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López, Alicia Noemí - One of the best experts on this subject based on the ideXlab platform.

  • Effects of chlorpyrifos, cypermethrin, and glyphosate on Milax gagates (Mollusca: Pulmonata) and Armadillidium Vulgare (Crustacea: Isopoda)
    2015
    Co-Authors: Salvio Carla, Manetti Pablo, Clemente Natalia, López, Alicia Noemí
    Abstract:

    El objetivo fue evaluar el efecto tóxico de Clorpirifos, Cipermetrina y Glifosato sobre Milax gagates y Armadillidium Vulgare. La unidad experimental fue un recipiente (36, 5 x 24,5 cm) con 10 cm de suelo, 45 g de residuo vegetal y 10 individuos de M. gagates (400-600 mg) o de A. Vulgare (60-80 mg). Los tratamientos fueron: 0, 120, 240, 480, 960 y 1440 g de Clorpirifos ha (48% de ingrediente activo (i.a.)); 0, 12,5, 25, 50, 75 y 100 g de -1 Cipermetrina ha (25% de i.a.) y 0, 480, 960, 1440, 1920 y 2400 -1 g de Glifosato ha (48% de i.a.). Para cada bioensayo se utilizó un diseño completamente aleatorizado con 4 repeticiones y se llevó a cabo en cámara a 20+2ºC y 14L:10O. Se contaron individuos vivos y muertos a las 24, 48, 72, 96 y 120 horas después de la aplicación (HDA). Se analizó la proporción de individuos muertos mediante modelos lineales generalizados y las dosis de cada plaguicida se compararon mediante la prueba de mínima diferencia significativa (α=0,05). Los tres plaguicidas a sus distintas dosis no causaron mortalidad sobre M. gagates. La proporción de A. Vulgare muertos difirió entre las dosis de Clorpirifos y de Cipermetrina (p< 0,05) y fueron superiores a 78%. Con Glifosato no se observaron individuos muertos de A. Vulgare. Se concluye que los tres plaguicidas sobre M. gagates como Glifosato sobre A. Vulgare no causaron efectos perjudiciales, pero en cambio Clorpirifos y Cipermetrina a sus diferentes dosis ocasionaron mortalidad sobre A. Vulgare.The objective of this work was to evaluate the toxicity of Chlorpyrifos, Cypermethrin and Glyphosate on Milax gagates and Armadillidium Vulgare. The experimental unit was a container (36.5 x 24.5 cm) with 10 cm of soil, 45 g of plant residue and 10 individuals of M. gagates (400-600 mg) or A. Vulgare (60-80 mg). The treatments were: 0, 120, 240, 480, 960 and 1440 g Chlorpyrifos ha (48% active ingredient (a.i.)); 0, 12.5, 25, 50, -1 75 and 100 g Cypermethrin ha (25% a.i.); and 0, 480, 960, -1 1440, 1920 and 2400 g Glyphosate ha (48% a.i.). Bioassays were conducted in a chamber at 20+2°C and 14L:10O, using a completely randomized design with 4 replicates. Individuals alive and dead at 24, 48, 72, 96 and 120 hours after application were counted. The proportion of dead individuals was analyzed using generalized linear models, and doses were compared using the least significant difference test (α = 0.05). No mortality of M. gagates was recorded with any pesticide. The proportion of dead A. Vulgare differed between Chlorpyrifos and Cypermethrin doses (p

  • Effects of Carbaryl and Metaldehyde on Armadillidium Vulgare (Crustacea: Isopoda) and Milax gagates (Mollusca: Pulmonata) in soybean under no-tillage
    2014
    Co-Authors: Salvio Carla, Manetti Pablo, Clemente Natalia, López, Alicia Noemí
    Abstract:

    Los objetivos fueron evaluar la eficacia de control y las dosis de los cebos Metaldehído 4% + Carbaryl 8% (2, 4 y 6 kg ha-1), Carbaryl 8 %, y Metaldehído 4 % y 5 % (2 y 4 kg ha-1) sobre Armadillidium Vulgare y Milax gagates y determinar el número de plantas y rendimiento en soja. El diseño fue en bloques completos aleatorizados con tres repeticiones. Se evaluó el número de A. Vulgare y M. gagates vivos a los 3, 7, 10 y 16 días después de la aplicación de los cebos (DDA), número de plantas m-2 y rendimiento (kg ha-1). El número de A. Vulgare fue menor con Metaldehído 4 % + Carbaryl 8 % y con los cebos con Carbaryl 8 % respecto al testigo a los 3, 7, 10 y 16 DDA (χ2 ptrat < 0,05). A los 3 DDA, el número de M. gagates disminuyó con Metaldehído 4 % + Carbaryl 8 % y Metaldehído 4 % y 5 % en relación al testigo (χ2 ptrat = 0,0806). El número de plantas m-2 difirió entre los tratamientos (χ2 ptrat = 0,0003), y se obtuvo un número mayor con la aplicación de los cebos que con el testigo. En el rendimiento se detectó efecto de los tratamientos (χ2 ptrat = 0,0188), sin embargo sólo la dosis de 2 kg ha-1 de Carbaryl 8 % fue diferente del testigo. Se concluye que la eficacia de control del cebo Metaldehído + Carbaryl resultó semejante a la obtenida con los crustacicidas formulados con Carbaryl sobre A. Vulgare y a los molusquicidas con Metaldehído sobre M. gagates. La aplicación de los cebos aumentó el número de plantas.The aim was to evaluate the control efficacy and dose of Metaldehyde 4 % + Carbaryl 8 % (2, 4 y 6 kg ha-1), Carbaryl 8 %, and Metaldehyde 4 % y 5 % (2 y 4 kg ha-1) baits on Armadillidium Vulgare and Milax gagates, and to determine the number of plants and yield in soybean. The design was a randomized complete block with three replications. We evaluated the number of A. Vulgare and M. gagates alive 3, 7, 10 and 16 days after application of bait (DAA), number of plants m-2, and yield (kg ha-1). The number of A. Vulgare was lower with Metaldehyde 4 % + Carbaryl 8 % and with baits based on Carbaryl 8 % compared to the control at 3, 7, 10 and 16 DAA (χ2 ptrat

  • Nivel de daño económico de Armadillidium Vulgare (Latreille, 1804) (Crustacea Isopoda) en el cultivo de girasol
    2014
    Co-Authors: Faberi, Ariel Jesús, Clemente, Natalia Liliana, Manetti, Pablo Luis, López, Alicia Noemí
    Abstract:

    La función de daño es el parámetro fundamental del modelo de Nivel de Daño Económico (NDE). Los objetivos fueron: i) evaluar los perjuicios de Armadillidium Vulgare y determinar la función de daño para poder estimar los NDE en el cultivo de girasol. Las evaluaciones se realizaron en tres ciclos agrícolas según un diseño en bloques completos aleatorizados con cinco repeticiones. Se sembraron ocho semillas de girasol y se infestaron con: 0(testigo), 20, 40, 60, 80 y 120 individuos m-2 de A. Vulgare en parcelas de 1m2. Se evaluaron los perjuicios en hipocótile y cotiledones, número de plantas m-2 y el rendimiento. Se calcularon las funciones de daño (regresión entre la densidad de A. Vulgare y la proporción de pérdida de rendimiento). Se observaron diferencias en la proporción de plantas con perjuicios severos en el hipocótile entre las densidades de A. Vulgare. Esta categoría de perjuicio se correlacionó con la densidad de A. Vulgare, con el número de plantas m-2 y con el rendimiento. Las funciones de daño representaron una proporción de pérdida de rendimiento de 0,0033 (R2= 0,64), 0,0023 (R2= 0,49) y 0,0048 (R2= 0,71) por individuo de A. Vulgare por metro cuadrado para los tres ciclos agrícolas, respectivamente. Cuando se combinaron todos los datos ésta fue de 0,0034 (R2= 0,59). Considerando valores económicos promedio, los NDE de A. Vulgare en el cultivo de girasol variaron entre 22 y 81 individuos m-2. Teniendo en cuenta los coeficientes de determinación y que A. Vulgare tiene hábitos alimenticios variables, la función de daño de 0,0033 es recomendable en el cálculo de NDE de A. Vulgare en girasol

  • Nivel de daño económico de Armadillidium Vulgare (Latreille, 1804) (Crustacea: Isopoda) en el cultivo de girasol
    Gerencia de Comunicación e Imagen Institucional DNA SICC INTA, 2014
    Co-Authors: Faberi Ariel, Manetti Pablo, Clemente Natalia, López, Alicia Noemí
    Abstract:

    La función de daño es el parámetro fundamental del modelo de Nivel de Daño Económico (NDE). Los objetivos fueron: evaluar los perjuicios de Armadillidium Vulgare en el cultivo de girasol y determinar la función de daño en el cultivo para poder estimar el NDE. Las evaluaciones se realizaron en tres ciclos agrícolas según un diseño en bloques completos aleatorizados con cinco repeticiones. Se sembraron ocho semillas de girasol y se infestaron con: 0 (testigo), 20, 40, 60, 80 y 120 individuos m-2 de A. Vulgare en parcelas de 1m2 . Se evaluaron los perjuicios en hipocótile y cotiledones, número de plantas m-2 y el rendimiento. Se calcularon las funciones de daño (regresión entre la densidad de A. Vulgare y la proporción de pérdida de rendimiento). Se observaron diferencias en la proporción de plantas con perjuicios severos en el hipocótile entre las densidades de A. Vulgare. Esta categoría de perjuicio se correlacionó con la densidad de A. Vulgare, con el número de plantas m-2 y con el rendimiento. Las funciones de daño representaron una proporción de pérdida de rendimiento de 0,0033 (R2 = 0,64), 0,0023 (R2 = 0,49) y 0,0048 (R2 = 0,71) por individuo por metro cuadrado para los tres ciclos agrícolas, respectivamente. Cuando se combinaron todos los datos ésta fue de 0,0034 (R2 = 0,59). Considerando valores económicos promedio, los NDE de A. Vulgare en el cultivo de girasol variaron entre 22 y 81 individuos m-2. Teniendo en cuenta los coeficientes de determinación y que A. Vulgare tiene hábitos alimenticios variables, la función de daño de 0,0033 es recomendable en el cálculo de NDE de A. Vulgare en girasol.Damage function is the parameter of Economic Injury Level model (EIL). The aims were: evaluate the injury of Armadillidium Vulgare in the sunflower crop and determine the damage function in the crop for estimating EIL. The study was done in three agricultural cycles. Assessments were conducted in three agricultural cycles according randomized complete blocks design with five replicates. Eight sunflower seeds were sown and were infested with: 0 (control), 20, 40, 60, 80 y 120 individuals m-2 of A. Vulgare in 1 m2 plot. Injury on hypocotil and cotiledons, number of plants per square meter and yield were evaluated. Damage functions were calculated (A. Vulgare density and yield loss proportion regression). Differences in the proportion of plants with severe injury were observed among A. Vulgare densities. This category of injury was correlated with A. Vulgare density, with the number of plant per square meter and with yield. Damage functions represented a proportion of yield loss of 0.0033 (R2 = 0.64), 0.0023 (R2 = 0.49) and 0.0048 (R2 = 0.71) for A. Vulgare individual by square meter in three agricultural cycles. When all data were combined this was 0.0035 (R2 = 0.59). Considering mean market values, the EIL of A. Vulgare in sunflower crop ranged from 22 to 81 individuals m-2. Taking into account the determination coefficients and that A. Vulgare has variable eating habits the 0.0033 damage function is recommended to determine EIL of A. Vulgare in sunflower crop.EEA BalcarceFil: Faberi, Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Unidad Integrada Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Clemente, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Unidad Integrada Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Manetti, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Unidad Integrada Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Lopez, A.N. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Unidad Integrada Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentin

Yves Le Bigot - One of the best experts on this subject based on the ideXlab platform.

  • Genome sequence of a crustacean iridovirus, IIV31, isolated from the pill bug, Armadillidium Vulgare.
    Journal of General Virology, 2014
    Co-Authors: Benoit Piegu, Sebastien Guizard, Brian A. Federici, Corinne Cruaud, Dennis K Bideshi, Tan Yeping, Yves Le Bigot
    Abstract:

    Members of the family Iridoviridae are animal viruses that infect only invertebrates and poikilothermic vertebrates. The invertebrate iridovirus 31 (IIV31) was originally isolated from adult pill bugs, Armadillidium Vulgare (class Crustacea, order Isopoda, suborder Oniscidea), found in southern California on the campus of the University of California, Riverside, USA. IIV31 virions are icosahedral, have a diameter of about 135 nm, and contain a dsDNA genome 220.222 kbp in length, with 35.09 mol % G+C content and 203 ORFs. Here, we describe the complete genome sequence of this virus and its annotation. This is the eighth genome sequence of an IIV reported.

  • Genome sequence of a crustacean iridovirus, IIV31, isolated from the pill bug, [i]Armadillidium Vulgare[/i]
    Journal of General Virology, 2014
    Co-Authors: Benoit Piegu, Sebastien Guizard, Brian A. Federici, Corinne Cruaud, Dennis K Bideshi, Tan Yeping, Yves Le Bigot
    Abstract:

    Members of the family Iridoviridae are animal viruses that infect only invertebrates and poikilothermic vertebrates. The invertebrate iridovirus 31 (IIV31) was originally isolated from adult pill bugs, Armadillidium Vulgare (class Crustacea, order Isopoda, suborder Oniscidea), found in southern California on the campus of the University of California, Riverside, USA. IIV31 virions are icosahedral, have a diameter of about 135 nm, and contain a dsDNA genome 220.222 kbp in length, with 35.09 mol % G+C content and 203 ORFs. Here, we describe the complete genome sequence of this virus and its annotation. This is the eighth genome sequence of an IIV reported.

Didier Bouchon - One of the best experts on this subject based on the ideXlab platform.

  • Feminizing Wolbachia influence microbiota composition in the terrestrial isopod Armadillidium Vulgare
    Scientific Reports, 2018
    Co-Authors: Jessica Dittmer, Didier Bouchon
    Abstract:

    Wolbachia are widespread heritable endosymbionts of arthropods notorious for their profound effects on host fitness as well as for providing protection against viruses and eukaryotic parasites, indicating that they can interact with other microorganisms sharing the same host environment. Using the terrestrial isopod crustacean Armadillidium Vulgare, its highly diverse microbiota (>200 bacterial genera) and its three feminizing Wolbachia strains (wVulC, wVulM, wVulP) as a model system, the present study demonstrates that Wolbachia can even influence the composition of a diverse bacterial community under both laboratory and natural conditions. While host origin is the major determinant of the taxonomic composition of the microbiota in A. Vulgare, Wolbachia infection affected both the presence and, more importantly, the abundance of many bacterial taxa within each host population, possibly due to competitive interactions. Moreover, different Wolbachia strains had different impacts on microbiota composition. As such, infection with wVulC affected a higher number of taxa than infection with wVulM, possibly due to intrinsic differences in virulence and titer between these two strains. In conclusion, this study shows that heritable endosymbionts such as Wolbachia can act as biotic factors shaping the microbiota of arthropods, with as yet unknown consequences on host fitness.

  • Armadillidin H, a glycine-rich peptide from the terrestrial crustacean Armadillidium Vulgare, displays an unexpected wide antimicrobial spectrum with membranolytic activity.
    Frontiers Media S.A., 2016
    Co-Authors: Julien Verdon, Pierre Greve, Bouziane Moumen, Coutos-thevenot Pierre, Marie-helene Rodier, Celine Landon, Segolene Depayras, Cyril Noel, Sylvain La Camera, Didier Bouchon
    Abstract:

    Antimicrobial peptides (AMPs) are key components of innate immunity and are widespread in nature, from bacteria to vertebrate animals. In crustaceans, there are currently 15 distinct AMP families published so far in the literature, mainly isolated from members of the Decapoda order. Up to now, armadillidin is the sole non-decapod AMP isolated from the haemocytes of Armadillidium Vulgare, a crustacean isopod. Its first description demonstrated that armadillidin is a linear glycine-rich (47%) cationic peptide with an antimicrobial activity directed towards Bacillus megaterium. In the present work, we report identification of armadillidin Q, a variant of armadillidin H (earlier known as armadillidin), from crude haemocyte extracts of A. Vulgare using LC-MS approach. We demonstrated that both armadillidins displayed broad spectrum antimicrobial activity against several Gram-positive and Gram negative bacteria, fungi, but were totally inactive against yeasts. Membrane permeabilization assays, only performed with armadillidin H, showed that the peptide is membrane active against bacterial and fungal strains leading to deep changes in cell morphology. This damaging activity visualized by electronic microscopy correlates with a rapid decrease of cell viability leading to highly blebbed cells. In contrast, armadillidin H does not reveal cytotoxicity towards human erythrocytes. Furthermore, no secondary structure could be defined in this study (by CD and NMR) even in a membrane mimicking environment. Therefore, armadillidins represent interesting candidates to gain insight into the biology of glycine-rich AMPs

  • Large gene overlaps and tRNA processing in the compact mitochondrial genome of the crustacean Armadillidium Vulgare.
    RNA Biology, 2015
    Co-Authors: Vincent Doublet, Didier Bouchon, Isabelle Marcade, Elodie Ubrig, Abdelmalek Alioua, Laurence Maréchal-drouard
    Abstract:

    A faithful expression of the mitochondrial DNA is crucial for cell survival. Animal mitochondrial DNA (mtDNA) presents a highly compact gene organization. The typical 16.5 kbp animal mtDNA encodes 13 proteins, 2 rRNAs and 22 tRNAs. In the backyard pillbug Armadillidium Vulgare, the rather small 13.9 kbp mtDNA encodes the same set of proteins and rRNAs as compared to animal kingdom mtDNA, but seems to harbor an incomplete set of tRNA genes. Here, we first confirm the expression of 13 tRNA genes in this mtDNA. Then we show the extensive repair of a truncated tRNA, the expression of tRNA involved in large gene overlaps and of tRNA genes partially or fully integrated within protein-coding genes in either direct or opposite orientation. Under selective pressure, overlaps between genes have been likely favored for strong genome size reduction. Our study underlines the existence of unknown biochemical mechanisms for the complete gene expression of A. Vulgare mtDNA, and of co-evolutionary processes to keep overlapping genes functional in a compacted mitochondrial genome.

  • the immune cellular effectors of terrestrial isopod Armadillidium Vulgare meeting with their invaders wolbachia
    PLOS ONE, 2011
    Co-Authors: Frederic D Chevalier, Pierre Greve, Maryline Raimond, Didier Bouchon, Juline Herbinieregaboreau, Joanne Bertaux, Franck Morel, Christine Braquartvarnier
    Abstract:

    Background Most of crustacean immune responses are well described for the aquatic forms whereas almost nothing is known for the isopods that evolved a terrestrial lifestyle. The latter are also infected at a high prevalence with Wolbachia, an endosymbiotic bacterium which affects the host immune system, possibly to improve its transmission. In contrast with insect models, the isopod Armadillidium Vulgare is known to harbor Wolbachia inside the haemocytes. Methodology/Principal Findings In A. Vulgare we characterized three haemocyte types (TEM, flow cytometry): the hyaline and semi-granular haemocytes were phagocytes, while semi-granular and granular haemocytes performed encapsulation. They were produced in the haematopoietic organs, from central stem cells, maturing as they moved toward the edge (TEM). In infected individuals, live Wolbachia (FISH) colonized 38% of the haemocytes but with low, variable densities (6.45±0.46 Wolbachia on average). So far they were not found in hyaline haemocytes (TEM). The haematopoietic organs contained 7.6±0.7×103 Wolbachia, both in stem cells and differentiating cells (FISH). While infected and uninfected one-year-old individuals had the same haemocyte density, in infected animals the proportion of granular haemocytes in particular decreased by one third (flow cytometry, Pearson's test = 12 822.98, df = 2, p<0.001). Conclusions/Significance The characteristics of the isopod immune system fell within the range of those known from aquatic crustaceans. The colonization of the haemocytes by Wolbachia seemed to stand from the haematopoietic organs, which may act as a reservoir to discharge Wolbachia in the haemolymph, a known route for horizontal transfer. Wolbachia infection did not affect the haemocyte density, but the quantity of granular haemocytes decreased by one third. This may account for the reduced prophenoloxidase activity observed previously in these animals.

  • evidence for a new feminizing wolbachia strain in the isopod Armadillidium Vulgare evolutionary implications
    Heredity, 2004
    Co-Authors: Richard Cordaux, Thierry Rigaud, A Michelsalzat, M Frelonraimond, Didier Bouchon
    Abstract:

    Wolbachia are intracellular maternally inherited alpha-Proteobacteria infecting a wide range of arthropods. In the common pill bug Armadillidium Vulgare, the known Wolbachia strain is responsible for feminization of genetic males. We have investigated Wolbachia diversity in 20 populations of A. Vulgare from west and east Europe, north Africa and north America. A new Wolbachia strain (wVulM) was identified through the variability of the wsp gene, distantly related to that previously known (wVulC) in this host species. No individual with multiple infections was detected. Inoculation experiments indicated that the new wVulM bacterial strain also induces feminization in A. Vulgare. However, the wVulC strain showed a higher transmission rate than the wVulM strain and was the most geographically widespread Wolbachia in A. Vulgare populations. Mitochondrial 16SrDNA gene sequencing was conducted in Wolbachia-infected individuals, revealing the occurrence of four host lineages. The comparison of bacterial strains and their respective host mitochondrial phylogenies failed to show concordance, indicating horizontal transmission of the Wolbachia strains within populations of A. Vulgare.

Adrian Gh. Podoleanu - One of the best experts on this subject based on the ideXlab platform.

Richard Cordaux - One of the best experts on this subject based on the ideXlab platform.

  • fine scale population structure analysis in Armadillidium Vulgare isopoda oniscidea reveals strong female philopatry
    Acta Oecologica-international Journal of Ecology, 2019
    Co-Authors: Sylvine Durand, Isabelle Giraud, Frédéric Grandjean, Richard Cordaux, Sophie Beltranbech, Nicolas Bech
    Abstract:

    Abstract In the last decades, dispersal studies have benefited from the use of molecular markers for detecting patterns differing between categories of individuals and have highlighted sex-biased dispersal in several species. To explain this phenomenon, several hypotheses implying mating systems, intrasexual competition or sex-related handicaps have been proposed. In this context, we investigated sex-biased dispersal in Armadillidium Vulgare, a terrestrial isopod with a promiscuous mating system. As a proxy for effective dispersal, we performed a fine-scale investigation of the spatial genetic structure in males and females, using individuals originating from five sampling points located within 70 m of each other. Based on microsatellite markers and spatial autocorrelation analyses, our results revealed that while males did not present a significant genetic structure at this geographic scale, females were significantly and genetically more similar to each other when they were collected in the same sampling point. As females invest more parental care than males in A. Vulgare, but also because this species is promiscuous and males experience a high intrasexual competition, our results meet the predictions of most classical hypotheses for sex-biased dispersal. We suggest that widening dispersal studies to other isopods or crustaceans, differing in their ecology or mating system and displaying varying levels of parental care, might shed light on the processes underlying the evolution of sex-biased dispersal.

  • the genome of Armadillidium Vulgare crustacea isopoda provides insights into sex chromosome evolution in the context of cytoplasmic sex determination
    Molecular Biology and Evolution, 2019
    Co-Authors: Mohamed Amine Chebbi, Thomas Becking, Isabelle Giraud, Bouziane Moumen, Clement Gilbert, Jean Peccoud, Richard Cordaux
    Abstract:

    The terrestrial isopod Armadillidium Vulgare is an original model to study the evolution of sex determination and symbiosis in animals. Its sex can be determined by ZW sex chromosomes, or by feminizing Wolbachia bacterial endosymbionts. Here, we report the sequence and analysis of the ZW female genome of A. Vulgare. A distinguishing feature of the 1.72 gigabase assembly is the abundance of repeats (68% of the genome). We show that the Z and W sex chromosomes are essentially undifferentiated at the molecular level and the W-specific region is extremely small (at most several hundreds of kilobases). Our results suggest that recombination suppression has not spread very far from the sex-determining locus, if at all. This is consistent with A. Vulgare possessing evolutionarily young sex chromosomes. We characterized multiple Wolbachia nuclear inserts in the A. Vulgare genome, none of which is associated with the W-specific region. We also identified several candidate genes that may be involved in the sex determination or sexual differentiation pathways. The A. Vulgare genome serves as a resource for studying the biology and evolution of crustaceans, one of the most speciose and emblematic metazoan groups.

  • Evolutionary Significance of Wolbachia-to-Animal Horizontal Gene Transfer: Female Sex Determination and the f Element in the Isopod Armadillidium Vulgare.
    Genes, 2017
    Co-Authors: Richard Cordaux, Clement Gilbert
    Abstract:

    An increasing number of horizontal gene transfer (HGT) events from bacteria to animals have been reported in the past years, many of which involve Wolbachia bacterial endosymbionts and their invertebrate hosts. Most transferred Wolbachia genes are neutrally-evolving fossils embedded in host genomes. A remarkable case of Wolbachia HGT for which a clear evolutionary significance has been demonstrated is the "f element", a nuclear Wolbachia insert involved in female sex determination in the terrestrial isopod Armadillidium Vulgare. The f element represents an instance of bacteria-to-animal HGT that has occurred so recently that it was possible to infer the donor (feminizing Wolbachia closely related to the wVulC Wolbachia strain of A. Vulgare) and the mechanism of integration (a nearly complete genome inserted by micro-homology-mediated recombination). In this review, we summarize our current knowledge of the f element and discuss arising perspectives regarding female sex determination, unstable inheritance, population dynamics and the molecular evolution of the f element. Overall, the f element unifies three major areas in evolutionary biology: symbiosis, HGT and sex determination. Its characterization highlights the tremendous impact sex ratio distorters can have on the evolution of sex determination mechanisms and sex chromosomes in animals and plants.

  • Additional file 9: Table S3. of Comparative paleovirological analysis of crustaceans identifies multiple widespread viral groups
    2015
    Co-Authors: Gabriel Metegnier, Thomas Becking, Isabelle Giraud, Richard Cordaux, Bouziane Moumen, Mohamed Chebbi, Sarah Schaack, ClĂŠment Gilbert
    Abstract:

    PCR primers used to confirm the presence of EVEs uncovered by the bio-informatic analysis and to screen for orthologous insertions in 3 Armadillidae species : Armadillidium Vulgare, A. tunisiense and A. depressum. (DOCX 17 kb

  • Microsatellite multiplex kits developed for the terrestrial isopod Armadillidium Vulgare.
    2013
    Co-Authors: Isabelle Giraud, Victorien Valette, Nicolas Bech, Frédéric Grandjean, Richard Cordaux
    Abstract:

    Microsatellite multiplex kits developed for the terrestrial isopod Armadillidium Vulgare.