The Experts below are selected from a list of 1992 Experts worldwide ranked by ideXlab platform
Rui L. Reis - One of the best experts on this subject based on the ideXlab platform.
-
Current advances in solid free-form techniques for Osteochondral Tissue Engineering
Bio-Design and Manufacturing, 2018Co-Authors: João Bebiano Costa, Rui L. Reis, Joana Silva-correia, Joaquim M. OliveiraAbstract:Osteochondral (OC) lesions are characterized by defects in two different zones, the cartilage region and subchondral bone region. These lesions are frequently associated with mechanical instability, as well as osteoarthritic degenerative changes in the knee. The lack of spontaneous healing and the drawbacks of the current treatments have increased the attention from the scientific community to this issue. Different Tissue Engineering approaches have been attempted using different polymers and different scaffolds’ processing. However, the current conventional techniques do not allow the full control over scaffold fabrication, and in this type of approaches, the tuning ability is the key to success in Tissue regeneration. In this sense, the researchers have placed their efforts in the development of solid free-form (SFF) techniques. These techniques allow tuning different properties at the micro–macro scale, creating scaffolds with appropriate features for OC Tissue Engineering. In this review, it is discussed the current SFF techniques used in OC Tissue Engineering and presented their promising results and current challenges.
-
Bioceramics for Osteochondral Tissue Engineering and Regeneration.
Advances in experimental medicine and biology, 2018Co-Authors: S Pina, Rita Rebelo, Vitor M. Correlo, J. Miguel Oliveira, Rui L. ReisAbstract:Considerable advances in Tissue Engineering and regeneration have been accomplished over the last decade. Bioceramics have been developed to repair, reconstruct, and substitute diseased parts of the body and to promote Tissue healing as an alternative to metallic implants. Applications embrace hip, knee, and ligament repair and replacement, maxillofacial reconstruction and augmentation, spinal fusion, bone filler, and repair of periodontal diseases. Bioceramics are well-known for their superior wear resistance, high stiffness, resistance to oxidation, and low coefficient of friction. These specially designed biomaterials are grouped in natural bioceramics (e.g., coral-derived apatites), and synthetic bioceramics, namely bioinert ceramics (e.g., alumina and zirconia), bioactive glasses and glass ceramics, and bioresorbable calcium phosphates-based materials. Physicochemical, mechanical, and biological properties, as well as bioceramics applications in diverse fields of Tissue Engineering are presented herein. Ongoing clinical trials using bioceramics in Osteochondral Tissue are also considered. Based on the stringent requirements for clinical applications, prospects for the development of advanced functional bioceramics for Tissue Engineering are highlighted for the future.
-
layered scaffolds for Osteochondral Tissue Engineering
Advances in Experimental Medicine and Biology, 2018Co-Authors: D R Pereira, Rui L. Reis, Miguel J OliveiraAbstract:Despite huge efforts, Tissue engineers and orthopedic surgeons still face a great challenge to functionally repair Osteochondral (OC) defects. Nevertheless, over the past decade great progress has been made to find suitables strategies towards OC regeneration. In the clinics, some Osteochondral Tissue Engineering (OCTE) strategies have already been applied although with some incongruous outcomes as OC Tissue is complex in its architecture and function. In this chapter, we have summarized current OCTE strategies that are focused on hierarchical scaffold design, mainly layered scaffolds. Most suitable candidates towards functional regeneration of OC Tissues are envisaged from monophasic to layered scaffolds. Herein is documented a variety of strategies with their intrinsic properties for further application as bare scaffolds or in combination with biologics. Both in vitro and in vivo approaches have been thoroughly studied aiming at functional OC regeneration. The most noteworthy studies in OC regeneration developed within the past 5 years are herein documented as well as some current clinical trials.
-
nanoparticles based systems for Osteochondral Tissue Engineering
Advances in Experimental Medicine and Biology, 2018Co-Authors: Isabel Oliveira, Miguel J Oliveira, Silvia Vieira, Rui L. ReisAbstract:Osteochondral lesions represent one of the major causes of disabilities in the world. These defects are due to degenerative or inflammatory arthritis, but both affect the articular cartilage and the underlying subchondral bone. Defects from trauma or degenerative pathology frequently cause severe pain, joint deformity, and loss of joint motion. Osteochondral defects are a significant challenge in orthopedic surgery, due to the cartilage complexity and unique structure, as well as its exposure to high pressure and motion. Although there are treatments routinely performed in the clinical practice, they present several limitations. Tissue Engineering can be a suitable alternative for Osteochondral defects since bone and cartilage Engineering had experienced a notable advance over the years. Allied with nanotechnology, Osteochondral Tissue Engineering (OCTE) can be leveled up, being possible to create advanced structures similar to the OC Tissue. In this chapter, the current strategies using nanoparticles-based systems are overviewed. The results of the studies herein considered confirm that advanced nanomaterials will undoubtedly play a crucial role in the design of strategies for treatment of Osteochondral defects in the near future.
-
the use of electrospinning technique on Osteochondral Tissue Engineering
Advances in Experimental Medicine and Biology, 2018Co-Authors: Marta R Casanova, Rui L. Reis, Albino Martins, Nuno M NevesAbstract:Electrospinning, an electrostatic fiber fabrication technique, has attracted significant interest in recent years due to its versatility and ability to produce highly tunable nanofibrous meshes. These nanofibrous meshes have been investigated as promising Tissue Engineering scaffolds since they mimic the scale and morphology of the native extracellular matrix. The sub-micron diameter of fibers produced by this process presents various advantages like the high surface area to volume ratio, tunable porosity, and the ability to manipulate the nanofiber composition in order to get desired properties and functionality. Electrospun fibers can be oriented or arranged randomly, giving control over both mechanical properties and the biological response to the fibrous scaffold. Moreover, bioactive molecules can be integrated with the electrospun nanofibrous scaffolds in order to improve the cellular response. This chapter presents an overview of the developments on electrospun polymer nanofibers including processing, structure, and their applications in the field of Osteochondral Tissue Engineering.
Jianxin Wang - One of the best experts on this subject based on the ideXlab platform.
-
biomimetic bacterial cellulose enhanced double network hydrogel with excellent mechanical properties applied for the Osteochondral defect repair
ACS Biomaterials Science & Engineering, 2018Co-Authors: Xiangbo Zhu, Taijun Chen, Bo Feng, Jie Weng, Ke Duan, Jianxin WangAbstract:Although hydrogels based on biopolymers show many advantages, their low mechanical properties limit their applications in Osteochondral Tissue Engineering. In this study, one part of our work aimed at preparing a high strength biohydrogel by using a double-network (DN) hydrogel system, which consisted of two interpenetrating polymer networks composed of γ-glutamic acid, lysine, and alginate, and meanwhile by incorporating bacterial cellulose into the DN structures. The results showed that compression modulus of the resultant hydrogel (0.322 MPa) was comparable with that of natural articular cartilage and swelling degree was greatly depressed by using these strategies. On this basis, a bilayer hydrogel scaffold based on the bionics principle for Osteochondral regeneration was fabricated via chemical and physical cross-linking. Additionally, hydroxyapatite (HA) particles with two different sizes were introduced into the bilayer hydrogels, respectively: micro-HA in the top layer for promoting cartilage matrix deposition and HA nanocrystals in the bottom layer for enhancing compression modulus and osteogenesis. The Osteochondral defect model of rabbits was used to evaluate the repair effect of the scaffolds with the bilayer structure, and the results showed such as-synthesized scaffolds had a good Osteochondral repair effect.
-
a single integrated Osteochondral in situ composite scaffold with a multi layered functional structure
Colloids and Surfaces B: Biointerfaces, 2018Co-Authors: Taijun Chen, Jiafan Bai, Jiajun Tian, Pinhe Huang, Hua Zheng, Jianxin WangAbstract:Abstract This work focuses on the optimization design of a functional biomimetic scaffold for the repair of Osteochondral defects and includes the study of single integrated Osteochondral Tissue Engineering scaffolds with a multi-layered functional structure. Rabbit model experiments were used to evaluate the repair of Osteochondral defects. The results revealed that good integration was achieved both at the interfaces between the scaffold material and the host Tissue and between the newly formed subchondral bone and cartilage. The highest total histological score of 24.2 (based on the modified O’Driscoll scoring system at 12 weeks post-operation) was achieved for Osteochondral repair. The completely repaired cylindrical full-thickness defects for the rabbit animal model reached 5 mm in diameter. The thickness of the regenerated cartilage was almost in line with that of the surrounding normal cartilage, the number and arrangement of cells in the superficial area of cartilage were very close to those of normal hyaline cartilage, and there were clear cartilage lacunas in the regenerated cartilage. The hybrid-use of growth factors and LIPUS stimulation exhibited good potential in enhancing vascularization and the formation of new bone and cartilage, providing better conditions for the overall Osteochondral repair.
-
Biomimetic Bacterial Cellulose-Enhanced Double-Network Hydrogel with Excellent Mechanical Properties Applied for the Osteochondral Defect Repair
2018Co-Authors: Xiangbo Zhu, Taijun Chen, Bo Feng, Jie Weng, Ke Duan, Jianxin WangAbstract:Although hydrogels based on biopolymers show many advantages, their low mechanical properties limit their applications in Osteochondral Tissue Engineering. In this study, one part of our work aimed at preparing a high strength biohydrogel by using a double-network (DN) hydrogel system, which consisted of two interpenetrating polymer networks composed of γ-glutamic acid, lysine, and alginate, and meanwhile by incorporating bacterial cellulose into the DN structures. The results showed that compression modulus of the resultant hydrogel (0.322 MPa) was comparable with that of natural articular cartilage and swelling degree was greatly depressed by using these strategies. On this basis, a bilayer hydrogel scaffold based on the bionics principle for Osteochondral regeneration was fabricated via chemical and physical cross-linking. Additionally, hydroxyapatite (HA) particles with two different sizes were introduced into the bilayer hydrogels, respectively: micro-HA in the top layer for promoting cartilage matrix deposition and HA nanocrystals in the bottom layer for enhancing compression modulus and osteogenesis. The Osteochondral defect model of rabbits was used to evaluate the repair effect of the scaffolds with the bilayer structure, and the results showed such as-synthesized scaffolds had a good Osteochondral repair effect
Anderson Oliveira Lobo - One of the best experts on this subject based on the ideXlab platform.
-
cell viability of porous poly d l lactic acid vertically aligned carbon nanotubes nanohydroxyapatite scaffolds for Osteochondral Tissue Engineering
Materials, 2019Co-Authors: Thiago Domingues Stocco, Eliane Antonioli, Conceicao De Maria Vaz Elias, Bruno V M Rodrigues, Idalia A W B Siqueira, Mario Ferretti, Fernanda Roberta Marciano, Anderson Oliveira LoboAbstract:Treatment of articular cartilage lesions remains an important challenge. Frequently the bone located below the cartilage is also damaged, resulting in defects known as Osteochondral lesions. Tissue Engineering has emerged as a potential approach to treat cartilage and Osteochondral defects. The principal challenge of Osteochondral Tissue Engineering is to create a scaffold with potential to regenerate both cartilage and the subchondral bone involved, considering the intrinsic properties of each Tissue. Recent nanocomposites based on the incorporation of nanoscale fillers into polymer matrix have shown promising results for the treatment of Osteochondral defects. In this present study, it was performed using the recently developed methodologies (electrodeposition and immersion in simulated body fluid) to obtain porous superhydrophilic poly(d,l-lactic acid)/vertically aligned carbon nanotubes/nanohydroxyapatite (PDLLA/VACNT-O:nHAp) nanocomposite scaffolds, to analyze cell behavior and gene expression of chondrocytes, and then assess the applicability of this nanobiomaterial for Osteochondral regenerative medicine. The results demonstrate that PDLLA/VACNT-O:nHAp nanocomposite supports chondrocytes adhesion and decreases type I Collagen mRNA expression. Therefore, these findings suggest the possibility of novel nanobiomaterial as a scaffold for Osteochondral Tissue Engineering applications.
-
Cell Viability of Porous Poly(d,l-lactic acid)/Vertically Aligned Carbon Nanotubes/Nanohydroxyapatite Scaffolds for Osteochondral Tissue Engineering
'MDPI AG', 2019Co-Authors: Thiago Domingues Stocco, Eliane Antonioli, Conceicao De Maria Vaz Elias, Bruno V M Rodrigues, Mario Ferretti, Fernanda Roberta Marciano, Idália Aparecida Waltrick De Brito Siqueira, Anderson Oliveira LoboAbstract:Treatment of articular cartilage lesions remains an important challenge. Frequently the bone located below the cartilage is also damaged, resulting in defects known as Osteochondral lesions. Tissue Engineering has emerged as a potential approach to treat cartilage and Osteochondral defects. The principal challenge of Osteochondral Tissue Engineering is to create a scaffold with potential to regenerate both cartilage and the subchondral bone involved, considering the intrinsic properties of each Tissue. Recent nanocomposites based on the incorporation of nanoscale fillers into polymer matrix have shown promising results for the treatment of Osteochondral defects. In this present study, it was performed using the recently developed methodologies (electrodeposition and immersion in simulated body fluid) to obtain porous superhydrophilic poly(d,l-lactic acid)/vertically aligned carbon nanotubes/nanohydroxyapatite (PDLLA/VACNT-O:nHAp) nanocomposite scaffolds, to analyze cell behavior and gene expression of chondrocytes, and then assess the applicability of this nanobiomaterial for Osteochondral regenerative medicine. The results demonstrate that PDLLA/VACNT-O:nHAp nanocomposite supports chondrocytes adhesion and decreases type I Collagen mRNA expression. Therefore, these findings suggest the possibility of novel nanobiomaterial as a scaffold for Osteochondral Tissue Engineering applications
Jiandong Ding - One of the best experts on this subject based on the ideXlab platform.
-
bilayered plga plga hap composite scaffold for Osteochondral Tissue Engineering and Tissue regeneration
ACS Biomaterials Science & Engineering, 2018Co-Authors: Xiangyu Liang, Pingguo Duan, Jingming Gao, Runsheng Guo, Haoqun Yao, Jiandong DingAbstract:This study is aimed at investigation of the Osteochondral regeneration potential of bilayered PLGA/PLGA-HAp composite scaffolds with one layer made of biodegradable polymer poly(d,l-lactide-co-glycolide) (PLGA) and another layer made of PLGA polymeric matrix coated by bioactive ceramics hydroxyapatite (HAp). The composite scaffolds were fabricated by compression molding/particle leaching and plasma-treated surface deposition. The pore morphology, mechanical properties, and surface deposition of the scaffold were characterized, and the growth of bone marrow derived mesenchymal stem cells or medicinal signaling cells (MSCs) in the scaffold was verified. Thereafter, rabbit models with an artificial Osteochondral defect in joint were randomized into three treatment groups: virgin bilayered scaffold, bilayered scaffold preseeded in vitro with MSCs, and untreated blank control. At 16-week postoperation, both the virgin scaffolds and cell-seeded bilayered scaffolds exhibited Osteochondral repair, as verified by ...
-
Bilayered PLGA/PLGA-HAp Composite Scaffold for Osteochondral Tissue Engineering and Tissue Regeneration
2018Co-Authors: Xiangyu Liang, Pingguo Duan, Jingming Gao, Runsheng Guo, Haoqun Yao, Jiandong DingAbstract:This study is aimed at investigation of the Osteochondral regeneration potential of bilayered PLGA/PLGA-HAp composite scaffolds with one layer made of biodegradable polymer poly(d,l-lactide-co-glycolide) (PLGA) and another layer made of PLGA polymeric matrix coated by bioactive ceramics hydroxyapatite (HAp). The composite scaffolds were fabricated by compression molding/particle leaching and plasma-treated surface deposition. The pore morphology, mechanical properties, and surface deposition of the scaffold were characterized, and the growth of bone marrow derived mesenchymal stem cells or medicinal signaling cells (MSCs) in the scaffold was verified. Thereafter, rabbit models with an artificial Osteochondral defect in joint were randomized into three treatment groups: virgin bilayered scaffold, bilayered scaffold preseeded in vitro with MSCs, and untreated blank control. At 16-week postoperation, both the virgin scaffolds and cell-seeded bilayered scaffolds exhibited Osteochondral repair, as verified by biomechanics analysis, histological evaluations, and Western blot. The results highlighted the potentiality of the bilayered PLGA/PLGA-HAp composite scaffold for Osteochondral Tissue Engineering, and in particular Tissue regeneration or in situ Tissue induction, probably by recruiting the local cells toward chondrogenic and osteogenic differentiation in the porous biomaterials
Taijun Chen - One of the best experts on this subject based on the ideXlab platform.
-
biomimetic bacterial cellulose enhanced double network hydrogel with excellent mechanical properties applied for the Osteochondral defect repair
ACS Biomaterials Science & Engineering, 2018Co-Authors: Xiangbo Zhu, Taijun Chen, Bo Feng, Jie Weng, Ke Duan, Jianxin WangAbstract:Although hydrogels based on biopolymers show many advantages, their low mechanical properties limit their applications in Osteochondral Tissue Engineering. In this study, one part of our work aimed at preparing a high strength biohydrogel by using a double-network (DN) hydrogel system, which consisted of two interpenetrating polymer networks composed of γ-glutamic acid, lysine, and alginate, and meanwhile by incorporating bacterial cellulose into the DN structures. The results showed that compression modulus of the resultant hydrogel (0.322 MPa) was comparable with that of natural articular cartilage and swelling degree was greatly depressed by using these strategies. On this basis, a bilayer hydrogel scaffold based on the bionics principle for Osteochondral regeneration was fabricated via chemical and physical cross-linking. Additionally, hydroxyapatite (HA) particles with two different sizes were introduced into the bilayer hydrogels, respectively: micro-HA in the top layer for promoting cartilage matrix deposition and HA nanocrystals in the bottom layer for enhancing compression modulus and osteogenesis. The Osteochondral defect model of rabbits was used to evaluate the repair effect of the scaffolds with the bilayer structure, and the results showed such as-synthesized scaffolds had a good Osteochondral repair effect.
-
a single integrated Osteochondral in situ composite scaffold with a multi layered functional structure
Colloids and Surfaces B: Biointerfaces, 2018Co-Authors: Taijun Chen, Jiafan Bai, Jiajun Tian, Pinhe Huang, Hua Zheng, Jianxin WangAbstract:Abstract This work focuses on the optimization design of a functional biomimetic scaffold for the repair of Osteochondral defects and includes the study of single integrated Osteochondral Tissue Engineering scaffolds with a multi-layered functional structure. Rabbit model experiments were used to evaluate the repair of Osteochondral defects. The results revealed that good integration was achieved both at the interfaces between the scaffold material and the host Tissue and between the newly formed subchondral bone and cartilage. The highest total histological score of 24.2 (based on the modified O’Driscoll scoring system at 12 weeks post-operation) was achieved for Osteochondral repair. The completely repaired cylindrical full-thickness defects for the rabbit animal model reached 5 mm in diameter. The thickness of the regenerated cartilage was almost in line with that of the surrounding normal cartilage, the number and arrangement of cells in the superficial area of cartilage were very close to those of normal hyaline cartilage, and there were clear cartilage lacunas in the regenerated cartilage. The hybrid-use of growth factors and LIPUS stimulation exhibited good potential in enhancing vascularization and the formation of new bone and cartilage, providing better conditions for the overall Osteochondral repair.
-
Biomimetic Bacterial Cellulose-Enhanced Double-Network Hydrogel with Excellent Mechanical Properties Applied for the Osteochondral Defect Repair
2018Co-Authors: Xiangbo Zhu, Taijun Chen, Bo Feng, Jie Weng, Ke Duan, Jianxin WangAbstract:Although hydrogels based on biopolymers show many advantages, their low mechanical properties limit their applications in Osteochondral Tissue Engineering. In this study, one part of our work aimed at preparing a high strength biohydrogel by using a double-network (DN) hydrogel system, which consisted of two interpenetrating polymer networks composed of γ-glutamic acid, lysine, and alginate, and meanwhile by incorporating bacterial cellulose into the DN structures. The results showed that compression modulus of the resultant hydrogel (0.322 MPa) was comparable with that of natural articular cartilage and swelling degree was greatly depressed by using these strategies. On this basis, a bilayer hydrogel scaffold based on the bionics principle for Osteochondral regeneration was fabricated via chemical and physical cross-linking. Additionally, hydroxyapatite (HA) particles with two different sizes were introduced into the bilayer hydrogels, respectively: micro-HA in the top layer for promoting cartilage matrix deposition and HA nanocrystals in the bottom layer for enhancing compression modulus and osteogenesis. The Osteochondral defect model of rabbits was used to evaluate the repair effect of the scaffolds with the bilayer structure, and the results showed such as-synthesized scaffolds had a good Osteochondral repair effect
