The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform

Guosong Hong - One of the best experts on this subject based on the ideXlab platform.

  • traumatic brain injury imaging in the second near Infrared Window with a molecular fluorophore
    Advanced Materials, 2016
    Co-Authors: Shuo Diao, Huasen Wang, Guosong Hong, Alexander L Antaris, Lulin Li, Andy Nguyen, Joy Wang, Xiaodong Zhang, Joseph M Castellano
    Abstract:

    Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. A bright, renal-excreted, and biocompatible near-Infrared II fluorophore for in vivo imaging of TBI is designed. A transient hypoperfusion in the injured cerebral region, followed by fluorophore leakage, is observed. NIR-II fluorophores can provide noninvasive assessment of TBI.© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Language: en

  • In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes
    Methods of Molecular Biology, 2016
    Co-Authors: Guosong Hong
    Abstract:

    : In vivo fluorescence imaging in the second near-Infrared Window (NIR-II Window, 1000-1700 nm) is a powerful imaging technique that emerged in recent years. This imaging tool allows for noninvasive, deep-tissue visualization and interrogation of anatomical features and functions with improved imaging resolution and contrast at greater tissue penetration depths than traditional fluorescence imaging. Here, we present the detailed protocol for conducting NIR-II fluorescence imaging in live animals, including the procedures for preparation of biocompatible and NIR-II fluorescent carbon nanotube solution, live animal administration and NIR-II fluorescence image acquisition.

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-Infrared regions (400-900 nm) is an alternative but currently requires craniotomy, cranial Windows and skull thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 micrometre near-Infrared Window. Reduced photon scattering in this spectral region allows fluorescence imaging reaching a depth of >2 mm in mouse brain with sub-10 micrometre resolution. An imaging rate of ~5.3 frames/s allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    Near-Infrared photoluminescence from carbon nanotubes makes it possible to optically image the vasculature in the brain directly through the skull.

  • ultrafast fluorescence imaging in vivo with conjugated polymer fluorophores in the second near Infrared Window
    Nature Communications, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Xiaodong Zhang, Yingping Zou, Kai Cheng, Changxi Che, O Liu, Ju Yua, O Zhang
    Abstract:

    In vivo fluorescence imaging in the second near-Infrared Window (1.0-1.7 μm) can afford deep tissue penetration and high spatial resolution, owing to the reduced scattering of long-wavelength photons. Here we synthesize a series of low-bandgap donor/acceptor copolymers with tunable emission wavelengths of 1,050-1,350 nm in this Window. Non-covalent functionalization with phospholipid-polyethylene glycol results in water-soluble and biocompatible polymeric nanoparticles, allowing for live cell molecular imaging at >1,000 nm with polymer fluorophores for the first time. Importantly, the high quantum yield of the polymer allows for in vivo, deep-tissue and ultrafast imaging of mouse arterial blood flow with an unprecedented frame rate of >25 frames per second. The high time-resolution results in spatially and time resolved imaging of the blood flow pattern in cardiogram waveform over a single cardiac cycle (~200 ms) of a mouse, which has not been observed with fluorescence imaging in this Window before.

Alexander L Antaris - One of the best experts on this subject based on the ideXlab platform.

  • live imaging of follicle stimulating hormone receptors in gonads and bones using near Infrared ii fluorophore
    Chemical Science, 2017
    Co-Authors: Yi Feng, Shuo Diao, Alexander L Antaris, Hao Chen, Yuling Xiao, Xiaowei Lu, Linlin Jiang, Kuai Yu, Yan Wang
    Abstract:

    In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-Infrared Window (NIR-II, 1000–1700 nm) region affords reduced photon scattering in tissues accompanied by lower autofluorescence, leading to higher spatial resolution at up to centimeter tissue penetration depths. Here, we report the conjugation of a small molecular NIR-II fluorophore CH1055 to a follicle stimulating hormone (FSH-CH) for imaging ovaries and testes in live mice. After exposure to FSH-CH, specific NIR-II signals were found in cultured ovarian granulosa cells containing FSH receptors. Injection of FSH-CH allowed live imaging of ovarian follicles and testicular seminiferous tubules in female and male adult mice, respectively. Using prepubertal mice, NIR-II signals were detected in ovaries containing only preantral follicles. Resolving earlier controversies regarding the expression of FSH receptors in cultured osteoclasts, we detected for the first time specific FSH receptor signals in bones in vivo. The present imaging of FSH receptors in live animals using a ligand-conjugated NIR-II fluorophore with low cell toxicity and rapid clearance allows the development of non-invasive molecular imaging of diverse hormonal target cells in vivo.

  • rational design of molecular fluorophores for biological imaging in the nir ii Window
    Advanced Materials, 2017
    Co-Authors: Huasen Wang, Alexander L Antaris, Qinglai Yang, Yeteng Zhong, Bin Zhou, J Wang, Xiao Zhang, Jingyi Yang, Xiaodong Zhang
    Abstract:

    : A new design for second near-Infrared Window (NIR-II) molecular fluorophores based on a shielding unit-donor-acceptor-donor-shielding unit (S-D-A-D-S) structure is reported. With 3,4-ethylenedioxy thiophene as the donor and fluorene as the shielding unit, the best performance fluorophores IR-FE and IR-FEP exhibit an emission quantum yield of 31% in toluene and 2.0% in water, respectively, representing the brightest organic dyes in NIR-II region reported so far.

  • molecular imaging of biological systems with a clickable dye in the broad 800 to 1 700 nm near Infrared Window
    Proceedings of the National Academy of Sciences of the United States of America, 2017
    Co-Authors: Shuo Diao, Huasen Wang, Alexander L Antaris, Qinglai Yang, Joy Wang, Bo Zhang, Wei Huang, Xiaoyang Li, Yeteng Zhong
    Abstract:

    Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-Infrared (NIR-I; ∼700–900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000–1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II Windows (800–1,700 nm).

  • traumatic brain injury imaging in the second near Infrared Window with a molecular fluorophore
    Advanced Materials, 2016
    Co-Authors: Shuo Diao, Huasen Wang, Guosong Hong, Alexander L Antaris, Lulin Li, Andy Nguyen, Joy Wang, Xiaodong Zhang, Joseph M Castellano
    Abstract:

    Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. A bright, renal-excreted, and biocompatible near-Infrared II fluorophore for in vivo imaging of TBI is designed. A transient hypoperfusion in the injured cerebral region, followed by fluorophore leakage, is observed. NIR-II fluorophores can provide noninvasive assessment of TBI.© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Language: en

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-Infrared regions (400-900 nm) is an alternative but currently requires craniotomy, cranial Windows and skull thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 micrometre near-Infrared Window. Reduced photon scattering in this spectral region allows fluorescence imaging reaching a depth of >2 mm in mouse brain with sub-10 micrometre resolution. An imaging rate of ~5.3 frames/s allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.

Shuo Diao - One of the best experts on this subject based on the ideXlab platform.

  • live imaging of follicle stimulating hormone receptors in gonads and bones using near Infrared ii fluorophore
    Chemical Science, 2017
    Co-Authors: Yi Feng, Shuo Diao, Alexander L Antaris, Hao Chen, Yuling Xiao, Xiaowei Lu, Linlin Jiang, Kuai Yu, Yan Wang
    Abstract:

    In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-Infrared Window (NIR-II, 1000–1700 nm) region affords reduced photon scattering in tissues accompanied by lower autofluorescence, leading to higher spatial resolution at up to centimeter tissue penetration depths. Here, we report the conjugation of a small molecular NIR-II fluorophore CH1055 to a follicle stimulating hormone (FSH-CH) for imaging ovaries and testes in live mice. After exposure to FSH-CH, specific NIR-II signals were found in cultured ovarian granulosa cells containing FSH receptors. Injection of FSH-CH allowed live imaging of ovarian follicles and testicular seminiferous tubules in female and male adult mice, respectively. Using prepubertal mice, NIR-II signals were detected in ovaries containing only preantral follicles. Resolving earlier controversies regarding the expression of FSH receptors in cultured osteoclasts, we detected for the first time specific FSH receptor signals in bones in vivo. The present imaging of FSH receptors in live animals using a ligand-conjugated NIR-II fluorophore with low cell toxicity and rapid clearance allows the development of non-invasive molecular imaging of diverse hormonal target cells in vivo.

  • molecular imaging of biological systems with a clickable dye in the broad 800 to 1 700 nm near Infrared Window
    Proceedings of the National Academy of Sciences of the United States of America, 2017
    Co-Authors: Shuo Diao, Huasen Wang, Alexander L Antaris, Qinglai Yang, Joy Wang, Bo Zhang, Wei Huang, Xiaoyang Li, Yeteng Zhong
    Abstract:

    Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-Infrared (NIR-I; ∼700–900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000–1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II Windows (800–1,700 nm).

  • traumatic brain injury imaging in the second near Infrared Window with a molecular fluorophore
    Advanced Materials, 2016
    Co-Authors: Shuo Diao, Huasen Wang, Guosong Hong, Alexander L Antaris, Lulin Li, Andy Nguyen, Joy Wang, Xiaodong Zhang, Joseph M Castellano
    Abstract:

    Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. A bright, renal-excreted, and biocompatible near-Infrared II fluorophore for in vivo imaging of TBI is designed. A transient hypoperfusion in the injured cerebral region, followed by fluorophore leakage, is observed. NIR-II fluorophores can provide noninvasive assessment of TBI.© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Language: en

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-Infrared regions (400-900 nm) is an alternative but currently requires craniotomy, cranial Windows and skull thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 micrometre near-Infrared Window. Reduced photon scattering in this spectral region allows fluorescence imaging reaching a depth of >2 mm in mouse brain with sub-10 micrometre resolution. An imaging rate of ~5.3 frames/s allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    Near-Infrared photoluminescence from carbon nanotubes makes it possible to optically image the vasculature in the brain directly through the skull.

Katrin Andreasson - One of the best experts on this subject based on the ideXlab platform.

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-Infrared regions (400-900 nm) is an alternative but currently requires craniotomy, cranial Windows and skull thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 micrometre near-Infrared Window. Reduced photon scattering in this spectral region allows fluorescence imaging reaching a depth of >2 mm in mouse brain with sub-10 micrometre resolution. An imaging rate of ~5.3 frames/s allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.

  • through skull fluorescence imaging of the brain in a new near Infrared Window
    Nature Photonics, 2014
    Co-Authors: Guosong Hong, Shuo Diao, Alexander L Antaris, Junlei Chang, Changxin Chen, Bo Zhang, Su Zhao, Dmitriy N Atochin, Paul L Huang, Katrin Andreasson
    Abstract:

    Near-Infrared photoluminescence from carbon nanotubes makes it possible to optically image the vasculature in the brain directly through the skull.

Qiangbin Wang - One of the best experts on this subject based on the ideXlab platform.

  • controlled synthesis of ag2te ag2s core shell quantum dots with enhanced and tunable fluorescence in the second near Infrared Window
    Small, 2020
    Co-Authors: Yejun Zhang, Hongchao Yang, Xinyi An, Zan Wang, Xiaohu Yang, Mengxuan Yu, Rong Zhang, Qiangbin Wang
    Abstract:

    : Fluorescence in the second near-Infrared Window (NIR-II, 900-1700 nm) has drawn great interest for bioimaging, owing to its high tissue penetration depth and high spatiotemporal resolution. NIR-II fluorophores with high photoluminescence quantum yield (PLQY) and stability along with high biocompatibility are urgently pursued. In this work, a Ag-rich Ag2 Te quantum dots (QDs) surface with sulfur source is successfully engineered to prepare a larger bandgap of Ag2 S shell to passivate the Ag2 Te core via a facile colloidal route, which greatly enhances the PLQY of Ag2 Te QDs and significantly improves the stability of Ag2 Te QDs. This strategy works well with different sized core Ag2 Te QDs so that the NIR-II PL can be tuned in a wide range. In vivo imaging using the as-prepared Ag2 Te@Ag2 S QDs presents much higher spatial resolution images of organs and vascular structures as compared with the same dose of Ag2 Te nanoprobes administrated, suggesting the success of the core-shell synthetic strategy and the potential biomedical applications of core-shell NIR-II nanoprobes.

  • Programmable Chemotherapy and Immunotherapy against Breast Cancer Guided by Multiplexed Fluorescence Imaging in the Second Near-Infrared Window.
    Advanced Materials, 2018
    Co-Authors: Chunyan Li, Guangcun Chen, Yejun Zhang, Haozhi Wang, Mao Wang, Qiangbin Wang
    Abstract:

    : Combined chemotherapy and immunotherapy have demonstrated great potential in cancer treatment. However, it is difficult to provide clear information of the pharmacokinetics and pharmacodynamics of chemodrugs and transplanted immune cells in vivo by traditional approaches, resulting in inadequate therapy. Here, a multiplexed intravital imaging strategy by using fluorescence in the second near-Infrared Window (NIR-II) is first developed to visualize the two events of chemotherapy and immunotherapy in vivo, so that a combinational administration is programed to improve the therapeutical effects against a mouse model of human breast cancer. In detail, Ag2 Se quantum dots (QDs) (λEm = 1350 nm) loaded with stromal-cell-derived factor-1α (SDF-1α) and chemodrug doxorubicin (DOX) are first administrated to deliver the SDF-1α and DOX to the tumor site. After their arrival, monitored by Ag2 Se QD fluorescence, natural killer (NK)-92 cells labeled with Ag2 S QDs (λEm = 1050 nm) are intravenously injected so that the cells are recruited to the tumor by the chemotaxis of SDF-1α, which is visualized by Ag2 S QD fluorescence. Such an imaging approach allows simultaneous evaluation of the behaviors of individual injections in vivo, and facilitates optimized administration regimens, resulting in enhanced tumor inhibition.

  • surface plasmon resonance enhanced light absorption and photothermal therapy in the second near Infrared Window
    Journal of the American Chemical Society, 2014
    Co-Authors: Xianguang Ding, Chunyan Li, Qiangbin Wang, Chihao Liow, Mengxin Zhang, Renjun Huang, He Shen, Zhijun Zhang, Yonggang Li, Shuzhou Li
    Abstract:

    Enhanced near-field at noble metal nanoparticle surfaces due to localized surface plasmon resonance (LSPR) has been researched in fields ranging from biomedical to photoelectrical applications. However, it is rarely explored on nonmetallic nanomaterials discovered in recent years, which can also support LSPR by doping-induced free charge carriers, let alone the investigation of an intricate system involving both. Here we construct a dual plasmonic hybrid nanosystem Au–Cu9S5 with well controlled interfaces to study the coupling effect of LSPR originating from the collective electron and hole oscillations. Cu9S5 LSPR is enhanced by 50% in the presence of Au, and the simulation results confirm the coupling effect and the enhanced local field as well as the optical power absorption on Cu9S5 surface. This enhanced optical absorption cross section, high photothermal transduction efficiency (37%), large light penetration depth at 1064 nm, excellent X-ray attenuation ability, and low cytotoxicity enable Au–Cu9S5 ...

  • Facile Synthesis of Highly Photoluminescent Ag2Se Quantum Dots as a New Fluorescent Probe in the Second Near-Infrared Window for in Vivo Imaging
    Chemistry of Materials, 2013
    Co-Authors: Bohua Dong, Chunyan Li, Guangcun Chen, Yejun Zhang, Yan Zhang, Manjiao Deng, Qiangbin Wang
    Abstract:

    A facile solvothermal method is reported to synthesize highly photoluminescent Ag2Se quantum dots (QDs) with emission at 1300 nm in the second near-Infrared Window. After surface modification of C18-PMH-PEG, the Ag2Se QDs possess bright photoluminescence, good water-solubility, high colloidal stability and photostability, as well as decent biocompatibility, which are further successfully performed in in vivo deep imaging of organs and vascular structures with high spatial resolution. This new NIR-II fluorescent nanoprobe with small sizes, ideal optical properties, and decent biocompatibility opens up exciting opportunities for future biomedical applications.

  • ag2s quantum dot a bright and biocompatible fluorescent nanoprobe in the second near Infrared Window
    ACS Nano, 2012
    Co-Authors: Yan Zhang, Guosong Hong, Guangcun Chen, Yejun Zhang, Feng Li, Qiangbin Wang
    Abstract:

    Ag2S quantum dots (QDs) emitting in the second near-Infrared region (NIR-II, 1.0–1.4 μm) are demonstrated as a promising fluorescent probe with both bright photoluminescence and high biocompatibility for the first time. Highly selective in vitro targeting and imaging of different cell lines are achieved using biocompatible NIR-II Ag2S QDs with different targeting ligands. The cytotoxicity study illustrates the Ag2S QDs with negligible effects in altering cell proliferation, triggering apoptosis and necrosis, generating reactive oxygen species, and causing DNA damage. Our results have opened up the possibilities of using these biocompatible Ag2S QDs for in vivo anatomical imaging and early stage tumor diagnosis with deep tissue penetration, high sensitivity, and elevated spatial and temporal resolution owing to their high emission efficiency in the unique NIR-II imaging Window.