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9-Cis-Epoxycarotenoid Dioxygenase

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Ping Leng – 1st expert on this subject based on the ideXlab platform

  • Expression analysis of the DkNCED1, DkNCED2 and DkCYP707A1 genes that regulate homeostasis of abscisic acid during the maturation of persimmon fruit
    Journal of Horticultural Science & Biotechnology, 2020
    Co-Authors: Shengli Zhao, Yanping Wang, Kai Ji, Jian-xun Qi, Chaorui Duan, Pei Chen, Ping Leng

    Abstract:

    SummaryTo reveal details of the role(s) of abscisic acid (ABA) during fruit ripening and the regulation of ABA levels, two 9-Cis-Epoxycarotenoid Dioxygenase genes (DkNCED1 and DkNCED2) and the DkCYP707A1 gene for ABA 8′-hydroxylase were isolated from unripened persimmon fruit. Expression of DkNCED1 increased at the start of fruit maturation and peaked 20 d before harvest, which was consistent with the accumulation of ABA during ripening in persimmon. Expression of the DkNCED2 gene was high in fruit at the immature stage, then declined continuously throughout ripening. Compared to the DkNCED genes, expression of DkCYP707A1 peaked 145 d after full-bloom (DAFB), then declined. Ethylene release decreased gradually from 65 DAFB to 115 DAFB, then peaked at 10 d after harvest. Water-deficit stress enhanced the expression of DkNCED1, but down-regulated transcript levels of DkCYP707A1 in the calyx. These results suggest that endogenous ABA content is modulated by a dynamic balance between ABA biosynthesis and cata…

  • functional analysis of slnced1 in pistil development and fruit set in tomato solanum lycopersicum l
    Scientific Reports, 2019
    Co-Authors: Ying Fu, Bin Liang, Qian Li, Juan Wang, Ping Leng

    Abstract:

    Abscisic acid (ABA) is an important regulator of many plant developmental processes, although its regulation in the pistil during anthesis is unclear. We investigated the role of 9-Cis-Epoxycarotenoid Dioxygenase (SlNCED1), a key ABA biosynthesis enzyme, through overexpression and transcriptome analysis in the tomato pistil. During pistil development, ABA accumulates and SlNCED1 expression increases continually, peaking one day before full bloom, when the maximum amount of ethylene is released in the pistil. ABA accumulation and SlNCED1 expression in the ovary remained high for three days before and after full bloom, but then both declined rapidly four days after full bloom following senescence and petal abscission and expansion of the young fruits. Overexpression of SlNCED1 significantly increased ABA levels and also up-regulated SlPP2C5 expression, which reduced ABA signaling activity. Overexpression of SlNCED1 caused up-regulation of pistil-specific Zinc finger transcription factor genes SlC3H29, SlC3H66, and SlC3HC4, which may have affected the expression of SlNCED1-mediated pistil development-related genes, causing major changes in ovary development. Increased ABA levels are due to SlNCED1 overexpresson which caused a hormonal imbalance resulting in the growth of parthenocarpic fruit. Our results indicate that SlNCED1 plays a crucial role in the regulation of ovary/pistil development and fruit set.

  • Role of abscisic acid in regulating fruit set and ripening in squash (Cucurbita pepo L.)
    New Zealand Journal of Crop and Horticultural Science, 2016
    Co-Authors: Pengfei Chen, Bin Liang, Yushu Zhang, Xiawan Zhai, Suihuan He, Ping Leng

    Abstract:

    ABSTRACTSquash is a non-climacteric fruit, so the release of ethylene plays a limited role in the ripening process; however, there is a large accumulation of abscisic acid (ABA) during fruit maturation and ripening. To investigate the contribution of ABA in squash fruit development, the CpNCED1 gene, which encodes the key enzyme in ABA biosynthesis (9-Cis-Epoxycarotenoid Dioxygenase [NCED]), was cloned from squash fruit, and its transcriptional regulation during fruit development, dehydration and pollination was analysed using a quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression of CpNCED1 peaked 20 days after full bloom (DAFB) in the pulp and seed, and 25 DAFB in the peel, all of which corresponded with the accumulation of ABA. The application of exogenous ABA increased the expression of CpNCED1 and the release of ethylene, thereby promoting fruit ripening. The expression of CpNCED1 was also induced by dehydration stress and pollination. These findings show that ABA pl…

Suzanne R Abrams – 2nd expert on this subject based on the ideXlab platform

  • Sesquiterpene-like inhibitors of a 9-Cis-Epoxycarotenoid Dioxygenase regulating abscisic acid biosynthesis in higher plants
    Bioorganic and Medicinal Chemistry, 2009
    Co-Authors: Jason Boyd, Yuanzhu Gai, Ken M. Nelson, Erica Lukiwski, James Talbot, Mary K. Loewen, Stacey Owen, L. Irina Zaharia, Adrian J. Cutler, Suzanne R Abrams

    Abstract:

    Abscisic acid (ABA) is a carotenoid-derived plant hormone known to regulate critical functions in growth, development and responses to environmental stress. The key enzyme which carries out the first committed step in ABA biosynthesis is the carotenoid cleavage 9-Cis-Epoxycarotenoid Dioxygenase (NCED). We have developed a series of sulfur and nitrogen-containing compounds as potential ABA biosynthesis inhibitors of the NCED, based on modification of the sesquiterpenoid segment of the 9-cis-xanthophyll substrates and product. In in vitro assays, three sesquiterpene-like carotenoid cleavage Dioxygenase (SLCCD) inhibitor compounds 13, 17 and 18 were found to act as inhibitors of Arabidopsis thaliana NCED 3 (AtNCED3) with Ki′s of 93, 57 and 87 μM, respectively. Computational docking to a model of AtNCED3 supports a mechanism of inhibition through coordination of the heteroatom with the non-heme iron in the enzyme active site. In pilot studies, pretreatment of osmotically stressed Arabidopsis plants with compound 13 resulted lower levels of ABA and catabolite accumulation compared to levels in mannitol-stressed plant controls. This same inhibitor moderated known ABA-induced gene regulation effects and was only weakly active in inhibition of seed germination. Interestingly, all three inhibitors led to moderation of the stress-induced transcription of AtNCED3 itself, which could further contribute to lowering ABA biosynthesis in planta. Overall, these sesquiterpenoid-like inhibitors present new tools for controlling and investigating ABA biosynthesis and regulation. Crown Copyright © 2009.

Kazuo Shinozaki – 3rd expert on this subject based on the ideXlab platform

  • N-Benzylideneaniline and N-Benzylaniline are Potent Inhibitors of Lignostilbene-α,β-Dioxygenase, a Key Enzyme in Oxidative Cleavage of the Central Double Bond of Lignostilbene
    Journal of Enzyme Inhibition and Medicinal Chemistry, 2020
    Co-Authors: Hiroki Inoue, Kazuo Shinozaki, Masatomo Kobayashi, Tamio Saito, Katsuhiko Sekimata, Tamami Terada, Shigehiro Kamoda, Yoshimasa Saburi, Shigeo Yoshida

    Abstract:

    Lignostilbene-α,β-Dioxygenase (LSD, EC 1.13.11.43) is involved in oxidative cleavage of the central double bond of lignostilbene to form the corresponding aldehydes by a mechanism similar to those of 9-Cis-Epoxycarotenoid Dioxygenase and β-carotene 15,15′-Dioxygenase, key enzymes in abscisic acid biosynthesis and vitamin A biosynthesis, respectively. In this study, several N-benzylideneanilines and amine were synthesized and examined for their efficacy as inhibitors of LSD. N-(4-Hydroxybenzylidene)-3-methoxyaniline was found to be a potent inhibitor with IC50 = 0.3 µM and N-(4-hydroxybenzyl)-3-methoxyaniline was also active with IC50 = 10 µM. The information obtained from the structure-activity relationships study here can aid in discovering inhibitors of both abscisic acid and vitamin A biosynthesis.

  • Characterization of the promoter region of an Arabidopsis gene for 9-Cis-Epoxycarotenoid Dioxygenase involved in dehydration-inducible transcription.
    DNA Research, 2013
    Co-Authors: Babak Behnam, Satoshi Iuchi, Masatomo Kobayashi, Kazuko Yamaguchi-shinozaki, Miki Fujita, Yasunari Fujita, Hironori Takasaki, Yuriko Osakabe, Kazuo Shinozaki

    Abstract:

    Plants respond to dehydration stress and tolerate water-deficit status through complex physiological and cellular processes. Many genes are induced by water deficit. Abscisic acid (ABA) plays important roles in tolerance to dehydration stress by inducing many stress genes. ABA is synthesized de novo in response to dehydration. Most of the genes involved in ABA biosynthesis have been identified, and they are expressed mainly in leaf vascular tissues. Of the products of such genes, 9-Cis-Epoxycarotenoid Dioxygenase (NCED) is a key enzyme in ABA biosynthesis. One of the five NCED genes in Arabidopsis, AtNCED3, is significantly induced by dehydration. To understand the regulatory mechanism of the early stages of the dehydration stress response, it is important to analyse the transcriptional regulatory systems of AtNCED3. In the present study, we found that an overlapping G-box recognition sequence (5′-CACGTG-3′) at −2248 bp from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response. We discuss the possible transcriptional regulatory system of dehydration-responsive AtNCED3 expression, and how this may control the level of ABA under water-deficit conditions.

  • Drought Induction of Arabidopsis 9-Cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells
    Plant Physiology, 2008
    Co-Authors: Akira Endo, Yoshiaki Sawada, Hirokazu Takahashi, Masanori Okamoto, Keiichi Ikegami, Hanae Koiwai, Tomonobu Toyomasu, Wataru Mitsuhashi, Kazuo Shinozaki

    Abstract:

    The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In this study, we examined the tissue-specific localization of ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis (Arabidopsis thaliana) plants using specific antibodies against 9-Cis-Epoxycarotenoid Dioxygenase 3 (AtNCED3), AtABA2, and Arabidopsis aldehyde oxidase 3 (AAO3). Immunohistochemical analysis revealed that in turgid plants, AtABA2 and AAO3 proteins were localized in vascular parenchyma cells most abundantly at the boundary between xylem and phloem bundles, but the AtNCED3 protein was undetectable in these tissues. In water-stressed plants, AtNCED3 was detected exclusively in the vascular parenchyma cells together with AtABA2 and AAO3. In situ hybridization using the antisense probe for AtNCED3 showed that the drought-induced expression of AtNCED3 was also restricted to the vascular tissues. Expression analysis of laser-microdissected cells revealed that, among nine drought-inducible genes examined, the early induction of most genes was spatially restricted to vascular cells at 1 h and then some spread to mesophyll cells at 3 h. The spatial constraint of AtNCED3 expression in vascular tissues provides a novel insight into plant systemic response to drought stresses.