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Dibyendu Kamilya - One of the best experts on this subject based on the ideXlab platform.

  • Modulation of systemic and mucosal immune responses of Catla Catla (Hamilton, 1822) experimentally challenged with gill monogeneans
    Fish & shellfish immunology, 2018
    Co-Authors: Narinder Kaur, Rahul Kumar, Dibyendu Kamilya
    Abstract:

    Abstract The present study investigated the modulation of systemic and mucosal immunity of Catla (Catla Catla) experimentally challenged with gill monogeneans. Fingerlings of Catla (24.2 ± 1.5 g) without any parasites were randomly distributed into two groups each comprising quadruplicate tanks (10 fish per tank). The fish in the infected group were challenged with gill monogeneans by cohabitation method. The control group fish were not exposed to parasites. Serum and mucus were collected from both control and infected fish and different immunological parameters were measured at weekly interval for four weeks. Monogeneans on the fish gills were also quantified from infected fish at weekly interval. Different systemic immunological parameters viz. myeloperoxidase content, alkaline phosphatase and hemolytic activity of serum showed a significant increase (p

  • Susceptibility and pathological consequences of Catla, Catla Catla (Hamilton) experimentally infected with Edwardsiella tarda
    Archives of Polish Fisheries, 2016
    Co-Authors: Thongam Bidya Devi, T. Jawahar Abraham, Dibyendu Kamilya
    Abstract:

    AbstractThe present study tested the susceptibility and pathological changes of Catla, Catla Catla (Hamilton) infected with Edwardsiella tarda (ET-PG-29). The bacterium was isolated from the kidney of a diseased pangas catfish. To determine the median lethal dose (LD50), C. Catla were challenged with this bacterium (108-103CFU ml-1), and the LD50was calculated as 105.5CFU ml-1. Another set of healthy C. Catla were injected intraperitoneally with the LD50dose to induce edwardsiellosis. The clinical signs of the infected C. Catla were observed and recorded. Tissues such as kidney, liver, intestine, heart, and gill from the infected fish with clinical signs of edwardsiellosis were used for histopathology. The clinical and gross signs were first visible at 1 d post-injection, and the infected fish showed typical signs of hemorrhagic septicemia. The most striking histopathological features were found in the kidney which showed multi-focal necrosis with the formation of granuloma indicating an inflammatory response against the pathogen. The intestine displayed goblet cell hyperplasia, the liver showed hydropic degeneration with hyperemic central veins, and there was inflammation of gill lamellae and cardiac myositis associated with leucocyte infiltration. Collectively, the results confirmed the susceptibility of C. Catla to E. tarda infection and that this bacterium is a threat to C. Catla in aquaculture practices.

  • In Vitro and Dietary Effects of Chitin on Cellular and Humoral Immune Parameters of Catla, Catla Catla (Hamilton)
    Journal of the World Aquaculture Society, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Immunostimulants increase the immunocompetency and disease resistance of fish by enhancing immune responses. This study determined the in vitro and dietary effects of chitin on immune responses of Catla, Catla Catla. Catla head-kidney leukocytes were cultured in complete RPMI-1640 medium containing 0.01, 0.1, and 1.0 mg/mL of chitin. Leucocyte viability and different cellular immune responses, namely, respiratory burst, nitric oxide production, and leucocyte peroxidase content were analyzed in vitro. In the dietary experiment, chitin-supplemented diets (0.5, 1.0, and 2.0% of feed) were fed to Catla for 2 wk, and respiratory burst, serum lysozyme, and total protein contents were measured after 1 and 2 wk of feeding. Leucocytes incubated in vitro with chitin for 72 h maintained their viability. Chitin significantly (P 

  • in vitro and dietary effects of chitin on cellular and humoral immune parameters of Catla Catla Catla hamilton
    Journal of The World Aquaculture Society, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Immunostimulants increase the immunocompetency and disease resistance of fish by enhancing immune responses. This study determined the in vitro and dietary effects of chitin on immune responses of Catla, Catla Catla. Catla head-kidney leukocytes were cultured in complete RPMI-1640 medium containing 0.01, 0.1, and 1.0 mg/mL of chitin. Leucocyte viability and different cellular immune responses, namely, respiratory burst, nitric oxide production, and leucocyte peroxidase content were analyzed in vitro. In the dietary experiment, chitin-supplemented diets (0.5, 1.0, and 2.0% of feed) were fed to Catla for 2 wk, and respiratory burst, serum lysozyme, and total protein contents were measured after 1 and 2 wk of feeding. Leucocytes incubated in vitro with chitin for 72 h maintained their viability. Chitin significantly (P < 0.05) enhanced all the immune responses in vitro. The lowest dose showed the best response. Dietary chitin also significantly stimulated all the immune parameters in a dose-dependent manner with the highest dose showing the maximum responses. All the responses were higher after 1 wk of feeding and declined thereafter. The results suggest that chitin stimulates Catla cellular and humoral immune responses in vitro and in vivo.

  • dietary bacillus subtilis fptb13 and chitin single or combined modulate systemic and cutaneous mucosal immunity and resistance of Catla Catla Catla hamilton against edwardsiellosis
    Comparative Immunology Microbiology and Infectious Diseases, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Effects of dietary administration of Bacillus subtilis FPTB13 and chitin, single or combined, on the systemic immunity, mucosal immunity and resistance of Catla (Catla Catla) against Edwardsiella tarda infection were investigated. The probiotic attributes of B. subtilis was tested by conducting antagonism study, safety in Catla, in vitro immunomodulation and dietary immunomodulation. Results of these studies indicated the probiotic potential of the strain. From the preliminary dietary immunomodulation study, a dose of 10(9) B. subtilis cells g(-1) was selected for inclusion into diets for subsequent experiments. Experimental diets were prepared by adding B. subtilis (10(9) cells g(-1)), chitin (2%) and their combination to the basal diet. Different systemic and mucosal immunological parameters viz. oxygen radical production, myeloperoxidase content, lysozyme activity, total protein content and alkaline phosphatase activity showed significant enhancement (p<0.05) after 2 weeks of feeding with the combined diet. B. subtilis and chitin alone also significantly elevated most of the immune responses. All the diets significantly increased the resistance of Catla against E. tarda challenge. The highest post-challenge survival was observed in combined group (i.e. 63.33%). In conclusion, B. subtilis and chitin, alone or combined, had a health ameliorating effect in Catla. The results also collectively suggest the usefulness of applying a combined probiotic and immunostimulant supplemented diet to achieve greater benefits.

Rina Chakrabarti - One of the best experts on this subject based on the ideXlab platform.

  • Effect of temperature on digestive physiology, immune-modulatory parameters, and expression level of Hsp and LDH genes in Catla Catla (Hamilton, 1822)
    Aquaculture, 2017
    Co-Authors: Jaigopal Sharma, Samar Pal Singh, Rina Chakrabarti
    Abstract:

    Abstract Fish face the challenge of diurnal and seasonal fluctuations of temperature in culture and it is essential to understand their physiological responses to thermal stress. Indian major carp Catla Catla, Catla (7.52 ± 0.22 g) were acclimated at 28 ± 1 °C for 21 days and then exposed at six different temperatures: 10, 15, 20, 25, 28 and 35 °C. Blood and tissue samples were collected after 12 h and 7 days of reaching the assigned temperature. Significantly (p

  • achyranthes aspera stimulates the immunity and enhances the antigen clearance in Catla Catla
    International Immunopharmacology, 2006
    Co-Authors: Rina Chakrabarti, Rao.y. Vasudeva
    Abstract:

    Achyranthes aspera, an Indian medicinal plant (family: Amaranthaceae) was incorporated in artificial fish diet, and fed to Catla Catla Catla. After 4 weeks of feeding, fish were immunized with bovine serum albumin (BSA), spleen and blood were sampled on weekly intervals for four times after immunization. Antigen-specific antibody level in serum was determined by ELISA. Antigen clearance was determined in spleen by immuno-electron microscopy. Achyranthes has significantly (P<0.05) enhanced the BSA-specific antibody titers than the untreated control group throughout the study period. The efficiency of antigen clearance was also enhanced in C. Catla treated with Achyranthes.

  • Effects of UV-B radiation on the gills of Catla Catla during early development
    Toxicological & Environmental Chemistry, 2006
    Co-Authors: Jaigopal Sharma, Rina Chakrabarti
    Abstract:

    Scanning electron microscopic (SEM) study of gills of Catla Catla Catla (17-day-old) exposed to UV-B radiation (145 µW cm−2 at the water surface) for three different exposure times: 5, 10 and 15 min was conducted. Fish without UV-B exposure served as control. UV-B radiation damaged both gill filaments and lamellae. The intensity of damage was minimal in 5 min exposed fish, followed by 10 min exposed fish and maximal in 15 min. The gill epithelium was severely damaged in 15 min irradiated fish compared to control. Pavement cells (PVCs) were damaged and the numbers of microridges within PVCs decreased. The deep boundary of PVC was not clear. In some area of gill epithelium, PVCs were destroyed and mitochondrion-rich cells (MRCs) were exposed. The 5 min exposure reduced the number of microridges in the PVCs, but the boundary of PVCs was still visible. MRCs in the gill epithelium were not exposed in 5 min exposed fish. The damage to PVCs and subsequent exposure of MRCs in UV-B irradiated fish may hamper respi...

  • Achyranthes aspera stimulates the immunity and enhances the antigen clearance in Catla Catla.
    International immunopharmacology, 2005
    Co-Authors: Rina Chakrabarti, Rao.y. Vasudeva
    Abstract:

    Achyranthes aspera, an Indian medicinal plant (family: Amaranthaceae) was incorporated in artificial fish diet, and fed to Catla Catla Catla. After 4 weeks of feeding, fish were immunized with bovine serum albumin (BSA), spleen and blood were sampled on weekly intervals for four times after immunization. Antigen-specific antibody level in serum was determined by ELISA. Antigen clearance was determined in spleen by immuno-electron microscopy. Achyranthes has significantly (P

  • Stimulation of immunity in Indian major carp Catla Catla with herbal feed ingredients
    Fish & shellfish immunology, 2005
    Co-Authors: Y. Vasudeva Rao, Rina Chakrabarti
    Abstract:

    Abstract Catla Catla , Catla (150 ± 20 g) were fed a diet containing seed of Achyranthes aspera (0.5%) and control diet without A. aspera for four weeks prior to and after ip injection with chicken erythrocytes. Fish were sampled for four consecutive weeks after immunization. Hemagglutination antibody titers were significantly higher in the test group of fishes compared with the control group. Serum globulin levels were significantly ( P t -test P t -test P t -test A. aspera enhances the immunity of Catla.

Timothy Sangma - One of the best experts on this subject based on the ideXlab platform.

  • In Vitro and Dietary Effects of Chitin on Cellular and Humoral Immune Parameters of Catla, Catla Catla (Hamilton)
    Journal of the World Aquaculture Society, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Immunostimulants increase the immunocompetency and disease resistance of fish by enhancing immune responses. This study determined the in vitro and dietary effects of chitin on immune responses of Catla, Catla Catla. Catla head-kidney leukocytes were cultured in complete RPMI-1640 medium containing 0.01, 0.1, and 1.0 mg/mL of chitin. Leucocyte viability and different cellular immune responses, namely, respiratory burst, nitric oxide production, and leucocyte peroxidase content were analyzed in vitro. In the dietary experiment, chitin-supplemented diets (0.5, 1.0, and 2.0% of feed) were fed to Catla for 2 wk, and respiratory burst, serum lysozyme, and total protein contents were measured after 1 and 2 wk of feeding. Leucocytes incubated in vitro with chitin for 72 h maintained their viability. Chitin significantly (P 

  • in vitro and dietary effects of chitin on cellular and humoral immune parameters of Catla Catla Catla hamilton
    Journal of The World Aquaculture Society, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Immunostimulants increase the immunocompetency and disease resistance of fish by enhancing immune responses. This study determined the in vitro and dietary effects of chitin on immune responses of Catla, Catla Catla. Catla head-kidney leukocytes were cultured in complete RPMI-1640 medium containing 0.01, 0.1, and 1.0 mg/mL of chitin. Leucocyte viability and different cellular immune responses, namely, respiratory burst, nitric oxide production, and leucocyte peroxidase content were analyzed in vitro. In the dietary experiment, chitin-supplemented diets (0.5, 1.0, and 2.0% of feed) were fed to Catla for 2 wk, and respiratory burst, serum lysozyme, and total protein contents were measured after 1 and 2 wk of feeding. Leucocytes incubated in vitro with chitin for 72 h maintained their viability. Chitin significantly (P < 0.05) enhanced all the immune responses in vitro. The lowest dose showed the best response. Dietary chitin also significantly stimulated all the immune parameters in a dose-dependent manner with the highest dose showing the maximum responses. All the responses were higher after 1 wk of feeding and declined thereafter. The results suggest that chitin stimulates Catla cellular and humoral immune responses in vitro and in vivo.

  • dietary bacillus subtilis fptb13 and chitin single or combined modulate systemic and cutaneous mucosal immunity and resistance of Catla Catla Catla hamilton against edwardsiellosis
    Comparative Immunology Microbiology and Infectious Diseases, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Effects of dietary administration of Bacillus subtilis FPTB13 and chitin, single or combined, on the systemic immunity, mucosal immunity and resistance of Catla (Catla Catla) against Edwardsiella tarda infection were investigated. The probiotic attributes of B. subtilis was tested by conducting antagonism study, safety in Catla, in vitro immunomodulation and dietary immunomodulation. Results of these studies indicated the probiotic potential of the strain. From the preliminary dietary immunomodulation study, a dose of 10(9) B. subtilis cells g(-1) was selected for inclusion into diets for subsequent experiments. Experimental diets were prepared by adding B. subtilis (10(9) cells g(-1)), chitin (2%) and their combination to the basal diet. Different systemic and mucosal immunological parameters viz. oxygen radical production, myeloperoxidase content, lysozyme activity, total protein content and alkaline phosphatase activity showed significant enhancement (p<0.05) after 2 weeks of feeding with the combined diet. B. subtilis and chitin alone also significantly elevated most of the immune responses. All the diets significantly increased the resistance of Catla against E. tarda challenge. The highest post-challenge survival was observed in combined group (i.e. 63.33%). In conclusion, B. subtilis and chitin, alone or combined, had a health ameliorating effect in Catla. The results also collectively suggest the usefulness of applying a combined probiotic and immunostimulant supplemented diet to achieve greater benefits.

  • Dietary Bacillus subtilis FPTB13 and chitin, single or combined, modulate systemic and cutaneous mucosal immunity and resistance of Catla, Catla Catla (Hamilton) against edwardsiellosis.
    Comparative immunology microbiology and infectious diseases, 2015
    Co-Authors: Timothy Sangma, Dibyendu Kamilya
    Abstract:

    Effects of dietary administration of Bacillus subtilis FPTB13 and chitin, single or combined, on the systemic immunity, mucosal immunity and resistance of Catla (Catla Catla) against Edwardsiella tarda infection were investigated. The probiotic attributes of B. subtilis was tested by conducting antagonism study, safety in Catla, in vitro immunomodulation and dietary immunomodulation. Results of these studies indicated the probiotic potential of the strain. From the preliminary dietary immunomodulation study, a dose of 10(9) B. subtilis cells g(-1) was selected for inclusion into diets for subsequent experiments. Experimental diets were prepared by adding B. subtilis (10(9) cells g(-1)), chitin (2%) and their combination to the basal diet. Different systemic and mucosal immunological parameters viz. oxygen radical production, myeloperoxidase content, lysozyme activity, total protein content and alkaline phosphatase activity showed significant enhancement (p

  • Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla Catla (Hamilton) In Vitro
    Probiotics and Antimicrobial Proteins, 2015
    Co-Authors: Dibyendu Kamilya, Timothy Sangma, Supratim Chowdhury, Arunjyoti Baruah, Prasenjit Pal
    Abstract:

    In the present study, we investigated the in vitro immunostimulatory effects of inactivated form of two potential probiotics, Bacillus amyloliquefaciens FPTB16 and B. subtilis FPTB13 in Catla ( Catla Catla ). Catla head kidney leukocytes (HK) were incubated with the bacteria alive or inactivated with heat shock (2 h at 60 °C), UV light (for 2.5 h) and formalin (1.0 %, v/v) treatment (for 24 h at 4 °C) at different concentrations (10^7, 10^8 and 10^9 cells ml^−1). After incubation, different cellular immune parameters such as respiratory burst activity, nitric oxide production, leukocyte peroxidase content and proliferative response were analyzed. The inactivated probiotic preparations stimulated all the cellular immune parameters of Catla HK leukocytes in vitro. Among the different inactivated preparations, heat-treated form exhibited the best result. The lowest dose (10^7 cells ml^−1) of both the strains showed the maximum stimulation. The results collectively suggest the efficacy of inactivated preparations to be used as immunostimulant in aquaculture.

Jaigopal Sharma - One of the best experts on this subject based on the ideXlab platform.

  • Effect of temperature on digestive physiology, immune-modulatory parameters, and expression level of Hsp and LDH genes in Catla Catla (Hamilton, 1822)
    Aquaculture, 2017
    Co-Authors: Jaigopal Sharma, Samar Pal Singh, Rina Chakrabarti
    Abstract:

    Abstract Fish face the challenge of diurnal and seasonal fluctuations of temperature in culture and it is essential to understand their physiological responses to thermal stress. Indian major carp Catla Catla, Catla (7.52 ± 0.22 g) were acclimated at 28 ± 1 °C for 21 days and then exposed at six different temperatures: 10, 15, 20, 25, 28 and 35 °C. Blood and tissue samples were collected after 12 h and 7 days of reaching the assigned temperature. Significantly (p

  • Effects of UV-B radiation on the gills of Catla Catla during early development
    Toxicological & Environmental Chemistry, 2006
    Co-Authors: Jaigopal Sharma, Rina Chakrabarti
    Abstract:

    Scanning electron microscopic (SEM) study of gills of Catla Catla Catla (17-day-old) exposed to UV-B radiation (145 µW cm−2 at the water surface) for three different exposure times: 5, 10 and 15 min was conducted. Fish without UV-B exposure served as control. UV-B radiation damaged both gill filaments and lamellae. The intensity of damage was minimal in 5 min exposed fish, followed by 10 min exposed fish and maximal in 15 min. The gill epithelium was severely damaged in 15 min irradiated fish compared to control. Pavement cells (PVCs) were damaged and the numbers of microridges within PVCs decreased. The deep boundary of PVC was not clear. In some area of gill epithelium, PVCs were destroyed and mitochondrion-rich cells (MRCs) were exposed. The 5 min exposure reduced the number of microridges in the PVCs, but the boundary of PVCs was still visible. MRCs in the gill epithelium were not exposed in 5 min exposed fish. The damage to PVCs and subsequent exposure of MRCs in UV-B irradiated fish may hamper respi...

  • role of stocking density on growth and survival of Catla Catla Catla and rohu labeo rohita larvae and water quality in a recirculating system
    Journal of Applied Aquaculture, 2003
    Co-Authors: Jaigopal Sharma, Rina Chakrabarti
    Abstract:

    ABSTRACT Catla, Catla Catla, and rohu, Labeo rohita, fry were cultured at 6,667,8,333, and 10,000/m3 in 15-L aquaria in recirculating systems for 30 days. Larvae were fed with exogenous live plankton. Cultures at 6,667 and 8,333 larvae/m3 showed significantly (P <0.05) higher survival and growth than larvae stocked at 10,000 larvae/m3 for both species. Food was more efficiently used in low stocking density, as evident from the significantly (P <0.05) lower values of feed conversion ratio in lower density compared to those for high stocking density. Specific growth rate of both species was high in the early stage and gradually declined along with the ontogenic development. Dissolved oxygen level was higher in the low density system than in the high density one. Values of phosphate and COD increased during the experiment. Ammonia, nitrite, phosphate, and COD levels were significantly (P <0.05) higher in the 10,000 larvae/m3 density system than in the other two systems for both species. Considering the survi...

  • Role of Stocking Density on Growth and Survival of Catla, Catla Catla, and Rohu, Labeo rohita, Larvae and Water Quality in a Recirculating System
    Journal of Applied Aquaculture, 2003
    Co-Authors: Jaigopal Sharma, Rina Chakrabarti
    Abstract:

    ABSTRACT Catla, Catla Catla, and rohu, Labeo rohita, fry were cultured at 6,667,8,333, and 10,000/m3 in 15-L aquaria in recirculating systems for 30 days. Larvae were fed with exogenous live plankton. Cultures at 6,667 and 8,333 larvae/m3 showed significantly (P

A.s. Sahul Hameed - One of the best experts on this subject based on the ideXlab platform.

  • Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and Catla, Catla Catla (Hamilton)
    Journal of fish diseases, 2009
    Co-Authors: V.p. Ishaq Ahmed, Vaibhav Chandra, R. Sudhakaran, S. Rajesh Kumar, M. Sarathi, V. Sarath Babu, B Ramesh, A.s. Sahul Hameed
    Abstract:

    Two new cell lines, designated RE and CB, were derived from the eye of rohu, Labeo rohita, and the brain of Catla, Catla Catla, respectively. The cell lines were maintained in Leibovitz's L-15 supplemented with 20% foetal bovine serum. The RE cell line was sub-cultured for more than 70 passages and the CB cell line for more than 35 passages. The RE cells are rounded and consist predominantly of epithelial cells. The CB cell line consists of predominantly fibroblastic-like cells. Both cell lines are able to grow at temperatures between 25 and 32 degrees C with an optimum of 28 degrees C. The growth rate of the cells increased as the foetal bovine serum concentration increased from 2% to 20% at 28 degrees C, with optimum growth at concentrations of 15% or 20% foetal bovine serum. The cells were successfully cryopreserved and revived at different passage levels. The cell lines were not susceptible to four marine fish viruses. Extracellular products from Aeromonas sp. were toxic to the cell lines. When the cells were transfected with plasmid eukaryotic green fluorescent protein (pEGFP [Clontech, Carlsbad, CA, USA]) vector DNA, a significant fluorescent signal was observed suggesting that these cell lines could be a useful tool for transgenic and genetic manipulation studies. Polymerase chain reaction amplification of mitochondrial 12S rRNA from rohu and Catla confirmed that the cell lines originated from these fish species. The cell lines were further characterized by immunocytochemistry using confocal laser scanning microscopy.