The Experts below are selected from a list of 4431 Experts worldwide ranked by ideXlab platform
Hongwei Liu - One of the best experts on this subject based on the ideXlab platform.
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sesquiterpenes and polyphenols with glucose uptake stimulatory and antioxidant activities from the Medicinal Mushroom sanghuangporus sanghuang
Chinese Journal of Natural Medicines, 2021Co-Authors: Jinjin Zhang, Baosong Chen, Huanqin Dai, Jinwei Ren, Liwei Zhou, Hongwei LiuAbstract:A chemical investigation on the fermentation products of Sanghuangporus sanghuang led to the isolation and identification of fourteen secondary metabolites ( 1 − 14 ) including eight sesquiterpenoids ( 1 − 8 ) and six polyphenols ( 9 − 14 ). Compounds 1 − 3 were sesquiterpenes with new structures which were elucidated based on NMR spectroscopy, high resolution mass spectrometry (HRMS) and electronic circular dichroism (ECD) data. All the isolates were tested for their stimulation effects on glucose uptake in insulin-resistant HepG2 cells, and cellular antioxidant activity. Compounds 9 − 12 were subjected to molecular docking experiment to primarily evaluate their anti-coronavirus (SARS-CoV-2) activity. As a result, compounds 9 − 12 were found to increase the glucose uptake of insulin-resistant HepG2 cells by 18.1%, 62.7%, 33.7% and 21.4% at the dose of 50 μmol·L−1, respectively. Compounds 9 − 12 also showed good cellular antioxidant activities with CAA50 values of 12.23, 23.11, 5.31 and 16.04 μmol·L−1, respectively. Molecular docking between COVID-19 Mpro and compounds 9 − 12 indicated potential SARS-CoV-2 inhibitory activity of these four compounds. This work provides new insights for the potential role of the Medicinal Mushroom S. sanghuang as drugs and functional foods.
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A novel polycyclic meroterpenoid with aldose reductase inhibitory activity from Medicinal Mushroom Ganoderma leucocontextum.
The Journal of antibiotics, 2017Co-Authors: Jinjin Zhang, Hongyu Chen, Kai Wang, Weiping Xiong, Li Bao, Hongwei LiuAbstract:A novel polycyclic meroterpenoid with aldose reductase inhibitory activity from Medicinal Mushroom Ganoderma leucocontextum
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lanostane triterpenes from the tibetan Medicinal Mushroom ganoderma leucocontextum and their inhibitory effects on hmg coa reductase and α glucosidase
Journal of Natural Products, 2015Co-Authors: Kai Wang, Weiping Xiong, Li Bao, Junjie Han, W Wang, Wenbing Yin, Hongwei LiuAbstract:Sixteen new lanostane triterpenes, ganoleucoins A-P (1-16), together with 10 known tripterpenes (17-26), were isolated from the cultivated fruiting bodies of Ganoderma leucocontextum, a new member of the Ganoderma lucidum complex. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical transformation. The inhibitory effects of 1-26 on HMG-CoA reductase and α-glucosidase were tested in vitro. Compounds 1, 3, 6, 10-14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity against HMG-CoA reductase than the positive control atorvastatin. Compounds 13, 14, and 16 presented potent inhibitory activity against α-glucosidase from yeast with IC₅₀ values of 13.6, 2.5, and 5.9 μM, respectively. In addition, the cytotoxicity of 1-26 was evaluated against the K562 and PC-3 cell lines by the MTT assay. Compounds 1, 2, 6, 7, 10, 12, 16, 18, and 25 exhibited cytotoxicity against K562 cells with IC₅₀ values in the range 10-20 μM. Paclitaxel was used as the positive control with an IC₅₀ value of 0.9 μM. This is the first report of secondary metabolites from this Medicinal Mushroom.
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six new 3 4 seco 27 norlanostane triterpenes from the Medicinal Mushroom ganoderma boninense and their antiplasmodial activity and agonistic activity to lxrβ
ChemInform, 2015Co-Authors: Li Bao, Chen Sun, Bing Zhou, Hongwei LiuAbstract:Ganoboninones A-F (I)-(IV) possessing an interesting tetradecahydrobenzo[4,5]indeno[1,7a-c]furan backbone are isolated along with three known compounds, ganoboninketals A-C, from the fruiting bodies of the Medicinal Mushroom Ganoderma boninense.
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six new 3 4 seco 27 norlanostane triterpenes from the Medicinal Mushroom ganoderma boninense and their antiplasmodial activity and agonistic activity to lxrβ
Tetrahedron, 2015Co-Authors: Li Bao, Chen Sun, Bing Zhou, Hongwei LiuAbstract:Abstract Six new 3,4-seco-27-norlanostane triterpenes, ganoboninones A–F (1–6), as well as three known compounds ganoboninketals A–C (7–9), were isolated from the fruiting bodies of the Medicinal Mushroom Ganoderma boninense. The structures of 1–6 were established by extensive spectroscopic analysis. Compounds 1–6 are new 3,4-seco-27-norlanostane triterpenoids featuring with an interesting tetradecahydrobenzo[4,5]indeno[1,7a-c]furan backbone. The absolute configurations in 1–6 were determined by CD spectral analysis, and ECD calculation. In an in vitro anti-Plasmodium falciparum bioassay, compounds 1, 2, and 6 showed antimalarial effects with IC50 values of 27.36, 15.68, and 2.03 μM, respectively. In a transactivation assay, compounds 5, 7, 8, and 9 showed agonistic activity to LXRβ with EC50 value of 203.00, 8.32, 257.00, and 86.70 nM, respectively.
Jianjiang Zhong - One of the best experts on this subject based on the ideXlab platform.
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impacts of calcium signal transduction on the fermentation production of antitumor ganoderic acids by Medicinal Mushroom ganoderma lucidum
Biotechnology Advances, 2012Co-Authors: Jianjiang ZhongAbstract:Recently signal transduction engineering of secondary metabolism is receiving great interest as a powerful tool towards efficient production of valuable secondary metabolites. This work found that the calcineurin-signal transduction was significant to triterpene biosynthesis by higher fungus (Mushroom). Addition of calcium ion (at 10mM) to static liquid cultures of Ganoderma lucidum, a famous traditional Medicinal Mushroom, was proved as a useful strategy to enhance the production of antitumor ganoderic acids (GAs), which resulted in 3.7-, 2.6-, 4.5-, 3.2- and 3.8-fold improvement of total GAs, individual GA-Mk, -T, -S, and -Me, respectively. Experiments using Ca2+ sensor inhibitors indicated the involvement of calcineurin signal in regulating GAs biosynthesis. Quantitative gene transcription analysis revealed that the expression levels of genes of GAs biosynthesis and Ca2+ sensor were up-regulated with calcium addition while down-regulated under the inhibitors addition, suggesting that higher GAs production may be resulted from higher expressions of those genes. Based on the results obtained, a possible model on the effect of external calcium ion on the GAs biosynthesis via calcineurin signal transduction pathway was proposed.
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polysaccharides from the Medicinal Mushroom cordyceps taii show antioxidant and immunoenhancing activities in a d galactose induced aging mouse model
Evidence-based Complementary and Alternative Medicine, 2012Co-Authors: Jianhui Xiao, Daimin Xiao, Daixiong Chen, Yu Xiao, Zongqi Liang, Jianjiang ZhongAbstract:Cordyceps taii, an edible Medicinal Mushroom native to south China, is recognized as an unparalleled resource of healthy foods and drug discovery. In the present study, the antioxidant pharmacological properties of C. taii were systematically investigated. In vitro assays revealed the scavenging activities of the aqueous extract and polysaccharides of C. taii against various free radicals, that is, 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, and superoxide anion radical. The EC50 values for superoxide anion-free radical ranged from 2.04 mg/mL to 2.49 mg/mL, which was at least 2.6-fold stronger than that of antioxidant thiourea. The polysaccharides also significantly enhanced the antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase) and markedly decreased the malondialdehyde production of lipid peroxidation in a D-galactose-induced aging mouse model. Interestingly, the immune function of the administration group was significantly boosted compared with the D-galactose-induced aging model group. Therefore, the C. taii polysaccharides possessed potent antioxidant activity closely associated with immune function enhancement and free radical scavenging. These findings suggest that the polysaccharides are a promising source of natural antioxidants and antiaging drugs. Consequently, a preliminary chemical investigation was performed using gas chromatography-mass spectroscopy and revealed that the polysaccharides studied were mainly composed of glucose, mannose, and galactose. Fourier-transform infrared spectra also showed characteristic polysaccharide absorption bands.
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significant effect of nh4 on cordycepin production by submerged cultivation of Medicinal Mushroom cordyceps militaris
Enzyme and Microbial Technology, 2006Co-Authors: Xianbing Mao, Jianjiang ZhongAbstract:Abstract Effects of nitrogen sources on cell growth and cordycepin production by submerged cultivation of Cordyceps militaris, a traditional Chinese Medicinal Mushroom, were investigated. In complex medium, peptone was identified as the best nitrogen source for cordycepin biosynthesis. A study using chemically defined medium indicated that NH4+ played an important role in cordycepin biosynthesis. To enhance cordycepin production, fed-batch culture with NH4+ feeding was conducted in complex medium with peptone. By optimizing the feeding time and feeding amount of NH4+, a maximal cordycepin concentration of 420.5 ± 15.1 mg/l was obtained, which was 70% higher than that in batch cultivation. The fed-batch cultivation process was also demonstrated in a 3.5 l stirred tank bioreactor and the cordycepin production was increased from 208.8 ± 7.6 mg/l in batch culture to 346.1 ± 3.6 mg/l in fed-batch culture. The information is useful for large-scale efficient production of cordycepin by the Mushroom cultures.
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optimization of carbon source and carbon nitrogen ratio for cordycepin production by submerged cultivation of Medicinal Mushroom cordyceps militaris
Process Biochemistry, 2005Co-Authors: Xianbing Mao, Titiporn Eksriwong, Somchai Chauvatcharin, Jianjiang ZhongAbstract:Abstract Effects of various carbon sources and carbon/nitrogen ratios on production of a useful bioactive metabolite, cordycepin (3′-deoxyadenosine), by submerged cultivation of a Chinese traditional Medicinal Mushroom Cordyceps militaris were investigated in shake flasks. The carbon sources examined were lactose, sucrose, glucose, fructose, galactose, maltose and xylose, and glucose was found to be most favourable to cordycepin production, whereas cells grew best in galactose medium. The dry cell weight (DW) was increased with an increase in initial glucose concentration within the range of 25–70 g/l as investigated. The highest cordycepin production, i.e. 245.7 ± 4.4 mg/l on day 18, was obtained in medium containing 40 g glucose/l. To enhance further the cordycepin production, the effect of carbon/nitrogen ratios was studied using central composite design and response surface analysis. The maximum cordycepin production and productivity of 345.4 ± 8.5 mg/l and 19.2 ± 0.5 mg/l per day were achieved in medium with optimized carbon and nitrogen sources, i.e. 42.0 g glucose/l and 15.8 g peptone/l. The information obtained is helpful for the hyperproduction of cordycepin by submerged cultivation of C. militaris on a large scale.
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hyperproduction of cordycepin by two stage dissolved oxygen control in submerged cultivation of Medicinal Mushroom cordyceps militaris in bioreactors
Biotechnology Progress, 2004Co-Authors: Xianbing Mao, Jianjiang ZhongAbstract:Effect of oxygen supply on cordycepin production was investigated in submerged cultivation of Cordyceps militaris, a famous traditional Chinese Medicinal Mushroom, in a 5-L turbine-agitated bioreactor (TAB). Initial volumetric oxygen transfer coefficient (kLa) within the range of 11.5-113.8 h(-1) had significant influence on cordycepin production. The highest cordycepin concentration of 167.5 mg/L was obtained at an initial kLa value of 54.5 h(-1), where a moderate dissolved oxygen (DO) pattern was observed throughout cultivation. The possible correlation between cordycepin production and DO level was explored by DO control experiments, and the results showed that DO within the range of 10-80% of air saturation greatly affected the cultivation process. To obtain a high specific cordycepin formation rate (rho) throughout cultivation, a two-stage DO control strategy was developed based on the analysis of the relationship of rho and DO. That is, DO was controlled at 60% from the beginning of cultivation and then shifted to a lower control level of 30% when rho started to decrease. As a result, a high cordycepin production of 201.1 mg/L and a high productivity of 15.5 mg/(L.d) were achieved, which was enhanced by about 15% and 30% compared to the highest titers obtained in conventional DO control experiments, respectively. The proposed DO control strategy was also applied to a recently developed 5-L centrifugal impeller bioreactor (CIB) with cordycepin production and productivity titers of 188.3 mg/L and 14.5 mg/(L.d). Furthermore, the scale-up of the two-stage DO control process from 5-L CIB to 30-L CIB was successfully demonstrated. The work is useful for the efficient large-scale production of bioactive metabolites by Mushroom cultures.
Jean-michel Savoie - One of the best experts on this subject based on the ideXlab platform.
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The genetic linkage map of the Medicinal Mushroom Agaricus subrufescens reveals highly conserved macrosynteny with the congeneric species Agaricus bisporus
G3, 2016Co-Authors: Marie Foulongne-oriol, Cathy Spataro, Magalie Moinard, Philippe Callac, Manuela Rocha De Brito, Eustáquio Souza Dias, Delphine Cabannes, Aurélien Clement, Jean-michel SavoieAbstract:Comparative linkage mapping can rapidly facilitate the transfer of genetic information from model species to orphan species. This macrosynteny analysis approach has been extensively used in plant species, but few example are available in fungi, and even fewer in Mushroom crop species. Among the latter, the Agaricus genus comprises the most cultivable or potentially cultivable species. Agaricus bisporus, the button Mushroom, is the model for edible and cultivable Mushrooms. We have developed the first genetic linkage map for the basidiomycete A. subrufescens, an emerging Mushroom crop known for its therapeutic properties and potential Medicinal applications. The map includes 202 markers distributed over 16 linkage groups (LG), and covers a total length of 1701 cM, with an average marker spacing of 8.2 cM. Using 96 homologous loci, we also demonstrated the high level of macrosynteny with the genome of A. bisporus. The 13 main LG of A. subrufescens were syntenic to the 13 A. bisporus chromosomes. A disrupted synteny was observed for the three remaining A. subrufescens LG. Electronic mapping of a collection of A. subrufescens expressed sequence tags on A. bisporus genome showed that the homologous loci were evenly spread, with the exception of a few local hot or cold spots of homology. Our results were discussed in the light of Agaricus species evolution process. The map provides a framework for future genetic or genomic studies of the Medicinal Mushroom A. subrufescens.
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Development of polymorphic microsatellite markers issued from pyrosequencing technology for the Medicinal Mushroom Agaricus subrufescens
FEMS Microbiology Letters, 2012Co-Authors: Marie Foulongne-oriol, Cathy Spataro, Magalie Moinard, Philippe Callac, Delphine Cabannes, Jean-michel SavoieAbstract:The recently described procedure of microsatellite-enriched library pyrosequencing was used to isolate microsatellite loci in the gourmet and Medicinal Mushroom Agaricus subrufescens. Three hundred and five candidate loci containing at least one simple sequence repeats (SSR) locus and for which primers design was successful, were obtained. From a subset of 95 loci, 35 operational and polymorphic SSR markers were developed and characterized on a sample of 14 A.similar to subrufescens genotypes from diverse origins. These SubSSR markers each displayed from two to 10 alleles with an average of 4.66 alleles per locus. The observed heterozygosity ranged from 0 to 0.71. Several multiplex combinations can be set up, making it possible to genotype up to six markers easily and simultaneously. Cross-amplification in some closely congeneric species was successful for a subset of loci. The 35 microsatellite markers developed here provide a highly valuable molecular tool to study genetic diversity and reproductive biology of A.similar to subrufescens.
Dean Guo - One of the best experts on this subject based on the ideXlab platform.
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pharmacokinetic studies of ganoderic acids from the lingzhi or reishi Medicinal Mushroom ganoderma lucidum agaricomycetes by lc ms ms
International Journal of Medicinal Mushrooms, 2016Co-Authors: Chunru Cheng, Jie Ding, Yi Yang, Xinyong Liang, Dean Guo, Min Yang, Shuhong GuanAbstract:Ganoderma lucidum is a famous Medicinal Mushroom that has been widely used in clinical practice and as a dietary supplementa. The triterpenoid ganoderic acids are the main constituents of G. lucidum. To determine the pharmacokinetic characteristics of ganoderic acids, we developed and validated a sensitive and selective liquid chromatography-tandem mass spectrometry method to determine simultaneously the concentration of 4 representative ganoderic acids in rat plasma after oral administration of the extract from G. lucidum. Because of the similarity of their chemical structures, the 4 components exhibited similar pharmacokinetic behaviors in some aspects. However, some of the pharmacokinetic parameters and the reabsorption peaks in the plasma concentration-time curves of ganoderic acids B and E after oral administration of the extract were different from those of ganoderic acids D and A because of the metabolic transformation among the ganoderic acids. These results increase our knowledge about the use of G. lucidum.
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comprehensive chemical analysis of triterpenoids and polysaccharides in the Medicinal Mushroom antrodia cinnamomea
RSC Advances, 2015Co-Authors: Xue Qiao, Dean Guo, Wei Song, Qi Wang, Kedi Liu, Zhengxiang Zhang, Lina Liang, Yewmin TzengAbstract:Antrodia cinnamomea is a precious Medicinal Mushroom used in adjuvant treatments of cancer. Triterpenoids (25R/S-ergostane epimers and Δ7,9/Δ8-lanostanes) and polysaccharides are its major bioactive constituents. Quality control of this Mushroom is difficult, due to a lack of effective analytical methods. In this study, a series of methods were established to analyze A. cinnamomea. The ergostane- and lanostane-type triterpenoids were extracted by 50% and 100% methanol, respectively, and were then analyzed by ultra-high performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC/DAD/qTOF-MS). In total 49 compounds, including 34 ergostanes and 10 lanostanes, were characterized by comparing with 31 reference standards or by analyzing the UV and MS spectral data. Furthermore, the contents of 18 major triterpenoids, including 10 ergostanes and 8 lanostanes, were determined by UPLC/UV or supercritical fluid chromatography coupled with mass spectrometry (SFC/MS, for 25R/S-antcin A) within 16 min. This is the first time that the contents of pure optical forms for 5 pairs of 25R- and 25S-ergostane epimers (antcins A, B, C, H, and K) were determined. On the other hand, the polysaccharides in A. cinnamomea were analyzed by ion chromatography coupled with pulsed amperometric detection (IC/PAD) after acid hydrolysis, and the contents of 7 monosaccharides were determined. Finally, the established methods were applied to the analysis of 15 batches of A. cinnamomea. Samples derived from different cultivation techniques could be distinguished according to the contents of triterpenoids. The UPLC/UV, SFC/MS and IC/PAD methods established in this work provided powerful tools to evaluate the quality of A. cinnamomea.
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separation of 25r s ergostane triterpenoids in the Medicinal Mushroom antrodia camphorata using analytical supercritical fluid chromatography
Journal of Chromatography A, 2014Co-Authors: Xue Qiao, Yewmin Tzeng, Yun Huang, Dean GuoAbstract:Ergostanes are major bioactive constituents of the Medicinal Mushroom Antrodia camphorata. These tetracyclic triterpenoids usually occur as 25R/S epimeric pairs, which renders their chromatographic separation difficult. In this study, we used analytical supercritical-fluid chromatography (SFC) to separate seven pairs of 25R/S-ergostanes from A. camphorata. The (R)- and (S)-forms for each of the seven pairs could be well resolved (Rs>1.3) on a Chiralcel OJ-H column (4.6×250mm, 5μm, chiral), eluted by 10% MeOH in CO2 at 2mL/min with a back pressure of 120bar and a column temperature of 40°C. Particularly, this chiral-SFC method could rapidly and efficiently separate low-polarity epimers like antcin A and antcin B, which were very difficult for RP-HPLC. A 3-min preparative-scale method was established to purify (25S)- and (25R)-antcin A for the first time. However, OJ-H column suffered from peak overlapping of different pairs of ergostanes. We found that Princeton 2-ethylpyridine column (2-EP, 4.6×250mm, 3μm, achiral) could effectively separate different pairs, although the resolutions for 25-R/S forms of each epimeric pair were not as good as OJ-H column. Meanwhile, all the (25S)-forms showed stronger retentions than the corresponding (25R)-epimers on the 2-EP column. These results demonstrated different selectivity of chiral- and achiral-SFC in separating 25R/S-ergostane epimers. Aside from high separation efficiency, SFC also showed advantage over HPLC in short analysis time and low consumption of organic solvents. Finally, both OJ-H and 2-EP columns were used on analytical SFC to separate 25R/S-ergostanes in an extract of A. camphorata.
Martin Hofrichter - One of the best experts on this subject based on the ideXlab platform.
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laccase from the Medicinal Mushroom agaricus blazei production purification and characterization
Applied Microbiology and Biotechnology, 2005Co-Authors: Rene Ullrich, Le Mai Huong, Nguyen Lan Dung, Martin HofrichterAbstract:The Medicinal Mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l−1) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l−1) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic steps, including anion and cation exchanging. A. blazei laccase was expressed as a single protein with a molecular mass of 66 kDa and an isoelectric point of 4.0. Spectroscopic analysis of the purified enzyme confirmed that it belongs to the “blue copper oxidases”. The enzyme’s pH optimum for 2,6-dimethoxyphenol (DMP) and syringaldazine was pH 5.5; but for 2,2′-azino-bis(3-ethylthiazoline-6-sulfonate) (ABTS) no distinct pH optimum was observed (highest activity at the lowest pH tested). Purified laccase was stable at 20°C, pH 7.0 and pH 3.0, but rapidly lost its activity at 40°C or pH 10. Sodium chloride strongly inhibited the enzyme activity, although the inhibition was completely reversible. The following kinetic constants were determined (Km, kcat): 63 μM, 21 s−1 for ABTS, 4 μM, 5 s−1 for syringaldazine, 1,026 μM, 15 s−1 for DMP and 4307 μM, 159 s−1 for guaiacol. The results show that—in addition to the wood-colonizing white-rot fungi—the typical litter-decomposing basidiomycetes can also produce high titers of laccase in suitable liquid media.
