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Richard Wall - One of the best experts on this subject based on the ideXlab platform.
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Mitochondrial genomes of the sheep blowfly, Lucilia sericata, and the secondary blowfly, Chrysomya megacephala.
Medical and veterinary entomology, 2008Co-Authors: Jamie R. Stevens, H. West, Richard WallAbstract:This paper presents complete mitochondrial genomes for the sheep blowfly, Lucilia sericata (Meigen), and the secondary blowfly, Chrysomya megacephala (Fabricius). Both L. sericata and C. megacephala had standard dipteran-type mito- chondrial genome architectures and lengths of 15 945 bp and 15 831 bp, respectively. Additionally, C. megacephala possessed a tRNA duplication either side of the D-loop, as previously reported in another Chrysomya species, C. putoria ; this duplication ap- pears to be synapomorphic for the genus Chrysomya. As in other insect mitochondrial genomes, base compositions had a high AT content, with both genomes more than 76% AT-rich.
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Colour discrimination by the sheep blowfly, Lucilia sericata
Medical and veterinary entomology, 1996Co-Authors: Richard Wall, Ke SmithAbstract:. In laboratory trials designed to examine the alighting response of the blowfly Lucilia sericata Meigen to colour, yellow sticky cloth targets caught the largest number of both males and females, followed by pale blue, black, green, dark blue and red. The number caught by any colour showed a strong positive relationship with the percentage of its spectral reflectivity in the 450–580 nm wavelength band. In field trials, targets baited with the synthetic attractant ‘swormlure’ caught significantly fewer L.sericata than targets baited with liver and sodium sulphide, suggesting that the former bait is a relatively poor attractant for this species, at the release rates used in the present study. However, there was no effect of target colour on catch, neither was there any interaction between colour and odour bait type. The results, from both the laboratory and field trials, suggest that the strength of responses by Lsericata to visual cues are weak relative to responses to odour. Responses to hue are readily overridden by a range of factors such as brightness and contrast with the background and are therefore likely to be difficult to detect or manipulate reliably in trapping systems in the field.
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The reproductive output of the blowfly Lucilia sericata
Journal of Insect Physiology, 1993Co-Authors: Richard WallAbstract:Abstract The number of oocytes matured by the sheep blowfly Lucilia sericata Meigen was found to depend on female size and the amount of protein-rich material available. Given access to protein ad libitum in the laboratory, females matured an average of 223 (±12) oocytes per batch. However, female L. sericata collected from the field were not significantly different in size from laboratory-reared individuals and matured an average of 225 (±7) oocytes per batch. The size of adult females in the field showed no significant seasonal change. Hence, neither larval competition nor protein availability for vitellogenesis appear to be significant limiting factors for L. sericata in the field. The age of females collected from the field was assessed using a combination of the measurement of pteridine levels in the head and oocyte length. Computation of mortality rates from field age distributions gave estimates of 27–34% per day in 1991 at an average ambient temperature of 19°C and 16–17% per day in 1992 at 16°C. At these mortality rates mean lifetime reproductive output was estimated to be 44 eggs per female.
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Predicting the abundance of the blowfly Lucilia sericata (Diptera: Calliphoridae)
Bulletin of Entomological Research, 1993Co-Authors: Richard Wall, Nigel P. French, Kenton L. MorganAbstract:Populations of the blowfly Lucilia sericata Meigen in sheep pastures were monitored for three years (1990–1992) using sticky targets. A simulation model, developed previously from detailed analyses of the effects of temperature on the component stages of the life-cycle of L. sericata , was used to predict the pattern of seasonal abundance expected during these years at the temperatures observed. After inclusion of the effects of temperature over each catching interval, the model was able to explain 67%, 52% and 49% of the variance in catch in 1990, 1991 and 1992, respectively. The model was validated by using regression coefficients and constants derived from data collected over previous years to predict the expected catch in the following year. Up to 50% of the variance in catch was explained by this analysis. The results confirm value of the day-degree modelling approach for this blowfly species and suggest that such predictive models may play an important role in the development of strategic control for flystrike prevention.
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Ovarian development and pteridine accumulation for age determination in the blowfly Lucilia sericata
Journal of Insect Physiology, 1991Co-Authors: Richard Wall, P.a. Langley, Kenton L. MorganAbstract:Abstract The patterns of ovarian development and pteridine accumulation in the blowfly Lucilia sericata Meigen are described with reference to age, temperature and fly size. Formulae are presented which allow measurements of egg follicle length and pteridine fluorescence to be related to chronological age. The limitations associated with the two methods of age determination are considered. It is concluded that for L. sericata females, the techniques would be of maximal value if used in combination, with pteridine fluorescence levels being used to identify the broad age-range and the likely ovarian cycle and more exact estimates within this range obtained by ovarian dissection.
Jamie R. Stevens - One of the best experts on this subject based on the ideXlab platform.
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Mitochondrial genomes of the sheep blowfly, Lucilia sericata, and the secondary blowfly, Chrysomya megacephala.
Medical and veterinary entomology, 2008Co-Authors: Jamie R. Stevens, H. West, Richard WallAbstract:This paper presents complete mitochondrial genomes for the sheep blowfly, Lucilia sericata (Meigen), and the secondary blowfly, Chrysomya megacephala (Fabricius). Both L. sericata and C. megacephala had standard dipteran-type mito- chondrial genome architectures and lengths of 15 945 bp and 15 831 bp, respectively. Additionally, C. megacephala possessed a tRNA duplication either side of the D-loop, as previously reported in another Chrysomya species, C. putoria ; this duplication ap- pears to be synapomorphic for the genus Chrysomya. As in other insect mitochondrial genomes, base compositions had a high AT content, with both genomes more than 76% AT-rich.
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antibacterial properties of larval secretions of the blowfly Lucilia sericata
Medical and Veterinary Entomology, 2005Co-Authors: A. Kerridge, Hilary M Lappinscott, Jamie R. StevensAbstract:Abstract. The antibacterial properties of secretions aseptically collected from larvae of the greenbottle fly Lucilia sericata (Meigen) (Diptera: Calliphoridae) were examined. These investigations revealed the presence of small (<1 kDa) antibacterial factor(s) within the larval secretions, active against a range of bacteria. These include the Gram-positive Staphylococcus aureus, both methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA), Streptococcus pyogenes and to a lesser extent the Gram-negative Pseudomonas aeruginosa. These secretions were shown to be highly stable as a freeze-dried preparation and, considering the activity against organisms typically associated with clinical infection, may be a source of novel antibiotic-like compounds that may be used for infection control and in the fight against MRSA.
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Antibacterial properties of larval secretions of the blowfly, Lucilia sericata.
Medical and veterinary entomology, 2005Co-Authors: A. Kerridge, Hilary M. Lappin-scott, Jamie R. StevensAbstract:Abstract. The antibacterial properties of secretions aseptically collected from larvae of the greenbottle fly Lucilia sericata (Meigen) (Diptera: Calliphoridae) were examined. These investigations revealed the presence of small (
Jeffery K Tomberlin - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Sterilized Artificial Diets for Mass Rearing the Lucilia sericata (Diptera: Calliphoridae).
Journal of medical entomology, 2017Co-Authors: Le Zheng, Tawni L Crippen, Alan R. Dabney, Alex Gordy, Jeffery K TomberlinAbstract:The impact of six sterilized diets (blood-yeast agar diet, decomposed beef liver diet, powdered beef liver diet, powdered fish diet, milk-based diet, and a chemically defined diet) on Lucilia sericata (Meigen) larvae reared at three densities (10 larvae, 20 larvae, and 40 larvae on 20 g diet) was determined in comparison to fresh beef liver as a control. Specifically, the effects of these diets on the following traits of L. sericata were measured: 1) pupal weight, 2) pupation percentage, 3) eclosion percentage, as well as 4) adult longevity. The experiment included two trials with five technical replicates in each. Lucilia sericata did not successfully develop on the powdered fish, milk-based, or chemically defined diets. Overall, the liver-based diets (decomposed and powdered) resulted in the most similar fly development to the fresh beef liver. Larvae reared on blood-yeast agar diet resulted in a significantly (increased 20.56% ± 8.09%) greater pupation rate than those reared on the decomposed and powdered beef liver diets. Pupae from larvae fed the fresh beef liver were significantly larger (6.27 ± 1.01 mg, 4.05 ± 0.94 mg larger, respectively) than those reared on the blood-yeast agar diet, decomposed beef liver, and powdered beef liver diets. Overall, results revealed larvae reared on sterilized liver-based diets resulted in traits similar to those raised on fresh beef liver. Owing to low costs the sterile liver-based diets could be produced and used with limited infrastructure and economic incomes.
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genome sequence of a providencia stuartii strain isolated from Lucilia sericata salivary glands
Genome Announcements, 2017Co-Authors: Ye Yuan, Shuhua Fu, David K Visi, Michael S. Allen, Eric M Benbow, Tawni L Crippen, Jeffery K Tomberlin, Aaron M TaroneAbstract:ABSTRACT We present here the draft genome sequence of a Providencia stuartii strain, derived from the salivary glands of larval Lucilia sericata, a common blow fly important to forensic, medical, and veterinary science. The genome sequence will help dissect coinfections involving P. stuartii and Proteus mirabilis, as well as blow fly–bacteria interactions.
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Genome Sequence of a Proteus mirabilis Strain Isolated from the Salivary Glands of Larval Lucilia sericata.
Genome announcements, 2016Co-Authors: Ye Yuan, David K Visi, Michael S. Allen, Tawni L Crippen, Jeffery K Tomberlin, Yu Zhang, M. Eric Benbow, Sing-hoi Sze, Aaron M TaroneAbstract:ABSTRACT We announce a draft genome sequence of a Proteus mirabilis strain derived from Lucilia sericata salivary glands. This strain is demonstrated to attract and induce oviposition by L. sericata, a common blow fly important to medicine, agriculture, and forensics. The genome sequence will help dissect interkingdom communication between the species.
A. Kerridge - One of the best experts on this subject based on the ideXlab platform.
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antibacterial properties of larval secretions of the blowfly Lucilia sericata
Medical and Veterinary Entomology, 2005Co-Authors: A. Kerridge, Hilary M Lappinscott, Jamie R. StevensAbstract:Abstract. The antibacterial properties of secretions aseptically collected from larvae of the greenbottle fly Lucilia sericata (Meigen) (Diptera: Calliphoridae) were examined. These investigations revealed the presence of small (<1 kDa) antibacterial factor(s) within the larval secretions, active against a range of bacteria. These include the Gram-positive Staphylococcus aureus, both methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA), Streptococcus pyogenes and to a lesser extent the Gram-negative Pseudomonas aeruginosa. These secretions were shown to be highly stable as a freeze-dried preparation and, considering the activity against organisms typically associated with clinical infection, may be a source of novel antibiotic-like compounds that may be used for infection control and in the fight against MRSA.
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Antibacterial properties of larval secretions of the blowfly, Lucilia sericata.
Medical and veterinary entomology, 2005Co-Authors: A. Kerridge, Hilary M. Lappin-scott, Jamie R. StevensAbstract:Abstract. The antibacterial properties of secretions aseptically collected from larvae of the greenbottle fly Lucilia sericata (Meigen) (Diptera: Calliphoridae) were examined. These investigations revealed the presence of small (
Yamni Nigam - One of the best experts on this subject based on the ideXlab platform.
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multiple actions of Lucilia sericata larvae in hard to heal wounds
BioEssays, 2013Co-Authors: Gwendolyn Cazander, Yamni Nigam, D I Pritchard, Willi Jung, Peter H NibberingAbstract:In Europe ≈15,000 patients receive larval therapy for wound treatment annually. Over the past few years, clinical studies have demonstrated the success of larvae of Lucilia sericata as debridement agents. This is based on a combination of physical and biochemical actions. Laboratory investigations have advanced our understanding of the biochemical mechanisms underlying the beneficial effects of larval secretions, including removal of dead tissue, reduction of the bacterial burden, and promotion of tissue regeneration. The present article summarizes our current understanding of the microbiological, immunological, and wound healing actions of larval therapy, and the molecules involved in these beneficial effects. Future studies will focus on the isolation, identification, and (pre)clinical testing of the effective molecules of L. sericata larvae. These molecules may be candidates for the development of new agents for the treatment of several infectious and inflammatory diseases, including chronic wounds.
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Lucilia sericata Chymotrypsin Disrupts Protein Adhesin-Mediated Staphylococcal Biofilm Formation
Applied and environmental microbiology, 2012Co-Authors: Llinos G Harris, Yamni Nigam, Dietrich Mack, James Sawyer, David I. PritchardAbstract:ABSTRACT Staphylococcus aureus and Staphylococcus epidermidis biofilms cause chronic infections due to their ability to form biofilms. The excretions/secretions of Lucilia sericata larvae (maggots) have effective activity for debridement and disruption of bacterial biofilms. In this paper, we demonstrate how chymotrypsin derived from maggot excretions/secretions disrupts protein-dependent bacterial biofilm formation mechanisms.
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The Physiology of Wound Healing by the Medicinal Maggot, Lucilia sericata
Advances in Insect Physiology, 2010Co-Authors: Yamni Nigam, Alyson Bexfield, Edward G. Dudley, A. Elizabeth Bond, Julie A Evans, Jane JamesAbstract:Abstract The larvae of Lucilia sericata (the medicinal maggot) have been exploited to undertake many applications, from their use in the management of chronic, non-healing wounds (larval therapy), to their assistance in forensic investigations and analysis. This current review describes these various applications and discusses the recent scientific and clinical developments supporting their use in wound healing and management. The review includes aspects such as their role in wound debridement, disinfection properties and biofilm disruption, as well as their potential role in the acceleration of wound healing. Also considered is the study of novel therapeutic compounds and biological therapies derived from this species. From a clinical perspective, recent studies that have been concerned with the efficacy and value of maggot debridement therapy are summarised and discussed.
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disruption of staphylococcus epidermidis biofilms by medicinal maggot Lucilia sericata excretions secretions
International Journal of Artificial Organs, 2009Co-Authors: Llinos G Harris, Alyson Bexfield, Norman A Ratcliffe, Holger Rohde, Yamni Nigam, Dietrich MackAbstract:Chronic infections are commonly associated with biofilms formed by bacteria such as Staphylococcus epidermidis. With the increase in antibiotic resistant bacteria, maggot debridement therapy has been reintroduced for the treatment of chronic wounds. Studies have shown that the excretion/secretions (ES) of Lucilia sericata larvae (maggots) contain many bioactive compounds which may contribute to the efficacy of maggot therapy. The present study evaluates the effect of L. sericata ES on the formation and disruption of S. epidermidis 7457 and 5179-R1 biofilms. These strains employ either polysaccharide intercellular adhesin (PIA) or accumulation associated protein (Aap) for intercellular adhesion.A semiquantitative biofilm assay was used to measure the formation/disruption of S. epidermidis 7457 and 5179-R1 biofilms by ES. ES activity was characterized according to concentration, incubation time and temperature, thermal stability, and size. Immunofluorescence microscopy was used to ascertain the effect of ES...
