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Marcia Rita Fernandes Machado - One of the best experts on this subject based on the ideXlab platform.
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approach to the humerus and femur of the Paca cuniculus Paca linnaeus 1766
Anatomia Histologia Embryologia, 2021Co-Authors: Leonardo Martins Leal, Marcia Rita Fernandes Machado, Luiz Carlos Vulcano, Tais Harumi De Castro Sasahara, Gabriela Maria Benedetti Vasques, Bruno Watanabe MintoAbstract:The Paca (Cuniculus Paca, Linnaeus 1766) is a rodent species, typical of tropical regions. It is important that in addition to its commercial value as a protein source, it can be used as a scientific scope. The objective of this study was to describe the morphology of the thigh and the arm of the Paca by anatomical and imaging methodology with radiography (X-ray), computed tomography (CAT scan) and magnetic resonance imaging (MRI) to determine the surgical approach of the bones of these regions. It was concluded that the imaging study through X-ray, CAT scan and MRI are reliable methods for anatomical description of wild animals that have little known about their morphology. Finally, similarly to dogs, it was determined that lateral access is the best approach to the femoral diaphysis and the medial access is the best approach to the humerus diaphysis of the Paca.
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morfologia do pâncreas da Paca cuniculus Paca linnaeus 1766
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2019Co-Authors: L C Fiori, Fabrício Singaretti De ,oliveira, Tais Harumi De Castro Sasahara, Leandro Luis Martins, Leonardo Martins Leal, Luciana S Simoes, S Garcia P Filho, Marcia Rita Fernandes MachadoAbstract:RESUMO Este trabalho se baseou na descricao morfologica da Paca (Cuniculus Paca), especie selvagem pertencente a ordem dos roedores, tipica de regioes tropicais, sendo o seu conhecimento anatomico detalhado muito importante para sua conservacao, experimentacao e comercializacao, pois ainda sao escassos na literatura os trabalhos sobre morfologia de especies selvagens. Macroscopicamente, o pâncreas da Paca e formado por lobulos, organizados em ramificacoes difusas, e, microscopicamente, tal orgao possui uma camada de tecido conjuntivo frouxo, a partir da qual partem septos, que dividem a glândula em lobulos, compostos de acinos e ilhotas pancreaticas.
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morfologia e topografia do baco da Paca cuniculus Paca linnaeus 1766
Pesquisa Veterinaria Brasileira, 2017Co-Authors: Isabella F Ribeiro, Fabrício Singaretti De ,oliveira, Marcia Rita Fernandes Machado, Maria Angelica Miglino, Leonardo Martins Leal, Luciana S Simoes, Paola Castro Moraes, Tais Harumi De Castro SasaharaAbstract:RESUMO: A Paca e um grande roedor, presente em parte do territorio brasileiro e na America Latina, cuja importância esta na crescente producao comercial de carne exotica e na pesquisa cientifica como um promissor modelo experimental. Assim, este trabalho objetivou-se descrever a morfologia e a topografia do baco da Paca (Cuniculus Paca). Foram utilizadas cinco Pacas adultas, machos e femeas, fixadas em formaldeido a 10% e armazenados em solucao salina a 30% para sua conservacao. Na Paca, o baco tem sua localizacao relacionada ao estomago, ao figado, ao rim esquerdo, ao pâncreas e ao omento maior. Possui forma irregular, sendo mais longo do que largo, possui coloracao avermelhada-escura e textura lisa. Quanto a descricao histologica, apresenta capsula de tecido conjuntivo denso que emitiu trabeculas que se projetam no parenquima. A capsula e as trabeculas apresentam fibras musculares lisas. O parenquima e composto pela polpa branca e polpa vermelha, esta ultima formada por seios e cordoes esplenicos.
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morfologia do figado da Paca cuniculus Paca linnaeus 1766
Biotemas, 2012Co-Authors: Leandro Luis Martins, Ana Laura Esper Gomes De Figueiredo Carvalho, Andrea Cristina Scarpa Bosso, Marcia Rita Fernandes MachadoAbstract:Morphology of the Paca liver (Cuniculus Paca, Linnaeus 1766). The elements related to the morphology of the liver of Paca (Cuniculus Paca), the second largest rodent of the Brazilian fauna, were observed; this species present zootechnical potential. Eight animals from the animals sector of Faculdade de Ciencias Agrarias e Veterinarias – Campus of Jaboticabal – UNESP, which is duly certified by IBAMA as an experimental breeding institute, were used. Through a dissection procedure, it was found that the liver of the Paca is located in the cranial portion of the abdomen, immediately after the diaphragm, to which it is connected by the triangular,
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Morphology of the Paca liver (Cuniculus Paca, Linnaeus 1766)
Universidade Federal de Santa Catarina, 2012Co-Authors: Ana Laura Esper Gomes De Figueiredo Carvalho, Leandro Luis Martins, Andrea Cristina Scarpa Bosso, Marcia Rita Fernandes MachadoAbstract:The elements related to the morphology of the liver of Paca (Cuniculus Paca), the second largest rodent of the Brazilian fauna, were observed; this species present zootechnical potential. Eight animals from the animals sector of Faculdade de Ciencias Agrarias e Veterinarias – Campus of Jaboticabal – UNESP, which is duly certified by IBAMA as an experimental breeding institute, were used. Through a dissection procedure, it was found that the liver of the Paca is located in the cranial portion of the abdomen, immediately after the diaphragm, to which it is connected by the triangular, coronary, and falciform ligaments, having its bigger part located right to the medium plan. The liver of this rodent presents the following lobation: right lateral lobe, right medial lobe, quadrate lobe, left medial lobe, and left lateral lobe, besides the caudate lobe formed by the papillary process of caudate lobe and the caudate process of caudate lobe. Gallbladder is located between the quadrate and right medial lobes. Fragments of this organ were collected, fixed, and histologically prepared, being the samples analyzed through light microscopy. It was microscopically observed that intralobular connective tissue is scarce, basically it consists polyhedral hepatocytes organized into cords interposed between sinusoids and the portal triads are found in the lobe, consisting of the portal vein, hepatic artery, and biliary duct
Tomiyasu Murata - One of the best experts on this subject based on the ideXlab platform.
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the flavonoid p hydroxycinnamic acid exhibits anticancer effects in human pancreatic cancer mia Paca 2 cells in vitro comparison with gemcitabine
Oncology Reports, 2015Co-Authors: Masayoshi Yamaguchi, Tomiyasu Murata, Bassel F Elrayes, Mamoru ShojiAbstract:Pancreatic cancer is a highly aggressive malignancy with a notoriously dismal prognosis. A major contributor to this poor clinical outcome is pancreatic cancer's prominent chemoresistance. The present study was undertaken to determine whether the flavonoid p‑hydroxycinnamic acid (HCA), which is a botanical factor, possesses anticancer effects on cloned human pancreatic cancer MIA Paca‑2 cells that possess resistance to radiation therapy in vitro. Proliferation of MIA Paca‑2 cells was suppressed after culture with HCA (10‑1,000 nM). Such an effect was also noted in human pancreatic cancer Pt45P1 cells. In the MIA Paca‑2 cells, HCA induced G1 and G2/M phase cell cycle arrest in the cells. The suppressive effects of HCA on proliferation were suggested to be mediated through the inhibition of various signaling pathways related to nuclear factor‑κB (NF‑κB), extracellular signal‑regulated kinase (ERK), protein kinase C, phosphatidylinositol 3‑kinase (PI3K) or nuclear transcription activity. Moreover, HCA was found to stimulate cell death in the MIA Paca‑2 and Pt45P1 cells in vitro. The anticancer effects of HCA on MIA Paca‑2 cells were exhibited at a lower concentration than gemcitabine, a potent cancer drug. The flavonoid HCA may be a useful tool in the therapy of human pancreatic cancer in vivo.
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suppressive effects of exogenous regucalcin on the proliferation of human pancreatic cancer mia Paca 2 cells in vitro
International Journal of Molecular Medicine, 2015Co-Authors: Masayoshi Yamaguchi, Tomiyasu MurataAbstract:Regucalcin plays a pivotal role as a suppressor protein in signal transduction in various types of cells and tissues. The regucalcin gene is localized on the X chromosome. and its expression has been shown to be suppressed in various types of tumor tissue in animal and human subjects, suggesting a potential role of regucalcin in carcinogenesis. This study was undertaken to determine the effects of exogenous regucalcin on the proliferation of cloned human pancreatic cancer MIA Paca-2 cells in vitro. The proliferation of the MIA Paca-2 cells was suppressed following culture with regucalcin (0.01-10 nM). Such an effect was also observed in pancreatic cancer Pt45P1 cells, that highly expressed tissue factor (high TF), or Pt45P1 cells, that highly expressed alternativly spliced variants of tissue factor (asTF). In the MIA Paca-2 cells, the suppressive effects of regucalcin on cell proliferation were not enhanced either in the presence of tumor necrosis factor-α (TNF-α), or in the presence of Bay K 8644, PD98059, staurosporine, wortmannin or 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). However, this was not the case for gemcitabine, which was shown to suppress cell proliferation. Exogenous regucalcin did not cause apoptotic cell death in the MIA Paca-2 cells in vitro. These findings demonstrate that exogenous regucalcin exerts suppressive effects on the proliferation of human pancreatic MIA Paca-2 cells and that these effects are mediated through the inhibition of various signaling pathways related to nuclear factor-κB (NF-κB), extracellular signal-regulated kinase (ERK), protein kinase C, calcium signaling, phosphatidylinositol 3-kinase (PI3K) or nuclear transcription activity in vitro. Our data suggest that exogenous regucalcin exerts suppressive effects on the proliferation of human pancreatic cancer cells.
Masayoshi Yamaguchi - One of the best experts on this subject based on the ideXlab platform.
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the flavonoid p hydroxycinnamic acid exhibits anticancer effects in human pancreatic cancer mia Paca 2 cells in vitro comparison with gemcitabine
Oncology Reports, 2015Co-Authors: Masayoshi Yamaguchi, Tomiyasu Murata, Bassel F Elrayes, Mamoru ShojiAbstract:Pancreatic cancer is a highly aggressive malignancy with a notoriously dismal prognosis. A major contributor to this poor clinical outcome is pancreatic cancer's prominent chemoresistance. The present study was undertaken to determine whether the flavonoid p‑hydroxycinnamic acid (HCA), which is a botanical factor, possesses anticancer effects on cloned human pancreatic cancer MIA Paca‑2 cells that possess resistance to radiation therapy in vitro. Proliferation of MIA Paca‑2 cells was suppressed after culture with HCA (10‑1,000 nM). Such an effect was also noted in human pancreatic cancer Pt45P1 cells. In the MIA Paca‑2 cells, HCA induced G1 and G2/M phase cell cycle arrest in the cells. The suppressive effects of HCA on proliferation were suggested to be mediated through the inhibition of various signaling pathways related to nuclear factor‑κB (NF‑κB), extracellular signal‑regulated kinase (ERK), protein kinase C, phosphatidylinositol 3‑kinase (PI3K) or nuclear transcription activity. Moreover, HCA was found to stimulate cell death in the MIA Paca‑2 and Pt45P1 cells in vitro. The anticancer effects of HCA on MIA Paca‑2 cells were exhibited at a lower concentration than gemcitabine, a potent cancer drug. The flavonoid HCA may be a useful tool in the therapy of human pancreatic cancer in vivo.
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suppressive effects of exogenous regucalcin on the proliferation of human pancreatic cancer mia Paca 2 cells in vitro
International Journal of Molecular Medicine, 2015Co-Authors: Masayoshi Yamaguchi, Tomiyasu MurataAbstract:Regucalcin plays a pivotal role as a suppressor protein in signal transduction in various types of cells and tissues. The regucalcin gene is localized on the X chromosome. and its expression has been shown to be suppressed in various types of tumor tissue in animal and human subjects, suggesting a potential role of regucalcin in carcinogenesis. This study was undertaken to determine the effects of exogenous regucalcin on the proliferation of cloned human pancreatic cancer MIA Paca-2 cells in vitro. The proliferation of the MIA Paca-2 cells was suppressed following culture with regucalcin (0.01-10 nM). Such an effect was also observed in pancreatic cancer Pt45P1 cells, that highly expressed tissue factor (high TF), or Pt45P1 cells, that highly expressed alternativly spliced variants of tissue factor (asTF). In the MIA Paca-2 cells, the suppressive effects of regucalcin on cell proliferation were not enhanced either in the presence of tumor necrosis factor-α (TNF-α), or in the presence of Bay K 8644, PD98059, staurosporine, wortmannin or 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). However, this was not the case for gemcitabine, which was shown to suppress cell proliferation. Exogenous regucalcin did not cause apoptotic cell death in the MIA Paca-2 cells in vitro. These findings demonstrate that exogenous regucalcin exerts suppressive effects on the proliferation of human pancreatic MIA Paca-2 cells and that these effects are mediated through the inhibition of various signaling pathways related to nuclear factor-κB (NF-κB), extracellular signal-regulated kinase (ERK), protein kinase C, calcium signaling, phosphatidylinositol 3-kinase (PI3K) or nuclear transcription activity in vitro. Our data suggest that exogenous regucalcin exerts suppressive effects on the proliferation of human pancreatic cancer cells.
Bharat B. Aggarwal - One of the best experts on this subject based on the ideXlab platform.
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Boswellic Acid Suppresses Growth and Metastasis of Human Pancreatic Tumors in an Orthotopic Nude Mouse Model through Modulation of Multiple Targets
2013Co-Authors: Byoungduck Park, Bokyung Sung, Sahdeo Prasad, Vivek Yadav, Bharat B. AggarwalAbstract:Pancreatic cancer (Paca) is one of the most lethal cancers, with an estimated 5-year survival of,5 % even when patients are given the best treatment available. In addition, these treatments are often toxic and expensive, thus new agents which are safe, affordable and effective are urgently needed. We describe here the results of our study with acetyl-11-keto-b-boswellic acid (AKBA), an agent obtained from an Ayurvedic medicine, gum resin of Boswellia serrata. Whether AKBA has an activity against human Paca, was examined in in vitro models and in an orthotopic nude mouse model of Paca. We found that AKBA inhibited the proliferation of four different Paca cell lines (AsPC-1, PANC-28, and MIA Paca-2 with K-Ras and p53 mutations, and BxPC-3 with wild-type K-Ras and p53 mutation). These effects correlated with an inhibition of constitutively active NF-kB and suppression of NF-kB regulating gene expression. AKBA also induced apoptosis, and sensitized the cells to apoptotic effects of gemcitabine. In the orthotopic nude mouse model of Paca, p.o. administration of AKBA alone (100 mg/ kg) significantly inhibited the tumor growth; this activity was enhanced by gemcitabine. In addition, AKBA inhibited the metastasis of the Paca to spleen, liver, and lungs. This correlated with decreases in Ki-67, a biomarker of proliferation, and CD31, a biomarker of microvessel density, in the tumor tissue. AKBA produced significant decreases in the expression of NFkB regulating genes in the tissues. Immunohistochemical analysis also showed AKBA downregulated the expression of COX-2, MMP-9, CXCR4, and VEGF in the tissues. Overall these results demonstrate that AKBA can suppress the growth an
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boswellic acid suppresses growth and metastasis of human pancreatic tumors in an orthotopic nude mouse model through modulation of multiple targets
PLOS ONE, 2011Co-Authors: Byoungduck Park, Bokyung Sung, Sahdeo Prasad, Vivek R Yadav, Bharat B. AggarwalAbstract:Pancreatic cancer (Paca) is one of the most lethal cancers, with an estimated 5-year survival of <5% even when patients are given the best treatment available. In addition, these treatments are often toxic and expensive, thus new agents which are safe, affordable and effective are urgently needed. We describe here the results of our study with acetyl-11-keto-β-boswellic acid (AKBA), an agent obtained from an Ayurvedic medicine, gum resin of Boswellia serrata. Whether AKBA has an activity against human Paca, was examined in in vitro models and in an orthotopic nude mouse model of Paca. We found that AKBA inhibited the proliferation of four different Paca cell lines (AsPC-1, PANC-28, and MIA Paca-2 with K-Ras and p53 mutations, and BxPC-3 with wild-type K-Ras and p53 mutation). These effects correlated with an inhibition of constitutively active NF-κB and suppression of NF-κB regulating gene expression. AKBA also induced apoptosis, and sensitized the cells to apoptotic effects of gemcitabine. In the orthotopic nude mouse model of Paca, p.o. administration of AKBA alone (100 mg/kg) significantly inhibited the tumor growth; this activity was enhanced by gemcitabine. In addition, AKBA inhibited the metastasis of the Paca to spleen, liver, and lungs. This correlated with decreases in Ki-67, a biomarker of proliferation, and CD31, a biomarker of microvessel density, in the tumor tissue. AKBA produced significant decreases in the expression of NF-κB regulating genes in the tissues. Immunohistochemical analysis also showed AKBA downregulated the expression of COX-2, MMP-9, CXCR4, and VEGF in the tissues. Overall these results demonstrate that AKBA can suppress the growth and metastasis of human pancreatic tumors in an orthotopic nude mouse model that correlates with modulation of multiple targets.
Jianhui Rong - One of the best experts on this subject based on the ideXlab platform.
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n propargyl caffeate amide Paca prevents cardiac fibrosis in experimental myocardial infarction by promoting pro resolving macrophage polarization
Aging, 2020Co-Authors: Yuanyuan Cheng, Jianhui Rong, Dan Luo, Yingke ZhaoAbstract:Macrophages control the initiation and resolution of cardiac fibrosis in post-infarction cardiac remodeling. The aim of the present study was to investigate whether N-propargyl caffeate amide (Paca) could suppress myocardial fibrosis via regulating macrophage polarization. By using rat model of isoproterenol-induced myocardial fibrosis, we discovered that Paca could reduce cardiac fibrosis in a dose-dependent manner. To elucidate the anti-fibrotic mechanisms, we examined whether Paca affected pro-inflammatory M1 and pro-resolving macrophage biomarkers in macrophage polarization. As result, Paca reduced the expression of pro-inflammatory M1 biomarkers (e.g., iNOS, TNF-α, CXCL10, IL-6, CCL2 and CD80) while increased the expression of pro-resolving M2a biomarkers (e.g., IL-10, arginase-1, FZZ1, YM-1 and CD163) in LPS-stimulated RAW264.7 macrophages. Paca also suppressed the elevation of M1 biomarker ED1 in the early phase but up-regulated the expression of pro-resolving biomarker ED2 in the later phase. Moreover, Paca reduced the expression of pro-fibrotic TGF-β1 and PDGF-α while maintained or even increased the production of pro-apoptotic MMP-13, MMP-9 and TRAIL. Importantly, mechanistic studies revealed that Paca might promote the switch of macrophage polarization towards a pro-resolving macrophage phenotype via activating PPAR-γ pathway. Taken together, this study suggested that Paca might be a drug candidate for preventing cardiac fibrosis in myocardial infarction.
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n propargyl caffeamide Paca ameliorates dopaminergic neuronal loss and motor dysfunctions in mptp mouse model of parkinson s disease and in mpp induced neurons via promoting the conversion of prongf to ngf
Molecular Neurobiology, 2018Co-Authors: Dan Luo, Jia Zhao, Yuanyuan Cheng, Simon Mingyuen Lee, Jianhui RongAbstract:Insufficient production of nerve growth factor (NGF) is implicated in Parkinson’s disease (PD). We recently discovered that caffeic acid derivative N-propargyl caffeamide (Paca) not only potentiated NGF-induced neurite outgrowth but also attenuated 6-hydroxydopamine neurotoxicity in neuronal culture. The aim of the present study was to investigate whether Paca could increase NGF levels against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) neurotoxicity in a mouse PD model. We induced parkinsonism in mice by intraperitoneal injection of MPTP for seven consecutive days. Animal motor functions were assessed by rotarod test and pole test. Our results showed that Paca ameliorated motor impairments in MPTP-challenged mice. Based on Western blot analysis and/or immunofluorescence staining of NGF and tyrosine hydroxylase (TH), Paca preserved TH levels in the midbrain substantia nigra pars compacta. Paca also increased NGF expression while it decreased proNGF accumulation. Interestingly, NGF was widely induced in the midbrains including astrocytes. To elucidate the mechanisms by which Paca induces NGF, we focused on the effects of Paca on two neurotrophic signaling pathways, the PI3K and MEK pathways. We found that Paca induced the phosphorylation of Akt, ERK, and CREB against MPTP-mediated alterations. Importantly, Paca increased NGF levels and subsequently induced TrkA activation in MPTP-treated mice. Consistently, Paca also increased NGF levels in dopaminergic PC12 cells and primary rat midbrain neurons against N-methyl-4-phenylpyridinium iodide (MPP+) toxicity. ERK and PI3K inhibitors attenuated the effects of Paca on NGF levels. Collectively, our results suggest that Paca may rescue NGF insufficiency via sequential activation of PI3K/Akt, ERK1/2, and CREB signaling pathways.
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n propargyl caffeate amide Paca potentiates nerve growth factor ngf induced neurite outgrowth and attenuates 6 hydroxydopamine 6 ohda induced toxicity by activating the nrf2 ho 1 pathway
ACS Chemical Neuroscience, 2015Co-Authors: Chuanbin Yang, Jia Zhao, Yuanyuan Cheng, Jianhui RongAbstract:Insufficient production of neurotrophic factors is implicated in the pathogenesis of various neurodegenerative disorders. The aim of the present study was to evaluate the potential of N-propargyl caffeate amide (Paca) to enhance nerve growth factor (NGF)-induced neurite outgrowth and the underlying mechanisms. We discovered that Paca not only potentiated NGF-induced neurite outgrowth but also attenuated 6-hydroxydopamine (6-OHDA) neurotoxicity in dopaminergic PC12 cells and primary rat midbrain neurons. To identify the Paca-binding proteins, we introduced a biotin tag to the covalent Paca-protein adducts via "click chemistry" alkyne-azido cycloaddition. As a result, kelch-like ECH-associated protein 1 (Keap1) was isolated as the predominant protein from Paca treated PC12 cells. We demonstrated that the formation of Paca-Keap1 conjugates induced the nuclear translocation of transcription factor Nrf2 and the expression of antioxidant heme oxygenase-1 (HO-1). Importantly, specific HO-1 inhibitor SnPP diminished the neuroprotective and neuritogenic activities of Paca. Moreover, Paca attenuated 6-OHDA-induced production of neurotoxic reactive oxygen species and reactive nitrogen species. Paca also preserved mitochondrial membrane integrity and enhanced the cellular resistance against 6-OHDA neurotoxicity. These results suggest that Paca may exhibit neuroprotective and neuritogenic activities via activating the Nrf2/HO-1 antioxidant pathway.
