The Experts below are selected from a list of 57285 Experts worldwide ranked by ideXlab platform
Lauren D Zarzar - One of the best experts on this subject based on the ideXlab platform.
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photothermally triggered actuation of hybrid materials as a new platform for in vitro Cell Manipulation
Nature Communications, 2017Co-Authors: Amy Sutton, Tanya Shirman, Jaakko V I Timonen, Grant T England, Mathias Kolle, Thomas C Ferrante, Lauren D Zarzar, Elizabeth StrongAbstract:Mechanical forces in the Cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual Cells and Cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on Cells, particularly with sub-Cellular resolution, in controlled in vitro experiments. Here we report a new type of active Cell culture material that allows highly localized, directional and reversible deformation of the Cell growth substrate, with control at scales ranging from the entire surface to the subCellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single Cell micro-Manipulation and 2D Cell sheet mechanical stimulation techniques. Mechanical forces within the Cell’s environment play a crucial role in their growth, differentiation and behaviour. Here, the authors develop a photothermal responsive Cell culture substrate for the assessment of how Cell growth can be affected by manipulating the strain profile of the substrate.
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photothermally triggered actuation of hybrid materials as a new platform for in vitro Cell Manipulation
Nature Communications, 2017Co-Authors: Amy Sutton, Tanya Shirman, Jaakko V I Timonen, Grant T England, Mathias Kolle, Thomas C Ferrante, Lauren D Zarzar, Philseok KimAbstract:Mechanical forces in the Cell's natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual Cells and Cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on Cells, particularly with sub-Cellular resolution, in controlled in vitro experiments. Here we report a new type of active Cell culture material that allows highly localized, directional and reversible deformation of the Cell growth substrate, with control at scales ranging from the entire surface to the subCellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single Cell micro-Manipulation and 2D Cell sheet mechanical stimulation techniques.
Jaakko V I Timonen - One of the best experts on this subject based on the ideXlab platform.
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photothermally triggered actuation of hybrid materials as a new platform for in vitro Cell Manipulation
Nature Communications, 2017Co-Authors: Amy Sutton, Tanya Shirman, Jaakko V I Timonen, Grant T England, Mathias Kolle, Thomas C Ferrante, Lauren D Zarzar, Elizabeth StrongAbstract:Mechanical forces in the Cell’s natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual Cells and Cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on Cells, particularly with sub-Cellular resolution, in controlled in vitro experiments. Here we report a new type of active Cell culture material that allows highly localized, directional and reversible deformation of the Cell growth substrate, with control at scales ranging from the entire surface to the subCellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single Cell micro-Manipulation and 2D Cell sheet mechanical stimulation techniques. Mechanical forces within the Cell’s environment play a crucial role in their growth, differentiation and behaviour. Here, the authors develop a photothermal responsive Cell culture substrate for the assessment of how Cell growth can be affected by manipulating the strain profile of the substrate.
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photothermally triggered actuation of hybrid materials as a new platform for in vitro Cell Manipulation
Nature Communications, 2017Co-Authors: Amy Sutton, Tanya Shirman, Jaakko V I Timonen, Grant T England, Mathias Kolle, Thomas C Ferrante, Lauren D Zarzar, Philseok KimAbstract:Mechanical forces in the Cell's natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual Cells and Cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on Cells, particularly with sub-Cellular resolution, in controlled in vitro experiments. Here we report a new type of active Cell culture material that allows highly localized, directional and reversible deformation of the Cell growth substrate, with control at scales ranging from the entire surface to the subCellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single Cell micro-Manipulation and 2D Cell sheet mechanical stimulation techniques.
Win Tun Latt - One of the best experts on this subject based on the ideXlab platform.
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Autofocusing and Polar Body Detection in Automated Cell Manipulation
IEEE Transactions on Biomedical Engineering, 2017Co-Authors: Zenan Wang, Chen Feng, Win Tun LattAbstract:Autofocusing and feature detection are two essential processes for performing automated biological Cell Manipulation tasks. In this paper, we have introduced a technique capable of focusing on a holding pipette and a mammalian Cell under a bright-field microscope automatically, and a technique that can detect and track the presence and orientation of the polar body of an oocyte that is rotated at the tip of a micropipette. Both algorithms were evaluated by using mouse oocytes. Experimental results show that both algorithms achieve very high success rates: 100% and 96%. As robust and accurate image processing methods, they can be widely applied to perform various automated biological Cell Manipulations.
Aaron T Ohta - One of the best experts on this subject based on the ideXlab platform.
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an opto thermocapillary Cell micromanipulator
Lab on a Chip, 2013Co-Authors: Wenqi Hu, Aaron T OhtaAbstract:An opto-thermocapillary micromanipulator (OTMm) capable of single-Cell Manipulation and patterning is presented here. The OTMm uses a near-infrared laser focused on an ITO substrate to induce thermocapillary convection that can trap and transport living Cells with forces of up to 40 pN. The OTMm complements other Cell-Manipulation technologies, such as optical tweezers and dielectrophoresis, as it is less dependent upon the optical and electrical properties of the working environment, and can function in many types of Cell culture media. The OTMm was used to construct single-Cell matrices in two popular hydrogels: PEGDA and agarose. High viability rates were observed in both hydrogels, and Cells patterned in agarose spread and migrated during subsequent culturing.
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phototransistor based optoelectronic tweezers for dynamic Cell Manipulation in Cell culture media
Lab on a Chip, 2010Co-Authors: Hsanyin Hsu, Aaron T Ohta, Peiyu Chiou, Arash Jamshidi, Steven L NealeAbstract:Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel Manipulation of micro-particles and Cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370–372).1 However, the conventional OET device cannot operate in Cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500× higher photoconductivity than amorphous silicon. Efficient Cell trapping of live HeLa and Jurkat Cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a Cell transport speed of 33 µm/sec, indicating a force of 14.5 pN. Optical concentration of Cells and real-time control of individually addressable Cell arrays have also been realized. Precise control of separation between two Cells has also been demonstrated. We envision a new platform for single Cell studies using Ph-OET.
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Optoelectronic Tweezers as a Tool for Parallel Single-Cell Manipulation and Stimulation
IEEE Transactions on Biomedical Circuits and Systems, 2009Co-Authors: Justin K. Valley, Aaron T Ohta, Arash Jamshidi, Steven L Neale, Ming C. WuAbstract:Optoelectronic tweezers (OET) is a promising approach for the parallel Manipulation of single Cells for a variety of biological applications. By combining the Manipulation capabilities of OET with other relevant biological techniques (such as Cell lysis and electroporation), one can realize a true parallel, single-Cell diagnostic and stimulation tool. Here, we demonstrate the utility of the OET device by integrating it onto single-chip systems capable of performing in-situ, electrode-based electroporation/lysis, individual Cell, light-induced lysis, and light-induced electroporation.
R M Westervelt - One of the best experts on this subject based on the ideXlab platform.
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integrated Cell Manipulation system cmos microfluidic hybrid
Lab on a Chip, 2007Co-Authors: Hakho Lee, Yong Liu, Donhee Ham, R M WesterveltAbstract:Manipulation of biological Cells using a CMOS/microfluidic hybrid system is demonstrated. The hybrid system starts with a custom-designed CMOS (complementary metal-oxide semiconductor) chip fabricated in a semiconductor foundry. A microfluidic channel is post-fabricated on top of the CMOS chip to provide biocompatible environments. The motion of individual biological Cells that are tagged with magnetic beads is directly controlled by the CMOS chip that generates microscopic magnetic field patterns using an on-chip array of micro-electromagnets. Furthermore, the CMOS chip allows high-speed and programmable reconfiguration of the magnetic fields, substantially increasing the Manipulation capability of the hybrid system. Extending from previous work that verified the concept of the hybrid system, this paper reports a set of Manipulation experiments with biological Cells, which further confirms the advantage of the hybrid approach. To enhance the biocompatibility of the system, the microfluidic channel is redesigned and the temperature of the device is monitored by on-chip sensors. Combining microelectronics and microfluidics, the CMOS/microfluidic hybrid system presents a new model for a Cell Manipulation platform in biological and biomedical applications.
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dielectrophoresis tweezers for single Cell Manipulation
Biomedical Microdevices, 2006Co-Authors: Thomas Hunt, R M WesterveltAbstract:Positioning single Cells is of utmost importance in areas of biomedical research as diverse as in vitro fertilization, Cell-Cell interaction, Cell adhesion, embryology, microbiology, stem Cell research, and single Cell transfection. Here we describe dielectrophoretic tweezers, a sharp glass tip with electrodes on either side, capable of trapping single Cells with electric fields. Mounted on a micromanipulator, dielectrophoresis tweezers can position a single Cell in three dimensions, holding the Cell against fluid flow of hundreds of microns per second with more than 10 pN of force. We model the electric field produced by the tweezers and the field produced by coaxial microelectrodes. We show that Cells are trapped without harm while they divide in the trap. In addition, dielectrophoretic tweezers offer the possibility for trapping, electroporating, and microinjecting a single Cell with one probe.