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José Floriano Barêa Pastore - One of the best experts on this subject based on the ideXlab platform.
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two new endangered species of Polygala series trichospermae Polygalaceae endemic to the cerrado domain tocantins state brazil
European journal of taxonomy, 2021Co-Authors: José Floriano Barêa Pastore, Guilherme Medeiros AntarAbstract:The Cerrado domain is the most diverse savannah biome in the world and a biodiversity hotspot for conservation. Only a few areas of the domain remain well preserved, among them the Jalapao region in Tocantins State, currently is considered the best preserved area of the Brazilian Cerrado. Listed in a recent Angiosperm checklist for the region, there were two species of Polygala series Trichospermae, which were recognized as new by the first author. Polygala bringelii sp. nov., endemic to the Jalapao region, is diagnosed by its obclavate seeds without a ring of trichomes at their base, keel with the crest shorter than the cuculus, and the internal sepals (wings) longer than the capsule. Polygala tocantinensis sp. nov., endemic to Tocantins state, is distinguished by its internal sepals (wings) with two glands near to the rounded apex, and a ring of convergent trichomes at the base of the seeds. The two species are morphologically closest with Polygala trichosperma of sect. Timutua, series Trichospermae, but differs in its corolla and seeds shape and pubescence. An identification key, photographic plates, photos of floral parts under magnification, preliminary conservation assessments, and ecological comments of both species are also provided.
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one for all molecular study of Polygala major complex Polygalaceae in southwest asia
Plant Systematics and Evolution, 2019Co-Authors: Dmitry Lyskov, José Floriano Barêa Pastore, Tahir H SamigullinAbstract:Ten species of Polygala closely related to P. major are recorded in Southwest Asia. Most of them were described as local endemics of the Transcaucasia. However, species of the P. major complex are often difficult to identify, having rather subtle and changeable morphological features that could not be treated as essential. The phylogenetic analysis of sequences of the nrITS/ETS and chloroplast trnL–trnF regions (intron and spacer) showed their extreme similarity. Based on the molecular evidence and main diagnostic morphological features, the ten species of the P. major complex are synonymized under P. major. Typifications of eight combinations in Polygala from Western Asia and Europe are also clarified.
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typification of names and nomenclatural notes on the south andean and patagonian species of Polygala linnaeus Polygalaceae
Phytotaxa, 2018Co-Authors: Agustina Martinez, Fernando O Zuloaga, José Floriano Barêa PastoreAbstract:As part of an ongoing revision of the genus Polygala , several names of the South Andean and Patagonian species in need of nomenclatural and taxonomic study were identified. 13 lectotypes have been designated for accepted names, synonyms and a dubious taxon.
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Polygala pubescens muhl Polygalaceae as a replacement name for p senega l var rosea michx
Phytotaxa, 2018Co-Authors: José Floriano Barêa Pastore, Michelle MotaAbstract:Polygala pubescens Muhl., often treated as an invalid name (as nomen nudum ), is here considered validly published and the legitimate name for Polygala senega L. var. rosea Michx. Establishment of the validity of P. pubescens Muhl. (1813), a taxonomic synonym of Asemeia grandiflora (Walter) Small, makes P. pubescens Mart. (1815) a taxonomic synonym of P. erioptera DC. (1824), a later homonym. Muhlenberg’s names published in two editions of his Catalogus Plantarum Americae Septentrionalis (1813, 1818) are analyzed and discussed. A lectotype for Polygala pubescens Rottler ex Mart. is here designated.
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Polygala veadeiroensis Polygalaceae a new species of Polygala endemic to chapada dos veadeiros goias brazil
Kew Bulletin, 2018Co-Authors: José Floriano Barêa PastoreAbstract:Summary. A new species, Polygala veadeiroensis (Polygalaceae) subgenus Polygala section Timutua DC. series Timoutoideae, is described from Chapada dos Veadeiros, Goias State, Brazil. The infrageneric placement and its conservation status are assessed and its habitat and phenology are included. The new species is compared with its morphologically allied species, P. carphoides and P. cuspidata. Illustrations, photos of floral parts under magnification, and a distribution map are also provided.
Moacir Geraldo Pizzolatti - One of the best experts on this subject based on the ideXlab platform.
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dihydrostyryl 2 pyrone as a chemical marker of three non xanthone producing Polygala species Polygalaceae
Biochemical Systematics and Ecology, 2020Co-Authors: Tiago Tizziani, Dalila Venzke, Ana Paula Ruani, Gustavo Amadeu Micke, Moacir Geraldo Pizzolatti, Ines Maria Costa BrighenteAbstract:Abstract The phytochemical investigation of the ethyl acetate fraction of Polygala longicaulis Kunth resulted in the isolation and identification of two dihydrostyryl-2-pyrones (1–2). This is the third species of the Polygala L. genus, along with Polygala altomontana Ludtke, Boldrini & Miotto and Polygala sabulosa A.W. Benn., to present the accumulation of dihydrostyryl-2-pyrones. The data reported herein make an important contribution to the chemotaxonomy of this genus. This is the first report of dihydrostyryl-2-pyrone (1) and dihydrometisticin (2) in P. longicaulis and the first report of dihydrometisticin in the genus Polygala. Furthermore, this is the first grouping of non-xanthone-producing Polygala species.
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a new xanthone as a chemical marker of four Polygala species Polygalaceae
Biochemical Systematics and Ecology, 2018Co-Authors: Tiago Tizziani, Dalila Venzke, Ana Paula Ruani, Marcos Pereira, Gustavo Amadeu Micke, Moacir Geraldo Pizzolatti, Fabiana Cristina Missau, Daiane Fiuza Montagner, Ines Maria Costa BrighenteAbstract:Abstract A new xanthone, 1,5-dihydroxy-2,3-methylenedioxyxanthone (1), was isolated from four species of Polygala (Polygala campestris, Polygala densiracemosa, Polygala linoides and Polygala pulchella). In addition, three other xanthones and one sterol were isolated from extracts of these species: 1,5-dihydroxy-2,3-dimethoxyxanthone (2), 1,5-dihydroxy-3-methoxyxanthone (3), 1,5-dihydroxy-6′,6′dimethylpyrano[2′,3':3,2]xanthone (4) and α-spinasterol (5). The xanthone 1 is new in the literature and although the others are known compounds this is the first time they have been isolated from these species. The chemotaxonomic significance of these data for the Polygala genus is reported.
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phytochemical and chemotaxonomic study of Polygala altomontana Polygalaceae
Biochemical Systematics and Ecology, 2018Co-Authors: Tiago Tizziani, Dalila Venzke, Ana Paula Ruani, Marcos Pereira, Gustavo Amadeu Micke, Moacir Geraldo Pizzolatti, Ines Maria Costa BrighenteAbstract:Abstract In this study, 7 compounds were isolated from a hydroalcoholic extract of Polygala altomontana: aurapten (1), α-spinasterol (2) dihydrostyryl-2-pyrone (3) and styryl-2-pyrones (4–7). The styryl-2-pyrone (5) is new in the literature and although the others are known compounds this is the first time they have been isolated from the species P. altomontana. This is the second species of the genus Polygala to present the accumulation of dihydrostyryl-2-pyrones and styryl-2-pyrones. The chemotaxonomic significance of this data for the Polygala genus was summarized.
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protective effects of Polygala paniculata extract against methylmercury induced neurotoxicity in mice
Journal of Pharmacy and Pharmacology, 2010Co-Authors: Marcelo Farina, Fabiana Cristina Missau, Jeferson Luis Franco, Camila M Ribas, Flavia Carla Meotti, Alcir Luiz Dafre, Adair R S Santos, Moacir Geraldo PizzolattiAbstract:We have examined the possible protective effects of Polygala paniculata extract against methylmercury (MeHg)-induced neurotoxicity in adult mice. MeHg was diluted in drinking water (40 mg L - 1 , freely available) and thehydroalcoholic Polygala extract was diluted in a 150 mM NaCI solution and administered by gavage (100 mg kg - 1 b.w., twice a day). After a two-week treatment, MeHg exposure significantly inhibited glutathione peroxidase and increased glutathione reductase activity, while the levels of thiobarbituric acid reactive substances were increased in the cerebral cortex and cerebellum. These alterations were prevented by administration of Polygala extract, except for glutathione reductase activity, which remained elevated in the cerebral cortex. Behavioural interference in the MeHg-exposed animals was evident through a marked deficit in the motor performance in the rotarod task, which was completely recovered to control levels by Polygala extract co-administration. This study has shown, for the first time, the in-vivo protective effects of Polygala extract against MeHg-induced neurotoxicity. In addition, our findings encourage studies concerning the beneficial effects of P. paniculata on neurological conditions related to excitotoxicity and oxidative stress.
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anticonvulsant and anxiolytic like effects of compounds isolated from Polygala sabulosa Polygalaceae in rodents in vitro and in vivo interactions with benzodiazepine binding sites
Psychopharmacology, 2008Co-Authors: Filipe S Duarte, Moacir Geraldo Pizzolatti, Mariel Marder, Alexandre Ademar Hoeller, Marcelo Duzzioni, Beatriz G Mendes, Thereza C M De LimaAbstract:Rationale Polygala sabulosa, a folk medicine, presents dihydrostyryl-2-pyrones (DST) and styryl-2-pyrones (STY), compounds structurally similar to kavalactones. Our previous study showed that the ethyl acetate fraction (EA) and these constituents present anxiolytic-like, hypno-sedative, and anticonvulsant effects in mice.
Xuemei Qin - One of the best experts on this subject based on the ideXlab platform.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid
2016Co-Authors: Saponin Biosynthesis Of Polygala Tenuifolia, Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background: The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear. Methodology/Findings: In this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP.1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (b-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis. Conclusions/Significance: This finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gen
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uplc q tof ms based metabolomics and qrt pcr in enzyme gene screening with key role in triterpenoid saponin biosynthesis of Polygala tenuifolia
PLOS ONE, 2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid Saponin Biosynthesis of Polygala tenuifolia
2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:BackgroundThe dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.Methodology/FindingsIn this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP>1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (β-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis.Conclusions/SignificanceThis finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gene expression analysis can effectively elucidate the mechanism of triterpenoid saponin biosynthesis and can provide useful information on gene discovery. These findings can serve as a reference for using the overexpression of genes encoding for SQS, SQE, and/or β-AS to increase the triterpenoid saponin production of P. tenuifolia.
Fusheng Zhang - One of the best experts on this subject based on the ideXlab platform.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid
2016Co-Authors: Saponin Biosynthesis Of Polygala Tenuifolia, Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background: The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear. Methodology/Findings: In this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP.1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (b-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis. Conclusions/Significance: This finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gen
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uplc q tof ms based metabolomics and qrt pcr in enzyme gene screening with key role in triterpenoid saponin biosynthesis of Polygala tenuifolia
PLOS ONE, 2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid Saponin Biosynthesis of Polygala tenuifolia
2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:BackgroundThe dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.Methodology/FindingsIn this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP>1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (β-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis.Conclusions/SignificanceThis finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gene expression analysis can effectively elucidate the mechanism of triterpenoid saponin biosynthesis and can provide useful information on gene discovery. These findings can serve as a reference for using the overexpression of genes encoding for SQS, SQE, and/or β-AS to increase the triterpenoid saponin production of P. tenuifolia.
Bing Peng - One of the best experts on this subject based on the ideXlab platform.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid
2016Co-Authors: Saponin Biosynthesis Of Polygala Tenuifolia, Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background: The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear. Methodology/Findings: In this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP.1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (b-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis. Conclusions/Significance: This finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gen
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uplc q tof ms based metabolomics and qrt pcr in enzyme gene screening with key role in triterpenoid saponin biosynthesis of Polygala tenuifolia
PLOS ONE, 2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:Background The dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.
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UPLC/Q-TOF MS-Based Metabolomics and qRT-PCR in Enzyme Gene Screening with Key Role in Triterpenoid Saponin Biosynthesis of Polygala tenuifolia
2014Co-Authors: Fusheng Zhang, Bing Peng, Xuemei QinAbstract:BackgroundThe dried root of Polygala tenuifolia, named Radix Polygalae, is a well-known traditional Chinese medicine. Triterpenoid saponins are some of the most important components of Radix Polygalae extracts and are widely studied because of their valuable pharmacological properties. However, the relationship between gene expression and triterpenoid saponin biosynthesis in P. tenuifolia is unclear.Methodology/FindingsIn this study, ultra-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF MS)-based metabolomic analysis was performed to identify and quantify the different chemical constituents of the roots, stems, leaves, and seeds of P. tenuifolia. A total of 22 marker compounds (VIP>1) were explored, and significant differences in all 7 triterpenoid saponins among the different tissues were found. We also observed an efficient reference gene GAPDH for different tissues in this plant and determined the expression level of some genes in the triterpenoid saponin biosynthetic pathway. Results showed that MVA pathway has more important functions in the triterpenoid saponin biosynthesis of P. tenuifolia. The expression levels of squalene synthase (SQS), squalene monooxygenase (SQE), and beta-amyrin synthase (β-AS) were highly correlated with the peak area intensity of triterpenoid saponins compared with data from UPLC/Q-TOF MS-based metabolomic analysis.Conclusions/SignificanceThis finding suggested that a combination of UPLC/Q-TOF MS-based metabolomics and gene expression analysis can effectively elucidate the mechanism of triterpenoid saponin biosynthesis and can provide useful information on gene discovery. These findings can serve as a reference for using the overexpression of genes encoding for SQS, SQE, and/or β-AS to increase the triterpenoid saponin production of P. tenuifolia.
