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Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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fabrication of a biomimetic zeinpda Nanofibrous Scaffold impregnated with bmp 2 peptide conjugated tio2 nanoparticle for bone tissue engineering
Journal of Tissue Engineering and Regenerative Medicine, 2018Co-Authors: Sumathy Babitha, Michal Marcin Dykas, Kingshuk Poddar, Meenakshi Annamalai, Jayarama Reddy Venugopal, Thirumalai Venkatesan, Surajit Saha, Seeram Ramakrishna, Purna Sai KorrapatiAbstract:A biomimetic Zein polydopamine based nanofiber Scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to titanium dioxide nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nanotopographical stimulation of electrospun Zein polydopamine nanofiber were examined for its enhanced osteogenic expression of human fetal osteoblast cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization, and differentiation could be attributed to its highly interconnected Nanofibrous matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated Nanofibrous Scaffold possess better cell-biomaterial interactions. These promising results demonstrate the potential of the composite Nanofibrous Scaffold as an effective biomaterial substrate for bone regeneration.
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peptide modified Nanofibrous Scaffold promotes human mesenchymal stem cell proliferation and long term passaging
Materials Science and Engineering: C, 2018Co-Authors: Rezvan Mobasseri, Seeram Ramakrishna, Masoud Soleimani, Lingling Tian, Hossein NaderimaneshAbstract:Long-term culture, passage and proliferation of human mesenchymal stem cells (hMSCs) cause loss of their stemness properties including self-renewal and multipotency. By optimizing the MSCs environment in vitro, maintaining the stemness state and better controlling the cell fate might be possible. We have recently reported the significant effects of bioactive Tat protein-derived peptide named R-peptide on hMSC adhesion, morphology and proliferation, which has demonstrated R-peptide enhanced MSC early adhesion and proliferation in comparison to other bioactive molecules including RGD peptide, fibronectin and collagen. In this study, R-peptide was used to evaluate stemness properties of MSCs after long-term passaging. R-peptide conjugated poly caprolactone (PCL) Nanofibrous Scaffold and unmodified Nanofibrous Scaffold were used to study the impact of R-peptide modified PCL nanofibers and PCL nanofibers on cell behavior. The results showed early formation of focal adhesion (FA) complex on R-peptide modified Scaffolds at 30min after cell seeding. The rate of cell proliferation was significantly increased due to presence of R-peptide, and the MSCs marker analyses using flow cytometry and immunocytochemistry staining proved the ability of R-peptide to maintain mesenchymal stem cell properties (high proliferation, expression of multipotent markers and differentiation capacity) even after long-term passage culturing. Accordingly, our (The) results concluded that bioactive R-peptide in combination with Nanofibrous Scaffold can mimic the native ECM comprising micro/nano architecture and biochemical molecules in a best way. The designed Scaffold can link extracellular matrix (ECM) to nucleus via formation of FA and organization of cytoskeleton, causing fast and strong attachment of MSCs and allowing integrin-mediated signaling to start.
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Fabrication of a biomimetic ZeinPDA Nanofibrous Scaffold impregnated with BMP-2 peptide conjugated TiO2 nanoparticle for bone tissue engineering.
Journal of Tissue Engineering and Regenerative Medicine, 2017Co-Authors: Sumathy Babitha, Michal Marcin Dykas, Kingshuk Poddar, Meenakshi Annamalai, Jayarama Reddy Venugopal, Thirumalai Venkatesan, Surajit Saha, Seeram Ramakrishna, Purna Sai KorrapatiAbstract:A biomimetic Zein polydopamine (PDA) based nanofiber Scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide (TiO2) nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to TiO2 nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nano topographical stimulation of electrospun Zein PDA nanofibers were examined for its enhanced osteogenic expression of human fetal osteoblast (hFOB) cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization and differentiation could be attributed to its highly interconnected Nanofibrous matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated Nanofibrous Scaffold possess better cell - biomaterial interactions. These promising results demonstrate the potential of the composite Nanofibrous Scaffold as an effective biomaterial substrate for bone regeneration.
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synergistic effect of topography surface chemistry and conductivity of the electrospun Nanofibrous Scaffold on cellular response of pc12 cells
Colloids and Surfaces B: Biointerfaces, 2016Co-Authors: Lingling Tian, Seeram Ramakrishna, Molamma P Prabhakaran, Menglin Chen, Flemming BesenbacherAbstract:Electrospun Nanofibrous nerve implants is a promising therapy for peripheral nerve injury, and its performance can be tailored by chemical cues, topographical features as well as electrical properties. In this paper, a surface modified, electrically conductive, aligned Nanofibrous Scaffold composed of poly (lactic acid) (PLA) and polypyrrole (Ppy), referred to as o-PLAPpy_A, was fabricated for nerve regeneration. The morphology, surface chemistry and hydrophilicity of nanofibers were characterized by Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and water contact angle, respectively. The effects of these nanofibers on neuronal differentiation using PC12 cells were evaluated. A hydrophilic surface was created by Poly-ornithine coating, which was able to provide a better environment for cell attachment, and furthermore aligned fibers were proved to be able to guide PC12 cells grow along the fiber direction and be beneficial for neurite outgrowth. The cellular response of PC12 cells to pulsed electrical stimulation was evaluated by NF 200 and alpha tubulin expression, indicating that electrical stimulation with a voltage of 40mV could enhance the neurite outgrowth. The PC12 cells stimulated with electrical shock showed greater level of neurite outgrowth and smaller cell body size. Moreover, the PC12 cells under electrical stimulation showed better viability. In summary, the o-PLAPpy_A Nanofibrous Scaffold supported the attachment, proliferation and differentiation of PC12 cells in the absence of electrical stimulation, which could be potential candidate for nerve regeneration applications.
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low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic Nanofibrous Scaffold
Journal of Materials Science: Materials in Medicine, 2014Co-Authors: Dillip Kumar Bishi, Jayarama Reddy Venugopal, Soma Guhathakurta, Kotturathu Mammen Cherian, Seeram RamakrishnaAbstract:Liver tissue engineering using polymeric Nanofibrous Scaffold and stem cells holds great promises for treating end-stage liver failures. The aim of this study was to evaluate hepatic trans-differentiation potential of human mesenchymal stem cells (hMSCs) on a biomagnetic electrospun Nanofibrous Scaffold fabricated from a blend of poly-l-lactide (PLLA), collagen and fibrin-rich blood clot, under the influence of a low frequency magnetic field. The Scaffold was characterized for surface properties, biochemical and biomechanical parameters and bio-magnetic behaviour. Cell proliferation assay revealed that the Scaffold was suitable for hMSCs adhesion and proliferation. Hepatic trans-differentiation potential of hMSCs was augmented on Nanofibrous Scaffold in magnetic field exposure group compared to control groups, as evident by strong expression of hepatocyte specific markers, albumin release, urea synthesis and presence of an inducible cytochrome P450 system. Our results conclude that biomagnetic Scaffold of PLLA/collagen/blood clot augments hepatic trans-differentiation of hMSCs under magnetic field influence.
Yufeng Zheng - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterization of electrospun plga gelatin nanofibers as a potential drug delivery system
Colloids and Surfaces B: Biointerfaces, 2011Co-Authors: Z. X. Meng, Yufeng Zheng, Wei Zheng, Huimin Zhou, Xia LouAbstract:Drug (Fenbufen, FBF)-loaded poly(d,l-lactide-co-glycolide) (PLGA) and PLGA/gelatin Nanofibrous Scaffolds were fabricated via electrospinning technique. The influences of gelatin content, fiber arrangement, crosslinking time and pH value of the buffer solution on FBF release behavior of the resulting Nanofibrous Scaffolds were investigated, with the corresponding FBF-loaded PLGA and PLGA/gelatin solvent-cast films as controls. The release rate of FBF was found to be increased with the increment of gelatin content for all the composite samples, and the FBF release rate of aligned Nanofibrous Scaffold was lower than that of randomly oriented Scaffold. Moreover, the crosslinking treatment depressed effectively the burst release of FBF at initial release stage of PLGA/gelatin (9/1) Nanofibrous Scaffold. In addition, the pH value of the buffer solution could change the physical state of the polymer and affect the FBF release rate. © 2010 Elsevier B.V. All rights reserved.
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fabrication characterization and in vitro drug release behavior of electrospun plga chitosan Nanofibrous Scaffold
Materials Chemistry and Physics, 2011Co-Authors: Z. X. Meng, Yufeng Zheng, Wei ZhengAbstract:In this study both aligned and randomly oriented poly(d,l-lactide-co-glycolide) (PLGA)/chitosan Nanofibrous Scaffold have been prepared by electrospinning. The ratio of PLGA to chitosan was adjusted to get smooth nanofiber surface. Morphological characterization using scanning electron microscopy showed that the aligned nanofiber diameter distribution obtained by electrospinning of polymer blend increased with the increase of chitosan content which was similar to that of randomly oriented nanofibers. The release characteristic of model drug fenbufen (FBF) from the FBF-loaded aligned and randomly oriented PLGA and PLGA/chitosan Nanofibrous Scaffolds was investigated. The drug release rate increased with the increase of chitosan content because the addition of chitosan enhanced the hydrophilicity of the PLGA/chitosan composite Scaffold. Moreover, for the aligned PLGA/chitosan Nanofibrous Scaffold the release rate was lower than that of randomly oriented PLGA/chitosan Nanofibrous Scaffold, which indicated that the nanofiber arrangement would influence the release behavior. In addition, crosslinking in glutaraldehyde vapor would decrease the burst release of FBF from FBF-loaded PLGA/chitosan Nanofibrous Scaffold with a PLGA/chitosan ratio less than 9/1, which would be beneficial for drug release.
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Fabrication, characterization and in vitro drug release behavior of electrospun PLGA/chitosan Nanofibrous Scaffold
Materials Chemistry and Physics, 2011Co-Authors: Z. X. Meng, Wei Zheng, Yufeng ZhengAbstract:In this study both aligned and randomly oriented poly(d,l-lactide-co-glycolide) (PLGA)/chitosan Nanofibrous Scaffold have been prepared by electrospinning. The ratio of PLGA to chitosan was adjusted to get smooth nanofiber surface. Morphological characterization using scanning electron microscopy showed that the aligned nanofiber diameter distribution obtained by electrospinning of polymer blend increased with the increase of chitosan content which was similar to that of randomly oriented nanofibers. The release characteristic of model drug fenbufen (FBF) from the FBF-loaded aligned and randomly oriented PLGA and PLGA/chitosan Nanofibrous Scaffolds was investigated. The drug release rate increased with the increase of chitosan content because the addition of chitosan enhanced the hydrophilicity of the PLGA/chitosan composite Scaffold. Moreover, for the aligned PLGA/chitosan Nanofibrous Scaffold the release rate was lower than that of randomly oriented PLGA/chitosan Nanofibrous Scaffold, which indicated that the nanofiber arrangement would influence the release behavior. In addition, crosslinking in glutaraldehyde vapor would decrease the burst release of FBF from FBF-loaded PLGA/chitosan Nanofibrous Scaffold with a PLGA/chitosan ratio less than 9/1, which would be beneficial for drug release.
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electrospinning of plga gelatin randomly oriented and aligned nanofibers as potential Scaffold in tissue engineering
Materials Science and Engineering: C, 2010Co-Authors: Z. X. Meng, Yansong Wang, Yufeng Zheng, Lei Li, W ZhengAbstract:Electrospinning technique can be used to produce the three-dimensional Nanofibrous Scaffold similar to natural extracellular matrix, which satisfies particular requirements of tissue engineering Scaffold. Randomly-oriented and aligned poly(lactic-co-glycolic acid) (PLGA) and PLGA/gelatin biocomposite Scaffolds were successfully produced by electrospinning in the present study. The resulting Nanofibrous Scaffolds exhibited smooth surface and high porous structure. Blending PLGA with gelatin enhanced the hydrophilicity but decreased the average fiber diameter and the mechanical properties of the Scaffolds under the same electrospinning condition. The cell culture results showed that the elongation of the osteoblast on the aligned Nanofibrous Scaffold was parallel to the fiber arrangement and the cell number was similar to that of randomly-oriented Scaffold, indicating that the aligned Nanofibrous Scaffold provide a beneficial approach for the bone regeneration.
Yuqing Niu - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered plla gelatine Nanofibrous Scaffold promoting the phenotypic expression of epithelial and smooth muscle cells for urethral reconstruction
Materials Science and Engineering: C, 2020Co-Authors: Guochang Liu, Zhang Zhao, Huimin Xia, Yuqing NiuAbstract:Abstract The repair and regeneration of tissues using tissue-engineered Scaffolds represent the ultimate goal of regenerative medicine. Despite rapid developments in the field, urethral tissue engineering methods are still insufficient to replicate natural urethral tissue because the bioactivity of existing Scaffolds is inefficient, especially for large tissue defects, which require large tissue-engineered Scaffolds. Here, we describe the efficiency of gelatine-functionalized, tubular Nanofibrous Scaffolds of poly( l -lactic acid) (PLLA) in regulating the phenotypic expression of epithelial cells (ECs) and smooth muscle cells (SMCs) for urethral reconstruction. Flexible PLLA/gelatine tubular Nanofibrous Scaffolds with hierarchical architecture were fabricated by electrospinning. The PLLA/gelatine Nanofibrous Scaffold exhibited enhanced hydrophilicity and significantly promoted the adhesion, oriented elongation, and proliferation of New Zealand rabbit autologous ECs and SMCs simultaneously. Compared with pure PLLA Nanofibrous Scaffold, PLLA/gelatine Nanofibrous Scaffolds upregulated the expression of keratin (AE1/AE3) in ECs and actin (α-SMA) in SMCs as well as the synthesis of elastin. Three months of in vivo Scaffold replacement of New Zealand rabbit urethras indicated that a tubular cellularized PLLA/gelatine Nanofibrous Scaffold maintained urethral patency and facilitated oriented SMC remodeling, lumen epithelialization, and angiogenesis. Our observations showed the synergistic effects of nano-morphology and biochemical clues in the design of biomimetic Scaffolds, which can effectively promote urethral regeneration.
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Tissue-engineered PLLA/gelatine Nanofibrous Scaffold promoting the phenotypic expression of epithelial and smooth muscle cells for urethral reconstruction
Materials science & engineering. C Materials for biological applications, 2020Co-Authors: Guochang Liu, Zhang Zhao, Huimin Xia, Yuqing NiuAbstract:Abstract The repair and regeneration of tissues using tissue-engineered Scaffolds represent the ultimate goal of regenerative medicine. Despite rapid developments in the field, urethral tissue engineering methods are still insufficient to replicate natural urethral tissue because the bioactivity of existing Scaffolds is inefficient, especially for large tissue defects, which require large tissue-engineered Scaffolds. Here, we describe the efficiency of gelatine-functionalized, tubular Nanofibrous Scaffolds of poly( l -lactic acid) (PLLA) in regulating the phenotypic expression of epithelial cells (ECs) and smooth muscle cells (SMCs) for urethral reconstruction. Flexible PLLA/gelatine tubular Nanofibrous Scaffolds with hierarchical architecture were fabricated by electrospinning. The PLLA/gelatine Nanofibrous Scaffold exhibited enhanced hydrophilicity and significantly promoted the adhesion, oriented elongation, and proliferation of New Zealand rabbit autologous ECs and SMCs simultaneously. Compared with pure PLLA Nanofibrous Scaffold, PLLA/gelatine Nanofibrous Scaffolds upregulated the expression of keratin (AE1/AE3) in ECs and actin (α-SMA) in SMCs as well as the synthesis of elastin. Three months of in vivo Scaffold replacement of New Zealand rabbit urethras indicated that a tubular cellularized PLLA/gelatine Nanofibrous Scaffold maintained urethral patency and facilitated oriented SMC remodeling, lumen epithelialization, and angiogenesis. Our observations showed the synergistic effects of nano-morphology and biochemical clues in the design of biomimetic Scaffolds, which can effectively promote urethral regeneration.
Purna Sai Korrapati - One of the best experts on this subject based on the ideXlab platform.
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fabrication of a biomimetic zeinpda Nanofibrous Scaffold impregnated with bmp 2 peptide conjugated tio2 nanoparticle for bone tissue engineering
Journal of Tissue Engineering and Regenerative Medicine, 2018Co-Authors: Sumathy Babitha, Michal Marcin Dykas, Kingshuk Poddar, Meenakshi Annamalai, Jayarama Reddy Venugopal, Thirumalai Venkatesan, Surajit Saha, Seeram Ramakrishna, Purna Sai KorrapatiAbstract:A biomimetic Zein polydopamine based nanofiber Scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to titanium dioxide nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nanotopographical stimulation of electrospun Zein polydopamine nanofiber were examined for its enhanced osteogenic expression of human fetal osteoblast cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization, and differentiation could be attributed to its highly interconnected Nanofibrous matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated Nanofibrous Scaffold possess better cell-biomaterial interactions. These promising results demonstrate the potential of the composite Nanofibrous Scaffold as an effective biomaterial substrate for bone regeneration.
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Fabrication of a biomimetic ZeinPDA Nanofibrous Scaffold impregnated with BMP-2 peptide conjugated TiO2 nanoparticle for bone tissue engineering.
Journal of Tissue Engineering and Regenerative Medicine, 2017Co-Authors: Sumathy Babitha, Michal Marcin Dykas, Kingshuk Poddar, Meenakshi Annamalai, Jayarama Reddy Venugopal, Thirumalai Venkatesan, Surajit Saha, Seeram Ramakrishna, Purna Sai KorrapatiAbstract:A biomimetic Zein polydopamine (PDA) based nanofiber Scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide (TiO2) nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to TiO2 nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nano topographical stimulation of electrospun Zein PDA nanofibers were examined for its enhanced osteogenic expression of human fetal osteoblast (hFOB) cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization and differentiation could be attributed to its highly interconnected Nanofibrous matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated Nanofibrous Scaffold possess better cell - biomaterial interactions. These promising results demonstrate the potential of the composite Nanofibrous Scaffold as an effective biomaterial substrate for bone regeneration.
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Biodegradable zein-polydopamine polymeric Scaffold impregnated with TiO2 nanoparticles for skin tissue engineering.
Biomedical Materials, 2017Co-Authors: Sumathy Babitha, Purna Sai KorrapatiAbstract:Polymers from renewable resources are attractive for various industrial and biomedical applications owing to their compatibility, degradability, ease of use and availability. Rapid progress in the development of nanotechnology has improved the characteristic features of polymers in composite materials by reinforcing the nanosized particulates during fabrication. In this study, we have attempted to incorporate metal oxide nanoparticles into polymeric nanofibers in order to enhance the overall properties of the composite Scaffold. The thermal stability of a TiO2 nanoparticle-impregnated zein-polydopamine-based Nanofibrous Scaffold was investigated, and its potential as a suitable wound dressing material was demonstrated. Further, the influence of nanotopographic structure on improved adhesion, proliferation and migration of cells was ascertained through in vitro assays. The constructive results obtained were well corroborated with the in vivo excisional wound healing experiment. Thus, the competence of the prepared Nanofibrous Scaffold was examined both in vitro and in vivo and demonstrated to be an alternative, cost-effective biomaterial for skin tissue engineering applications.
Narayan C. Mishra - One of the best experts on this subject based on the ideXlab platform.
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surface modification of Nanofibrous polycaprolactone gelatin composite Scaffold by collagen type i grafting for skin tissue engineering
Materials Science and Engineering: C, 2014Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:Abstract In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I composite Nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin Nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the Nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2–1.5 wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I composite Nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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surface modification of Nanofibrous polycaprolactone gelatin composite Scaffold by collagen type i grafting for skin tissue engineering
Materials Science and Engineering: C, 2014Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:Abstract In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I composite Nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin Nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the Nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2–1.5 wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I composite Nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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Surface modification of Nanofibrous polycaprolactone/gelatin composite Scaffold by collagen type I grafting for skin tissue engineering.
Materials Science and Engineering: C, 2013Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I composite Nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin Nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the Nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2-1.5wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I composite Nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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fabrication and characterization of pcl gelatin composite Nanofibrous Scaffold for tissue engineering applications by electrospinning method
Materials Science and Engineering: C, 2013Co-Authors: Sneh Gautam, Amit Kumar Dinda, Narayan C. MishraAbstract:In the present study, composite Nanofibrous tissue engineering-Scaffold consisting of polycaprolactone and gelatin, was fabricated by electrospinning method, using a new cost-effective solvent mixture: chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin. The morphology of the Nanofibrous Scaffold was investigated by using field emission scanning electron microscopy (FE-SEM) which clearly indicates that the morphology of nanofibers was influenced by the weight ratio of PCL to gelatin in the solution. Uniform fibers were produced only when the weight ratio of PCL/gelatin is sufficiently high (10:1). The Scaffold was further characterized by Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) analysis, and X-ray diffraction (XRD). FT-IR and TG analysis indicated some interactions between PCL and gelatin molecules within the Scaffold, while XRD results demonstrated crystalline nature of PCL/gelatin composite Scaffold. Cytotoxicity effect of Scaffold on L929 mouse fibroblast cells was evaluated by MTT assay and cell proliferation on the Scaffold was confirmed by DNA quantification. Positive results of MTT assay and DNA quantification L929 mouse fibroblast cells indicated that the Scaffold made from the combination of natural polymer (gelatin) and synthetic polymer (PCL) may serve as a good candidate for tissue engineering applications.
