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Jacob Gopas - One of the best experts on this subject based on the ideXlab platform.
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Nuphar Lutea extracts exhibit anti viral activity against the measles virus
Molecules, 2020Co-Authors: Hila Winer, Janet Ozer, Daniel Benharroch, Avi Golangoldhirsh, Yonat Shemer, Irit Reichenstein, Brit Eilamfrenkel, Jacob GopasAbstract:Different parts of Nuphar Lutea L. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that Nuphar Lutea extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar Lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.
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6,6'-DihydroxythiobiNupharidine as a poison of human type II topoisomerases.
Bioorganic & medicinal chemistry letters, 2019Co-Authors: Esha D. Dalvie, Jacob Gopas, Avi Golan-goldhirsh, Neil OsheroffAbstract:A number of natural products with medicinal properties increase DNA cleavage mediated by type II topoisomerases. In an effort to identify additional natural compounds that affect the activity of human type II topoisomerases, a blind screen of a library of 341 Mediterranean plant extracts was conducted. Extracts from Nuphar Lutea, the yellow water lily, were identified in this screen. N. Lutea has been used in traditional medicine by a variety of indigenous populations. The active compound in N. Lutea, 6,6'-dihydroxythiobiNupharidine, was found to enhance DNA cleavage mediated by human topoisomerase IIα and IIβ ∼8-fold and ∼3-fold, respectively. Mechanistic studies with topoisomerase IIα indicate that 6,6'-dihydroxythiobiNupharidine is a "covalent poison" that acts by adducting the enzyme outside of the DNA cleavage-ligation active site and requires the N-terminal domain of the protein for its activity. Results suggest that some of the medicinal properties of N. Lutea may result from the interactions between 6,6'-dihydroxythiobiNupharidine and the human type II enzymes.
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anti metastatic effect of semi purified Nuphar Lutea leaf extracts
Journal of Cancer, 2017Co-Authors: Janet Ozer, Avi Golangoldhirsh, Daniel Fishman, Brit Eilam, Jacob GopasAbstract:: Nuphar Lutea L. SM., leaf and rhizome extracts (NUP), contain Nupharidines as active components. Nupharidines belong to the sesquiterpene lactones class of a naturally occurring plant terpenoids. This family of compounds has gained considerable interest for treating infection, inflammation and cancer. NF-κB is a central, downstream regulator of inflammation, cell proliferation and apoptosis. In our previous work we demonstrated strong inhibition of NF-κB activity and induction of apoptosis by NUP. In addition, NUP exhibited anti-inflammatory properties and partial protection from LPS-induced septic shock by modulating ERK pathway and cytokine secretion in macrophages. In the present study, we examined the effect of NUP in a B16 melanoma experimental murine lung metastasis model and its ability to affect the ERK and NF-κB pathways in variety of cell lines. We showed that NUP and cisplatin combined treatment was synergistic and reduced the lung metastatic load. In addition NUP treatment inhibited TNFα-induced IκBα degradation and NF- κB nuclear translocation. We also observed that NUP induced ERK activation. Furthermore, ERK inhibition prevented NF-κB inactivation by NUP. Overall, our work implies that co-administration of NF-κB inhibitors such as NUP, with standard anti-cancer drugs, may act as "sensitizers" for more effective chemotherapy.
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anti inflammatory effect of a Nuphar Lutea partially purified leaf extract in murine models of septic shock
Journal of Ethnopharmacology, 2015Co-Authors: Janet Ozer, Avi Golangoldhirsh, T Levi, Jacob GopasAbstract:ETHNOPHARMACOLOGICAL RELEVANCE: Various plant organs of Nuphar Lutea (L.) SM. (Nymphaeaceae) are used in traditional medicine for the treatment of arthritis, fever, aches, pains and inflammation. The main purpose of this study was to determine the anti-inflammatory effect of Nuphar Lutea leaf extract (NUP) in two septic shock models: (1) Survival of mice challenged with a lethal dose of LPS, determination of pro-inflammatory and anti-inflammatory cytokines in serum, as well as in peritoneal macrophages in cell culture. (2) The effect of NUP in a murine model of fecal-induced peritonitis. MATERIALS AND METHODS: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-κB pathway, modulating cytokine production and ERK phosphorylation. RESULTS: A significant average survival rate (60%) of LPS lethally-challenged mice was achieved by pre-treatment with NUP. In addition, NUP pre-treatment reduced nuclear NF-κB translocation in peritoneal macrophages. The production of pro-inflammatory cytokines, TNF-α, IL-6 and IL-12, in the sera of LPS-treated mice or in the supernatants of peritoneal macrophages stimulated with LPS for 2-6 h was also decreased by NUP. Pre-treatment with NUP caused a significant increase in the anti-inflammatory cytokine IL-10. The NUP pre-treatment reduced and delayed mortality in mice with fecal-induced peritonitis. Our studies also revealed that NUP pre-treatment induced a dose-dependent phosphorylation of ERK in peritoneal macrophages. Since most of the reports about the anti-inflammatory effect of Nuphar Lutea refer to rhizome and root powder and extracts, it is important to clarify the effectiveness of leaf extract as a source for such activity. CONCLUSION: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-κB pathway, modulating cytokine production and ERK phosphorylation.
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leishmania major anti leishmanial activity of Nuphar Lutea extract mediated by the activation of transcription factor nf κb
Experimental Parasitology, 2010Co-Authors: L Ozer, Avi Golangoldhirsh, Joseph Elon, Jacob GopasAbstract:Abstract Here we report the effect of a partially purified alkaloid fraction (NUP) of Nuphar Lutea on nuclear factor kappa B (NF-κB) expression and studied its mechanism of toxicity against Leishmania major in C3H mice peritoneal macrophages. NUP was found to be a mixture of thermo-stable dimeric sesquiterpene thioalkaloids containing mainly thioNupharidines. The anti-leishmanial activity was shown to be mediated through the activation of NF-κB and increased iNOS production. Additionally, the nitric oxide inhibitor, N G -monomethyl- l -arginine (0.5 mM) totally reverted the anti-leishmanial effect of NUP (0.25 and 0.5 μg/ml). NUP was also shown to act as an anti-oxidant, almost completely inhibiting the macrophage respiratory burst activity. However, no elevated lysozyme (EC3.2.1.17) or β-galactosidase (EC3.2.1.23) activities were demonstrated in macrophages treated with NUP. This study suggests, that the activity of NUP is mediated by NF-κB activation and the production of nitric oxide which is dependent on the l -arginine:NO pathway.
Avi Golangoldhirsh - One of the best experts on this subject based on the ideXlab platform.
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Nuphar Lutea extracts exhibit anti viral activity against the measles virus
Molecules, 2020Co-Authors: Hila Winer, Janet Ozer, Daniel Benharroch, Avi Golangoldhirsh, Yonat Shemer, Irit Reichenstein, Brit Eilamfrenkel, Jacob GopasAbstract:Different parts of Nuphar Lutea L. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that Nuphar Lutea extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar Lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.
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anti metastatic effect of semi purified Nuphar Lutea leaf extracts
Journal of Cancer, 2017Co-Authors: Janet Ozer, Avi Golangoldhirsh, Daniel Fishman, Brit Eilam, Jacob GopasAbstract:: Nuphar Lutea L. SM., leaf and rhizome extracts (NUP), contain Nupharidines as active components. Nupharidines belong to the sesquiterpene lactones class of a naturally occurring plant terpenoids. This family of compounds has gained considerable interest for treating infection, inflammation and cancer. NF-κB is a central, downstream regulator of inflammation, cell proliferation and apoptosis. In our previous work we demonstrated strong inhibition of NF-κB activity and induction of apoptosis by NUP. In addition, NUP exhibited anti-inflammatory properties and partial protection from LPS-induced septic shock by modulating ERK pathway and cytokine secretion in macrophages. In the present study, we examined the effect of NUP in a B16 melanoma experimental murine lung metastasis model and its ability to affect the ERK and NF-κB pathways in variety of cell lines. We showed that NUP and cisplatin combined treatment was synergistic and reduced the lung metastatic load. In addition NUP treatment inhibited TNFα-induced IκBα degradation and NF- κB nuclear translocation. We also observed that NUP induced ERK activation. Furthermore, ERK inhibition prevented NF-κB inactivation by NUP. Overall, our work implies that co-administration of NF-κB inhibitors such as NUP, with standard anti-cancer drugs, may act as "sensitizers" for more effective chemotherapy.
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anti inflammatory effect of a Nuphar Lutea partially purified leaf extract in murine models of septic shock
Journal of Ethnopharmacology, 2015Co-Authors: Janet Ozer, Avi Golangoldhirsh, T Levi, Jacob GopasAbstract:ETHNOPHARMACOLOGICAL RELEVANCE: Various plant organs of Nuphar Lutea (L.) SM. (Nymphaeaceae) are used in traditional medicine for the treatment of arthritis, fever, aches, pains and inflammation. The main purpose of this study was to determine the anti-inflammatory effect of Nuphar Lutea leaf extract (NUP) in two septic shock models: (1) Survival of mice challenged with a lethal dose of LPS, determination of pro-inflammatory and anti-inflammatory cytokines in serum, as well as in peritoneal macrophages in cell culture. (2) The effect of NUP in a murine model of fecal-induced peritonitis. MATERIALS AND METHODS: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-κB pathway, modulating cytokine production and ERK phosphorylation. RESULTS: A significant average survival rate (60%) of LPS lethally-challenged mice was achieved by pre-treatment with NUP. In addition, NUP pre-treatment reduced nuclear NF-κB translocation in peritoneal macrophages. The production of pro-inflammatory cytokines, TNF-α, IL-6 and IL-12, in the sera of LPS-treated mice or in the supernatants of peritoneal macrophages stimulated with LPS for 2-6 h was also decreased by NUP. Pre-treatment with NUP caused a significant increase in the anti-inflammatory cytokine IL-10. The NUP pre-treatment reduced and delayed mortality in mice with fecal-induced peritonitis. Our studies also revealed that NUP pre-treatment induced a dose-dependent phosphorylation of ERK in peritoneal macrophages. Since most of the reports about the anti-inflammatory effect of Nuphar Lutea refer to rhizome and root powder and extracts, it is important to clarify the effectiveness of leaf extract as a source for such activity. CONCLUSION: NUP pre-treatment partially protected mice in two models of acute septic shock. We concluded that NUP is anti-inflammatory by inhibiting the NF-κB pathway, modulating cytokine production and ERK phosphorylation.
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leishmania major anti leishmanial activity of Nuphar Lutea extract mediated by the activation of transcription factor nf κb
Experimental Parasitology, 2010Co-Authors: L Ozer, Avi Golangoldhirsh, Joseph Elon, Jacob GopasAbstract:Abstract Here we report the effect of a partially purified alkaloid fraction (NUP) of Nuphar Lutea on nuclear factor kappa B (NF-κB) expression and studied its mechanism of toxicity against Leishmania major in C3H mice peritoneal macrophages. NUP was found to be a mixture of thermo-stable dimeric sesquiterpene thioalkaloids containing mainly thioNupharidines. The anti-leishmanial activity was shown to be mediated through the activation of NF-κB and increased iNOS production. Additionally, the nitric oxide inhibitor, N G -monomethyl- l -arginine (0.5 mM) totally reverted the anti-leishmanial effect of NUP (0.25 and 0.5 μg/ml). NUP was also shown to act as an anti-oxidant, almost completely inhibiting the macrophage respiratory burst activity. However, no elevated lysozyme (EC3.2.1.17) or β-galactosidase (EC3.2.1.23) activities were demonstrated in macrophages treated with NUP. This study suggests, that the activity of NUP is mediated by NF-κB activation and the production of nitric oxide which is dependent on the l -arginine:NO pathway.
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Nuphar Lutea thioalkaloids inhibit the nuclear factor kappab pathway potentiate apoptosis and are synergistic with cisplatin and etoposide
Cancer Biology & Therapy, 2009Co-Authors: Janet Ozer, Nadav Eisner, Elena Ostrozhenkova, Daniel Benharroch, Avi Golangoldhirsh, Adelbert Bacher, Wolfgang Eisenreich, Jacob GopasAbstract:We screened thirty-four methanolic plant extracts for inhibition of the constitutive nuclear factor κB (NF-κB) activity by a NF-κB-luciferase reporter gene assay. Strong inhibition of NF-κB activity was found in extracts of leaf and rhizome from Nuphar Lutea L. SM. (Nuphar). The inhibitory action was narrowed down to a mixture of thioNupharidines and/or thionuphlutidines that were identified in chromatography fractions by one- and two-dimensional NMR analysis. Dimeric sesquiterpene thioalkaloids were identified as the major components of the mixture. The Nuphar alkaloids mixture (NUP) showed a dose dependent inhibition of NF-κB activity in a luciferase reporter gene assay as well as reduction of nuclear NF-κB subunits expression as tested by western blots and immunohistochemistry. Decreased DNA binding was demonstrated in electro mobility shift assays. NUP inhibited both inducible and constitutive NF-κB activation and affected the canonical and alternative pathways. Suppression of NF-κB was not cell type ...
Jari Kouki - One of the best experts on this subject based on the ideXlab platform.
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the effect of the water lily beetle galerucella nymphaeae on leaf production and leaf longevity of the yellow water lily Nuphar Lutea
Freshwater Biology, 1991Co-Authors: Jari KoukiAbstract:SUMMARY. 1. The effect of the water-lily beetle, Galerucella (=Pyrrhalta) nymphaeae (L.), (Coleoptera, Chrysomelidae), on the leaf production and leaf longevity of the yellow water-lily, Nuphar Lutea (=N. luteum) (L.) Sibth. & Sm., was studied experimentally in southern Finland during one growth season. 2. Twelve cages, each containing a single rosette of the water-lily, were established in the lake during May. Two treatments were randomly applied to cages: (i) control (herbivory not allowed), and (ii) fifty beetles added at the beginning of the season. Leaves were marked individually and the production of new leaves was followed until October. 3. In both treatments the production of floating leaves continued until the end of August but declined towards the end of summer. Herbivory did not affect the number of leaves produced, i.e. the plants did not compensate for the loss of leaves. 4. Leaf longevity was reduced dramatically (up to —60%) when herbivores were present. Leaves that emerged during the most intense period of larval growth had a life-span one-third of that of the control group.
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Tracking spatially variable resources : an experimental study on the oviposition of the water-lily beetle
Oikos, 1991Co-Authors: Jari KoukiAbstract:The water-lilly leaf beetle Galerucella nymphaeae is a specialist herbivore of the floating-leaved Nuphar Lutea. Floating leaves occur in patches with different densities. I studied experimentally the oviposition responses to spatially varying patch characteristics. In the manipulations I changed density of leaves, quality (as measured by the previous herbivory) of leaves and number of eggs laid on a leaf
Benjamin Nagengast - One of the best experts on this subject based on the ideXlab platform.
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Translocation of Nuphar Lutea (L.) Sibth. & Sm. from the A2 road near Nowy Tomyśl (Poland) into alternative sites
Biodiversity: Research and Conservation, 2011Co-Authors: Anna Borysiak, Janina Borysiak, Tomasz Joniak, Benjamin NagengastAbstract:A translocation of Nuphar Lutea (L.) Sibth. & Sm. was made from its primary location in PrπdUwka, situated in the path of the construction site of the A2 motorway, into replacement biotopes. All rhizomes were taken out of the water and used to prepare cuttings. These were divided into three approximately homogeneous groups. Each group was introduced into different replacement water bodies with environmental conditions that resembled those of the biotope in the PrπdUwka site. Two of the water bodies were fish ponds. A third was a so called Cecological water bodyi (settling pond, settling basin) that collected fall effluents delivered from the surface of motorway. The results of the examination of the physical-chemical features of water and bottom sediments from the PrπdUwka peat pit, two fish ponds and four settling basins are presented in the article. Biological and autecological features of the species Nuphar Lutea , which are significant from the aspect of the growth of the seedlings within the alternative sites, have been discussed. A year after translocation it was observed that in the case of fish ponds, 83% and 75% of the introduced plants survived, while in the settling basin only 50%. A conservation project was undertaken by the investor in order to compensate for the negative effects of the A2 motorway within the area of the European Ecological Natura 2000 Network.
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translocation of Nuphar Lutea l sibth sm from the a2 road near nowy tomyśl poland into alternative sites
Biodiversity: Research and Conservation, 2011Co-Authors: Anna Borysiak, Janina Borysiak, Tomasz Joniak, Benjamin NagengastAbstract:A translocation of Nuphar Lutea (L.) Sibth. & Sm. was made from its primary location in PrπdUwka, situated in the path of the construction site of the A2 motorway, into replacement biotopes. All rhizomes were taken out of the water and used to prepare cuttings. These were divided into three approximately homogeneous groups. Each group was introduced into different replacement water bodies with environmental conditions that resembled those of the biotope in the PrπdUwka site. Two of the water bodies were fish ponds. A third was a so called Cecological water bodyi (settling pond, settling basin) that collected fall effluents delivered from the surface of motorway. The results of the examination of the physical-chemical features of water and bottom sediments from the PrπdUwka peat pit, two fish ponds and four settling basins are presented in the article. Biological and autecological features of the species Nuphar Lutea , which are significant from the aspect of the growth of the seedlings within the alternative sites, have been discussed. A year after translocation it was observed that in the case of fish ponds, 83% and 75% of the introduced plants survived, while in the settling basin only 50%. A conservation project was undertaken by the investor in order to compensate for the negative effects of the A2 motorway within the area of the European Ecological Natura 2000 Network.
Charles P Henriot - One of the best experts on this subject based on the ideXlab platform.
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relationships between key functional traits of the waterlily Nuphar Lutea and wetland nutrient content
PeerJ, 2019Co-Authors: Charles P Henriot, Quentin Cuenot, Lisehelene Levrey, Christophe Loup, Landry Chiarello, Helene Masclaux, Gudrun BornetteAbstract:: Little attention has been paid to how aquatic habitat characteristics affect the traits of plant species. Nuphar Lutea (L.) Sm. is a keystone species distributed across temperate regions of Europe, northwest Africa and western Asia. Its apparently low phenotypic variability compared to other aquatic plants led us to test whether the species exhibited significant phenotypic variability and whether trait values correlated to environmental parameters. The hypotheses were that (1) the environmental variation within our set of wetlands (both water and sediment characteristics) led to significant variation among four sets of traits related respectively to growth, reproduction, defence and storage and (2) that nutrient limitation (nitrogen and especially phosphorus) should affect plant traits towards a higher investment in storage and defence and a lower investment in growth and reproduction, thereby negatively affecting the success of N. Lutea. To test these hypotheses, 11 populations of N. Lutea were sampled in wetlands differing in physicochemical characteristics and spread along three rivers. A total of 15 traits, grouped into four sets (growth, reproduction, storage and defence), were measured during the growing season. Most N. Lutea traits were related to the environmental characteristics of wetlands. The growth and reproduction traits were mostly positively related to habitat resource conditions, whereas the defence traits were positively correlated with both ammonium concentration and temperature, outlining possible anoxic stress (habitat adversity). Nitrogen or phosphorus limitation led to the variation of only a few traits: the rhizome starch content was higher in phosphorus-limited wetlands, while the rhizome length and volume, and the number of flowers were higher in nitrogen-limited wetlands.
