The Experts below are selected from a list of 621 Experts worldwide ranked by ideXlab platform
Xuebo Liu - One of the best experts on this subject based on the ideXlab platform.
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Sesamol supplementation attenuates dss induced colitis via mediating gut barrier integrity inflammatory responses and reshaping gut microbiome
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Beita Zhao, Zhigang Liu, Bing Xia, Li Zhang, Xiaoning Liu, Renjie Shi, Rongwei Kou, Xuebo LiuAbstract:Sesamol, a liposoluble lignan extract, has already been proved to possess potent anti-inflammatory properties, and it could also regulate gut dysfunction. The purpose of the present research is to explore the protective effect of Sesamol on colitis mice. In the current research, Sesamol treatment (100 mg/kg bodyweight/day) for 6 weeks inhibited the dextran sulphate sodium (DSS)-induced bodyweight loss of mice. Transmission electron microscopy and hematoxylin and eosin staining results showed that the DSS-induced histopathological changes of mice were also recovered by Sesamol supplementation. In addition, DSS-induced inflammatory responses were inhibited by Sesamol supplementation via the NF-κB signaling pathway in mice colon. Moreover, Sesamol treatment prevented gut barrier damages by enhancing the expression of tight junction proteins (occludin, claudin-1, and ZO-1) and recovering the loss of gut mucus layer. Furthermore, Sesamol supplementation also increased the short-chain fatty acid (SCFAs) contents of acetate, propionate, and butyrate. Furthermore, Sesamol supplementation changed the gut microbiome structure by enhancing the relative abundance of Coprococcuscus, Butyricicoccus, Odoribacter, and AF12 in colitis mice. In conclusion, Sesamol could effectively ameliorate DSS-induced colitis by promoting gut microecology.
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Sesamol incorporated cellulose acetate zein composite nanofiber membrane an efficient strategy to accelerate diabetic wound healing
International Journal of Biological Macromolecules, 2020Co-Authors: Fuguo Liu, Zhigang Liu, Lin Wang, Xiaojia Yan, Xuebo LiuAbstract:Recently, the function of nanofiber membranes prepared from electrospinning in accelerating wound healing has attracted wide attention. In this study, novel nanofiber membranes consisted of cellulose acetate (CA) and zein were fabricated to provide efficient delivery vehicles for Sesamol, and then the effect of Sesamol-loaded composite nanofiber membranes on the wound healing of diabetic mice was studied. It was found the critical concentration of CA was between 15% and 25% (w/v), and the most suitable concentration of stabilizing fibers was 22.5%. When the CA/zein ratio was 12:8, the fiber obtained small diameter and uniform distribution, stable intermolecular structure, low infiltration speed and high stability in water. The composite nanofiber membrane with high-dose Sesamol (5% of total polymer concentration, w/w) promoted formation of myofibroblasts by enhancing TGF-β signaling pathway transduction, and promoted keratinocyte growth by inhibiting chronic inflammation in wounds, thus enhancing wound healing in diabetic mice. This study can further broaden the application range of Sesamol, CA and zein, and provide reference for the design and development of new wound dressings in the future.
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delivery of Sesamol using polyethylene glycol functionalized selenium nanoparticles in human liver cells in culture
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Fuguo Liu, Hua Liu, Runhua Liu, Chunxia Xiao, Xiang Duan, David Julian Mcclements, Xuebo LiuAbstract:Anticancer nanoparticles were fabricated by linking the nanoparticles of two known anticancer agents, Sesamol and selenium, using polyethylene glycol (PEG). The successful fabrication of the Sesamol-PEG-selenium nanoparticles (PEG-SeNPs), which had a Sesamol loading efficiency of 10.0 ± 0.5 wt %, was demonstrated using different spectroscopic techniques. The impact of the nanoparticles on model cancer cells (HepG2) was established using the cell activity test, morphological observation, and fluorescent staining, which all showed that nanoparticles effectively inhibited the HepG2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that the concentration of the sample that inhibits 50% of the cells of PEG-SeNPs and Sesamol-PEG-SeNPs on HepG2 cells was 413.8 and 68.7 μg/mL, respectively, which indicated the synergistic inhibition between Sesamol and selenium nanoparticles. Furthermore, flow cytometry showed that Sesamol-PEG-SeNPs exhibited higher apoptosis than either Sesamol or PEG-SeNPs alone. Finally, western blot confirmed that the apoptostic ability of Sesamol-PEG-SeNPs was associated with downregulation of Bcl-2 and procaspase-3, upregulation of Bax and PARP, and discharge of cytochrome c into the cytosol. Our findings suggest the novel Sesamol nanoparticles may be efficient anticancer agents.
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protective effects of Sesamol on systemic oxidative stress induced cognitive impairments via regulation of nrf2 keap1 pathway
Food & Function, 2018Co-Authors: Bo Ren, Tian Yuan, Zhigang Liu, Zhijun Diao, Chenxi Zhang, Xuebo LiuAbstract:Oxidative stress is considered as a pivotal culprit in neurodegenerative diseases and brain aging. The aim of present study was to investigate antioxidative and neuroprotective effects of Sesamol, a phenolic lignan from sesame oil, on oxidative stress induced neuron damage and memory impairments. C57BL/6J mice were treated by intraperitoneal injections of D-galactose for 8 weeks. Sesamol treatment (0.05% w/v, in drinking water) suppressed D-galactose-induced liver damages and improved HO-1 and NQO1 mRNA levels. Behavioral tests, including Y-maze test and water maze-test, revealed that Sesamol significantly improved oxidative stress-induced cognitive impairments. Meanwhile, Sesamol ameliorated neuronal damage and improved BDNF level in rat hippocampus. Sesamol elevated mRNA levels and protein expressions of antioxidant enzymes HO-1 and NQO1 as well as decreased inflammatory cytokines TNF-α and IL-1β in D-galactose-treated mice serum. In addition, activity of CAT and GSH level were increased in Sesamol-treated mice serum. Moreover, Sesamol treatment also balanced cellular redox status, protected mitochondrial dysfunction and upregulated antioxidant enzymes by activating the Nrf2 transcriptional pathway and its nuclear translocation in H2O2-treated SH-SY5Y cells. In conclusion, these results revealed that Sesamol could be a potential neuroprotective agent during aging process due to its beneficial effects on liver–brain axis.
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extract of sesame cake and Sesamol alleviate chronic unpredictable mild stress induced depressive like behaviors and memory deficits
Journal of Functional Foods, 2018Co-Authors: Zhigang Liu, Chuanqi Chu, Xiaoning Liu, Runhua Liu, Shunyi Luo, Lei Wang, Jiamin Wang, Xuebo LiuAbstract:Abstract Depression is a worldwide severe psychiatric disease associated with cognitive impairments. The aims of the present study are to investigate the preventive effects of alcoholic extract of sesame (Sesamum indicum L.) cake (SLE) and Sesamol in a chronic unpredictable mild stress (CUMS)-induced mouse model. Oral administration of SLE (600 mg/kg/day) and Sesamol (10 mg/kg/day) significantly restored CUMS-induced mice antidepressant-like behaviors, anhedonia, and anxiety. Importantly, supplementation of SLE and Sesamol inhibited oxidative stress and improved serotonin levels in depressed mice brain. Moreover, SLE and Sesamol treatment significantly prevented CUMS-induced memory loss in Y-maze and water-maze tests, which was consistent with enhanced the size of postsynaptic densities and postsynaptic density protein 95 (PSD-95) expression in mice hippocampus. These results illustrated that SLE and Sesamol markedly improved CUMS-induced depression and memory loss, and provided novel insights into the mechanisms of Sesamol and sesame crude extract on the regulation CUMS-induced depression and cognitive impairments.
Zhigang Liu - One of the best experts on this subject based on the ideXlab platform.
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Sesamol supplementation attenuates dss induced colitis via mediating gut barrier integrity inflammatory responses and reshaping gut microbiome
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Beita Zhao, Zhigang Liu, Bing Xia, Li Zhang, Xiaoning Liu, Renjie Shi, Rongwei Kou, Xuebo LiuAbstract:Sesamol, a liposoluble lignan extract, has already been proved to possess potent anti-inflammatory properties, and it could also regulate gut dysfunction. The purpose of the present research is to explore the protective effect of Sesamol on colitis mice. In the current research, Sesamol treatment (100 mg/kg bodyweight/day) for 6 weeks inhibited the dextran sulphate sodium (DSS)-induced bodyweight loss of mice. Transmission electron microscopy and hematoxylin and eosin staining results showed that the DSS-induced histopathological changes of mice were also recovered by Sesamol supplementation. In addition, DSS-induced inflammatory responses were inhibited by Sesamol supplementation via the NF-κB signaling pathway in mice colon. Moreover, Sesamol treatment prevented gut barrier damages by enhancing the expression of tight junction proteins (occludin, claudin-1, and ZO-1) and recovering the loss of gut mucus layer. Furthermore, Sesamol supplementation also increased the short-chain fatty acid (SCFAs) contents of acetate, propionate, and butyrate. Furthermore, Sesamol supplementation changed the gut microbiome structure by enhancing the relative abundance of Coprococcuscus, Butyricicoccus, Odoribacter, and AF12 in colitis mice. In conclusion, Sesamol could effectively ameliorate DSS-induced colitis by promoting gut microecology.
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Sesamol incorporated cellulose acetate zein composite nanofiber membrane an efficient strategy to accelerate diabetic wound healing
International Journal of Biological Macromolecules, 2020Co-Authors: Fuguo Liu, Zhigang Liu, Lin Wang, Xiaojia Yan, Xuebo LiuAbstract:Recently, the function of nanofiber membranes prepared from electrospinning in accelerating wound healing has attracted wide attention. In this study, novel nanofiber membranes consisted of cellulose acetate (CA) and zein were fabricated to provide efficient delivery vehicles for Sesamol, and then the effect of Sesamol-loaded composite nanofiber membranes on the wound healing of diabetic mice was studied. It was found the critical concentration of CA was between 15% and 25% (w/v), and the most suitable concentration of stabilizing fibers was 22.5%. When the CA/zein ratio was 12:8, the fiber obtained small diameter and uniform distribution, stable intermolecular structure, low infiltration speed and high stability in water. The composite nanofiber membrane with high-dose Sesamol (5% of total polymer concentration, w/w) promoted formation of myofibroblasts by enhancing TGF-β signaling pathway transduction, and promoted keratinocyte growth by inhibiting chronic inflammation in wounds, thus enhancing wound healing in diabetic mice. This study can further broaden the application range of Sesamol, CA and zein, and provide reference for the design and development of new wound dressings in the future.
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protective effects of Sesamol on systemic inflammation and cognitive impairment in aging mice
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Bo Ren, Tian Yuan, Xinglin Zhang, Luanfeng Wang, Junru Pan, Yan Liu, Beita Zhao, Weiyang Zhao, Zhigang LiuAbstract:Sesamol, a lignan in sesame, possesses several bioactivities, such as antioxidation, anti-inflammation, and neuroprotective capability. In this study, the effects of Sesamol on aging-caused cognitive defects are investigated. Twelve-month-old mice were treated with Sesamol (0.1%, w/w) as dietary supplementation for 12 weeks. Behavioral tests revealed that Sesamol improved aging-associated cognitive impairments. Sesamol decreased aging-induced oxidative stress via suppression of malondialdehyde production and increased antioxidant enzymes. Histological staining showed that Sesamol treatment improved aging-induced neuronal damage and synaptic dysfunction in the hippocampus. Furthermore, Sesamol significantly reduced aging-induced neuroinflammation by inhibiting the microglial overactivation and inflammatory cytokine expressions. Meanwhile, the accumulation of Aβ1-42 was reduced by Sesamol treatment. Moreover, Sesamol protected the gut barrier integrity and reduced LPS release, which was highly associated with its beneficial effects on behavioral and inflammatory changes. In conclusion, our findings indicated that the use of Sesamol is feasible in the treatment of aging-related diseases.
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protective effects of Sesamol on systemic oxidative stress induced cognitive impairments via regulation of nrf2 keap1 pathway
Food & Function, 2018Co-Authors: Bo Ren, Tian Yuan, Zhigang Liu, Zhijun Diao, Chenxi Zhang, Xuebo LiuAbstract:Oxidative stress is considered as a pivotal culprit in neurodegenerative diseases and brain aging. The aim of present study was to investigate antioxidative and neuroprotective effects of Sesamol, a phenolic lignan from sesame oil, on oxidative stress induced neuron damage and memory impairments. C57BL/6J mice were treated by intraperitoneal injections of D-galactose for 8 weeks. Sesamol treatment (0.05% w/v, in drinking water) suppressed D-galactose-induced liver damages and improved HO-1 and NQO1 mRNA levels. Behavioral tests, including Y-maze test and water maze-test, revealed that Sesamol significantly improved oxidative stress-induced cognitive impairments. Meanwhile, Sesamol ameliorated neuronal damage and improved BDNF level in rat hippocampus. Sesamol elevated mRNA levels and protein expressions of antioxidant enzymes HO-1 and NQO1 as well as decreased inflammatory cytokines TNF-α and IL-1β in D-galactose-treated mice serum. In addition, activity of CAT and GSH level were increased in Sesamol-treated mice serum. Moreover, Sesamol treatment also balanced cellular redox status, protected mitochondrial dysfunction and upregulated antioxidant enzymes by activating the Nrf2 transcriptional pathway and its nuclear translocation in H2O2-treated SH-SY5Y cells. In conclusion, these results revealed that Sesamol could be a potential neuroprotective agent during aging process due to its beneficial effects on liver–brain axis.
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extract of sesame cake and Sesamol alleviate chronic unpredictable mild stress induced depressive like behaviors and memory deficits
Journal of Functional Foods, 2018Co-Authors: Zhigang Liu, Chuanqi Chu, Xiaoning Liu, Runhua Liu, Shunyi Luo, Lei Wang, Jiamin Wang, Xuebo LiuAbstract:Abstract Depression is a worldwide severe psychiatric disease associated with cognitive impairments. The aims of the present study are to investigate the preventive effects of alcoholic extract of sesame (Sesamum indicum L.) cake (SLE) and Sesamol in a chronic unpredictable mild stress (CUMS)-induced mouse model. Oral administration of SLE (600 mg/kg/day) and Sesamol (10 mg/kg/day) significantly restored CUMS-induced mice antidepressant-like behaviors, anhedonia, and anxiety. Importantly, supplementation of SLE and Sesamol inhibited oxidative stress and improved serotonin levels in depressed mice brain. Moreover, SLE and Sesamol treatment significantly prevented CUMS-induced memory loss in Y-maze and water-maze tests, which was consistent with enhanced the size of postsynaptic densities and postsynaptic density protein 95 (PSD-95) expression in mice hippocampus. These results illustrated that SLE and Sesamol markedly improved CUMS-induced depression and memory loss, and provided novel insights into the mechanisms of Sesamol and sesame crude extract on the regulation CUMS-induced depression and cognitive impairments.
N K Chaudhury - One of the best experts on this subject based on the ideXlab platform.
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Sesamol ameliorates radiation induced dna damage in hematopoietic system of whole body γ irradiated mice
Environmental and Molecular Mutagenesis, 2018Co-Authors: Arun Kumar, Sandeep Choudhary, J S Adhikari, N K ChaudhuryAbstract:Ionizing radiation exposure is harmful and at high doses can lead to acute hematopoietic radiation syndrome. Therefore, agents that can protect hematopoietic system are important for development of radioprotector. Sesamol is a potential molecule for development of radioprotector due to its strong free radical scavenging and antioxidant properties. In the present study, Sesamol was evaluated for its role in DNA damage and repair in hematopoietic system of γ-irradiated CB57BL/6 mice and compared with amifostine. C57BL/6 male mice were administered with Sesamol 20 mg/kg (i.p.) followed by 2 Gy whole body irradiation (WBI) at 30 min. Mice were sacrificed at 0.5, 3, 24 h postirradiation; bone marrow, splenocytes, and peripheral blood lymphocytes were isolated to measure DNA damages and repair using alkaline comet,γ-H2AXand micronucleus assays. An increase in % of tail DNA was observed in all organs of WBI mice. Whereas in pre-administered Sesamol reduced %DNA in tail (P ≤ 0.05). Sesamol has also reduced formation of radiation induced γ-H2AX foci after 0.5 h in these organs and further lowered to respective control values at 24 h of WBI. Similar reduction of % DNA in tail and γ-H2AX foci were observed with amifostine (P ≤ 0.05). Analysis of mnPCE frequency at 24 h has revealed similar extent of protection by Sesamol and amifostine. Interestingly, both Sesamol and amifostine, alone and with radiation, also increased the granulocytes count significantly compared to the control (P ≤ 0.05). These findings suggest that Sesamol has strong potential to protect hematopoietic system by lowering radiation induced DNA damages and can prevent acute hematopoietic syndrome in mice. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc.
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protective effect of Sesamol against co γ ray induced hematopoietic and gastrointestinal injury in c57bl 6 male mice
Free Radical Research, 2015Co-Authors: Shahanshah Khan, J S Adhikari, Arun Kumar, Moshahid Alam M Rizvi, N K ChaudhuryAbstract:Protection of γ-ray-induced injury in hematopoietic and gastrointestinal (GI) systems is the rationale behind developing radioprotectors. The objective of this study, therefore, was to investigate the radioprotective efficacy and mechanisms underlying Sesamol in amelioration of γ-ray-induced hematopoietic and GI injury in mice. C57BL/6 male mice were pre-treated with a single dose (100 or 50 mg/kg, 30 min prior) of Sesamol through the intraperitoneal route and exposed to LD50/30 (7.5 Gy) and sublethal (5 Gy) dose of γ-radiation. Thirty-day survival against 7.5 Gy was monitored. Sesamol (100 mg/kg) pre-treatment reduced radiation-induced mortality and resulted survival of about 100% against 7.5 Gy of γ-irradiation. Whole-body irradiation drastically depleted hematopoietic progenitor stem cells in bone marrow, B cells, T cell subpopulations, and splenocyte proliferation in the spleen on day 4, which were significantly protected in Sesamol pre-treated mice. This was associated with a decrease of radiation-induced micronuclei (MN) and apoptosis in bone marrow and spleen, respectively. Sesamol pre-treatment inhibited lipid peroxidation, translocation of gut bacteria to spleen, liver, and kidney, and enhanced regeneration of crypt cells in the GI system. In addition, Sesamol pre-treatment reduced the radiation-induced pattern of expression of p53 and Bax apoptotic proteins in the bone marrow, spleen, and GI. This reduction in apoptotic proteins was associated with the increased anti-apoptotic-Bcl-x and PCNA proteins. Further, assessment of antioxidant capacity using ABTS and DPPH assays revealed that Sesamol treatment alleviated total antioxidant capacity in spleen and GI tissue. In conclusion, the results of the present study suggested that Sesamol as a single prophylactic dose protects hematopoietic and GI systems against γ-radiation-induced injury in mice.
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Protective effect of Sesamol against 60Co γ-ray-induced hematopoietic and gastrointestinal injury in C57BL/6 male mice
2015Co-Authors: Shahanshah Khan, J S Adhikari, Moshahid Alam M Rizvi, A. Kumar, N K ChaudhuryAbstract:Protection of γ-ray-induced injury in hematopoietic and gastrointestinal (GI) systems is the rationale behind developing radioprotectors. The objective of this study, therefore, was to investigate the radioprotective efficacy and mechanisms underlying Sesamol in amelioration of γ-ray-induced hematopoietic and GI injury in mice. C57BL/6 male mice were pre-treated with a single dose (100 or 50 mg/kg, 30 min prior) of Sesamol through the intraperitoneal route and exposed to LD50/30 (7.5 Gy) and sublethal (5 Gy) dose of γ-radiation. Thirty-day survival against 7.5 Gy was monitored. Sesamol (100 mg/kg) pre-treatment reduced radiation-induced mortality and resulted survival of about 100% against 7.5 Gy of γ-irradiation. Whole-body irradiation drastically depleted hematopoietic progenitor stem cells in bone marrow, B cells, T cell subpopulations, and splenocyte proliferation in the spleen on day 4, which were significantly protected in Sesamol pre-treated mice. This was associated with a decrease of radiation-induced micronuclei (MN) and apoptosis in bone marrow and spleen, respectively. Sesamol pre-treatment inhibited lipid peroxidation, translocation of gut bacteria to spleen, liver, and kidney, and enhanced regeneration of crypt cells in the GI system. In addition, Sesamol pre-treatment reduced the radiation-induced pattern of expression of p53 and Bax apoptotic proteins in the bone marrow, spleen, and GI. This reduction in apoptotic proteins was associated with the increased anti-apoptotic-Bcl-x and PCNA proteins. Further, assessment of antioxidant capacity using ABTS and DPPH assays revealed that Sesamol treatment alleviated total antioxidant capacity in spleen and GI tissue. In conclusion, the results of the present study suggested that Sesamol as a single prophylactic dose protects hematopoietic and GI systems against γ-radiation-induced injury in mice.
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Sesamol as a potential radioprotective agent in vitro studies
Radiation Research, 2011Co-Authors: Krishnanand Mishra, P S Srivastava, N K ChaudhuryAbstract:Protection against radiation-induced DNA strand breaks is an important aspect in the design and development of a radioprotector. In this study, the radioprotective efficacy of Sesamol, a natural antioxidant, was investigated in aqueous solution of plasmid DNA (pBR322) and compared with that of melatonin, a known antioxidant-based radioprotector. Thermal denaturation studies on irradiated calf thymus DNA were also carried out with Sesamol and melatonin. Sesamol demonstrated greater radioprotective efficacy in both plasmid DNA and calf thymus DNA. To assess the radical scavenging capacity of Sesamol and melatonin, 2deoxyribose degradation, DPPH and ABTS assays were performed. Sesamol exhibited more scavenging capacity compared to melatonin. In vitro studies with V79 cells showed that Sesamol is 20 times more potent than melatonin. It is proposed that the greater radioprotective efficacy of Sesamol could be due to its greater capacity for scavenging of free radicals compared to melatonin. The results will be helpful in understanding the mechanisms and development of Sesamol
Paola Dangelo - One of the best experts on this subject based on the ideXlab platform.
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fate of a deep eutectic solvent upon cosolvent addition choline chloride Sesamol 1 3 mixtures with methanol
ACS Sustainable Chemistry & Engineering, 2021Co-Authors: Matteo Busato, Valentina Migliorati, Alessandra Del Giudice, Valerio Di Lisio, Pierpaolo Tomai, Alessandra Gentili, Andrea Martinelli, Paola DangeloAbstract:The changes upon methanol (MeOH) addition in the structural arrangement of the highly eco-friendly deep eutectic solvent (DES) formed by choline chloride (ChCl) and Sesamol in 1:3 molar ratio have been studied by means of attenuated total reflection Fourier transform infrared spectroscopy, small- and wide-angle X-ray scattering (SWAXS), and molecular dynamics simulations. The introduction of MeOH into the DES promotes the increase of the number of Cl-MeOH hydrogen bonds (HBs) through the replacement of Sesamol and choline molecules from the chloride anion coordination sphere. This effect does not promote the Sesamol-Sesamol, choline-choline, and Sesamol-choline interactions, which remain as negligible as in the pure DES. Differently, the displaced Sesamol and choline molecules are solvated by MeOH, which also forms HBs with other MeOH molecules, so that the system arranges itself to keep the overall amount of HBs maximized. SWAXS measurements show that this mechanism is predominant up to MeOH/DES molar ratios of 20-24, while after this ratio value, the scattering profile is progressively diluted in the cosolvent background and decreases toward the signal of pure MeOH. The ability of MeOH to interplay with all of the DES components produces mixtures with neither segregation of the components at nanoscale lengths nor macroscopic phase separation even for high MeOH contents. These findings have important implications for application purposes since the understanding of the pseudophase aggregates formed by a DES with a dispersing cosolvent can help in addressing an efficient extraction procedure.
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anatomy of a deep eutectic solvent structural properties of choline chloride Sesamol 1 3 compared to reline
Physical Chemistry Chemical Physics, 2021Co-Authors: Matteo Busato, Valentina Migliorati, Alessandra Del Giudice, Valerio Di Lisio, Pierpaolo Tomai, Alessandra Gentili, Paola DangeloAbstract:The structural properties of the deep eutectic solvent (DES) formed by choline chloride (ChCl) and Sesamol in 1 : 3 ratio have been investigated and compared to those of reline (ChCl : urea 1 : 2). An integrated approach combining small and wide angle X-ray scattering with molecular dynamics simulations has been employed and the simulation protocol has been validated against the experimental data. In the ChCl : Sesamol DES, strong hydrogen bonds (HBs) are formed between the chloride anion and the hydroxyl groups of the choline and of Sesamol molecules. Conversely, choline–choline, choline–Sesamol and Sesamol–Sesamol interactions are negligible. A more extended interplay between the constituents is observed in reline where, besides the HBs involving the chloride anion, the eutectic formation is favored also by strong choline–urea and urea–urea interactions. The three-dimensional arrangement around the individual components shows that, in the ChCl : Sesamol DES, the cholinium cations and the Sesamol molecules are packed in such a way to maximize the interactions with the chlorine anion. This structural arrangement may favor the π–π interactions between the Sesamol molecules and the aromatic species mediated by the chloride ions, providing an interpretation for the high separation rates previously observed for phenolic DESs towards aromatic compounds.
Sridevi Annapurna Singh - One of the best experts on this subject based on the ideXlab platform.
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inhibition of lipoxygenase by Sesamol corroborates its potential anti inflammatory activity
International Journal of Biological Macromolecules, 2017Co-Authors: P S Yashaswini, A Appu G Rao, Sridevi Annapurna SinghAbstract:Reactive oxygen species, the byproducts of oxygenases reaction, when in excess, promote degenerative diseases like cardiovascular, cancer and arthritis. Sesame lignans- sesamin, Sesamolin and the phenolic degradation product of Sesamolin, Sesamol, are empirically known for their health promoting properties like antioxidant, antimutagenic, antiaging and antiinflammatory activities. In the current study, the effect of Sesamol on the inflammatory oxygenase - lipoxygenase (LOX) was investigated. Enzyme kinetics and spectroscopic techniques were used to understand the inhibition mechanism. Sesamol was a potent inhibitor of soy LOX-1. It inhibited soy LOX-1 in a dose dependent manner with IC50 value of 51.84μM and Ki of 4.9μM. Binding studies using circular dichroism and corroborated by surface plasmon resonance, revealed that Sesamol does not bind or change the conformation of LOX. Further, Sesamol prevented the conversion of inactive LOX (Fe2+) to active LOX (Fe3+) by arresting the oxidation state of iron and prolonging the lag phase by virtue of its ability to scavenge hydroperoxides. Understanding the mechanism of action of such molecules will help in their application and promotion as nutraceuticals.
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bioactive lignans from sesame sesamum indicum l evaluation of their antioxidant and antibacterial effects for food applications
Journal of Food Science and Technology-mysore, 2015Co-Authors: Mahendra C Kumar, Sridevi Annapurna SinghAbstract:Antioxidants protect the body from various disease conditions through their ability to neutralize the effects of free radicals. Synthetic antioxidants are extensively used in processed foods for prevention of oxidation and retention of sensory quality. Consumer awareness and preference has led to a vigorous interest in the search for natural antioxidants. Sesamin and Sesamolin, the major lignans present in sesame oil, are known for their antioxidative properties. Roasted sesame oil has a higher concentration of Sesamol, the thermally degraded product of Sesamolin, which is considered a more potent antioxidant compared to its parent molecule. The isolated lignans and Sesamol were tested for their antioxidant, free radical scavenging and antibacterial properties. Sesamol is the best antioxidant and free radical scavenger amongst the molecules studied with IC50 value of 5.44 μg / mL (DPPH radical scavenging activity). Antibacterial assays against food borne pathogens revealed Sesamol to be an antimicrobial agent with minimal inhibitory concentration (MIC) of 2 mg /mL in the culture. Its activity was synergistic with γ-tocopherol, also present in sesame seeds. Inhibition of browning (60–65 %) in fruit pulps (apple, banana and potato) was observed in presence of 20 μM Sesamol.
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interaction of Sesamol 3 4 methylenedioxyphenol with tyrosinase and its effect on melanin synthesis
Biochimie, 2011Co-Authors: Mahendra C Kumar, U V Sathisha, Shylaja M Dharmesh, A Appu G Rao, Sridevi Annapurna SinghAbstract:Sesamin, Sesamolin (lignans) and Sesamol--from sesame seed (Sesamum indicum L.)--are known for their health promoting properties. We examined the inhibition effect of Sesamol, a phenolic degradation product of Sesamolin, on the key enzyme in melanin synthesis, viz. tyrosinase, in vitro. Sesamol inhibits both diphenolase and monophenolase activities with midpoint concentrations of 1.9 μM and 3.2 μM, respectively. It is a competitive inhibitor of diphenolase activity with a K(i) of 0.57 μM and a non-competitive inhibitor of monophenolase activity with a K(i) of 1.4 μM. Sesamol inhibits melanin synthesis in mouse melanoma B16F10 cells in a concentration dependant manner with 63% decrease in cells exposed to 100 μg/mL Sesamol. Apoptosis is induced by Sesamol, limiting proliferation. This study of the chemistry and biology of lignans, in relation to the mode of action of bioactive components, may open the door for drug applications targeting enzymes.
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effect of infrared heating on the formation of Sesamol and quality of defatted flours from sesamum indicum l
Journal of Food Science, 2009Co-Authors: Mahendra C Kumar, A Appu G Rao, Sridevi Annapurna SinghAbstract:Infrared (IR) heating offers several advantages over conventional heating in terms of heat transfer efficiency, compactness of equipment, and quality of the products. Roasting of sesame seeds degrades the lignan Sesamolin to Sesamol, which increases the oxidative stability of sesame oil synergistically with tocopherols. IR (near infrared, 1.1 to 1.3 microm, 6 kW power) roasting conditions were optimized for the conversion of Sesamolin to Sesamol. The resultant oil was evaluated for Sesamol and tocopherol content as well as oxidative stability. The defatted flours were evaluated for their nutritional content and functionality. IR roasting of sesame seeds at 200 degrees C for 30 min increased the efficiency of conversion of Sesamolin to Sesamol (51% to 82%) compared to conventional heating. The gamma-tocopherol content decreased by 17% and 25% in oils treated at 200 and 220 degrees C for 30 min, respectively. There were no significant differences in the tocopherol content and oxidative stability of the oil. Methionine and cysteine content of the flours remained unchanged due to roasting. The functional properties of defatted flours obtained from either IR roasted or conventionally roasted sesame seeds remained the same. Practical Applications: Sesame oil is stable to oxidation compared to other vegetable oils. This stability can be attributed to the presence of tocopherols and the formation of Sesamol, the thermal degradation product of Sesamolin-a lignan present in sesame. Roasting of sesame seeds before oil extraction increases Sesamol content which is a more potent antioxidant than the parent molecule. The conversion efficiency of Sesamolin to Sesamol is increased by 31% by infrared roasting of seeds compared to electric drum roasting. This can be used industrially to obtain roasted oil with greater oxidative stability.