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Lucia Helena O’dwyer - One of the best experts on this subject based on the ideXlab platform.
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Hepatozoon milleri sp. nov. (Adeleorina: Hepatozoidae) in Akodon montensis (Rodentia: Cricetidae: Sigmodontinae) from southeastern Brazil.
Parasitology, 2018Co-Authors: Larissa De Castro Demoner, Maria Regina Lucas Da Silva, Natalia Mizuhira Magro, Lucia Helena O’dwyerAbstract:Members of the genus Hepatozoon (Miller, 1908) are blood parasites found in a wide range of host species, including wild rodents; however, information about the life cycle, distribution and Hepatozoon species diversity infecting these mammals are lacking. We studied the parasite stages and DNA sequences of Hepatozoon sp. of 11 naturally infected Akodon montensis. Thin blood smears, tissue samples and whole blood were obtained for morphology, morphometry and molecular analyses. Seven of the 11 rodents had gamonts on the blood smears. Biological and morphological features of the parasite such as tissue tropism, gamonts and meronts size and morphology, as well as the DNA sequence comparison and phylogenetic analysis, indicated that the Hepatozoon sp. detected in this study is distinct from those species previously reported in small rodents. Herein, we propose a new species, named Hepatozoon milleri sp. nov. This is the first description of a new Hepatozoon species from wild small rodents in Brazil, based on morphological and molecular characteristics.
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A survey of hemoparasites and ectoparasites in Nasua nasua Linnaeus, 1766 with a redescription of Hepatozoon procyonis Richards, 1961 based on morphological and molecular data.
Parasitology research, 2018Co-Authors: Maria Regina Lucas Da Silva, Felipe Fornazari, Carlos Roberto Teixeira, Alícia Giolo Hippólito, Jacqueline Muniz Bisca, Thiago F. Martins, Luna Scarpari Rolim, Lucia Helena O’dwyerAbstract:Hemoparasites are vector-borne parasites that infect wild carnivores worldwide. Since data on hemoparasite infections in Nasua nasua from Brazil are lacking, the aim of this study was to investigate the occurrence of hemoparasites and ectoparasites in N. nasua from different areas of Brazil. Blood samples and ectoparasites from 83 N. nasua were collected in Botucatu, Palmital, and Sao Paulo municipalities. Samples were screened via microscopy and molecular methods to detect hemoparasites. Tissues from two N. nasua were obtained for histopathological and molecular analyses. All 83 samples were negative for piroplasms on morphological and molecular examination. Thin blood smears of nine animals were positive for Hepatozoon gamonts. The gamonts shared morphological characteristics of Hepatozoon procyonis. Meronts were detected in the liver and spleen tissue of one animal. Twenty-one blood samples and four tissue samples were PCR positive for Hepatozoon sp. The sequences obtained were 97% identical to those of Hepatozoon felis, Hepatozoon ursi, and Hepatozoon sp. Based on searches for similarity and morphology, we identified the sequences as belonging to H. procyonis. This study provides epidemiological data on hemoparasite infections and redescribe H. procyonis based on morphological, morphometrical, and molecular analyses.
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Didelphis albiventris naturally infected with Hepatozoon canis in southeastern Brazil.
Ticks and tick-borne diseases, 2017Co-Authors: Maria Regina Lucas Da Silva, Felipe Fornazari, Larissa De Castro Demoner, Carlos Roberto Teixeira, Helio Langoni, Lucia Helena O’dwyerAbstract:Hepatozoon species are vector-borne pathogens that infect domestic and wild animals. Marsupials of the species Didelphis albiventris are adapted to urban and peri-urban areas and act as reservoir hosts for several parasites. The present study evaluated the occurrence of infection by Hepatozoon species in synantropic D. albiventris from Botucatu, Sao Paulo, Brazil. Blood samples and ectoparasites from 19 D. albiventris were collected from urban and peri-urban areas. Hepatozoon spp. detection was performed by microscopy and molecular analysis. One opossum was positive for Hepatozoon spp. in microscopy analysis and PCR, while another animal was positive only in PCR. The obtained sequences were 100% identical to Hepatozoon canis. Six species of ticks and two species of fleas were detected on D. albiventris. This is the first report of H. canis in synantropic D. albiventris. In Brazil, H. canis transmission among dog populations is not well established, which highlights the importance of investigating the role that opossums might play in the epidemiology of this protozoan.
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Seasonal variation of Hepatozoon spp. (Apicomplexa, Hepatozoidae) parasitemia from Boa constrictor amarali (Serpentes, Boidae) and Hydrodynastes gigas (Serpentes, Colubridae)
Parasitology Research, 2005Co-Authors: Mariana Morena De Vieira Santos, Lucia Helena O’dwyer, Reinaldo José Da SilvaAbstract:The aim of this study was to evaluate the parasitemia variation of three Hepatozoon species in Brazilian snakes. This study was conducted between 2001 and 2003 and included Hepatozoon terzii from Boa constrictor amarali , and Hepatozoon migonei and Hepatozoon cyclagrasi from Hydrodynastes gigas . It was observed that the parasitemia tended to decrease in all three Hepatozoon species but the parasites were not eliminated. This data suggest that Hepatozoon infection may be similar to Toxoplasma gondii infection, in that it persists throughout host life.
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Molecular identification and characterization of canine Hepatozoon species from Brazil
Parasitology Research, 2005Co-Authors: Adriano Stefani Rubini, Paulo Eduardo Martins Ribolla, Karina Dos Santos Paduan, Gustavo Góes Cavalcante, Lucia Helena O’dwyerAbstract:Canine Hepatozoon species from Brazil was molecular identified and characterized for the first time. From 31 dogs, 7 were positive for blood smear examination and 21 positive for PCR. Partial sequences of the 18S rRNA gene from eight naturally infected dogs were analyzed. Sequences revealed that Brazilian Hepatozoon is closely related with the Japanese Hepatozoon , that has 99% nucleotide identity with Hepatozoon canis from Israel, and different from Hepatozoon americanum . These results indicate that the canine Hepatozoon species from Brazil is H. canis .
Lucia Helena Odwyer - One of the best experts on this subject based on the ideXlab platform.
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Hepatozoon spp infect free ranging jaguars panthera onca in brazil
Journal of Parasitology, 2017Co-Authors: Mariana Malzoni Furtado, Lucia Helena Odwyer, Marcelo B Labruna, Thiago F. Martins, Karina Dos Santos Paduan, Betina Metzger, Anah Tereza De Almeida Jacomo, Grasiela Edith De Oliveira Porfirio, Leandro Silveira, Rahel SollmannAbstract:Abstract This study investigated the presence of Hepatozoon spp. in jaguars (Panthera onca) and domestic animals in the Cerrado, Amazon, and Pantanal biomes of Brazil. Between February 2000 and January 2010, blood samples were collected from 30 jaguars, 129 domestic dogs (Canis lupus familiaris), and 22 domestic cats (Felis catus) for molecular tests. All of the jaguars from the Pantanal (n = 22) and Cerrado (n = 4) and 3 of 4 jaguars from the Amazon were positive for Hepatozoon spp. Domestic dogs (62.8%) and cats (31.8%) were also positive for the agent. Hepatozoon nucleotide sequences from jaguars and domestic cats grouped with other Hepatozoon felis, whereas Hepatozoon from domestic dogs showed high similarity to Hepatozoon canis. Different species of Amblyomma were identified as parasitizing the jaguars and may act as vectors for Hepatozoon spp. Jaguars from the 3 sites were healthy and did not seem to be threatened by the hemoparasite within its population or environments. Most likely, jaguars play a...
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Hepatozoon spp infections in wild rodents in an area of endemic canine Hepatozoonosis in southeastern brazil
Ticks and Tick-borne Diseases, 2016Co-Authors: Larissa De Castro Demoner, Maria Regina Lucas Da Silva, Natalia Mizuhira Magro, Joao Marcelo Azevedo De Paula Antunes, Cecilia Calabuig, Lucia Helena OdwyerAbstract:Hepatozoon canis is a tick-borne parasite that occurs worldwide. In rural areas of Brazil, H. canis vectors remain unknown, which has led to speculation about alternative routes of transmission. Small rodents can play a role in the transmission (via predation) of Hepatozoon americanum, which led us to question whether predation might be an alternative mode of transmission for H. canis. Thus, this study investigated whether Hepatozoon spp. are present in wild small rodents in forest fragments that surround rural areas in Botucatu County, Sao Paulo, Brazil, where canine Hepatozoonosis is endemic. The study included blood samples from 158 dogs, which were screened by microscopy and molecular analysis. Blood samples and tissues from 67 rodents were obtained for histopathology and molecular detection. The prevalence of H. canis was high (66.45%) in dogs from rural areas of Botucatu, Sao Paulo, Brazil. The molecular analysis showed that wild rodent species in Brazil were infected with Hepatozoon spp. other than H. canis. Therefore, although the hypothesis that sylvatic rodents act as reservoirs for H. canis was not supported, the presence of monozoic cysts in the rodents suggests that, in addition to intermediate hosts, wild small rodents in Brazil might act as paratenic hosts of Hepatozoon spp. because they harbor infective stages for intermediate host predators.
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description of three new species of Hepatozoon apicomplexa hepatozoidae from rattlesnakes crotalus durissus terrificus based on molecular morphometric and morphologic characters
Experimental Parasitology, 2013Co-Authors: Lucia Helena Odwyer, Reinaldo José Silva, Tatiana Cristina Moço, Karina Dos Santos Paduan, Carine Spenassatto, Paulo Eduardo Martins RibollaAbstract:Hepatozoon spp. are commonly found infecting snakes. Since the latter are parasitized by diverse forms and data in the literature show divergence, we studied Hepatozoon spp. diversity on Crotalus durissus terrificus snakes using both molecular and morphological approaches. Naturally infected animals were employed. Blood was collected, blood smears were prepared and an aliquot was stored at -20°C for DNA extraction. Five specimens of C. durissus terrificus were selected, each of them infected with one gamont type. Morphological and morphometric analyses of the found gamonts led to their grouping into three populations. For molecular characterization, seven oligonucleotide pairs that amplify distinct regions of rDNA gene were tested by adopting the PCR technique. Only the oligonucleotide pairs HepF300/Hep900 and HEMO1/HEMO2 were efficient in amplifying and distinguishing different isolates of Hepatozoon spp. from snakes. The better results were obtained when both oligonucleotide pairs were used in association. Based on the molecular and morphologic differences, three new species were proposed: Hepatozoon cuestensis sp. nov.; Hepatozoon cevapii sp. nov. and Hepatozoon massardii sp. nov. This is the first description of new Hepatozoon species from snakes, based on molecular characterization and morphological data, in South America.
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oocysts of Hepatozoon canis in rhipicephalus boophilus microplus collected from a naturally infected dog
Veterinary Parasitology, 2011Co-Authors: Renata Lima De Miranda, Antonio Vicente Mundim, Jacqueline Ribeiro De Castro, Maria Marlene Martins Olegario, Marcelo Emilio Beletti, Lucia Helena Odwyer, Osnat Eyal, Dalit Talmifrank, Marcia Cristina CuryAbstract:Canine Hepatozoonosis is a tick-borne disease caused by protozoans of the genus Hepatozoon. Several tick species have been implicated as potential vectors. Therefore, extensive studies are needed to determine the ‘natural’ endemic cycle of this parasite. This paper presents the first report of the presence of Hepatozoon canis oocysts in Rhipicephalus (Boophilus) microplus collected from an infected dog.
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molecular characterization of feline Hepatozoon species from brazil
Veterinary Parasitology, 2006Co-Authors: Adriano Stefani Rubini, Paulo Eduardo Martins Ribolla, Karina Dos Santos Paduan, Regina Raquel Perez, Lucia Helena OdwyerAbstract:Feline Hepatozoon species from Brazil was molecular identified and characterized for the first time in Sao Paulo state, Brazil. Partial sequences of the 18S rRNA gene from the Hepatozoon from three naturally infected cats were analyzed. Sequences revealed that feline Hepatozoon was closely related to the canine Hepatozoon canis from Brazil.
Nico J Smit - One of the best experts on this subject based on the ideXlab platform.
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monophyly of the species of Hepatozoon adeleorina hepatozoidae parasitizing african anurans with the description of three new species from hyperoliid frogs in south africa
Parasitology, 2017Co-Authors: Edward C Netherlands, Courtney A Cook, Louis H Du Preez, Maarten P M Vanhove, Luc Brendonck, Nico J SmitAbstract:Haemogregarines (Apicomplexa: Adeleiorina) are a diverse group of haemoparasites reported from almost all vertebrate classes. The most commonly recorded haemogregarines to parasitize anurans are species of Hepatozoon Miller, 1908. To date 16 Hepatozoon species have been described from anurans in Africa, with only a single species, Hepatozoon hyperolli (Hoare, 1932), infecting a member of the Hyperoliidae. Furthermore, only two Hepatozoon species are known from South African anurans, namely Hepatozoon theileri (Laveran, 1905) and Hepatozoon ixoxo Netherlands, Cook and Smit, 2014, from Amietia delalandii (syn. Amietia quecketti) and three Sclerophrys species, respectively. Blood samples were collected from a total of 225 individuals representing nine hyperoliid species from several localities throughout northern KwaZulu-Natal, South Africa. Twenty frogs from three species were found positive for haemogregarines, namely Afrixalus fornasinii (6/14), Hyperolius argus (2/39), and Hyperolius marmoratus (12/74). Based on morphological characteristics, morphometrics and molecular findings three new haemogregarine species, Hepatozoon involucrum Netherlands, Cook and Smit n. sp., Hepatozoon tenuis Netherlands, Cook and Smit n. sp. and Hepatozoon thori Netherlands, Cook and Smit n. sp., are described from hyperoliid hosts. Furthermore, molecular analyses show anuran Hepatozoon species to be a separate monophyletic group, with species isolated from African hosts forming a monophyletic clade within this cluster.
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redescription and molecular diagnosis of Hepatozoon theileri laveran 1905 apicomplexa adeleorina hepatozoidae infecting amietia quecketti anura pyxicephalidae
Folia Parasitologica, 2014Co-Authors: Edward C Netherlands, Courtney A Cook, Nico J Smit, Louis H Du PreezAbstract:Blood smears prepared from the peripheral blood of 20 wild caught Amietia quecketti (Boulenger) from the North-West University Botanical Gardens, North West Province, South Africa, were examined for the presence of haemogregarines. A haemogregarine species comparative in morphology, host and geographical locality to that of Haemogregarina theileri Laveran, 1905 was detected. The original description of H. theileri was based solely on frog peripheral blood gamont stages. Later, further parasite stages, including trophozoites and merogonic liver stages, were recorded in a related Amietia sp. from equatorial Africa. This species was originally classified as a member of the genus Haemogregarina Danilewsky, 1885, but due to the close life cycle and morphological resemblance to those of Hepatozoon species, H. theileri was later transferred from Haemogregarina to Hepatozoon Miller, 1908. In the present study, meront and merozoite stages not described before, along with previously observed trophozoite, immature and mature gamont stages, are described from the peripheral blood of hosts. In addition, comparative phylogenetic analysis of the partial 18S rDNA sequence of Hepatozoon theileri to those of other haemogregarine species, including those of species of Hepatozoon and a Haemogregarina, support the taxonomic transfer of H. theileri to Hepatozoon, nesting H. theileri within a clade comprising species parasitising other amphibians. This is the first molecular and phylogenetic analysis of an African anuran species of Hepatozoon.
Edward C Netherlands - One of the best experts on this subject based on the ideXlab platform.
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monophyly of the species of Hepatozoon adeleorina hepatozoidae parasitizing african anurans with the description of three new species from hyperoliid frogs in south africa
Parasitology, 2017Co-Authors: Edward C Netherlands, Courtney A Cook, Louis H Du Preez, Maarten P M Vanhove, Luc Brendonck, Nico J SmitAbstract:Haemogregarines (Apicomplexa: Adeleiorina) are a diverse group of haemoparasites reported from almost all vertebrate classes. The most commonly recorded haemogregarines to parasitize anurans are species of Hepatozoon Miller, 1908. To date 16 Hepatozoon species have been described from anurans in Africa, with only a single species, Hepatozoon hyperolli (Hoare, 1932), infecting a member of the Hyperoliidae. Furthermore, only two Hepatozoon species are known from South African anurans, namely Hepatozoon theileri (Laveran, 1905) and Hepatozoon ixoxo Netherlands, Cook and Smit, 2014, from Amietia delalandii (syn. Amietia quecketti) and three Sclerophrys species, respectively. Blood samples were collected from a total of 225 individuals representing nine hyperoliid species from several localities throughout northern KwaZulu-Natal, South Africa. Twenty frogs from three species were found positive for haemogregarines, namely Afrixalus fornasinii (6/14), Hyperolius argus (2/39), and Hyperolius marmoratus (12/74). Based on morphological characteristics, morphometrics and molecular findings three new haemogregarine species, Hepatozoon involucrum Netherlands, Cook and Smit n. sp., Hepatozoon tenuis Netherlands, Cook and Smit n. sp. and Hepatozoon thori Netherlands, Cook and Smit n. sp., are described from hyperoliid hosts. Furthermore, molecular analyses show anuran Hepatozoon species to be a separate monophyletic group, with species isolated from African hosts forming a monophyletic clade within this cluster.
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redescription and molecular diagnosis of Hepatozoon theileri laveran 1905 apicomplexa adeleorina hepatozoidae infecting amietia quecketti anura pyxicephalidae
Folia Parasitologica, 2014Co-Authors: Edward C Netherlands, Courtney A Cook, Nico J Smit, Louis H Du PreezAbstract:Blood smears prepared from the peripheral blood of 20 wild caught Amietia quecketti (Boulenger) from the North-West University Botanical Gardens, North West Province, South Africa, were examined for the presence of haemogregarines. A haemogregarine species comparative in morphology, host and geographical locality to that of Haemogregarina theileri Laveran, 1905 was detected. The original description of H. theileri was based solely on frog peripheral blood gamont stages. Later, further parasite stages, including trophozoites and merogonic liver stages, were recorded in a related Amietia sp. from equatorial Africa. This species was originally classified as a member of the genus Haemogregarina Danilewsky, 1885, but due to the close life cycle and morphological resemblance to those of Hepatozoon species, H. theileri was later transferred from Haemogregarina to Hepatozoon Miller, 1908. In the present study, meront and merozoite stages not described before, along with previously observed trophozoite, immature and mature gamont stages, are described from the peripheral blood of hosts. In addition, comparative phylogenetic analysis of the partial 18S rDNA sequence of Hepatozoon theileri to those of other haemogregarine species, including those of species of Hepatozoon and a Haemogregarina, support the taxonomic transfer of H. theileri to Hepatozoon, nesting H. theileri within a clade comprising species parasitising other amphibians. This is the first molecular and phylogenetic analysis of an African anuran species of Hepatozoon.
Paulo Eduardo Martins Ribolla - One of the best experts on this subject based on the ideXlab platform.
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description of three new species of Hepatozoon apicomplexa hepatozoidae from rattlesnakes crotalus durissus terrificus based on molecular morphometric and morphologic characters
Experimental Parasitology, 2013Co-Authors: Lucia Helena Odwyer, Reinaldo José Silva, Tatiana Cristina Moço, Karina Dos Santos Paduan, Carine Spenassatto, Paulo Eduardo Martins RibollaAbstract:Hepatozoon spp. are commonly found infecting snakes. Since the latter are parasitized by diverse forms and data in the literature show divergence, we studied Hepatozoon spp. diversity on Crotalus durissus terrificus snakes using both molecular and morphological approaches. Naturally infected animals were employed. Blood was collected, blood smears were prepared and an aliquot was stored at -20°C for DNA extraction. Five specimens of C. durissus terrificus were selected, each of them infected with one gamont type. Morphological and morphometric analyses of the found gamonts led to their grouping into three populations. For molecular characterization, seven oligonucleotide pairs that amplify distinct regions of rDNA gene were tested by adopting the PCR technique. Only the oligonucleotide pairs HepF300/Hep900 and HEMO1/HEMO2 were efficient in amplifying and distinguishing different isolates of Hepatozoon spp. from snakes. The better results were obtained when both oligonucleotide pairs were used in association. Based on the molecular and morphologic differences, three new species were proposed: Hepatozoon cuestensis sp. nov.; Hepatozoon cevapii sp. nov. and Hepatozoon massardii sp. nov. This is the first description of new Hepatozoon species from snakes, based on molecular characterization and morphological data, in South America.
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molecular characterization of feline Hepatozoon species from brazil
Veterinary Parasitology, 2006Co-Authors: Adriano Stefani Rubini, Paulo Eduardo Martins Ribolla, Karina Dos Santos Paduan, Regina Raquel Perez, Lucia Helena OdwyerAbstract:Feline Hepatozoon species from Brazil was molecular identified and characterized for the first time in Sao Paulo state, Brazil. Partial sequences of the 18S rRNA gene from the Hepatozoon from three naturally infected cats were analyzed. Sequences revealed that feline Hepatozoon was closely related to the canine Hepatozoon canis from Brazil.
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Molecular identification and characterization of canine Hepatozoon species from Brazil
Parasitology Research, 2005Co-Authors: Adriano Stefani Rubini, Paulo Eduardo Martins Ribolla, Karina Dos Santos Paduan, Gustavo Góes Cavalcante, Lucia Helena O’dwyerAbstract:Canine Hepatozoon species from Brazil was molecular identified and characterized for the first time. From 31 dogs, 7 were positive for blood smear examination and 21 positive for PCR. Partial sequences of the 18S rRNA gene from eight naturally infected dogs were analyzed. Sequences revealed that Brazilian Hepatozoon is closely related with the Japanese Hepatozoon , that has 99% nucleotide identity with Hepatozoon canis from Israel, and different from Hepatozoon americanum . These results indicate that the canine Hepatozoon species from Brazil is H. canis .