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Heikki Kallio - One of the best experts on this subject based on the ideXlab platform.

  • postprandial hyperglycemia and insulin response are affected by sea buckthorn Hippophae rhamnoides ssp turkestanica berry and its ethanol soluble metabolites
    European Journal of Clinical Nutrition, 2010
    Co-Authors: Hennamaria Lehtonen, Riikka Jarvinen, Kaisa M Linderborg, Matti Viitanen, Mika Venojarvi, H Alanko, Heikki Kallio
    Abstract:

    Postprandial hyperglycemia and insulin response are affected by sea buckthorn ( Hippophae rhamnoides ssp. turkestanica ) berry and its ethanol-soluble metabolites

  • inositols and methylinositols in sea buckthorn Hippophae rhamnoides berries
    Journal of Chromatography B, 2009
    Co-Authors: Heikki Kallio, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Marika Lassila, Eila Jarvenpaa
    Abstract:

    Abstract Sea buckthorn (Hippophae rhamnoides L.) berries, especially of ssp. sinensis, contain significant quantities of an unknown, water-soluble compound, evidently a cyclitol derivative. The compound was isolated by HPLC and analyzed by GC–MS [trimethylsilyl (TMS) derivative, selected ion monitoring (SIM) and total ion chromatogram (TIC) analyses], by 1H and 13C NMR and by optical activity measurements. The results together with analyses of reference compound verified the unambiguous structure (−)-2-O-methyl- l -chiro-inositol ( l -quebrachitol). In addition, chiro-inositol and myo-inositol existing in trace amounts were identified based on reference compounds, chromatographic data and mass spectra of the TMS derivatives. Methyl-myo-inositol was tentatively identified based on chromatography and mass spectrometry. Inositols and methyl inositols are bioactive compounds essential for regulating physiological processes of plants and humans. To our knowledge, this is the first report on the presence of chiro-inositol and myo-inositol in sea buckthorn and l -quebrachitol in edible berries. The identification of the inositols and l -quebrachitol in sea buckthorn may bring new insights into the sensory properties and also mechanisms behind the health effects of the berry.

  • fast analysis of sugars fruit acids and vitamin c in sea buckthorn Hippophae rhamnoides l varieties
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Katja Tiitinen, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Heikki Kallio
    Abstract:

    A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography...

  • quality components of sea buckthorn Hippophae rhamnoides varieties
    Journal of Agricultural and Food Chemistry, 2005
    Co-Authors: Katja Tiitinen, Mari Hakala, Heikki Kallio
    Abstract:

    The sensory quality and chemical constituents of juices from seven sea buckthorn (Hippophae rhamnoides L.) varieties were studied in two consecutive seasons. The juices were generally described as sour and astringent, with low sweetness and fruity flavor. The differences in sensory quality as well as in chemical composition between samples and years were significant (p < 0.05) in most parameters studied. The Chuiskaya variety was described as the sweetest, with the strongest fruity flavor, whereas the varieties Avgustinka, Botanicheskaya, Trofimovskaya, and Raisa were the sourest and most astringent. Total sugar (fructose and glucose) varied from 1.9 to 7.1 g/100 mL in juice, total acid (malic and quinic acids) from 3.1 to 5.1 g/100 mL, vitamin C from 29 to 176 mg/100 mL, and pulp oil from 0.7 to 3.6%. The soluble solids were between 7.4 and 12.6, the pH between 2.7 and 2.9, and the titrable acidity between 2.0 and 3.7. The redness was highest on Avgustinka and Raisa, but there were no differences in yell...

  • effects of sea buckthorn Hippophae rhamnoides l seed and pulp oils on experimental models of gastric ulcer in rats
    Fitoterapia, 2002
    Co-Authors: Jianfeng Xing, Baoru Yang, Yaling Dong, Bingwen Wang, Junxian Wang, Heikki Kallio
    Abstract:

    Oils from sea buckthorn (Hippophae rhamnoides L.) seeds and berries have traditionally been used in the treatment of disorders of skin and mucosa in China. Compared with the negative control, oral administration of CO(2)-extracted seed and pulp oils, 7.0 ml x kg(-1) x day(-1) significantly reduced ulcer formation in water-immersion (P < 0.05) and reserpine-induced (P < 0.01) models in rats. In addition, administration of the two oils, 3.5 ml x kg(-1) x day(-1), significantly reduced the index of pylorus ligation-induced gastric ulcer (P < 0.05) and sped up the healing process of acetic acid-induced gastric ulcer (P < 0.01). The results suggested that the CO(2)-extracted sea buckthorn seed and pulp oils have both preventive and curative effects against experimental gastric ulcers in rats.

R C Sawhney - One of the best experts on this subject based on the ideXlab platform.

  • effect of seabuckthorn Hippophae rhamnoides flavone on immune system an in vitro approach
    Phytotherapy Research, 2008
    Co-Authors: K P Mishra, Sudipta Chanda, Dev Karan, Lilly Ganju, R C Sawhney
    Abstract:

    There are several reports, which suggest that the consumption of foods rich in flavonoids is associated with a lower incidence of certain degenerative diseases, including cardiovascular disease. Flavones, of Seabuckthorn (SBT) (Hippophae rhamnoides L.) fruit berry can modulate the production and level of several signaling molecules associated with immune function and inflammation in vitro, including several cytokines. We have evaluated the immunomodulatory activity of ethanolic solution of SBT flavone (FLV) in human peripheral blood mononuclear cells (PBMCs). The SBT flavone was found to stimulate production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in PBMCs. However, increased expressions of p-IκB, NF-κ κ κ κB, and p-p38 were found in flavone-treated human PBMCs with significantly suppressed expression of CD25 (IL-2R). There was no alteration found in the nitric oxide (NO) production in mouse macrophage cell line RAW 264.7. These observations suggest that stimulation of IL-6 and TNF-alpha secretion may contribute to the putative beneficial effects of dietary flavone against microbial infection. Copyright © 2008 John Wiley & Sons, Ltd.

  • effect of Hippophae rhamnoides leaf extract against dengue virus infection in human blood derived macrophages
    Phytomedicine, 2008
    Co-Authors: Monika Jain, K P Mishra, Sudipta Chanda, Dev Karan, Lilly Ganju, A Katiyal, Y S Padwad, K M S Yogendra, R C Sawhney
    Abstract:

    Dengue virus occurs as four distinct serotypes, called Dengue 1, 2, 3, and 4. Symptomatic dengue virus infection ranges from a self limited febrile illness, dengue fever (DF), to a more severe disease, dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The anti-Dengue treatment is severely hampered as no specific therapeutic agents are available. Even present treatment strategies for Dengue are more supportive than curative. In the present study anti-dengue activity of Hippophae rhamnoides (Seabuckthorn, SBT) leaf extract was evaluated in Dengue virus type-2 infected blood-derived human macrophages as macrophages are the primary target of Dengue virus infection. Infected cells were treated with SBT leaf extract and compared with commercially available anti-viral drug, Ribavirin. The extract was able to maintain the cell viability of Dengue-infected cells at par with Ribavirin along with the decrease and increase in TNF-alpha and IFN-gamma respectively. Anti-dengue activity of SBT extract was further determined by the traditional plaque assay. These observations suggest that the SBT leaf extract has a significant anti-dengue activity and has the potential for the treatment of Dengue.

  • adaptogenic and safety evaluation of seabuckthorn Hippophae rhamnoides leaf extract a dose dependent study
    Food and Chemical Toxicology, 2007
    Co-Authors: Shalini Saggu, H M Divekar, R C Sawhney, Vanita Gupta, P. K. Banerjee, Rajesh Kumar
    Abstract:

    Abstract The effects of seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae), leaf aqueous extract were examined in rats for its adaptogenic activity and toxicity. Dose dependent adaptogenic study of extract was carried out at different doses administered orally, 30 min prior to cold (5 °C)–hypoxia (428 mmHg)–restraint (C–H–R) exposure. After sub-acute toxicity studies on 10 and 20 times doses of maximal effective dose administered for 14 days (single oral dose of 1 g/kg and 2 g/kg once daily) and maximal effective dose administered for 30 days (single oral dose of 100 mg/kg once daily), biochemical and hematological parameters were studied in the serum and blood. The maximal effective adaptogenic dose of the extract was 100 mg/kg body weight. No significant changes were observed in organ weight/body weight ratios, of any vital organ studied (except liver and kidney in 1 g/kg and 2 g/kg body weight doses, respectively), and biochemical and hematological parameters of the sub-acute drug treated animals in comparison to control rats. In acute toxicity study LD50 of the extract was observed to be >10 g/kg when given orally. These results indicate that seabuckthorn leaf aqueous extract possess potent adaptogenic activity with no toxicity even after sub-acute (30 days) maximal effective dose administration.

  • adaptogenic and safety evaluation of seabuckthorn Hippophae rhamnoides leaf extract a dose dependent study
    Food and Chemical Toxicology, 2007
    Co-Authors: Shalini Saggu, H M Divekar, R C Sawhney, Vanita Gupta, P. K. Banerjee, Ratan Kumar
    Abstract:

    The effects of seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae), leaf aqueous extract were examined in rats for its adaptogenic activity and toxicity. Dose dependent adaptogenic study of extract was carried out at different doses administered orally, 30min prior to cold (5 degrees C)-hypoxia (428mmHg)-restraint (C-H-R) exposure. After sub-acute toxicity studies on 10 and 20 times doses of maximal effective dose administered for 14 days (single oral dose of 1g/kg and 2g/kg once daily) and maximal effective dose administered for 30 days (single oral dose of 100mg/kg once daily), biochemical and hematological parameters were studied in the serum and blood. The maximal effective adaptogenic dose of the extract was 100mg/kg body weight. No significant changes were observed in organ weight/body weight ratios, of any vital organ studied (except liver and kidney in 1g/kg and 2g/kg body weight doses, respectively), and biochemical and hematological parameters of the sub-acute drug treated animals in comparison to control rats. In acute toxicity study LD(50) of the extract was observed to be >10g/kg when given orally. These results indicate that seabuckthorn leaf aqueous extract possess potent adaptogenic activity with no toxicity even after sub-acute (30 days) maximal effective dose administration.

  • influence of sea buckthorn Hippophae rhamnoides l flavone on dermal wound healing in rats
    Molecular and Cellular Biochemistry, 2006
    Co-Authors: Asheesh Gupta, P. K. Banerjee, Virendra Singh, Ratan Kumar, Karan Pal, R C Sawhney
    Abstract:

    The present investigation was undertaken to determine the efficacy of topical administration of flavone of sea buckthorn (Hippophae rhamnoides L.) on cutaneous wound healing in rats. Four full-thickness excision wounds were created on the back of rat and 1.0% w/v flavone prepared in propylene glycol was applied topically. Control animals received the vehicle alone in an identical manner. The healing of the wound was assessed by the rate of wound contraction, period of epithelialization, hydroxyproline, hexosamine, antioxidants estimation and histopathology of the granulation tissue. The sea buckthorn flavone promoted the wound healing activity as indicated by improved rate of wound contraction, decreased time taken for epithelialization (16.3 days versus 24.8 days in controls) and significant increase in hydroxyproline (26.0%) and hexosamine (30.0%) content. These findings were also confirmed by histopathological examinations. In addition, it was observed that sea buckthorn flavone possesses potent antioxidant properties as evidenced by significant increase in reduced glutathione (55.0%), vitamin C (70.0%) and catalase (20.0%) activities in wound granulation tissue. The flavone treatment also resulted in significant decrease in lipid peroxide levels (39.0%). The results suggest that the sea buckthorn flavone promotes wound healing activity.

Honglun Wang - One of the best experts on this subject based on the ideXlab platform.

  • molecular sex identification in dioecious Hippophae rhamnoides l via rapd and scar markers
    Molecules, 2018
    Co-Authors: Wu Zhou, Honglun Wang, Yuwei Wang, Gong Zhang, Guangxiang Luan, Shasha Chen, Jing Meng, Yourui Suo
    Abstract:

    The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.

  • Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers
    MDPI AG, 2018
    Co-Authors: Wu Zhou, Honglun Wang, Yuwei Wang, Gong Zhang, Guangxiang Luan, Shasha Chen, Jing Meng, Yourui Suo
    Abstract:

    The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications

  • Isolation and structure determination of a new flavone glycoside from seed residues of seabuckthorn (Hippophae rhamnoides L.).
    Natural product research, 2017
    Co-Authors: Wenna Zhou, Zhenzhen Yuan, Ouyang Jian, Yourui Suo, Honglun Wang
    Abstract:

    In this study, a valid method was established for the isolation and purification of flavone glycosides from Hippophae rhamnoides L. seed residues using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-methanol-n-butyl alcohol-water (9:1:0.5:9, v/v/v/v). A total of 28.8 mg compound I and 57.3 mg compound II were obtained from 200 mg of flavone H-glycosides rich extract, with purities of 98.3 and 96.4%, respectively. The structures of two compounds were identified by MS and NMR. 3-O-β-D-Sophorosylkaempferol-7-O-{3-O-[2(E)-2,6-dimethyl-6-hydroxyocta-2,7-dienoyl]}-α-L-rhamnoside is compound I and compound II named hippophanone is a new compound were identified by MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of flavone glycosides from H. rhamnoides L. seed residues.

  • three new alkaloids from Hippophae rhamnoides linn subsp sinensis rousi
    ChemInform, 2015
    Co-Authors: Jian Ouyang, Wenna Zhou, Chenxu Ding, Xiaoyan Wang, Yourui Suo, Honglun Wang
    Abstract:

    The new organic amide alkaloids (I) and (II) are isolated along with a rare pyridoindole alkaloid, hippophamide (III), from the seed residue of Hippophae rhamnoides Linn.

  • Three New Alkaloids from Hippophae rhamnoides LINN. subsp sinensis ROUSI
    Helvetica Chimica Acta, 2015
    Co-Authors: Jian Ouyang, Wenna Zhou, Chenxu Ding, Gang Li, Xiaoyan Wang, Honglun Wang
    Abstract:

    Two novel organic amide alkaloids, 4-[(E)-p-coumaroylamino]butan-1-ol (1) and 4-[(Z)-p-coumaroylamino]butan-1-ol (2), together with a rare pyridoindole alkaloid, hippophamide (3), were isolated from the seed residue of Hippophae rhamnoides LINN. subsp. sinensis ROUSI. Their structures were determined by spectroscopic means. The results show that compounds 1 and 2 are (E/Z)-isomers, compound 3, a pyridoindole alkaloid concerted with gamma-lactam ring.

Yourui Suo - One of the best experts on this subject based on the ideXlab platform.

  • molecular sex identification in dioecious Hippophae rhamnoides l via rapd and scar markers
    Molecules, 2018
    Co-Authors: Wu Zhou, Honglun Wang, Yuwei Wang, Gong Zhang, Guangxiang Luan, Shasha Chen, Jing Meng, Yourui Suo
    Abstract:

    The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.

  • Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers
    MDPI AG, 2018
    Co-Authors: Wu Zhou, Honglun Wang, Yuwei Wang, Gong Zhang, Guangxiang Luan, Shasha Chen, Jing Meng, Yourui Suo
    Abstract:

    The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications

  • Isolation and structure determination of a new flavone glycoside from seed residues of seabuckthorn (Hippophae rhamnoides L.).
    Natural product research, 2017
    Co-Authors: Wenna Zhou, Zhenzhen Yuan, Ouyang Jian, Yourui Suo, Honglun Wang
    Abstract:

    In this study, a valid method was established for the isolation and purification of flavone glycosides from Hippophae rhamnoides L. seed residues using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-methanol-n-butyl alcohol-water (9:1:0.5:9, v/v/v/v). A total of 28.8 mg compound I and 57.3 mg compound II were obtained from 200 mg of flavone H-glycosides rich extract, with purities of 98.3 and 96.4%, respectively. The structures of two compounds were identified by MS and NMR. 3-O-β-D-Sophorosylkaempferol-7-O-{3-O-[2(E)-2,6-dimethyl-6-hydroxyocta-2,7-dienoyl]}-α-L-rhamnoside is compound I and compound II named hippophanone is a new compound were identified by MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of flavone glycosides from H. rhamnoides L. seed residues.

  • three new alkaloids from Hippophae rhamnoides linn subsp sinensis rousi
    ChemInform, 2015
    Co-Authors: Jian Ouyang, Wenna Zhou, Chenxu Ding, Xiaoyan Wang, Yourui Suo, Honglun Wang
    Abstract:

    The new organic amide alkaloids (I) and (II) are isolated along with a rare pyridoindole alkaloid, hippophamide (III), from the seed residue of Hippophae rhamnoides Linn.

Baoru Yang - One of the best experts on this subject based on the ideXlab platform.

  • inositols and methylinositols in sea buckthorn Hippophae rhamnoides berries
    Journal of Chromatography B, 2009
    Co-Authors: Heikki Kallio, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Marika Lassila, Eila Jarvenpaa
    Abstract:

    Abstract Sea buckthorn (Hippophae rhamnoides L.) berries, especially of ssp. sinensis, contain significant quantities of an unknown, water-soluble compound, evidently a cyclitol derivative. The compound was isolated by HPLC and analyzed by GC–MS [trimethylsilyl (TMS) derivative, selected ion monitoring (SIM) and total ion chromatogram (TIC) analyses], by 1H and 13C NMR and by optical activity measurements. The results together with analyses of reference compound verified the unambiguous structure (−)-2-O-methyl- l -chiro-inositol ( l -quebrachitol). In addition, chiro-inositol and myo-inositol existing in trace amounts were identified based on reference compounds, chromatographic data and mass spectra of the TMS derivatives. Methyl-myo-inositol was tentatively identified based on chromatography and mass spectrometry. Inositols and methyl inositols are bioactive compounds essential for regulating physiological processes of plants and humans. To our knowledge, this is the first report on the presence of chiro-inositol and myo-inositol in sea buckthorn and l -quebrachitol in edible berries. The identification of the inositols and l -quebrachitol in sea buckthorn may bring new insights into the sensory properties and also mechanisms behind the health effects of the berry.

  • fast analysis of sugars fruit acids and vitamin c in sea buckthorn Hippophae rhamnoides l varieties
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Katja Tiitinen, Baoru Yang, Gudmundur G Haraldsson, Sigridur Jonsdottir, Heikki Kallio
    Abstract:

    A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography...

  • effects of sea buckthorn Hippophae rhamnoides l seed and pulp oils on experimental models of gastric ulcer in rats
    Fitoterapia, 2002
    Co-Authors: Jianfeng Xing, Baoru Yang, Yaling Dong, Bingwen Wang, Junxian Wang, Heikki Kallio
    Abstract:

    Oils from sea buckthorn (Hippophae rhamnoides L.) seeds and berries have traditionally been used in the treatment of disorders of skin and mucosa in China. Compared with the negative control, oral administration of CO(2)-extracted seed and pulp oils, 7.0 ml x kg(-1) x day(-1) significantly reduced ulcer formation in water-immersion (P < 0.05) and reserpine-induced (P < 0.01) models in rats. In addition, administration of the two oils, 3.5 ml x kg(-1) x day(-1), significantly reduced the index of pylorus ligation-induced gastric ulcer (P < 0.05) and sped up the healing process of acetic acid-induced gastric ulcer (P < 0.01). The results suggested that the CO(2)-extracted sea buckthorn seed and pulp oils have both preventive and curative effects against experimental gastric ulcers in rats.

  • effects of different origins and harvesting time on vitamin c tocopherols and tocotrienols in sea buckthorn Hippophae rhamnoides berries
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Heikki Kallio, Baoru Yang, Pekka Peippo
    Abstract:

    Vitamin C, tocopherols, and tocotrienols in berries of wild and cultivated sea buckthorn (Hippophae rhamnoides L.) of different origins and harvesting dates were determined with HPLC. Wild berries of subsp. sinensis, native to China, contained 5−10 times more vitamin C in the juice fraction than the berries of subsp. rhamnoides from Europe and subsp. mongolica from Russia (4−13 vs 0.02−2 g/L juice). Genetic background and berry-harvesting date were two primary factors determining the vitamin C content in the berries. Crossing different subspecies influenced the vitamin C content to some extent. For bushes cultivated in southwest Finland, the best berry-harvesting date for high vitamin C content was the end of August. The seeds of subsp. sinensis contained less tocopherols and tocotrienols (average 130 mg/kg) compared with seeds of subsp. rhamnoides (average 290 mg/kg) and mongolica (average 250 mg/kg). The fruit flesh of sinensis berries had contents of tocopherols and tocotrienols 2−3 times higher than t...

  • phytosterols in sea buckthorn Hippophae rhamnoides l berries identification and effects of different origins and harvesting times
    Journal of Agricultural and Food Chemistry, 2001
    Co-Authors: Baoru Yang, Riina M Karlsson, Pentti Oksman, Heikki Kallio
    Abstract:

    Sterols in seeds, pulp/peel fractions, and whole berries of sea buckthorn (Hippophae rhamnoides L.) samples belonging to two major subspecies (sinensis and rhamnoides) from Finland and China were analyzed as TMS derivatives by gas chromatography-mass spectrometry after saponification of the oils. The total sterol contents in the seeds, the fresh pulp/peel, and the whole berries were 1200-1800, 240-400, and 340-520 mg/kg, respectively. The corresponding values in the extracted oils were 12-23, 10-29, and 13-33 g/kg. Sitosterol constituted 57-76 and 61-83%, respectively, of the seed and pulp/peel sterols. The sterol content and composition showed little variation between subspecies and collection sites. Different harvesting dates showed significant effects on the levels of some sterols both in the seeds and in the pulp/peel. The sterol profiles obtained are useful for characterizing sea buckthorn and detecting adulterations of the valuable oils. The information provided by the present investigation is also important for further chemical investigation of sea buckthorn sterols and industrial utilization of the berries as a raw material of functional foods.