The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Yumei Zhang - One of the best experts on this subject based on the ideXlab platform.
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vegf flk1 mechanism is involved in Roxarsone promotion of rat endothelial cell growth and b16f10 xenograft tumor angiogenesis
Scientific Reports, 2019Co-Authors: Shihao Chen, Qianhan Wei, Zeting Zhao, Xin Chen, Hengmi Cui, Yumei ZhangAbstract:The potential angiogenic effect of Roxarsone, a feed additive widely used to promote animal growth worldwide, was demonstrated recently. We explored the mechanism of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in Roxarsone promotion of rat vascular endothelial cells (ECs) and B16F10 mouse xenografts. ECs were treated with 0.1–50 μM Roxarsone or with Roxarsone plus 10 ng/mL VEGF, VEGFR1 (Flt1), or VEGFR2 (Flk1) antibodies for 12–48 h to examine their role in cell growth promotion. Small interfering RNA (siRNA) targeting Vegf, Flt1, and Flk1 were transfected in the ECs, and we measured the expression level, cell proliferation, migration, and tube formation ability. The siRNA targeting Vegf or Flk1 were injected intratumorally in the B16F10 xenografts of mice that received 25 mg/kg Roxarsone orally. Cell viability and VEGF expression following Roxarsone treatment were significantly higher than that of the control (P < 0.05), peaking following treatment with 1.0 μM Roxarsone. Compared to Roxarsone alone, the VEGF antibody decreased cell promotion by Roxarsone (P < 0.05), and the Flk1 antibody greatly reduced cell viability compared to the Flt1 antibody (P < 0.01). Roxarsone and Flk1 antibody co-treatment increased supernatant VEGF significantly, while cellular VEGF was obviously decreased (P < 0.01), whereas there was no significant difference following Flt1 antibody blockade. The siRNA against Vegf or Flk1 significantly attenuated the Roxarsone promotion effects on EC proliferation, migration, and tube-like formation (P < 0.01), whereas the siRNA against Flt1 effected no obvious differences. Furthermore, the RNA interference significantly weakened the Roxarsone-induced increase in xenograft weight and volume, and VEGF and Flk1 expression. Roxarsone promotion of rat EC growth, migration, and tube-like formation in vitro and of B16F10 mouse xenograft model tumor growth and angiogenesis involves a VEGF/Flk1 mechanism.
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VEGF/Flk1 Mechanism is Involved in Roxarsone Promotion of Rat Endothelial Cell Growth and B16F10 Xenograft Tumor Angiogenesis
Scientific reports, 2019Co-Authors: Shihao Chen, Qianhan Wei, Zeting Zhao, Xin Chen, Hengmi Cui, Yumei ZhangAbstract:The potential angiogenic effect of Roxarsone, a feed additive widely used to promote animal growth worldwide, was demonstrated recently. We explored the mechanism of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in Roxarsone promotion of rat vascular endothelial cells (ECs) and B16F10 mouse xenografts. ECs were treated with 0.1–50 μM Roxarsone or with Roxarsone plus 10 ng/mL VEGF, VEGFR1 (Flt1), or VEGFR2 (Flk1) antibodies for 12–48 h to examine their role in cell growth promotion. Small interfering RNA (siRNA) targeting Vegf, Flt1, and Flk1 were transfected in the ECs, and we measured the expression level, cell proliferation, migration, and tube formation ability. The siRNA targeting Vegf or Flk1 were injected intratumorally in the B16F10 xenografts of mice that received 25 mg/kg Roxarsone orally. Cell viability and VEGF expression following Roxarsone treatment were significantly higher than that of the control (P
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tumor promoting and pro angiogenic effects of Roxarsone via vegfr2 plcγ pkc signaling
Chemico-Biological Interactions, 2018Co-Authors: Yanhua Pang, Yujing Wang, Kai Wang, Zhongri Chenlin, Weiwei Lei, Yumei ZhangAbstract:Abstract Roxarsone is an organoarsenic feed additive used in livestock and poultry production that is released into the environment, where it poses a risk to human health. It is known to have a tumor-promoting effect that is brought about by pro-angiogenic factors such as vascular endothelial growth factor (VEGF) and it receptors (VEGFR). However, little information is available about the other signaling molecules that could be involved. This study aims to investigate the role of PLCγ/PKC signaling in Roxarsone-induced angiogenesis in a mouse B16-F10 melanoma xenograft model and rat vascular endothelial cells (ECs). Results showed treatment with 5 mg/kg and 25 mg/kg Roxarsone resulted in an obvious increase in the weight and volume of B16-F10 xenografts and PLCγ/PKC phosphorylation in a dose-dependent manner in C57BL/6 mice. SU5416, a VEGFR2 inhibitor, significantly attenuated the tumor growth induced by Roxarsone. Further, 1.0 μmol/L Roxarsone treatment in rat ECs was observed to significantly increase the optical density rate in the MTT assay, the number of BrdU-positive cells in the proliferation assay, the migration distance in the scratch test, and the number of meshes formed in the tube formation assay. In addition, treatment with 1.0 μmol/L Roxarsone was associated with significantly higher phosphorylation of PLCγ/PKC than the control treatment. U73122, a PLCγ inhibitor, was found significantly to combat the effects of 1.0 μmol/L Roxarsone on the ECs. Roxarsone is capable of promoting the growth of mouse B16-F10 xenografts and tube formation in vascular ECs. Moreover, VEGFR2/PLCγ/PKC signaling may play a regulatory role in in vivo and in vitro Roxarsone-induced angiogenesis.
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Tumor-promoting and pro-angiogenic effects of Roxarsone via VEGFR2/PLCγ/PKC signaling.
Chemico-biological interactions, 2018Co-Authors: Yanhua Pang, Yujing Wang, Kai Wang, Zhongri Chenlin, Weiwei Lei, Yumei ZhangAbstract:Abstract Roxarsone is an organoarsenic feed additive used in livestock and poultry production that is released into the environment, where it poses a risk to human health. It is known to have a tumor-promoting effect that is brought about by pro-angiogenic factors such as vascular endothelial growth factor (VEGF) and it receptors (VEGFR). However, little information is available about the other signaling molecules that could be involved. This study aims to investigate the role of PLCγ/PKC signaling in Roxarsone-induced angiogenesis in a mouse B16-F10 melanoma xenograft model and rat vascular endothelial cells (ECs). Results showed treatment with 5 mg/kg and 25 mg/kg Roxarsone resulted in an obvious increase in the weight and volume of B16-F10 xenografts and PLCγ/PKC phosphorylation in a dose-dependent manner in C57BL/6 mice. SU5416, a VEGFR2 inhibitor, significantly attenuated the tumor growth induced by Roxarsone. Further, 1.0 μmol/L Roxarsone treatment in rat ECs was observed to significantly increase the optical density rate in the MTT assay, the number of BrdU-positive cells in the proliferation assay, the migration distance in the scratch test, and the number of meshes formed in the tube formation assay. In addition, treatment with 1.0 μmol/L Roxarsone was associated with significantly higher phosphorylation of PLCγ/PKC than the control treatment. U73122, a PLCγ inhibitor, was found significantly to combat the effects of 1.0 μmol/L Roxarsone on the ECs. Roxarsone is capable of promoting the growth of mouse B16-F10 xenografts and tube formation in vascular ECs. Moreover, VEGFR2/PLCγ/PKC signaling may play a regulatory role in in vivo and in vitro Roxarsone-induced angiogenesis.
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Roxarsone induces angiogenesis via PI3K/Akt signaling.
Cell & bioscience, 2016Co-Authors: Yujing Wang, Kai Wang, Donglai Yin, Lingmin Zheng, Yumei ZhangAbstract:Background 3-Nitro-4-hydroxy phenyl arsenic acid, Roxarsone, is widely used as an organic arsenic feed additive for livestock and poultry, which may increase the level of arsenic in the environment and the risk of exposure to arsenic in human. Little information is focused on the angiogenesis Roxarsone-induced and its mechanism at present. This paper aims to study the role of PI3K/Akt signaling in Roxarsone-induced angiogenesis in rat vascular endothelial cells and a mouse B16–F10 melanoma xenograft model.
Guowei Chen - One of the best experts on this subject based on the ideXlab platform.
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Molecular density regulating electron transfer efficiency of S. oneidensis MR-1 mediated Roxarsone biotransformation.
Environmental pollution (Barking Essex : 1987), 2020Co-Authors: Gang Wang, Li Liu, Neng Han, Guowei ChenAbstract:Abstract Efficient extracellular electron transport is a key for sufficient bioremediation of organoarsenic pollutants such as 4-hydroxy-3-nitrobenzenearsonic acid (Roxarsone). The related apparent kinetics characteristics are essential for engineering practice of bioremediation activities and for full understanding the environmental fate of Roxarsone, yet remains poorly understood. We report, to our knowledge, the first study of the electron transfer characteristics between Roxarsone and participating S. oneidensis MR-1. The electron transfer rate during Roxarsone biotransformation was estimated up to 3.1 × 106 electrons/cell/s, with its value being clearly associated with the apparent Roxarsone concentration. Lowing Roxarsone concentration extended the average separation distance between cells and neighboring Roxarsone molecules and thereby augmented electric resistance as well as extended cell movement for foraging, thus reduced electron transfer rate. In addition, the presence of Roxarsone significantly stimulated population growth of S. oneidensis MR-1 with nearly doubled maximum specific growth rate, albeit with clearly increased lag time, as compared with that of none-Roxarsone scenario. These findings provide, at the first time, basic biostoichiometry of S. oneidensis MR-1 induced Roxarsone biotransformation, which may shed lights for full understanding of Roxarsone transformation process in waste treatment systems that are necessary for engineering practice and/or environmental risks assessment.
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Limited carbon source retards inorganic arsenic release during Roxarsone degradation in Shewanella oneidensis microbial fuel cells
Applied microbiology and biotechnology, 2018Co-Authors: Guowei Chen, Li Liu, Guoqing Wang, Huasheng Shi, Gang WangAbstract:Directly relevant to the toxicity, mobility, and fate of arsenic, the biotransformation of inorganic and organic arsenicals has been extensively concerned, including Roxarsone, a widely applied organoarsenical feed additive in poultry industry. Yet, little is known about the transformation details of Roxarsone in microbial fuel cells (MFC). In this study, a two-chambered Shewanella oneidensis MR-1 microbial fuel cell was employed to investigate the transformation processes of Roxarsone at various carbon source levels. Results show that limited carbon source remarkably inhibited inorganic arsenic release along Roxarsone transformation, whereas numerous arsenical species were detected to be released into systems with sufficient carbon source supply, including trivalent and pentavalent inorganic arsenics, monomethylarsonous acid (MMA), and 4-hydroxy-3-aminobenzene arsonic acid (HAPA). Shewanella oneidensis MR-1 was able to cleave the C-As bond of trivalent HAPA yielding inorganic arsenics and MMA, even in the absence of the arsI gene encoding ArsI C-As lyase. We proposed a two-step nitro- and pentavalent-arsenate group reduction pathway for the Roxarsone bioelectrochemical transformation. In addition, results indicated that the attached cells onto the electrode surface played a key function in the two-step reduction of Roxarsone to trivalent HAPA, whereas planktonic cells were most likely responsible for the C-As bond breakage and the following dearylation. With these qualitative and quantitative estimations, it provides new insights into the mechanistic understanding of the Roxarsone biotransformation process in microbial fuel cells, which is important for the biogeochemical cycling of arsenic.
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Roxarsone exposure jeopardizes nitrogen removal and regulates bacterial community in biological sequential batch reactors.
Ecotoxicology and environmental safety, 2018Co-Authors: Guowei Chen, Li Liu, Huan Liu, Wei Zhang, Gang WangAbstract:Abstract Roxarsone is widely present in wastewaters of many animal farms in China. However, little is known about how long-term Roxarsone exposure influences the nitrogen removal of biological wastewater treatment in agricultural settings. Here we investigated the nitrogen removal performance of a biological sequential batch reactor (SBR) and the changes of bacterial community, upon long-term Roxarsone exposure. The long-term Roxarsone dosing decreased the SBR nitrogen removal by 52.4%, with an immediate inhibition on denitrification and a delayed inhibition on nitrification. The analyses of bacterial enzymatic activities and 16 S rRNA sequencing revealed that bacterial activities generally decreased, and the nitrogen-cycling bacterial community was changed, particularly by the decrease (Acinetobacter and Methylophilaceae), persistence (Flavobacterium and Methylotenera), and emergence (Aeromonas) of certain bacterial genera. Overall, chronic Roxarsone exposure could suppress nitrification and denitrification, which may even have broad implications on the use efficiency and cycling of nitrogen in agroecosystems.
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shewanella oneidensis mr 1 induced fe iii reduction facilitates Roxarsone transformation
PLOS ONE, 2016Co-Authors: Guowei Chen, Zhengchen Ke, Tengfang Liang, Gang WangAbstract:Although microbial activity and associated iron (oxy)hydroxides are known in general to affect the environmental dynamics of 4-hydroxy-3-nitrobenzenearsonic acid (Roxarsone), the mechanistic understanding of the underlying biophysico-chemical processes remains unclear due to limited experimental information. We studied how Shewanella oneidensis MR-1 –a widely distributed metal-reducing bacterium, in the presence of dissolved Fe(III), affects Roxarsone transformations and biogeochemical cycling in a model aqueous system. The results showed that the MR-1 strain was able to anaerobically use Roxarsone as a terminal electron acceptor and to convert it to a single product, 3-amino-4-hydroxybenzene arsonic acid (AHBAA). The presence of Fe(III) stimulated Roxarsone transformation via MR-1-induced Fe(III) reduction, whereby the resulting Fe(II) acted as an efficient reductant for Roxarsone transformation. In addition, the subsequent secondary Fe(III)/Fe(II) mineralization created conditions for adsorption of organoarsenic compounds to the yielded precipitates and thereby led to arsenic immobilization. The study provided direct evidence of Shewanella oneidensis MR-1-induced direct and Fe(II)-associated Roxarsone transformation. Quantitative estimations revealed a candidate mechanism for the early-stage environmental dynamics of Roxarsone in nature, which is essential for understanding the environmental dynamics of Roxarsone and successful risk assessment.
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Roxarsone, AHBAA and Fe(II) concentration profiles for Fe(II)-induced Roxarsone reduction.
2016Co-Authors: Guowei Chen, Tengfang Liang, Li Liu, Gang WangAbstract:The average concentration (mean ± s.d., n = 3) of Roxarsone, AHBAA and aqueous Fe(II) for the scenario of Fe(II)-induced Roxarsone reduction.
Yujing Wang - One of the best experts on this subject based on the ideXlab platform.
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tumor promoting and pro angiogenic effects of Roxarsone via vegfr2 plcγ pkc signaling
Chemico-Biological Interactions, 2018Co-Authors: Yanhua Pang, Yujing Wang, Kai Wang, Zhongri Chenlin, Weiwei Lei, Yumei ZhangAbstract:Abstract Roxarsone is an organoarsenic feed additive used in livestock and poultry production that is released into the environment, where it poses a risk to human health. It is known to have a tumor-promoting effect that is brought about by pro-angiogenic factors such as vascular endothelial growth factor (VEGF) and it receptors (VEGFR). However, little information is available about the other signaling molecules that could be involved. This study aims to investigate the role of PLCγ/PKC signaling in Roxarsone-induced angiogenesis in a mouse B16-F10 melanoma xenograft model and rat vascular endothelial cells (ECs). Results showed treatment with 5 mg/kg and 25 mg/kg Roxarsone resulted in an obvious increase in the weight and volume of B16-F10 xenografts and PLCγ/PKC phosphorylation in a dose-dependent manner in C57BL/6 mice. SU5416, a VEGFR2 inhibitor, significantly attenuated the tumor growth induced by Roxarsone. Further, 1.0 μmol/L Roxarsone treatment in rat ECs was observed to significantly increase the optical density rate in the MTT assay, the number of BrdU-positive cells in the proliferation assay, the migration distance in the scratch test, and the number of meshes formed in the tube formation assay. In addition, treatment with 1.0 μmol/L Roxarsone was associated with significantly higher phosphorylation of PLCγ/PKC than the control treatment. U73122, a PLCγ inhibitor, was found significantly to combat the effects of 1.0 μmol/L Roxarsone on the ECs. Roxarsone is capable of promoting the growth of mouse B16-F10 xenografts and tube formation in vascular ECs. Moreover, VEGFR2/PLCγ/PKC signaling may play a regulatory role in in vivo and in vitro Roxarsone-induced angiogenesis.
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Tumor-promoting and pro-angiogenic effects of Roxarsone via VEGFR2/PLCγ/PKC signaling.
Chemico-biological interactions, 2018Co-Authors: Yanhua Pang, Yujing Wang, Kai Wang, Zhongri Chenlin, Weiwei Lei, Yumei ZhangAbstract:Abstract Roxarsone is an organoarsenic feed additive used in livestock and poultry production that is released into the environment, where it poses a risk to human health. It is known to have a tumor-promoting effect that is brought about by pro-angiogenic factors such as vascular endothelial growth factor (VEGF) and it receptors (VEGFR). However, little information is available about the other signaling molecules that could be involved. This study aims to investigate the role of PLCγ/PKC signaling in Roxarsone-induced angiogenesis in a mouse B16-F10 melanoma xenograft model and rat vascular endothelial cells (ECs). Results showed treatment with 5 mg/kg and 25 mg/kg Roxarsone resulted in an obvious increase in the weight and volume of B16-F10 xenografts and PLCγ/PKC phosphorylation in a dose-dependent manner in C57BL/6 mice. SU5416, a VEGFR2 inhibitor, significantly attenuated the tumor growth induced by Roxarsone. Further, 1.0 μmol/L Roxarsone treatment in rat ECs was observed to significantly increase the optical density rate in the MTT assay, the number of BrdU-positive cells in the proliferation assay, the migration distance in the scratch test, and the number of meshes formed in the tube formation assay. In addition, treatment with 1.0 μmol/L Roxarsone was associated with significantly higher phosphorylation of PLCγ/PKC than the control treatment. U73122, a PLCγ inhibitor, was found significantly to combat the effects of 1.0 μmol/L Roxarsone on the ECs. Roxarsone is capable of promoting the growth of mouse B16-F10 xenografts and tube formation in vascular ECs. Moreover, VEGFR2/PLCγ/PKC signaling may play a regulatory role in in vivo and in vitro Roxarsone-induced angiogenesis.
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Roxarsone induces angiogenesis via PI3K/Akt signaling.
Cell & bioscience, 2016Co-Authors: Yujing Wang, Kai Wang, Donglai Yin, Lingmin Zheng, Yumei ZhangAbstract:Background 3-Nitro-4-hydroxy phenyl arsenic acid, Roxarsone, is widely used as an organic arsenic feed additive for livestock and poultry, which may increase the level of arsenic in the environment and the risk of exposure to arsenic in human. Little information is focused on the angiogenesis Roxarsone-induced and its mechanism at present. This paper aims to study the role of PI3K/Akt signaling in Roxarsone-induced angiogenesis in rat vascular endothelial cells and a mouse B16–F10 melanoma xenograft model.
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Roxarsone induces angiogenesis via pi3k akt signaling
Cell & Bioscience, 2016Co-Authors: Yujing Wang, Kai Wang, Donglai Yin, Lingmin Zheng, Yumei ZhangAbstract:Background 3-Nitro-4-hydroxy phenyl arsenic acid, Roxarsone, is widely used as an organic arsenic feed additive for livestock and poultry, which may increase the level of arsenic in the environment and the risk of exposure to arsenic in human. Little information is focused on the angiogenesis Roxarsone-induced and its mechanism at present. This paper aims to study the role of PI3K/Akt signaling in Roxarsone-induced angiogenesis in rat vascular endothelial cells and a mouse B16–F10 melanoma xenograft model.
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Organoarsenic Roxarsone Promotes Angiogenesis In Vivo
Basic & clinical pharmacology & toxicology, 2015Co-Authors: Yumei Zhang, Yujing Wang, Wenfang Xin, Weibo Cui, Jiaqiao ZhuAbstract:Roxarsone, an organoarsenic feed additive, is widely used worldwide to promote animal growth. It has been found to exhibit a higher angiogenic index than As(III) at lower concentrations and to promote angiogenic phenotype in human endothelial cell in vitro. Little research has focused on the potential angiogenic effect of Roxarsone in vitro or in vivo. Here, we investigated the pro-angiogenic effect of Roxarsone in vivo. The effects of 0.1-10.0 μM Roxarsone were tested in the rat endothelial cell Matrigel plug assay, chicken chorioallantoic membrane (CAM) model and MCF-7 cell xenograft tumour model; 10 ng/mL vascular endothelial growth factor (VEGF) was used as a positive control and PBS as a negative control. Roxarsone significantly increased the volume, weight and haemoglobin content of the Matrigel plugs compared to PBS group (p < 0.05); 1.0 μM Roxarsone exerted the most significant effects. H&E staining and CD31 immunochemistry revealed obviously more new vessels or capillary-like structures in the plugs of the Roxarsone and VEGF groups. Roxarsone significantly increased the numbers of primary/secondary vessels and area of vessels in the CAM assay and obviously increased tumour weight and volume in the xenograft model compared to PBS (p < 0.05). Histochemistry indicated local necrosis was observed at the centre of the xenograft tumours in the PBS and Roxarsone groups, with less necrosis apparent in the VEGF-treated tumours. The growth of endothelial cells and VEGF level was obviously affected at blockade of VEGF and its receptor Flt-1/Flk-1 by SU5416 or its antibody in vitro. This study demonstrates Roxarsone promotes angiogenesis in vivo, and a VEGF/VEGFR mechanism may be involved.
Shoujun Yuan - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Roxarsone photocatalytic degradation and arsenic adsorption removal by TiO2/FeOOH hybrid.
Environmental science and pollution research international, 2020Co-Authors: Hang Yao, Shoujun Yuan, Wei Wang, Miao GongAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) is an extensively used organoarsenic feed additive. The effective removal of arsenic from Roxarsone degradation before discharging is of great importance for controlling artificial arsenic pollution in aquatic environment. In this study, a bifunctional TiO2/ferrihydrite (TiO2/FeOOH) hybrid was synthesized by a hydrothermal method for the simultaneously photocatalytic degradation of Roxarsone and adsorption removal of released arsenic. The analysis of the prepared TiO2/FeOOH by field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), Raman spectra, X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the hybrid of crystalline TiO2 and no crystalline FeOOH. TiO2/FeOOH hybrid had better adsorption capacity for As(V) than Roxarsone. Compared to TiO2, the TiO2/FeOOH hybrid exhibited much superior UV-driven photocatalytic activities for Roxarsone degradation. After 12 h irradiation, more than 96% of Roxarsone was degraded by 1:1 TiO2/FeOOH hybrid, and the released As(V) was simultaneously removed from the solution. The residual As(V) concentration was lower than 0.02 mg L−1. The reusability test indicated that TiO2/FeOOH hybrid had excellent stability and reliability. The possible mechanism of Roxarsone degradation and released inorganic arsenics removal by this hybrid was also proposed. These results clearly indicated that the TiO2/FeOOH hybrid could be used for the removal of Roxarsone and its degradation product.
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simultaneous Roxarsone photocatalytic degradation and arsenic adsorption removal by tio2 feooh hybrid
Environmental Science and Pollution Research, 2020Co-Authors: Hang Yao, Shoujun Yuan, Wei Wang, Miao GongAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) is an extensively used organoarsenic feed additive. The effective removal of arsenic from Roxarsone degradation before discharging is of great importance for controlling artificial arsenic pollution in aquatic environment. In this study, a bifunctional TiO2/ferrihydrite (TiO2/FeOOH) hybrid was synthesized by a hydrothermal method for the simultaneously photocatalytic degradation of Roxarsone and adsorption removal of released arsenic. The analysis of the prepared TiO2/FeOOH by field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), Raman spectra, X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the hybrid of crystalline TiO2 and no crystalline FeOOH. TiO2/FeOOH hybrid had better adsorption capacity for As(V) than Roxarsone. Compared to TiO2, the TiO2/FeOOH hybrid exhibited much superior UV-driven photocatalytic activities for Roxarsone degradation. After 12 h irradiation, more than 96% of Roxarsone was degraded by 1:1 TiO2/FeOOH hybrid, and the released As(V) was simultaneously removed from the solution. The residual As(V) concentration was lower than 0.02 mg L−1. The reusability test indicated that TiO2/FeOOH hybrid had excellent stability and reliability. The possible mechanism of Roxarsone degradation and released inorganic arsenics removal by this hybrid was also proposed. These results clearly indicated that the TiO2/FeOOH hybrid could be used for the removal of Roxarsone and its degradation product.
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Effects of Roxarsone and sulfadiazine on biogas production and their degradation during anaerobic digestion
International Biodeterioration & Biodegradation, 2019Co-Authors: Rui Tang, Shoujun Yuan, Fengqin Chen, Xinmin Zhan, Wei WangAbstract:Abstract Roxarsone and sulfadiazine are typical feed additives and often occur in manure and livestock wastewater. Anaerobic digestion is a common technique for the treatment of manure and livestock wastewater. However, the influence of Roxarsone and sulfadiazine on anaerobic digestion and their degradation are still unclear. In this study, the effect of Roxarsone and sulfadiazine on the anaerobic digestion of synthetic wastewater and their degradation were investigated. During 70-day incubation, the presence of 200 mg L−1 sulfadiazine exhibited little influence on hydrolysis and acidification, but resulted in a temporary inhibition on methanogenesis, whereas 120 mg L−1 of Roxarsone resulted in nearly complete inhibition on methanogenesis. Further, the co-existence of 100 mg L−1 sulfadiazine and 60 mg L−1 Roxarsone significantly exacerbated the inhibition on anaerobic digestion. Sulfadiazine exhibited high resistance to the adsorption and biodegradation, while Roxarsone was nearly completely degraded during anaerobic digestion. Nearly half of total arsenic was transferred from solution to anaerobic granular sludge. The predominant arsenic species was 4-hydroxy-3-aminophenylarsonic acid in the solution and As3+ in the sludge. These results will provide valuable information for anaerobic treatment of livestock wastewater simultaneously contaminated by Roxarsone and sulfadiazine.
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Biodegradation and speciation of Roxarsone in an anaerobic granular sludge system and its impacts.
Journal of hazardous materials, 2014Co-Authors: Fei-fei Zhang, Wei Wang, Shoujun YuanAbstract:Abstract Roxarsone (3-nitro-4-hydroxy benzene arsenic acid) is an organoarsenic feed additive and has been widely used in the poultry industry to prevent coccidiosis and improve feed efficiency. The presence of Roxarsone and its degradation products results in the instability of the anaerobic methanogenic process. This study investigated the degradation and speciation of Roxarsone in an anaerobic granular sludge (AGS) system and the impacts of Roxarsone and its degradation products on the structure of AGS. Roxarsone inhibited methane production, and the added Roxarsone was rapidly degraded into 3-amino-4-hydroxyphenylarsonic acid (HAPA). After 240 days of incubation, the distribution of arsenic differed between the aqueous solution and the AGS in the assays of 20 and 350 mg/L Roxarsone. Species analysis indicated that HAPA was completely degraded in all of the assays with Roxarsone addition after 240 days of incubation. Species distribution was affected by the phases and the initial concentration of Roxarsone added. The concentration of As(III) was higher than that of As(V) in both the aqueous solution and the AGS in all assays with Roxarsone addition. The toxicity of Roxarsone and its degradation products resulted in changes in the structure and the microorganism species in the AGS.
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Adsorption and photocatalytic decomposition of Roxarsone by TiO2 and its mechanism
Environmental Science and Pollution Research, 2014Co-Authors: Donglei Lu, Zhen-hu Hu, Shoujun Yuan, Feng Ji, Wei Wang, Tianhu ChenAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) has been widely used as organic arsenic additive in animal industry. In this study, the adsorption of Roxarsone on TiO2 under dark conditions, the photocatalytic decomposition of Roxarsone under UV/TiO2, and the possible photocatalytic pathway were investigated. At the initial concentration of 5–35 mg/L, the adsorption of Roxarsone fitted well with the pseudo-second-order kinetics. The isotherms analysis showed that the Langmuir model was better than the Freundlich and Dubinin–Radushkevich models for describing the adsorption process. After 7 h of photocatalytic decomposition, a complete disappearance of Roxarsone was achieved. The pH value has a significant effect on both adsorption and photocatalytic decomposition of Roxarsone. The results of high-performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) and gas chromatography-mass spectrometry (GC/MS) analyses proved the cleavage of the As-C bond during the photocatalytic decomposition process by TiO2 and the intermediates of the decomposition. Based on the results, a possible photocatalytic decomposition pathway was proposed.
Wei Wang - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Roxarsone photocatalytic degradation and arsenic adsorption removal by TiO2/FeOOH hybrid.
Environmental science and pollution research international, 2020Co-Authors: Hang Yao, Shoujun Yuan, Wei Wang, Miao GongAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) is an extensively used organoarsenic feed additive. The effective removal of arsenic from Roxarsone degradation before discharging is of great importance for controlling artificial arsenic pollution in aquatic environment. In this study, a bifunctional TiO2/ferrihydrite (TiO2/FeOOH) hybrid was synthesized by a hydrothermal method for the simultaneously photocatalytic degradation of Roxarsone and adsorption removal of released arsenic. The analysis of the prepared TiO2/FeOOH by field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), Raman spectra, X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the hybrid of crystalline TiO2 and no crystalline FeOOH. TiO2/FeOOH hybrid had better adsorption capacity for As(V) than Roxarsone. Compared to TiO2, the TiO2/FeOOH hybrid exhibited much superior UV-driven photocatalytic activities for Roxarsone degradation. After 12 h irradiation, more than 96% of Roxarsone was degraded by 1:1 TiO2/FeOOH hybrid, and the released As(V) was simultaneously removed from the solution. The residual As(V) concentration was lower than 0.02 mg L−1. The reusability test indicated that TiO2/FeOOH hybrid had excellent stability and reliability. The possible mechanism of Roxarsone degradation and released inorganic arsenics removal by this hybrid was also proposed. These results clearly indicated that the TiO2/FeOOH hybrid could be used for the removal of Roxarsone and its degradation product.
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simultaneous Roxarsone photocatalytic degradation and arsenic adsorption removal by tio2 feooh hybrid
Environmental Science and Pollution Research, 2020Co-Authors: Hang Yao, Shoujun Yuan, Wei Wang, Miao GongAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) is an extensively used organoarsenic feed additive. The effective removal of arsenic from Roxarsone degradation before discharging is of great importance for controlling artificial arsenic pollution in aquatic environment. In this study, a bifunctional TiO2/ferrihydrite (TiO2/FeOOH) hybrid was synthesized by a hydrothermal method for the simultaneously photocatalytic degradation of Roxarsone and adsorption removal of released arsenic. The analysis of the prepared TiO2/FeOOH by field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), Raman spectra, X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), and X-ray photoelectron spectroscopy (XPS) confirmed the successful formation of the hybrid of crystalline TiO2 and no crystalline FeOOH. TiO2/FeOOH hybrid had better adsorption capacity for As(V) than Roxarsone. Compared to TiO2, the TiO2/FeOOH hybrid exhibited much superior UV-driven photocatalytic activities for Roxarsone degradation. After 12 h irradiation, more than 96% of Roxarsone was degraded by 1:1 TiO2/FeOOH hybrid, and the released As(V) was simultaneously removed from the solution. The residual As(V) concentration was lower than 0.02 mg L−1. The reusability test indicated that TiO2/FeOOH hybrid had excellent stability and reliability. The possible mechanism of Roxarsone degradation and released inorganic arsenics removal by this hybrid was also proposed. These results clearly indicated that the TiO2/FeOOH hybrid could be used for the removal of Roxarsone and its degradation product.
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Effects of Roxarsone and sulfadiazine on biogas production and their degradation during anaerobic digestion
International Biodeterioration & Biodegradation, 2019Co-Authors: Rui Tang, Shoujun Yuan, Fengqin Chen, Xinmin Zhan, Wei WangAbstract:Abstract Roxarsone and sulfadiazine are typical feed additives and often occur in manure and livestock wastewater. Anaerobic digestion is a common technique for the treatment of manure and livestock wastewater. However, the influence of Roxarsone and sulfadiazine on anaerobic digestion and their degradation are still unclear. In this study, the effect of Roxarsone and sulfadiazine on the anaerobic digestion of synthetic wastewater and their degradation were investigated. During 70-day incubation, the presence of 200 mg L−1 sulfadiazine exhibited little influence on hydrolysis and acidification, but resulted in a temporary inhibition on methanogenesis, whereas 120 mg L−1 of Roxarsone resulted in nearly complete inhibition on methanogenesis. Further, the co-existence of 100 mg L−1 sulfadiazine and 60 mg L−1 Roxarsone significantly exacerbated the inhibition on anaerobic digestion. Sulfadiazine exhibited high resistance to the adsorption and biodegradation, while Roxarsone was nearly completely degraded during anaerobic digestion. Nearly half of total arsenic was transferred from solution to anaerobic granular sludge. The predominant arsenic species was 4-hydroxy-3-aminophenylarsonic acid in the solution and As3+ in the sludge. These results will provide valuable information for anaerobic treatment of livestock wastewater simultaneously contaminated by Roxarsone and sulfadiazine.
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Biodegradation and speciation of Roxarsone in an anaerobic granular sludge system and its impacts.
Journal of hazardous materials, 2014Co-Authors: Fei-fei Zhang, Wei Wang, Shoujun YuanAbstract:Abstract Roxarsone (3-nitro-4-hydroxy benzene arsenic acid) is an organoarsenic feed additive and has been widely used in the poultry industry to prevent coccidiosis and improve feed efficiency. The presence of Roxarsone and its degradation products results in the instability of the anaerobic methanogenic process. This study investigated the degradation and speciation of Roxarsone in an anaerobic granular sludge (AGS) system and the impacts of Roxarsone and its degradation products on the structure of AGS. Roxarsone inhibited methane production, and the added Roxarsone was rapidly degraded into 3-amino-4-hydroxyphenylarsonic acid (HAPA). After 240 days of incubation, the distribution of arsenic differed between the aqueous solution and the AGS in the assays of 20 and 350 mg/L Roxarsone. Species analysis indicated that HAPA was completely degraded in all of the assays with Roxarsone addition after 240 days of incubation. Species distribution was affected by the phases and the initial concentration of Roxarsone added. The concentration of As(III) was higher than that of As(V) in both the aqueous solution and the AGS in all assays with Roxarsone addition. The toxicity of Roxarsone and its degradation products resulted in changes in the structure and the microorganism species in the AGS.
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Adsorption and photocatalytic decomposition of Roxarsone by TiO2 and its mechanism
Environmental Science and Pollution Research, 2014Co-Authors: Donglei Lu, Zhen-hu Hu, Shoujun Yuan, Feng Ji, Wei Wang, Tianhu ChenAbstract:Roxarsone (3-nitro-4-hydroxyphenylarsonic acid) has been widely used as organic arsenic additive in animal industry. In this study, the adsorption of Roxarsone on TiO2 under dark conditions, the photocatalytic decomposition of Roxarsone under UV/TiO2, and the possible photocatalytic pathway were investigated. At the initial concentration of 5–35 mg/L, the adsorption of Roxarsone fitted well with the pseudo-second-order kinetics. The isotherms analysis showed that the Langmuir model was better than the Freundlich and Dubinin–Radushkevich models for describing the adsorption process. After 7 h of photocatalytic decomposition, a complete disappearance of Roxarsone was achieved. The pH value has a significant effect on both adsorption and photocatalytic decomposition of Roxarsone. The results of high-performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) and gas chromatography-mass spectrometry (GC/MS) analyses proved the cleavage of the As-C bond during the photocatalytic decomposition process by TiO2 and the intermediates of the decomposition. Based on the results, a possible photocatalytic decomposition pathway was proposed.
