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Maria J Amoroso - One of the best experts on this subject based on the ideXlab platform.
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An Overview on Microbial Degradation of Lindane
Microbe-Induced Degradation of Pesticides, 2016Co-Authors: Juliana Maria Saez, Maria Soledad Fuentes, Maria J Amoroso, Analia Alvarez, Claudia S BenimeliAbstract:Lindane is a cyclic, saturated and highly chlorinated pesticide with a broad spectrum, which has been used worldwide for many decades to control a variety of pests, and also in human health and veterinary. Afterward, it has been demonstrated that Lindane and its isomers may cause serious damage to health in the short and long term. Besides, Lindane is known to be persistent in the environment and tends to bioaccumulate along the food chain. Thus, Lindane residues remain in the environment for a long time and have been recently found in water, soil, sediments, plants, and animals all over the world, and even in human fluids and tissues. In this context, nowadays, scientists, working all over the world, are involved in developing Lindane remediation technologies including physical, chemical, and biological techniques. This article provides updated information on the biologic degradation of Lindane using different microorganisms such as bacteria, fungi, and algae, under both aerobic and anaerobic conditions.
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enhanced Lindane removal from soil slurry by immobilized streptomyces consortium
International Biodeterioration & Biodegradation, 2014Co-Authors: Juliana Maria Saez, Claudia S Benimeli, Maria J Amoroso, Analia AlvarezAbstract:Abstract The aim of this work was to assess Lindane removal from soil slurry by a Streptomyces consortium immobilized in cloth sachets, at different inoculum, Lindane and slurry concentrations. In concentrated slurry (soil/water ratio of 2:3), the higher Lindane removal (35.3 mg Kg −1 ) was obtained with the medium inoculum (10 7 CFU g −1 ) and the highest Lindane concentration tested, at 7 days of incubation. Although, Lindane removal was also detected in abiotic controls, probably caused by pesticide adsorption to soil particles. Thus, these parameters were selected for evaluating the pesticide removal in diluted slurry (soil/water ratio of 1:4). After 14 days of incubation, 28.7 mg Kg −1 of Lindane were removed. Also, a phytotoxicity assay demonstrated that seeds growing on diluted slurries bioremediated during 7 and 14 days, showed an improvement in biological parameters, compared to those growing on non-bioremediated slurries. Thus, bioremediated slurries would not have toxic effects on lettuce seeds.
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simultaneous bioremediation of cr vi and Lindane in soil by actinobacteria
International Biodeterioration & Biodegradation, 2014Co-Authors: Marta Alejandra Polti, Claudia S Benimeli, Maria J Amoroso, Juan Daniel AparicioAbstract:Abstract Environments co-contaminated with metals and organic compounds are difficult to remediate. Actinobacteria is an important group of microorganisms found in soils, with high metabolic versatility and potential for bioremediation. In this paper, actinobacteria were used to remediate soil co-contaminated with Cr(VI) and Lindane. Five actinobacteria, tolerant to Cr(VI) and Lindane mixture were selected: Streptomyces spp. A5, A11, M7, and MC1, and Amycolatopsis tucumanensis DSM 45259. Sterilized soil samples were inoculated with actinobacteria strains, either individually or as a consortium, and contaminated with Cr(VI) and Lindane, either immediately or after 7 days of growth, and incubated at 30 °C during 14 days. All actinobacteria were able to grow and remove both contaminants, the consortium formed by Streptomyces spp. A5, M7, MC1, and A. tucumanensis showed the highest Cr(VI) removal, while Streptomyces sp. M7 produced the maximum Lindane removal. In non-sterile soil samples, Streptomyces sp. M7 and the consortium removed more than 40% of the Lindane, while Streptomyces sp. M7 demonstrated the greatest Cr(VI) removal. The most appropriate strategy for bioremediation of Cr(VI) and Lindane co-contaminated soils would be the inoculation with Streptomyces sp. M7.
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maize plants zea mays root exudates enhance Lindane removal by native streptomyces strains
International Biodeterioration & Biodegradation, 2012Co-Authors: Analia Alvarez, Claudia S Benimeli, Maria J Amoroso, M L YanezAbstract:The organochlorine pesticide (OP) Lindane was removed from minimal medium (MM) by two Streptomyces native strains, while growing on maize root exudates (REs) as a primary carbon and energy source. REs supported 55 and 35% of Lindane removal by Streptomyces sp. strains A5 and M7, respectively, corroborating the hypothesis that co-metabolism may be a plant/microbe interaction important to bioremediation. In addition, residual Lindane concentration was more than half of the amount in MM supplemented with glucose compared to MM supplemented with REs, suggesting that exudates could be more appropriate carbon source to support aerobic dehalogenation of the pesticide. Lindane-degrading activity was detected in REs, which could explain 42% of Lindane removal in REs-Lindane assay, without microorganisms. Because Streptomyces sp. A5 showed maximum biomass and the highest pesticide removal in REs-Lindane assay, it was found to be the most promising strain regarding their future application. These results showed that phytostimulation of OP-degrading actinobacteria by maize REs are therefore likely to be a successful strategy for the remediation of Lindane-contaminated environments.
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Lindane biodegradation by defined consortia of indigenous streptomyces strains
Water Air and Soil Pollution, 2011Co-Authors: Maria Soledad Fuentes, Claudia S Benimeli, Maria J Amoroso, Juliana Maria SaezAbstract:The current study aimed to compare Lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of Lindane. Furthermore, the role of bacterial consortia of Lindane-degrading strains was examined in Lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to Lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and Lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L-1 Lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum Lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual Lindane concentration and SDA and could be a promising tool for Lindane biodegradation.
Claudia S Benimeli - One of the best experts on this subject based on the ideXlab platform.
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An Overview on Microbial Degradation of Lindane
Microbe-Induced Degradation of Pesticides, 2016Co-Authors: Juliana Maria Saez, Maria Soledad Fuentes, Maria J Amoroso, Analia Alvarez, Claudia S BenimeliAbstract:Lindane is a cyclic, saturated and highly chlorinated pesticide with a broad spectrum, which has been used worldwide for many decades to control a variety of pests, and also in human health and veterinary. Afterward, it has been demonstrated that Lindane and its isomers may cause serious damage to health in the short and long term. Besides, Lindane is known to be persistent in the environment and tends to bioaccumulate along the food chain. Thus, Lindane residues remain in the environment for a long time and have been recently found in water, soil, sediments, plants, and animals all over the world, and even in human fluids and tissues. In this context, nowadays, scientists, working all over the world, are involved in developing Lindane remediation technologies including physical, chemical, and biological techniques. This article provides updated information on the biologic degradation of Lindane using different microorganisms such as bacteria, fungi, and algae, under both aerobic and anaerobic conditions.
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enhanced Lindane removal from soil slurry by immobilized streptomyces consortium
International Biodeterioration & Biodegradation, 2014Co-Authors: Juliana Maria Saez, Claudia S Benimeli, Maria J Amoroso, Analia AlvarezAbstract:Abstract The aim of this work was to assess Lindane removal from soil slurry by a Streptomyces consortium immobilized in cloth sachets, at different inoculum, Lindane and slurry concentrations. In concentrated slurry (soil/water ratio of 2:3), the higher Lindane removal (35.3 mg Kg −1 ) was obtained with the medium inoculum (10 7 CFU g −1 ) and the highest Lindane concentration tested, at 7 days of incubation. Although, Lindane removal was also detected in abiotic controls, probably caused by pesticide adsorption to soil particles. Thus, these parameters were selected for evaluating the pesticide removal in diluted slurry (soil/water ratio of 1:4). After 14 days of incubation, 28.7 mg Kg −1 of Lindane were removed. Also, a phytotoxicity assay demonstrated that seeds growing on diluted slurries bioremediated during 7 and 14 days, showed an improvement in biological parameters, compared to those growing on non-bioremediated slurries. Thus, bioremediated slurries would not have toxic effects on lettuce seeds.
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simultaneous bioremediation of cr vi and Lindane in soil by actinobacteria
International Biodeterioration & Biodegradation, 2014Co-Authors: Marta Alejandra Polti, Claudia S Benimeli, Maria J Amoroso, Juan Daniel AparicioAbstract:Abstract Environments co-contaminated with metals and organic compounds are difficult to remediate. Actinobacteria is an important group of microorganisms found in soils, with high metabolic versatility and potential for bioremediation. In this paper, actinobacteria were used to remediate soil co-contaminated with Cr(VI) and Lindane. Five actinobacteria, tolerant to Cr(VI) and Lindane mixture were selected: Streptomyces spp. A5, A11, M7, and MC1, and Amycolatopsis tucumanensis DSM 45259. Sterilized soil samples were inoculated with actinobacteria strains, either individually or as a consortium, and contaminated with Cr(VI) and Lindane, either immediately or after 7 days of growth, and incubated at 30 °C during 14 days. All actinobacteria were able to grow and remove both contaminants, the consortium formed by Streptomyces spp. A5, M7, MC1, and A. tucumanensis showed the highest Cr(VI) removal, while Streptomyces sp. M7 produced the maximum Lindane removal. In non-sterile soil samples, Streptomyces sp. M7 and the consortium removed more than 40% of the Lindane, while Streptomyces sp. M7 demonstrated the greatest Cr(VI) removal. The most appropriate strategy for bioremediation of Cr(VI) and Lindane co-contaminated soils would be the inoculation with Streptomyces sp. M7.
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maize plants zea mays root exudates enhance Lindane removal by native streptomyces strains
International Biodeterioration & Biodegradation, 2012Co-Authors: Analia Alvarez, Claudia S Benimeli, Maria J Amoroso, M L YanezAbstract:The organochlorine pesticide (OP) Lindane was removed from minimal medium (MM) by two Streptomyces native strains, while growing on maize root exudates (REs) as a primary carbon and energy source. REs supported 55 and 35% of Lindane removal by Streptomyces sp. strains A5 and M7, respectively, corroborating the hypothesis that co-metabolism may be a plant/microbe interaction important to bioremediation. In addition, residual Lindane concentration was more than half of the amount in MM supplemented with glucose compared to MM supplemented with REs, suggesting that exudates could be more appropriate carbon source to support aerobic dehalogenation of the pesticide. Lindane-degrading activity was detected in REs, which could explain 42% of Lindane removal in REs-Lindane assay, without microorganisms. Because Streptomyces sp. A5 showed maximum biomass and the highest pesticide removal in REs-Lindane assay, it was found to be the most promising strain regarding their future application. These results showed that phytostimulation of OP-degrading actinobacteria by maize REs are therefore likely to be a successful strategy for the remediation of Lindane-contaminated environments.
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Lindane biodegradation by defined consortia of indigenous streptomyces strains
Water Air and Soil Pollution, 2011Co-Authors: Maria Soledad Fuentes, Claudia S Benimeli, Maria J Amoroso, Juliana Maria SaezAbstract:The current study aimed to compare Lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of Lindane. Furthermore, the role of bacterial consortia of Lindane-degrading strains was examined in Lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to Lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and Lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L-1 Lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum Lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual Lindane concentration and SDA and could be a promising tool for Lindane biodegradation.
Nandita Singh - One of the best experts on this subject based on the ideXlab platform.
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effect of temperature variation on Lindane dissipation and microbial activity in soil
Ecological Engineering, 2015Co-Authors: Vishal Tripathi, P C Abhilash, Nandita Singh, H B Singh, D D PatraAbstract:There is a general consensus that temperature variation can significantly affect the fate and behavior of pesticides and microbial activity in soil. Therefore, the present study was aimed to evaluate the effect of temperature variation on dissipation of Lindane from soil. The soil samples were spiked with four different concentrations of Lindane (5, 10, 15 and 20 mg kg−1) and were incubated at 28, 33, 40 and 48 °C for 45 days. The residual Lindane concentrations in soil, 50% dissipation rate of Lindane (DT50), microbial biomass carbon (MBC) as well as soil dehydrogenase activities were monitored periodically. Irrespective of the initial Lindane concentrations and exposure days, the increase in temperature significantly reduced the residual Lindane and increased the dissipation of Lindane from soil (p < 0.001). Similarly, the temperature increase from 28–48 °C significantly reduced the MBC content (p < 0.01) and soil dehydrogenase activity (p < 0.001). Most interestingly, the warming climate significantly reduced the DT50 days at 99.9% confidence level. This was more prominent at 48 °C (r2 = 0.981). Our study concludes that warming temperature can significantly reduce the microbial activity, soil enzymes as well as the dissipation rate of pesticides from soil. To the best of our knowledge, this is the first experimental report on the dissipation of Lindane under warming temperature. However, more studies are required to underpin the detailed physical, chemical and biological process involved in the dissipation and bioremediation of pesticides under warming temperature.
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phytoextraction and dissipation of Lindane by spinacia oleracea l
Ecotoxicology and Environmental Safety, 2014Co-Authors: Rama Kant Dubey, Vishal Tripathi, Nandita Singh, P C AbhilashAbstract:Abstract Remediation and management of organochlorine pesticide (OCPs) contaminated soil is becoming a global priority as they are listed in the Stockholm list of persistent organic pollutants (POPs) for global elimination. Lindane is a OCPs candidate recently included in the Stockholm list. However, India has an exemption to produce Lindane for malaria control. Because of its widespread use during the last few decades, Lindane contaminated soils are found in almost all parts of India. Since phytoremediation is widely acknowledged as an innovative strategy for the clean-up of contaminated soils; the present study was aimed to evaluate the phytoextraction and dissipation of Lindane by a leafy vegetable Spinacia oleracea L (Spinach). The test plant was grown in different concentrations of Lindane (5, 10, 15 and 20 mg kg−1) and harvested at 10, 30 and 45 days. At 45 days, the concentrations of Lindane in root and leaf of Spinach growing in four different concentrations were reached up to 3.5, 5.4, 7.6 and 12.3 mg kg−1 and 1.8, 2.2, 3 and 4.9 mg kg−1, respectively. There was a significant difference (p
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remediation of Lindane by jatropha curcas l utilization of multipurpose species for rhizoremediation
Biomass & Bioenergy, 2013Co-Authors: P C Abhilash, Bindu Singh, Pankaj Srivastava, Andreas Schaeffer, Nandita SinghAbstract:In the present study we demonstrate the rhizoremediation potential of a biodiesel plant Jatropha curcas L. against Lindane and discuss the field applicability of Jatropha based remediation techniques and future research prospects. Six different experimental approaches were conducted to evaluate the phytoremediation potential of Jatropha under glasshouse conditions. For this, Jatropha plants were grown in garden soil spiked with four increasing concentration of Lindane (5, 10, 15 and 20 mg kg(-1)) and harvested after 45, 180 and 300 d. One set of control plants were grown in Lindane free soil and another set of spiked soils were kept without Jatropha plants. At every harvesting, plant growth, Lindane accumulation in plant parts, residual Lindane concentration in soil as well as percentage Lindane dissipation from soil were calculated. After 300 d, the accumulation of Lindane in Jatropha grown in four simulated soils reached up to 5.42, 10.83, 15.95 and 20.85 mu g g(-1) plant dry matter, respectively. Correspondingly, the residual Lindane soil concentrations in the above four treatments were reduced to 89, 82, 77 and 72% with respect to the applied Lindane amounts, respectively. We conclude that Jatropha enhances the dissipation of Lindane in simulated soil and is useful for onsite remediation. (C) 2013 Elsevier Ltd. All rights reserved.
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Remediation of Lindane using engineered nanoparticles.
Journal of Biomedical Nanotechnology, 2011Co-Authors: Mugdha Srivastava, Purushothaman Chirakkuzhyil Abhilash, Nandita SinghAbstract:: Organochlorine pesticides (OCPs; aldrin, chlordane, DDT, dieldrin, endrin, heptachlor, mirex, toxaphene and hexachlorocyclohexane) are chemical pollutants found in all environmental media. There is an urgent need to stop the usage and develop innovative strategies for the remediation of contaminated soil and water. The present work was aimed to evaluate the (i) interaction of fullerene with Lindane and its role in the remediation of Lindane from contaminated systems and (ii) compare the interaction of fullerene with Lindane and trichloroethylene. Strong molecule-surface bonding of fullerene-Lindane complex than fullerene-TCE complex indicates that fullerene can be used as a potential nanoparticle for remediation of Lindane. However, toxicity and fate of nanoparticles is under investigation and more studies are needed before utilization of fullerene and other nanoparticles for phytoremediation.
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comparative bioremediation potential of four rhizospheric microbial species against Lindane
Chemosphere, 2011Co-Authors: P C Abhilash, Shubhi Srivastava, Nandita SinghAbstract:Abstract Four microbial species (Kocuria rhizophila, Microbacterium resistens, Staphylococcus equorum and Staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a Lindane contaminated environment and acclimatized in Lindane spiked media (5–100 μg mL−1). The isolated species were inoculated with soil containing 5, 50 and 100 mg kg−1 of Lindane and incubated at room temperature. Soil samples were collected periodically to evaluate the microbial dissipation kinetics, dissipation rate, residual Lindane concentration and microbial biomass carbon (MBC). There was a marked difference (p
P C Abhilash - One of the best experts on this subject based on the ideXlab platform.
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effect of temperature variation on Lindane dissipation and microbial activity in soil
Ecological Engineering, 2015Co-Authors: Vishal Tripathi, P C Abhilash, Nandita Singh, H B Singh, D D PatraAbstract:There is a general consensus that temperature variation can significantly affect the fate and behavior of pesticides and microbial activity in soil. Therefore, the present study was aimed to evaluate the effect of temperature variation on dissipation of Lindane from soil. The soil samples were spiked with four different concentrations of Lindane (5, 10, 15 and 20 mg kg−1) and were incubated at 28, 33, 40 and 48 °C for 45 days. The residual Lindane concentrations in soil, 50% dissipation rate of Lindane (DT50), microbial biomass carbon (MBC) as well as soil dehydrogenase activities were monitored periodically. Irrespective of the initial Lindane concentrations and exposure days, the increase in temperature significantly reduced the residual Lindane and increased the dissipation of Lindane from soil (p < 0.001). Similarly, the temperature increase from 28–48 °C significantly reduced the MBC content (p < 0.01) and soil dehydrogenase activity (p < 0.001). Most interestingly, the warming climate significantly reduced the DT50 days at 99.9% confidence level. This was more prominent at 48 °C (r2 = 0.981). Our study concludes that warming temperature can significantly reduce the microbial activity, soil enzymes as well as the dissipation rate of pesticides from soil. To the best of our knowledge, this is the first experimental report on the dissipation of Lindane under warming temperature. However, more studies are required to underpin the detailed physical, chemical and biological process involved in the dissipation and bioremediation of pesticides under warming temperature.
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phytoextraction and dissipation of Lindane by spinacia oleracea l
Ecotoxicology and Environmental Safety, 2014Co-Authors: Rama Kant Dubey, Vishal Tripathi, Nandita Singh, P C AbhilashAbstract:Abstract Remediation and management of organochlorine pesticide (OCPs) contaminated soil is becoming a global priority as they are listed in the Stockholm list of persistent organic pollutants (POPs) for global elimination. Lindane is a OCPs candidate recently included in the Stockholm list. However, India has an exemption to produce Lindane for malaria control. Because of its widespread use during the last few decades, Lindane contaminated soils are found in almost all parts of India. Since phytoremediation is widely acknowledged as an innovative strategy for the clean-up of contaminated soils; the present study was aimed to evaluate the phytoextraction and dissipation of Lindane by a leafy vegetable Spinacia oleracea L (Spinach). The test plant was grown in different concentrations of Lindane (5, 10, 15 and 20 mg kg−1) and harvested at 10, 30 and 45 days. At 45 days, the concentrations of Lindane in root and leaf of Spinach growing in four different concentrations were reached up to 3.5, 5.4, 7.6 and 12.3 mg kg−1 and 1.8, 2.2, 3 and 4.9 mg kg−1, respectively. There was a significant difference (p
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remediation of Lindane by jatropha curcas l utilization of multipurpose species for rhizoremediation
Biomass & Bioenergy, 2013Co-Authors: P C Abhilash, Bindu Singh, Pankaj Srivastava, Andreas Schaeffer, Nandita SinghAbstract:In the present study we demonstrate the rhizoremediation potential of a biodiesel plant Jatropha curcas L. against Lindane and discuss the field applicability of Jatropha based remediation techniques and future research prospects. Six different experimental approaches were conducted to evaluate the phytoremediation potential of Jatropha under glasshouse conditions. For this, Jatropha plants were grown in garden soil spiked with four increasing concentration of Lindane (5, 10, 15 and 20 mg kg(-1)) and harvested after 45, 180 and 300 d. One set of control plants were grown in Lindane free soil and another set of spiked soils were kept without Jatropha plants. At every harvesting, plant growth, Lindane accumulation in plant parts, residual Lindane concentration in soil as well as percentage Lindane dissipation from soil were calculated. After 300 d, the accumulation of Lindane in Jatropha grown in four simulated soils reached up to 5.42, 10.83, 15.95 and 20.85 mu g g(-1) plant dry matter, respectively. Correspondingly, the residual Lindane soil concentrations in the above four treatments were reduced to 89, 82, 77 and 72% with respect to the applied Lindane amounts, respectively. We conclude that Jatropha enhances the dissipation of Lindane in simulated soil and is useful for onsite remediation. (C) 2013 Elsevier Ltd. All rights reserved.
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comparative bioremediation potential of four rhizospheric microbial species against Lindane
Chemosphere, 2011Co-Authors: P C Abhilash, Shubhi Srivastava, Nandita SinghAbstract:Abstract Four microbial species (Kocuria rhizophila, Microbacterium resistens, Staphylococcus equorum and Staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a Lindane contaminated environment and acclimatized in Lindane spiked media (5–100 μg mL−1). The isolated species were inoculated with soil containing 5, 50 and 100 mg kg−1 of Lindane and incubated at room temperature. Soil samples were collected periodically to evaluate the microbial dissipation kinetics, dissipation rate, residual Lindane concentration and microbial biomass carbon (MBC). There was a marked difference (p
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withania somnifera dunal mediated dissipation of Lindane from simulated soil implications for rhizoremediation of contaminated soil
Journal of Soils and Sediments, 2010Co-Authors: P C Abhilash, Nandita SinghAbstract:Background, aim, and scope Lindane is an organochlorine chemical that has been used both as an agricultural insecticide and as a treatment for head lice and scabies. It is a neurotoxin that interferes with GABA neurotransmitter function. In humans, Lindane primarily affects the nervous system, liver, and kidneys and may be a carcinogen and/or endocrine disruptor. Currently, India is the largest consumer and producer of Lindane in the world. Due to its continuous use and indiscriminate industrial production, Lindane-contaminated soils are widespread in the country. Apart from India, historical Lindane production sites were found in Austria, France, Spain, Bulgaria and in China, Turkey, and the former USSR. Before 1984, Lindane was also manufactured in the German Democratic Republic, Poland, Yugoslavia, Romania, and Hungary; since then, all production has been stopped in Germany, Japan, The Netherlands, the UK, and the USA. Because of its worldwide use for more than 50 years, Lindane-contaminated soils can be found in most countries of the world. Although many countries have restricted or eliminated its usage, obsolete stock piles continue to pose a threat to various ecosystems and human health. Physical, chemical, and biological methods can all be used for the remediation of contaminated sites, but phytoremediation is now recognized as a cost-effective method for the decontamination of soil sites. The present study examines the potential of Withania somnifera Dunal (previously shown to accumulate Lindane from contaminated industrial area; Abhilash et al., Chemosphere 72:79–86, 2008) to take up Lindane (γ-HCH) and the subsequent plant-mediated dissipation of Lindane from an artificially contaminated soil.
Jaseetha Abdul Salam - One of the best experts on this subject based on the ideXlab platform.
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microbial enhanced Lindane removal by sugarcane saccharum officinarum in doped soil applications in phytoremediation and bioaugmentation
Journal of Environmental Management, 2017Co-Authors: Jaseetha Abdul Salam, Mohammed A A HathaAbstract:The aim of this study was to examine the effect of Lindane-degrading yeast on the growth and Lindane uptake by Saccharum sp., in doped garden soils. The rhizosphere of Saccharum plant was amended with yeast Candida VITJzN04 by root-inoculation. The bio-augment yeast was applied in two different forms viz., planktonic form and cells immobilized on sugarcane-bagasse, in the pot experiments. Garden soils (Lindane∼100 mg/kg) exposed to various treatments were monitored for a period of 30 days, for residual Lindane by gas-chromatography analysis. The Lindane-removal rates in soil were expressed in terms of half-life period and were recorded as 13.3 days (yeast), 43.3 days (Saccharum), 9.8 days (free yeast-plant) and 7.1 days (immobilized yeast-plant). Additionally, Candida sp., was also identified as a plant growth promoting yeast due to its ability to produce growth hormone and solubilize insoluble phosphates in the soil for better uptake by the plant species. Bio-stimulation of the soil with yeast immobilized on sugarcane bagasse further enhanced the total yeast activity in the soil which in turn had a positive influence on Lindane-removal. Combined treatment with bagasse immobilized yeast and plant showed the best Lindane degradation. Results suggested that the synergistic activity of plant and yeast resulted in fast and efficient degradation of Lindane. Thus, it can be concluded that Saccharum plant in combination with Candida VITJzN04 is an effective alternative for the conventional remediation strategies.
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Lindane degradation by candida vitjzn04 a newly isolated yeast strain from contaminated soil kinetic study enzyme analysis and biodegradation pathway
World Journal of Microbiology & Biotechnology, 2014Co-Authors: Jaseetha Abdul SalamAbstract:A new yeast strain was isolated from sugarcane cultivation field which was able to utilize Lindane as sole carbon source for growth in mineral medium. The yeast was identified and named as Candida sp. VITJzN04 based on a polyphasic approach using morphological, biochemical and 18S rDNA, D1/D2 and ITS sequence analysis. The isolated yeast strain efficiently degraded 600 mg L−1 of Lindane within 6 days in mineral medium under the optimal conditions (pH 7; temperature 30 °C and inoculum dosage 0.06 g L−1) with the least half-life of 1.17 days and degradation constant of 0.588 per day. Lindane degradation was tested with various kinetic models and results revealed that the reaction could be described best by first-order and pseudo first-order models. In addition, involvement of the enzymes viz. dechlorinase, dehalogenase, dichlorohydroquinone reductive dechlorinase, lignin peroxidase and manganese peroxidase was noted during Lindane degradation. Addition of H2O2 in the mineral medium showed 32 % enhancement of Lindane degradation within 3 days. Based on the metabolites identified by GC–MS and FTIR analysis, sequential process of Lindane degradation by Candida VITJzN04 was proposed. To the best of our knowledge, this is the first report of isolation and characterization of Lindane-degrading Candida sp. and elucidation of enzyme systems during the degradation process.
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biodegradation of Lindane using a novel yeast strain rhodotorula sp vitjzn03 isolated from agricultural soil
World Journal of Microbiology & Biotechnology, 2013Co-Authors: Jaseetha Abdul Salam, V LakshmiAbstract:Lindane is a notorious organochlorine pesticide due to its high toxicity, persistence in the environment and its tendency to bioaccumulate. A yeast strain isolated from sorghum cultivation field was able to use Lindane as carbon and energy source under aerobic conditions. With molecular techniques, it was identified and named as Rhodotorula strain VITJzN03. The effects of nutritional and environmental factors on yeast growth and the biodegradation of Lindane was investigated. The maximum production of yeast biomass along with 100 % Lindane mineralization was noted at an initial Lindane concentration of 600 mg l−1 within a period of 10 days. Lindane concentration above 600 mg l−1 inhibited the growth of yeast in liquid medium. A positive relationship was noted between the release of chloride ions and the increase of yeast biomass as well as degradation of Lindane. The calculated degradation rate and half life of Lindane were found to be 0.416 day−1 and 1.66 days, respectively. The analysis of the metabolites using GC–MS identified the formation of seven intermediates including γ-pentachlorocyclohexane(γ-PCCH), 1,3,4,6-tetrachloro-1,4-cyclohexadiene(1,4-TCCHdiene), 1,2,4-trichlorobenzene (1,2,4 TCB), 1,4-dichlorobenzene (1,4 DCB), chloro-cis-1,2-dihydroxycyclohexadiene (CDCHdiene), 3-chlorocatechol (3-CC) and maleylacetate (MA) derivatives indicating that Lindane degradation follows successive dechlorination and oxido-reduction. Based on the results of the present study, the possible pathway for Lindane degradation by Rhodotorula sp. VITJzN03 has been proposed. To the best of our knowledge, this is the first report on Lindane degradation by yeast which can serve as a potential agent for in situ bioremediation of medium to high level Lindane-contaminated sites.
