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David Betancurancona - One of the best experts on this subject based on the ideXlab platform.
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in vitro renin angiotensin system inhibition and in vivo antihypertensive activity of peptide fractions from Lima Bean phaseolus lunatus l
Journal of the Science of Food and Agriculture, 2018Co-Authors: Norma Ciausolis, Juan Jose Acevedofernandez, David BetancuranconaAbstract:BACKGROUND The renin-angiotensin system is key in the physiopathology of arterial hypertension because it converts angiotensin I, via angiotensin I-converting enzyme (ACE), into angiotensin II. In vitro analyses were done of the ACE-inhibitory and renin-inhibitory activities of peptide fractions isolated by enzymatic hydrolysis of Lima Bean (Phaseolus lunatus) protein. Antihypertensive activity was confirmed in vivo using a rat model. RESULTS Lima Bean protein was hydrolyzed with one of two sequential enzymatic systems (pepsin-pancreatin or Alcalase®-Flavourzyme®). Ultrafiltration of the hydrolysates produced fractions of different molecular weights. The >3 kDa fraction of the pepsin-pancreatin hydrolysate had the highest ACE-inhibitory activity (60.15%, IC50: 172.62 μg ml-1) while the >3 KDa fraction of the Alcalase®-Flavourzyme® hydrolysate had the highest in vitro renin-inhibitory activity. A weak correlation (r=0.44) was found between ACE-inhibitory and renin-inhibitory activities. When tested in vivo, the latter fraction lowered systolic blood pressure by 64% and diastolic blood pressure by 51%. CONCLUSION Peptide fractions from Lima Bean Phaseolus lunatus protein hydrolysates exhibit both in vitro and in vivo antihypertensive activity. Bioactive peptides from Lima Bean have potential applications as ingredients in functional foods.
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in vitro renin angiotensin system inhibition and in vivo antihypertensive activity of peptide fractions from Lima Bean phaseolus lunatus l
Journal of the Science of Food and Agriculture, 2018Co-Authors: Norma Ciausolis, Juan Jose Acevedofernandez, David BetancuranconaAbstract:BACKGROUND The renin-angiotensin system is key in the physiopathology of arterial hypertension because it converts angiotensin I, via angiotensin I-converting enzyme (ACE), into angiotensin II. In vitro analyses were done of the ACE-inhibitory and renin-inhibitory activities of peptide fractions isolated by enzymatic hydrolysis of Lima Bean (Phaseolus lunatus) protein. Antihypertensive activity was confirmed in vivo using a rat model. RESULTS Lima Bean protein was hydrolyzed with one of two sequential enzymatic systems (pepsin-pancreatin or Alcalase®-Flavourzyme®). Ultrafiltration of the hydrolysates produced fractions of different molecular weights. The >3 kDa fraction of the pepsin-pancreatin hydrolysate had the highest ACE-inhibitory activity (60.15%, IC50: 172.62 μg ml-1) while the >3 KDa fraction of the Alcalase®-Flavourzyme® hydrolysate had the highest in vitro renin-inhibitory activity. A weak correlation (r=0.44) was found between ACE-inhibitory and renin-inhibitory activities. When tested in vivo, the latter fraction lowered systolic blood pressure by 64% and diastolic blood pressure by 51%. CONCLUSION Peptide fractions from Lima Bean Phaseolus lunatus protein hydrolysates exhibit both in vitro and in vivo antihypertensive activity. Bioactive peptides from Lima Bean have potential applications as ingredients in functional foods.
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effect of octenylsuccinylation on functional properties of Lima Bean phaseolus lunatus starch
Journal of Food Process Engineering, 2009Co-Authors: Maira Rubi Seguracampos, Luis Chelguerrero, David BetancuranconaAbstract:ABSTRACT The functional properties of Lima Bean (Phaseolus lunatus) starch modified with octenyl succinic anhydride (OSA) were evaluated and compared with native starch. The OSA starch was produced from a starch aqueous suspension at 40% (w/w) with 3% OSA at pH 7 for 30 min. The modified starch had 0.51% succinyl groups and a 0.008 degree of substitution. Succinylation increased emulsifying capacity from 0.47 mL oil/mL sample in the native starch to 0.53 mL oil/mL sample in the OSA starch. Starch gel clarity (transmittance percentage) increased from 23.8 to 37.3%. Viscosity increased from 700 to 1,000 BU. Gel firmness decreased from 0.3401 kgf for gel rupture in the native starch to 0.0314 kgf for rupture in the OSA starch. Gelatinization temperature decreased from 75.3 to 64.6C and gel enthalpy decreased from 10.7 to 9.7 J/g. Succinylation caused not significant changes in solubility, swelling power and water absorption capacity. OSA starch stability was lower under refrigeration conditions. PRACTICAL APPLICATIONS The incorporation of octenylsuccinate groups into Lima Bean starch produce a derivative with more versatility as a food additive providing new and improved functional properties. The octenylsuccinylation allows the use of the Lima Bean starch in products such as beverages, salad dressings and soups where the use of this starch is limited because lack of diversity in their functional properties necessaries in the alimentary industry. Therefore, the improved properties of the Phaseolus lunatus octenyl succinic anhydride starch make it potentially useful in processes requiring a thickening agent that must gel at lower temperatures; it can also serve to reduce energy consumption during cooking processes or like thickening or emulsifying agent or make it potentially useful as an additive in jellies and candies to provide brightness. However, the election of this derived as additive in food, should take into account the functional properties that it presents, the ingredients and the process conditions.
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physicochemical characterization of Lima Bean phaseolus lunatus and jack Bean canavalia ensiformis fibrous residues
Food Chemistry, 2004Co-Authors: David Betancurancona, Gwendolyne Perazamercado, Yolanda Moguelordonez, David Betancurancona, Sonia FuertesblancoAbstract:Some physicochemical, physiological and functional characteristics of Jack Bean (Canavalia ensiformis) and Lima Bean (Phaseolus lunatus) fibrous residues, obtained as a by-product during the protein and starch extraction process were evaluated. The C. ensiformis residues had higher crude fibre (22.6%), total dietary fibre (55.8%) and insoluble dietary fibre (52.4%) contents than the P. lunatus (crude fibre, 12.8%; total dietary fibre, 29.4%; insoluble dietary fibre, 28.6%). Water-holding capacities in both legume fibrous residues were higher (C. ensiformis, 39.5%; P. lunatus, 26.5%) than their oil-holding capacities (23% and 18%, respectively). The P. lunatus residue had lower emulsifying activity (8.6%) than the C. ensiformis residues (49.3%) but higher emulsifying stability (P. lunatus, 100% versus C. ensiformis, 28.25%). Neither fibrous residue was capable of bonding calcium ions, but both did bind iron ions (C. ensiformis, 31.1%; P. lunatus, 28.4%). Similar antioxidant activity values were obtained for the Jack Bean (39.4%) and Lima Bean (35.6%) residues.
Jean-pierre Baudoin - One of the best experts on this subject based on the ideXlab platform.
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Genetic structure of Lima Bean (Phaseolus lunatus L.) landraces grown in the Mayan area
Genetic Resources and Crop Evolution, 2018Co-Authors: Luciana Camacho-pérez, Jean-pierre Baudoin, Jaime Martínez-castillo, Javier O. Mijangos-cortés, Miriam M. Ferrer-ortega, Rubén H. Andueza-nohAbstract:Lima Bean ( Phaseolus lunatus L.) is an important crop in the Mayan culture. The Mayan area, considered as a main center of Mesoamerican diversity, has been divided into two subareas: the Mayan lowlands and the Mayan highlands. The Yucatan Peninsula is part of the Mayan lowlands and holds the highest number of Lima Bean landraces of Mexico, but Lima Beans are in high risk of genetic erosion due to intensification of the traditional Mayan agriculture. However, information on genetic diversity of Lima Beans of the Mayan highlands is lacking. By using 46 landraces collected in the Mayan area (23 from each subarea) and 73 ISSR loci (inter-simple sequence repeats), we analyzed the structure, diversity and genetic relationships of Lima Beans of this part of Mesoamerica. High levels of diversity ( H _BAY = 0.45) and genetic structure ( F _ST = 0.66) were found for the whole Mayan area. Genetic diversity in the Mayan lowlands was apparently higher than the Mayan highlands ( H _BAY = 0.44 and 0.36, respectively); but differences were not statistically significant. Genetic structure between the subareas was high (AMOVA = 30% of total variation), most landraces grouping according to their geographic origin. This study shows the importance of the Mayan culture in the diversification and conservation of Lima Beans. The results provide important information that should be considered when implementing strategies to collect Lima Bean landraces and planning in situ and ex situ programs to conserve these landraces in the Mayan region.
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Lima Bean: Phaseolus lunatus L.
Genetic Improvement of Vegetable Crops, 2012Co-Authors: Jean-pierre BaudoinAbstract:Publisher Summary The food legume Phaseolus lunatus L.—commonly called Lima Bean—belongs to the genus Phaseolus, the subtribe Phaseolinae, the tribe Phaseoleae, the family Papilionaceae or Fabaceae, and the order of Leguminosales or Fabales. Phaseolus lunatus has a neotropical origin with at least two major centers of diversity as suggested by the electrophoretic analysis of crude seed protein: (1) the Central American center (mainly Mexico and Guatemala) for the small-seeded forms and (2) the Andean center (mainly Peru and Ecuador) for the large-seeded forms. Two varieties have been botanically identified within the species: (1) var. Silvester (the wild forms) and (2) var. lunatus (the cultivated forms). The latter is subdivided into three major cultigroups: (1) cv-gr. Sieva with medium-sized, flat seeds, (2) cv-gr. Potato with small, globular seeds, and (3) cv-gr. Big Lima with large, flat seeds. This classification, however, is far from reflecting the whole range of seed diversity among the cultivated materials due to free gene flow occurring between the three cultigroups. The Lima Bean is a herbaceous species, including annual determinate bush types and indeterminate climbing types. This chapter discusses the cytology, genetics, germplasm resources, reproductive biology, breeding objectives, and breeding methods of Lima Bean.
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genetic diversity in the Lima Bean phaseolus lunatus l as revealed by chloroplast dna cpdna variations
Genetic Resources and Crop Evolution, 2001Co-Authors: B Fofana, Patrick Du Jardin, Jean-pierre BaudoinAbstract:Genetic diversity in the Lima Bean (P. lunatus L.) was assessed bymeans of two chloroplast DNA probes. Based on data obtained from 152accessions including wild forms and landraces, the two separateMesoamerican and Andean groups were confirmed and a transition poolof genetic diversity was observed in the two botanical formsconsistent with their distribution range. Three primary centres ofgenetic diversity and one secondary diversification spot are proposedfor the wild Lima Bean whereas only two domestication sites areunderlined for the landraces. The importance of human intervention inwidening the distribution range and the genetic diversity inlandraces is discussed.
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phylogenetic study on wild allies of Lima Bean phaseolus lunatus fabaceae and implications on its origin
Plant Systematics and Evolution, 1999Co-Authors: Alain Maquet, Xavier Vekemans, Jean-pierre BaudoinAbstract:An investigation was made of the phylogenetic relationships among wild accessions of Lima Bean (Phaseolus lunatus) and wild allies of Mesoamerican and Andean origins, using electrophoresis of seed storage proteins and isozymes. Mesoamerican wild species are phylogenetically more distant fromP. lunatus than Andean species, and apparently belong to the tertiary gene pool of Lima Bean. The Andean wild species, which are investigated for the first time, reveal a high similarity to the Lima Bean, and particularly with its Mesoamerican gene pool. These Andean species probably constitute a secondary gene pool of Lima Bean, and are thus of considerable interest in the context of genetic improvement of the crop. Based on these observations, an Andean origin is suggested for the Andean wild species and forP. lunatus. These results point out the importance of collecting and conserving AndeanPhaseolus germplasm.
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Sample size for collecting seeds in germplasm conservation: the case of the Lima Bean (Phaseolus lunatus L.)
Theoretical and Applied Genetics, 1998Co-Authors: Alain Maquet, Bernard Wathelet, Jérôme Degreef, Jean-pierre BaudoinAbstract:The design of optimum sampling strategies integrating criteria of efficiency relevant to multilocus models and many target populations has been investigated with respect to the number of plants and the number of seeds per plant to be sampled for a Lima Bean (Phaseolus lunatus L.) gene pool. This study, using five populations and six polymorphic enzyme loci, shows that the number of plants rather than the number of seeds collected per plant primarily determines the success of seed sampling, suggesting that plant number plays an essential part in maintaining the allelic multiplicity of predominantly selfing species like Lima Bean. According to the results, it appears that among Lima Bean populations an efficient sampling procedure is achieved by collecting 1–4 seeds from 200 to 300 plants. These sample sizes will retain 8–10 alleles, regardless of their frequencies. When we consider polymorphism at the 5% level, it is expected that sampling 10–80 plants will collect combinations of 4–8 alleles. Based on data from genetic and demographic studies, we suggest an efficient sampling scheme for Lima Bean germplasm at both population and geographical levels.
Junji Takabayashi - One of the best experts on this subject based on the ideXlab platform.
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Characterization of the promoter sequence of chitinase gene from Lima Bean plant
Journal of Plant Interactions, 2011Co-Authors: Koichi Sugimoto, Rika Ozawa, Kenji Matsui, Junji TakabayashiAbstract:Abstract Chitinase is involved in induced defense against biotic attacks. We analyzed the promoter of the gene in Lima Bean plants and found cis-elements important for the induction under stress.
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intraspecies variation in the kanzawa spider mite differentially affects induced defensive response in Lima Bean plants
Journal of Chemical Ecology, 2006Co-Authors: Ryo Matsushima, Rika Ozawa, Masayoshi Uefune, Tetsuo Gotoh, Junji TakabayashiAbstract:The Kanzawa spider mite, Tetranychus kanzawai, is a polyphagous herbivore that feeds on various plant families, including the Leguminacae. Scars made by the mite on Lima Bean leaves (Phaseolus lunatus) were classified into two types: white and red. We obtained two strains of mites—“White” and “Red”—by selecting individual mites based on the color of the scars. Damage made by the Red strain induced the expression of genes for both basic chitinase, which was downstream of the jasmonic acid (JA) signaling pathway, and acidic chitinase, which was downstream of the salicylic acid (SA) signaling pathway. White strain mites also induced the expression of the basic chitinase gene in infested leaves but they only slightly induced the acidic chitinase gene. The Red genotype was dominant over the White for the induction of the acidic chitinase gene. The amount of endogenous salicylates in leaves increased significantly when infested by Red strain mites but did not increase when infested by White strain mites. JA and SA are known to be involved in the production of Lima Bean leaf volatiles induced by T. urticae. The blend of volatiles emitted from leaves infested by the Red strain were qualitatively different from those infested by the White strain, suggesting that the SA and JA signaling pathways are differently involved in the production of Lima Bean leaf volatiles induced by T. kanzawai of different strains.
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herbivore induced volatiles induce the emission of ethylene in neighboring Lima Bean plants
Plant Journal, 2002Co-Authors: Gen-ichiro Arimura, Rika Ozawa, Takaaki Nishioka, Wilhelm Boland, Thomas Koch, Frank Kuhnemann, Junji TakabayashiAbstract:Herbivore attacks induce leaves to emit a specific blend of volatiles. Here we show that exposure to Tetranychus urticae-induced volatiles, as well as T. urticae infestation and artificial wounding, activates the transcription of the genes involved in the biosynthesis of ethylene [S-adenosylmethionine (SAM) synthetase and 1-aminocyclopropane-1-carboxylic acid oxidase] and a gene involved in the biosynthesis of polyamines from SAM (SAM decarboxylase) in Lima Bean leaves. Moreover, exposure of leaves to any one of the seven major chemical components of T. urticae-induced volatiles also induces expression of these genes. Furthermore, we found that, when Lima Bean plants were exposed to T. urticae-induced volatiles, they emitted ethylene. Lima Bean plants infested by T. urticae and artificially wounded plants also emitted ethylene. Endogenous polyamine levels were not increased in the exposed leaves or the infested leaves, suggesting that polyamine production from SAM was only slightly promoted at the metabolic levels present in the leaves. We found that jasmonate (JA) accumulated in leaves exposed to T. urticae-induced volatiles, and that both JA and salicylate (SA) accumulated in leaves infested by T. urticae. These findings, as well as results of pharmacological analyses, suggest that, in leaves exposed to T. urticae-induced volatiles, ethylene biosynthesis might be regulated by pathways involving JA and the ethylene positive feedback loop. They also suggest that ethylene biosynthesis might be regulated by signaling pathways involving JA, SA and ethylene in T. urticae-infested leaves.
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Exogenous ACC enhances volatiles production mediated by jasmonic acid in Lima Bean leaves.
FEBS letters, 2001Co-Authors: Jun-ichiro Horiuchi, Junji Takabayashi, Gen-ichiro Arimura, Rika Ozawa, Takeshi Shimoda, Takaaki NishiokaAbstract:We report the synergistic effects of exogenous 1-aminocyclopropane-1-carboxylic acid (ACC) and jasmonic acid (JA) on production of induced volatiles by excised Lima Bean leaves. Application of ACC alone to leaves induced trace amounts of volatiles. ACC positively affected three JA-induced volatiles, (E)- and (Z)-β-ocimene, and (Z)-3-hexenyl acetate. The ethylene inhibitor, silver thiosulfate, inhibited the production of these compounds. The results suggest synergistic effects of JA and ACC on inducible volatile production by Lima Bean leaves. Furthermore, Lima Bean leaves treated with JA plus ACC became more attractive to predatory mites, Phytoseiulus persimilis, than those treated with JA alone.
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Induction of indirect defence aganist spider-mites in uninfested Lima Bean leaves
Phytochemistry, 1991Co-Authors: Junji Takabayashi, Marcel Dicke, Maarten A. PosthumusAbstract:Abstract Headspace analyses of uninfested Lima Bean ( Phaseolus lunatus ) leaves show an absence of or only trace amounts of the terpenoids ( E )-β-ocimene and ( E )-4,8-dimethyl-1,3,7-nonatriene. Upon infestation by two-spotted spider-mites ( Tetranychus urticae ), Lima Bean leaves produce ( E )-β-ocimene and ( E )-4,8-dimethyl-1,3,7-nonatriene which attract predators of the herbivore, a phenomenon known as indirect defence. When uninfested Lima Bean leaves were placed on wet cotton wool upon which leaves that were partially infested with spider-mites, were also placed, the uninfested leaf tissue emitted the terpenoids in relatively high amounts: ca 20–40% of the amounts emitted by the infested leaf tissue. The same effect was found when uninfested leaves were put on wet cotton wool on which infested leaves had previously lain. The data show that an elicitor(s) from spider-mite infested leaves induces the production of ( E )-β-ocimene and ( E )-4,8-dimethyl-1,3,7-nonatriene in uninfested Lima Bean leaves. This is the first chemical evidence for induction of indirect defence in uninfested leaves.
Alain Maquet - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic study on wild allies of Lima Bean phaseolus lunatus fabaceae and implications on its origin
Plant Systematics and Evolution, 1999Co-Authors: Alain Maquet, Xavier Vekemans, Jean-pierre BaudoinAbstract:An investigation was made of the phylogenetic relationships among wild accessions of Lima Bean (Phaseolus lunatus) and wild allies of Mesoamerican and Andean origins, using electrophoresis of seed storage proteins and isozymes. Mesoamerican wild species are phylogenetically more distant fromP. lunatus than Andean species, and apparently belong to the tertiary gene pool of Lima Bean. The Andean wild species, which are investigated for the first time, reveal a high similarity to the Lima Bean, and particularly with its Mesoamerican gene pool. These Andean species probably constitute a secondary gene pool of Lima Bean, and are thus of considerable interest in the context of genetic improvement of the crop. Based on these observations, an Andean origin is suggested for the Andean wild species and forP. lunatus. These results point out the importance of collecting and conserving AndeanPhaseolus germplasm.
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Sample size for collecting seeds in germplasm conservation: the case of the Lima Bean (Phaseolus lunatus L.)
Theoretical and Applied Genetics, 1998Co-Authors: Alain Maquet, Bernard Wathelet, Jérôme Degreef, Jean-pierre BaudoinAbstract:The design of optimum sampling strategies integrating criteria of efficiency relevant to multilocus models and many target populations has been investigated with respect to the number of plants and the number of seeds per plant to be sampled for a Lima Bean (Phaseolus lunatus L.) gene pool. This study, using five populations and six polymorphic enzyme loci, shows that the number of plants rather than the number of seeds collected per plant primarily determines the success of seed sampling, suggesting that plant number plays an essential part in maintaining the allelic multiplicity of predominantly selfing species like Lima Bean. According to the results, it appears that among Lima Bean populations an efficient sampling procedure is achieved by collecting 1–4 seeds from 200 to 300 plants. These sample sizes will retain 8–10 alleles, regardless of their frequencies. When we consider polymorphism at the 5% level, it is expected that sampling 10–80 plants will collect combinations of 4–8 alleles. Based on data from genetic and demographic studies, we suggest an efficient sampling scheme for Lima Bean germplasm at both population and geographical levels.
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Genetic structure of a Lima Bean base collection using allozyme markers
Theoretical and Applied Genetics, 1997Co-Authors: Alain Maquet, M. Delvaux, Bernard Wathelet, Jean-pierre BaudoinAbstract:Genetic diversity and structure within a Lima Bean (Phaseolus lunatus L.) base collection have been evaluated using allozyme markers. The results obtained from the analysis of wild and cultivated accessions confirm the existence of Andean and Mesoamerican gene pools characterised by specific alleles. Wild and cultivated accessions of the same gene pool are grouped. The Andean natural populations have a very limited geographic distribution between Ecuador and northern Peru. The Mesoamerican wild form extends from Mexico up to Argentina through the eastern side of the Andes. Andean and Mesoamerican cultivated accessions of pantropical distribution contribute substantially to the genetic diversity of the Lima Bean base collection. Population genetic parameters, estimated from allozymes, confirmed the predominant selfing mating system of the Lima Bean. The selfing mating system, the occurrence of small populations, and low gene flow lead to an interpopulation gene diversity (DST=0.235) higher than the intrapopulation gene diversity (HS=0.032). On the basis of the results, guidelines are given to preserve and exploit the genetic diversity of this threatened species. The results also confirm the independent domestication of the Lima Bean in at least two centres, one of which is located at medium elevation in the western valleys of Ecuador and northern Peru.
Ryo Matsushima - One of the best experts on this subject based on the ideXlab platform.
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intraspecies variation in the kanzawa spider mite differentially affects induced defensive response in Lima Bean plants
Journal of Chemical Ecology, 2006Co-Authors: Ryo Matsushima, Rika Ozawa, Masayoshi Uefune, Tetsuo Gotoh, Junji TakabayashiAbstract:The Kanzawa spider mite, Tetranychus kanzawai, is a polyphagous herbivore that feeds on various plant families, including the Leguminacae. Scars made by the mite on Lima Bean leaves (Phaseolus lunatus) were classified into two types: white and red. We obtained two strains of mites—“White” and “Red”—by selecting individual mites based on the color of the scars. Damage made by the Red strain induced the expression of genes for both basic chitinase, which was downstream of the jasmonic acid (JA) signaling pathway, and acidic chitinase, which was downstream of the salicylic acid (SA) signaling pathway. White strain mites also induced the expression of the basic chitinase gene in infested leaves but they only slightly induced the acidic chitinase gene. The Red genotype was dominant over the White for the induction of the acidic chitinase gene. The amount of endogenous salicylates in leaves increased significantly when infested by Red strain mites but did not increase when infested by White strain mites. JA and SA are known to be involved in the production of Lima Bean leaf volatiles induced by T. urticae. The blend of volatiles emitted from leaves infested by the Red strain were qualitatively different from those infested by the White strain, suggesting that the SA and JA signaling pathways are differently involved in the production of Lima Bean leaf volatiles induced by T. kanzawai of different strains.