The Experts below are selected from a list of 1086 Experts worldwide ranked by ideXlab platform
Ali Honaramooz - One of the best experts on this subject based on the ideXlab platform.
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Research Article The Effects of Elk Velvet Antler Dietary Supplementation on Physical Growth and Bone Development in Growing Rats
2016Co-Authors: Jiongra Che, Yanfei Yang, Sepideh Abbasi, Daryoush Hajinezhad, Saija A Kontulaine, Ali HonaramoozAbstract:which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Elk Velvet Antler (EVA) has been used in traditional Oriental medicine for centuries to promote general health; however, little evidence for its effect on bone development is available. We investigated the effects of lifelong exposure of Wistar rats to a diet containing 10%EVAon physical growth and bone development.Measurements includedweekly bodyweights, blood chemistry and kidney and testis/ovary indices (sacrificed at 5, 9, or 16 weeks of age), and bone traits of the femur bones by peripheral quantitative computed tomography (pQCT). Mean body weights were higher in the EVA group at 4–8 weeks in males and at 5 weeks of age in females. The kidney indices were greater in EVA dietary supplemented male rats at 5 and 16 weeks of age, in females at 16 weeks of age, and testis/ovary indices at 5 weeks of age. The femoral length was increased in both males and females at 5 weeks, and several pQCT-measured parameters had increased in EVAmales and females.The activity of alkaline phosphatase (ALP) increased in EVA group while the content of calcium and phosphorus did not differ among groups. Our results seem to support a role for dietary supplementation of EVA on growth and bone development in this model. 1
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the effects of elk Velvet Antler dietary supplementation on physical growth and bone development in growing rats
Evidence-based Complementary and Alternative Medicine, 2015Co-Authors: Jiongra Che, Yanfei Yang, Sepideh Abbasi, Daryoush Hajinezhad, Saija A Kontulaine, Ali HonaramoozAbstract:Elk Velvet Antler (EVA) has been used in traditional Oriental medicine for centuries to promote general health; however, little evidence for its effect on bone development is available. We investigated the effects of lifelong exposure of Wistar rats to a diet containing 10% EVA on physical growth and bone development. Measurements included weekly body weights, blood chemistry and kidney and testis/ovary indices (sacrificed at 5, 9, or 16 weeks of age), and bone traits of the femur bones by peripheral quantitative computed tomography (pQCT). Mean body weights were higher in the EVA group at 4–8 weeks in males and at 5 weeks of age in females. The kidney indices were greater in EVA dietary supplemented male rats at 5 and 16 weeks of age, in females at 16 weeks of age, and testis/ovary indices at 5 weeks of age. The femoral length was increased in both males and females at 5 weeks, and several pQCT-measured parameters had increased in EVA males and females. The activity of alkaline phosphatase (ALP) increased in EVA group while the content of calcium and phosphorus did not differ among groups. Our results seem to support a role for dietary supplementation of EVA on growth and bone development in this model.
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Research Article Dietary Supplementation of Female Rats with Elk Velvet Antler Improves Physical and Neurological Development of Offspring
2013Co-Authors: Jiongra Che, Murray R Woodbury, Jane Alco, Ali HonaramoozAbstract:which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Elk Velvet Antler (EVA) has a traditional use for promotion of general health. However, evidence of EVA effects at different lifestages is generally lacking. This paper investigated the effects of long-term maternal dietary EVA supplementation on physical, reflexological and neurological development of rat offspring. Female Wistar rats were fed standard chow or chow containing 10% EVA for 90 days prior to mating and throughout pregnancy and lactation. In each dietary group, 56 male and 56 female pups were assessed for physical, neuromotor, and reflexologic development postnatally. Among the examined physical developmental parameters, incisor eruption occurred one day earlier in pups nursing dams receiving EVA. Among neuromotor developmental parameters, duration of supported and unsupported standing was longer for pups nursing EVA supplemented dams. Acquisition of neurological reflex parameters (righting reflex, negative geotaxis, cliff avoidance acoustic startle) occurred earlier in pup
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dietary supplementation of female rats with elk Velvet Antler improves physical and neurological development of offspring
Evidence-based Complementary and Alternative Medicine, 2012Co-Authors: Jiongra Che, Murray R Woodbury, Jane Alco, Ali HonaramoozAbstract:Elk Velvet Antler (EVA) has a traditional use for promotion of general health. However, evidence of EVA effects at different lifestages is generally lacking. This paper investigated the effects of long-term maternal dietary EVA supplementation on physical, reflexological and neurological development of rat offspring. Female Wistar rats were fed standard chow or chow containing 10% EVA for 90 days prior to mating and throughout pregnancy and lactation. In each dietary group, 56 male and 56 female pups were assessed for physical, neuromotor, and reflexologic development postnatally. Among the examined physical developmental parameters, incisor eruption occurred one day earlier in pups nursing dams receiving EVA. Among neuromotor developmental parameters, duration of supported and unsupported standing was longer for pups nursing EVA supplemented dams. Acquisition of neurological reflex parameters (righting reflex, negative geotaxis, cliff avoidance acoustic startle) occurred earlier in pups nursing dams receiving EVA. Longterm maternal EVA supplementation prior to and during pregnancy and lactation accelerated certain physical, reflexologic, and neuromotor developmental milestones and caused no discernible adverse effects on developing offspring. The potential benefits of maternal EVA supplementation on postnatal development warrants further investigation to determine whether EVA can be endorsed for the promotion of maternal and child health.
Feng Wei - One of the best experts on this subject based on the ideXlab platform.
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identification of Velvet Antler and its mixed varieties by uplc qtof ms combined with principal component analysis
Journal of Pharmaceutical and Biomedical Analysis, 2019Co-Authors: Xiaoha Guo, Xianlong Cheng, Wenxi Liu, Feng WeiAbstract:Abstract Many species of Velvet Antler have been used as traditional medicine for thousands of years; however, as medicinal materials, Velvet Antler derived from different animals have different clinical effects. To distinguish the differences and homologies, ultra-performance liquid chromatography-quadruple-time of flight mass spectrometry (UPLC-QTOF-MS) coupled with principal component analysis (PCA) was developed and applied to identify these Antler samples derived from Cervus nippon Temminck, Cervus elaphus Linnaeus and Rangifer tarandus Linnaeus, which were first tested and compared at the molecular level of protein. The UPLC-MS data of the trypsin digested samples were subjected to PCA, and the potential markers based on peptide were depicted to illustrate their differences. With the integrated strategy combining UPLC-QTOF-MS with PCA, the results from this study indicated that the proposed methods could be successfully applied to distinguish reindeer Antler from sika deer Antler and red deer Antler, which were prescribed in the Chinese Pharmacopoeia (2015 edition).
Mingju Che - One of the best experts on this subject based on the ideXlab platform.
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Comparison of anti-inflammatory effect and protein profile between the water extracts from Formosan sambar deer and red deer
Elsevier, 2018Co-Authors: Chingyu Kuo, Yi-ting Cheng, Mingju CheAbstract:Velvet Antler (VA), the unossified Antler from members of the family Cervidae, has been used in traditional Chinese medicines and health foods for over 2000 years in enhancement of kidney function and treatment or prevention of cardiovascular, immunological and gynaecological disease. The aim of this study was to investigate the anti-inflammatory effect of Velvet Antler water extracts from Formosan sambar deer (Rusa unicolor swinhoei, SVAE) and red deer (Cervus elaphus, RVAE). Results indicated that both SVAE and RVAE significantly reduced the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) productions in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells at concentrations above 200 μg mL−1. SVAE seems to demonstrate a better anti-inflammatory effect than that of RVAE in vitro. Both SVAE and RAVE also enhanced the anti-inflammatory cytokine IL-10 production in LPS-stimulated RAW 264.7 cells. The results of MTT assay indicated that SVAE and RVAE did not exhibit any cytotoxicity in LPS-stimulated RAW 264.7 cells. Two-dimensional (2D) gel electrophoresis analysis revealed that the levels of 6 specific proteins were different between these two Velvet Antlers samples. Furthermore, the storage period was the major factor affecting the anti-inflammatory activity of SAVE. In this study, we demonstrated the difference of anti-inflammatory effect and the protein profile between SVAE and RVAE. SVAE showed better anti-inflammatory potential than RVAE. In the future, the anti-inflammatory active components and their related mechanisms should be further investigated. Keywords: Velvet Antler, Traditional Chinese medicine, Formosan sambar deer, Anti-inflammatory effect, 2D SDS-PAGE electrophoresi
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effect of the Velvet Antler of formosan sambar deer cervus unicolor swinhoei on the prevention of an allergic airway response in mice
Evidence-based Complementary and Alternative Medicine, 2012Co-Authors: Chingyu Kuo, Tingyeu Dai, Chihhua Wang, Kunna Che, Yenpo Che, Ting Wang, Mingju CheAbstract:Two mouse models were used to assay the antiallergic effects of the Velvet Antler (VA) of Formosan sambar deer (Cervus unicolor swinhoei) in this study. The results using the ovalbumin- (OVA-) sensitized mouse model showed that the levels of total IgE and OVA-specific IgE were reduced after VA powder was administrated for 4 weeks. In addition, the ex vivo results indicated that the secretion of T helper cell 1 (Th1), regulatory T (Treg), and Th17 cytokines by splenocytes was significantly increased () when VA powder was administered to the mice. Furthermore, OVA-allergic asthma mice that have been orally administrated with VA powder showed a strong inhibition of Th2 cytokine and proinflammatory cytokine production in bronchoalveolar fluid compared to control mice. An increase in the regulatory T-cell population of splenocytes in the allergic asthma mice after oral administration of VA was also observed. All the features of the asthmatic phenotype, including airway inflammation and the development of airway hyperresponsiveness, were reduced by treatment with VA. These findings support the hypothesis that oral feeding of VA may be an effective way of alleviating asthmatic symptoms in humans.
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the antiinfective effects of Velvet Antler of formosan sambar deer cervus unicolor swinhoei on staphylococcus aureus infected mice
Evidence-based Complementary and Alternative Medicine, 2011Co-Authors: Tingyeu Dai, Chihhua Wang, Kunna Che, Inung Huang, Weisheng Hong, Shengyao Wang, Yenpo Che, Chingyu Kuo, Mingju CheAbstract:We assayed the effects of Velvet Antler (VA) of Formosan sambar deer (Cervus unicolor swinhoei) and its extracts on the anti-infective activity against pathogenic Staphylococcus aureus in vitro and in vivo in this study. In vitro data indicated that the VA extracts stimulated the proliferation of resting splenocytes and macrophages in a dose-dependent manner up to the highest concentration used (150 μg mL−1). The production of proinflammatory cytokines (TNF-α, IL-6, IL-12) by lipoteichoic acid was significantly suppressed after being cocultured with the VA extracts in a dose-dependent manner. Animal test in S. aureus-infected mice demonstrated that the numbers of bacteria determined in the kidneys and peritoneal lavage fluid of S. aureus-infected mice were significantly higher than those found in the same organs of mice pretreated with the VA samples. Moreover, the highly enhanced phagocytic activity of macrophages was further verified after in vitro treatment with the VA samples. The protective mechanisms of the VA samples might include an immune enhancer and an inflammatory cytokine suppressor.
Xiqing Yue - One of the best experts on this subject based on the ideXlab platform.
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a recombinant polypeptide from Velvet Antler of cervus nippon temminck exhibits similar immunomodulatory effects as its natural counterpart
Immunopharmacology and Immunotoxicology, 2016Co-Authors: Enhui Zha, Li Danda, Tiezhong Zhou, Gao Shenyang, Xiqing YueAbstract:AbstractContext: Velvet Antler (VA) is recognized as one of the most important Chinese traditional medical herbs. To date, the immunoactivity of the single component of VA is rarely studied though its compound extracts have been well analyzed.Objective: The current study was designed to evaluate the immunomodulatory effects of a recombinant polypeptide (rVAP32) based on the VA of the sika deer by comparison with its natural counterpart (nVAP32).Materials and methods: Splenocytes proliferation and NK-cell cytotoxicity assay was evaluated by the WST-8 colorimetric method. CD4+/CD8+ cell subpopulations regulation was screened by the flowcytometry method and the Th1 or Th2-related cytokine production was measured by ELISA.Results: In vitro tests showed that both rVAP32 and nVAP32 could significantly stimulate splenocytes proliferation and enhance the NK-cell cytotoxicity and CD4+/CD8+ cell subpopulations when compared with the irrelevant peptide and blank control groups. Also, they demonstrated a significant ...
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immunomodulatory effects of a 3 2kda polypeptide from Velvet Antler of cervus nippon temminck
International Immunopharmacology, 2013Co-Authors: Enhui Zha, Xuesong Guo, Shenyang Gao, Xiqing YueAbstract:The objective of this study was to evaluate the immunomodulatory effects of a native 3.2kDa polypeptide of Velvet Antler from sika deer (nVAP32) on BALB/c mice immunocytes. In vitro tests showed that nVAP32 significantly stimulated splenocyte proliferation and enhanced the NK cytotoxicity and CD4(+)/CD8(+) cell subpopulations. Also, nVAP32 demonstrated a significant capacity in up- and down-regulating the expression of Th1- and Th2-related cytokines respectively. These results indicated that nVAP32 might have potential immunomodulatory effects on the immune system of mice and the further investigation on in vivo effects is qualified.
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wound healing by a 3 2 kda recombinant polypeptide from Velvet Antler of cervus nippon temminck
Biotechnology Letters, 2012Co-Authors: Enhui Zha, Shenyang Gao, Yutai Wang, Xiqing YueAbstract:Velvet Antler (VA) is used in traditional Chinese medicine to treat a wide range of ailments including the enhancement of wound healing. A 3.2 kDa recombinant polypeptide of VA from sika deer was purified and compared to native polypeptides stimulation growth of NIH3T3 cells. Both stimulated growth in a dose-dependent manner (10-100 μg/ml). To study its wound healing properties, burn-wounded rats were topically administered with recombinant VA polypeptide or native polypeptide. Rats treated with 0.05 and 0.1% (w/w) polypeptides exhibited significant wound healing. As the yield of recombinant polypeptide was 40-fold higher than that of the native polypeptide, it may therefore be a useful biopharmaceutical.
Seunghong Lee - One of the best experts on this subject based on the ideXlab platform.
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protective effects of novel antioxidant peptide purified from alcalase hydrolysate of Velvet Antler against oxidative stress in chang liver cells in vitro and in a zebrafish model in vivo
International Journal of Molecular Sciences, 2019Co-Authors: Yuling Ding, Sangho Moo, Seunghong LeeAbstract:Velvet Antler has a long history in traditional medicine. It is also an important healthy ingredient in food as it is rich in protein. However, there has been no report about antioxidant peptides extracted from Velvet Antler by enzymatic hydrolysis. Thus, the objective of this study was to hydrolyze Velvet Antler using different commercial proteases (Acalase, Neutrase, trypsin, pepsin, and α-chymotrypsin). Antioxidant activities of different hydrolysates were investigated using peroxyl radical scavenging assay by electron spin resonance spectrometry. Among all enzymatic hydrolysates, Alcalase hydrolysate exhibited the highest peroxyl radical scavenging activity. Alcalase hydrolysate was then purified using ultrafiltration, gel filtration, and reverse-phase high performance liquid chromatography. The purified peptide was identified to be Trp-Asp-Val-Lys (tetrapeptide) with molecular weight of 547.29 Da by Q-TOF ESI mass spectroscopy. This purified peptide exhibited strong scavenging activity against peroxyl radical (IC50 value, 0.028 mg/mL). In addition, this tetrapeptide showed significant protection ability against AAPH-induced oxidative stress by inhibiting of reactive oxygen species (ROS) generation in Chang liver cells in vitro and in a zebrafish model in vivo. This research suggests that the tetrapeptide derived from Alcalase-proteolytic hydrolysate of Velvet Antler are excellent antioxidants and could be effectively applied as functional food ingredients and pharmaceuticals.
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enzymatic hydrolysate from Velvet Antler suppresses adipogenesis in 3t3 l1 cells and attenuates obesity in high fat diet fed mice
Excli Journal, 2017Co-Authors: Yuling Ding, Yong Tae Jeo, Sangho Moo, Yanmei Wang, Seunghong LeeAbstract:The purpose of the current study was to investigate the potential anti-obesity activity of an enzymatic hydrolysate of Velvet Antler in inhibiting adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-fed obese mice. The enzymatic hydrolysate was prepared using the commercial food grade protease, Protamex. The Velvet Antler Protamex hydrolysate (VAPH) indicated profound inhibitory effects on adipogenesis dose-dependently by decreasing the accumulation of triglycerides and down-regulating expression levels of adipogenesis-related proteins C/EBPα, SREBP-1, and PPARγ. In a mouse model of HFD-induced obesity, oral administration of VAPH (100 and 300 mg/kg for 13 weeks) significantly reduced the body weight gain that had resulted from the HFD. VAPH treatment also lowered the serum glucose and triglyceride levels, while increasing the HDL-C level. Furthermore, the treatment greatly reduced hepatic lipid droplet accumulation as well as the size of adipocytes. Current findings demonstrate that VAPH has profound anti-obesity effects and could be an effective candidate for preventing obesity and obesity-related chronic diseases.
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anti inflammatory effects of enzymatic hydrolysates of Velvet Antler in raw 264 7 cells in vitro and zebrafish model
Excli Journal, 2015Co-Authors: Seunghong Lee, Yong Tae Jeo, Sangho Moo, Youji Jeo, Yanmei Wang, Yuling Ding, Hyewo Yang, Siheung SungAbstract:Enzymatic hydrolysis has been successfully used for the extraction of numerous biologically active components from a wide variety of natural sources. In the present study, Velvet Antler was subjected to the extraction process using Alcalase protease. We analyzed bioactive components, such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, present in the Velvet Antler Alcalase hydrolysate (VAAH) and assessed their anti-inflammatory effects in zebrafish as well as in vitro using cell lines. VAAH mainly contained uronic acid (78.22 mg/g) and sulfated-GAGs (50.47 mg/g), while the amount of sialic acid was negligible (5.55 mg/g). VAAH inhibited the production of nitric oxide (NO) by lipopolysaccharide (LPS)-induced cells in a dose-dependent manner and the inhibitory effect of VAAH on NO production was higher than that of hot water extracts. VAAH treatment also reduced the expression of inflammatory mediators such as nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, we evaluated anti-inflammatory effects of VAAH using LPS-stimulated zebrafish. Treatment with LPS significantly increased cell death, NO, and reactive oxygen species (ROS) levels in zebrafish. Notably, VAAH significantly inhibited the extent of LPS-stimulated cell death and generation of NO and ROS in zebrafish. These results suggest that VAAH alleviated inflammation and cell death by inhibiting the generation of ROS induced by LPS treatment. Thus, VAAH could be used as a potential natural remedy with a strong anti-inflammatory effect. Taken together, we believe that based on our present results, enzymatic hydrolysis of Velvet Antler may be an effective process to make Antler products acceptable as elements of health foods and nutraceutical components with increased biological activity.
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effects of Antler development stage on fatty acid vitamin and gags contents of Velvet Antler in spotted deer cervus nippon
Asian-australasian Journal of Animal Sciences, 2007Co-Authors: Seunghong Lee, Yong Tae Jeo, Myeong Hwa Kim, S M Lee, Seongji Kim, Sangho MooAbstract:This study aimed to provide basic information as the foundation for further studies on the assessment of Velvet Antler quality by investigating the changes in fatty acid, vitamin A and E, minerals and GAGs contents by development stage of Antler in spotted deer (Cervus nippon). Twelve stags (aged 4 to 5 years) were divided into two groups and Velvet Antler harvested 40 days (FDG) and 60 days (SDG) after casting of the buttons from the previous set. Total saturated fatty acid was lower in FDG than SDG (p<0.05). Total monounsaturated fatty acid, conjugated linoleic acid (p<0.05), polyunsaturated fatty acid and ω3 fatty acid were higher in FDG than SDG. The vitamin A content of FDG was higher than that of SDG, but the vitamin E content of FDG was lower than that of SDG (p<0.05). SDG had significantly higher calcium and phosphate content than FDG (p<0.05). The magnesium content showed a similar trend to the contents of calcium and phosphate, but there was no significant difference between SDG and FDG. Uronic acid content was higher in FDG than SDG but there was no significant difference. The contents of GAGs and sialic acid were significantly higher (p<0.05) in FDG than SDG. These results indicated that the longer stage of Antler development had lower content of activating components and this lead to a decrease of Antler quality.
