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1-Bromopropane

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Gaku Ichihara – One of the best experts on this subject based on the ideXlab platform.

  • Role of cytochrome P450s in the male reproductive toxicity of 1-Bromopropane.
    Toxicology research, 2016
    Co-Authors: Cai Zong, Toshihiro Sakurai, C. Edwin Garner, Chinyen Huang, Xiao Zhang, Jie Chang, Masashi Kato, Sahoko Ichihara, Gaku Ichihara

    Abstract:

    1-Bromopropane (1BP) is widely used as an alternative to ozone-depleting solvents. The present study investigated the role of P450s in 1BP-induced male reproductive toxicity. Mice co-treated with 1-aminobenzotriazole (ABT), a non-selective P450 inhibitor, were exposed to 1BP at 0, 50, 250, or 1200 ppm, while saline-treated control mice were exposed to 1BP at 0, 50, or 250 ppm, for 4 weeks. In the saline-treated mice, exposure to 1BP at 250 ppm decreased the sperm count and sperm motility. Histopathological examination showed that exposure to 1BP at 50 and 250 ppm increased the number of elongated spermatids retained at the basal region of stage IX, X and XI seminiferous tubules, while exposure to 1BP at 250 ppm increased the number of periodic acid-Schiff (PAS)-positive round structures in stage IX, X, and XI seminiferous tubules. Co-treatment with ABT prevented the above changes induced by exposure to 1BP at 50 or 250 ppm. However, ABT-treated mice exposed to 1BP in the 1200 ppm group showed decreases in the weights of reproductive organs, epididymal sperm count and motility, increases in epididymal sperm with abnormal heads, retained spermatids and PAS-positive round structures in stages IX–XI, depletion of spermatogenic cells in part of the seminiferous tubules, and a small number of round spermatids in stage VII seminiferous tubules. The results at 50 and 250 ppm of 1-BP exposure indicate that P450s play important roles in 1BP-induced testicular toxicity. The control of P450 activity reduced 1BP-induced male reproductive toxicities including spermiation failure, reduction of epididymal sperm count and motility, and formation of PAS-positive round structures at postspermiation stages.

  • Hippocampal phosphoproteomics of F344 rats exposed to 1-Bromopropane.
    Toxicology and applied pharmacology, 2014
    Co-Authors: Zhenlie Huang, Lingyi Zhang, Jie Chang, Sahoko Ichihara, Shinji Oikawa, Hanlin Huang, Gaku Ichihara

    Abstract:

    1-Bromopropane (1-BP) is neurotoxic in both experimental animals and human. To identify phosphorylated modification on the unrecognized post-translational modifications of proteins and investigate their role in 1-BP-induced neurotoxicity, changes in hippocampal phosphoprotein expression levels were analyzed quantitatively in male F344 rats exposed to 1-BP inhalation at 0, 400, or 1000 ppm for 8 h/day for 1 or 4 weeks. Hippocampal protein extracts were analyzed qualitatively and quantitatively by Pro-Q Diamond gel staining and SYPRO Ruby staining coupled with two-dimensional difference in gel electrophoresis (2D-DIGE), respectively, as well as by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to identify phosphoproteins. Changes in selected proteins were further confirmed by Manganese II (Mn2 +)-Phos-tag SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Bax and cytochrome c protein levels were determined by western blotting. Pro-Q Diamond gel staining combined with 2D-DIGE identified 26 phosphoprotein spots (p < 0.05), and MALDI-TOF/MS identified 18 up-regulated proteins and 8 down-regulated proteins. These proteins are involved in the biological process of response to stimuli, metabolic processes, and apoptosis signaling. Changes in the expression of phosphorylated 14-3-3 θ were further confirmed by Mn2 +-Phos-tag SDS-PAGE. Western blotting showed overexpression of Bax protein in the mitochondria with down-regulation in the cytoplasm, whereas cytochrome c expression was high in the cytoplasm but low in the mitochondria after 1-BP exposure. Our results suggest that the pathogenesis of 1-BP-induced hippocampal damage involves inhibition of antiapoptosis process. Phosphoproteins identified in this study can potentially serve as biomarkers for 1-BP-induced neurotoxicity.

  • Effects of sub-acute and sub-chronic inhalation of 1-Bromopropane on neurogenesis in adult rats.
    Toxicology, 2012
    Co-Authors: Lingyi Zhang, Sahoko Ichihara, Taku Nagai, Kiyofumi Yamada, Daisuke Ibi, Kaviarasan Subramanian, Zhenlie Huang, Sahabudeen Sheik Mohideen, Hisao Naito, Gaku Ichihara

    Abstract:

    a b s t r a c t Purpose: 1-Bromopropane (1-BP) intoxication is associated with depression and cognitive and memory deficits. The present study tested the hypothesis that 1-BP suppresses neurogenesis in the dentate gyrus, which is involved in higher cerebral function, in adult rats.

Tae Cheon Jeong – One of the best experts on this subject based on the ideXlab platform.

  • Identification of a N 7 -guanine adduct of 1-Bromopropane in calf thymus DNA by mass spectrometry
    Molecular & Cellular Toxicology, 2016
    Co-Authors: Pritam Thapa, Mahesh Raj Nepal, Ki Sun Jeong, Mi Jeong Kang, Hye Gwang Jeong, Eun-kyung Kim, Keumhan Noh, Sangkyu Lee, Jun-ho Lee, Tae Cheon Jeong

    Abstract:

    As a replacement for chlorofluorocarbons that cause ozone depletion, 1-Bromopropane has been widely used in work place. In the present study, the formation of N 7-guanine adduct in DNA by 1-Bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N 7-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2ʹ-deoxyguanosine with 1-Bromopropane produced N 7-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-Bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-Bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-Bromopropane would be associated with the adduct formation on DNA at least in part.

  • Identification of a N
    ^7-guanine adduct of 1-Bromopropane in calf thymus DNA by mass spectrometry
    Molecular & Cellular Toxicology, 2016
    Co-Authors: Pritam Thapa, Mahesh Raj Nepal, Ki Sun Jeong, Mi Jeong Kang, Hye Gwang Jeong, Tae Cheon Jeong

    Abstract:

    As a replacement for chlorofluorocarbons that cause ozone depletion, 1-Bromopropane has been widely used in work place. In the present study, the formation of N ^7-guanine adduct in DNA by 1-Bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N ^7-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2ʹ-deoxyguanosine with 1-Bromopropane produced N ^7-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-Bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-Bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-Bromopropane would be associated with the adduct formation on DNA at least in part.

  • Role of metabolism in 1-Bromopropane-induced hepatotoxicity in mice.
    Journal of toxicology and environmental health. Part A, 2010
    Co-Authors: Sangkyu Lee, Mi Jeong Kang, Hye Gwang Jeong, Tae Won Jeon, Jin Woo Yoo, Wonku Kang, Won Seok Lyoo, Tae Cheon Jeong

    Abstract:

    A possible role of metabolism in 1-Bromopropane (1-BP)-induced hepatotoxicity was investigated in male ICR mice. The depletion of glutathione (GSH) by formation of GSH conjugates was associated with increased hepatotoxicity in 1-BP-treated mice. The formation of S-propyl and 2-hydroxypropyl GSH conjugates were identified in the liver following 1-BP treatment. In addition, the formation of reactive metabolites of 1-BP by certain cytochrome P-450 (CYP) may be involved in 1-BP-induced hepatotoxicity. The decreased content of hepatic GSH produced by 1-BP was associated not only with increased activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) but also with elevated levels of hepatic thiobarbituric acid-reactive substance (TBARS) in mice where metabolic enzymes were induced by pretreatment with phenobarbital. In addition, the hepatotoxicity induced by 1-BP was prevented by pretreatment with SKF-525A. Taken together, the formation of reactive metabolites by CYP and depletion of GS…

Jaime Wisniak – One of the best experts on this subject based on the ideXlab platform.

  • Phase Equilibria in the Systems Ethyl Methanoate + 1-Bromopropane, Ethyl Methanoate + Cyclohexane, and Ethyl Methanoate + 1-Bromopropane + Cyclohexane
    Journal of Chemical & Engineering Data, 1996
    Co-Authors: Jaime Wisniak

    Abstract:

    Vapor−liquid equilibrium at 101.3 kPa has been determined for the binary systems ethyl methanoate + 1-Bromopropane and ethyl methanoate + cyclohexane and the ternary system ethyl methanoate + 1-Bromopropane + cyclohexane. The two binary systems exhibit positive deviations from ideality and the binary ethyl methanoate + cyclohexane has an azeotrope that boils at 325.9 K and contains 82.2 mol % ethyl methanoate. The data were correlated by the Redlich−Kister, Wilson, NRTL, UNIQUAC, and Wisniak−Tamir equations, and the appropriate parameters are reported. The activity coefficients of the ternary system can be predicted from those of the pertinent binary systems. No ternary azeotrope is present.

  • Isobaric vapor-liquid equilibria in the binary systems of 1-Bromopropane with cyclohexane, heptane, and 1-butanol
    Journal of Chemical & Engineering Data, 1995
    Co-Authors: Jaime Wisniak, Alex Apelblat, Jacob Zabicky, Ina Feingold

    Abstract:

    Vapor-liquid equilibrium at 101.3 kPa has been determined for the binary systems of 1-Bromopropane with cyclohexane, heptane, and 1-butanol. The three systems exhibit positive deviations from ideal behavior. The activity coefficients and boiling point of the solution were correlated with its composition by the Redlich-Kister, Van Laar, and Wisniak-Tamir equations

  • Vapor-liquid equilibria in the ternary system 1-Bromopropane + 1-chlorobutane + cyclohexane
    Thermochimica Acta, 1995
    Co-Authors: Jaime Wisniak

    Abstract:

    The vapor-liquid equilibrium at 101.3 kPa has been determined for the ternary system 1-Bromopropane + 1-chlorobutane + cyclohexane. The data were correlated by the Redlich-Kister and Wisniak-Tamir equations and the appropriate parameters are reported. The activity coefficients of the ternary system can be predicted from those of the pertinent binary systems. No ternary azeotrope is present.