The use of a high-fluoride dentifrice is able to significantly increase intraoral fluoride levels throughout the day, being therefore a useful therapy for patients at high caries risk. A dentifrice with high fluoride concentration could be regarded as a useful therapy of F delivery for high caries-risk patients, since intraoral F levels were sustained throughout most of the day after using this formulation.
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distribution of fluoride and calcium in plaque biofilms after the use of conventional and low fluoride Dentifrices
International Journal of Paediatric Dentistry, 2014Co-Authors: Juliano Pelim Pessan, Irene Ramires, Flavia De Moraes Italiani, Gary M Whitford, Jose Roberto Pereira Lauris, K. M. R. P. Alves, K J Toumba, C Robinson, Marília Afonso Rabelo BuzalafAbstract:Background. The distribution of fluoride and calcium in plaque after the use of fluoride Dentifrices has not yet been determined. Aim. To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride Dentifrices. Design. Children ( n= 11, 8–10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) Dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 9 5 lm) for fluoride and calcium analysis. Sections 4 lm thick were used for image analysis and determination of biomass fraction. Results were analysed by ANOVA, Tukey’s test, and linear regression analysis (P < 0.05). Results. Fluoride and calcium were mostly located at the outer sections of biofilms for all Dentifrices tested, and these ions were directly correlated throughout most of biofilm’s sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions. The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms’ sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.
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use of Dentifrices to prevent erosive tooth wear harmful or helpful
Brazilian Oral Research, 2014Co-Authors: Ana Carolina Magalhaes, Annette Wiegand, Marília Afonso Rabelo BuzalafAbstract:Dental erosion is the loss of dental hard tissues caused by non-bacterial acids. Due to acid contact, the tooth surface becomes softened and more prone to abrasion from toothbrushing. Dentifrices containing different active agents may be helpful in allowing rehardening or in increasing surface resistance to further acidic or mechanical impacts. However, Dentifrices are applied together with brushing and, depending on how and when toothbrushing is performed, as well as the type of dentifrice and toothbrush used, may increase wear. This review focuses on the potential harmful and helpful effects associated with the use of Dentifrices with regard to erosive wear. While active ingredients like fluorides or agents with special anti-erosive properties were shown to offer some degree of protection against erosion and combined erosion/abrasion, the abrasive effects of Dentifrices may increase the surface loss of eroded teeth. However, most evidence to date comes from in vitro and in situ studies, so clinical trials are necessary for a better understanding of the complex interaction of active ingredients and abrasives and their effects on erosive tooth wear.
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effect of low fluoride acidic Dentifrices on dental remineralization
Brazilian Dental Journal, 2013Co-Authors: Fernanda Lourencao Brighenti, Marília Afonso Rabelo Buzalaf, Eliana Mitsue Takeshita, Camila De Oliveira Santana, Alberto Carlos Botazzo DelbemAbstract:This study evaluated the capacity of fluoride acidic Dentifrices (pH 4.5) to promote enamel remineralization using a pH cycling model, comparing them with a standard dentifrice (1,100 µgF/g). Enamel blocks had their surface polished and surface hardness determined (SH). Next, they were submitted to subsurface enamel demineralization and to post-demineralization surface hardness analysis. The blocks were divided into 6 experimental groups (n=10): placebo (without F, pH 4.5, negative control), 275, 412, 550, 1,100 µgF/g and a standard dentifrice (positive control). The blocks were submitted to pH cycling for 6 days and treatment with dentifrice slurries twice a day. After pH cycling, surface and cross-sectional hardness were assessed to obtain the percentage of surface hardness recovery (%SH
Yun Po Zhang - One of the best experts on this subject based on the ideXlab platform.
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effect of stannous fluoride and zinc phosphate dentifrice on dental plaque and gingivitis a randomized clinical trial with 6 month follow up
Journal of the American Dental Association, 2019Co-Authors: Dutmanee Seriwatanachai, Luis R Mateo, Evaristo Delgado, T Triratana, Petchart Kraivaphan, Cholticha Amaornchat, Amarpreet Sabharwal, Gregory Szewczyk, Maria Emanuel Ryan, Yun Po ZhangAbstract:Abstract Objective The objective of this study was to compare a stabilized stannous fluoride (SnF2) dentifrice with zinc phosphate (Colgate TotalSF) with SnF2 with zinc lactate and control fluoride Dentifrices for gingivitis and plaque control over a 6-month period. Methods A total of 135 adult participants were enrolled in this study. After randomization and blinding of examiners and patients, enrolled participants were provided instructions for use of assigned dentifrice. At 3 visits (0, 3, and 6 months), various gingival and plaque indexes were collected to determine the clinical efficacy of a stabilized SnF2 dentifrice. These results were compared with a SnF2 with zinc lactate dentifrice and with a control fluoride dentifrice. Results A total of 135 participants completed the study. All groups reported statistically significant reductions in gingival inflammation and improvement in plaque control at 3- and 6-month follow-up. Both SnF2 Dentifrices showed statistically significant reductions in all indexes compared with the control dentifrice (P Conclusions This study reports similar efficacy of a test dentifrice to a commercial SnF2-containing dentifrice for plaque control and reduction in gingival inflammation and provides supporting evidence that the test dentifrice maintains its clinical efficacy with change of formulation. Practical Implications This newly formulated SnF2 stabilized with zinc phosphate dentifrice may be of benefit to patients in controlling plaque biofilm and gingivitis.
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randomized clinical trial of the efficacy of Dentifrices containing 1 5 arginine an insoluble calcium compound and 1450 ppm fluoride over two years
The Journal of clinical dentistry, 2015Co-Authors: Yisi Zhong, Luis R Mateo, Xianjun Jiang, Hu Deyu, Boyce M Morrison, Yun Po ZhangAbstract:Objective A double blind, randomized, unsupervised, parallel-group clinical trial was conducted on over 5,500 children in Sichuan Province, China. This clinical trial compared the anti-caries efficacy of two test Dentifrices to that of a control dentifrice. Methods The test Dentifrices contained 1.5% arginine, 1450 ppm fluoride as sodium monofluorophosphate (MFP), and an insoluble calcium compound (either dicalcium phosphate or calcium carbonate). The positive control dentifrice contained 1450 ppm fluoride as sodium fluoride (NaF), in a silica base. The children were randomly assigned one of the toothpastes, and children residing in the same household were assigned the same dentifrice to use at home, twice a day. Results Three calibrated dentists examined the children at baseline, as well as after one and two years of product use. After one year of product use, there were no statistically significant differences among the three groups with respect to decayed, missing, and filled teeth (DMFT) or to decayed, missing, and filled surfaces (DMFS). After two years of product use, subjects in the two test groups using the Dentifrices containing 1.5% arginine, 1450 ppm fluoride as MFP, and an insoluble calcium compound had a statistically significant reduction in DMFT increments of 20.5% and in DMFS increments of 19.6% when compared to subjects in the group using the positive control dentifrice. After two years, there were no statistically significant differences with respect to DMFT or DMFS between the two groups using the Dentifrices containing 1.5% arginine, 1450 ppm fluoride as MFP, and an insoluble calcium compound. Conclusion The use of the two test Dentifrices demonstrated significant reductions in decayed, missing, and filled teeth and surfaces, however there was no statistically significant different between the two test Dentifrices clinically after two years of using the toothpastes. The results of this two-year clinical investigation support the conclusion that Dentifrices containing 1.5% arginine, an insoluble calcium compound, and 1450 ppm fluoride as MFP provide superior protection against caries lesion cavitation compared to a positive control dentifrice containing only 1450 ppm fluoride as NaF.
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the anti caries efficacy of a dentifrice containing 1 5 arginine and 1450 ppm fluoride as sodium monofluorophosphate assessed using quantitative light induced fluorescence qlf
Journal of Dentistry, 2013Co-Authors: Wei Yin, Yun Po Zhang, Diane Cummins, X Fan, Iain A Pretty, L R Mateo, R P EllwoodAbstract:Objective: To compare the efficacy of a new dentifrice containing 1.5% arginine, an insoluble calcium compound and 1450 ppm fluoride to arrest and reverse naturally occurring buccal caries lesions in children relative to a positive control dentifrice containing 1450 ppm fluoride alone. Study design: Participants from Chengdu, Sichuan Province, China tested three Dentifrices: a new dentifrice containing 1.5% arginine, an insoluble calcium compound, and 1450 ppm fluoride, as sodium monofluorophosphate, a positive control dentifrice containing 1450 ppm fluoride, as sodium fluoride, in a silica base, and a matched negative control dentifrice without arginine and fluoride. Quantitative Light-induced Fluorescence (QLF) was used to assess buccal caries lesions at baseline and after 3 and 6 months of product use. Results: 438 participants (initial age 9‐13 years (mean 11.1 0.78) and 48.6% female) completed the study. No adverse events attributable to the products were reported during the course of the study. The subject mean DQ (mm 2 %), representing lesion volume, was 27.26 at
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a clinical investigation using quantitative light induced fluorescence qlf of the anticaries efficacy of a dentifrice containing 1 5 arginine and 1450 ppm fluoride as sodium monofluorophosphate
The Journal of clinical dentistry, 2013Co-Authors: Wei Yin, Yun Po Zhang, Diane Cummins, X Fan, Iain A Pretty, L R Mateo, Y Feng, R P EllwoodAbstract:OBJECTIVE: The purpose of this study was to assess the ability of a new dentifrice containing arginine, an insoluble calcium compound, and fluoride to arrest or reverse naturally occurring buccal caries lesions measured using Quantitative Light-induced Fluorescence (QLF). METHODS: Three study groups used Dentifrices which contained 1) 1.5% arginine and 1450 ppm fluoride as sodium monofluorophosphate (experimental), 2) 1450 ppm fluoride as sodium monofluorophosphate (positive control), and 3) no fluoride (negative control). All three Dentifrices were formulated in the same calcium base. The study participants were from three schools in the city of Chengdu, Sichuan Province, China. A total of 446 of 450 recruited subjects completed the study. Of these, 147 were in the experimental, 148 in the positive control, and 151 in the negative control groups. The initial age of the children was 10-12 years (mean 11.4 +/- 0.54); 47.5% were female. RESULTS: Using QLF, assessments of buccal caries lesions were made at baseline and after three and six months of product use. For AQ, representing lesion volume, the baseline mean value for the three groups was 27.30, and at the three-month examination the mean values were 16.76, 19.25, and 25.89 for the experimental, positive, and negative control Dentifrices, respectively. This represents improvements from baseline of 38.6%, 29.5%, and 5.2%. At six months, the deltaQ values for the three groups were 13.46, 18.47, and 24.18, representing improvements from baseline of 50.7%, 32.3%, and 11.4%. For all QLF metrics, deltaF (loss of fluorescence), area, and deltaQ, the differences between the negative control and both the experimental and positive control groups were statistically significant (p < or = 0.01). The differences between the experimental and positive control groups attained statistical significance for deltaQ (p < or = 0.003) at the six-month examination. CONCLUSION: It is concluded that both of the fluoride-containing toothpastes are significantly better at arresting and reversing buccal caries lesions than the non-fluoride toothpaste. Furthermore, it is concluded that the new dentifrice containing arginine, an insoluble calcium compound, and fluoride provides significantly greater anticaries benefit than a dentifrice containing fluoride alone.
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extrinsic stain removal efficacy of a new dentifrice containing 0 3 triclosan 2 0 pvm ma copolymer 0 243 naf and specially designed silica for sensitivity relief and whitening benefits as compared to a dentifrice containing 0 3 triclosan 2 pvm ma cop
American Journal of Dentistry, 2011Co-Authors: Salim Nathoo, Luis R Mateo, Yun Po Zhang, Evaristo Delgado, William DevizioAbstract:PURPOSE: This single-center, double-blind, randomized, parallel-group clinical study was designed to investigate the extrinsic stain removal efficacy of a new antisensitivity dentifrice containing 0.3% triclosan, 2% polyvinylmethyl ether/maleic acid copolymer (PVM/MA copolymer), 0.243% NaF and a new silica specially-designed to occlude dentin tubules, relative to a Positive Control dentifrice and a Negative Control dentifrice. METHODS: 117 qualifying adults were stratified by baseline Lobene Stain Index scores and randomly assigned to brush twice daily using a soft-bristled toothbrush and one of three Dentifrices: (1) the Test Dentifrice; (2) a previously clinically proven dentifrice variant containing 0.3% triclosan, 2% PVM/MA copolymer, 0.243% NaF in a high cleaning silica base (Positive Control); and (3) a dentifrice containing 0.243% NaF in a silica base (Negative Control). Extrinsic stain area and stain intensity examinations were repeated after 3 and 6 weeks of product use. RESULTS: Relative to the Negative Control group, the Test group and the Positive Control group exhibited statistically significant improvements in mean Lobene composite stain scores after 3 weeks of product use (39.8% and 40.7% respectively) and after 6 weeks of product use (58.8% and 61.8% respectively). There were no statistically significant differences observed between the stain removal performance of the Test Dentifrice and the Positive Control Dentifrice after 3 and 6 weeks of product use.
R P Ellwood - One of the best experts on this subject based on the ideXlab platform.
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the anti caries efficacy of a dentifrice containing 1 5 arginine and 1450 ppm fluoride as sodium monofluorophosphate assessed using quantitative light induced fluorescence qlf
Journal of Dentistry, 2013Co-Authors: Wei Yin, Yun Po Zhang, Diane Cummins, X Fan, Iain A Pretty, L R Mateo, R P EllwoodAbstract:Objective: To compare the efficacy of a new dentifrice containing 1.5% arginine, an insoluble calcium compound and 1450 ppm fluoride to arrest and reverse naturally occurring buccal caries lesions in children relative to a positive control dentifrice containing 1450 ppm fluoride alone. Study design: Participants from Chengdu, Sichuan Province, China tested three Dentifrices: a new dentifrice containing 1.5% arginine, an insoluble calcium compound, and 1450 ppm fluoride, as sodium monofluorophosphate, a positive control dentifrice containing 1450 ppm fluoride, as sodium fluoride, in a silica base, and a matched negative control dentifrice without arginine and fluoride. Quantitative Light-induced Fluorescence (QLF) was used to assess buccal caries lesions at baseline and after 3 and 6 months of product use. Results: 438 participants (initial age 9‐13 years (mean 11.1 0.78) and 48.6% female) completed the study. No adverse events attributable to the products were reported during the course of the study. The subject mean DQ (mm 2 %), representing lesion volume, was 27.26 at
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a clinical investigation using quantitative light induced fluorescence qlf of the anticaries efficacy of a dentifrice containing 1 5 arginine and 1450 ppm fluoride as sodium monofluorophosphate
The Journal of clinical dentistry, 2013Co-Authors: Wei Yin, Yun Po Zhang, Diane Cummins, X Fan, Iain A Pretty, L R Mateo, Y Feng, R P EllwoodAbstract:OBJECTIVE: The purpose of this study was to assess the ability of a new dentifrice containing arginine, an insoluble calcium compound, and fluoride to arrest or reverse naturally occurring buccal caries lesions measured using Quantitative Light-induced Fluorescence (QLF). METHODS: Three study groups used Dentifrices which contained 1) 1.5% arginine and 1450 ppm fluoride as sodium monofluorophosphate (experimental), 2) 1450 ppm fluoride as sodium monofluorophosphate (positive control), and 3) no fluoride (negative control). All three Dentifrices were formulated in the same calcium base. The study participants were from three schools in the city of Chengdu, Sichuan Province, China. A total of 446 of 450 recruited subjects completed the study. Of these, 147 were in the experimental, 148 in the positive control, and 151 in the negative control groups. The initial age of the children was 10-12 years (mean 11.4 +/- 0.54); 47.5% were female. RESULTS: Using QLF, assessments of buccal caries lesions were made at baseline and after three and six months of product use. For AQ, representing lesion volume, the baseline mean value for the three groups was 27.30, and at the three-month examination the mean values were 16.76, 19.25, and 25.89 for the experimental, positive, and negative control Dentifrices, respectively. This represents improvements from baseline of 38.6%, 29.5%, and 5.2%. At six months, the deltaQ values for the three groups were 13.46, 18.47, and 24.18, representing improvements from baseline of 50.7%, 32.3%, and 11.4%. For all QLF metrics, deltaF (loss of fluorescence), area, and deltaQ, the differences between the negative control and both the experimental and positive control groups were statistically significant (p < or = 0.01). The differences between the experimental and positive control groups attained statistical significance for deltaQ (p < or = 0.003) at the six-month examination. CONCLUSION: It is concluded that both of the fluoride-containing toothpastes are significantly better at arresting and reversing buccal caries lesions than the non-fluoride toothpaste. Furthermore, it is concluded that the new dentifrice containing arginine, an insoluble calcium compound, and fluoride provides significantly greater anticaries benefit than a dentifrice containing fluoride alone.
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the effectiveness of a toothpaste containing triclosan and polyvinyl methyl ether maleic acid copolymer in improving plaque control and gingival health a systematic review
Journal of Clinical Periodontology, 2004Co-Authors: R M Davies, R P Ellwood, G M DaviesAbstract:Objective: To compare the effectiveness of triclosan/copolymer and fluoride Dentifrices in improving plaque control and gingival health. Search strategy: We searched the Cochrane Controlled Trials Register, MEDLINE (1986to March 2003) and EMBASE (1986 to March 2003). Personal files and the reference lists of all articles were checked for further studies. Selection criteria: Trials were selected if they met the following criteria: random allocation of participants; participants were adults with plaque and gingivitis; unsupervised use of Dentifrices for at least 6 months; and primary outcomes - plaque and gingivitis after 6 months. Data collection and analysis: Two reviewers independently extracted information. For each plaque and gingivitis index, the mean differences for each study were pooled as weighted mean differences (WMDs) with the appropriate 95% confidence intervals (CIs) using the random effect models. Main results: Sixteen trials provided data for the meta-analysis. The triclosan/ copolymer dentifrice significantly improved plaque control compared with a fluoride dentifrice, with a WMD of -0.48 (95% CI: - 0.64 to - 0.32) for the Quigley-Hein index and WMD of - 0.15 (95% CI: - 0.20 to - 0.09) for the plaque severity index. When compared with a fluoride dentifrice, the triclosan/copolymer dentifrice significantly reduced gingivitis with WMDs - 0.26 (95% CI: -0.34 to - 0.18) and - 0.12 (95% CI: - 0.17 to - 0.08) for the Loe and Silness index and gingivitis severity index, respectively.
Jaime Aparecido Cury - One of the best experts on this subject based on the ideXlab platform.
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ph cycling models to evaluate the effect of low fluoride dentifrice on enamel de and remineralization
Brazilian Dental Journal, 2008Co-Authors: Celso Silva Queiroz, Anderson T Hara, Adriana Franco Paes Leme, Jaime Aparecido CuryAbstract:Since the currently available pH-cycling models do not differentiate the anti-caries potential of Dentifrices with low fluoride (F)concentration, two models were developed and tested in the present. Bovine enamel blocks were subjected to the models and treat edwith F solutions containing from 70 to 280 µg F/mL in order to validate them in terms of dose-response effect. The models were alsotested by evaluating the Dentifrices Colgate Baby (500 µg F/g, as a low fluoride dentifrice), Tandy (1,100 µg F/g, as an active F-dentifrice) and Crest (1,100 µg F/g, as positive control). Enamel mineral loss or gain was assessed by surface and cross-sectionalmicrohardness, and lesion depth was analyzed by polarized light microscopy. The pH-cycling models showed F dose-response effecteither reducing enamel demineralization or enhancing remineralization. The low F dentifrice presented anti-caries potential, but it wasnot equivalent to the Dentifrices containing 1,100 µg F/g. These data suggest that the models developed in this study were able toevaluate the anti-caries potential of low F dentifrice either on resistance to demineralization or on enhancement of reminerali zation.Key Words: demineralization, remineralization, fluoride, dentifrice.
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ph cycling models to evaluate the effect of low fluoride dentifrice on enamel de and remineralization
Brazilian Dental Journal, 2008Co-Authors: Celso Silva Queiroz, Anderson T Hara, Adriana Franco Paes Leme, Jaime Aparecido CuryAbstract:Since the currently available pH-cycling models do not differentiate the anti-caries potential of Dentifrices with low fluoride (F) concentration, two models were developed and tested in the present. Bovine enamel blocks were subjected to the models and treated with F solutions containing from 70 to 280 mg F/mL in order to validate them in terms of dose-response effect. The models were also tested by evaluating the Dentifrices Colgate Baby (500 mg F/g, as a low fluoride dentifrice), Tandy (1,100 mg F/g, as an active F-dentifrice) and Crest (1,100 mg F/g, as positive control). Enamel mineral loss or gain was assessed by surface and cross-sectional microhardness, and lesion depth was analyzed by polarized light microscopy. The pH-cycling models showed F dose-response effect either reducing enamel demineralization or enhancing remineralization. The low F dentifrice presented anti-caries potential, but it was not equivalent to the Dentifrices containing 1,100 mg F/g. These data suggest that the models developed in this study were able to evaluate the anti-caries potential of low F dentifrice either on resistance to demineralization or on enhancement of remineralization.
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effect of triclosan Dentifrices on mouth volatile sulphur compounds and dental plaque trypsin like activity during experimental gingivitis development
Journal of Clinical Periodontology, 2002Co-Authors: Getulio Nogueirafilho, Poliana Mendes Duarte, Sergio De Toledo, Cinthia Pereira Machado Tabchoury, Jaime Aparecido CuryAbstract:Background: The objective of this study was to evaluate the effect of three commercial anti-plaque Dentifrices containing 0.3% triclosan + 2% pvm/ma (Colgate Total®), 0.3% triclosan + 0.75% Zn (Signal Global®) and 0.3% triclosan + 5% PPi (Crest Complete®) in comparison with an experimental dentifrice (0.3% triclosan + 2% pvm/ma + 0.75% Zn + 4% PPi) and a control dentifrice without anti-plaque agents on trypsin-like activity in dental plaque (detected by the hydrolysis of [Na-Benzoyl-DL-Anginine p-Nitroanilide (BAPNA)] and volatile sulphur compounds (VSCs) in mouth air during experimental gingivitis development. Method: A 5-step double blind, crossover experimental gingivitis study was conducted on 19 volunteers during a 21-day period. The volunteers refrained from brushing an experimental quadrant of teeth. The Dentifrices were applied to those teeth via toothshield three times per day; simultaneously they brushed the other teeth with the same dentifrice. After each period, VSCs in mouth air and BAPNA hydrolysis by dental plaque accumulated in the experimental quadrant were determined. Results: There was an increase (p < 0.05) in VSCs in mouth air when experimental gingivitis was induced in only one quadrant of teeth. None of the Dentifrices was able to avoid the increase of VSCs during the experimental gingivitis development. The majority of the antiplaque Dentifrices evaluated reduced the increase of VSC formation in comparison with the control (p < 0.05). There was no relationship between the ability of the Dentifrices in reducing VSC formation and the inhibition of trypsin-like activity in dental plaque. Conclusions: Anti-plaque Dentifrices reduce the increase of VSCs that occurs during the development of experimental gingivitis.
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effect of 3 Dentifrices containing triclosan and various additives an experimental gingivitis study
Journal of Clinical Periodontology, 2000Co-Authors: Getulio Nogueirafilho, Sergio De Toledo, Jaime Aparecido CuryAbstract:Background: The antiplaque and antigingivitis effect of 3 Dentifrices was evaluated using the 21-day partial-mouth experimental model of gingivitis. Method: 25 volunteers took part in this cross-over, double-blind study, carried out in 4 phases of 21 days each. For each phase of the study, a toothshield of the IV quadrant was constructed for each volunteer. 2 antiplaque Dentifrices from the market, one containing triclosan + pvm/ma and the other triclosan + Zn, were compared with an experimental formulation and its placebo. The experimental dentifrice contained triclosan + pvm/ma + Zn + PPi and the placebo (control) did not contain these substances. The subjects were stratified according to their whole-mouth baseline plaque (PI), gingivitis (GI) and bleeding (BI) index scores, and then randomly assigned to 1 of 4 Dentifrices. During each phase, while the volunteers brushed their teeth with one of the Dentifrices, the IV quadrant was protected by the toothshield filled with the dentifrice used. After each phase, dental plaque, gingivitis and bleeding indices were determined. Results: The results showed that only the formulation containing triclosan + pvm/ma + Zn + PPi was able to reduce PI (28.8%), GI (35.9%) and BI (30.4%) in comparison with controls (p<0.05). Conclusions: The data suggest that a dentifrice containing the combination of triclosan + pvm/ma + Zn + PPi should be formulated for dental plaque control.
Juliano Pelim Pessan - One of the best experts on this subject based on the ideXlab platform.
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effects of ph and fluoride concentration of Dentifrices on fluoride levels in saliva biofilm and biofilm fluid in vivo
Clinical Oral Investigations, 2016Co-Authors: Karina Yuri Kondo, Alberto Carlos Botazzo Delbem, Marília Afonso Rabelo Buzalaf, M M Manarelli, Juliano Pelim PessanAbstract:Acidic Dentifrices have been shown to be more effective than neutral ones against dental caries using in vitro, in situ, and clinical protocols. Given the scarcity of studies assessing intraoral fluoride (F) retention after using such formulations, the present study evaluated the influence of pH and F concentration of Dentifrices on F uptake by saliva, biofilm, and biofilm fluid. Volunteers (n = 22) were randomly assigned to Dentifrices containing 0 (placebo), 550 (LFD, low-fluoride dentifrice), and 1100 ppm F (CD, conventional dentifrice) at pH 4.5 and 7.0 and brushed their teeth 3 times/day following a double-blind, crossover protocol. Saliva and biofilm samples were collected after 7 days of using the Dentifrices, 1 and approximately 12 h after last brushing. F and calcium (Ca) analyses were performed with the inverted electrode after buffering with TISAB III and the Arsenazo III method, respectively. Data were analyzed by 3-way ANOVA, Tukey’s HSD test, and Pearson’s correlation coefficient (p < 0.05). F concentrations in biofilm fluid and whole biofilm 1 h after brushing with acidic F-toothpastes were higher than those related to neutral counterparts, although the differences were small and not significant; no increases were observed in salivary F concentrations influenced by dentifrice pH. Moreover, no definite trend was observed for Ca concentrations in these compartments. Dentifrice pH had some influence on F uptake by the biofilm fluid, having lesser or no impact on F uptake by the biofilm and saliva, respectively. Toothbrushing with acidic toothpastes leads to slight increases in F concentrations in the biofilm fluid when compared to neutral formulations, which may contribute to the higher anticaries effect of acidic formulations.
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intraoral fluoride levels after use of conventional and high fluoride Dentifrices
Clinical Oral Investigations, 2015Co-Authors: Juliano Pelim Pessan, Larissa Tercilia Grizzo, Juliana Mendonca Da Conceicao, Melinda Szekely, Zita Fazakas, Marília Afonso Rabelo BuzalafAbstract:This study aimed to evaluate saliva and plaque as indicators of intraoral fluoride (F) levels after the use of conventional and high-fluoride Dentifrices. Subjects were randomly assigned to brush their teeth with conventional (1000 ppm F), high-fluoride (5000 ppm F), and placebo Dentifrices (fluoride free) for 10 days, following a double-blind, crossover protocol. Saliva and plaque samples were collected on the morning of the 5th and 10th days, respectively at 1 and 12 h after brushing, and analyzed with an ion-selective electrode after HMDS-facilitated diffusion. Data were analyzed by two-way repeated measures ANOVA, Tukey’s test and Spearman’s correlation coefficient (p < 0.05). Plaque and salivary F levels were significantly increased after the use of conventional and high-fluoride Dentifrices when compared to values obtained for placebo, except plaque 12 h after the use of conventional dentifrice. A positive and significant correlation was found between fluoride concentrations in plaque and saliva for both times of sample collection. Both indicators assessed were able to detect significant differences among treatments and between times after brushing. The use of a high-fluoride dentifrice is able to significantly increase intraoral fluoride levels throughout the day, being therefore a useful therapy for patients at high caries risk. A dentifrice with high fluoride concentration could be regarded as a useful therapy of F delivery for high caries-risk patients, since intraoral F levels were sustained throughout most of the day after using this formulation.
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distribution of fluoride and calcium in plaque biofilms after the use of conventional and low fluoride Dentifrices
International Journal of Paediatric Dentistry, 2014Co-Authors: Juliano Pelim Pessan, Irene Ramires, Flavia De Moraes Italiani, Gary M Whitford, Jose Roberto Pereira Lauris, K. M. R. P. Alves, K J Toumba, C Robinson, Marília Afonso Rabelo BuzalafAbstract:Background. The distribution of fluoride and calcium in plaque after the use of fluoride Dentifrices has not yet been determined. Aim. To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride Dentifrices. Design. Children ( n= 11, 8–10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) Dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 9 5 lm) for fluoride and calcium analysis. Sections 4 lm thick were used for image analysis and determination of biomass fraction. Results were analysed by ANOVA, Tukey’s test, and linear regression analysis (P < 0.05). Results. Fluoride and calcium were mostly located at the outer sections of biofilms for all Dentifrices tested, and these ions were directly correlated throughout most of biofilm’s sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions. The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms’ sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.
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factors influencing fluoride ingestion from dentifrice by children
Community Dentistry and Oral Epidemiology, 2011Co-Authors: Claudia Ayumi Nakai Kobayashi, Juliano Pelim Pessan, Melina Rodrigues Belini, Flavia De Moraes Italiani, Adriana Regina Colombo Pauleto, Juliana Julianelli De Araujo, Vanessa Tessarolli, Larissa Tercilia Grizzo, Maria Aparecida De Andrade Moreira Machado, Marília Afonso Rabelo BuzalafAbstract:Kobayashi CAN, Belini MR, Italiani FM, Pauleto ARC, Julianelli de Araujo J, Tessarolli V, Grizzo LT, Pessan JP, Machado MAAM, Buzalaf MAR. Factors influencing fluoride ingestion from dentifrice by children. Community Dent Oral Epidemiol 2011; 39: 426–432. © 2011 John Wiley & Sons A/S Abstract – Objective: This study assessed the percentage of the amount of dentifrice loaded onto the toothbrush that is ingested by children, taking into account age, the amount of dentifrice used during toothbrushing, and the dentifrice flavor. Methods: The sample consisted of 155 children of both genders attending public kindergartens and schools in Bauru, Brazil, divided into 5 groups (n = 30–32) of children aged 2, 3, 4, 5 and 6 years old. The Dentifrices used were Sorriso™ (1219 ppm F, peppermint-flavored) and Tandy™ (959 ppm F, tutti-frutti-flavored). The assessment of fluoride intake from Dentifrices was carried out six times for each child, using 0.3, 0.6, and 1.2 g of each dentifrice, following a random, crossover distribution. Brushing was performed by the children or their parents/caregivers according to the home habits and under the observation of the examiner. Fluoride present in the expectorant and on toothbrush was analyzed with an ion-specific electrode after HMDS-facilitated diffusion. Fluoride ingestion was indirectly derived. Results were analyzed by 3-way repeated-measures anova and Tukey’s tests (P < 0.05) using the percent dentifrice ingested as response variable. Results: Age and percent dentifrice ingested for both Dentifrices, and the three amounts used were inversely related (P < 0.0001). Percent dentifrice ingested was significantly higher after the use of Tandy™ under all conditions of the study when compared with Sorriso™ (P < 0.0001). Significant differences were observed when brushing with 0.3 g when compared with 1.2 g, for both Dentifrices tested (P < 0.05). Conclusions: The results indicate that all variables tested must be considered in preventive measures aiming to reduce the amount of fluoride ingested by young children.
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Effects of Regular and Low-fluoride Dentifrices on Plaque Fluoride
Journal of Dental Research, 2010Co-Authors: Juliano Pelim Pessan, Irene Ramires, Gary M Whitford, Fabio Correia Sampaio, K. M. R. P. Alves, M. F. L. Taga, Marília Afonso Rabelo BuzalafAbstract:Previous studies have indicated that the use of low-fluoride Dentifrices could lead to proportionally higher plaque fluoride levels when compared with conventional Dentifrices. This double-blind, randomized, crossover study determined the effects of placebo, low-fluoride, and conventional Dentifrices on plaque fluoride concentrations ([F]) in children living in communities with 0.04, 0.72, and 3.36 ppm F in the drinking water. Children used the toothpastes twice daily, for 1 wk. Samples were collected 1 and 12 hrs after the last use of Dentifrices and were analyzed for fluoride and calcium. Similar increases were found 1 hr after the children brushed with low-fluoride (ca. 1.9 mmol F/kg) and conventional (ca. 2.4 mmol F/kg) Dentifrices in the 0.04- and 0.72-ppm-F communities. Despite the fact that the increases were less pronounced in the 3.36-ppm-F community, our results indicate that the use of a low-fluoride dentifrice promotes a proportionally higher increase in plaque [F] when compared with that achieved with a conventional dentifrice, based on dose-response considerations.