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R Cepedapalacios - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Abstract Two studies were carried out in order to test the effects of neem tree extracts ( Azadirachta indica A. Juss) on sheep bot fly larvae ( Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro . In a second study, the effect of oral administration with neem seed meal (0, 100 y 200 mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis -infected sheep. Results in Experiment 1 showed a significant ( P
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessandra Scala, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, A Scala, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the summer season. Sarda x Lacaune ewes (n=186), divided into three ram-families were used in the study. Systemic and local IgG responses were measured by ELISA tests using second stage larval crude extracts (L2CE) and L2 (L2SGC) and L3 (L3SGC) salivary gland contents as coating antigens. The number of larval instars, larval length of L1, L2 and L3 larvae, and larval weight of L2 and L3 larvae were individually recorded after ewe necropsy. Negative correlations among larval establishment and/or larval development on the one hand and intensity of local or systemic IgG responses on the other hand were found in two out of three studied ram-families.
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factors affecting the larvipositional activity of oestrus ovis gravid females diptera Oestridae
Veterinary Parasitology, 2000Co-Authors: R Cepedapalacios, Philip J SchollAbstract:Abstract Observations of fly strikes or larvipositions ( n =68 in 21 days of observation) were carried out in a herd of goats during the spring in Baja California Sur, Mexico in order to identify the climatic conditions favoring larviposition activity of gravid Oestrus ovis L. flies, as well as to investigate whether a mixture of some potentially useful compounds was involved in this behavior. Hand-caught, tethered flies ( n =43) were either exposed or unexposed to a combination of carbon dioxide, humidity, 1-octen-3-ol, butyric, propionic, acetic acid and acetone released from movable sheep and goat dummies under open field and cage conditions. Fly strikes occurred at temperatures greater than 20°C, but mainly between 25 and 28°C and from 116 to 838 W m −2 of solar irradiance. Few or no strikes were seen under moderate or strong wind, but did occur in a wide range of relative humidity. The chemicals applied did not improve the capacity of animal dummies to induce the flies to larviposit, but very irregular behavior was observed. Fourteen larvipositions were made on the dummies lacking chemical stimuli, so visual ability and movement by the dummies was very important in stimulation of the flies. Temperature appeared to be the main factor determining fly activity, but wind and solar irradiance also played important roles. Characteristics of O. ovis larviposition are discussed.
Ph Dorchies - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Abstract Two studies were carried out in order to test the effects of neem tree extracts ( Azadirachta indica A. Juss) on sheep bot fly larvae ( Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro . In a second study, the effect of oral administration with neem seed meal (0, 100 y 200 mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis -infected sheep. Results in Experiment 1 showed a significant ( P
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessandra Scala, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, A Scala, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the summer season. Sarda x Lacaune ewes (n=186), divided into three ram-families were used in the study. Systemic and local IgG responses were measured by ELISA tests using second stage larval crude extracts (L2CE) and L2 (L2SGC) and L3 (L3SGC) salivary gland contents as coating antigens. The number of larval instars, larval length of L1, L2 and L3 larvae, and larval weight of L2 and L3 larvae were individually recorded after ewe necropsy. Negative correlations among larval establishment and/or larval development on the one hand and intensity of local or systemic IgG responses on the other hand were found in two out of three studied ram-families.
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oestrus ovis in sheep relative third instar populations risks of infection and parasitic control
Veterinary Research, 2001Co-Authors: Guillaume Tabouret, Ph Jacquiet, Philip J Scholl, Ph DorchiesAbstract:Oestrus ovis (L.) (Diptera: Oestridae), the nasal bot fly, has a relatively short free-living life cycle outside of the host, and therefore it is necessary to know when the parasitic period occurs in order to prevent the clinical signs and economic losses caused by this parasite. The length of this parasitic portion of the life cycle is quite variable: a few weeks to several months depending on the season and climatic conditions. Surveys of Oestrus ovis larval populations in sheep show different results on the number of generations according to the local climate. Mean monthly larval profiles of L1 and L3 burdens of sheep from West African Sahelian countries, Mediterranean countries (Morocco, Tunisia and Sicily) and Southwest France were compared. Valuable information on the suspected exten- sion of the fly season is obtained showing the period of infection in each area. This knowledge will be a valuable tool to help in choosing the right treatment at the right period.
Domenico Otranto - One of the best experts on this subject based on the ideXlab platform.
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the mitochondrial genome of the common cattle grub hypoderma lineatum
Medical and Veterinary Entomology, 2010Co-Authors: Stefania Weigl, Donato Traversa, Gabriella Testini, Filipe Dantastorres, Antonio Parisi, Douglas D. Colwell, Domenico OtrantoAbstract:The mitochondrial DNA of the cattle grub Hypoderma lineatum (de Villers) (Diptera: Oestridae) was completely sequenced. The entire molecule was 16 354 bp long and presented a heavy bias towards A + T, which accounted for 77.8% of the whole genome. Hypoderma lineatum genes were organized in the same order and orientation as in the mitochondrial genomes available for other species belonging to the Oestroidea superfamily and compared in this study [Chrysomya putoria (Wiedemann), Cochliomyia hominivorax (Coquerel), Lucilia sericata (Meigen) and Dermatobia hominis (L.)], except for the occurrence of a 102-bp non-coding region partially present in other species. The complete sequence of H. lineatum will represent a useful dataset to evaluate the evolutionary pattern of mtDNA within Oestroidea by using molecular information in diagnostic, taxonomic and evolutionary studies.
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Analysis of somatic and salivary gland antigens of third stage larvae of Rhinoestrus spp. (Diptera, Oestridae).
Experimental Parasitology, 2009Co-Authors: Piermarino Milillo, Donato Traversa, Gabriella Elia, Domenico OtrantoAbstract:Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays performed using pharyngeal swabs. The present study was carried out to characterize larval somatic proteins and salivary glands of Rhinoestrus spp. in a preliminary assessment towards the immunodiagnosis of equine rhinoestrosis. Out of the 212 necropsied horses 13 were positive for the presence of Rhinoestrus spp. larvae. The analysis of the sera from the infected animals by Western blotting assay showed the presence of a specific host humoral immune response against Rhinoestrus spp. larvae and proved that the salivary glands are the major immunogens in horse nasal botflies.
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oestrus ovis causing human ocular myiasis from countryside to town centre
Clinical and Experimental Ophthalmology, 2009Co-Authors: Domenico Otranto, Marilina Santantonio, Cinzia Cantacessi, Giovanni RizzoAbstract:Oestrosis caused by larvae of the sheep nasal botfly, Oestrus ovis (Diptera, Oestridae), is a zoonotic nasal myiasis affecting small ruminants (mainly sheep and goats) across the world, particularly in African and European Mediterranean countries. Reports of human oestrosis generally refer to people who have been closely associated with sheep and goat husbandry such as shepherds and farmers. Different clinical forms of human oestrosis are known, including external ophthalmitis, pharyngeal or laryngeal myiasis, nasal or auricular forms, with the possibility of combined clinical presentations. Reports of human infection by O. ovis have been increasing over the last few years and, recently, the first case of human oestrosis has been detected in a farmer living in the Canary Islands. Here we report the first confirmed case of human ocular myiasis by O. ovis, which has occurred in an urban environment.
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efficacy of moxidectin injectable and pour on formulations in a pilot control program against bovine hypodermosis in southern italy
Preventive Veterinary Medicine, 2005Co-Authors: Domenico Otranto, Donato Traversa, Alessandro Agostini, Piermarino Milillo, Gioia CapelliAbstract:Bovine hypodermosis is a myiasis caused by Hypoderma bovis and Hypoderma lineatum (Diptera, Oestridae) larvae, which has a severe economic impact on the livestock industry. Though myiasis is widespread throughout Italy, no nationwide eradication program has ever been planned, unlike in other European Countries. With a view to setting up a national control program, a pilot study was carried out in Southern Italy on 9939 cattle bred in an area with a high prevalence of cattle hypodermosis, using moxidectin 0.5% pour-on (Cydectin®, Fort Dodge) and 1% injectable (Cydectin®, Fort Dodge) formulations. At the recommended dosage, moxidectin displayed efficacy levels of 99.9% in the pour-on and 100% in the injectable formulation, whereas the microdose (1 mg per head regardless of body weight) was less effective (65.7%). This trial contributed to a significant reduction in infestation rates in the study area and represented the first step through which a national program for eradicating warble fly infestation in Italy.
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short communication efficacy of moxidectin injectable and pour on formulations in a pilot control program against bovine hypodermosis in southern italy
2005Co-Authors: Domenico Otranto, Donato Traversa, Alessandro Agostini, Piermarino Milillo, Gioia CapelliAbstract:Bovine hypodermosis is a myiasis caused by Hypoderma bovis and Hypoderma lineatum (Diptera, Oestridae) larvae, which has a severe economic impact on the livestock industry. Though myiasis is widespread throughout Italy, no nationwide eradication program has ever been planned, unlike in other European Countries. With a view to setting up a national control program, a pilot study was carried out in Southern Italy on 9939 cattle bred in an area with a high prevalence of cattle hypodermosis, using moxidectin 0.5% pour-on (Cydectin 1 , Fort Dodge) and 1% injectable (Cydectin 1 , Fort Dodge) formulations. At the recommended dosage, moxidectin displayed efficacy levels of 99.9% in the pour-on and 100% in the injectable formulation, whereas the microdose (1 mg per head regardless of body weight) was less effective (65.7%). This trial contributed to a significant reduction in infestation rates in the study area and represented the first step through which a national program for eradicating warble fly infestation in Italy. # 2005 Elsevier B.V. All rights reserved.
Jamie R. Stevens - One of the best experts on this subject based on the ideXlab platform.
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species identification of hypoderma affecting domestic and wild ruminants by morphological and molecular characterization
Medical and Veterinary Entomology, 2003Co-Authors: Domenico Otranto, Donato Traversa, Douglas D. Colwell, Jamie R. StevensAbstract:. Cuticular structures and the sequence of the cytochrome oxidase I gene were compared for Hypoderma bovis (Linnaeus), Hypoderma lineatum (De Villers), Hypoderma actaeon Brauer, Hypoderma diana Brauer and Hypoderma tarandi (Linnaeus) (Diptera, Oestridae). Third-stage larvae of each species were examined by scanning electron microscopy revealing differences among species in the pattern and morphology of spines on the cephalic and thoracic segments, by spine patterns on the tenth abdominal segment, and by morphology of the spiracular plates. The morphological approach was supported by the molecular characterization of the most variable region of the cytochrome oxidase I (COI) gene of these species, which was amplified by polymerase chain reaction and analysed. Amplicons were digested with the unique restriction enzyme, BfaI, providing diagnostic profiles able to simultaneously differentiate all Hypoderma species examined. These findings confirm the utility of morphological characters for differentiating the most common Hypoderma larvae and reconfirm the power of the COI gene for studying insect identification and systematics.
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the evolution of myiasis in blowflies calliphoridae
International Journal for Parasitology, 2003Co-Authors: Jamie R. StevensAbstract:Blowflies (Calliphoridae) are characterised by the ability of their larvae to develop in animal flesh. Where the host is a living vertebrate, such parasitism by dipterous larvae is known as myiasis. However, the evolutionary origins of the myiasis habit in the Calliphoridae, a family which includes the blowflies and screwworm flies, remain unclear. Species associated with an ectoparasitic lifestyle can be divided generally into three groups based on their larval feeding habits: saprophagy, facultative ectoparasitism, and obligate parasitism, and it has been proposed that this functional division may reflect the progressive evolution of parasitism in the Calliphoridae. In order to evaluate this hypothesis, phylogenetic analysis of 32 blowfly species displaying a range of forms of ectoparasitism from key subfamilies, i.e. Calliphorinae, Luciliinae, Chrysomyinae, Auchmeromyiinae and Polleniinae, was undertaken using likelihood and parsimony methods. Phylogenies were constructed from the nuclear 28S large subunit ribosomal RNA gene (28S rRNA), sequenced from each of the 32 calliphorid species, together with suitable outgroup taxa, and mitochondrial cytochrome oxidase subunit I and II (COI+II) sequences, derived primarily from published data. Phylogenies derived from each of the two markers (28S rRNA, COI+II) were largely (though not completely) congruent, as determined by incongruence-length difference and Kishino-Hasegawa tests. However, the phylogenetic relationships of blowfly subfamilies based on molecular data did not concur with the pattern of relationships defined by previous morphological analysis; significantly, molecular analysis supported the monophyly of blowflies (Calliphoridae), distinct from the bot and warble flies (Oestridae). Comparative analysis of the myiasis habit based primarily on the 28S rRNA phylogeny indicated that obligate parasitism, and the ability to initiate myiasis in higher vertebrates, has multiple independent origins across myiasis-causing flies (Calliphoridae and Oestridae) and in at least three subfamilies of blowfly (Calliphoridae). Finally, the general association of various blowfly genera and subfamily clades with particular continental and geographical regions suggests that these groups probably came into existence in the Late Cretaceous period, following the break-up of Gondwana.
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molecular characterization of the mitochondrial cytochrome oxidase i gene of Oestridae species causing obligate myiasis
Medical and Veterinary Entomology, 2003Co-Authors: Domenico Otranto, Elvira Tarsitano, Donato Traversa, B Guida, P Fiorente, Jamie R. StevensAbstract:. A 688-bp region of the mitochondrial cytochrome oxidase I gene was sequenced from larvae of 18 species of Oestridae causing obligate myiasis. Larvae belonged to the four Oestridae subfamilies (Cuterebrinae, Gasterophilinae, Hypodermatinae and Oestrinae), which are commonly found throughout the world. Analysis of both nucleotide and amino acid data was performed. Nucleotide sequences included 385 conserved sites and 303 variable sites; mean nucleotide variation between all species was 18.1% and variation within each subfamily ranged from 5.3% to 13.34%. Intraspecific pairwise divergences ranged from 0.14% to 1.59%, and interspecific variation ranged from 0.7% to 27%. Of the 229 amino acids, 76 were variable (60 of which were phylogenetically informative), with some highly conserved residues identified within each subfamily. Phylogenetic analysis showed a strong divergence among the four subfamilies, concordant with classical taxonomy based on morphological and biological features. This study provides the first molecular data set for myiasis-causing Oestridae species, providing an essential database for the molecular identification of these parasites and the assessment of phylogenetic relationships within family Oestridae.
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molecular differentiation of hypoderma bovis and hypoderma lineatum diptera Oestridae by polymerase chain reaction restriction fragment length polymorphism pcr rflp
Veterinary Parasitology, 2003Co-Authors: Domenico Otranto, Elvira Tarsitano, Donato Traversa, Jamie R. StevensAbstract:The most variable region of the cytochrome oxidase I (COI) gene of Hypoderma bovis1 and Hypoderma lineatum2 (Diptera, Oestridae) was amplified by PCR and the amplicons were sequenced and analysed. PCR products were digested with three restriction enzymes, namely BfaI, HinfI and TaqI, providing informative profiles. H. bovis and H. lineatum sequences revealed an inter-specific variation rate of 8.5%, and an intra-specific variation rate of 0.87 and 0.29%, respectively. The results showed that the COI gene region examined was useful for the differentiation of H. bovis and H. lineatum and that a PCR-RFLP assay is a practical tool for their identification, offering additional diagnostic and epidemiological instruments for the study of cattle grub infestation.
C E Angulovaladez - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).
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in vitro and in vivo effects of neem tree azadirachta indica a juss products on larvae of the sheep nose bot fly oestrus ovis l diptera Oestridae
Veterinary Parasitology, 2014Co-Authors: R Cepedapalacios, Ph Dorchies, Felipe Ascencio, R Servin, J M Ramirezorduna, C E AngulovaladezAbstract:Abstract Two studies were carried out in order to test the effects of neem tree extracts ( Azadirachta indica A. Juss) on sheep bot fly larvae ( Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro . In a second study, the effect of oral administration with neem seed meal (0, 100 y 200 mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis -infected sheep. Results in Experiment 1 showed a significant ( P
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessandra Scala, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the
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specific igg antibody responses in oestrus ovis l diptera Oestridae infected sheep associations with intensity of infection and larval development
Veterinary Parasitology, 2008Co-Authors: C E Angulovaladez, Jean Paul Bergeaud, Alessanda Carta, Getachew Terefe, R Cepedapalacios, Christelle Grisez, Frédéric Prévot, Felipe Ascencio, A Scala, Ph DorchiesAbstract:Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the summer season. Sarda x Lacaune ewes (n=186), divided into three ram-families were used in the study. Systemic and local IgG responses were measured by ELISA tests using second stage larval crude extracts (L2CE) and L2 (L2SGC) and L3 (L3SGC) salivary gland contents as coating antigens. The number of larval instars, larval length of L1, L2 and L3 larvae, and larval weight of L2 and L3 larvae were individually recorded after ewe necropsy. Negative correlations among larval establishment and/or larval development on the one hand and intensity of local or systemic IgG responses on the other hand were found in two out of three studied ram-families.
