The Experts below are selected from a list of 30444 Experts worldwide ranked by ideXlab platform

Chaomin Cheng - One of the best experts on this subject based on the ideXlab platform.

  • point of care rna based Diagnostic Device for covid 19
    Diagnostics (Basel Switzerland), 2020
    Co-Authors: Ting Yang, Yung Chih Wang, Ching Fen Shen, Chaomin Cheng
    Abstract:

    At the end of 2019, the novel coronavirus disease (COVID-19), a fast-spreading respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was reported in Wuhan, China and has now affected over 123 countries globally [...].

  • Urinalysis for diaper-wearing elderly people using a combination of cotton-based Diagnostic Devices and smartphone-based image analysis
    Health Technology, 2019
    Co-Authors: Wei-hsuan Sung, Chaomin Cheng
    Abstract:

    Background: Urinary tract infection (UTI) is one of the most common bacterial infections, annually affecting 150 million people worldwide. The highest prevalence of UTI in the overall elderly population. However, practical problems have occurred in urine sampling in diaper-wearing elderly people. Our aim is to develop a new type of the in-vitro Diagnostic Devices based on “cotton”, which allows us to detect the nitrite concentrations in urine (i.e., biomarker for urinary tract infection). The combination of cotton-based Diagnostic Device and a smartphone-based image analysis would help the healthcare people early diagnose urinalysis-based diseases such as UTI, and improve the life quality in elderly populations around the world. Methods: Our Diagnostic Device integrated into diaper is made up with three primary parts: a test pad, a flow channel made by hydrophobic and hydrophilic cotton and plastic packaging substrates. The smartphone-based image analysis (a specific APP) solved recording issues including variances in either light or focal distance. The mean value was calculated and analyzed based on 8-bit grayscale using ImageJ software and defined as 255– [experiment zone intensity]. Results: The nitrite test color changes from colorless to purple, correlating positively to concentration (from 0.156 to 5 µM) in both test paper only and test pad integrated with diaper and the mean value is calculated and analyzed. Conclusions: Since this Device is inexpensive, portable, simple, structurally integrating with diapers and functionally integrated with the smartphone-based application, it would have a wild range potential of becoming a commercially viable Diagnostic system, in particular, for the daily healthcare of elderly people.

  • Cotton-based Diagnostic Devices
    Scientific reports, 2014
    Co-Authors: Shang-chi Lin, Min-yen Hsu, Chen-meng Kuan, Hsi-kai Wang, Chia-ling Chang, Fan-gang Tseng, Chaomin Cheng
    Abstract:

    A good Diagnostic procedure avoids wasting medical resources, is easy to use, resists contamination and provides accurate information quickly to allow for rapid follow-up therapies. We developed a novel Diagnostic procedure using a “cotton-based Diagnostic Device” capable of real-time detection, i.e., in vitro Diagnostics (IVD), which avoids reagent contamination problems common to existing biomedical Devices and achieves the abovementioned goals of economy, efficiency, ease of use and speed. Our research reinforces the advantages of an easy-to-use, highly accurate Diagnostic Device created from an inexpensive and readily available U.S. FDA-approved material (i.e., cotton as flow channel and chromatography paper as reaction zone) that adopts a standard calibration curve method in a buffer system (i.e., nitrite, BSA, urobilinogen and uric acid assays) to accurately obtain semi-quantitative information and limit the cross-contamination common to multiple-use tools. Our system, which specifically targets urinalysis Diagnostics and employs a multiple biomarker approach, requires no electricity, no professional training and is exceptionally portable for use in remote or home settings. This could be particularly useful in less industrialized areas.

  • Molecular-level dengue fever Diagnostic Devices made out of paper
    Lab on a chip, 2013
    Co-Authors: Shih-chun Yang, Da-jeng Yao, Jiann-hwa Chen, Chaomin Cheng
    Abstract:

    This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based Diagnostic Device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.

Changchun Liu - One of the best experts on this subject based on the ideXlab platform.

  • Smart cup: A minimally-instrumented, smartphone-based point-of-care molecular Diagnostic Device
    Sensors and Actuators, B: Chemical, 2016
    Co-Authors: Shih Chuan Liao, Haim H. Bau, Jinzhao Song, Michael G Mauk, H. Friedman, Sita Awasthi, Jing Peng, Changchun Liu
    Abstract:

    Nucleic acid amplification-based Diagnostics offer rapid, sensitive, and specific means for detecting and monitoring the progression of infectious diseases. However, this method typically requires extensive sample preparation, expensive instruments, and trained personnel. All of which hinder its use in resource-limited settings, where many infectious diseases are endemic. Here, we report on a simple, inexpensive, minimally-instrumented, smart cup platform for rapid, quantitative molecular Diagnostics of pathogens at the point of care. Our smart cup takes advantage of water-triggered, exothermic chemical reaction to supply heat for the nucleic acid-based, isothermal amplification. The amplification temperature is regulated with a phase-change material (PCM). The PCM maintains the amplification reactor at a constant temperature, typically, 60-65 °C, when ambient temperatures range from 12 to 35 °C. To eliminate the need for an optical detector and minimize cost, we use the smartphone's flashlight to excite the fluorescent dye and the phone camera to record real-time fluorescence emission during the amplification process. The smartphone can concurrently monitor multiple amplification reactors and analyze the recorded data. Our smart cup's utility was demonstrated by amplifying and quantifying herpes simplex virus type 2 (HSV-2) with LAMP assay in our custom-made microfluidic Diagnostic chip. We have consistently detected as few as 100 copies of HSV-2 viral DNA per sample. Our system does not require any lab facilities and is suitable for use at home, in the field, and in the clinic, as well as in resource-poor settings, where access to sophisticated laboratories is impractical, unaffordable, or nonexistent.

Shih Chuan Liao - One of the best experts on this subject based on the ideXlab platform.

  • Smart cup: A minimally-instrumented, smartphone-based point-of-care molecular Diagnostic Device
    Sensors and Actuators, B: Chemical, 2016
    Co-Authors: Shih Chuan Liao, Haim H. Bau, Jinzhao Song, Michael G Mauk, H. Friedman, Sita Awasthi, Jing Peng, Changchun Liu
    Abstract:

    Nucleic acid amplification-based Diagnostics offer rapid, sensitive, and specific means for detecting and monitoring the progression of infectious diseases. However, this method typically requires extensive sample preparation, expensive instruments, and trained personnel. All of which hinder its use in resource-limited settings, where many infectious diseases are endemic. Here, we report on a simple, inexpensive, minimally-instrumented, smart cup platform for rapid, quantitative molecular Diagnostics of pathogens at the point of care. Our smart cup takes advantage of water-triggered, exothermic chemical reaction to supply heat for the nucleic acid-based, isothermal amplification. The amplification temperature is regulated with a phase-change material (PCM). The PCM maintains the amplification reactor at a constant temperature, typically, 60-65 °C, when ambient temperatures range from 12 to 35 °C. To eliminate the need for an optical detector and minimize cost, we use the smartphone's flashlight to excite the fluorescent dye and the phone camera to record real-time fluorescence emission during the amplification process. The smartphone can concurrently monitor multiple amplification reactors and analyze the recorded data. Our smart cup's utility was demonstrated by amplifying and quantifying herpes simplex virus type 2 (HSV-2) with LAMP assay in our custom-made microfluidic Diagnostic chip. We have consistently detected as few as 100 copies of HSV-2 viral DNA per sample. Our system does not require any lab facilities and is suitable for use at home, in the field, and in the clinic, as well as in resource-poor settings, where access to sophisticated laboratories is impractical, unaffordable, or nonexistent.

Christof Strohhofer - One of the best experts on this subject based on the ideXlab platform.

  • A Novel Point of Care Diagnostic Device: Impedimetric Detection of a Biomarker in Whole Blood
    2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2007
    Co-Authors: Amanda A Mcmurray, Jan Kyselovik, Christopher A. Mills, Nicole Jaffrezic Renault, Hunor Santha, Christof Strohhofer
    Abstract:

    There is an unmet medical need for a more reliable and earlier assessment of patients suspected of having a deep vein thrombosis. We describe a novel approach which is developing a highly reliable, accurate, portable and handheld prototype medical Diagnostic Device to improve radically the speed, accuracy and reliability with which DVT and related blood clotting conditions can be assessed. The Device will measure whole blood concentration of D-dimer, a recognized biomarker of increased blood clotting activity, and through innovation in the development of a novel detection, measurement and reporting system, will offer the opportunity to use the test in the point of care setting. The Device combines innovation in antibody bio-engineering for high specificity immunoassay-based Diagnostics and nano/micro engineered impedimetric analysis electrodes incorporating a biocompatible polymer substrate with development of a disposable microfluidic manifold specifically enabling Diagnostics at the point-of-first-contact.

J A Kates - One of the best experts on this subject based on the ideXlab platform.

  • Regulatory hurdles in bringing an in vitro Diagnostic Device to market.
    Clinical chemistry, 1996
    Co-Authors: K M Smith, J A Kates
    Abstract:

    We discuss the hurdles that developers and manufacturers of in vitro Diagnostic Devices face in obtaining regulatory approval to market their products in the US. A thorough understanding of medical Device regulation and the early planning of a clinical and regulatory strategy are imperative in assuring successful and timely launches of new products. Finally, it is critical for manufacturers to establish a working partnership with the Food and Drug Administration to expedite their new product applications.